Id |
Subject |
Object |
Predicate |
Lexical cue |
T5465 |
0-10 |
NN |
denotes |
Expression |
T5466 |
11-13 |
IN |
denotes |
of |
T5467 |
14-19 |
NN |
denotes |
Tbx15 |
T5468 |
20-23 |
CC |
denotes |
and |
T5469 |
24-30 |
NN |
denotes |
Agouti |
T5470 |
30-273 |
sentence |
denotes |
Previous studies by Agulnik et al. (1998) using whole-mount in situ hybridization described expression of Tbx15 as first detectable at E9.5 in the limb buds, progressing to the branchial arches, flanks, and craniofacial regions through E12.5. |
T5471 |
31-39 |
JJ |
denotes |
Previous |
T5472 |
40-47 |
NNS |
denotes |
studies |
T5473 |
113-122 |
VBD |
denotes |
described |
T5474 |
48-50 |
IN |
denotes |
by |
T5475 |
51-58 |
NNP |
denotes |
Agulnik |
T5476 |
59-61 |
FW |
denotes |
et |
T5477 |
62-65 |
FW |
denotes |
al. |
T5478 |
66-67 |
-LRB- |
denotes |
( |
T5479 |
67-71 |
CD |
denotes |
1998 |
T5480 |
71-72 |
-RRB- |
denotes |
) |
T5481 |
73-78 |
VBG |
denotes |
using |
T5482 |
79-84 |
JJ |
denotes |
whole |
T5483 |
85-90 |
NN |
denotes |
mount |
T5484 |
84-85 |
HYPH |
denotes |
- |
T5485 |
99-112 |
NN |
denotes |
hybridization |
T5486 |
91-93 |
FW |
denotes |
in |
T5487 |
94-98 |
FW |
denotes |
situ |
T5488 |
123-133 |
NN |
denotes |
expression |
T5489 |
134-136 |
IN |
denotes |
of |
T5490 |
137-142 |
NN |
denotes |
Tbx15 |
T5491 |
143-145 |
IN |
denotes |
as |
T5492 |
146-151 |
RB |
denotes |
first |
T5493 |
152-162 |
JJ |
denotes |
detectable |
T5494 |
163-165 |
IN |
denotes |
at |
T5495 |
166-170 |
NN |
denotes |
E9.5 |
T5496 |
171-173 |
IN |
denotes |
in |
T5497 |
174-177 |
DT |
denotes |
the |
T5498 |
183-187 |
NNS |
denotes |
buds |
T5499 |
178-182 |
NN |
denotes |
limb |
T5500 |
187-189 |
, |
denotes |
, |
T5501 |
189-200 |
VBG |
denotes |
progressing |
T5502 |
201-203 |
IN |
denotes |
to |
T5503 |
204-207 |
DT |
denotes |
the |
T5504 |
218-224 |
NNS |
denotes |
arches |
T5505 |
208-217 |
JJ |
denotes |
branchial |
T5506 |
224-226 |
, |
denotes |
, |
T5507 |
226-232 |
NNS |
denotes |
flanks |
T5508 |
232-234 |
, |
denotes |
, |
T5509 |
234-237 |
CC |
denotes |
and |
T5510 |
238-250 |
JJ |
denotes |
craniofacial |
T5511 |
251-258 |
NNS |
denotes |
regions |
T5512 |
259-266 |
IN |
denotes |
through |
T5513 |
267-272 |
NN |
denotes |
E12.5 |
T5514 |
272-273 |
. |
denotes |
. |
T5515 |
273-650 |
sentence |
denotes |
To investigate this pattern in more detail, we hybridized a Tbx15 mRNA probe to a series of transverse sections at E12.5 and observed expression in multiple mesenchymal tissues of the head, trunk, and developing limbs (Figure 4A), much of which is consistent with the skull, cervical vertebrae, and limb malformations reported for mice carrying the original droopy ear allele. |
T5516 |
274-276 |
TO |
denotes |
To |
T5517 |
277-288 |
VB |
denotes |
investigate |
T5518 |
321-331 |
VBD |
denotes |
hybridized |
T5519 |
289-293 |
DT |
denotes |
this |
T5520 |
294-301 |
NN |
denotes |
pattern |
T5521 |
302-304 |
IN |
denotes |
in |
T5522 |
305-309 |
JJR |
denotes |
more |
T5523 |
310-316 |
NN |
denotes |
detail |
T5524 |
316-318 |
, |
denotes |
, |
T5525 |
318-320 |
PRP |
denotes |
we |
T5526 |
332-333 |
DT |
denotes |
a |
T5527 |
345-350 |
NN |
denotes |
probe |
T5528 |
334-339 |
NN |
denotes |
Tbx15 |
T5529 |
340-344 |
NN |
denotes |
mRNA |
T5530 |
351-353 |
IN |
denotes |
to |
T5531 |
354-355 |
DT |
denotes |
a |
T5532 |
356-362 |
NN |
denotes |
series |
T5533 |
363-365 |
IN |
denotes |
of |
T5534 |
366-376 |
JJ |
denotes |
transverse |
T5535 |
377-385 |
NNS |
denotes |
sections |
T5536 |
386-388 |
IN |
denotes |
at |
T5537 |
389-394 |
NN |
denotes |
E12.5 |
T5538 |
395-398 |
CC |
denotes |
and |
T5539 |
399-407 |
VBD |
denotes |
observed |
T5540 |
408-418 |
NN |
denotes |
expression |
T5541 |
419-421 |
IN |
denotes |
in |
T5542 |
422-430 |
JJ |
denotes |
multiple |
T5543 |
443-450 |
NNS |
denotes |
tissues |
T5544 |
431-442 |
JJ |
denotes |
mesenchymal |
T5545 |
451-453 |
IN |
denotes |
of |
T5546 |
454-457 |
DT |
denotes |
the |
T5547 |
458-462 |
NN |
denotes |
head |
T5548 |
462-464 |
, |
denotes |
, |
T5549 |
464-469 |
NN |
denotes |
trunk |
T5550 |
469-471 |
, |
denotes |
, |
T5551 |
471-474 |
CC |
denotes |
and |
T5552 |
475-485 |
VBG |
denotes |
developing |
T5553 |
486-491 |
NNS |
denotes |
limbs |
T5554 |
492-493 |
-LRB- |
denotes |
( |
T5555 |
493-499 |
NN |
denotes |
Figure |
T5556 |
500-502 |
CD |
denotes |
4A |
T5557 |
502-503 |
-RRB- |
denotes |
) |
T5558 |
503-505 |
, |
denotes |
, |
T5559 |
505-509 |
JJ |
denotes |
much |
T5560 |
519-521 |
VBZ |
denotes |
is |
T5561 |
510-512 |
IN |
denotes |
of |
T5562 |
513-518 |
WDT |
denotes |
which |
T5563 |
522-532 |
JJ |
denotes |
consistent |
T5564 |
533-537 |
IN |
denotes |
with |
T5565 |
538-541 |
DT |
denotes |
the |
T5566 |
578-591 |
NNS |
denotes |
malformations |
T5567 |
542-547 |
NN |
denotes |
skull |
T5568 |
547-549 |
, |
denotes |
, |
T5569 |
549-557 |
JJ |
denotes |
cervical |
T5570 |
558-567 |
NNS |
denotes |
vertebrae |
T5571 |
567-569 |
, |
denotes |
, |
T5572 |
569-572 |
CC |
denotes |
and |
T5573 |
573-577 |
NN |
denotes |
limb |
T5574 |
592-600 |
VBN |
denotes |
reported |
T5575 |
601-604 |
IN |
denotes |
for |
T5576 |
605-609 |
NNS |
denotes |
mice |
T5577 |
610-618 |
VBG |
denotes |
carrying |
T5578 |
619-622 |
DT |
denotes |
the |
T5579 |
643-649 |
NN |
denotes |
allele |
T5580 |
623-631 |
JJ |
denotes |
original |
T5581 |
632-638 |
JJ |
denotes |
droopy |
T5582 |
639-642 |
NN |
denotes |
ear |
T5583 |
649-650 |
. |
denotes |
. |
T5584 |
650-1601 |
sentence |
denotes |
Figure 4 Developmental Expression of Tbx15
(A) At E12.5, transverse sections at different levels show expression in head mesenchyme (a and b); myotome, occipital, and periocular mesenchyme (b); palatal shelf, cervical sclerotome, and nasal cartilage (c); maxillary and mandibular processes (d); limbs (e); and myotome and lateral mesenchyme (e and f) (scale bars = 500 μm).
