PMC:3091636 / 15419-16941 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/3091636","sourcedb":"PMC","sourceid":"3091636","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3091636","text":"Sequences for CXCR1-5 have previously been published. The sequences we detected mostly had synonymous differences compared to those published except for CXCR5 (GU936966) which had three amino acid changes compared to the published sequence (NP_001011675); one in the extracellular amino-terminus, one in the second extracellular loop and one in the intracellular carboxy-terminus. There are multiple sequences available for CXCR6 in the NCBI database (AAX08649, NP_001014859, AAX08913), and one in the ENSEMBL database (ENSBTAG00000015708) which is identical at the amino acid level to NP_001014859 although synonymous nucleotide differences are present between the two sequences. All three sequences deposited in the NCBI database differ at the amino acid level and our sequence (GU936967) is also different from these. The sequences deposited in GenBank are all from pooled samples which may account for the sequence differences seen. The sequence for XCR1 is currently only predicted from the database (XM_870210). We amplified an XCR1 transcript (GU936968) which has one amino acid change, compared to the predicted sequence, in the second intracellular loop. Experimentally confirmed sequence has been published for CX3CR1 (AAI48891) but this is from a Hereford animal. Our sequence (GU936969) differed from the published sequence by three amino acids, located in the extracellular amino-terminus, the second transmembrane domain and the fourth transmembrane domain. These are likely to be breed-specific differences.","tracks":[]}