PMC:2889865 / 8630-10742 JSON TXT

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pmc-enju-pas

bionlp-st-ge-2016-coref

{"project":"bionlp-st-ge-2016-coref","denotations":[{"id":"T4372","span":{"begin":990,"end":1003},"obj":"Anaphor"},{"id":"T4373","span":{"begin":859,"end":864},"obj":"Antecedent"},{"id":"T4374","span":{"begin":915,"end":919},"obj":"Antecedent"}],"relations":[{"id":"R3611","pred":"boundBy","subj":"T4372","obj":"T4373"},{"id":"R3612","pred":"boundBy","subj":"T4372","obj":"T4374"}],"namespaces":[{"prefix":"_base","uri":"https://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Induction of inflammatory responses\nThe ability of PMA and HK E. coli to induce an inflammatory response in Jurkat T-cells was evaluated using a multiplex cytokine assay following 24 h stimulation. The cytokine profile revealed an enhanced induction of the pro-inflammatory cytokines IL-2, IL-6, TNF and the chemokine CXCL8. The levels of the anti-inflammatory cytokine IL-10 were unaffected by PMA but were significantly decreased by HK E. coli (Table 1). These results confirmed that PMA and HK E. coli induced an inflammatory response in the Jurkat T-cells. It is interesting to note that PMA was 120-fold more effective at inducing CXCL8 than HK E. coli. PMA-dependent induction of AP-1 and down-regulation of NF-κB suggests an involvement of AP-1 in CXCL8 regulation. Determination of the time course of cytokine induction in response to PMA showed that CXCL8 was already released between 2-6 h, while TNF and IL-6 were released between 6-24 h (figure 3). These results indicated that the cytokines were differentially regulated and that the release was not associated with the early transient induction of NF-κB. The temporal induction of AP-1 correlated to the CXCL8 levels and preceded the TNF and IL-6 release. This suggests an association between CXCL8 release and AP-1 signalling.\nFigure 3 CXCL8, IL-6 and TNF expression following long-term stimulation with PMA. Jurkat T-cells were either treated with media (white bars) or stimulated with PMA (black bars) and incubated for 1 h, 2 h, 6 h and 24 h. Aged media was added following each centrifugation step representative to the stimulation time (see Materials and methods). Cytokine levels (A) CXCL8, (B) IL-6 and (C) TNF were detected by ELISA. Statistical significance from the control was determined using Student's t-test. (n = 3).\nTable 1 Jurkat T-cells were stimulated with 162 nM PMA and 5 × 107 CFU/ml HK E. coli for 24 h. Cytokine levels were determined using multiplex assays. Concentrations are given as pg/ml for each group (mean value ± SD, n = 3). Statistical significance from the control (C) was determined using Student's t-test.\n\n"}