(B) Transverse sections through the flank at different times show expression in lateral mesenchyme (E11.5), expanding dorsally at E12.5, and both ventrally and dorsally at E13.5, detectable in loose mesenchyme underlying the dermis and the abdominal and subcutaneous muscles (scale bar = 500 μm). At P3.5, Tbx15 is expressed in the entire dermis and is most strongly expressed in dermal sheaths (scale bar = 200 μm). We were particularly interested in determining the exact nature of the embryonic flank expression relative to the ventralized phenotype of adult deH/deH mice. |
T5585 |
1443-1445 |
PRP |
denotes |
We |
T5586 |
1446-1450 |
VBD |
denotes |
were |
T5587 |
1451-1463 |
RB |
denotes |
particularly |
T5588 |
1464-1474 |
JJ |
denotes |
interested |
T5589 |
1475-1477 |
IN |
denotes |
in |
T5590 |
1478-1489 |
VBG |
denotes |
determining |
T5591 |
1490-1493 |
DT |
denotes |
the |
T5592 |
1500-1506 |
NN |
denotes |
nature |
T5593 |
1494-1499 |
JJ |
denotes |
exact |
T5594 |
1507-1509 |
IN |
denotes |
of |
T5595 |
1510-1513 |
DT |
denotes |
the |
T5596 |
1530-1540 |
NN |
denotes |
expression |
T5597 |
1514-1523 |
JJ |
denotes |
embryonic |
T5598 |
1524-1529 |
NN |
denotes |
flank |
T5599 |
1541-1549 |
JJ |
denotes |
relative |
T5600 |
1550-1552 |
IN |
denotes |
to |
T5601 |
1553-1556 |
DT |
denotes |
the |
T5602 |
1569-1578 |
NN |
denotes |
phenotype |
T5603 |
1557-1568 |
VBN |
denotes |
ventralized |
T5604 |
1579-1581 |
IN |
denotes |
of |
T5605 |
1582-1587 |
JJ |
denotes |
adult |
T5606 |
1596-1600 |
NNS |
denotes |
mice |
T5607 |
1588-1591 |
NN |
denotes |
deH |
T5608 |
1592-1595 |
NN |
denotes |
deH |
T5609 |
1591-1592 |
SYM |
denotes |
/ |
T5610 |
1600-1601 |
. |
denotes |
. |
T5611 |
1601-1833 |
sentence |
denotes |
Transverse abdominal sections from different times during development reveal a dorsolateral band of expression in the superficial mesenchyme at E11.5 that broadens both dorsally and ventrally over the next several days (Figure 4B). |
T5612 |
1602-1612 |
JJ |
denotes |
Transverse |
T5613 |
1623-1631 |
NNS |
denotes |
sections |
T5614 |
1613-1622 |
JJ |
denotes |
abdominal |
T5615 |
1672-1678 |
VBP |
denotes |
reveal |
T5616 |
1632-1636 |
IN |
denotes |
from |
T5617 |
1637-1646 |
JJ |
denotes |
different |
T5618 |
1647-1652 |
NNS |
denotes |
times |
T5619 |
1653-1659 |
IN |
denotes |
during |
T5620 |
1660-1671 |
NN |
denotes |
development |
T5621 |
1679-1680 |
DT |
denotes |
a |
T5622 |
1694-1698 |
NN |
denotes |
band |
T5623 |
1681-1693 |
JJ |
denotes |
dorsolateral |
T5624 |
1699-1701 |
IN |
denotes |
of |
T5625 |
1702-1712 |
NN |
denotes |
expression |
T5626 |
1713-1715 |
IN |
denotes |
in |
T5627 |
1716-1719 |
DT |
denotes |
the |
T5628 |
1732-1742 |
NN |
denotes |
mesenchyme |
T5629 |
1720-1731 |
JJ |
denotes |
superficial |
T5630 |
1743-1745 |
IN |
denotes |
at |
T5631 |
1746-1751 |
NN |
denotes |
E11.5 |
T5632 |
1752-1756 |
WDT |
denotes |
that |
T5633 |
1757-1765 |
VBZ |
denotes |
broadens |
T5634 |
1766-1770 |
CC |
denotes |
both |
T5635 |
1771-1779 |
RB |
denotes |
dorsally |
T5636 |
1780-1783 |
CC |
denotes |
and |
T5637 |
1784-1793 |
RB |
denotes |
ventrally |
T5638 |
1794-1798 |
IN |
denotes |
over |
T5639 |
1799-1802 |
DT |
denotes |
the |
T5640 |
1816-1820 |
NNS |
denotes |
days |
T5641 |
1803-1807 |
JJ |
denotes |
next |
T5642 |
1808-1815 |
JJ |
denotes |
several |
T5643 |
1821-1822 |
-LRB- |
denotes |
( |
T5644 |
1822-1828 |
NN |
denotes |
Figure |
T5645 |
1829-1831 |
CD |
denotes |
4B |
T5646 |
1831-1832 |
-RRB- |
denotes |
) |
T5647 |
1832-1833 |
. |
denotes |
. |
T5648 |
1833-2031 |
sentence |
denotes |
By E13.5, the developing dermis has become separated from the loose mesenchyme by a subcutaneous muscle layer; Tbx15 is expressed in all of these layers as well as the underlying abdominal muscles. |
T5649 |
1834-1836 |
IN |
denotes |
By |
T5650 |
1870-1876 |
VBN |
denotes |
become |
T5651 |
1837-1842 |
NN |
denotes |
E13.5 |
T5652 |
1842-1844 |
, |
denotes |
, |
T5653 |
1844-1847 |
DT |
denotes |
the |
T5654 |
1859-1865 |
NN |
denotes |
dermis |
T5655 |
1848-1858 |
VBG |
denotes |
developing |
T5656 |
1866-1869 |
VBZ |
denotes |
has |
T5657 |
1954-1963 |
VBN |
denotes |
expressed |
T5658 |
1877-1886 |
JJ |
denotes |
separated |
T5659 |
1887-1891 |
IN |
denotes |
from |
T5660 |
1892-1895 |
DT |
denotes |
the |
T5661 |
1902-1912 |
NN |
denotes |
mesenchyme |
T5662 |
1896-1901 |
JJ |
denotes |
loose |
T5663 |
1913-1915 |
IN |
denotes |
by |
T5664 |
1916-1917 |
DT |
denotes |
a |
T5665 |
1938-1943 |
NN |
denotes |
layer |
T5666 |
1918-1930 |
JJ |
denotes |
subcutaneous |
T5667 |
1931-1937 |
NN |
denotes |
muscle |
T5668 |
1943-1944 |
: |
denotes |
; |
T5669 |
1945-1950 |
NN |
denotes |
Tbx15 |
T5670 |
1951-1953 |
VBZ |
denotes |
is |
T5671 |
1964-1966 |
IN |
denotes |
in |
T5672 |
1967-1970 |
DT |
denotes |
all |
T5673 |
1971-1973 |
IN |
denotes |
of |
T5674 |
1974-1979 |
DT |
denotes |
these |
T5675 |
1980-1986 |
NNS |
denotes |
layers |
T5676 |
1987-1989 |
RB |
denotes |
as |
T5677 |
1995-1997 |
IN |
denotes |
as |
T5678 |
1990-1994 |
RB |
denotes |
well |
T5679 |
1998-2001 |
DT |
denotes |
the |
T5680 |
2023-2030 |
NNS |
denotes |
muscles |
T5681 |
2002-2012 |
VBG |
denotes |
underlying |
T5682 |
2013-2022 |
JJ |
denotes |
abdominal |
T5683 |
2030-2031 |
. |
denotes |
. |
T5684 |
2031-2271 |
sentence |
denotes |
In P3.5 skin, Tbx15 is expressed in both dorsal and ventral skin, most strongly in the condensed upper dermis and developing dermal sheaths of hair follicles; faint expression can also be detected in rare dermal papillae cells (Figure 4B). |
T5685 |
2032-2034 |
IN |
denotes |
In |
T5686 |
2055-2064 |
VBN |
denotes |
expressed |
T5687 |
2035-2039 |
NN |
denotes |
P3.5 |
T5688 |
2040-2044 |
NN |
denotes |
skin |
T5689 |
2044-2046 |
, |
denotes |
, |
T5690 |
2046-2051 |
NN |
denotes |
Tbx15 |
T5691 |
2052-2054 |
VBZ |
denotes |
is |
T5692 |
2220-2228 |
VBN |
denotes |
detected |
T5693 |
2065-2067 |
IN |
denotes |
in |
T5694 |
2068-2072 |
CC |
denotes |
both |
T5695 |
2073-2079 |
JJ |
denotes |
dorsal |
T5696 |
2092-2096 |
NN |
denotes |
skin |
T5697 |
2080-2083 |
CC |
denotes |
and |
T5698 |
2084-2091 |
JJ |
denotes |
ventral |
T5699 |
2096-2098 |
, |
denotes |
, |
T5700 |
2098-2102 |
RBS |
denotes |
most |
T5701 |
2103-2111 |
RB |
denotes |
strongly |
