Id |
Subject |
Object |
Predicate |
Lexical cue |
T4613 |
0-10 |
JJ |
denotes |
Positional |
T4614 |
11-18 |
NN |
denotes |
Cloning |
T4615 |
19-21 |
IN |
denotes |
of |
T4616 |
22-25 |
NN |
denotes |
deH |
T4617 |
25-311 |
sentence |
denotes |
As a visible marker, early linkage studies with the original droopy ear allele or the deH allele identified a map position in the middle of Chromosome 3, distal to matted and proximal to Varitint-waddler (Carter and Falconer 1951; Curry 1959; Lane and Eicher 1979; Holmes et al. 1981). |
T4618 |
26-28 |
IN |
denotes |
As |
T4619 |
123-133 |
VBD |
denotes |
identified |
T4620 |
29-30 |
DT |
denotes |
a |
T4621 |
39-45 |
NN |
denotes |
marker |
T4622 |
31-38 |
JJ |
denotes |
visible |
T4623 |
45-47 |
, |
denotes |
, |
T4624 |
47-52 |
JJ |
denotes |
early |
T4625 |
61-68 |
NNS |
denotes |
studies |
T4626 |
53-60 |
NN |
denotes |
linkage |
T4627 |
69-73 |
IN |
denotes |
with |
T4628 |
74-77 |
DT |
denotes |
the |
T4629 |
98-104 |
NN |
denotes |
allele |
T4630 |
78-86 |
JJ |
denotes |
original |
T4631 |
87-93 |
JJ |
denotes |
droopy |
T4632 |
94-97 |
NN |
denotes |
ear |
T4633 |
105-107 |
CC |
denotes |
or |
T4634 |
108-111 |
DT |
denotes |
the |
T4635 |
116-122 |
NN |
denotes |
allele |
T4636 |
112-115 |
NN |
denotes |
deH |
T4637 |
134-135 |
DT |
denotes |
a |
T4638 |
140-148 |
NN |
denotes |
position |
T4639 |
136-139 |
NN |
denotes |
map |
T4640 |
149-151 |
IN |
denotes |
in |
T4641 |
152-155 |
DT |
denotes |
the |
T4642 |
156-162 |
NN |
denotes |
middle |
T4643 |
163-165 |
IN |
denotes |
of |
T4644 |
166-176 |
NN |
denotes |
Chromosome |
T4645 |
177-178 |
CD |
denotes |
3 |
T4646 |
178-180 |
, |
denotes |
, |
T4647 |
180-186 |
JJ |
denotes |
distal |
T4648 |
187-189 |
IN |
denotes |
to |
T4649 |
190-196 |
JJ |
denotes |
matted |
T4650 |
197-200 |
CC |
denotes |
and |
T4651 |
201-209 |
JJ |
denotes |
proximal |
T4652 |
210-212 |
IN |
denotes |
to |
T4653 |
213-221 |
NNP |
denotes |
Varitint |
T4654 |
222-229 |
NNP |
denotes |
waddler |
T4655 |
221-222 |
HYPH |
denotes |
- |
T4656 |
230-231 |
-LRB- |
denotes |
( |
T4657 |
231-237 |
NNP |
denotes |
Carter |
T4658 |
238-241 |
CC |
denotes |
and |
T4659 |
242-250 |
NNP |
denotes |
Falconer |
T4660 |
251-255 |
CD |
denotes |
1951 |
T4661 |
255-256 |
: |
denotes |
; |
T4662 |
257-262 |
NNP |
denotes |
Curry |
T4663 |
263-267 |
CD |
denotes |
1959 |
T4664 |
267-268 |
: |
denotes |
; |
T4665 |
269-273 |
NNP |
denotes |
Lane |
T4666 |
274-277 |
CC |
denotes |
and |
T4667 |
278-284 |
NNP |
denotes |
Eicher |
T4668 |
285-289 |
CD |
denotes |
1979 |
T4669 |
289-290 |
: |
denotes |
; |
T4670 |
291-297 |
NNP |
denotes |
Holmes |
T4671 |
298-300 |
FW |
denotes |
et |
T4672 |
301-304 |
FW |
denotes |
al. |
T4673 |
305-309 |
CD |
denotes |
1981 |
T4674 |
309-310 |
-RRB- |
denotes |
) |
T4675 |
310-311 |
. |
denotes |
. |
T4676 |
311-610 |
sentence |
denotes |
We used an F2 intercross with CAST/Ei mice to localize deH to a 0.1 cM interval between D3Mit213 and 16.MMHAP32FLF1, which was refined by development of a bacterial artificial chromosome (BAC) contig and additional markers to a 1.4 Mb region that contained eight genes, including Tbx15 (Figure 3A). |
T4677 |
312-314 |
PRP |
denotes |
We |
T4678 |
315-319 |
VBD |
denotes |
used |
T4679 |
320-322 |
DT |
denotes |
an |
T4680 |
326-336 |
NN |
denotes |
intercross |
T4681 |
323-325 |
NN |
denotes |
F2 |
T4682 |
337-341 |
IN |
denotes |
with |
T4683 |
342-346 |
NN |
denotes |
CAST |
T4684 |
347-349 |
NN |
denotes |
Ei |
T4685 |
346-347 |
HYPH |
denotes |
/ |
T4686 |
350-354 |
NNS |
denotes |
mice |
T4687 |
355-357 |
TO |
denotes |
to |
T4688 |
358-366 |
VB |
denotes |
localize |
T4689 |
367-370 |
NN |
denotes |
deH |
T4690 |
371-373 |
IN |
denotes |
to |
T4691 |
374-375 |
DT |
denotes |
a |
T4692 |
383-391 |
NN |
denotes |
interval |
T4693 |
376-379 |
CD |
denotes |
0.1 |
T4694 |
380-382 |
NN |
denotes |
cM |
T4695 |
392-399 |
IN |
denotes |
between |
T4696 |
400-408 |
NN |
denotes |
D3Mit213 |
T4697 |
409-412 |
CC |
denotes |
and |
T4698 |
413-427 |
NN |
denotes |
16.MMHAP32FLF1 |
T4699 |
427-429 |
, |
denotes |
, |
T4700 |
429-434 |
WDT |
denotes |
which |
T4701 |
439-446 |
VBN |
denotes |
refined |
T4702 |
435-438 |
VBD |
denotes |
was |
T4703 |
447-449 |
IN |
denotes |
by |
T4704 |
450-461 |
NN |
denotes |
development |
T4705 |
462-464 |
IN |
denotes |
of |
T4706 |
465-466 |
DT |
denotes |
a |
T4707 |
505-511 |
NN |
denotes |
contig |
T4708 |
467-476 |
JJ |
denotes |
bacterial |
T4709 |
488-498 |
NN |
denotes |
chromosome |
T4710 |
477-487 |
JJ |
denotes |
artificial |
T4711 |
499-500 |
-LRB- |
denotes |
( |
T4712 |
500-503 |
NN |
denotes |
BAC |
T4713 |
503-504 |
-RRB- |
denotes |
) |
T4714 |
512-515 |
CC |
denotes |
and |
T4715 |
516-526 |
JJ |
denotes |
additional |
T4716 |
527-534 |
NNS |
denotes |
markers |
T4717 |
535-537 |
IN |
denotes |
to |
T4718 |
538-539 |
DT |
denotes |
a |
T4719 |
547-553 |
NN |
denotes |
region |
T4720 |
540-543 |
CD |
denotes |
1.4 |
T4721 |
544-546 |
NN |
denotes |
Mb |
T4722 |
554-558 |
WDT |
denotes |
that |
T4723 |
559-568 |
VBD |
denotes |
contained |
T4724 |
569-574 |
CD |
denotes |
eight |
T4725 |
575-580 |
NNS |
denotes |
genes |
T4726 |
580-582 |
, |
denotes |
, |
T4727 |
582-591 |
VBG |
denotes |
including |
T4728 |
592-597 |
NN |
denotes |
Tbx15 |
T4729 |
598-599 |
-LRB- |
denotes |
( |
T4730 |
599-605 |
NN |
denotes |
Figure |
T4731 |
606-608 |
CD |
denotes |
3A |
T4732 |
608-609 |
-RRB- |
denotes |
) |
T4733 |
609-610 |
. |
denotes |
. |
T4734 |
610-833 |
sentence |
denotes |
We considered Tbx15 as an excellent candidate for the skeletal abnormalities caused by deH, based on studies by Agulnik et al. (1998), who described its embryonic expression in the craniofacial region and developing limbs. |
T4735 |
611-613 |
PRP |
denotes |
We |
T4736 |
614-624 |
VBD |
denotes |
considered |
T4737 |
625-630 |
NN |
denotes |
Tbx15 |
T4738 |
631-633 |
IN |
denotes |
as |
T4739 |
634-636 |
DT |
denotes |
an |
T4740 |
647-656 |
NN |
denotes |
candidate |
T4741 |
637-646 |
JJ |
denotes |
excellent |
T4742 |
657-660 |
IN |
denotes |
for |
T4743 |
661-664 |
DT |
denotes |
the |
T4744 |
674-687 |
NNS |
denotes |
abnormalities |
T4745 |
665-673 |
JJ |
denotes |
skeletal |
T4746 |
688-694 |
VBN |
denotes |
caused |
T4747 |
695-697 |
IN |
denotes |
by |
T4748 |
698-701 |
NN |
denotes |
deH |
T4749 |
701-703 |
, |
denotes |
, |
T4750 |
703-708 |
VBN |
denotes |
based |
T4751 |
709-711 |
IN |
denotes |
on |
T4752 |
712-719 |
NNS |
denotes |
studies |
T4753 |
720-722 |
IN |
denotes |
by |
T4754 |
723-730 |
NNP |
denotes |
Agulnik |
T4755 |
731-733 |
FW |
denotes |
et |
T4756 |
734-737 |
FW |
denotes |
al. |
T4757 |
738-739 |
-LRB- |
denotes |
( |
T4758 |
739-743 |
CD |
denotes |
1998 |
T4759 |
743-744 |
-RRB- |
denotes |
) |
T4760 |
744-746 |
, |
denotes |
, |
T4761 |
746-749 |
WP |
denotes |
who |
T4762 |
750-759 |
VBD |
denotes |
described |
T4763 |
760-763 |
PRP$ |
denotes |
its |
T4764 |
774-784 |
NN |
denotes |
expression |
T4765 |
764-773 |
JJ |
denotes |
embryonic |
T4766 |
785-787 |
IN |
denotes |
in |
T4767 |
788-791 |
DT |
denotes |
the |
T4768 |
805-811 |
NN |
denotes |
region |
T4769 |
792-804 |
JJ |
denotes |
craniofacial |
T4770 |
812-815 |
CC |
denotes |
and |
T4771 |
816-826 |
VBG |
denotes |
developing |
T4772 |
827-832 |
NNS |
denotes |
limbs |
T4773 |
832-833 |
. |
denotes |
. |
T4774 |
833-1968 |
sentence |
denotes |
Figure 3 Molecular Genetics of deH and Tbx15
(A) Genetic and physical map, as described in the text. Markers M1 to M3 are SSCP markers generated from a BAC contig of the region; marker M4 is STS 16.MMHAP32FLF1 and was also used as an SSCP marker. M2 and M3, which flank the Tbx15 and M6pr-ps on the UCSC genome browser map and lie 634 kb apart, were nonrecombinant with deH in 2340 meioses.
