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PMC:3062687 / 33741-35129 JSONTXT

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bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T22800 132-134 P0A7Z4 denotes pH

2_test

Id Subject Object Predicate Lexical cue
21399639-18045535-74765193 954-955 18045535 denotes 6
21399639-18045535-74765193 954-955 18045535 denotes 6

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T22550 0-19 NN denotes Immunoprecipitation
T22551 20-23 CC denotes and
T22552 24-34 NN denotes immunoblot
T22553 35-38 DT denotes The
T22554 39-44 NN denotes cells
T22555 45-49 VB denotes were
T22556 50-59 VB denotes harvested
T22557 60-63 CC denotes and
T22558 64-69 VB denotes lysed
T22559 70-72 IN denotes on
T22560 73-76 NN denotes ice
T22561 77-79 IN denotes by
T22562 80-83 CD denotes 0.4
T22563 84-86 NN denotes ml
T22564 87-89 IN denotes of
T22565 90-93 DT denotes the
T22566 94-102 VB denotes modified
T22567 103-107 NN denotes RIPA
T22568 108-114 NN denotes buffer
T22569 115-116 -LRB- denotes (
T22570 116-118 CD denotes 50
T22571 119-121 NN denotes mM
T22572 122-130 NN denotes Tris-HCl
T22573 131-132 -LRB- denotes [
T22574 132-134 NN denotes pH
T22575 135-138 CD denotes 7.4
T22576 138-139 -RRB- denotes ]
T22577 139-140 -COMMA- denotes ,
T22578 141-142 CD denotes 1
T22579 142-143 NN denotes %
T22580 144-149 NN denotes NP-40
T22581 149-150 -COMMA- denotes ,
T22582 151-155 CD denotes 0.25
T22583 155-156 NN denotes %
T22584 157-172 JJ denotes Na-deoxycholate
T22585 172-173 -COMMA- denotes ,
T22586 174-177 CD denotes 150
T22587 178-180 NN denotes mM
T22588 181-185 NN denotes NaCl
T22589 185-186 -COMMA- denotes ,
T22590 187-188 CD denotes 1
T22591 189-191 NN denotes mM
T22592 192-196 NN denotes EDTA
T22593 196-197 -COMMA- denotes ,
T22594 198-199 CD denotes 1
T22595 200-202 NN denotes mM
T22596 203-207 NN denotes PMSF
T22597 207-208 -COMMA- denotes ,
T22598 209-210 CD denotes 1
T22599 211-213 NN denotes mM
T22600 214-220 NN denotes Na3VO4
T22601 220-221 -COMMA- denotes ,
T22602 222-223 CD denotes 1
T22603 224-226 NN denotes mM
T22604 227-230 NN denotes NaF
T22605 230-231 -RRB- denotes )
T22606 232-244 VB denotes supplemented
T22607 245-249 IN denotes with
T22608 250-251 CD denotes 1
T22609 252-253 SYM denotes ×
T22610 254-262 NN denotes protease
T22611 263-272 NN denotes inhibitor
T22612 273-281 NN denotes cocktail
T22613 282-283 -LRB- denotes (
T22614 283-288 NN denotes Roche
T22615 288-289 -RRB- denotes )
T22616 290-293 CC denotes and
T22617 294-295 CD denotes 1
T22618 296-297 CC denotes ×
T22619 298-309 NN denotes phosphatase
T22620 310-319 NN denotes inhibitor
T22621 320-328 NN denotes cocktail
T22622 329-332 NN denotes set
T22623 333-334 CD denotes I
T22624 335-336 -LRB- denotes (
T22625 336-339 NNP denotes EMD
T22626 340-351 NNP denotes Biosciences
T22627 351-352 -RRB- denotes )
T22628 353-356 IN denotes for
T22629 357-359 CD denotes 30
T22630 360-363 NN denotes min
T22631 365-368 DT denotes The
T22632 369-376 NN denotes lysates
T22633 377-381 VB denotes were
T22634 382-393 VB denotes centrifuged
T22635 394-396 IN denotes at
T22636 397-403 CD denotes 10,000
T22637 404-405 SYM denotes ×
T22638 406-407 NN denotes g
T22639 408-410 IN denotes at
T22640 411-412 CD denotes 4
T22641 413-415 NN denotes °C
T22642 416-419 IN denotes for
T22643 420-422 CD denotes 10
T22644 423-426 NN denotes min
T22645 427-429 TO denotes to
T22646 430-436 VB denotes remove
T22647 437-446 JJ denotes insoluble
T22648 447-455 NN denotes material
T22649 457-462 IN denotes After
T22650 463-474 VB denotes normalizing
T22651 475-482 NN denotes protein
T22652 483-497 NN denotes concentrations
T22653 497-498 -COMMA- denotes ,
T22654 499-506 NN denotes lysates
T22655 507-511 VB denotes were
T22656 512-521 VB denotes subjected
T22657 522-524 TO denotes to
T22658 525-544 NN denotes immunoprecipitation
T22659 545-547 IN denotes by
T22660 548-554 VB denotes adding
T22661 555-557 CD denotes 10
T22662 558-563 NN denotes mg/ml
T22663 564-575 JJ denotes appropriate
T22664 576-584 NN denotes antibody
T22665 585-589 CC denotes plus
T22666 590-592 CD denotes 30
T22667 593-595 NN denotes ml
T22668 596-598 IN denotes of
T22669 599-606 NN denotes protein
T22670 607-616 NN denotes G-agarose
T22671 617-618 -LRB- denotes (
T22672 618-623 NNP denotes Roche
T22673 623-624 -RRB- denotes )
T22674 624-625 -COMMA- denotes ,
T22675 626-629 CC denotes and
T22676 630-637 VB denotes rotated
T22677 638-641 IN denotes for
T22678 642-644 IN denotes at
T22679 645-650 JJ denotes least
T22680 651-652 CD denotes 2
T22681 653-654 NN denotes h
T22682 655-657 IN denotes at
T22683 658-662 NNP denotes 4°C.
T22684 663-666 DT denotes The
T22685 667-679 NN denotes precipitates