T5702 |
2112-2114 |
IN |
denotes |
in |
T5703 |
2115-2118 |
DT |
denotes |
the |
T5704 |
2135-2141 |
NN |
denotes |
dermis |
T5705 |
2119-2128 |
VBN |
denotes |
condensed |
T5706 |
2129-2134 |
JJ |
denotes |
upper |
T5707 |
2142-2145 |
CC |
denotes |
and |
T5708 |
2146-2156 |
VBG |
denotes |
developing |
T5709 |
2164-2171 |
NNS |
denotes |
sheaths |
T5710 |
2157-2163 |
JJ |
denotes |
dermal |
T5711 |
2172-2174 |
IN |
denotes |
of |
T5712 |
2175-2179 |
NN |
denotes |
hair |
T5713 |
2180-2189 |
NNS |
denotes |
follicles |
T5714 |
2189-2190 |
: |
denotes |
; |
T5715 |
2191-2196 |
JJ |
denotes |
faint |
T5716 |
2197-2207 |
NN |
denotes |
expression |
T5717 |
2208-2211 |
MD |
denotes |
can |
T5718 |
2212-2216 |
RB |
denotes |
also |
T5719 |
2217-2219 |
VB |
denotes |
be |
T5720 |
2229-2231 |
IN |
denotes |
in |
T5721 |
2232-2236 |
JJ |
denotes |
rare |
T5722 |
2253-2258 |
NNS |
denotes |
cells |
T5723 |
2237-2243 |
JJ |
denotes |
dermal |
T5724 |
2244-2252 |
NNS |
denotes |
papillae |
T5725 |
2259-2260 |
-LRB- |
denotes |
( |
T5726 |
2260-2266 |
NN |
denotes |
Figure |
T5727 |
2267-2269 |
CD |
denotes |
4B |
T5728 |
2269-2270 |
-RRB- |
denotes |
) |
T5729 |
2270-2271 |
. |
denotes |
. |
T5730 |
2271-2455 |
sentence |
denotes |
Although the effects of Agouti on pigment-type switching occur during postnatal hair growth, the ventral-specific isoform of Agouti is expressed in developing skin beginning at E11.5. |
T5731 |
2272-2280 |
IN |
denotes |
Although |
T5732 |
2329-2334 |
VBP |
denotes |
occur |
T5733 |
2281-2284 |
DT |
denotes |
the |
T5734 |
2285-2292 |
NNS |
denotes |
effects |
T5735 |
2293-2295 |
IN |
denotes |
of |
T5736 |
2296-2302 |
NN |
denotes |
Agouti |
T5737 |
2303-2305 |
IN |
denotes |
on |
T5738 |
2306-2313 |
NN |
denotes |
pigment |
T5739 |
2314-2318 |
NN |
denotes |
type |
T5740 |
2313-2314 |
HYPH |
denotes |
- |
T5741 |
2319-2328 |
VBG |
denotes |
switching |
T5742 |
2407-2416 |
VBN |
denotes |
expressed |
T5743 |
2335-2341 |
IN |
denotes |
during |
T5744 |
2342-2351 |
JJ |
denotes |
postnatal |
T5745 |
2357-2363 |
NN |
denotes |
growth |
T5746 |
2352-2356 |
NN |
denotes |
hair |
T5747 |
2363-2365 |
, |
denotes |
, |
T5748 |
2365-2368 |
DT |
denotes |
the |
T5749 |
2386-2393 |
NN |
denotes |
isoform |
T5750 |
2369-2376 |
JJ |
denotes |
ventral |
T5751 |
2377-2385 |
JJ |
denotes |
specific |
T5752 |
2376-2377 |
HYPH |
denotes |
- |
T5753 |
2394-2396 |
IN |
denotes |
of |
T5754 |
2397-2403 |
NN |
denotes |
Agouti |
T5755 |
2404-2406 |
VBZ |
denotes |
is |
T5756 |
2417-2419 |
IN |
denotes |
in |
T5757 |
2420-2430 |
VBG |
denotes |
developing |
T5758 |
2431-2435 |
NN |
denotes |
skin |
T5759 |
2436-2445 |
VBG |
denotes |
beginning |
T5760 |
2446-2448 |
IN |
denotes |
at |
T5761 |
2449-2454 |
NN |
denotes |
E11.5 |
T5762 |
2454-2455 |
. |
denotes |
. |
T5763 |
2455-2676 |
sentence |
denotes |
We compared adjacent sections hybridized with probes for Tbx15 and Agouti and observed complementary patterns at E12.5, with expression of Agouti in ventral skin and expression of Tbx15 in dorsal skin (Figure 5A and 5B). |
T5764 |
2456-2458 |
PRP |
denotes |
We |
T5765 |
2459-2467 |
VBD |
denotes |
compared |
T5766 |
2468-2476 |
JJ |
denotes |
adjacent |
T5767 |
2477-2485 |
NNS |
denotes |
sections |
T5768 |
2486-2496 |
VBN |
denotes |
hybridized |
T5769 |
2497-2501 |
IN |
denotes |
with |
T5770 |
2502-2508 |
NNS |
denotes |
probes |
T5771 |
2509-2512 |
IN |
denotes |
for |
T5772 |
2513-2518 |
NN |
denotes |
Tbx15 |
T5773 |
2519-2522 |
CC |
denotes |
and |
T5774 |
2523-2529 |
NN |
denotes |
Agouti |
T5775 |
2530-2533 |
CC |
denotes |
and |
T5776 |
2534-2542 |
VBD |
denotes |
observed |
T5777 |
2543-2556 |
JJ |
denotes |
complementary |
T5778 |
2557-2565 |
NNS |
denotes |
patterns |
T5779 |
2566-2568 |
IN |
denotes |
at |
T5780 |
2569-2574 |
NN |
denotes |
E12.5 |
T5781 |
2574-2576 |
, |
denotes |
, |
T5782 |
2576-2580 |
IN |
denotes |
with |
T5783 |
2581-2591 |
NN |
denotes |
expression |
T5784 |
2592-2594 |
IN |
denotes |
of |
T5785 |
2595-2601 |
NN |
denotes |
Agouti |
T5786 |
2602-2604 |
IN |
denotes |
in |
T5787 |
2605-2612 |
JJ |
denotes |
ventral |
T5788 |
2613-2617 |
NN |
denotes |
skin |
T5789 |
2618-2621 |
CC |
denotes |
and |
T5790 |
2622-2632 |
NN |
denotes |
expression |
T5791 |
2633-2635 |
IN |
denotes |
of |
T5792 |
2636-2641 |
NN |
denotes |
Tbx15 |
T5793 |
2642-2644 |
IN |
denotes |
in |
T5794 |
2645-2651 |
JJ |
denotes |
dorsal |
T5795 |
2652-2656 |
NN |
denotes |
skin |
T5796 |
2657-2658 |
-LRB- |
denotes |
( |
T5797 |
2665-2667 |
CD |
denotes |
5A |
T5798 |
2658-2664 |
NN |
denotes |
Figure |
T5799 |
2668-2671 |
CC |
denotes |
and |
T5800 |
2672-2674 |
CD |
denotes |
5B |
T5801 |
2674-2675 |
-RRB- |
denotes |
) |
T5802 |
2675-2676 |
. |
denotes |
. |
T5803 |
2676-2848 |
sentence |
denotes |
The junction between expression domains is indistinct, and by E14.5, Tbx15 expression extends ventrally and overlaps extensively with Agouti expression (Figure 5C and 5D). |
T5804 |
2677-2680 |
DT |
denotes |
The |
T5805 |
2681-2689 |
NN |
denotes |
junction |
T5806 |
2717-2719 |
VBZ |
denotes |
is |
T5807 |
2690-2697 |
IN |
denotes |
between |
T5808 |
2698-2708 |
NN |
denotes |
expression |
T5809 |
2709-2716 |
NNS |
denotes |
domains |
T5810 |
2720-2730 |
JJ |
denotes |
indistinct |
T5811 |
2730-2732 |
, |
denotes |
, |
T5812 |
2732-2735 |
CC |
denotes |
and |
T5813 |
2736-2738 |
IN |
denotes |
by |
T5814 |
2763-2770 |
VBZ |
denotes |
extends |
T5815 |
2739-2744 |
NN |
denotes |
E14.5 |
T5816 |
2744-2746 |
, |
denotes |
, |
T5817 |
2746-2751 |
NN |
denotes |
Tbx15 |
T5818 |
2752-2762 |
NN |
denotes |
expression |
T5819 |
2771-2780 |
RB |
denotes |
ventrally |
T5820 |
2781-2784 |
CC |
denotes |
and |
T5821 |
2785-2793 |
VBZ |
denotes |
overlaps |
T5822 |
2794-2805 |
RB |
denotes |
extensively |
T5823 |
2806-2810 |
IN |
denotes |
with |
T5824 |
2811-2817 |
NN |
denotes |
Agouti |
T5825 |
2818-2828 |
NN |
denotes |
expression |
T5826 |
2829-2830 |
-LRB- |
denotes |
( |
T5827 |
2837-2839 |
CD |
denotes |
5C |
T5828 |
2830-2836 |
NN |
denotes |
Figure |
T5829 |
2840-2843 |
CC |
denotes |
and |
T5830 |
2844-2846 |
CD |
denotes |
5D |
T5831 |
2846-2847 |
-RRB- |
denotes |
) |
T5832 |
2847-2848 |
. |
denotes |
. |
T5833 |
2848-3503 |
sentence |
denotes |