(B) The deH mutation is a deletion that starts in Tbx15 intron 1 and ends in the M6pr-ps.
(C) Sequence of deletion breakpoints.
(D) Diagram of Tbx15LacZ allele constructed by gene targeting. As described in the text, this allele is predicted to give rise to a protein truncated after approximately 154 codons and is lacking critical residues of the T box. Heterozygotes for the targeted allele exhibit normal size, morphology, and hair-color patterns, but homozygotes and Tbx15LacZ/deH compound heterozygotes are identical to deH homozygotes. Using sequence information from Agulnik et al. (1998) and the partially completed mouse genome sequence, we found that portions of several Tbx15 exons could not be amplified from deH/deH genomic DNA. |
T4775 |
1769-1774 |
VBG |
denotes |
Using |
T4776 |
1877-1882 |
VBD |
denotes |
found |
T4777 |
1775-1783 |
NN |
denotes |
sequence |
T4778 |
1784-1795 |
NN |
denotes |
information |
T4779 |
1796-1800 |
IN |
denotes |
from |
T4780 |
1801-1808 |
NNP |
denotes |
Agulnik |
T4781 |
1809-1811 |
FW |
denotes |
et |
T4782 |
1812-1815 |
FW |
denotes |
al. |
T4783 |
1816-1817 |
-LRB- |
denotes |
( |
T4784 |
1817-1821 |
CD |
denotes |
1998 |
T4785 |
1821-1822 |
-RRB- |
denotes |
) |
T4786 |
1823-1826 |
CC |
denotes |
and |
T4787 |
1827-1830 |
DT |
denotes |
the |
T4788 |
1864-1872 |
NN |
denotes |
sequence |
T4789 |
1831-1840 |
RB |
denotes |
partially |
T4790 |
1841-1850 |
VBN |
denotes |
completed |
T4791 |
1851-1856 |
NN |
denotes |
mouse |
T4792 |
1857-1863 |
NN |
denotes |
genome |
T4793 |
1872-1874 |
, |
denotes |
, |
T4794 |
1874-1876 |
PRP |
denotes |
we |
T4795 |
1883-1887 |
IN |
denotes |
that |
T4796 |
1933-1942 |
VBN |
denotes |
amplified |
T4797 |
1888-1896 |
NNS |
denotes |
portions |
T4798 |
1897-1899 |
IN |
denotes |
of |
T4799 |
1900-1907 |
JJ |
denotes |
several |
T4800 |
1914-1919 |
NNS |
denotes |
exons |
T4801 |
1908-1913 |
NN |
denotes |
Tbx15 |
T4802 |
1920-1925 |
MD |
denotes |
could |
T4803 |
1926-1929 |
RB |
denotes |
not |
T4804 |
1930-1932 |
VB |
denotes |
be |
T4805 |
1943-1947 |
IN |
denotes |
from |
T4806 |
1948-1951 |
NN |
denotes |
deH |
T4807 |
1952-1955 |
NN |
denotes |
deH |
T4808 |
1951-1952 |
HYPH |
denotes |
/ |
T4809 |
1964-1967 |
NN |
denotes |
DNA |
T4810 |
1956-1963 |
JJ |
denotes |
genomic |
T4811 |
1967-1968 |
. |
denotes |
. |
T4812 |
1968-2181 |
sentence |
denotes |
The same gene was initially referred to as Tbx8 (Wattler et al. 1998) and then later renamed Tbx14, but is currently referred to in several vertebrate genomes as Tbx15 (Agulnik et al. 1998; Begemann et al. 2002). |
T4813 |
1969-1972 |
DT |
denotes |
The |
T4814 |
1978-1982 |
NN |
denotes |
gene |
T4815 |
1973-1977 |
JJ |
denotes |
same |
T4816 |
1997-2005 |
VBN |
denotes |
referred |
T4817 |
1983-1986 |
VBD |
denotes |
was |
T4818 |
1987-1996 |
RB |
denotes |
initially |
T4819 |
2006-2008 |
IN |
denotes |
to |
T4820 |
2009-2011 |
IN |
denotes |
as |
T4821 |
2012-2016 |
NN |
denotes |
Tbx8 |
T4822 |
2017-2018 |
-LRB- |
denotes |
( |
T4823 |
2018-2025 |
NNP |
denotes |
Wattler |
T4824 |
2026-2028 |
FW |
denotes |
et |
T4825 |
2029-2032 |
FW |
denotes |
al. |
T4826 |
2033-2037 |
CD |
denotes |
1998 |
T4827 |
2037-2038 |
-RRB- |
denotes |
) |
T4828 |
2039-2042 |
CC |
denotes |
and |
T4829 |
2043-2047 |
RB |
denotes |
then |
T4830 |
2054-2061 |
VBN |
denotes |
renamed |
T4831 |
2048-2053 |
RB |
denotes |
later |
T4832 |
2062-2067 |
NN |
denotes |
Tbx14 |
T4833 |
2067-2069 |
, |
denotes |
, |
T4834 |
2069-2072 |
CC |
denotes |
but |
T4835 |
2073-2075 |
VBZ |
denotes |
is |
T4836 |
2086-2094 |
VBN |
denotes |
referred |
T4837 |
2076-2085 |
RB |
denotes |
currently |
T4838 |
2095-2097 |
IN |
denotes |
to |
T4839 |
2098-2100 |
IN |
denotes |
in |
T4840 |
2101-2108 |
JJ |
denotes |
several |
T4841 |
2120-2127 |
NNS |
denotes |
genomes |
T4842 |
2109-2119 |
NN |
denotes |
vertebrate |
T4843 |
2128-2130 |
IN |
denotes |
as |
T4844 |
2131-2136 |
NN |
denotes |
Tbx15 |
T4845 |
2137-2138 |
-LRB- |
denotes |
( |
T4846 |
2138-2145 |
NNP |
denotes |
Agulnik |
T4847 |
2146-2148 |
FW |
denotes |
et |
T4848 |
2149-2152 |
FW |
denotes |
al. |
T4849 |
2153-2157 |
CD |
denotes |
1998 |
T4850 |
2157-2158 |
: |
denotes |
; |
T4851 |
2159-2167 |
NNP |
denotes |
Begemann |
T4852 |
2168-2170 |
FW |
denotes |
et |
T4853 |
2171-2174 |
FW |
denotes |
al. |
T4854 |
2175-2179 |
CD |
denotes |
2002 |
T4855 |
2179-2180 |
-RRB- |
denotes |
) |
T4856 |
2180-2181 |
. |
denotes |
. |
T4857 |
2181-2531 |
sentence |
denotes |
By comparing the sequence of a 1.3 kb junction fragment amplified from deH/deH genomic DNA to publicly available mouse genome sequence, we identified a 216 kb deletion that extends from Tbx15 intron 1 to 148 kb downstream of the polyadenylation sequence in a region annotated as a mannose-6-phosphate receptor pseudogene, M6pr-ps (Figure 3B and 3C). |
T4858 |
2182-2184 |
IN |
denotes |
By |
T4859 |
2321-2331 |
VBD |
denotes |
identified |
T4860 |
2185-2194 |
VBG |
denotes |
comparing |
T4861 |
2195-2198 |
DT |
denotes |
the |
T4862 |
2199-2207 |
NN |
denotes |
sequence |
T4863 |
2208-2210 |
IN |
denotes |
of |
T4864 |
2211-2212 |
DT |
denotes |
a |
T4865 |
2229-2237 |
NN |
denotes |
fragment |
T4866 |
2213-2216 |
CD |
denotes |
1.3 |
T4867 |
2217-2219 |
NN |
denotes |
kb |
T4868 |
2220-2228 |
NN |
denotes |
junction |
T4869 |
2238-2247 |
VBN |
denotes |
amplified |
T4870 |
2248-2252 |
IN |
denotes |
from |
T4871 |
2253-2256 |
NN |
denotes |
deH |
T4872 |
2257-2260 |
NN |
denotes |
deH |
T4873 |
2256-2257 |
HYPH |
denotes |
/ |
T4874 |
2269-2272 |
NN |
denotes |
DNA |
T4875 |
2261-2268 |
JJ |
denotes |
genomic |
T4876 |
2273-2275 |
IN |
denotes |
to |
T4877 |
2276-2284 |
RB |
denotes |
publicly |
T4878 |
2285-2294 |
JJ |
denotes |
available |
T4879 |
2308-2316 |
NN |
denotes |
sequence |
T4880 |
2295-2300 |
NN |
denotes |
mouse |
T4881 |
2301-2307 |
NN |
denotes |
genome |
T4882 |
2316-2318 |
, |
denotes |
, |
T4883 |
2318-2320 |
PRP |
denotes |
we |
T4884 |
2332-2333 |
DT |
denotes |
a |
T4885 |
2341-2349 |
NN |
denotes |
deletion |
T4886 |
2334-2337 |
CD |
denotes |
216 |
T4887 |
2338-2340 |
NN |
denotes |
kb |
T4888 |
2350-2354 |
WDT |
denotes |
that |
T4889 |
2355-2362 |
VBZ |
denotes |
extends |
T4890 |
2363-2367 |
IN |
denotes |
from |
T4891 |
2368-2373 |
NN |
denotes |
Tbx15 |
T4892 |
2374-2380 |
NN |
denotes |
intron |
T4893 |
2381-2382 |
CD |
denotes |
1 |
T4894 |
2383-2385 |
IN |
denotes |
to |
T4895 |
2386-2389 |
CD |
denotes |
148 |
T4896 |
2390-2392 |
NN |
denotes |
kb |
T4897 |
2393-2403 |
RB |
denotes |
downstream |
T4898 |
2404-2406 |
IN |
denotes |
of |
T4899 |
2407-2410 |
DT |
denotes |
the |
T4900 |
2427-2435 |
NN |
denotes |
sequence |
T4901 |
2411-2426 |
NN |
denotes |
polyadenylation |
T4902 |
2436-2438 |
IN |
denotes |
in |
T4903 |
2439-2440 |
DT |
denotes |
a |
T4904 |
2441-2447 |
NN |
denotes |
region |
T4905 |
2448-2457 |
VBN |
denotes |
