PMC:3091641 / 7121-7667 JSONTXT

{"project":"2_test","denotations":[{"id":"20663124-9799794-10298385","span":{"begin":175,"end":177},"obj":"9799794"}],"text":"The consequence of incomplete coverage is the exacerbation of one of the shortcomings of whole-genome shotgun sequencing: the difficulty of resolving repetitive DNA segments [28]. If two sequencing traces contain regions of similarity, it may be difficult or impossible to determine whether these traces are derived from the same underlying DNA or from two distinct DNA segments that are themselves paralogous. This difficulty is not limited to the assembly of highly repetitious intergenic regions, but to the inference of gene families as well."}