Figure 5 Embryonic Expression of Tbx15 Compared to Agouti in at/at Mice
(A and C) Tbx15. (B and D) Agouti. At E12.5, expression of Tbx15 in dorsal skin is approximately complementary to that of Agouti in ventral skin. At E14.5, the levels of expression for both genes are lower, but Tbx15 expression has expanded ventrally and overlaps extensively with that of Agouti. In all four panels, arrows mark the approximate ventral limit of Tbx15 and the approximate dorsal limit of Agouti (scale bars = 500 μm). We also examined the effect of deH on expression of Agouti and found no difference between mutant and nonmutant at E12.5 or E13.5 (data not shown). |
T5834 |
3356-3358 |
PRP |
denotes |
We |
T5835 |
3364-3372 |
VBD |
denotes |
examined |
T5836 |
3359-3363 |
RB |
denotes |
also |
T5837 |
3373-3376 |
DT |
denotes |
the |
T5838 |
3377-3383 |
NN |
denotes |
effect |
T5839 |
3384-3386 |
IN |
denotes |
of |
T5840 |
3387-3390 |
NN |
denotes |
deH |
T5841 |
3391-3393 |
IN |
denotes |
on |
T5842 |
3394-3404 |
NN |
denotes |
expression |
T5843 |
3405-3407 |
IN |
denotes |
of |
T5844 |
3408-3414 |
NN |
denotes |
Agouti |
T5845 |
3415-3418 |
CC |
denotes |
and |
T5846 |
3419-3424 |
VBD |
denotes |
found |
T5847 |
3425-3427 |
DT |
denotes |
no |
T5848 |
3428-3438 |
NN |
denotes |
difference |
T5849 |
3439-3446 |
IN |
denotes |
between |
T5850 |
3447-3453 |
JJ |
denotes |
mutant |
T5851 |
3454-3457 |
CC |
denotes |
and |
T5852 |
3458-3467 |
JJ |
denotes |
nonmutant |
T5853 |
3468-3470 |
IN |
denotes |
at |
T5854 |
3471-3476 |
NN |
denotes |
E12.5 |
T5855 |
3477-3479 |
CC |
denotes |
or |
T5856 |
3480-3485 |
NN |
denotes |
E13.5 |
T5857 |
3486-3487 |
-LRB- |
denotes |
( |
T5858 |
3496-3501 |
VBN |
denotes |
shown |
T5859 |
3487-3491 |
NNS |
denotes |
data |
T5860 |
3492-3495 |
RB |
denotes |
not |
T5861 |
3501-3502 |
-RRB- |
denotes |
) |
T5862 |
3502-3503 |
. |
denotes |
. |
T5863 |
3503-3646 |
sentence |
denotes |
However, at E14.5, the normal ventral-to-dorsal gradient of Agouti expression appeared to extend more dorsally in deH/deH embryos (Figure 6A). |
T5864 |
3504-3511 |
RB |
denotes |
However |
T5865 |
3582-3590 |
VBD |
denotes |
appeared |
T5866 |
3511-3513 |
, |
denotes |
, |
T5867 |
3513-3515 |
IN |
denotes |
at |
T5868 |
3516-3521 |
NN |
denotes |
E14.5 |
T5869 |
3521-3523 |
, |
denotes |
, |
T5870 |
3523-3526 |
DT |
denotes |
the |
T5871 |
3552-3560 |
NN |
denotes |
gradient |
T5872 |
3527-3533 |
JJ |
denotes |
normal |
T5873 |
3534-3541 |
JJ |
denotes |
ventral |
T5874 |
3541-3542 |
HYPH |
denotes |
- |
T5875 |
3542-3544 |
IN |
denotes |
to |
T5876 |
3544-3545 |
HYPH |
denotes |
- |
T5877 |
3545-3551 |
JJ |
denotes |
dorsal |
T5878 |
3561-3563 |
IN |
denotes |
of |
T5879 |
3564-3570 |
NN |
denotes |
Agouti |
T5880 |
3571-3581 |
NN |
denotes |
expression |
T5881 |
3591-3593 |
TO |
denotes |
to |
T5882 |
3594-3600 |
VB |
denotes |
extend |
T5883 |
3601-3605 |
RBR |
denotes |
more |
T5884 |
3606-3614 |
RB |
denotes |
dorsally |
T5885 |
3615-3617 |
IN |
denotes |
in |
T5886 |
3618-3621 |
NN |
denotes |
deH |
T5887 |
3622-3625 |
NN |
denotes |
deH |
T5888 |
3621-3622 |
HYPH |
denotes |
/ |
T5889 |
3626-3633 |
NNS |
denotes |
embryos |
T5890 |
3634-3635 |
-LRB- |
denotes |
( |
T5891 |
3635-3641 |
NN |
denotes |
Figure |
T5892 |
3642-3644 |
CD |
denotes |
6A |
T5893 |
3644-3645 |
-RRB- |
denotes |
) |
T5894 |
3645-3646 |
. |
denotes |
. |
T5895 |
3646-3833 |
sentence |
denotes |
In P4.5 skin, expression of Agouti is also extended dorsally in deH/deH animals and is most apparent in the midflank region within the upper dermis and dermal papillae cells (Figure 6B). |
T5896 |
3647-3649 |
IN |
denotes |
In |
T5897 |
3690-3698 |
VBN |
denotes |
extended |
T5898 |
3650-3654 |
NN |
denotes |
P4.5 |
T5899 |
3655-3659 |
NN |
denotes |
skin |
T5900 |
3659-3661 |
, |
denotes |
, |
T5901 |
3661-3671 |
NN |
denotes |
expression |
T5902 |
3672-3674 |
IN |
denotes |
of |
T5903 |
3675-3681 |
NN |
denotes |
Agouti |
T5904 |
3682-3684 |
VBZ |
denotes |
is |
T5905 |
3685-3689 |
RB |
denotes |
also |
T5906 |
3699-3707 |
RB |
denotes |
dorsally |
T5907 |
3708-3710 |
IN |
denotes |
in |
T5908 |
3711-3714 |
NN |
denotes |
deH |
T5909 |
3715-3718 |
NN |
denotes |
deH |
T5910 |
3714-3715 |
HYPH |
denotes |
/ |
T5911 |
3719-3726 |
NNS |
denotes |
animals |
T5912 |
3727-3730 |
CC |
denotes |
and |
T5913 |
3731-3733 |
VBZ |
denotes |
is |
T5914 |
3734-3738 |
RBS |
denotes |
most |
T5915 |
3739-3747 |
JJ |
denotes |
apparent |
T5916 |
3748-3750 |
IN |
denotes |
in |
T5917 |
3751-3754 |
DT |
denotes |
the |
T5918 |
3764-3770 |
NN |
denotes |
region |
T5919 |
3755-3763 |
JJ |
denotes |
midflank |
T5920 |
3771-3777 |
IN |
denotes |
within |
T5921 |
3778-3781 |
DT |
denotes |
the |
T5922 |
3788-3794 |
NN |
denotes |
dermis |
T5923 |
3782-3787 |
JJ |
denotes |
upper |
T5924 |
3795-3798 |
CC |
denotes |
and |
T5925 |
3799-3805 |
JJ |
denotes |
dermal |
T5926 |
3815-3820 |
NNS |
denotes |
cells |
T5927 |
3806-3814 |
NNS |
denotes |
papillae |
T5928 |
3821-3822 |
-LRB- |
denotes |
( |
T5929 |
3822-3828 |
NN |
denotes |
Figure |
T5930 |
3829-3831 |
CD |
denotes |
6B |
T5931 |
3831-3832 |
-RRB- |
denotes |
) |
T5932 |
3832-3833 |
. |
denotes |
. |
T5933 |
3833-4165 |
sentence |
denotes |
Thus, while the pigmentation phenotype of deH/deH mice can be explained, not surprisingly, by dorsal extension of Agouti expression after birth, patterned expression of Tbx15 and Agouti are apparent some 10 days earlier, between E12.5 and E13.5, and the effects of Tbx15 deficiency on expression of Agouti can be detected by E14.5. |
T5934 |
3834-3838 |
RB |
denotes |
Thus |
T5935 |
4020-4023 |
VBP |
denotes |
are |
T5936 |
3838-3840 |
, |
denotes |
, |
T5937 |
3840-3845 |
IN |
denotes |
while |
T5938 |
3896-3905 |
VBN |
denotes |
explained |
T5939 |
3846-3849 |
DT |
denotes |
the |
T5940 |
3863-3872 |
NN |
denotes |
phenotype |
T5941 |
3850-3862 |
NN |
denotes |
pigmentation |
T5942 |
3873-3875 |
IN |
denotes |
of |
T5943 |
3876-3879 |
NN |
denotes |
deH |
T5944 |
3880-3883 |
NN |
denotes |
deH |
T5945 |
3879-3880 |
HYPH |
denotes |
/ |
T5946 |
3884-3888 |
NNS |
denotes |
mice |
T5947 |
3889-3892 |
MD |
denotes |
can |
T5948 |
3893-3895 |
VB |
denotes |
be |
T5949 |
3905-3907 |
, |
denotes |
, |
T5950 |
3907-3910 |
RB |
denotes |
not |
T5951 |
3911-3923 |
RB |
denotes |
surprisingly |