annotated |
T4906 |
2458-2460 |
IN |
denotes |
as |
T4907 |
2461-2462 |
DT |
denotes |
a |
T4908 |
2492-2502 |
NN |
denotes |
pseudogene |
T4909 |
2463-2470 |
NN |
denotes |
mannose |
T4910 |
2473-2482 |
NN |
denotes |
phosphate |
T4911 |
2470-2471 |
HYPH |
denotes |
- |
T4912 |
2471-2472 |
CD |
denotes |
6 |
T4913 |
2472-2473 |
HYPH |
denotes |
- |
T4914 |
2483-2491 |
NN |
denotes |
receptor |
T4915 |
2502-2504 |
, |
denotes |
, |
T4916 |
2504-2508 |
NN |
denotes |
M6pr |
T4917 |
2509-2511 |
NN |
denotes |
ps |
T4918 |
2508-2509 |
HYPH |
denotes |
- |
T4919 |
2512-2513 |
-LRB- |
denotes |
( |
T4920 |
2520-2522 |
CD |
denotes |
3B |
T4921 |
2513-2519 |
NN |
denotes |
Figure |
T4922 |
2523-2526 |
CC |
denotes |
and |
T4923 |
2527-2529 |
CD |
denotes |
3C |
T4924 |
2529-2530 |
-RRB- |
denotes |
) |
T4925 |
2530-2531 |
. |
denotes |
. |
T4926 |
2531-2553 |
sentence |
denotes |
(Ludwig et al. 1992). |
T4927 |
2532-2533 |
-LRB- |
denotes |
( |
T4928 |
2533-2539 |
NNP |
denotes |
Ludwig |
T4929 |
2540-2542 |
FW |
denotes |
et |
T4930 |
2543-2546 |
FW |
denotes |
al. |
T4931 |
2547-2551 |
CD |
denotes |
1992 |
T4932 |
2551-2552 |
-RRB- |
denotes |
) |
T4933 |
2552-2553 |
. |
denotes |
. |
T4934 |
2553-2665 |
sentence |
denotes |
By Northern blot analysis, we identified a fusion transcript produced from the deH chromosome (data not shown). |
T4935 |
2554-2556 |
IN |
denotes |
By |
T4936 |
2584-2594 |
VBD |
denotes |
identified |
T4937 |
2557-2565 |
NNP |
denotes |
Northern |
T4938 |
2566-2570 |
NN |
denotes |
blot |
T4939 |
2571-2579 |
NN |
denotes |
analysis |
T4940 |
2579-2581 |
, |
denotes |
, |
T4941 |
2581-2583 |
PRP |
denotes |
we |
T4942 |
2595-2596 |
DT |
denotes |
a |
T4943 |
2604-2614 |
NN |
denotes |
transcript |
T4944 |
2597-2603 |
NN |
denotes |
fusion |
T4945 |
2615-2623 |
VBN |
denotes |
produced |
T4946 |
2624-2628 |
IN |
denotes |
from |
T4947 |
2629-2632 |
DT |
denotes |
the |
T4948 |
2637-2647 |
NN |
denotes |
chromosome |
T4949 |
2633-2636 |
NN |
denotes |
deH |
T4950 |
2648-2649 |
-LRB- |
denotes |
( |
T4951 |
2658-2663 |
VBN |
denotes |
shown |
T4952 |
2649-2653 |
NNS |
denotes |
data |
T4953 |
2654-2657 |
RB |
denotes |
not |
T4954 |
2663-2664 |
-RRB- |
denotes |
) |
T4955 |
2664-2665 |
. |
denotes |
. |
T4956 |
2665-2908 |
sentence |
denotes |
However, the deletion removes 534 of the 602 amino acids encoded by Tbx15 (including the T-box DNA-binding domain), deH/+ animals are grossly normal, and the phenotype of deH/deH animals is identical to that described for the original allele. |
T4957 |
2666-2673 |
RB |
denotes |
However |
T4958 |
2688-2695 |
VBZ |
denotes |
removes |
T4959 |
2673-2675 |
, |
denotes |
, |
T4960 |
2675-2678 |
DT |
denotes |
the |
T4961 |
2679-2687 |
NN |
denotes |
deletion |
T4962 |
2696-2699 |
CD |
denotes |
534 |
T4963 |
2707-2710 |
CD |
denotes |
602 |
T4964 |
2700-2702 |
IN |
denotes |
of |
T4965 |
2703-2706 |
DT |
denotes |
the |
T4966 |
2717-2722 |
NNS |
denotes |
acids |
T4967 |
2711-2716 |
NN |
denotes |
amino |
T4968 |
2723-2730 |
VBN |
denotes |
encoded |
T4969 |
2731-2733 |
IN |
denotes |
by |
T4970 |
2734-2739 |
NN |
denotes |
Tbx15 |
T4971 |
2740-2741 |
-LRB- |
denotes |
( |
T4972 |
2741-2750 |
VBG |
denotes |
including |
T4973 |
2751-2754 |
DT |
denotes |
the |
T4974 |
2773-2779 |
NN |
denotes |
domain |
T4975 |
2755-2756 |
NN |
denotes |
T |
T4976 |
2757-2760 |
NN |
denotes |
box |
T4977 |
2756-2757 |
HYPH |
denotes |
- |
T4978 |
2761-2764 |
NN |
denotes |
DNA |
T4979 |
2765-2772 |
VBG |
denotes |
binding |
T4980 |
2764-2765 |
HYPH |
denotes |
- |
T4981 |
2779-2780 |
-RRB- |
denotes |
) |
T4982 |
2780-2782 |
, |
denotes |
, |
T4983 |
2782-2785 |
NN |
denotes |
deH |
T4984 |
2788-2795 |
NNS |
denotes |
animals |
T4985 |
2785-2786 |
HYPH |
denotes |
/ |
T4986 |
2786-2787 |
SYM |
denotes |
+ |
T4987 |
2796-2799 |
VBP |
denotes |
are |
T4988 |
2800-2807 |
RB |
denotes |
grossly |
T4989 |
2808-2814 |
JJ |
denotes |
normal |
T4990 |
2814-2816 |
, |
denotes |
, |
T4991 |
2816-2819 |
CC |
denotes |
and |
T4992 |
2820-2823 |
DT |
denotes |
the |
T4993 |
2824-2833 |
NN |
denotes |
phenotype |
T4994 |
2853-2855 |
VBZ |
denotes |
is |
T4995 |
2834-2836 |
IN |
denotes |
of |
T4996 |
2837-2840 |
NN |
denotes |
deH |
T4997 |
2841-2844 |
NN |
denotes |
deH |
T4998 |
2840-2841 |
HYPH |
denotes |
/ |
T4999 |
2845-2852 |
NNS |
denotes |
animals |
T5000 |
2856-2865 |
JJ |
denotes |
identical |
T5001 |
2866-2868 |
IN |
denotes |
to |
T5002 |
2869-2873 |
DT |
denotes |
that |
T5003 |
2874-2883 |
VBN |
denotes |
described |
T5004 |
2884-2887 |
IN |
denotes |
for |
T5005 |
2888-2891 |
DT |
denotes |
the |
T5006 |
2901-2907 |
NN |
denotes |
allele |
T5007 |
2892-2900 |
JJ |
denotes |
original |
T5008 |
2907-2908 |
. |
denotes |
. |
T5009 |
2908-3015 |
sentence |
denotes |
In addition, other than M6pr-ps, no other genes or transcripts have been annotated to the 216 kb deletion. |
T5010 |
2909-2911 |
IN |
denotes |
In |
T5011 |
2982-2991 |
VBN |
denotes |
annotated |
T5012 |
2912-2920 |
NN |
denotes |
addition |
T5013 |
2920-2922 |
, |
denotes |
, |
T5014 |
2922-2927 |
JJ |
denotes |
other |
T5015 |
2928-2932 |
IN |
denotes |
than |
T5016 |
2933-2937 |
NN |
denotes |
M6pr |
T5017 |
2938-2940 |
NN |
denotes |
ps |
T5018 |
2937-2938 |
HYPH |
denotes |
- |
T5019 |
2940-2942 |
, |
denotes |
, |
T5020 |
2942-2944 |
DT |
denotes |
no |
T5021 |
2951-2956 |
NNS |
denotes |
genes |
T5022 |
2945-2950 |
JJ |
denotes |
other |
T5023 |
2957-2959 |
CC |
denotes |
or |
T5024 |
2960-2971 |
NNS |
denotes |
transcripts |
T5025 |
2972-2976 |
VBP |
denotes |
have |
T5026 |
2977-2981 |
VBN |
denotes |
been |
T5027 |
2992-2994 |
IN |
denotes |
to |
T5028 |
2995-2998 |
DT |
denotes |
the |
T5029 |
3006-3014 |
NN |
denotes |
deletion |
T5030 |
2999-3002 |
CD |
denotes |
216 |
T5031 |
3003-3005 |
NN |
denotes |
kb |
T5032 |
3014-3015 |
. |
denotes |
. |
T5033 |
3015-3169 |
sentence |
denotes |
While the positional cloning work was underway, one of us (A. Russ) generated an independent mutation of Tbx15 by gene targeting in embryonic stem cells. |
T5034 |
3016-3021 |
IN |
denotes |
While |
T5035 |
3050-3053 |
VBD |
denotes |
was |
T5036 |
3022-3025 |
DT |
denotes |
the |
T5037 |
3045-3049 |
NN |
denotes |
work |
T5038 |
3026-3036 |
JJ |
denotes |
positional |
T5039 |
3037-3044 |
NN |
denotes |
cloning |
T5040 |
3084-3093 |
VBD |
denotes |
generated |
T5041 |
3054-3062 |
RB |
denotes |
underway |
T5042 |
3062-3064 |
, |
denotes |
, |
T5043 |
3064-3067 |
CD |
denotes |
one |
T5044 |
3068-3070 |
IN |
denotes |
of |
T5045 |
3071-3073 |
PRP |
denotes |
us |
T5046 |
3074-3075 |
-LRB- |
denotes |
( |
T5047 |
3075-3077 |
NNP |
denotes |
A. |
T5048 |
3078-3082 |
NNP |
denotes |
Russ |
T5049 |
3082-3083 |
-RRB- |
denotes |
) |
T5050 |
3094-3096 |
DT |
denotes |
an |
T5051 |
3109-3117 |
NN |
denotes |
mutation |
T5052 |
3097-3108 |
JJ |
denotes |
independent |
T5053 |
