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    {"project":"2_test","denotations":[{"id":"20478826-19727398-76650498","span":{"begin":685,"end":687},"obj":"19727398"}],"text":"Figure 2. Type IV secretion systems in plasticity zones. (A) Gene arrangement in PZ1 and comparison with corresponding regions in other genome sequences. PZ1 of strain P12 is inserted into a restriction–modification system pseudogene (similar to gene hp464 in strain 26695). Genes encoding type IV secretion system components are indicated as black arrows, and frameshift mutations are indicated by asterisks. Genes encoding proteins with 90–95% sequence similarity to the P12 proteins are shown in full colour, genes encoding proteins with 50–75% sequence similarity are hatched. Note that tfs4 has been termed tfs3a (for strains Shi470 and G27) or tfs3b (for strain P12) previously (32). PeCan18B plasticity zone, GenBank accession AF487344.3. (B) Neighbor-joining tree showing relationships between PZ type IV secretion systems and other Helicobacter and Campylobacter type IV secretion systems, based on average distances of the corresponding VirB4, VirB9 and VirB10 homologs. The TFS4 systems of strains P12 (tfs4-HPP12), G27 (tfs4-G27) and Shi470 (tfs4-HPSH) are depicted individually to show their mutual relationships. pTet, C. jejuni 81–176 pTet plasmid; pVir, C. jejuni 81–176 pVir plasmid."}