T5952 |
3923-3925 |
, |
denotes |
, |
T5953 |
3925-3927 |
IN |
denotes |
by |
T5954 |
3928-3934 |
JJ |
denotes |
dorsal |
T5955 |
3935-3944 |
NN |
denotes |
extension |
T5956 |
3945-3947 |
IN |
denotes |
of |
T5957 |
3948-3954 |
NN |
denotes |
Agouti |
T5958 |
3955-3965 |
NN |
denotes |
expression |
T5959 |
3966-3971 |
IN |
denotes |
after |
T5960 |
3972-3977 |
NN |
denotes |
birth |
T5961 |
3977-3979 |
, |
denotes |
, |
T5962 |
3979-3988 |
VBN |
denotes |
patterned |
T5963 |
3989-3999 |
NN |
denotes |
expression |
T5964 |
4000-4002 |
IN |
denotes |
of |
T5965 |
4003-4008 |
NN |
denotes |
Tbx15 |
T5966 |
4009-4012 |
CC |
denotes |
and |
T5967 |
4013-4019 |
NN |
denotes |
Agouti |
T5968 |
4024-4032 |
JJ |
denotes |
apparent |
T5969 |
4033-4037 |
DT |
denotes |
some |
T5970 |
4038-4040 |
CD |
denotes |
10 |
T5971 |
4041-4045 |
NNS |
denotes |
days |
T5972 |
4046-4053 |
RBR |
denotes |
earlier |
T5973 |
4053-4055 |
, |
denotes |
, |
T5974 |
4055-4062 |
IN |
denotes |
between |
T5975 |
4063-4068 |
NN |
denotes |
E12.5 |
T5976 |
4069-4072 |
CC |
denotes |
and |
T5977 |
4073-4078 |
NN |
denotes |
E13.5 |
T5978 |
4078-4080 |
, |
denotes |
, |
T5979 |
4080-4083 |
CC |
denotes |
and |
T5980 |
4084-4087 |
DT |
denotes |
the |
T5981 |
4088-4095 |
NNS |
denotes |
effects |
T5982 |
4147-4155 |
VBN |
denotes |
detected |
T5983 |
4096-4098 |
IN |
denotes |
of |
T5984 |
4099-4104 |
NN |
denotes |
Tbx15 |
T5985 |
4105-4115 |
NN |
denotes |
deficiency |
T5986 |
4116-4118 |
IN |
denotes |
on |
T5987 |
4119-4129 |
NN |
denotes |
expression |
T5988 |
4130-4132 |
IN |
denotes |
of |
T5989 |
4133-4139 |
NN |
denotes |
Agouti |
T5990 |
4140-4143 |
MD |
denotes |
can |
T5991 |
4144-4146 |
VB |
denotes |
be |
T5992 |
4156-4158 |
IN |
denotes |
by |
T5993 |
4159-4164 |
NN |
denotes |
E14.5 |
T5994 |
4164-4165 |
. |
denotes |
. |
R3666 |
T5466 |
T5465 |
prep |
of,Expression |
R3667 |
T5467 |
T5466 |
pobj |
Tbx15,of |
R3668 |
T5468 |
T5467 |
cc |
and,Tbx15 |
R3669 |
T5469 |
T5467 |
conj |
Agouti,Tbx15 |
R3670 |
T5471 |
T5472 |
amod |
Previous,studies |
R3671 |
T5472 |
T5473 |
nsubj |
studies,described |
R3672 |
T5474 |
T5472 |
prep |
by,studies |
R3673 |
T5475 |
T5474 |
pobj |
Agulnik,by |
R3674 |
T5476 |
T5477 |
advmod |
et,al. |
R3675 |
T5477 |
T5475 |
advmod |
al.,Agulnik |
R3676 |
T5478 |
T5475 |
punct |
(,Agulnik |
R3677 |
T5479 |
T5475 |
npadvmod |
1998,Agulnik |
R3678 |
T5480 |
T5475 |
punct |
),Agulnik |
R3679 |
T5481 |
T5472 |
acl |
using,studies |
R3680 |
T5482 |
T5483 |
amod |
whole,mount |
R3681 |
T5483 |
T5485 |
nmod |
mount,hybridization |
R3682 |
T5484 |
T5483 |
punct |
-,mount |
R3683 |
T5485 |
T5481 |
dobj |
hybridization,using |
R3684 |
T5486 |
T5487 |
advmod |
in,situ |
R3685 |
T5487 |
T5485 |
amod |
situ,hybridization |
R3686 |
T5488 |
T5473 |
dobj |
expression,described |
R3687 |
T5489 |
T5488 |
prep |
of,expression |
R3688 |
T5490 |
T5489 |
pobj |
Tbx15,of |
R3689 |
T5491 |
T5473 |
prep |
as,described |
R3690 |
T5492 |
T5493 |
advmod |
first,detectable |
R3691 |
T5493 |
T5491 |
pcomp |
detectable,as |
R3692 |
T5494 |
T5493 |
prep |
at,detectable |
R3693 |
T5495 |
T5494 |
pobj |
E9.5,at |
R3694 |
T5496 |
T5493 |
prep |
in,detectable |
R3695 |
T5497 |
T5498 |
det |
the,buds |
R3696 |
T5498 |
T5496 |
pobj |
buds,in |
R3697 |
T5499 |
T5498 |
compound |
limb,buds |
R3698 |
T5500 |
T5493 |
punct |
", ",detectable |
R3699 |
T5501 |
T5493 |
advcl |
progressing,detectable |
R3700 |
T5502 |
T5501 |
prep |
to,progressing |
R3701 |
T5503 |
T5504 |
det |
the,arches |
R3702 |
T5504 |
T5502 |
pobj |
arches,to |
R3703 |
T5505 |
T5504 |
amod |
branchial,arches |
R3704 |
T5506 |
T5504 |
punct |
", ",arches |
R3705 |
T5507 |
T5504 |
conj |
flanks,arches |
R3706 |
T5508 |
T5507 |
punct |
", ",flanks |
R3707 |
T5509 |
T5507 |
cc |
and,flanks |
R3708 |
T5510 |
T5511 |
amod |
craniofacial,regions |
R3709 |
T5511 |
T5507 |
conj |
regions,flanks |
R3710 |
T5512 |
T5501 |
prep |
through,progressing |
R3711 |
T5513 |
T5512 |
pobj |
E12.5,through |
R3712 |
T5514 |
T5473 |
punct |
.,described |
R3713 |
T5516 |
T5517 |
aux |
To,investigate |
R3714 |
T5517 |
T5518 |
advcl |
investigate,hybridized |
R3715 |
T5519 |
T5520 |
det |
this,pattern |
R3716 |
T5520 |
T5517 |
dobj |
pattern,investigate |
R3717 |
T5521 |
T5517 |
prep |
in,investigate |
R3718 |
T5522 |
T5523 |
amod |
more,detail |
R3719 |
T5523 |
T5521 |
pobj |
detail,in |
R3720 |
T5524 |
T5518 |
punct |
", ",hybridized |
R3721 |
T5525 |
T5518 |
nsubj |
we,hybridized |
R3722 |
T5526 |
T5527 |
det |
a,probe |
R3723 |
T5527 |
T5518 |
dobj |
probe,hybridized |
R3724 |
T5528 |
T5527 |
compound |
Tbx15,probe |
R3725 |
T5529 |
T5527 |
compound |
mRNA,probe |
R3726 |
T5530 |
T5518 |
prep |
to,hybridized |
R3727 |
T5531 |
T5532 |
det |
a,series |
R3728 |
T5532 |
T5530 |
pobj |
series,to |
R3729 |
T5533 |
T5532 |
prep |
of,series |
R3730 |
T5534 |
T5535 |
amod |
transverse,sections |
R3731 |
T5535 |
T5533 |
pobj |
sections,of |
R3732 |
T5536 |
T5518 |
prep |
at,hybridized |
R3733 |
T5537 |
T5536 |
pobj |
E12.5,at |
R3734 |
T5538 |
T5518 |
cc |
and,hybridized |
R3735 |
T5539 |
T5518 |
conj |
observed,hybridized |
R3736 |
T5540 |
T5539 |
dobj |
expression,observed |
R3737 |
T5541 |
T5540 |
prep |
in,expression |
R3738 |
T5542 |
T5543 |
amod |
multiple,tissues |
R3739 |
T5543 |
T5541 |
pobj |
tissues,in |
R3740 |
T5544 |
T5543 |
amod |
mesenchymal,tissues |
R3741 |
T5545 |
T5543 |
prep |
of,tissues |
R3742 |
T5546 |
T5547 |
det |
the,head |
R3743 |
T5547 |
T5545 |
pobj |
head,of |
R3744 |
T5548 |
T5547 |
punct |
", ",head |
R3745 |
T5549 |
T5547 |
conj |
trunk,head |
R3746 |
T5550 |
T5549 |
punct |
", ",trunk |
R3747 |
T5551 |
T5549 |
cc |
and,trunk |
R3748 |
T5552 |
T5553 |
amod |
developing,limbs |
R3749 |
T5553 |
T5549 |
conj |
limbs,trunk |
R3750 |
T5554 |
T5555 |
punct |
(,Figure |
R3751 |
T5555 |
T5540 |
parataxis |
Figure,expression |
R3752 |
T5556 |
T5555 |
nummod |
4A,Figure |
R3753 |
T5557 |
T5555 |
punct |
),Figure |
R3754 |
T5558 |
T5540 |
punct |
", ",expression |
R3755 |
T5559 |
T5560 |
dep |
much,is |
R3756 |
T5560 |
T5540 |
relcl |
is,expression |
R3757 |
T5561 |
T5559 |
prep |
of,much |
R3758 |
T5562 |
T5561 |
pobj |
which,of |
R3759 |
T5563 |
T5560 |
acomp |
consistent,is |
R3760 |
T5564 |
T5563 |
prep |
with,consistent |
R3761 |
T5565 |
T5566 |
det |