3118-3120 |
IN |
denotes |
of |
T5054 |
3121-3126 |
NN |
denotes |
Tbx15 |
T5055 |
3127-3129 |
IN |
denotes |
by |
T5056 |
3130-3134 |
NN |
denotes |
gene |
T5057 |
3135-3144 |
NN |
denotes |
targeting |
T5058 |
3145-3147 |
IN |
denotes |
in |
T5059 |
3148-3157 |
JJ |
denotes |
embryonic |
T5060 |
3163-3168 |
NNS |
denotes |
cells |
T5061 |
3158-3162 |
NN |
denotes |
stem |
T5062 |
3168-3169 |
. |
denotes |
. |
T5063 |
3169-3329 |
sentence |
denotes |
The targeted allele, Tbx15LacZ, carries an IRES-LacZ-neo cDNA cassette that disrupts the open reading frame at codon 154 early in the T-box domain (Figure 3D). |
T5064 |
3170-3173 |
DT |
denotes |
The |
T5065 |
3183-3189 |
NN |
denotes |
allele |
T5066 |
3174-3182 |
VBN |
denotes |
targeted |
T5067 |
3202-3209 |
VBZ |
denotes |
carries |
T5068 |
3189-3191 |
, |
denotes |
, |
T5069 |
3191-3200 |
NN |
denotes |
Tbx15LacZ |
T5070 |
3200-3202 |
, |
denotes |
, |
T5071 |
3210-3212 |
DT |
denotes |
an |
T5072 |
3232-3240 |
NN |
denotes |
cassette |
T5073 |
3213-3217 |
NN |
denotes |
IRES |
T5074 |
3218-3222 |
NN |
denotes |
LacZ |
T5075 |
3217-3218 |
HYPH |
denotes |
- |
T5076 |
3222-3223 |
HYPH |
denotes |
- |
T5077 |
3223-3226 |
JJ |
denotes |
neo |
T5078 |
3227-3231 |
NN |
denotes |
cDNA |
T5079 |
3241-3245 |
WDT |
denotes |
that |
T5080 |
3246-3254 |
VBZ |
denotes |
disrupts |
T5081 |
3255-3258 |
DT |
denotes |
the |
T5082 |
3272-3277 |
NN |
denotes |
frame |
T5083 |
3259-3263 |
JJ |
denotes |
open |
T5084 |
3264-3271 |
NN |
denotes |
reading |
T5085 |
3278-3280 |
IN |
denotes |
at |
T5086 |
3281-3286 |
NN |
denotes |
codon |
T5087 |
3287-3290 |
CD |
denotes |
154 |
T5088 |
3291-3296 |
RB |
denotes |
early |
T5089 |
3297-3299 |
IN |
denotes |
in |
T5090 |
3300-3303 |
DT |
denotes |
the |
T5091 |
3310-3316 |
NN |
denotes |
domain |
T5092 |
3304-3305 |
NN |
denotes |
T |
T5093 |
3306-3309 |
NN |
denotes |
box |
T5094 |
3305-3306 |
HYPH |
denotes |
- |
T5095 |
3317-3318 |
-LRB- |
denotes |
( |
T5096 |
3318-3324 |
NN |
denotes |
Figure |
T5097 |
3325-3327 |
CD |
denotes |
3D |
T5098 |
3327-3328 |
-RRB- |
denotes |
) |
T5099 |
3328-3329 |
. |
denotes |
. |
T5100 |
3329-3616 |
sentence |
denotes |
Animals heterozygous for the targeted allele are completely normal with regard to size, skeletal morphology, and hair-color distribution, but Tbx15LacZ/Tbx15LacZ homozygotes were noted to exhibit reduced body size and an abnormal craniofacial appearance identical to that caused by deH. |
T5101 |
3330-3337 |
NNS |
denotes |
Animals |
T5102 |
3375-3378 |
VBP |
denotes |
are |
T5103 |
3338-3350 |
JJ |
denotes |
heterozygous |
T5104 |
3351-3354 |
IN |
denotes |
for |
T5105 |
3355-3358 |
DT |
denotes |
the |
T5106 |
3368-3374 |
NN |
denotes |
allele |
T5107 |
3359-3367 |
VBN |
denotes |
targeted |
T5108 |
3379-3389 |
RB |
denotes |
completely |
T5109 |
3390-3396 |
JJ |
denotes |
normal |
T5110 |
3397-3401 |
IN |
denotes |
with |
T5111 |
3402-3408 |
NN |
denotes |
regard |
T5112 |
3409-3411 |
IN |
denotes |
to |
T5113 |
3412-3416 |
NN |
denotes |
size |
T5114 |
3416-3418 |
, |
denotes |
, |
T5115 |
3418-3426 |
JJ |
denotes |
skeletal |
T5116 |
3427-3437 |
NN |
denotes |
morphology |
T5117 |
3437-3439 |
, |
denotes |
, |
T5118 |
3439-3442 |
CC |
denotes |
and |
T5119 |
3443-3447 |
NN |
denotes |
hair |
T5120 |
3448-3453 |
NN |
denotes |
color |
T5121 |
3447-3448 |
HYPH |
denotes |
- |
T5122 |
3454-3466 |
NN |
denotes |
distribution |
T5123 |
3466-3468 |
, |
denotes |
, |
T5124 |
3468-3471 |
CC |
denotes |
but |
T5125 |
3472-3481 |
NN |
denotes |
Tbx15LacZ |
T5126 |
3482-3491 |
NN |
denotes |
Tbx15LacZ |
T5127 |
3481-3482 |
HYPH |
denotes |
/ |
T5128 |
3492-3503 |
NNS |
denotes |
homozygotes |
T5129 |
3509-3514 |
VBN |
denotes |
noted |
T5130 |
3504-3508 |
VBD |
denotes |
were |
T5131 |
3515-3517 |
TO |
denotes |
to |
T5132 |
3518-3525 |
VB |
denotes |
exhibit |
T5133 |
3526-3533 |
VBN |
denotes |
reduced |
T5134 |
3539-3543 |
NN |
denotes |
size |
T5135 |
3534-3538 |
NN |
denotes |
body |
T5136 |
3544-3547 |
CC |
denotes |
and |
T5137 |
3548-3550 |
DT |
denotes |
an |
T5138 |
3573-3583 |
NN |
denotes |
appearance |
T5139 |
3551-3559 |
JJ |
denotes |
abnormal |
T5140 |
3560-3572 |
JJ |
denotes |
craniofacial |
T5141 |
3584-3593 |
JJ |
denotes |
identical |
T5142 |
3594-3596 |
IN |
denotes |
to |
T5143 |
3597-3601 |
DT |
denotes |
that |
T5144 |
3602-3608 |
VBN |
denotes |
caused |
T5145 |
3609-3611 |
IN |
denotes |
by |
T5146 |
3612-3615 |
NN |
denotes |
deH |
T5147 |
3615-3616 |
. |
denotes |
. |
T5148 |
3616-3862 |
sentence |
denotes |
We generated Tbx15LacZ/deH compound heterozygotes; on an Aw/at background, these animals exhibited the same abnormal restriction of dorsal pigmentation at P3.5 and expanded yellow flank area as described above for deH/deH animals (see Figure 2). |
T5149 |
3617-3619 |
PRP |
denotes |
We |
T5150 |
3620-3629 |
VBD |
denotes |
generated |
T5151 |
3706-3715 |
VBD |
denotes |
exhibited |
T5152 |
3630-3639 |
NN |
denotes |
Tbx15LacZ |
T5153 |
3640-3643 |
NN |
denotes |
deH |
T5154 |
3639-3640 |
SYM |
denotes |
/ |
T5155 |
3653-3666 |
NNS |
denotes |
heterozygotes |
T5156 |
3644-3652 |
NN |
denotes |
compound |
T5157 |
3666-3667 |
: |
denotes |
; |
T5158 |
3668-3670 |
IN |
denotes |
on |
T5159 |
3671-3673 |
DT |
denotes |
an |
T5160 |
3680-3690 |
NN |
denotes |
background |
T5161 |
3674-3676 |
NN |
denotes |
Aw |
T5162 |
3677-3679 |
NN |
denotes |
at |
T5163 |
3676-3677 |
HYPH |
denotes |
/ |
T5164 |
3690-3692 |
, |
denotes |
, |
T5165 |
3692-3697 |
DT |
denotes |
these |
T5166 |
3698-3705 |
NNS |
denotes |
animals |
T5167 |
3716-3719 |
DT |
denotes |
the |
T5168 |
3734-3745 |
NN |
denotes |
restriction |
T5169 |
3720-3724 |
JJ |
denotes |
same |
T5170 |
3725-3733 |
JJ |
denotes |
abnormal |
T5171 |
3746-3748 |
IN |
denotes |
of |
T5172 |
3749-3755 |
JJ |
denotes |
dorsal |
T5173 |
3756-3768 |
NN |
denotes |
pigmentation |
T5174 |
3769-3771 |
IN |
denotes |
at |
T5175 |
3772-3776 |
NN |
denotes |
P3.5 |
T5176 |
3777-3780 |
CC |
denotes |
and |
T5177 |
3781-3789 |
VBN |
denotes |
expanded |
T5178 |
3803-3807 |
NN |
denotes |
area |
T5179 |
3790-3796 |
JJ |
denotes |
yellow |
T5180 |
3797-3802 |
NN |
denotes |
flank |
T5181 |
3808-3810 |
IN |
denotes |
as |
T5182 |
3811-3820 |
VBN |
denotes |
described |
T5183 |
3821-3826 |
RB |
denotes |
above |
T5184 |
3827-3830 |
IN |
denotes |
for |
T5185 |
3831-3834 |
NN |
denotes |
deH |
T5186 |
3835-3838 |
NN |
denotes |
deH |
T5187 |
3834-3835 |
HYPH |
denotes |
/ |
T5188 |
3839-3846 |
NNS |
denotes |
animals |
T5189 |
3847-3848 |
-LRB- |
denotes |
( |
T5190 |
3848-3851 |
VB |
denotes |
see |
T5191 |
3852-3858 |
NN |
denotes |
Figure |
T5192 |
3859-3860 |
CD |
denotes |
2 |
T5193 |
3860-3861 |
-RRB- |
denotes |
) |
T5194 |
3861-3862 |
. |
denotes |
. |
T5195 |
3862-3995 |
sentence |
denotes |
These observations demonstrate that the pigmentary and craniofacial characteristics of deH are caused by loss of function for Tbx15. |
