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As described by Lowe et al. (2000), midgestation embryos carrying both the Hoxb6-Cre transgene and the R26R lacZ reporter gene (Soriano 1999) exhibit X-Gal staining in lateral plate mesoderm but not somite-derived mesoderm of the trunk. In whole-mount skin preparations from P1.5 or P4.5 neonatal animals, we observed a ventral band of dark X-Gal staining corresponding to lateral plate-derived dermis, which represents 63% of the total circumference (Figure 8A). However, in parallel preparations from at/at mice, the ventral pheomelanin domain represents 47% of the total skin circumference; therefore, the proportions of total skin circumference occupied by dorsal eumelanin and somite-derived dermis are 53% and 37%, respectively (Figure 8B). These results indicate that the pigmentation boundary is clearly distinct from, and more ventral to, the boundary between lateral plate- and somite-derived dermis."}

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{"project":"2_test","denotations":[{"id":"14737183-10686603-84903012","span":{"begin":235,"end":239},"obj":"10686603"},{"id":"14737183-10686603-84903013","span":{"begin":271,"end":275},"obj":"10686603"},{"id":"14737183-9916792-84903014","span":{"begin":378,"end":382},"obj":"9916792"},{"id":"T74039","span":{"begin":235,"end":239},"obj":"10686603"},{"id":"T14949","span":{"begin":271,"end":275},"obj":"10686603"},{"id":"T41736","span":{"begin":378,"end":382},"obj":"9916792"}],"text":"To examine directly the relationship between the pigmentation boundary and dermis derived from lateral plate mesoderm, we made use of a Cre transgene driven by the Hoxb6 promoter that was developed by Kuehn and colleagues (Lowe et al. 2000). As described by Lowe et al. (2000), midgestation embryos carrying both the Hoxb6-Cre transgene and the R26R lacZ reporter gene (Soriano 1999) exhibit X-Gal staining in lateral plate mesoderm but not somite-derived mesoderm of the trunk. In whole-mount skin preparations from P1.5 or P4.5 neonatal animals, we observed a ventral band of dark X-Gal staining corresponding to lateral plate-derived dermis, which represents 63% of the total circumference (Figure 8A). However, in parallel preparations from at/at mice, the ventral pheomelanin domain represents 47% of the total skin circumference; therefore, the proportions of total skin circumference occupied by dorsal eumelanin and somite-derived dermis are 53% and 37%, respectively (Figure 8B). These results indicate that the pigmentation boundary is clearly distinct from, and more ventral to, the boundary between lateral plate- and somite-derived dermis."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T6825","span":{"begin":49,"end":61},"obj":"GO_PATO_EXT:biological_pigmentation_process_or_quality"},{"id":"T6826","span":{"begin":75,"end":81},"obj":"UBERON:0002067"},{"id":"T6827","span":{"begin":95,"end":117},"obj":"UBERON:0003081"},{"id":"T6828","span":{"begin":140,"end":149},"obj":"SO_EXT:0000902"},{"id":"T6829","span":{"begin":164,"end":169},"obj":"PR_EXT:000008703"},{"id":"T6830","span":{"begin":170,"end":178},"obj":"SO_EXT:0000167"},{"id":"T6831","span":{"begin":281,"end":290},"obj":"GO:0007565"},{"id":"T6832","span":{"begin":291,"end":298},"obj":"UBERON:0000922"},{"id":"T6833","span":{"begin":317,"end":322},"obj":"PR_EXT:000008703"},{"id":"T6834","span":{"begin":327,"end":336},"obj":"SO_EXT:0000902"},{"id":"T6835","span":{"begin":350,"end":354},"obj":"PR_EXT:000033987"},{"id":"T6836","span":{"begin":364,"end":368},"obj":"SO_EXT:0000704"},{"id":"T6837","span":{"begin":392,"end":397},"obj":"CHEBI:75055"},{"id":"T6838","span":{"begin":410,"end":432},"obj":"UBERON:0003081"},{"id":"T6839","span":{"begin":441,"end":464},"obj":"UBERON:0003077"},{"id":"T6840","span":{"begin":472,"end":477},"obj":"UBERON:0002100"},{"id":"T6841","span":{"begin":494,"end":498},"obj":"UBERON_EXT:zone_of_skin_or_skin_of_body"},{"id":"T6842","span":{"begin":530,"end":538},"obj":"PATO_UBERON_EXT:neonate_or_newborn"},{"id":"T6843","span":{"begin":539,"end":546},"obj":"NCBITaxon:33208"},{"id":"T6844","span":{"begin":583,"end":588},"obj":"CHEBI:75055"},{"id":"T6845","span":{"begin":615,"end":628},"obj":"UBERON:0003081"},{"id":"T6846","span":{"begin":637,"end":643},"obj":"UBERON:0002067"},{"id":"T6847","span":{"begin":751,"end":755},"obj":"NCBITaxon:10088"},{"id":"T6848","span":{"begin":769,"end":780},"obj":"CHEBI:81393"},{"id":"T6849","span":{"begin":816,"end":820},"obj":"UBERON_EXT:zone_of_skin_or_skin_of_body"},{"id":"T6850","span":{"begin":872,"end":876},"obj":"UBERON_EXT:zone_of_skin_or_skin_of_body"},{"id":"T6851","span":{"begin":910,"end":919},"obj":"CHEBI:24009"},{"id":"T6852","span":{"begin":924,"end":930},"obj":"UBERON:0002329"},{"id":"T6853","span":{"begin":939,"end":945},"obj":"UBERON:0002067"},{"id":"T6854","span":{"begin":1021,"end":1033},"obj":"GO_PATO_EXT:biological_pigmentation_process_or_quality"},{"id":"T6855","span":{"begin":1111,"end":1124},"obj":"UBERON:0003081"},{"id":"T6856","span":{"begin":1130,"end":1136},"obj":"UBERON:0002329"},{"id":"T6857","span":{"begin":1145,"end":1151},"obj":"UBERON:0002067"}],"text":"To examine directly the relationship between the pigmentation boundary and dermis derived from lateral plate mesoderm, we made use of a Cre transgene driven by the Hoxb6 promoter that was developed by Kuehn and colleagues (Lowe et al. 2000). As described by Lowe et al. (2000), midgestation embryos carrying both the Hoxb6-Cre transgene and the R26R lacZ reporter gene (Soriano 1999) exhibit X-Gal staining in lateral plate mesoderm but not somite-derived mesoderm of the trunk. In whole-mount skin preparations from P1.5 or P4.5 neonatal animals, we observed a ventral band of dark X-Gal staining corresponding to lateral plate-derived dermis, which represents 63% of the total circumference (Figure 8A). However, in parallel preparations from at/at mice, the ventral pheomelanin domain represents 47% of the total skin circumference; therefore, the proportions of total skin circumference occupied by dorsal eumelanin and somite-derived dermis are 53% and 37%, respectively (Figure 8B). These results indicate that the pigmentation boundary is clearly distinct from, and more ventral to, the boundary between lateral plate- and somite-derived dermis."}