PMC:2940544 / 8839-9931 JSONTXT

{"project":"TEST0","denotations":[{"id":"20859526-120-128-568136","span":{"begin":120,"end":124},"obj":"[\"17965719\"]"},{"id":"20859526-227-235-568137","span":{"begin":755,"end":759},"obj":"[\"18539336\"]"},{"id":"20859526-233-241-568138","span":{"begin":904,"end":908},"obj":"[\"17898141\"]"},{"id":"20859526-118-126-568139","span":{"begin":1086,"end":1090},"obj":"[\"17143280\"]"}],"text":"A different strategy is to only scan the points of interest rather than all of the pixels in a frame (Nikolenko et al., 2007). In this method the galvanometer mirror is commanded to jump from one point of interest (i.e., cell body) to another in series until all points of interest are sampled and then repeat the sampling cycle – each cycle representing a “frame”. With relatively small numbers of points of interest this can allow for very fast scans, though the method slows down significantly with larger numbers of points. This method has been optimized in recent work wherein the momentum of the scan mirrors was taken into account such that scanning either occurs with maximal optimized acceleration and deceleration between points (Lillis et al., 2008) or as a single smooth movement at constant speed passing through all of the necessary points of interest without stopping (Gobel and Helmchen, 2007), thereby improving the efficiency of the method yet more. These authors later expanded this method into the third dimension using a Z-scanning objective adaptor (Gobel et al., 2007)."}

{"project":"0_colil","denotations":[{"id":"20859526-17965719-568136","span":{"begin":120,"end":124},"obj":"17965719"},{"id":"20859526-18539336-568137","span":{"begin":755,"end":759},"obj":"18539336"},{"id":"20859526-17898141-568138","span":{"begin":904,"end":908},"obj":"17898141"},{"id":"20859526-17143280-568139","span":{"begin":1086,"end":1090},"obj":"17143280"}],"text":"A different strategy is to only scan the points of interest rather than all of the pixels in a frame (Nikolenko et al., 2007). In this method the galvanometer mirror is commanded to jump from one point of interest (i.e., cell body) to another in series until all points of interest are sampled and then repeat the sampling cycle – each cycle representing a “frame”. With relatively small numbers of points of interest this can allow for very fast scans, though the method slows down significantly with larger numbers of points. This method has been optimized in recent work wherein the momentum of the scan mirrors was taken into account such that scanning either occurs with maximal optimized acceleration and deceleration between points (Lillis et al., 2008) or as a single smooth movement at constant speed passing through all of the necessary points of interest without stopping (Gobel and Helmchen, 2007), thereby improving the efficiency of the method yet more. These authors later expanded this method into the third dimension using a Z-scanning objective adaptor (Gobel et al., 2007)."}

{"project":"2_test","denotations":[{"id":"20859526-17965719-38464478","span":{"begin":120,"end":124},"obj":"17965719"},{"id":"20859526-18539336-38464479","span":{"begin":755,"end":759},"obj":"18539336"},{"id":"20859526-17898141-38464480","span":{"begin":904,"end":908},"obj":"17898141"},{"id":"20859526-17143280-38464481","span":{"begin":1086,"end":1090},"obj":"17143280"}],"text":"A different strategy is to only scan the points of interest rather than all of the pixels in a frame (Nikolenko et al., 2007). In this method the galvanometer mirror is commanded to jump from one point of interest (i.e., cell body) to another in series until all points of interest are sampled and then repeat the sampling cycle – each cycle representing a “frame”. With relatively small numbers of points of interest this can allow for very fast scans, though the method slows down significantly with larger numbers of points. This method has been optimized in recent work wherein the momentum of the scan mirrors was taken into account such that scanning either occurs with maximal optimized acceleration and deceleration between points (Lillis et al., 2008) or as a single smooth movement at constant speed passing through all of the necessary points of interest without stopping (Gobel and Helmchen, 2007), thereby improving the efficiency of the method yet more. These authors later expanded this method into the third dimension using a Z-scanning objective adaptor (Gobel et al., 2007)."}