bionlp-st-ge-2016-spacy-parsed

GO-BP

{"project":"GO-BP","denotations":[{"id":"T4404","span":{"begin":13,"end":35},"obj":"http://purl.obolibrary.org/obo/GO_0006954"},{"id":"T4405","span":{"begin":83,"end":104},"obj":"http://purl.obolibrary.org/obo/GO_0006954"},{"id":"T4406","span":{"begin":516,"end":537},"obj":"http://purl.obolibrary.org/obo/GO_0006954"},{"id":"T4407","span":{"begin":700,"end":710},"obj":"http://purl.obolibrary.org/obo/GO_0065007"},{"id":"T4408","span":{"begin":761,"end":771},"obj":"http://purl.obolibrary.org/obo/GO_0065007"},{"id":"T4409","span":{"begin":809,"end":827},"obj":"http://purl.obolibrary.org/obo/GO_0042089"},{"id":"T4410","span":{"begin":831,"end":846},"obj":"http://purl.obolibrary.org/obo/GO_1904627"},{"id":"T4411","span":{"begin":1280,"end":1290},"obj":"http://purl.obolibrary.org/obo/GO_0023052"}],"text":"Induction of inflammatory responses\nThe ability of PMA and HK E. coli to induce an inflammatory response in Jurkat T-cells was evaluated using a multiplex cytokine assay following 24 h stimulation. The cytokine profile revealed an enhanced induction of the pro-inflammatory cytokines IL-2, IL-6, TNF and the chemokine CXCL8. The levels of the anti-inflammatory cytokine IL-10 were unaffected by PMA but were significantly decreased by HK E. coli (Table 1). These results confirmed that PMA and HK E. coli induced an inflammatory response in the Jurkat T-cells. It is interesting to note that PMA was 120-fold more effective at inducing CXCL8 than HK E. coli. PMA-dependent induction of AP-1 and down-regulation of NF-κB suggests an involvement of AP-1 in CXCL8 regulation. Determination of the time course of cytokine induction in response to PMA showed that CXCL8 was already released between 2-6 h, while TNF and IL-6 were released between 6-24 h (figure 3). These results indicated that the cytokines were differentially regulated and that the release was not associated with the early transient induction of NF-κB. The temporal induction of AP-1 correlated to the CXCL8 levels and preceded the TNF and IL-6 release. This suggests an association between CXCL8 release and AP-1 signalling.\nFigure 3 CXCL8, IL-6 and TNF expression following long-term stimulation with PMA. Jurkat T-cells were either treated with media (white bars) or stimulated with PMA (black bars) and incubated for 1 h, 2 h, 6 h and 24 h. Aged media was added following each centrifugation step representative to the stimulation time (see Materials and methods). Cytokine levels (A) CXCL8, (B) IL-6 and (C) TNF were detected by ELISA. Statistical significance from the control was determined using Student's t-test. (n = 3).\nTable 1 Jurkat T-cells were stimulated with 162 nM PMA and 5 × 107 CFU/ml HK E. coli for 24 h. Cytokine levels were determined using multiplex assays. Concentrations are given as pg/ml for each group (mean value ± SD, n = 3). Statistical significance from the control (C) was determined using Student's t-test.\n\n"}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T4412","span":{"begin":284,"end":288},"obj":"http://purl.obolibrary.org/obo/GO_0005134"},{"id":"T4413","span":{"begin":290,"end":294},"obj":"http://purl.obolibrary.org/obo/GO_0005138"},{"id":"T4414","span":{"begin":915,"end":919},"obj":"http://purl.obolibrary.org/obo/GO_0005138"},{"id":"T4415","span":{"begin":1206,"end":1210},"obj":"http://purl.obolibrary.org/obo/GO_0005138"},{"id":"T4416","span":{"begin":370,"end":375},"obj":"http://purl.obolibrary.org/obo/GO_0005141"},{"id":"T15814","span":{"begin":1309,"end":1313},"obj":"http://purl.obolibrary.org/obo/GO_0005138"},{"id":"T15815","span":{"begin":1667,"end":1671},"obj":"http://purl.obolibrary.org/obo/GO_0005138"}],"text":"Induction of inflammatory responses\nThe ability of PMA and HK E. coli to induce an inflammatory response in Jurkat T-cells was evaluated using a multiplex cytokine assay following 24 h stimulation. The cytokine profile revealed an enhanced induction of the pro-inflammatory cytokines IL-2, IL-6, TNF and the chemokine CXCL8. The levels of the anti-inflammatory cytokine IL-10 were unaffected by PMA but were significantly decreased by HK E. coli (Table 1). These results confirmed that PMA and HK E. coli induced an inflammatory response in the Jurkat T-cells. It is interesting to note that PMA was 120-fold more effective at inducing CXCL8 than HK E. coli. PMA-dependent induction of AP-1 and down-regulation of NF-κB suggests an involvement of AP-1 in CXCL8 regulation. Determination of the time course of cytokine induction in response to PMA showed that CXCL8 was already released between 2-6 h, while TNF and IL-6 were released between 6-24 h (figure 3). These results indicated that the cytokines were differentially regulated and that the release was not associated with the early transient induction of NF-κB. The temporal induction of AP-1 correlated to the CXCL8 levels and preceded the TNF and IL-6 release. This suggests an association between CXCL8 release and AP-1 signalling.\nFigure 3 CXCL8, IL-6 and TNF expression following long-term stimulation with PMA. Jurkat T-cells were either treated with media (white bars) or stimulated with PMA (black bars) and incubated for 1 h, 2 h, 6 h and 24 h. Aged media was added following each centrifugation step representative to the stimulation time (see Materials and methods). Cytokine levels (A) CXCL8, (B) IL-6 and (C) TNF were detected by ELISA. Statistical significance from the control was determined using Student's t-test. (n = 3).\nTable 1 Jurkat T-cells were stimulated with 162 nM PMA and 5 × 107 CFU/ml HK E. coli for 24 h. Cytokine levels were determined using multiplex assays. Concentrations are given as pg/ml for each group (mean value ± SD, n = 3). Statistical significance from the control (C) was determined using Student's t-test.\n\n"}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T4417","span":{"begin":117,"end":122},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T4418","span":{"begin":554,"end":559},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T15816","span":{"begin":1384,"end":1389},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T17045","span":{"begin":1816,"end":1821},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Induction of inflammatory responses\nThe ability of PMA and HK E. coli to induce an inflammatory response in Jurkat T-cells was evaluated using a multiplex cytokine assay following 24 h stimulation. The cytokine profile revealed an enhanced induction of the pro-inflammatory cytokines IL-2, IL-6, TNF and the chemokine CXCL8. The levels of the anti-inflammatory cytokine IL-10 were unaffected by PMA but were significantly decreased by HK E. coli (Table 1). These results confirmed that PMA and HK E. coli induced an inflammatory response in the Jurkat T-cells. It is interesting to note that PMA was 120-fold more effective at inducing CXCL8 than HK E. coli. PMA-dependent induction of AP-1 and down-regulation of NF-κB suggests an involvement of AP-1 in CXCL8 regulation. Determination of the time course of cytokine induction in response to PMA showed that CXCL8 was already released between 2-6 h, while TNF and IL-6 were released between 6-24 h (figure 3). These results indicated that the cytokines were differentially regulated and that the release was not associated with the early transient induction of NF-κB. The temporal induction of AP-1 correlated to the CXCL8 levels and preceded the TNF and IL-6 release. This suggests an association between CXCL8 release and AP-1 signalling.\nFigure 3 CXCL8, IL-6 and TNF expression following long-term stimulation with PMA. Jurkat T-cells were either treated with media (white bars) or stimulated with PMA (black bars) and incubated for 1 h, 2 h, 6 h and 24 h. Aged media was added following each centrifugation step representative to the stimulation time (see Materials and methods). Cytokine levels (A) CXCL8, (B) IL-6 and (C) TNF were detected by ELISA. Statistical significance from the control was determined using Student's t-test. (n = 3).\nTable 1 Jurkat T-cells were stimulated with 162 nM PMA and 5 × 107 CFU/ml HK E. coli for 24 h. Cytokine levels were determined using multiplex assays. Concentrations are given as pg/ml for each group (mean value ± SD, n = 3). Statistical significance from the control (C) was determined using Student's t-test.\n\n"}

