PMC:314463 / 21142-22066 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/314463","sourcedb":"PMC","sourceid":"314463","source_url":"http://www.ncbi.nlm.nih.gov/pmc/314463","text":"Molecular Genetics of deH and Tbx15\n(A) Genetic and physical map, as described in the text. Markers M1 to M3 are SSCP markers generated from a BAC contig of the region; marker M4 is STS 16.MMHAP32FLF1 and was also used as an SSCP marker. M2 and M3, which flank the Tbx15 and M6pr-ps on the UCSC genome browser map and lie 634 kb apart, were nonrecombinant with deH in 2340 meioses.\n(B) The deH mutation is a deletion that starts in Tbx15 intron 1 and ends in the M6pr-ps.\n(C) Sequence of deletion breakpoints.\n(D) Diagram of Tbx15LacZ allele constructed by gene targeting. As described in the text, this allele is predicted to give rise to a protein truncated after approximately 154 codons and is lacking critical residues of the T box. Heterozygotes for the targeted allele exhibit normal size, morphology, and hair-color patterns, but homozygotes and Tbx15LacZ/deH compound heterozygotes are identical to deH homozygotes.","divisions":[{"label":"title","span":{"begin":0,"end":35}},{"label":"p","span":{"begin":36,"end":381}},{"label":"p","span":{"begin":382,"end":471}},{"label":"p","span":{"begin":472,"end":509}}],"tracks":[]}