PMC:2940544 / 17045-18185 JSONTXT

{"project":"TEST0","denotations":[{"id":"20859526-76-84-568151","span":{"begin":76,"end":80},"obj":"[\"19636390\"]"}],"text":"A second use of the DOE is one we have called “speed boost” (Watson et al., 2009). This is a method for making full frame scans by using beamlets spread equidistantly over the entire frame. These beamlets each scan a fraction or stripe of the field of interest, and by doing so simultaneously will quickly cover the full field. n equally spaced beamlets can thereby scan a field in roughly 1/n the time needed to scan with a single beam. This method requires only small modification of existing scanning setups: (1) setting up the DOE and (2) setting up an imaging device which spatially resolves light coming from many sources at once such as a camera or multi-anode PMT (without modification of scanning software). Using this method we were able to create high quality images at video rate with good signal-to-noise ratios. We were able to very effectively detect action potential-related calcium fluxes in neurons at high speed as shown in Figure 2. While this strategy is inexpensive and easily combined with other techniques it is reliant on large amounts of laser power and is only useful in situations where extra power is available."}

{"project":"0_colil","denotations":[{"id":"20859526-19636390-568151","span":{"begin":76,"end":80},"obj":"19636390"}],"text":"A second use of the DOE is one we have called “speed boost” (Watson et al., 2009). This is a method for making full frame scans by using beamlets spread equidistantly over the entire frame. These beamlets each scan a fraction or stripe of the field of interest, and by doing so simultaneously will quickly cover the full field. n equally spaced beamlets can thereby scan a field in roughly 1/n the time needed to scan with a single beam. This method requires only small modification of existing scanning setups: (1) setting up the DOE and (2) setting up an imaging device which spatially resolves light coming from many sources at once such as a camera or multi-anode PMT (without modification of scanning software). Using this method we were able to create high quality images at video rate with good signal-to-noise ratios. We were able to very effectively detect action potential-related calcium fluxes in neurons at high speed as shown in Figure 2. While this strategy is inexpensive and easily combined with other techniques it is reliant on large amounts of laser power and is only useful in situations where extra power is available."}

{"project":"2_test","denotations":[{"id":"20859526-19636390-38464493","span":{"begin":76,"end":80},"obj":"19636390"}],"text":"A second use of the DOE is one we have called “speed boost” (Watson et al., 2009). This is a method for making full frame scans by using beamlets spread equidistantly over the entire frame. These beamlets each scan a fraction or stripe of the field of interest, and by doing so simultaneously will quickly cover the full field. n equally spaced beamlets can thereby scan a field in roughly 1/n the time needed to scan with a single beam. This method requires only small modification of existing scanning setups: (1) setting up the DOE and (2) setting up an imaging device which spatially resolves light coming from many sources at once such as a camera or multi-anode PMT (without modification of scanning software). Using this method we were able to create high quality images at video rate with good signal-to-noise ratios. We were able to very effectively detect action potential-related calcium fluxes in neurons at high speed as shown in Figure 2. While this strategy is inexpensive and easily combined with other techniques it is reliant on large amounts of laser power and is only useful in situations where extra power is available."}