T5196 |
3863-3868 |
DT |
denotes |
These |
T5197 |
3869-3881 |
NNS |
denotes |
observations |
T5198 |
3882-3893 |
VBP |
denotes |
demonstrate |
T5199 |
3894-3898 |
IN |
denotes |
that |
T5200 |
3958-3964 |
VBN |
denotes |
caused |
T5201 |
3899-3902 |
DT |
denotes |
the |
T5202 |
3931-3946 |
NNS |
denotes |
characteristics |
T5203 |
3903-3913 |
JJ |
denotes |
pigmentary |
T5204 |
3914-3917 |
CC |
denotes |
and |
T5205 |
3918-3930 |
JJ |
denotes |
craniofacial |
T5206 |
3947-3949 |
IN |
denotes |
of |
T5207 |
3950-3953 |
NN |
denotes |
deH |
T5208 |
3954-3957 |
VBP |
denotes |
are |
T5209 |
3965-3967 |
IN |
denotes |
by |
T5210 |
3968-3972 |
NN |
denotes |
loss |
T5211 |
3973-3975 |
IN |
denotes |
of |
T5212 |
3976-3984 |
NN |
denotes |
function |
T5213 |
3985-3988 |
IN |
denotes |
for |
T5214 |
3989-3994 |
NN |
denotes |
Tbx15 |
T5215 |
3994-3995 |
. |
denotes |
. |
R3088 |
T4613 |
T4614 |
amod |
Positional,Cloning |
R3089 |
T4615 |
T4614 |
prep |
of,Cloning |
R3090 |
T4616 |
T4615 |
pobj |
deH,of |
R3091 |
T4618 |
T4619 |
prep |
As,identified |
R3092 |
T4620 |
T4621 |
det |
a,marker |
R3093 |
T4621 |
T4618 |
pobj |
marker,As |
R3094 |
T4622 |
T4621 |
amod |
visible,marker |
R3095 |
T4623 |
T4619 |
punct |
", ",identified |
R3096 |
T4624 |
T4625 |
amod |
early,studies |
R3097 |
T4625 |
T4619 |
nsubj |
studies,identified |
R3098 |
T4626 |
T4625 |
compound |
linkage,studies |
R3099 |
T4627 |
T4625 |
prep |
with,studies |
R3100 |
T4628 |
T4629 |
det |
the,allele |
R3101 |
T4629 |
T4627 |
pobj |
allele,with |
R3102 |
T4630 |
T4629 |
amod |
original,allele |
R3103 |
T4631 |
T4632 |
amod |
droopy,ear |
R3104 |
T4632 |
T4629 |
compound |
ear,allele |
R3105 |
T4633 |
T4629 |
cc |
or,allele |
R3106 |
T4634 |
T4635 |
det |
the,allele |
R3107 |
T4635 |
T4629 |
conj |
allele,allele |
R3108 |
T4636 |
T4635 |
compound |
deH,allele |
R3109 |
T4637 |
T4638 |
det |
a,position |
R3110 |
T4638 |
T4619 |
dobj |
position,identified |
R3111 |
T4639 |
T4638 |
compound |
map,position |
R3112 |
T4640 |
T4638 |
prep |
in,position |
R3113 |
T4641 |
T4642 |
det |
the,middle |
R3114 |
T4642 |
T4640 |
pobj |
middle,in |
R3115 |
T4643 |
T4642 |
prep |
of,middle |
R3116 |
T4644 |
T4643 |
pobj |
Chromosome,of |
R3117 |
T4645 |
T4644 |
nummod |
3,Chromosome |
R3118 |
T4646 |
T4638 |
punct |
", ",position |
R3119 |
T4647 |
T4638 |
amod |
distal,position |
R3120 |
T4648 |
T4647 |
prep |
to,distal |
R3121 |
T4649 |
T4648 |
amod |
matted,to |
R3122 |
T4650 |
T4647 |
cc |
and,distal |
R3123 |
T4651 |
T4647 |
conj |
proximal,distal |
R3124 |
T4652 |
T4651 |
prep |
to,proximal |
R3125 |
T4653 |
T4654 |
compound |
Varitint,waddler |
R3126 |
T4654 |
T4652 |
pobj |
waddler,to |
R3127 |
T4655 |
T4654 |
punct |
-,waddler |
R3128 |
T4656 |
T4657 |
punct |
(,Carter |
R3129 |
T4657 |
T4619 |
meta |
Carter,identified |
R3130 |
T4658 |
T4657 |
cc |
and,Carter |
R3131 |
T4659 |
T4657 |
conj |
Falconer,Carter |
R3132 |
T4660 |
T4659 |
nummod |
1951,Falconer |
R3133 |
T4661 |
T4659 |
punct |
;,Falconer |
R3134 |
T4662 |
T4659 |
conj |
Curry,Falconer |
R3135 |
T4663 |
T4662 |
nummod |
1959,Curry |
R3136 |
T4664 |
T4662 |
punct |
;,Curry |
R3137 |
T4665 |
T4662 |
conj |
Lane,Curry |
R3138 |
T4666 |
T4665 |
cc |
and,Lane |
R3139 |
T4667 |
T4665 |
conj |
Eicher,Lane |
R3140 |
T4668 |
T4667 |
nummod |
1979,Eicher |
R3141 |
T4669 |
T4667 |
punct |
;,Eicher |
R3142 |
T4670 |
T4667 |
conj |
Holmes,Eicher |
R3143 |
T4671 |
T4670 |
nmod |
et,Holmes |
R3144 |
T4672 |
T4670 |
nmod |
al.,Holmes |
R3145 |
T4673 |
T4670 |
nummod |
1981,Holmes |
R3146 |
T4674 |
T4670 |
punct |
),Holmes |
R3147 |
T4675 |
T4619 |
punct |
.,identified |
R3148 |
T4677 |
T4678 |
nsubj |
We,used |
R3149 |
T4679 |
T4680 |
det |
an,intercross |
R3150 |
T4680 |
T4678 |
dobj |
intercross,used |
R3151 |
T4681 |
T4680 |
compound |
F2,intercross |
R3152 |
T4682 |
T4680 |
prep |
with,intercross |
R3153 |
T4683 |
T4684 |
compound |
CAST,Ei |
R3154 |
T4684 |
T4686 |
compound |
Ei,mice |
R3155 |
T4685 |
T4684 |
punct |
/,Ei |
R3156 |
T4686 |
T4682 |
pobj |
mice,with |
R3157 |
T4687 |
T4688 |
aux |
to,localize |
R3158 |
T4688 |
T4678 |
advcl |
localize,used |
R3159 |
T4689 |
T4688 |
dobj |
deH,localize |
R3160 |
T4690 |
T4688 |
prep |
to,localize |
R3161 |
T4691 |
T4692 |
det |
a,interval |
R3162 |
T4692 |
T4690 |
pobj |
interval,to |
R3163 |
T4693 |
T4694 |
nummod |
0.1,cM |
R3164 |
T4694 |
T4692 |
compound |
cM,interval |
R3165 |
T4695 |
T4692 |
prep |
between,interval |
R3166 |
T4696 |
T4695 |
pobj |
D3Mit213,between |
R3167 |
T4697 |
T4696 |
cc |
and,D3Mit213 |
R3168 |
T4698 |
T4696 |
conj |
16.MMHAP32FLF1,D3Mit213 |
R3169 |
T4699 |
T4692 |
punct |
", ",interval |
R3170 |
T4700 |
T4701 |
dep |
which,refined |
R3171 |
T4701 |
T4692 |
relcl |
refined,interval |
R3172 |
T4702 |
T4701 |
auxpass |
was,refined |
R3173 |
T4703 |
T4701 |
prep |
by,refined |
R3174 |
T4704 |
T4703 |
pobj |
development,by |
R3175 |
T4705 |
T4704 |
prep |
of,development |
R3176 |
T4706 |
T4707 |
det |
a,contig |
R3177 |
T4707 |
T4705 |
pobj |
contig,of |
R3178 |
T4708 |
T4709 |
amod |
bacterial,chromosome |
R3179 |
T4709 |
T4707 |
nmod |
chromosome,contig |
R3180 |
T4710 |
T4709 |
amod |
artificial,chromosome |
R3181 |
T4711 |
T4709 |
punct |
(,chromosome |
R3182 |
T4712 |
T4709 |
appos |
BAC,chromosome |
R3183 |
T4713 |
T4707 |
punct |
),contig |
R3184 |
T4714 |
T4707 |
cc |
and,contig |
R3185 |
T4715 |
T4716 |
amod |
additional,markers |
R3186 |
T4716 |
T4707 |
conj |
markers,contig |
R3187 |
T4717 |
T4716 |
prep |
to,markers |
R3188 |
T4718 |
T4719 |
det |
a,region |
R3189 |
T4719 |
T4717 |
pobj |
region,to |
R3190 |
T4720 |
T4721 |
nummod |
1.4,Mb |
R3191 |
T4721 |
T4719 |
compound |
Mb,region |
R3192 |
T4722 |
T4723 |
dep |
that,contained |
R3193 |
T4723 |
T4719 |
relcl |
contained,region |
R3194 |
T4724 |
T4725 |
nummod |
eight,genes |
R3195 |
T4725 |
T4723 |
dobj |
genes,contained |
R3196 |
T4726 |
T4725 |
punct |
", ",genes |
R3197 |
T4727 |
T4725 |
prep |
including,genes |
R3198 |
T4728 |
T4727 |
pobj |
Tbx15,including |
R3199 |
T4729 |
T4730 |
punct |
(,Figure |
R3200 |
T4730 |
T4688 |
parataxis |
Figure,localize |
R3201 |
T4731 |
T4730 |
nummod |
3A,Figure |
R3202 |
T4732 |
T4730 |
punct |
),Figure |
R3203 |
T4733 |
T4678 |
punct |
.,used |
R3204 |
T4735 |
T4736 |
nsubj |
We,considered |
R3205 |
T4737 |
T4736 |
dobj |
Tbx15,considered |
R3206 |
T4738 |
T4736 |
prep |
as,considered |
R3207 |
T4739 |
T4740 |
det |
an,candidate |
R3208 |
T4740 |
T4738 |
pobj |
candidate,as |
R3209 |
T4741 |
T4740 |
amod |
excellent,candidate |
R3210 |
T4742 |
T4740 |
prep |
for,candidate |
R3211 |
T4743 |
T4744 |
det |
the,abnormalities |
R3212 |
T4744 |
T4742 |
pobj |
abnormalities,for |
R3213 |
T4745 |
T4744 |
amod |
skeletal,abnormalities |
R3214 |
T4746 |
T4744 |
acl |
caused,abnormalities |
R3215 |
T4747 |
T4746 |
agent |
by,caused |
R3216 |
T4748 |