T22686 680-684 VB denotes were
T22687 685-691 VB denotes washed
T22688 692-694 IN denotes at
T22689 695-700 JJ denotes least
T22690 701-705 CD denotes five
T22691 706-711 NN denotes times
T22692 712-716 IN denotes with
T22693 717-721 JJ denotes cold
T22694 722-727 NN denotes lysis
T22695 728-734 NN denotes buffer
T22696 735-743 VB denotes followed
T22697 744-746 IN denotes by
T22698 747-757 NN denotes separation
T22699 758-760 IN denotes by
T22700 761-769 NN denotes SDS-PAGE
T22701 770-775 IN denotes under
T22702 776-783 VB denotes reduced
T22703 784-787 CC denotes and
T22704 788-798 VB denotes denaturing
T22705 799-809 NN denotes conditions
T22706 811-825 NN denotes Nitrocellulose
T22707 826-835 NN denotes membranes
T22708 836-840 VB denotes were
T22709 841-848 VB denotes blocked
T22710 849-851 IN denotes in
T22711 852-853 CD denotes 5
T22712 854-855 NN denotes %
T22713 856-862 JJ denotes nonfat
T22714 863-867 NN denotes milk
T22715 868-870 IN denotes in
T22716 871-874 CD denotes 0.1
T22717 875-876 NN denotes %
T22718 877-886 JJ denotes PBS-Tween
T22719 887-889 CD denotes 20
T22720 890-891 -LRB- denotes (
T22721 891-896 NN denotes PBS-T
T22722 896-897 -RRB- denotes )
T22723 897-898 -COMMA- denotes ,
T22724 899-905 VB denotes probed
T22725 906-910 IN denotes with
T22726 911-919 JJ denotes specific
T22727 920-930 NN denotes antibodies
T22728 931-933 IN denotes as
T22729 934-943 VB denotes described
T22730 944-955 NN denotes previously6
T22731 957-960 IN denotes For
T22732 961-975 NN denotes immunoblotting
T22733 976-978 IN denotes of
T22734 979-993 VB denotes phosphorylated
T22735 994-1002 NN denotes proteins
T22736 1002-1003 -COMMA- denotes ,
T22737 1004-1008 NN denotes gels
T22738 1009-1013 VB denotes were
T22739 1014-1025 VB denotes transferred
T22740 1026-1028 TO denotes to
T22741 1029-1045 JJ denotes methanol-treated
T22742 1046-1060 NN denotes polyvinylidene
T22743 1061-1069 NN denotes chloride
T22744 1070-1079 NN denotes membranes
T22745 1079-1080 -COMMA- denotes ,
T22746 1081-1090 VB denotes retreated
T22747 1091-1095 IN denotes with
T22748 1096-1104 NN denotes methanol
T22749 1104-1105 -COMMA- denotes ,
T22750 1106-1109 CC denotes and
T22751 1110-1115 VB denotes dried
T22752 1116-1119 IN denotes for
T22753 1120-1122 CD denotes 30
T22754 1123-1126 NN denotes min
T22755 1128-1133 NN denotes Blots
T22756 1134-1138 VB denotes were
T22757 1139-1146 VB denotes blocked
T22758 1147-1149 IN denotes in
T22759 1150-1151 CD denotes 5
T22760 1152-1153 NN denotes %
T22761 1154-1160 JJ denotes bovine
T22762 1161-1166 NN denotes serum
T22763 1167-1174 NN denotes albumin
T22764 1175-1177 IN denotes in
T22765 1178-1181 CD denotes 0.1
T22766 1182-1183 NN denotes %
T22767 1184-1188 NNP denotes Tris
T22768 1189-1197 VB denotes buffered
T22769 1198-1210 JJ denotes saline-Tween
T22770 1211-1213 CD denotes 20
T22771 1214-1215 -LRB- denotes (
T22772 1215-1220 NN denotes TBS-T
T22773 1220-1221 -RRB- denotes )
T22774 1221-1222 -COMMA- denotes ,
T22775 1223-1226 CC denotes and
T22776 1227-1233 VB denotes probed
T22777 1234-1238 IN denotes with
T22778 1239-1247 JJ denotes specific
T22779 1248-1258 NN denotes antibodies
T22780 1259-1261 IN denotes as
T22781 1262-1271 VB denotes described
T22782 1272-1284 NN denotes previously46
T22783 1286-1291 NN denotes Bands
T22784 1292-1296 VB denotes were
T22785 1297-1303 VB denotes imaged
T22786 1304-1306 IN denotes by
T22787 1307-1310 DT denotes the
T22788 1311-1316 NNP denotes Super
T22789 1317-1326 NN denotes Signaling
T22790 1327-1333 NN denotes system
T22791 1334-1335 -LRB- denotes (
T22792 1335-1341 NN denotes Pierce
T22793 1341-1342 -RRB- denotes )
T22794 1343-1352 VB denotes according
T22795 1353-1355 TO denotes to
T22796 1356-1359 DT denotes the
T22797 1360-1372 NN denotes manufacturer
T22798 1372-1374 POS denotes 's
T22799 1375-1387 NN denotes instructions
R17971 T22550 T22551 arg1Of Immunoprecipitation,and
R17972 T22552 T22551 arg2Of immunoblot,and
R17973 T22554 T22553 arg1Of cells,The
R17974 T22554 T22555 arg1Of cells,were
R17975 T22554 T22556 arg2Of cells,harvested
R17976 T22554 T22558 arg2Of cells,lysed
R17977 T22556 T22557 arg1Of harvested,and
R17978 T22557 T22555 arg2Of and,were
R17979 T22558 T22557 arg2Of lysed,and
R17980 T22558 T22559 arg1Of lysed,on
R17981 T22558 T22561 arg1Of lysed,by
R17982 T22560 T22559 arg2Of ice,on
R17983 T22563 T22561 arg2Of ml,by
R17984 T22563 T22562 arg1Of ml,0.4
R17985 T22563 T22564 arg1Of ml,of
R17986 T22568 T22564 arg2Of buffer,of
R17987 T22568 T22565 arg1Of buffer,the
R17988 T22568 T22566 arg2Of buffer,modified
R17989 T22568 T22567 arg1Of buffer,RIPA
R17990 T22568 T22569 arg1Of buffer,(
R17991 T22568 T22606 arg2Of buffer,supplemented
