PMC:2944628 / 11275-12186 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2944628","sourcedb":"PMC","sourceid":"2944628","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2944628","text":"Immunopositive cells (DCX and BrdU) were counted in a defined region of hippocampus. Specifically, cells were counted if they resided in the subgranular zone (SGZ) of the dentate gyrus and in granule cell layer (GCL). The SGZ was defined as a two-cell width wide (approximately 20 μm) region just below the GCL. All cells within each section were counted, but excluding top and bottom surfaces of the sections in order to avoid counting cells that were dissected or damaged. The average number of cells per section was multiplied by the total number of sections to obtain total cell numbers per DG. In some cases, where we compare dorsal vs. ventral regions, we also report average cell densities per section for each region. Double-labeling (DCX/BrdU and CaBP/BrdU) was identified using a Leica TCS-SL confocal microscope (Leica Microsystems (Canada) Inc.; Richmond Hill, ON, Canada) with a 63× objective lens.","tracks":[]}