T4747 |
pobj |
deH,by |
R3217 |
T4749 |
T4736 |
punct |
", ",considered |
R3218 |
T4750 |
T4736 |
prep |
based,considered |
R3219 |
T4751 |
T4750 |
prep |
on,based |
R3220 |
T4752 |
T4751 |
pobj |
studies,on |
R3221 |
T4753 |
T4752 |
prep |
by,studies |
R3222 |
T4754 |
T4753 |
pobj |
Agulnik,by |
R3223 |
T4755 |
T4756 |
advmod |
et,al. |
R3224 |
T4756 |
T4754 |
advmod |
al.,Agulnik |
R3225 |
T4757 |
T4754 |
punct |
(,Agulnik |
R3226 |
T4758 |
T4754 |
npadvmod |
1998,Agulnik |
R3227 |
T4759 |
T4754 |
punct |
),Agulnik |
R3228 |
T4760 |
T4754 |
punct |
", ",Agulnik |
R3229 |
T4761 |
T4762 |
dep |
who,described |
R3230 |
T4762 |
T4754 |
relcl |
described,Agulnik |
R3231 |
T4763 |
T4764 |
poss |
its,expression |
R3232 |
T4764 |
T4762 |
dobj |
expression,described |
R3233 |
T4765 |
T4764 |
amod |
embryonic,expression |
R3234 |
T4766 |
T4764 |
prep |
in,expression |
R3235 |
T4767 |
T4768 |
det |
the,region |
R3236 |
T4768 |
T4766 |
pobj |
region,in |
R3237 |
T4769 |
T4768 |
amod |
craniofacial,region |
R3238 |
T4770 |
T4768 |
cc |
and,region |
R3239 |
T4771 |
T4772 |
amod |
developing,limbs |
R3240 |
T4772 |
T4768 |
conj |
limbs,region |
R3241 |
T4773 |
T4736 |
punct |
.,considered |
R3242 |
T4775 |
T4776 |
advcl |
Using,found |
R3243 |
T4777 |
T4778 |
compound |
sequence,information |
R3244 |
T4778 |
T4775 |
dobj |
information,Using |
R3245 |
T4779 |
T4778 |
prep |
from,information |
R3246 |
T4780 |
T4779 |
pobj |
Agulnik,from |
R3247 |
T4781 |
T4782 |
advmod |
et,al. |
R3248 |
T4782 |
T4780 |
advmod |
al.,Agulnik |
R3249 |
T4783 |
T4780 |
punct |
(,Agulnik |
R3250 |
T4784 |
T4780 |
npadvmod |
1998,Agulnik |
R3251 |
T4785 |
T4780 |
punct |
),Agulnik |
R3252 |
T4786 |
T4778 |
cc |
and,information |
R3253 |
T4787 |
T4788 |
det |
the,sequence |
R3255 |
T4789 |
T4790 |
advmod |
partially,completed |
R3256 |
T4790 |
T4788 |
amod |
completed,sequence |
R3257 |
T4791 |
T4792 |
compound |
mouse,genome |
R3258 |
T4792 |
T4788 |
compound |
genome,sequence |
R3259 |
T4793 |
T4776 |
punct |
", ",found |
R3260 |
T4794 |
T4776 |
nsubj |
we,found |
R3261 |
T4795 |
T4796 |
mark |
that,amplified |
R3262 |
T4796 |
T4776 |
ccomp |
amplified,found |
R3263 |
T4797 |
T4796 |
nsubjpass |
portions,amplified |
R3264 |
T4798 |
T4797 |
prep |
of,portions |
R3265 |
T4799 |
T4800 |
amod |
several,exons |
R3266 |
T4800 |
T4798 |
pobj |
exons,of |
R3267 |
T4801 |
T4800 |
compound |
Tbx15,exons |
R3268 |
T4802 |
T4796 |
aux |
could,amplified |
R3269 |
T4803 |
T4796 |
neg |
not,amplified |
R3270 |
T4804 |
T4796 |
auxpass |
be,amplified |
R3271 |
T4805 |
T4796 |
prep |
from,amplified |
R3272 |
T4806 |
T4807 |
nmod |
deH,deH |
R3273 |
T4807 |
T4809 |
nmod |
deH,DNA |
R3274 |
T4808 |
T4807 |
punct |
/,deH |
R3275 |
T4809 |
T4805 |
pobj |
DNA,from |
R3276 |
T4810 |
T4809 |
amod |
genomic,DNA |
R3277 |
T4811 |
T4776 |
punct |
.,found |
R3278 |
T4813 |
T4814 |
det |
The,gene |
R3279 |
T4814 |
T4816 |
nsubjpass |
gene,referred |
R3280 |
T4815 |
T4814 |
amod |
same,gene |
R3281 |
T4817 |
T4816 |
auxpass |
was,referred |
R3282 |
T4818 |
T4816 |
advmod |
initially,referred |
R3283 |
T4819 |
T4816 |
prep |
to,referred |
R3284 |
T4820 |
T4816 |
prep |
as,referred |
R3285 |
T4821 |
T4820 |
pobj |
Tbx8,as |
R3286 |
T4822 |
T4823 |
punct |
(,Wattler |
R3287 |
T4823 |
T4816 |
meta |
Wattler,referred |
R3288 |
T4824 |
T4823 |
nmod |
et,Wattler |
R3289 |
T4825 |
T4823 |
nmod |
al.,Wattler |
R3290 |
T4826 |
T4823 |
nummod |
1998,Wattler |
R3291 |
T4827 |
T4823 |
punct |
),Wattler |
R3292 |
T4828 |
T4816 |
cc |
and,referred |
R3293 |
T4829 |
T4830 |
advmod |
then,renamed |
R3294 |
T4830 |
T4816 |
conj |
renamed,referred |
R3295 |
T4831 |
T4830 |
advmod |
later,renamed |
R3296 |
T4832 |
T4830 |
oprd |
Tbx14,renamed |
R3297 |
T4833 |
T4830 |
punct |
", ",renamed |
R3298 |
T4834 |
T4830 |
cc |
but,renamed |
R3299 |
T4835 |
T4836 |
auxpass |
is,referred |
R3300 |
T4836 |
T4830 |
conj |
referred,renamed |
R3301 |
T4837 |
T4836 |
advmod |
currently,referred |
R3302 |
T4838 |
T4836 |
prep |
to,referred |
R3303 |
T4839 |
T4836 |
prep |
in,referred |
R3304 |
T4840 |
T4841 |
amod |
several,genomes |
R3305 |
T4841 |
T4839 |
pobj |
genomes,in |
R3306 |
T4842 |
T4841 |
compound |
vertebrate,genomes |
R3307 |
T4843 |
T4836 |
prep |
as,referred |
R3308 |
T4844 |
T4843 |
pobj |
Tbx15,as |
R3309 |
T4845 |
T4846 |
punct |
(,Agulnik |
R3310 |
T4846 |
T4836 |
meta |
Agulnik,referred |
R3311 |
T4847 |
T4846 |
nmod |
et,Agulnik |
R3312 |
T4848 |
T4846 |
nmod |
al.,Agulnik |
R3313 |
T4849 |
T4846 |
nummod |
1998,Agulnik |
R3314 |
T4850 |
T4846 |
punct |
;,Agulnik |
R3315 |
T4851 |
T4846 |
nmod |
Begemann,Agulnik |
R3316 |
T4852 |
T4846 |
nmod |
et,Agulnik |
R3317 |
T4853 |
T4846 |
nmod |
al.,Agulnik |
R3318 |
T4854 |
T4846 |
nummod |
2002,Agulnik |
R3319 |
T4855 |
T4846 |
punct |
),Agulnik |
R3320 |
T4856 |
T4816 |
punct |
.,referred |
R3321 |
T4858 |
T4859 |
prep |
By,identified |
R3322 |
T4860 |
T4858 |
pcomp |
comparing,By |
R3323 |
T4861 |
T4862 |
det |
the,sequence |
R3324 |
T4862 |
T4860 |
dobj |
sequence,comparing |
R3325 |
T4863 |
T4862 |
prep |
of,sequence |
R3326 |
T4864 |
T4865 |
det |
a,fragment |
R3327 |
T4865 |
T4863 |
pobj |
fragment,of |
R3328 |
T4866 |
T4867 |
nummod |
1.3,kb |
R3329 |
T4867 |
T4868 |
compound |
kb,junction |
R3330 |
T4868 |
T4865 |
compound |
junction,fragment |
R3331 |
T4869 |
T4865 |
acl |
amplified,fragment |
R3332 |
T4870 |
T4869 |
prep |
from,amplified |
R3333 |
T4871 |
T4872 |
nmod |
deH,deH |
R3334 |
T4872 |
T4874 |
nmod |
deH,DNA |
R3335 |
T4873 |
T4872 |
punct |
/,deH |
R3336 |
T4874 |
T4870 |
pobj |
DNA,from |
R3337 |
T4875 |
T4874 |
amod |
genomic,DNA |
R3338 |
T4876 |
T4860 |
prep |
to,comparing |
R3339 |
T4877 |
T4878 |
advmod |
publicly,available |
R3340 |
T4878 |
T4879 |
amod |
available,sequence |
R3341 |
T4879 |
T4876 |
pobj |
sequence,to |
R3342 |
T4880 |
T4881 |
compound |
mouse,genome |
R3343 |
T4881 |
T4879 |
compound |
genome,sequence |
R3344 |
T4882 |
T4859 |
punct |
", ",identified |
R3345 |
T4883 |
T4859 |
nsubj |
we,identified |
R3346 |
T4884 |
T4885 |
det |
a,deletion |
R3347 |
T4885 |
T4859 |
dobj |
deletion,identified |
R3348 |
T4886 |
T4887 |
nummod |
216,kb |
R3349 |
T4887 |
T4885 |
compound |
kb,deletion |
R3350 |
T4888 |
T4889 |
dep |
that,extends |
R3351 |
T4889 |
T4885 |
relcl |
extends,deletion |
R3352 |
T4890 |
T4889 |
prep |
from,extends |
R3353 |
T4891 |
T4892 |
compound |
Tbx15,intron |
R3354 |
T4892 |
T4890 |
pobj |
intron,from |
R3355 |
T4893 |
T4892 |
nummod |
1,intron |
R3356 |
T4894 |
T4889 |
prep |
to,extends |
R3357 |
T4895 |
T4896 |
nummod |
148,kb |
R3358 |
T4896 |
T4897 |
npadvmod |
kb,downstream |
R3359 |
T4897 |
T4894 |
pcomp |
downstream,to |
R3360 |
T4898 |
T4897 |
prep |
of,downstream |
R3361 |
T4899 |
T4900 |
det |
the,sequence |
R3362 |
T4900 |
T4898 |
pobj |
sequence,of |
R3363 |
T4901 |
T4900 |
compound |
polyadenylation,sequence |
R3364 |
T4902 |
T4900 |
