PMC:2948432 / 4469-5408
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2948432","sourcedb":"PMC","sourceid":"2948432","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2948432","text":"Most melanoma cell lines show deletion/mutation of CDKN2A (Flores et al.,1996; Walker et al.,1998). The majority of primary tumors have allelic loss at microsatellite markers mapping to the CDKN2A locus, indicating that deletions are the principal genetic event in vivo (Flores et al.,1996; Rodolfo et al.,2004). More recent reports showed biallelic deletion in ∼45% of melanoma metastases, supporting the role of this locus in melanoma progression (Grafstrom et al.,2005) and we have shown that epigenetic silencing of P14ARF is also common in metastatic disease (Freedberg et al.,2008). There are few data from primary tumors on the role of deletion at the locus on outcome (Koynova et al.,2007) and none on the effect of deletion across the larger region, which we have addressed using Multiplex ligation-dependent probe amplification (MLPA) (Nygren et al.,2005) rather than by studying the small intragenic regions previously reported.","tracks":[{"project":"2_test","denotations":[{"id":"20140953-8895759-43951714","span":{"begin":73,"end":77},"obj":"8895759"},{"id":"20140953-9598804-43951715","span":{"begin":93,"end":97},"obj":"9598804"},{"id":"20140953-8895759-43951716","span":{"begin":285,"end":289},"obj":"8895759"},{"id":"20140953-15363539-43951717","span":{"begin":306,"end":310},"obj":"15363539"},{"id":"20140953-15837753-43951718","span":{"begin":467,"end":471},"obj":"15837753"},{"id":"20140953-18505964-43951719","span":{"begin":582,"end":586},"obj":"18505964"},{"id":"20140953-16977458-43951720","span":{"begin":692,"end":696},"obj":"16977458"},{"id":"20140953-16106041-43951721","span":{"begin":860,"end":864},"obj":"16106041"}],"attributes":[{"subj":"20140953-8895759-43951714","pred":"source","obj":"2_test"},{"subj":"20140953-9598804-43951715","pred":"source","obj":"2_test"},{"subj":"20140953-8895759-43951716","pred":"source","obj":"2_test"},{"subj":"20140953-15363539-43951717","pred":"source","obj":"2_test"},{"subj":"20140953-15837753-43951718","pred":"source","obj":"2_test"},{"subj":"20140953-18505964-43951719","pred":"source","obj":"2_test"},{"subj":"20140953-16977458-43951720","pred":"source","obj":"2_test"},{"subj":"20140953-16106041-43951721","pred":"source","obj":"2_test"}]},{"project":"0_colil","denotations":[{"id":"20140953-8895759-957366","span":{"begin":73,"end":77},"obj":"8895759"},{"id":"20140953-9598804-957367","span":{"begin":93,"end":97},"obj":"9598804"},{"id":"20140953-8895759-957368","span":{"begin":285,"end":289},"obj":"8895759"},{"id":"20140953-15363539-957369","span":{"begin":306,"end":310},"obj":"15363539"},{"id":"20140953-15837753-957370","span":{"begin":467,"end":471},"obj":"15837753"},{"id":"20140953-18505964-957371","span":{"begin":582,"end":586},"obj":"18505964"},{"id":"20140953-16977458-957372","span":{"begin":692,"end":696},"obj":"16977458"},{"id":"20140953-16106041-957373","span":{"begin":860,"end":864},"obj":"16106041"}],"attributes":[{"subj":"20140953-8895759-957366","pred":"source","obj":"0_colil"},{"subj":"20140953-9598804-957367","pred":"source","obj":"0_colil"},{"subj":"20140953-8895759-957368","pred":"source","obj":"0_colil"},{"subj":"20140953-15363539-957369","pred":"source","obj":"0_colil"},{"subj":"20140953-15837753-957370","pred":"source","obj":"0_colil"},{"subj":"20140953-18505964-957371","pred":"source","obj":"0_colil"},{"subj":"20140953-16977458-957372","pred":"source","obj":"0_colil"},{"subj":"20140953-16106041-957373","pred":"source","obj":"0_colil"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#9393ec","default":true},{"id":"0_colil","color":"#adec93"}]}]}}