the,malformations |
R3762 |
T5566 |
T5564 |
pobj |
malformations,with |
R3763 |
T5567 |
T5566 |
nmod |
skull,malformations |
R3764 |
T5568 |
T5567 |
punct |
", ",skull |
R3765 |
T5569 |
T5570 |
amod |
cervical,vertebrae |
R3766 |
T5570 |
T5567 |
conj |
vertebrae,skull |
R3767 |
T5571 |
T5570 |
punct |
", ",vertebrae |
R3768 |
T5572 |
T5570 |
cc |
and,vertebrae |
R3769 |
T5573 |
T5570 |
conj |
limb,vertebrae |
R3770 |
T5574 |
T5566 |
acl |
reported,malformations |
R3771 |
T5575 |
T5574 |
prep |
for,reported |
R3772 |
T5576 |
T5575 |
pobj |
mice,for |
R3773 |
T5577 |
T5576 |
acl |
carrying,mice |
R3774 |
T5578 |
T5579 |
det |
the,allele |
R3775 |
T5579 |
T5577 |
dobj |
allele,carrying |
R3776 |
T5580 |
T5579 |
amod |
original,allele |
R3777 |
T5581 |
T5582 |
amod |
droopy,ear |
R3778 |
T5582 |
T5579 |
compound |
ear,allele |
R3779 |
T5583 |
T5518 |
punct |
.,hybridized |
R3780 |
T5585 |
T5586 |
nsubj |
We,were |
R3781 |
T5587 |
T5588 |
advmod |
particularly,interested |
R3782 |
T5588 |
T5586 |
acomp |
interested,were |
R3783 |
T5589 |
T5588 |
prep |
in,interested |
R3784 |
T5590 |
T5589 |
pcomp |
determining,in |
R3785 |
T5591 |
T5592 |
det |
the,nature |
R3786 |
T5592 |
T5590 |
dobj |
nature,determining |
R3787 |
T5593 |
T5592 |
amod |
exact,nature |
R3788 |
T5594 |
T5592 |
prep |
of,nature |
R3789 |
T5595 |
T5596 |
det |
the,expression |
R3790 |
T5596 |
T5594 |
pobj |
expression,of |
R3791 |
T5597 |
T5596 |
amod |
embryonic,expression |
R3792 |
T5598 |
T5596 |
compound |
flank,expression |
R3793 |
T5599 |
T5592 |
amod |
relative,nature |
R3794 |
T5600 |
T5599 |
prep |
to,relative |
R3795 |
T5601 |
T5602 |
det |
the,phenotype |
R3796 |
T5602 |
T5600 |
pobj |
phenotype,to |
R3797 |
T5603 |
T5602 |
amod |
ventralized,phenotype |
R3798 |
T5604 |
T5602 |
prep |
of,phenotype |
R3799 |
T5605 |
T5606 |
amod |
adult,mice |
R3800 |
T5606 |
T5604 |
pobj |
mice,of |
R3801 |
T5607 |
T5608 |
nmod |
deH,deH |
R3802 |
T5608 |
T5606 |
compound |
deH,mice |
R3803 |
T5609 |
T5608 |
punct |
/,deH |
R3804 |
T5610 |
T5586 |
punct |
.,were |
R3805 |
T5612 |
T5613 |
amod |
Transverse,sections |
R3806 |
T5613 |
T5615 |
nsubj |
sections,reveal |
R3807 |
T5614 |
T5613 |
amod |
abdominal,sections |
R3808 |
T5616 |
T5613 |
prep |
from,sections |
R3809 |
T5617 |
T5618 |
amod |
different,times |
R3810 |
T5618 |
T5616 |
pobj |
times,from |
R3811 |
T5619 |
T5618 |
prep |
during,times |
R3812 |
T5620 |
T5619 |
pobj |
development,during |
R3813 |
T5621 |
T5622 |
det |
a,band |
R3814 |
T5622 |
T5615 |
dobj |
band,reveal |
R3815 |
T5623 |
T5622 |
amod |
dorsolateral,band |
R3816 |
T5624 |
T5622 |
prep |
of,band |
R3817 |
T5625 |
T5624 |
pobj |
expression,of |
R3818 |
T5626 |
T5622 |
prep |
in,band |
R3819 |
T5627 |
T5628 |
det |
the,mesenchyme |
R3820 |
T5628 |
T5626 |
pobj |
mesenchyme,in |
R3821 |
T5629 |
T5628 |
amod |
superficial,mesenchyme |
R3822 |
T5630 |
T5622 |
prep |
at,band |
R3823 |
T5631 |
T5630 |
pobj |
E11.5,at |
R3824 |
T5632 |
T5633 |
dep |
that,broadens |
R3825 |
T5633 |
T5622 |
relcl |
broadens,band |
R3826 |
T5634 |
T5635 |
preconj |
both,dorsally |
R3827 |
T5635 |
T5633 |
advmod |
dorsally,broadens |
R3828 |
T5636 |
T5635 |
cc |
and,dorsally |
R3829 |
T5637 |
T5635 |
conj |
ventrally,dorsally |
R3830 |
T5638 |
T5633 |
prep |
over,broadens |
R3831 |
T5639 |
T5640 |
det |
the,days |
R3832 |
T5640 |
T5638 |
pobj |
days,over |
R3833 |
T5641 |
T5640 |
amod |
next,days |
R3834 |
T5642 |
T5640 |
amod |
several,days |
R3835 |
T5643 |
T5644 |
punct |
(,Figure |
R3836 |
T5644 |
T5633 |
parataxis |
Figure,broadens |
R3837 |
T5645 |
T5644 |
nummod |
4B,Figure |
R3838 |
T5646 |
T5644 |
punct |
),Figure |
R3839 |
T5647 |
T5615 |
punct |
.,reveal |
R3840 |
T5649 |
T5650 |
prep |
By,become |
R3841 |
T5650 |
T5657 |
ccomp |
become,expressed |
R3842 |
T5651 |
T5649 |
pobj |
E13.5,By |
R3843 |
T5652 |
T5650 |
punct |
", ",become |
R3844 |
T5653 |
T5654 |
det |
the,dermis |
R3845 |
T5654 |
T5650 |
nsubj |
dermis,become |
R3846 |
T5655 |
T5654 |
amod |
developing,dermis |
R3847 |
T5656 |
T5650 |
aux |
has,become |
R3848 |
T5658 |
T5650 |
acomp |
separated,become |
R3849 |
T5659 |
T5658 |
prep |
from,separated |
R3850 |
T5660 |
T5661 |
det |
the,mesenchyme |
R3851 |
T5661 |
T5659 |
pobj |
mesenchyme,from |
R3852 |
T5662 |
T5661 |
amod |
loose,mesenchyme |
R3853 |
T5663 |
T5650 |
prep |
by,become |
R3854 |
T5664 |
T5665 |
det |
a,layer |
R3855 |
T5665 |
T5663 |
pobj |
layer,by |
R3856 |
T5666 |
T5665 |
amod |
subcutaneous,layer |
R3857 |
T5667 |
T5665 |
compound |
muscle,layer |
R3858 |
T5668 |
T5657 |
punct |
;,expressed |
R3859 |
T5669 |
T5657 |
nsubjpass |
Tbx15,expressed |
R3860 |
T5670 |
T5657 |
auxpass |
is,expressed |
R3861 |
T5671 |
T5657 |
prep |
in,expressed |
R3862 |
T5672 |
T5671 |
pobj |
all,in |
R3863 |
T5673 |
T5672 |
prep |
of,all |
R3864 |
T5674 |
T5675 |
det |
these,layers |
R3865 |
T5675 |
T5673 |
pobj |
layers,of |
R3866 |
T5676 |
T5677 |
advmod |
as,as |
R3867 |
T5677 |
T5672 |
cc |
as,all |
R3868 |
T5678 |
T5677 |
advmod |
well,as |
R3869 |
T5679 |
T5680 |
det |
the,muscles |
R3870 |
T5680 |
T5672 |
conj |
muscles,all |
R3871 |
T5681 |
T5680 |
amod |
underlying,muscles |
R3872 |
T5682 |
T5680 |
amod |
abdominal,muscles |
R3873 |
T5683 |
T5657 |
punct |
.,expressed |
R3874 |
T5685 |
T5686 |
prep |
In,expressed |
R3875 |
T5686 |
T5692 |
ccomp |
expressed,detected |
R3876 |
T5687 |
T5688 |
compound |
P3.5,skin |
R3877 |
T5688 |
T5685 |
pobj |
skin,In |
R3878 |
T5689 |
T5686 |
punct |
", ",expressed |
R3879 |
T5690 |
T5686 |
nsubjpass |
Tbx15,expressed |
R3880 |
T5691 |
T5686 |
auxpass |
is,expressed |
R3881 |
T5693 |
T5686 |
prep |
in,expressed |
R3882 |
T5694 |
T5695 |
preconj |
both,dorsal |
R3883 |
T5695 |
T5696 |
amod |
dorsal,skin |
R3884 |
T5696 |
T5693 |
pobj |
skin,in |
R3885 |
T5697 |
T5695 |
cc |
and,dorsal |
R3886 |
T5698 |
T5695 |
conj |
ventral,dorsal |
R3887 |
T5699 |
T5686 |
punct |
", ",expressed |
R3888 |
T5700 |
T5701 |
advmod |
most,strongly |
R3889 |
T5701 |
T5686 |
advmod |
strongly,expressed |
R3890 |
T5702 |
T5701 |
prep |
in,strongly |
R3891 |
T5703 |
T5704 |
det |
the,dermis |
R3892 |
T5704 |
T5702 |
pobj |
dermis,in |
R3893 |
T5705 |
T5704 |
amod |
condensed,dermis |
R3894 |
T5706 |
T5704 |
amod |
upper,dermis |
R3895 |
T5707 |
T5704 |
cc |
and,dermis |
R3896 |
T5708 |
T5709 |
amod |