sentences

events-check-again

bionlp-st-ge-2016-reference-tees

bionlp-st-ge-2016-reference

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T4375","span":{"begin":284,"end":288},"obj":"P60568"},{"id":"T4376","span":{"begin":290,"end":294},"obj":"P05231"},{"id":"T4377","span":{"begin":296,"end":299},"obj":"P01375"},{"id":"T4378","span":{"begin":318,"end":323},"obj":"P10145"},{"id":"T4379","span":{"begin":370,"end":375},"obj":"P22301"},{"id":"T4380","span":{"begin":636,"end":641},"obj":"P10145"},{"id":"T4381","span":{"begin":686,"end":690},"obj":"P05412"},{"id":"T4382","span":{"begin":747,"end":751},"obj":"P05412"},{"id":"T4383","span":{"begin":755,"end":760},"obj":"P10145"},{"id":"T4384","span":{"begin":859,"end":864},"obj":"P10145"},{"id":"T4385","span":{"begin":907,"end":910},"obj":"P01375"},{"id":"T4386","span":{"begin":915,"end":919},"obj":"P05231"},{"id":"T4387","span":{"begin":1145,"end":1149},"obj":"P05412"},{"id":"T4388","span":{"begin":1168,"end":1173},"obj":"P10145"},{"id":"T4389","span":{"begin":1198,"end":1201},"obj":"P01375"},{"id":"T4390","span":{"begin":1206,"end":1210},"obj":"P05231"},{"id":"T4391","span":{"begin":1257,"end":1262},"obj":"P10145"},{"id":"T4392","span":{"begin":1275,"end":1279},"obj":"P05412"},{"id":"T15803","span":{"begin":1302,"end":1307},"obj":"P10145"},{"id":"T15804","span":{"begin":1309,"end":1313},"obj":"P05231"},{"id":"T15805","span":{"begin":1318,"end":1321},"obj":"P01375"},{"id":"T15806","span":{"begin":1656,"end":1661},"obj":"P10145"},{"id":"T15807","span":{"begin":1667,"end":1671},"obj":"P05231"},{"id":"T15808","span":{"begin":1680,"end":1683},"obj":"P01375"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Induction of inflammatory responses\nThe ability of PMA and HK E. coli to induce an inflammatory response in Jurkat T-cells was evaluated using a multiplex cytokine assay following 24 h stimulation. The cytokine profile revealed an enhanced induction of the pro-inflammatory cytokines IL-2, IL-6, TNF and the chemokine CXCL8. The levels of the anti-inflammatory cytokine IL-10 were unaffected by PMA but were significantly decreased by HK E. coli (Table 1). These results confirmed that PMA and HK E. coli induced an inflammatory response in the Jurkat T-cells. It is interesting to note that PMA was 120-fold more effective at inducing CXCL8 than HK E. coli. PMA-dependent induction of AP-1 and down-regulation of NF-κB suggests an involvement of AP-1 in CXCL8 regulation. Determination of the time course of cytokine induction in response to PMA showed that CXCL8 was already released between 2-6 h, while TNF and IL-6 were released between 6-24 h (figure 3). These results indicated that the cytokines were differentially regulated and that the release was not associated with the early transient induction of NF-κB. The temporal induction of AP-1 correlated to the CXCL8 levels and preceded the TNF and IL-6 release. This suggests an association between CXCL8 release and AP-1 signalling.\nFigure 3 CXCL8, IL-6 and TNF expression following long-term stimulation with PMA. Jurkat T-cells were either treated with media (white bars) or stimulated with PMA (black bars) and incubated for 1 h, 2 h, 6 h and 24 h. Aged media was added following each centrifugation step representative to the stimulation time (see Materials and methods). Cytokine levels (A) CXCL8, (B) IL-6 and (C) TNF were detected by ELISA. Statistical significance from the control was determined using Student's t-test. (n = 3).\nTable 1 Jurkat T-cells were stimulated with 162 nM PMA and 5 × 107 CFU/ml HK E. coli for 24 h. Cytokine levels were determined using multiplex assays. Concentrations are given as pg/ml for each group (mean value ± SD, n = 3). Statistical significance from the control (C) was determined using Student's t-test.\n\n"}

test2

PMC:2889865 / 8630-10742 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:2889865 / 8630-10742 JSON TXT