R17992 T22570 T22571 arg1Of 50,mM
R17993 T22572 T22570 arg1Of Tris-HCl,50
R17994 T22572 T22573 arg1Of Tris-HCl,[
R17995 T22572 T22577 arg1Of Tris-HCl,","
R17996 T22574 T22573 arg2Of pH,[
R17997 T22574 T22575 arg1Of pH,7.4
R17998 T22576 T22573 arg3Of ],[
R17999 T22577 T22569 arg2Of ",",(
R18000 T22577 T22581 arg1Of ",",","
R18001 T22578 T22579 arg1Of 1,%
R18002 T22580 T22577 arg2Of NP-40,","
R18003 T22580 T22578 arg1Of NP-40,1
R18004 T22582 T22583 arg1Of 0.25,%
R18005 T22582 T22584 arg1Of 0.25,Na-deoxycholate
R18006 T22582 T22585 arg1Of 0.25,","
R18007 T22586 T22585 arg2Of 150,","
R18008 T22586 T22587 arg1Of 150,mM
R18009 T22588 T22582 arg1Of NaCl,0.25
R18010 T22588 T22586 arg1Of NaCl,150
R18011 T22588 T22589 arg1Of NaCl,","
R18012 T22589 T22593 arg1Of ",",","
R18013 T22590 T22591 arg1Of 1,mM
R18014 T22592 T22589 arg2Of EDTA,","
R18015 T22592 T22590 arg1Of EDTA,1
R18016 T22593 T22597 arg1Of ",",","
R18017 T22594 T22595 arg1Of 1,mM
R18018 T22596 T22593 arg2Of PMSF,","
R18019 T22596 T22594 arg1Of PMSF,1
R18020 T22597 T22601 arg1Of ",",","
R18021 T22598 T22599 arg1Of 1,mM
R18022 T22600 T22597 arg2Of Na3VO4,","
R18023 T22600 T22598 arg1Of Na3VO4,1
R18024 T22601 T22581 arg2Of ",",","
R18025 T22602 T22603 arg1Of 1,mM
R18026 T22604 T22601 arg2Of NaF,","
R18027 T22604 T22602 arg1Of NaF,1
R18028 T22605 T22569 arg3Of ),(
R18029 T22606 T22607 arg1Of supplemented,with
R18030 T22606 T22628 arg1Of supplemented,for
R18031 T22612 T22608 arg1Of cocktail,1
R18032 T22612 T22609 arg1Of cocktail,×
R18033 T22612 T22610 arg1Of cocktail,protease
R18034 T22612 T22611 arg1Of cocktail,inhibitor
R18035 T22612 T22613 arg1Of cocktail,(
R18036 T22612 T22616 arg1Of cocktail,and
R18037 T22614 T22613 arg2Of Roche,(
R18038 T22615 T22613 arg3Of ),(
R18039 T22616 T22607 arg2Of and,with
R18040 T22617 T22618 arg1Of 1,×
R18041 T22619 T22618 arg2Of phosphatase,×
R18042 T22622 T22616 arg2Of set,and
R18043 T22622 T22617 arg1Of set,1
R18044 T22622 T22619 arg1Of set,phosphatase
R18045 T22622 T22620 arg1Of set,inhibitor
R18046 T22622 T22621 arg1Of set,cocktail
R18047 T22622 T22623 arg1Of set,I
R18048 T22622 T22624 arg1Of set,(
R18049 T22626 T22624 arg2Of Biosciences,(
R18050 T22626 T22625 arg1Of Biosciences,EMD
R18051 T22627 T22624 arg3Of ),(
R18052 T22630 T22628 arg2Of min,for
R18053 T22630 T22629 arg1Of min,30
R18054 T22632 T22631 arg1Of lysates,The
R18055 T22632 T22633 arg1Of lysates,were
R18056 T22632 T22634 arg2Of lysates,centrifuged
R18057 T22634 T22633 arg2Of centrifuged,were
R18058 T22634 T22635 arg1Of centrifuged,at
R18059 T22638 T22635 arg2Of g,at
R18060 T22638 T22636 arg1Of g,"10,000"
R18061 T22638 T22637 arg1Of g,×
R18062 T22638 T22639 arg1Of g,at
R18063 T22638 T22642 arg1Of g,for
R18064 T22641 T22639 arg2Of °C,at
R18065 T22641 T22640 arg1Of °C,4
R18066 T22644 T22642 arg2Of min,for
R18067 T22644 T22643 arg1Of min,10
R18068 T22644 T22646 arg1Of min,remove
R18069 T22646 T22642 arg3Of remove,for
R18070 T22646 T22645 arg1Of remove,to
R18071 T22648 T22646 arg2Of material,remove
R18072 T22648 T22647 arg1Of material,insoluble
R18073 T22650 T22649 arg2Of normalizing,After
R18074 T22652 T22650 arg2Of concentrations,normalizing
R18075 T22652 T22651 arg1Of concentrations,protein
R18076 T22654 T22650 arg1Of lysates,normalizing
R18077 T22654 T22655 arg1Of lysates,were
R18078 T22654 T22656 arg2Of lysates,subjected
R18079 T22654 T22660 arg1Of lysates,adding
R18080 T22654 T22676 arg2Of lysates,rotated
R18081 T22656 T22657 arg1Of subjected,to
R18082 T22656 T22659 arg1Of subjected,by
R18083 T22656 T22675 arg1Of subjected,and
R18084 T22658 T22657 arg2Of immunoprecipitation,to
R18085 T22660 T22659 arg2Of adding,by
R18086 T22660 T22671 arg1Of adding,(
R18087 T22664 T22661 arg1Of antibody,10
R18088 T22664 T22662 arg1Of antibody,mg/ml
R18089 T22664 T22663 arg1Of antibody,appropriate
R18090 T22664 T22665 arg1Of antibody,plus
R18091 T22665 T22660 arg2Of plus,adding
R18092 T22667 T22665 arg2Of ml,plus
R18093 T22667 T22666 arg1Of ml,30
R18094 T22667 T22668 arg1Of ml,of
R18095 T22670 T22668 arg2Of G-agarose,of
R18096 T22670 T22669 arg1Of G-agarose,protein
R18097 T22672 T22671 arg2Of Roche,(
R18098 T22673 T22671 arg3Of ),(
R18099 T22675 T22649 arg1Of and,After
R18100 T22675 T22653 arg1Of and,","
R18101 T22675 T22655 arg2Of and,were
R18102 T22675 T22674 arg1Of and,","
R18103 T22676 T22675 arg2Of rotated,and
R18104 T22676 T22677 arg1Of rotated,for
R18105 T22676 T22691 arg1Of rotated,times
R18106 T22680 T22678 arg1Of 2,at
R18107 T22680 T22679 arg1Of 2,least
R18108 T22681 T22677 arg2Of h,for
R18109 T22681 T22680 arg1Of h,2
R18110 T22681 T22682 arg1Of h,at
R18111 T22683 T22682 arg2Of 4°C.,at
R18112 T22683 T22686 modOf 4°C.,were
R18113 T22685 T22684 arg1Of precipitates,The
R18114 T22685 T22686 arg1Of precipitates,were