developing,sheaths |
R3897 |
T5709 |
T5704 |
conj |
sheaths,dermis |
R3898 |
T5710 |
T5709 |
amod |
dermal,sheaths |
R3899 |
T5711 |
T5704 |
prep |
of,dermis |
R3900 |
T5712 |
T5713 |
compound |
hair,follicles |
R3901 |
T5713 |
T5711 |
pobj |
follicles,of |
R3902 |
T5714 |
T5692 |
punct |
;,detected |
R3903 |
T5715 |
T5716 |
amod |
faint,expression |
R3904 |
T5716 |
T5692 |
nsubjpass |
expression,detected |
R3905 |
T5717 |
T5692 |
aux |
can,detected |
R3906 |
T5718 |
T5692 |
advmod |
also,detected |
R3907 |
T5719 |
T5692 |
auxpass |
be,detected |
R3908 |
T5720 |
T5692 |
prep |
in,detected |
R3909 |
T5721 |
T5722 |
amod |
rare,cells |
R3910 |
T5722 |
T5720 |
pobj |
cells,in |
R3911 |
T5723 |
T5722 |
amod |
dermal,cells |
R3912 |
T5724 |
T5722 |
compound |
papillae,cells |
R3913 |
T5725 |
T5726 |
punct |
(,Figure |
R3914 |
T5726 |
T5692 |
parataxis |
Figure,detected |
R3915 |
T5727 |
T5726 |
nummod |
4B,Figure |
R3916 |
T5728 |
T5726 |
punct |
),Figure |
R3917 |
T5729 |
T5692 |
punct |
.,detected |
R3918 |
T5731 |
T5732 |
mark |
Although,occur |
R3919 |
T5732 |
T5742 |
advcl |
occur,expressed |
R3920 |
T5733 |
T5734 |
det |
the,effects |
R3921 |
T5734 |
T5732 |
nsubj |
effects,occur |
R3922 |
T5735 |
T5734 |
prep |
of,effects |
R3923 |
T5736 |
T5735 |
pobj |
Agouti,of |
R3924 |
T5737 |
T5734 |
prep |
on,effects |
R3925 |
T5738 |
T5739 |
compound |
pigment,type |
R3926 |
T5739 |
T5737 |
pobj |
type,on |
R3927 |
T5740 |
T5739 |
punct |
-,type |
R3928 |
T5741 |
T5739 |
amod |
switching,type |
R3929 |
T5743 |
T5732 |
prep |
during,occur |
R3930 |
T5744 |
T5745 |
amod |
postnatal,growth |
R3931 |
T5745 |
T5743 |
pobj |
growth,during |
R3932 |
T5746 |
T5745 |
compound |
hair,growth |
R3933 |
T5747 |
T5742 |
punct |
", ",expressed |
R3934 |
T5748 |
T5749 |
det |
the,isoform |
R3935 |
T5749 |
T5742 |
nsubjpass |
isoform,expressed |
R3936 |
T5750 |
T5751 |
amod |
ventral,specific |
R3937 |
T5751 |
T5749 |
amod |
specific,isoform |
R3938 |
T5752 |
T5751 |
punct |
-,specific |
R3939 |
T5753 |
T5749 |
prep |
of,isoform |
R3940 |
T5754 |
T5753 |
pobj |
Agouti,of |
R3941 |
T5755 |
T5742 |
auxpass |
is,expressed |
R3942 |
T5756 |
T5742 |
prep |
in,expressed |
R3943 |
T5757 |
T5758 |
amod |
developing,skin |
R3944 |
T5758 |
T5756 |
pobj |
skin,in |
R3945 |
T5759 |
T5742 |
advcl |
beginning,expressed |
R3946 |
T5760 |
T5759 |
prep |
at,beginning |
R3947 |
T5761 |
T5760 |
pobj |
E11.5,at |
R3948 |
T5762 |
T5742 |
punct |
.,expressed |
R3949 |
T5764 |
T5765 |
nsubj |
We,compared |
R3950 |
T5766 |
T5767 |
amod |
adjacent,sections |
R3951 |
T5767 |
T5765 |
dobj |
sections,compared |
R3952 |
T5768 |
T5767 |
acl |
hybridized,sections |
R3953 |
T5769 |
T5768 |
prep |
with,hybridized |
R3954 |
T5770 |
T5769 |
pobj |
probes,with |
R3955 |
T5771 |
T5770 |
prep |
for,probes |
R3956 |
T5772 |
T5771 |
pobj |
Tbx15,for |
R3957 |
T5773 |
T5772 |
cc |
and,Tbx15 |
R3958 |
T5774 |
T5772 |
conj |
Agouti,Tbx15 |
R3959 |
T5775 |
T5776 |
cc |
and,observed |
R3960 |
T5776 |
T5765 |
dep |
observed,compared |
R3961 |
T5777 |
T5778 |
amod |
complementary,patterns |
R3962 |
T5778 |
T5776 |
dobj |
patterns,observed |
R3963 |
T5779 |
T5776 |
prep |
at,observed |
R3964 |
T5780 |
T5779 |
pobj |
E12.5,at |
R3965 |
T5781 |
T5776 |
punct |
", ",observed |
R3966 |
T5782 |
T5783 |
mark |
with,expression |
R3967 |
T5783 |
T5776 |
advcl |
expression,observed |
R3968 |
T5784 |
T5783 |
prep |
of,expression |
R3969 |
T5785 |
T5784 |
pobj |
Agouti,of |
R3970 |
T5786 |
T5783 |
prep |
in,expression |
R3971 |
T5787 |
T5788 |
amod |
ventral,skin |
R3972 |
T5788 |
T5786 |
pobj |
skin,in |
R3973 |
T5789 |
T5783 |
cc |
and,expression |
R3974 |
T5790 |
T5783 |
conj |
expression,expression |
R3975 |
T5791 |
T5790 |
prep |
of,expression |
R3976 |
T5792 |
T5791 |
pobj |
Tbx15,of |
R3977 |
T5793 |
T5790 |
prep |
in,expression |
R3978 |
T5794 |
T5795 |
amod |
dorsal,skin |
R3979 |
T5795 |
T5793 |
pobj |
skin,in |
R3980 |
T5796 |
T5797 |
punct |
(,5A |
R3981 |
T5797 |
T5776 |
parataxis |
5A,observed |
R3982 |
T5798 |
T5797 |
nmod |
Figure,5A |
R3983 |
T5799 |
T5797 |
cc |
and,5A |
R3984 |
T5800 |
T5797 |
conj |
5B,5A |
R3985 |
T5801 |
T5797 |
punct |
),5A |
R3986 |
T5802 |
T5765 |
punct |
.,compared |
R3987 |
T5804 |
T5805 |
det |
The,junction |
R3988 |
T5805 |
T5806 |
nsubj |
junction,is |
R3989 |
T5807 |
T5805 |
prep |
between,junction |
R3990 |
T5808 |
T5809 |
compound |
expression,domains |
R3991 |
T5809 |
T5807 |
pobj |
domains,between |
R3992 |
T5810 |
T5806 |
acomp |
indistinct,is |
R3993 |
T5811 |
T5806 |
punct |
", ",is |
R3994 |
T5812 |
T5806 |
cc |
and,is |
R3995 |
T5813 |
T5814 |
prep |
by,extends |
R3996 |
T5814 |
T5806 |
conj |
extends,is |
R3997 |
T5815 |
T5813 |
pobj |
E14.5,by |
R3998 |
T5816 |
T5814 |
punct |
", ",extends |
R3999 |
T5817 |
T5818 |
compound |
Tbx15,expression |
R4000 |
T5818 |
T5814 |
nsubj |
expression,extends |
R4001 |
T5819 |
T5814 |
advmod |
ventrally,extends |
R4002 |
T5820 |
T5814 |
cc |
and,extends |
R4003 |
T5821 |
T5814 |
conj |
overlaps,extends |
R4004 |
T5822 |
T5821 |
advmod |
extensively,overlaps |
R4005 |
T5823 |
T5821 |
prep |
with,overlaps |
R4006 |
T5824 |
T5825 |
compound |
Agouti,expression |
R4007 |
T5825 |
T5823 |
pobj |
expression,with |
R4008 |
T5826 |
T5827 |
punct |
(,5C |
R4009 |
T5827 |
T5821 |
parataxis |
5C,overlaps |
R4010 |
T5828 |
T5827 |
nmod |
Figure,5C |
R4011 |
T5829 |
T5827 |
cc |
and,5C |
R4012 |
T5830 |
T5827 |
conj |
5D,5C |
R4013 |
T5831 |
T5827 |
punct |
),5C |
R4014 |
T5832 |
T5814 |
punct |
.,extends |
R4015 |
T5834 |
T5835 |
nsubj |
We,examined |
R4016 |
T5836 |
T5835 |
advmod |
also,examined |
R4017 |
T5837 |
T5838 |
det |
the,effect |
R4018 |
T5838 |
T5835 |
dobj |
effect,examined |
R4019 |
T5839 |
T5838 |
prep |
of,effect |
R4020 |
T5840 |
T5839 |
pobj |
deH,of |
R4021 |
T5841 |
T5838 |
prep |
on,effect |
R4022 |
T5842 |
T5841 |
pobj |
expression,on |
R4023 |
T5843 |
T5842 |
prep |
of,expression |
R4024 |
T5844 |
T5843 |
pobj |
Agouti,of |
R4025 |
T5845 |
T5835 |
cc |
and,examined |
R4026 |
T5846 |
T5835 |
conj |
found,examined |
R4027 |
T5847 |
T5848 |
det |
no,difference |
R4028 |
T5848 |
T5846 |
dobj |
difference,found |
R4029 |
T5849 |
T5848 |
prep |
between,difference |
R4030 |
T5850 |
T5849 |
pobj |
mutant,between |
R4031 |
T5851 |
T5850 |
cc |
and,mutant |
R4032 |
T5852 |
