PMC:2940544 / 7423-8814
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2940544","sourcedb":"PMC","sourceid":"2940544","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2940544","text":"Enhanced scanning methods\nWe and others have concentrated on optimizing the scanning aspects of two-photon microscopy. Traditionally “frames” in a time lapse movie were created by scanning the focused laser across the microscopic field in a series of parallel lines. The scanning occurred by control of the angle of the laser path using galvanometer mirrors which pivot about fixed axes. Resonant mirror systems have gained some favor since they allow for very high speed (up to several KHz) scanning in one axis for faster scanning of each line in the image (Tsien, 1995). This technology is based on galvanometer mirrors that resonantly sweep back-and-forth at a fixed frequency when activated and can produce very nice full frame scanning results (Rochefort et al., 2009), though it does not allow for flexibility in how the scanners are used, restricting at least one axis to linear back-and-forth scanning. Another fast scanning method utilizes acousto-optical devices which control the beam path not with mirrors but with crystals whose refractive properties are very quickly altered by varying sound waves passing through them (Goldstein et al., 1990). These devices allow for flexible fast scanning and can be optimized to produce very useful results (Otsu et al., 2008), but have the limitation that only a narrow range of angles can be scanned without degradation of image quality.","divisions":[{"label":"title","span":{"begin":0,"end":25}}],"tracks":[{"project":"TEST0","denotations":[{"id":"20859526-195-203-568133","span":{"begin":769,"end":773},"obj":"[\"19706480\"]"},{"id":"20859526-233-241-568134","span":{"begin":1153,"end":1157},"obj":"[\"2299661\"]"},{"id":"20859526-113-121-568135","span":{"begin":1273,"end":1277},"obj":"[\"18634822\"]"}],"attributes":[{"subj":"20859526-195-203-568133","pred":"source","obj":"TEST0"},{"subj":"20859526-233-241-568134","pred":"source","obj":"TEST0"},{"subj":"20859526-113-121-568135","pred":"source","obj":"TEST0"}]},{"project":"0_colil","denotations":[{"id":"20859526-19706480-568133","span":{"begin":769,"end":773},"obj":"19706480"},{"id":"20859526-2299661-568134","span":{"begin":1153,"end":1157},"obj":"2299661"},{"id":"20859526-18634822-568135","span":{"begin":1273,"end":1277},"obj":"18634822"}],"attributes":[{"subj":"20859526-19706480-568133","pred":"source","obj":"0_colil"},{"subj":"20859526-2299661-568134","pred":"source","obj":"0_colil"},{"subj":"20859526-18634822-568135","pred":"source","obj":"0_colil"}]},{"project":"2_test","denotations":[{"id":"20859526-19706480-38464475","span":{"begin":769,"end":773},"obj":"19706480"},{"id":"20859526-2299661-38464476","span":{"begin":1153,"end":1157},"obj":"2299661"},{"id":"20859526-18634822-38464477","span":{"begin":1273,"end":1277},"obj":"18634822"}],"attributes":[{"subj":"20859526-19706480-38464475","pred":"source","obj":"2_test"},{"subj":"20859526-2299661-38464476","pred":"source","obj":"2_test"},{"subj":"20859526-18634822-38464477","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"TEST0","color":"#93d8ec","default":true},{"id":"0_colil","color":"#ece693"},{"id":"2_test","color":"#cc93ec"}]}]}}