PMC:2940544 / 23247-24291 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2940544","sourcedb":"PMC","sourceid":"2940544","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2940544","text":"Imaging-based uses of the SLM include simultaneous time lapse imaging of a fixed set of multiple targets. This is accomplished by delivering excitation light to all targets continuously while monitoring emission at all those sites at once using a camera. The number of excitation points which may be specified and still receive sufficient excitation power to give good signals is currently limited by the amount of power produced by modern lasers (roughly 20 neurons in brain slice experiments). Liquid crystal SLMs can update their output pattern every 16.7 ms (or more often, depending on the model of SLM) for faster than video rate updating of projected patterns. This temporal flexibility means the SLM may also be useful as a multiplexed random access scanner. In this mode it would update to a new set of multiple targets (rather than a single target) every 16.7 ms, thus allowing allow for random access scanning across large numbers of targets. This may be of particular interest in neuronal integration or circuit integration studies.","tracks":[]}