T5850 |
conj |
nonmutant,mutant |
R4033 |
T5853 |
T5846 |
prep |
at,found |
R4034 |
T5854 |
T5853 |
pobj |
E12.5,at |
R4035 |
T5855 |
T5854 |
cc |
or,E12.5 |
R4036 |
T5856 |
T5854 |
conj |
E13.5,E12.5 |
R4037 |
T5857 |
T5858 |
punct |
(,shown |
R4038 |
T5858 |
T5846 |
parataxis |
shown,found |
R4039 |
T5859 |
T5858 |
nsubj |
data,shown |
R4040 |
T5860 |
T5858 |
neg |
not,shown |
R4041 |
T5861 |
T5858 |
punct |
),shown |
R4042 |
T5862 |
T5835 |
punct |
.,examined |
R4043 |
T5864 |
T5865 |
advmod |
However,appeared |
R4044 |
T5866 |
T5865 |
punct |
", ",appeared |
R4045 |
T5867 |
T5865 |
prep |
at,appeared |
R4046 |
T5868 |
T5867 |
pobj |
E14.5,at |
R4047 |
T5869 |
T5865 |
punct |
", ",appeared |
R4048 |
T5870 |
T5871 |
det |
the,gradient |
R4049 |
T5871 |
T5865 |
nsubj |
gradient,appeared |
R4050 |
T5872 |
T5871 |
amod |
normal,gradient |
R4051 |
T5873 |
T5871 |
amod |
ventral,gradient |
R4052 |
T5874 |
T5873 |
punct |
-,ventral |
R4053 |
T5875 |
T5873 |
prep |
to,ventral |
R4054 |
T5876 |
T5875 |
punct |
-,to |
R4055 |
T5877 |
T5875 |
amod |
dorsal,to |
R4056 |
T5878 |
T5871 |
prep |
of,gradient |
R4057 |
T5879 |
T5880 |
compound |
Agouti,expression |
R4058 |
T5880 |
T5878 |
pobj |
expression,of |
R4059 |
T5881 |
T5882 |
aux |
to,extend |
R4060 |
T5882 |
T5865 |
xcomp |
extend,appeared |
R4061 |
T5883 |
T5884 |
advmod |
more,dorsally |
R4062 |
T5884 |
T5882 |
advmod |
dorsally,extend |
R4063 |
T5885 |
T5882 |
prep |
in,extend |
R4064 |
T5886 |
T5887 |
compound |
deH,deH |
R4065 |
T5887 |
T5889 |
compound |
deH,embryos |
R4066 |
T5888 |
T5887 |
punct |
/,deH |
R4067 |
T5889 |
T5885 |
pobj |
embryos,in |
R4068 |
T5890 |
T5891 |
punct |
(,Figure |
R4069 |
T5891 |
T5882 |
parataxis |
Figure,extend |
R4070 |
T5892 |
T5891 |
nummod |
6A,Figure |
R4071 |
T5893 |
T5891 |
punct |
),Figure |
R4072 |
T5894 |
T5865 |
punct |
.,appeared |
R4073 |
T5896 |
T5897 |
prep |
In,extended |
R4074 |
T5898 |
T5899 |
compound |
P4.5,skin |
R4075 |
T5899 |
T5896 |
pobj |
skin,In |
R4076 |
T5900 |
T5897 |
punct |
", ",extended |
R4077 |
T5901 |
T5897 |
nsubjpass |
expression,extended |
R4078 |
T5902 |
T5901 |
prep |
of,expression |
R4079 |
T5903 |
T5902 |
pobj |
Agouti,of |
R4080 |
T5904 |
T5897 |
auxpass |
is,extended |
R4081 |
T5905 |
T5897 |
advmod |
also,extended |
R4082 |
T5906 |
T5897 |
advmod |
dorsally,extended |
R4083 |
T5907 |
T5897 |
prep |
in,extended |
R4084 |
T5908 |
T5909 |
compound |
deH,deH |
R4085 |
T5909 |
T5911 |
compound |
deH,animals |
R4086 |
T5910 |
T5909 |
punct |
/,deH |
R4087 |
T5911 |
T5907 |
pobj |
animals,in |
R4088 |
T5912 |
T5897 |
cc |
and,extended |
R4089 |
T5913 |
T5897 |
conj |
is,extended |
R4090 |
T5914 |
T5915 |
advmod |
most,apparent |
R4091 |
T5915 |
T5913 |
acomp |
apparent,is |
R4092 |
T5916 |
T5913 |
prep |
in,is |
R4093 |
T5917 |
T5918 |
det |
the,region |
R4094 |
T5918 |
T5916 |
pobj |
region,in |
R4095 |
T5919 |
T5918 |
amod |
midflank,region |
R4096 |
T5920 |
T5913 |
prep |
within,is |
R4097 |
T5921 |
T5922 |
det |
the,dermis |
R4098 |
T5922 |
T5920 |
pobj |
dermis,within |
R4099 |
T5923 |
T5922 |
amod |
upper,dermis |
R4100 |
T5924 |
T5922 |
cc |
and,dermis |
R4101 |
T5925 |
T5926 |
amod |
dermal,cells |
R4102 |
T5926 |
T5922 |
conj |
cells,dermis |
R4103 |
T5927 |
T5926 |
compound |
papillae,cells |
R4104 |
T5928 |
T5929 |
punct |
(,Figure |
R4105 |
T5929 |
T5913 |
parataxis |
Figure,is |
R4106 |
T5930 |
T5929 |
nummod |
6B,Figure |
R4107 |
T5931 |
T5929 |
punct |
),Figure |
R4108 |
T5932 |
T5897 |
punct |
.,extended |
R4109 |
T5934 |
T5935 |
advmod |
Thus,are |
R4110 |
T5936 |
T5935 |
punct |
", ",are |
R4111 |
T5937 |
T5938 |
mark |
while,explained |
R4113 |
T5939 |
T5940 |
det |
the,phenotype |
R4114 |
T5940 |
T5938 |
nsubjpass |
phenotype,explained |
R4115 |
T5941 |
T5940 |
compound |
pigmentation,phenotype |
R4116 |
T5942 |
T5940 |
prep |
of,phenotype |
R4117 |
T5943 |
T5944 |
compound |
deH,deH |
R4118 |
T5944 |
T5946 |
compound |
deH,mice |
R4119 |
T5945 |
T5944 |
punct |
/,deH |
R4120 |
T5946 |
T5942 |
pobj |
mice,of |
R4121 |
T5947 |
T5938 |
aux |
can,explained |
R4122 |
T5948 |
T5938 |
auxpass |
be,explained |
R4123 |
T5949 |
T5938 |
punct |
", ",explained |
R4124 |
T5950 |
T5951 |
neg |
not,surprisingly |
R4125 |
T5951 |
T5938 |
advmod |
surprisingly,explained |
R4126 |
T5952 |
T5938 |
punct |
", ",explained |
R4127 |
T5953 |
T5938 |
agent |
by,explained |
R4128 |
T5954 |
T5955 |
amod |
dorsal,extension |
R4129 |
T5955 |
T5953 |
pobj |
extension,by |
R4130 |
T5956 |
T5955 |
prep |
of,extension |
R4131 |
T5957 |
T5958 |
compound |
Agouti,expression |
R4132 |
T5958 |
T5956 |
pobj |
expression,of |
R4133 |
T5959 |
T5955 |
prep |
after,extension |
R4134 |
T5960 |
T5959 |
pobj |
birth,after |
R4135 |
T5961 |
T5935 |
punct |
", ",are |
R4136 |
T5962 |
T5963 |
amod |
patterned,expression |
R4137 |
T5963 |
T5935 |
nsubj |
expression,are |
R4138 |
T5964 |
T5963 |
prep |
of,expression |
R4139 |
T5965 |
T5964 |
pobj |
Tbx15,of |
R4140 |
T5966 |
T5965 |
cc |
and,Tbx15 |
R4141 |
T5967 |
T5965 |
conj |
Agouti,Tbx15 |
R4142 |
T5968 |
T5935 |
acomp |
apparent,are |
R4143 |
T5969 |
T5970 |
quantmod |
some,10 |
R4144 |
T5970 |
T5971 |
nummod |
10,days |
R4145 |
T5971 |
T5972 |
npadvmod |
days,earlier |
R4146 |
T5972 |
T5935 |
advmod |
earlier,are |
R4147 |
T5973 |
T5935 |
punct |
", ",are |
R4148 |
T5974 |
T5935 |
prep |
between,are |
R4149 |
T5975 |
T5974 |
pobj |
E12.5,between |
R4150 |
T5976 |
T5975 |
cc |
and,E12.5 |
R4151 |
T5977 |
T5975 |
conj |
E13.5,E12.5 |
R4152 |
T5978 |
T5935 |
punct |
", ",are |
R4153 |
T5979 |
T5935 |
cc |
and,are |
R4154 |
T5980 |
T5981 |
det |
the,effects |
R4155 |
T5981 |
T5982 |
nsubjpass |
effects,detected |
R4156 |
T5982 |
T5935 |
conj |
detected,are |
R4157 |
T5983 |
T5981 |
prep |
of,effects |
R4158 |
T5984 |
T5985 |
compound |
Tbx15,deficiency |
R4159 |
T5985 |
T5983 |
pobj |
deficiency,of |
R4160 |
T5986 |
T5981 |
prep |
on,effects |
R4161 |
T5987 |
T5986 |
pobj |
expression,on |
R4162 |
T5988 |
T5987 |
prep |
of,expression |
R4163 |
T5989 |
T5988 |
pobj |
Agouti,of |
R4164 |
T5990 |
T5982 |
aux |
can,detected |
R4165 |
T5991 |
T5982 |
auxpass |
be,detected |
R4166 |
T5992 |
T5982 |
prep |
by,detected |
R4167 |
T5993 |
T5992 |
pobj |
E14.5,by |
R4168 |
T5994 |
T5935 |
punct |
.,are |
R4112 |
T5938 |
T5935 |
advcl |
explained,are |