R18115 T22685 T22687 arg2Of precipitates,washed
R18116 T22687 T22686 arg2Of washed,were
R18117 T22690 T22688 arg1Of five,at
R18118 T22690 T22689 arg1Of five,least
R18119 T22691 T22690 arg1Of times,five
R18120 T22691 T22692 arg1Of times,with
R18121 T22695 T22692 arg2Of buffer,with
R18122 T22695 T22693 arg1Of buffer,cold
R18123 T22695 T22694 arg1Of buffer,lysis
R18124 T22695 T22696 arg2Of buffer,followed
R18125 T22696 T22697 arg1Of followed,by
R18126 T22696 T22701 arg1Of followed,under
R18127 T22698 T22697 arg2Of separation,by
R18128 T22700 T22696 arg1Of SDS-PAGE,followed
R18129 T22700 T22699 arg2Of SDS-PAGE,by
R18130 T22705 T22701 arg2Of conditions,under
R18131 T22705 T22702 arg2Of conditions,reduced
R18132 T22705 T22703 arg1Of conditions,and
R18133 T22705 T22704 arg1Of conditions,denaturing
R18134 T22707 T22706 arg1Of membranes,Nitrocellulose
R18135 T22707 T22708 arg1Of membranes,were
R18136 T22707 T22709 arg2Of membranes,blocked
R18137 T22709 T22708 arg2Of blocked,were
R18138 T22709 T22710 arg1Of blocked,in
R18139 T22711 T22712 arg1Of 5,%
R18140 T22714 T22710 arg2Of milk,in
R18141 T22714 T22711 arg1Of milk,5
R18142 T22714 T22713 arg1Of milk,nonfat
R18143 T22714 T22715 arg1Of milk,in
R18144 T22716 T22717 arg1Of 0.1,%
R18145 T22719 T22715 arg2Of 20,in
R18146 T22719 T22716 arg1Of 20,0.1
R18147 T22719 T22718 arg1Of 20,PBS-Tween
R18148 T22719 T22720 arg1Of 20,(
R18149 T22719 T22723 arg1Of 20,","
R18150 T22719 T22724 arg2Of 20,probed
R18151 T22721 T22720 arg2Of PBS-T,(
R18152 T22722 T22720 arg3Of ),(
R18153 T22724 T22725 arg1Of probed,with
R18154 T22727 T22725 arg2Of antibodies,with
R18155 T22727 T22726 arg1Of antibodies,specific
R18156 T22727 T22728 arg1Of antibodies,as
R18157 T22730 T22728 arg2Of previously6,as
R18158 T22730 T22729 arg2Of previously6,described
R18159 T22732 T22731 arg2Of immunoblotting,For
R18160 T22732 T22733 arg1Of immunoblotting,of
R18161 T22735 T22733 arg2Of proteins,of
R18162 T22735 T22734 arg2Of proteins,phosphorylated
R18163 T22737 T22738 arg1Of gels,were
R18164 T22737 T22739 arg2Of gels,transferred
R18165 T22737 T22751 arg1Of gels,dried
R18166 T22739 T22738 arg2Of transferred,were
R18167 T22739 T22740 arg1Of transferred,to
R18168 T22739 T22750 arg1Of transferred,and
R18169 T22744 T22740 arg2Of membranes,to
R18170 T22744 T22741 arg1Of membranes,methanol-treated
R18171 T22744 T22742 arg1Of membranes,polyvinylidene
R18172 T22744 T22743 arg1Of membranes,chloride
R18173 T22744 T22745 arg1Of membranes,","
R18174 T22744 T22746 arg2Of membranes,retreated
R18175 T22746 T22747 arg1Of retreated,with
R18176 T22748 T22747 arg2Of methanol,with
R18177 T22750 T22731 arg1Of and,For
R18178 T22750 T22736 arg1Of and,","
R18179 T22750 T22749 arg1Of and,","
R18180 T22751 T22750 arg2Of dried,and
R18181 T22751 T22752 arg1Of dried,for
R18182 T22754 T22752 arg2Of min,for
R18183 T22754 T22753 arg1Of min,30
R18184 T22755 T22756 arg1Of Blots,were
R18185 T22755 T22757 arg2Of Blots,blocked
R18186 T22755 T22776 arg2Of Blots,probed
R18187 T22757 T22758 arg1Of blocked,in
R18188 T22757 T22764 arg1Of blocked,in
R18189 T22757 T22770 arg1Of blocked,20
R18190 T22757 T22775 arg1Of blocked,and
R18191 T22759 T22760 arg1Of 5,%
R18192 T22763 T22758 arg2Of albumin,in
R18193 T22763 T22759 arg1Of albumin,5
R18194 T22763 T22761 arg1Of albumin,bovine
R18195 T22763 T22762 arg1Of albumin,serum
R18196 T22766 T22764 arg2Of %,in
R18197 T22766 T22765 arg1Of %,0.1
R18198 T22766 T22768 arg2Of %,buffered
R18199 T22767 T22768 arg1Of Tris,buffered
R18200 T22770 T22769 arg1Of 20,saline-Tween
R18201 T22770 T22771 arg1Of 20,(
R18202 T22772 T22771 arg2Of TBS-T,(
R18203 T22773 T22771 arg3Of ),(
R18204 T22775 T22756 arg2Of and,were
R18205 T22775 T22774 arg1Of and,","
R18206 T22776 T22775 arg2Of probed,and
R18207 T22776 T22777 arg1Of probed,with
R18208 T22776 T22780 arg1Of probed,as
R18209 T22779 T22777 arg2Of antibodies,with
R18210 T22779 T22778 arg1Of antibodies,specific
R18211 T22782 T22780 arg2Of previously46,as
R18212 T22782 T22781 arg2Of previously46,described
R18213 T22783 T22784 arg1Of Bands,were
R18214 T22783 T22785 arg2Of Bands,imaged
R18215 T22785 T22784 arg2Of imaged,were
R18216 T22785 T22794 arg1Of imaged,according
R18217 T22790 T22785 arg1Of system,imaged
R18218 T22790 T22786 arg2Of system,by
R18219 T22790 T22787 arg1Of system,the
R18220 T22790 T22788 arg1Of system,Super
R18221 T22790 T22789 arg1Of system,Signaling
R18222 T22790 T22791 arg1Of system,(
R18223 T22792 T22791 arg2Of Pierce,(
R18224 T22793 T22791 arg3Of ),(
R18225 T22795 T22794 arg2Of to,according
R18226 T22797 T22796 arg1Of manufacturer,the
R18227 T22797 T22798 arg2Of manufacturer,'s
R18228 T22799 T22795 arg2Of instructions,to
R18229 T22799 T22798 arg1Of instructions,'s