prep |
in,sequence |
R3365 |
T4903 |
T4904 |
det |
a,region |
R3366 |
T4904 |
T4902 |
pobj |
region,in |
R3367 |
T4905 |
T4904 |
acl |
annotated,region |
R3368 |
T4906 |
T4905 |
prep |
as,annotated |
R3369 |
T4907 |
T4908 |
det |
a,pseudogene |
R3370 |
T4908 |
T4906 |
pobj |
pseudogene,as |
R3371 |
T4909 |
T4910 |
nmod |
mannose,phosphate |
R3372 |
T4910 |
T4914 |
compound |
phosphate,receptor |
R3373 |
T4911 |
T4910 |
punct |
-,phosphate |
R3374 |
T4912 |
T4910 |
nummod |
6,phosphate |
R3375 |
T4913 |
T4910 |
punct |
-,phosphate |
R3376 |
T4914 |
T4908 |
compound |
receptor,pseudogene |
R3377 |
T4915 |
T4908 |
punct |
", ",pseudogene |
R3378 |
T4916 |
T4917 |
compound |
M6pr,ps |
R3379 |
T4917 |
T4908 |
appos |
ps,pseudogene |
R3380 |
T4918 |
T4917 |
punct |
-,ps |
R3381 |
T4919 |
T4920 |
punct |
(,3B |
R3382 |
T4920 |
T4859 |
parataxis |
3B,identified |
R3383 |
T4921 |
T4920 |
nmod |
Figure,3B |
R3384 |
T4922 |
T4920 |
cc |
and,3B |
R3385 |
T4923 |
T4920 |
conj |
3C,3B |
R3386 |
T4924 |
T4920 |
punct |
),3B |
R3387 |
T4925 |
T4859 |
punct |
.,identified |
R3388 |
T4927 |
T4928 |
punct |
(,Ludwig |
R3389 |
T4929 |
T4928 |
nmod |
et,Ludwig |
R3390 |
T4930 |
T4928 |
nmod |
al.,Ludwig |
R3391 |
T4931 |
T4928 |
nummod |
1992,Ludwig |
R3392 |
T4932 |
T4928 |
punct |
),Ludwig |
R3393 |
T4933 |
T4928 |
punct |
.,Ludwig |
R3394 |
T4935 |
T4936 |
prep |
By,identified |
R3395 |
T4937 |
T4938 |
compound |
Northern,blot |
R3396 |
T4938 |
T4939 |
compound |
blot,analysis |
R3397 |
T4939 |
T4935 |
pobj |
analysis,By |
R3398 |
T4940 |
T4936 |
punct |
", ",identified |
R3399 |
T4941 |
T4936 |
nsubj |
we,identified |
R3400 |
T4942 |
T4943 |
det |
a,transcript |
R3401 |
T4943 |
T4936 |
dobj |
transcript,identified |
R3402 |
T4944 |
T4943 |
compound |
fusion,transcript |
R3403 |
T4945 |
T4943 |
acl |
produced,transcript |
R3404 |
T4946 |
T4945 |
prep |
from,produced |
R3405 |
T4947 |
T4948 |
det |
the,chromosome |
R3406 |
T4948 |
T4946 |
pobj |
chromosome,from |
R3407 |
T4949 |
T4948 |
compound |
deH,chromosome |
R3408 |
T4950 |
T4951 |
punct |
(,shown |
R3409 |
T4951 |
T4936 |
parataxis |
shown,identified |
R3410 |
T4952 |
T4951 |
nsubj |
data,shown |
R3411 |
T4953 |
T4951 |
neg |
not,shown |
R3412 |
T4954 |
T4951 |
punct |
),shown |
R3413 |
T4955 |
T4936 |
punct |
.,identified |
R3414 |
T4957 |
T4958 |
advmod |
However,removes |
R3415 |
T4959 |
T4958 |
punct |
", ",removes |
R3416 |
T4960 |
T4961 |
det |
the,deletion |
R3417 |
T4961 |
T4958 |
nsubj |
deletion,removes |
R3418 |
T4962 |
T4963 |
quantmod |
534,602 |
R3419 |
T4963 |
T4966 |
nummod |
602,acids |
R3420 |
T4964 |
T4963 |
quantmod |
of,602 |
R3421 |
T4965 |
T4963 |
quantmod |
the,602 |
R3422 |
T4966 |
T4958 |
dobj |
acids,removes |
R3423 |
T4967 |
T4966 |
compound |
amino,acids |
R3424 |
T4968 |
T4966 |
acl |
encoded,acids |
R3425 |
T4969 |
T4968 |
agent |
by,encoded |
R3426 |
T4970 |
T4969 |
pobj |
Tbx15,by |
R3427 |
T4971 |
T4966 |
punct |
(,acids |
R3428 |
T4972 |
T4966 |
prep |
including,acids |
R3429 |
T4973 |
T4974 |
det |
the,domain |
R3430 |
T4974 |
T4972 |
pobj |
domain,including |
R3431 |
T4975 |
T4976 |
nmod |
T,box |
R3432 |
T4976 |
T4974 |
nmod |
box,domain |
R3433 |
T4977 |
T4976 |
punct |
-,box |
R3434 |
T4978 |
T4979 |
npadvmod |
DNA,binding |
R3435 |
T4979 |
T4974 |
amod |
binding,domain |
R3436 |
T4980 |
T4979 |
punct |
-,binding |
R3437 |
T4981 |
T4958 |
punct |
),removes |
R3438 |
T4982 |
T4958 |
punct |
", ",removes |
R3439 |
T4983 |
T4984 |
nmod |
deH,animals |
R3440 |
T4984 |
T4987 |
nsubj |
animals,are |
R3441 |
T4985 |
T4983 |
punct |
/,deH |
R3442 |
T4986 |
T4983 |
punct |
+,deH |
R3443 |
T4987 |
T4958 |
conj |
are,removes |
R3444 |
T4988 |
T4989 |
advmod |
grossly,normal |
R3445 |
T4989 |
T4987 |
acomp |
normal,are |
R3446 |
T4990 |
T4987 |
punct |
", ",are |
R3447 |
T4991 |
T4987 |
cc |
and,are |
R3448 |
T4992 |
T4993 |
det |
the,phenotype |
R3449 |
T4993 |
T4994 |
nsubj |
phenotype,is |
R3450 |
T4994 |
T4987 |
conj |
is,are |
R3451 |
T4995 |
T4993 |
prep |
of,phenotype |
R3452 |
T4996 |
T4997 |
compound |
deH,deH |
R3453 |
T4997 |
T4999 |
compound |
deH,animals |
R3454 |
T4998 |
T4997 |
punct |
/,deH |
R3455 |
T4999 |
T4995 |
pobj |
animals,of |
R3456 |
T5000 |
T4994 |
acomp |
identical,is |
R3457 |
T5001 |
T5000 |
prep |
to,identical |
R3458 |
T5002 |
T5001 |
pobj |
that,to |
R3459 |
T5003 |
T5002 |
acl |
described,that |
R3460 |
T5004 |
T5003 |
prep |
for,described |
R3461 |
T5005 |
T5006 |
det |
the,allele |
R3462 |
T5006 |
T5004 |
pobj |
allele,for |
R3463 |
T5007 |
T5006 |
amod |
original,allele |
R3464 |
T5008 |
T4994 |
punct |
.,is |
R3465 |
T5010 |
T5011 |
prep |
In,annotated |
R3466 |
T5012 |
T5010 |
pobj |
addition,In |
R3467 |
T5013 |
T5011 |
punct |
", ",annotated |
R3468 |
T5014 |
T5011 |
amod |
other,annotated |
R3469 |
T5015 |
T5014 |
prep |
than,other |
R3470 |
T5016 |
T5017 |
compound |
M6pr,ps |
R3471 |
T5017 |
T5015 |
pobj |
ps,than |
R3472 |
T5018 |
T5017 |
punct |
-,ps |
R3473 |
T5019 |
T5011 |
punct |
", ",annotated |
R3474 |
T5020 |
T5021 |
det |
no,genes |
R3475 |
T5021 |
T5011 |
nsubjpass |
genes,annotated |
R3476 |
T5022 |
T5021 |
amod |
other,genes |
R3477 |
T5023 |
T5021 |
cc |
or,genes |
R3478 |
T5024 |
T5021 |
conj |
transcripts,genes |
R3479 |
T5025 |
T5011 |
aux |
have,annotated |
R3480 |
T5026 |
T5011 |
auxpass |
been,annotated |
R3481 |
T5027 |
T5011 |
prep |
to,annotated |
R3482 |
T5028 |
T5029 |
det |
the,deletion |
R3483 |
T5029 |
T5027 |
pobj |
deletion,to |
R3484 |
T5030 |
T5031 |
nummod |
216,kb |
R3485 |
T5031 |
T5029 |
compound |
kb,deletion |
R3486 |
T5032 |
T5011 |
punct |
.,annotated |
R3487 |
T5034 |
T5035 |
mark |
While,was |
R3489 |
T5036 |
T5037 |
det |
the,work |
R3490 |
T5037 |
T5035 |
nsubj |
work,was |
R3491 |
T5038 |
T5037 |
amod |
positional,work |
R3492 |
T5039 |
T5037 |
compound |
cloning,work |
R3493 |
T5041 |
T5035 |
advmod |
underway,was |
R3494 |
T5042 |
T5040 |
punct |
", ",generated |
R3495 |
T5043 |
T5040 |
nsubj |
one,generated |
R3496 |
T5044 |
T5043 |
prep |
of,one |
R3497 |
T5045 |
T5044 |
pobj |
us,of |
R3498 |
T5046 |
T5043 |
punct |
(,one |
R3499 |
T5047 |
T5048 |
compound |
A.,Russ |
R3500 |
T5048 |
T5043 |
appos |
Russ,one |
R3501 |
T5049 |
T5043 |
punct |
),one |
R3502 |
T5050 |
T5051 |
det |
an,mutation |
R3503 |
T5051 |
T5040 |
dobj |
mutation,generated |
R3504 |
T5052 |
T5051 |
amod |
independent,mutation |
R3505 |
T5053 |
T5051 |
prep |
of,mutation |
R3506 |
T5054 |
T5053 |
pobj |
Tbx15,of |
R3507 |
T5055 |
T5040 |
prep |
by,generated |
R3508 |
T5056 |
T5057 |
compound |
gene,targeting |
R3509 |
T5057 |
T5055 |
pobj |
targeting,by |
R3510 |
T5058 |
T5057 |
prep |
in,targeting |
R3511 |
T5059 |
T5060 |
amod |
embryonic,cells |
R3512 |
T5060 |
T5058 |
pobj |
cells,in |
R3513 |
T5061 |
T5060 |
compound |
stem,cells |
R3514 |
T5062 |
T5040 |
punct |
.,generated |
R3515 |
T5064 |
T5065 |
det |
The,allele |
R3516 |
T5065 |
