PMC:3091639 / 34072-35021 JSONTXT

{"project":"2_test","denotations":[{"id":"20646324-7984417-10032222","span":{"begin":243,"end":245},"obj":"7984417"},{"id":"20646324-17846036-10032223","span":{"begin":539,"end":541},"obj":"17846036"}],"text":"Analysis of nucleotide and amino acid sequences and phylogeny\nGene alignments were performed using the Clustal W program (MacVector 10.6 Genetics Computer Group, Madison, Wisconcin) to identify polymorphisms and indels (insertions/deletions) [62]. Conserved residues are outlined, similar amino acids are shaded in grey, mismatches are not shaded and dashes correspond to gaps in the sequence. A consensus line appears at the bottom of the alignment. Pmp sequences were subjected to multiple sequence alignment using ClustalW2 (EMBL-EBI) [63] and BioEdit Sequence Alignment Editor [64]. SNP position analysis for each group was performed using the Microsoft Excel program (Microsoft Corporation). Motif Scan [65] was used to identify motifs in a sequence.\nA phylogenetic tree was constructed by Neighbor-Joining, tie breaking = systematic, distance corrected by the Poisson method with gaps distributed proportionally and 1,000 bootstrap replicates."}