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T22801 0-19 NNP denotes Immunoprecipitation
T22802 20-23 CC denotes and
T22803 24-34 JJ denotes immunoblot
T22804 35-38 DT denotes The
T22805 39-44 NNS denotes cells
T22806 45-49 VBD denotes were
T22807 50-59 VBN denotes harvested
T22808 60-63 CC denotes and
T22809 64-69 VBN denotes lysed
T22810 70-72 IN denotes on
T22811 73-76 NN denotes ice
T22812 77-79 IN denotes by
T22813 80-83 CD denotes 0.4
T22814 84-86 NN denotes ml
T22815 87-89 IN denotes of
T22816 90-93 DT denotes the
T22817 94-102 VBN denotes modified
T22818 103-107 NNP denotes RIPA
T22819 108-114 NN denotes buffer
T22820 115-116 -LRB- denotes (
T22821 116-118 CD denotes 50
T22822 119-121 NNP denotes mM
T22823 122-130 NNP denotes Tris-HCl
T22824 131-132 NNP denotes [
T22825 132-134 NNP denotes pH
T22826 135-138 CD denotes 7.4
T22827 138-139 NNP denotes ]
T22828 139-140 , denotes ,
T22829 141-142 CD denotes 1
T22830 142-143 NN denotes %
T22831 144-149 NN denotes NP-40
T22832 149-150 , denotes ,
T22833 151-155 CD denotes 0.25
T22834 155-156 NN denotes %
T22835 157-172 JJ denotes Na-deoxycholate
T22836 172-173 , denotes ,
T22837 174-177 CD denotes 150
T22838 178-180 NNP denotes mM
T22839 181-185 NNP denotes NaCl
T22840 185-186 , denotes ,
T22841 187-188 CD denotes 1
T22842 189-191 NNP denotes mM
T22843 192-196 NNP denotes EDTA
T22844 196-197 , denotes ,
T22845 198-199 CD denotes 1
T22846 200-202 NNP denotes mM
T22847 203-207 NNP denotes PMSF
T22848 207-208 , denotes ,
T22849 209-210 CD denotes 1
T22850 211-213 NNP denotes mM
T22851 214-220 NNP denotes Na3VO4
T22852 220-221 , denotes ,
T22853 222-223 CD denotes 1
T22854 224-226 NNP denotes mM
T22855 227-230 NNP denotes NaF
T22856 230-231 -RRB- denotes )
T22857 232-244 VBN denotes supplemented
T22858 245-249 IN denotes with
T22859 250-251 CD denotes 1
T22860 252-253 CD denotes ×
T22861 254-262 NN denotes protease
T22862 263-272 NN denotes inhibitor
T22863 273-281 NN denotes cocktail
T22864 282-283 -LRB- denotes (
T22865 283-288 NNP denotes Roche
T22866 288-289 -RRB- denotes )
T22867 290-293 CC denotes and
T22868 294-295 CD denotes 1
T22869 296-297 CD denotes ×
T22870 298-309 NN denotes phosphatase
T22871 310-319 NN denotes inhibitor
T22872 320-328 NN denotes cocktail
T22873 329-332 VBN denotes set
T22874 333-334 PRP denotes I
T22875 335-336 -LRB- denotes (
T22876 336-339 NNP denotes EMD
T22877 340-351 NNPS denotes Biosciences
T22878 351-352 -RRB- denotes )
T22879 353-356 IN denotes for
T22880 357-359 CD denotes 30
T22881 360-363 NN denotes min
T22882 363-364 . denotes .
T22883 365-368 DT denotes The
T22884 369-376 NNS denotes lysates
T22885 377-381 VBD denotes were
T22886 382-393 VBN denotes centrifuged
T22887 394-396 IN denotes at
T22888 397-403 CD denotes 10,000
T22889 404-405 CD denotes ×
T22890 406-407 NN denotes g
T22891 408-410 IN denotes at
T22892 411-412 CD denotes 4
T22893 413-414 CD denotes °
T22894 414-415 NNP denotes C
T22895 416-419 IN denotes for
T22896 420-422 CD denotes 10
T22897 423-426 NN denotes min
T22898 427-429 TO denotes to
T22899 430-436 VB denotes remove
T22900 437-446 JJ denotes insoluble
T22901 447-455 NN denotes material
T22902 455-456 . denotes .
T22903 457-462 IN denotes After
T22904 463-474 VBG denotes normalizing
T22905 475-482 NN denotes protein
T22906 483-497 NNS denotes concentrations
T22907 497-498 , denotes ,
T22908 499-506 NNS denotes lysates
T22909 507-511 VBD denotes were
T22910 512-521 VBN denotes subjected
T22911 522-524 TO denotes to
T22912 525-544 NN denotes immunoprecipitation
T22913 545-547 IN denotes by
T22914 548-554 VBG denotes adding
T22915 555-557 CD denotes 10
T22916 558-563 JJ denotes mg/ml
T22917 564-575 JJ denotes appropriate
T22918 576-584 NN denotes antibody
T22919 585-589 CC denotes plus
T22920 590-592 CD denotes 30
T22921 593-595 NN denotes ml
T22922 596-598 IN denotes of
T22923 599-606 NN denotes protein
T22924 607-616 NN denotes G-agarose
T22925 617-618 -LRB- denotes (
T22926 618-623 NNP denotes Roche
T22927 623-624 -RRB- denotes )
T22928 624-625 , denotes ,
T22929 626-629 CC denotes and
T22930 630-637 VBD denotes rotated
T22931 638-641 IN denotes for
T22932 642-644 IN denotes at
T22933 645-650 JJS denotes least
T22934 651-652 CD denotes 2
T22935 653-654 NN denotes h
T22936 655-657 IN denotes at
T22937 658-659 CD denotes 4
T22938 659-660 CD denotes °
T22939 660-661 NNP denotes C
T22940 661-662 . denotes .
T22941 663-666 DT denotes The
T22942 667-679 NNS denotes precipitates
T22943 680-684 VBD denotes were
T22944 685-691 VBN denotes washed
T22945 692-694 IN denotes at
T22946 695-700 JJS denotes least
T22947 701-705 CD denotes five
T22948 706-711 NNS denotes times
T22949 712-716 IN denotes with
T22950 717-721 JJ denotes cold
T22951 722-727 NN denotes lysis
T22952 728-734 NN denotes buffer
T22953 735-743 VBN denotes followed
T22954 744-746 IN denotes by
T22955 747-757 NN denotes separation
T22956 758-760 IN denotes by
T22957 761-769 NNP denotes SDS-PAGE
T22958 770-775 IN denotes under
T22959 776-783 VBN denotes reduced
T22960 784-787 CC denotes and
T22961 788-798 VBG denotes denaturing
T22962 799-809 NNS denotes conditions
T22963 809-810 . denotes .
T22964 811-825 NNP denotes Nitrocellulose
T22965 826-835 NNS denotes membranes
T22966 836-840 VBD denotes were
T22967 841-848 VBN denotes blocked
T22968 849-851 IN denotes in
T22969 852-853 CD denotes 5
T22970 854-855 NN denotes %
T22971 856-862 JJ denotes nonfat
T22972 863-867 NN denotes milk
T22973 868-870 IN denotes in
T22974 871-874 CD denotes 0.1
T22975 875-876 NN denotes %
T22976 877-886 JJ denotes PBS-Tween
T22977 887-889 CD denotes 20
T22978 890-891 -LRB- denotes (
T22979 891-896 NN denotes PBS-T
T22980 896-897 -RRB- denotes )
T22981 897-898 , denotes ,
T22982 899-905 VBN denotes probed
T22983 906-910 IN denotes with
T22984 911-919 JJ denotes specific
T22985 920-930 NNS denotes antibodies
T22986 931-933 IN denotes as
T22987 934-943 VBN denotes described
T22988 944-955 NNS denotes previously6
T22989 955-956 . denotes .
T22990 957-960 IN denotes For
T22991 961-975 VBG denotes immunoblotting
T22992 976-978 IN denotes of
T22993 979-993 JJ denotes phosphorylated
T22994 994-1002 NNS denotes proteins
T22995 1002-1003 , denotes ,
T22996 1004-1008 NNS denotes gels
T22997 1009-1013 VBD denotes were
T22998 1014-1025 VBN denotes transferred
