PMC:2940544 / 20015-21595
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2940544","sourcedb":"PMC","sourceid":"2940544","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2940544","text":"We and others have developed another multibeam approach which uses SLMs in one-photon (Lutz et al., 2008) and two-photon regimes (Nikolenko et al., 2008). SLMs not only allow for truly simultaneous monitoring of fluorescence at multiple targets, but also allow for simultaneous photomanipulation of targets in a customizable and temporally dynamic manner. The core of this method is the SLM itself which acts as a programmable DOE to construct a custom light or beamlet pattern (Figures 3A,B), and can in fact dynamically change that pattern over time (at 60 Hz in our case). SLMs can create any pattern of light within the output resolution of the SLM itself (256 × 256 in our case) since they are made of dynamic controllable materials such as liquid crystal which allow the diffractive properties of each “pixel” of the SLM to be modified by electrical signals from a computer. It is important that as opposed to micromirror technology (Wang et al., 2007) in which patterns can be created by blocking out light at individual pixels to varying degrees (a purely subtractive process), the phase-only SLM (and DOE) preserves incident light by creating both destructive and constructive interference. For example, a beamlet pattern on a 256 × 256 output image in which only three pixels are allowed to be bright (i.e., three beamlets) keeps 50% of the input power in the output pattern, versus \u003c1% with micromirror devices. An SLM approach was developed for one-photon microscopy by (Lutz et al., 2008), in parallel to the two-photon approach we developed (Nikolenko et al., 2008).","tracks":[{"project":"TEST0","denotations":[{"id":"20859526-100-108-568153","span":{"begin":100,"end":104},"obj":"[\"19160517\"]"},{"id":"20859526-148-156-568154","span":{"begin":148,"end":152},"obj":"[\"19129923\"]"},{"id":"20859526-72-80-568155","span":{"begin":953,"end":957},"obj":"[\"18034506\"]"},{"id":"20859526-73-81-568156","span":{"begin":1496,"end":1500},"obj":"[\"19160517\"]"},{"id":"20859526-151-159-568157","span":{"begin":1574,"end":1578},"obj":"[\"19129923\"]"}],"attributes":[{"subj":"20859526-100-108-568153","pred":"source","obj":"TEST0"},{"subj":"20859526-148-156-568154","pred":"source","obj":"TEST0"},{"subj":"20859526-72-80-568155","pred":"source","obj":"TEST0"},{"subj":"20859526-73-81-568156","pred":"source","obj":"TEST0"},{"subj":"20859526-151-159-568157","pred":"source","obj":"TEST0"}]},{"project":"0_colil","denotations":[{"id":"20859526-19160517-568153","span":{"begin":100,"end":104},"obj":"19160517"},{"id":"20859526-19129923-568154","span":{"begin":148,"end":152},"obj":"19129923"},{"id":"20859526-18034506-568155","span":{"begin":953,"end":957},"obj":"18034506"},{"id":"20859526-19160517-568156","span":{"begin":1496,"end":1500},"obj":"19160517"},{"id":"20859526-19129923-568157","span":{"begin":1574,"end":1578},"obj":"19129923"}],"attributes":[{"subj":"20859526-19160517-568153","pred":"source","obj":"0_colil"},{"subj":"20859526-19129923-568154","pred":"source","obj":"0_colil"},{"subj":"20859526-18034506-568155","pred":"source","obj":"0_colil"},{"subj":"20859526-19160517-568156","pred":"source","obj":"0_colil"},{"subj":"20859526-19129923-568157","pred":"source","obj":"0_colil"}]},{"project":"2_test","denotations":[{"id":"20859526-19160517-38464495","span":{"begin":100,"end":104},"obj":"19160517"},{"id":"20859526-19129923-38464496","span":{"begin":148,"end":152},"obj":"19129923"},{"id":"20859526-18034506-38464497","span":{"begin":953,"end":957},"obj":"18034506"},{"id":"20859526-19160517-38464498","span":{"begin":1496,"end":1500},"obj":"19160517"},{"id":"20859526-19129923-38464499","span":{"begin":1574,"end":1578},"obj":"19129923"}],"attributes":[{"subj":"20859526-19160517-38464495","pred":"source","obj":"2_test"},{"subj":"20859526-19129923-38464496","pred":"source","obj":"2_test"},{"subj":"20859526-18034506-38464497","pred":"source","obj":"2_test"},{"subj":"20859526-19160517-38464498","pred":"source","obj":"2_test"},{"subj":"20859526-19129923-38464499","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"TEST0","color":"#ecdf93","default":true},{"id":"0_colil","color":"#c593ec"},{"id":"2_test","color":"#93ecab"}]}]}}