PMC:3091627 / 17924-19428 JSONTXT

{"project":"2_test","denotations":[{"id":"20537153-15516563-10781742","span":{"begin":294,"end":296},"obj":"15516563"},{"id":"20537153-16789826-10781743","span":{"begin":842,"end":844},"obj":"16789826"}],"text":"Conversely, loss of synteny was also due to the presence of 110 CDSs in B38 that were not present in Shi470. Forty-three of these CDSs appeared as clusters within eight loci. Twenty corresponded to ISHp609 (5 complete and conserved copies of ISHp609 each comprising orf1, orf2, ORFA and ORFB) [31], which interrupts HELPY0571, HELPY0700 (both encoding restriction/modification systems), HELPY0838 (encoding a putative Rad50 ATPase), HELPY1330 (encoding a putative glycosyl-transferase), and HELPY1529 (a HAC prophage II protein homolog). In addition to these five ISHp609 insertions, loss of synteny was also due to the presence of CDSs in four other loci: i) a cluster of seven genes (HELPY1520 to HELPY1525 and HEPLY1527, HELPY1528 to HELPY1533) encoding HacII prophage-like proteins similar to those found in H. acinonychis strain Sheeba [34]; however, the size of the prophage is much larger (32 CDS) in this species, suggesting that the prophage in B38 has been deleted, possibly following the insertion of one copy of ISHp609; ii) a cluster of six genes encoding hypothetical proteins of unknown function (HELPY0051 to HELPY0056); iii) a cluster of three CDSs that are absent in Shi470, HPAG1, J99, P12, and G127, but present in strain 26695, of which two encode alginate-O-acetylation proteins (HELPY0497-498); iv) a cluster of seven CDSs that encode a putative helicase (HELPY0989) and a putative serine kinase (HELPY0990), two functional proteins not found in all of the other sequenced strains."}