T22999 1026-1028 TO denotes to
T23000 1029-1045 JJ denotes methanol-treated
T23001 1046-1060 NN denotes polyvinylidene
T23002 1061-1069 NN denotes chloride
T23003 1070-1079 NNS denotes membranes
T23004 1079-1080 , denotes ,
T23005 1081-1090 VBD denotes retreated
T23006 1091-1095 IN denotes with
T23007 1096-1104 NN denotes methanol
T23008 1104-1105 , denotes ,
T23009 1106-1109 CC denotes and
T23010 1110-1115 VBD denotes dried
T23011 1116-1119 IN denotes for
T23012 1120-1122 CD denotes 30
T23013 1123-1126 NN denotes min
T23014 1126-1127 . denotes .
T23015 1128-1133 NNS denotes Blots
T23016 1134-1138 VBD denotes were
T23017 1139-1146 VBN denotes blocked
T23018 1147-1149 IN denotes in
T23019 1150-1151 CD denotes 5
T23020 1152-1153 NN denotes %
T23021 1154-1160 JJ denotes bovine
T23022 1161-1166 NN denotes serum
T23023 1167-1174 NN denotes albumin
T23024 1175-1177 IN denotes in
T23025 1178-1181 CD denotes 0.1
T23026 1182-1183 NN denotes %
T23027 1184-1188 NNP denotes Tris
T23028 1189-1197 VBD denotes buffered
T23029 1198-1210 JJ denotes saline-Tween
T23030 1211-1213 CD denotes 20
T23031 1214-1215 -LRB- denotes (
T23032 1215-1220 NN denotes TBS-T
T23033 1220-1221 -RRB- denotes )
T23034 1221-1222 , denotes ,
T23035 1223-1226 CC denotes and
T23036 1227-1233 VBD denotes probed
T23037 1234-1238 IN denotes with
T23038 1239-1247 JJ denotes specific
T23039 1248-1258 NNS denotes antibodies
T23040 1259-1261 IN denotes as
T23041 1262-1271 VBN denotes described
T23042 1272-1284 NNS denotes previously46
T23043 1284-1285 . denotes .
T23044 1286-1291 NNS denotes Bands
T23045 1292-1296 VBD denotes were
T23046 1297-1303 VBN denotes imaged
T23047 1304-1306 IN denotes by
T23048 1307-1310 DT denotes the
T23049 1311-1316 NNP denotes Super
T23050 1317-1326 VBG denotes Signaling
T23051 1327-1333 NN denotes system
T23052 1334-1335 -LRB- denotes (
T23053 1335-1341 NNP denotes Pierce
T23054 1341-1342 -RRB- denotes )
T23055 1343-1352 VBG denotes according
T23056 1353-1355 TO denotes to
T23057 1356-1359 DT denotes the
T23058 1360-1372 NN denotes manufacturer
T23059 1372-1374 POS denotes 's
T23060 1375-1387 NNS denotes instructions
T23061 1387-1388 . denotes .
R18230 T22801 T22807 nsubjpass Immunoprecipitation,harvested
R18231 T22802 T22801 cc and,Immunoprecipitation
R18232 T22803 T22801 conj immunoblot,Immunoprecipitation
R18233 T22804 T22805 det The,cells
R18234 T22805 T22807 nsubjpass cells,harvested
R18235 T22806 T22807 auxpass were,harvested
R18236 T22807 T22807 ROOT harvested,harvested
R18237 T22808 T22807 cc and,harvested
R18238 T22809 T22807 conj lysed,harvested
R18239 T22810 T22809 prep on,lysed
R18240 T22811 T22810 pobj ice,on
R18241 T22812 T22809 prep by,lysed
R18242 T22813 T22814 nummod 0.4,ml
R18243 T22814 T22812 pobj ml,by
R18244 T22815 T22814 prep of,ml
R18245 T22816 T22819 det the,buffer
R18246 T22817 T22819 amod modified,buffer
R18247 T22818 T22819 compound RIPA,buffer
R18248 T22819 T22815 pobj buffer,of
R18249 T22820 T22827 punct (,]
R18250 T22821 T22827 nummod 50,]
R18251 T22822 T22825 compound mM,pH
R18252 T22823 T22825 compound Tris-HCl,pH
R18253 T22824 T22825 compound [,pH
R18254 T22825 T22827 nmod pH,]
R18255 T22826 T22827 nummod 7.4,]
R18256 T22827 T22819 appos ],buffer
R18257 T22828 T22827 punct ",",]
R18258 T22829 T22830 nummod 1,%
R18259 T22830 T22831 compound %,NP-40
R18260 T22831 T22827 appos NP-40,]
R18261 T22832 T22831 punct ",",NP-40
R18262 T22833 T22834 nummod 0.25,%
R18263 T22834 T22835 nsubj %,Na-deoxycholate
R18264 T22835 T22831 conj Na-deoxycholate,NP-40
R18265 T22836 T22835 punct ",",Na-deoxycholate
R18266 T22837 T22839 nummod 150,NaCl
R18267 T22838 T22839 compound mM,NaCl
R18268 T22839 T22835 appos NaCl,Na-deoxycholate
R18269 T22840 T22839 punct ",",NaCl
R18270 T22841 T22843 nummod 1,EDTA
R18271 T22842 T22843 compound mM,EDTA
R18272 T22843 T22839 appos EDTA,NaCl
R18273 T22844 T22843 punct ",",EDTA
R18274 T22845 T22847 nummod 1,PMSF
R18275 T22846 T22847 compound mM,PMSF
R18276 T22847 T22843 appos PMSF,EDTA
R18277 T22848 T22847 punct ",",PMSF
R18278 T22849 T22851 nummod 1,Na3VO4
R18279 T22850 T22851 compound mM,Na3VO4
R18280 T22851 T22847 appos Na3VO4,PMSF
R18281 T22852 T22851 punct ",",Na3VO4
R18282 T22853 T22855 nummod 1,NaF
R18283 T22854 T22855 compound mM,NaF
R18284 T22855 T22851 appos NaF,Na3VO4
R18285 T22856 T22857 punct ),supplemented
R18286 T22857 T22807 conj supplemented,harvested
R18287 T22858 T22857 prep with,supplemented
R18288 T22859 T22863 nummod 1,cocktail
R18289 T22860 T22863 nummod ×,cocktail
R18290 T22861 T22863 compound protease,cocktail
R18291 T22862 T22863 compound inhibitor,cocktail
R18292 T22863 T22858 pobj cocktail,with
R18293 T22864 T22863 punct (,cocktail
R18294 T22865 T22863 appos Roche,cocktail
R18295 T22866 T22863 punct ),cocktail
R18296 T22867 T22863 cc and,cocktail
R18297 T22868 T22869 nummod 1,×
R18298 T22869 T22872 nummod ×,cocktail
R18299 T22870 T22872 nmod phosphatase,cocktail
R18300 T22871 T22872 compound inhibitor,cocktail
R18301 T22872 T22863 conj cocktail,cocktail
R18302 T22873 T22857 conj set,supplemented
R18303 T22874 T22873 dobj I,set
R18304 T22875 T22877 punct (,Biosciences
R18305 T22876 T22877 compound EMD,Biosciences
R18306 T22877 T22874 appos Biosciences,I
R18307 T22878 T22873 punct ),set
R18308 T22879 T22873 prep for,set
R18309 T22880 T22881 nummod 30,min
R18310 T22881 T22879 pobj min,for
R18311 T22882 T22807 punct .,harvested
R18312 T22883 T22884 det The,lysates
R18313 T22884 T22886 nsubjpass lysates,centrifuged
R18314 T22885 T22886 auxpass were,centrifuged
R18315 T22886 T22886 ROOT centrifuged,centrifuged
R18316 T22887 T22886 prep at,centrifuged
R18317 T22888 T22889 nummod "10,000",×
R18318 T22889 T22887 pobj ×,at
R18319 T22890 T22886 advmod g,centrifuged
R18320 T22891 T22886 prep at,centrifuged
R18321 T22892 T22893 nummod 4,°
R18322 T22893 T22891 pobj °,at
R18323 T22894 T22886 conj C,centrifuged
R18324 T22895 T22899 mark for,remove
R18325 T22896 T22897 nummod 10,min
R18326 T22897 T22899 nsubj min,remove
R18327 T22898 T22899 aux to,remove
R18328 T22899 T22886 advcl remove,centrifuged
R18329 T22900 T22901 amod insoluble,material
R18330 T22901 T22899 dobj material,remove
R18331 T22902 T22886 punct .,centrifuged
R18332 T22903 T22910 prep After,subjected
R18333 T22904 T22903 pcomp normalizing,After
R18334 T22905 T22906 compound protein,concentrations