T5067 |
nsubj |
allele,carries |
R3517 |
T5066 |
T5065 |
amod |
targeted,allele |
R3518 |
T5068 |
T5065 |
punct |
", ",allele |
R3519 |
T5069 |
T5065 |
appos |
Tbx15LacZ,allele |
R3520 |
T5070 |
T5067 |
punct |
", ",carries |
R3521 |
T5071 |
T5072 |
det |
an,cassette |
R3522 |
T5072 |
T5067 |
dobj |
cassette,carries |
R3523 |
T5073 |
T5074 |
nmod |
IRES,LacZ |
R3524 |
T5074 |
T5072 |
nmod |
LacZ,cassette |
R3525 |
T5075 |
T5074 |
punct |
-,LacZ |
R3526 |
T5076 |
T5074 |
punct |
-,LacZ |
R3527 |
T5077 |
T5074 |
amod |
neo,LacZ |
R3528 |
T5078 |
T5072 |
compound |
cDNA,cassette |
R3529 |
T5079 |
T5080 |
dep |
that,disrupts |
R3530 |
T5080 |
T5072 |
relcl |
disrupts,cassette |
R3531 |
T5081 |
T5082 |
det |
the,frame |
R3532 |
T5082 |
T5080 |
dobj |
frame,disrupts |
R3533 |
T5083 |
T5082 |
amod |
open,frame |
R3534 |
T5084 |
T5082 |
compound |
reading,frame |
R3535 |
T5085 |
T5082 |
prep |
at,frame |
R3536 |
T5086 |
T5085 |
pobj |
codon,at |
R3537 |
T5087 |
T5086 |
nummod |
154,codon |
R3538 |
T5088 |
T5080 |
advmod |
early,disrupts |
R3539 |
T5089 |
T5080 |
prep |
in,disrupts |
R3540 |
T5090 |
T5091 |
det |
the,domain |
R3541 |
T5091 |
T5089 |
pobj |
domain,in |
R3542 |
T5092 |
T5093 |
compound |
T,box |
R3543 |
T5093 |
T5091 |
compound |
box,domain |
R3544 |
T5094 |
T5093 |
punct |
-,box |
R3545 |
T5095 |
T5096 |
punct |
(,Figure |
R3546 |
T5096 |
T5080 |
parataxis |
Figure,disrupts |
R3547 |
T5097 |
T5096 |
nummod |
3D,Figure |
R3548 |
T5098 |
T5096 |
punct |
),Figure |
R3549 |
T5099 |
T5067 |
punct |
.,carries |
R3550 |
T5101 |
T5102 |
nsubj |
Animals,are |
R3551 |
T5103 |
T5101 |
amod |
heterozygous,Animals |
R3552 |
T5104 |
T5103 |
prep |
for,heterozygous |
R3553 |
T5105 |
T5106 |
det |
the,allele |
R3554 |
T5106 |
T5104 |
pobj |
allele,for |
R3555 |
T5107 |
T5106 |
amod |
targeted,allele |
R3556 |
T5108 |
T5109 |
advmod |
completely,normal |
R3557 |
T5109 |
T5102 |
acomp |
normal,are |
R3558 |
T5110 |
T5102 |
prep |
with,are |
R3559 |
T5111 |
T5110 |
pobj |
regard,with |
R3560 |
T5112 |
T5111 |
prep |
to,regard |
R3561 |
T5113 |
T5112 |
pobj |
size,to |
R3562 |
T5114 |
T5113 |
punct |
", ",size |
R3563 |
T5115 |
T5116 |
amod |
skeletal,morphology |
R3564 |
T5116 |
T5113 |
conj |
morphology,size |
R3565 |
T5117 |
T5116 |
punct |
", ",morphology |
R3566 |
T5118 |
T5116 |
cc |
and,morphology |
R3567 |
T5119 |
T5120 |
compound |
hair,color |
R3568 |
T5120 |
T5122 |
compound |
color,distribution |
R3569 |
T5121 |
T5120 |
punct |
-,color |
R3570 |
T5122 |
T5116 |
conj |
distribution,morphology |
R3571 |
T5123 |
T5102 |
punct |
", ",are |
R3572 |
T5124 |
T5102 |
cc |
but,are |
R3573 |
T5125 |
T5126 |
compound |
Tbx15LacZ,Tbx15LacZ |
R3574 |
T5126 |
T5128 |
compound |
Tbx15LacZ,homozygotes |
R3575 |
T5127 |
T5126 |
punct |
/,Tbx15LacZ |
R3576 |
T5128 |
T5129 |
nsubjpass |
homozygotes,noted |
R3577 |
T5129 |
T5102 |
conj |
noted,are |
R3578 |
T5130 |
T5129 |
auxpass |
were,noted |
R3579 |
T5131 |
T5132 |
aux |
to,exhibit |
R3580 |
T5132 |
T5129 |
xcomp |
exhibit,noted |
R3581 |
T5133 |
T5134 |
amod |
reduced,size |
R3582 |
T5134 |
T5132 |
dobj |
size,exhibit |
R3583 |
T5135 |
T5134 |
compound |
body,size |
R3584 |
T5136 |
T5134 |
cc |
and,size |
R3585 |
T5137 |
T5138 |
det |
an,appearance |
R3586 |
T5138 |
T5134 |
conj |
appearance,size |
R3587 |
T5139 |
T5138 |
amod |
abnormal,appearance |
R3588 |
T5140 |
T5138 |
amod |
craniofacial,appearance |
R3589 |
T5141 |
T5138 |
amod |
identical,appearance |
R3590 |
T5142 |
T5141 |
prep |
to,identical |
R3591 |
T5143 |
T5142 |
pobj |
that,to |
R3592 |
T5144 |
T5143 |
acl |
caused,that |
R3593 |
T5145 |
T5144 |
agent |
by,caused |
R3594 |
T5146 |
T5145 |
pobj |
deH,by |
R3595 |
T5147 |
T5129 |
punct |
.,noted |
R3596 |
T5149 |
T5150 |
nsubj |
We,generated |
R3597 |
T5150 |
T5151 |
ccomp |
generated,exhibited |
R3598 |
T5152 |
T5153 |
nmod |
Tbx15LacZ,deH |
R3599 |
T5153 |
T5155 |
compound |
deH,heterozygotes |
R3600 |
T5154 |
T5153 |
punct |
/,deH |
R3601 |
T5155 |
T5150 |
dobj |
heterozygotes,generated |
R3602 |
T5156 |
T5155 |
compound |
compound,heterozygotes |
R3603 |
T5157 |
T5151 |
punct |
;,exhibited |
R3604 |
T5158 |
T5151 |
prep |
on,exhibited |
R3605 |
T5159 |
T5160 |
det |
an,background |
R3606 |
T5160 |
T5158 |
pobj |
background,on |
R3607 |
T5161 |
T5162 |
compound |
Aw,at |
R3608 |
T5162 |
T5160 |
compound |
at,background |
R3609 |
T5163 |
T5162 |
punct |
/,at |
R3610 |
T5164 |
T5151 |
punct |
", ",exhibited |
R3611 |
T5165 |
T5166 |
det |
these,animals |
R3612 |
T5166 |
T5151 |
nsubj |
animals,exhibited |
R3613 |
T5167 |
T5168 |
det |
the,restriction |
R3614 |
T5168 |
T5151 |
dobj |
restriction,exhibited |
R3615 |
T5169 |
T5168 |
amod |
same,restriction |
R3616 |
T5170 |
T5168 |
amod |
abnormal,restriction |
R3617 |
T5171 |
T5168 |
prep |
of,restriction |
R3618 |
T5172 |
T5173 |
amod |
dorsal,pigmentation |
R3619 |
T5173 |
T5171 |
pobj |
pigmentation,of |
R3620 |
T5174 |
T5168 |
prep |
at,restriction |
R3621 |
T5175 |
T5174 |
pobj |
P3.5,at |
R3622 |
T5176 |
T5168 |
cc |
and,restriction |
R3623 |
T5177 |
T5178 |
amod |
expanded,area |
R3624 |
T5178 |
T5168 |
conj |
area,restriction |
R3625 |
T5179 |
T5178 |
amod |
yellow,area |
R3626 |
T5180 |
T5178 |
compound |
flank,area |
R3627 |
T5181 |
T5182 |
mark |
as,described |
R3628 |
T5182 |
T5151 |
advcl |
described,exhibited |
R3629 |
T5183 |
T5182 |
advmod |
above,described |
R3630 |
T5184 |
T5182 |
prep |
for,described |
R3631 |
T5185 |
T5186 |
compound |
deH,deH |
R3632 |
T5186 |
T5188 |
compound |
deH,animals |
R3633 |
T5187 |
T5186 |
punct |
/,deH |
R3634 |
T5188 |
T5184 |
pobj |
animals,for |
R3635 |
T5189 |
T5190 |
punct |
(,see |
R3636 |
T5190 |
T5151 |
parataxis |
see,exhibited |
R3637 |
T5191 |
T5190 |
dobj |
Figure,see |
R3638 |
T5192 |
T5191 |
nummod |
2,Figure |
R3639 |
T5193 |
T5190 |
punct |
),see |
R3640 |
T5194 |
T5151 |
punct |
.,exhibited |
R3641 |
T5196 |
T5197 |
det |
These,observations |
R3642 |
T5197 |
T5198 |
nsubj |
observations,demonstrate |
R3643 |
T5199 |
T5200 |
mark |
that,caused |
R3644 |
T5200 |
T5198 |
ccomp |
caused,demonstrate |
R3645 |
T5201 |
T5202 |
det |
the,characteristics |
R3646 |
T5202 |
T5200 |
nsubjpass |
characteristics,caused |
R3647 |
T5203 |
T5202 |
amod |
pigmentary,characteristics |
R3648 |
T5204 |
T5203 |
cc |
and,pigmentary |
R3649 |
T5205 |
T5203 |
conj |
craniofacial,pigmentary |
R3650 |
T5206 |
T5202 |
prep |
of,characteristics |
R3651 |
T5207 |
T5206 |
pobj |
deH,of |
R3652 |
T5208 |
T5200 |
auxpass |
are,caused |
R3653 |
T5209 |
T5200 |
agent |
by,caused |
R3654 |
T5210 |
T5209 |
pobj |
loss,by |
R3655 |
T5211 |
T5210 |
prep |
of,loss |
R3656 |
T5212 |
T5211 |
pobj |
function,of |
R3657 |
T5213 |
T5210 |
prep |
for,loss |
R3658 |
T5214 |
T5213 |
pobj |
Tbx15,for |
R3659 |
T5215 |
T5198 |
punct |
.,demonstrate |
R3254 |
T4788 |
T4778 |
conj |
sequence,information |
R3488 |
T5035 |
T5040 |
advcl |
was,generated |