R18335 T22906 T22904 dobj concentrations,normalizing
R18336 T22907 T22910 punct ",",subjected
R18337 T22908 T22910 nsubjpass lysates,subjected
R18338 T22909 T22910 auxpass were,subjected
R18339 T22910 T22910 ROOT subjected,subjected
R18340 T22911 T22910 prep to,subjected
R18341 T22912 T22911 pobj immunoprecipitation,to
R18342 T22913 T22910 prep by,subjected
R18343 T22914 T22913 pcomp adding,by
R18344 T22915 T22918 nummod 10,antibody
R18345 T22916 T22918 amod mg/ml,antibody
R18346 T22917 T22918 amod appropriate,antibody
R18347 T22918 T22914 dobj antibody,adding
R18348 T22919 T22918 cc plus,antibody
R18349 T22920 T22921 nummod 30,ml
R18350 T22921 T22918 conj ml,antibody
R18351 T22922 T22921 prep of,ml
R18352 T22923 T22922 pobj protein,of
R18353 T22924 T22921 conj G-agarose,ml
R18354 T22925 T22926 punct (,Roche
R18355 T22926 T22924 appos Roche,G-agarose
R18356 T22927 T22924 punct ),G-agarose
R18357 T22928 T22910 punct ",",subjected
R18358 T22929 T22910 cc and,subjected
R18359 T22930 T22910 conj rotated,subjected
R18360 T22931 T22930 prep for,rotated
R18361 T22932 T22933 advmod at,least
R18362 T22933 T22934 advmod least,2
R18363 T22934 T22935 nummod 2,h
R18364 T22935 T22931 pobj h,for
R18365 T22936 T22930 prep at,rotated
R18366 T22937 T22938 nummod 4,°
R18367 T22938 T22936 pobj °,at
R18368 T22939 T22930 conj C,rotated
R18369 T22940 T22910 punct .,subjected
R18370 T22941 T22942 det The,precipitates
R18371 T22942 T22944 nsubjpass precipitates,washed
R18372 T22943 T22944 auxpass were,washed
R18373 T22944 T22944 ROOT washed,washed
R18374 T22945 T22946 advmod at,least
R18375 T22946 T22947 advmod least,five
R18376 T22947 T22949 nummod five,with
R18377 T22948 T22947 quantmod times,five
R18378 T22949 T22944 prep with,washed
R18379 T22950 T22952 amod cold,buffer
R18380 T22951 T22952 compound lysis,buffer
R18381 T22952 T22949 pobj buffer,with
R18382 T22953 T22952 acl followed,buffer
R18383 T22954 T22953 agent by,followed
R18384 T22955 T22954 pobj separation,by
R18385 T22956 T22955 prep by,separation
R18386 T22957 T22956 pobj SDS-PAGE,by
R18387 T22958 T22953 prep under,followed
R18388 T22959 T22962 amod reduced,conditions
R18389 T22960 T22959 cc and,reduced
R18390 T22961 T22962 compound denaturing,conditions
R18391 T22962 T22958 pobj conditions,under
R18392 T22963 T22944 punct .,washed
R18393 T22964 T22965 compound Nitrocellulose,membranes
R18394 T22965 T22967 nsubjpass membranes,blocked
R18395 T22966 T22967 auxpass were,blocked
R18396 T22967 T22967 ROOT blocked,blocked
R18397 T22968 T22967 prep in,blocked
R18398 T22969 T22970 nummod 5,%
R18399 T22970 T22972 compound %,milk
R18400 T22971 T22972 compound nonfat,milk
R18401 T22972 T22968 pobj milk,in
R18402 T22973 T22967 prep in,blocked
R18403 T22974 T22975 nummod 0.1,%
R18404 T22975 T22982 npadvmod %,probed
R18405 T22976 T22977 quantmod PBS-Tween,20
R18406 T22977 T22982 nsubj 20,probed
R18407 T22978 T22977 punct (,20
R18408 T22979 T22977 quantmod PBS-T,20
R18409 T22980 T22977 punct ),20
R18410 T22981 T22977 punct ",",20
R18411 T22982 T22967 conj probed,blocked
R18412 T22983 T22982 prep with,probed
R18413 T22984 T22985 amod specific,antibodies
R18414 T22985 T22983 pobj antibodies,with
R18415 T22986 T22987 mark as,described
R18416 T22987 T22982 advcl described,probed
R18417 T22988 T22987 dobj previously6,described
R18418 T22989 T22982 punct .,probed
R18419 T22990 T22998 prep For,transferred
R18420 T22991 T22990 pcomp immunoblotting,For
R18421 T22992 T22991 prep of,immunoblotting
R18422 T22993 T22994 amod phosphorylated,proteins
R18423 T22994 T22992 pobj proteins,of
R18424 T22995 T22998 punct ",",transferred
R18425 T22996 T22998 nsubjpass gels,transferred
R18426 T22997 T22998 auxpass were,transferred
R18427 T22998 T22998 ROOT transferred,transferred
R18428 T22999 T22998 prep to,transferred
R18429 T23000 T23003 amod methanol-treated,membranes
R18430 T23001 T23003 compound polyvinylidene,membranes
R18431 T23002 T23003 compound chloride,membranes
R18432 T23003 T22999 pobj membranes,to
R18433 T23004 T22998 punct ",",transferred
R18434 T23005 T22998 conj retreated,transferred
R18435 T23006 T23005 prep with,retreated
R18436 T23007 T23006 pobj methanol,with
R18437 T23008 T23005 punct ",",retreated
R18438 T23009 T23005 cc and,retreated
R18439 T23010 T23005 conj dried,retreated
R18440 T23011 T23010 prep for,dried
R18441 T23012 T23013 nummod 30,min
R18442 T23013 T23011 pobj min,for
R18443 T23014 T22998 punct .,transferred
R18444 T23015 T23017 nsubjpass Blots,blocked
R18445 T23016 T23017 auxpass were,blocked
R18446 T23017 T23017 ROOT blocked,blocked
R18447 T23018 T23017 prep in,blocked
R18448 T23019 T23020 nummod 5,%
R18449 T23020 T23023 compound %,albumin
R18450 T23021 T23022 amod bovine,serum
R18451 T23022 T23023 compound serum,albumin
R18452 T23023 T23018 pobj albumin,in
R18453 T23024 T23017 prep in,blocked
R18454 T23025 T23026 nummod 0.1,%
R18455 T23026 T23024 pobj %,in
R18456 T23027 T23028 nsubj Tris,buffered
R18457 T23028 T23017 advcl buffered,blocked
R18458 T23029 T23028 dobj saline-Tween,buffered
R18459 T23030 T23029 nummod 20,saline-Tween
R18460 T23031 T23032 punct (,TBS-T
R18461 T23032 T23036 nsubj TBS-T,probed
R18462 T23033 T23032 punct ),TBS-T
R18463 T23034 T23032 punct ",",TBS-T
R18464 T23035 T23032 cc and,TBS-T
R18465 T23036 T23036 ROOT probed,probed
R18466 T23037 T23036 prep with,probed
R18467 T23038 T23039 amod specific,antibodies
R18468 T23039 T23037 pobj antibodies,with
R18469 T23040 T23041 mark as,described
R18470 T23041 T23042 amod described,previously46
R18471 T23042 T23036 dobj previously46,probed
R18472 T23043 T23036 punct .,probed
R18473 T23044 T23046 nsubjpass Bands,imaged
R18474 T23045 T23046 auxpass were,imaged
R18475 T23046 T23046 ROOT imaged,imaged
R18476 T23047 T23046 agent by,imaged
R18477 T23048 T23051 det the,system
R18478 T23049 T23051 compound Super,system
R18479 T23050 T23051 compound Signaling,system
R18480 T23051 T23047 pobj system,by
R18481 T23052 T23051 punct (,system
R18482 T23053 T23051 appos Pierce,system
R18483 T23054 T23051 punct ),system
R18484 T23055 T23046 prep according,imaged
R18485 T23056 T23055 prep to,according
R18486 T23057 T23058 det the,manufacturer
R18487 T23058 T23060 poss manufacturer,instructions
R18488 T23059 T23058 case 's,manufacturer
R18489 T23060 T23056 pobj instructions,to
R18490 T23061 T23046 punct .,imaged

UBERON-AE

Id Subject Object Predicate Lexical cue
T22539 863-867 http://purl.obolibrary.org/obo/UBERON_0001913 denotes milk
T22540 1161-1166 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

GO-BP

Id Subject Object Predicate Lexical cue
T23071 298-309 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T23072 722-727 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis

GO-MF

Id Subject Object Predicate Lexical cue
T23073 298-309 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T23074 576-584 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T23075 920-930 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T23076 1248-1258 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies

GO-CC

Id Subject Object Predicate Lexical cue
T23077 39-44 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T23078 576-584 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T23079 920-930 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T23080 1248-1258 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T23081 576-584 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T23082 920-930 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T23083 1248-1258 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T23084 826-835 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T23085 1070-1079 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes

sentences

Id Subject Object Predicate Lexical cue
T22542 0-34 Sentence denotes Immunoprecipitation and immunoblot
T22543 35-364 Sentence denotes The cells were harvested and lysed on ice by 0.4 ml of the modified RIPA buffer (50 mM Tris-HCl [pH 7.4], 1% NP-40, 0.25% Na-deoxycholate, 150 mM NaCl, 1 mM EDTA, 1 mM PMSF, 1 mM Na3VO4, 1 mM NaF) supplemented with 1 × protease inhibitor cocktail (Roche) and 1 × phosphatase inhibitor cocktail set I (EMD Biosciences) for 30 min.
T22544 365-456 Sentence denotes The lysates were centrifuged at 10,000 × g at 4 °C for 10 min to remove insoluble material.
T22545 457-810 Sentence denotes After normalizing protein concentrations, lysates were subjected to immunoprecipitation by adding 10 mg/ml appropriate antibody plus 30 ml of protein G-agarose (Roche), and rotated for at least 2 h at 4°C. The precipitates were washed at least five times with cold lysis buffer followed by separation by SDS-PAGE under reduced and denaturing conditions.
T22546 811-956 Sentence denotes Nitrocellulose membranes were blocked in 5 % nonfat milk in 0.1 % PBS-Tween 20 (PBS-T), probed with specific antibodies as described previously6.
T22547 957-1127 Sentence denotes For immunoblotting of phosphorylated proteins, gels were transferred to methanol-treated polyvinylidene chloride membranes, retreated with methanol, and dried for 30 min.
T22548 1128-1285 Sentence denotes Blots were blocked in 5 % bovine serum albumin in 0.1 % Tris buffered saline-Tween 20 (TBS-T), and probed with specific antibodies as described previously46.
T22549 1286-1388 Sentence denotes Bands were imaged by the Super Signaling system (Pierce) according to the manufacturer's instructions.
T239 0-34 Sentence denotes Immunoprecipitation and immunoblot
T240 35-364 Sentence denotes The cells were harvested and lysed on ice by 0.4 ml of the modified RIPA buffer (50 mM Tris-HCl [pH 7.4], 1% NP-40, 0.25% Na-deoxycholate, 150 mM NaCl, 1 mM EDTA, 1 mM PMSF, 1 mM Na3VO4, 1 mM NaF) supplemented with 1 × protease inhibitor cocktail (Roche) and 1 × phosphatase inhibitor cocktail set I (EMD Biosciences) for 30 min.
T241 365-456 Sentence denotes The lysates were centrifuged at 10,000 × g at 4 °C for 10 min to remove insoluble material.
T242 457-662 Sentence denotes After normalizing protein concentrations, lysates were subjected to immunoprecipitation by adding 10 mg/ml appropriate antibody plus 30 ml of protein G-agarose (Roche), and rotated for at least 2 h at 4°C.
T243 663-810 Sentence denotes The precipitates were washed at least five times with cold lysis buffer followed by separation by SDS-PAGE under reduced and denaturing conditions.
T244 811-956 Sentence denotes Nitrocellulose membranes were blocked in 5 % nonfat milk in 0.1 % PBS-Tween 20 (PBS-T), probed with specific antibodies as described previously6.
T245 957-1127 Sentence denotes For immunoblotting of phosphorylated proteins, gels were transferred to methanol-treated polyvinylidene chloride membranes, retreated with methanol, and dried for 30 min.
T246 1128-1285 Sentence denotes Blots were blocked in 5 % bovine serum albumin in 0.1 % Tris buffered saline-Tween 20 (TBS-T), and probed with specific antibodies as described previously46.
T247 1286-1388 Sentence denotes Bands were imaged by the Super Signaling system (Pierce) according to the manufacturer's instructions.

events-check-again

Id Subject Object Predicate Lexical cue
T23092 1167-1174 Protein denotes albumin

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T23086 224-230 Protein denotes mM NaF
T23087 294-309 Protein denotes 1 × phosphatase
T23088 1161-1174 Protein denotes serum albumin

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T22541 1167-1174 Protein denotes albumin

test2

Id Subject Object Predicate Lexical cue
T22538 1167-1174 Protein denotes albumin