PMC:2889865 / 28839-29367
Annnotations
pmc-enju-pas
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bionlp-st-ge-2016-spacy-parsed
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cytokine assay\nQuantification of the levels of cytokines IL-2, IL-6, IL-10 and TNF and the chemokine CXCL8 was performed on culture supernatants using multiplexed biomarker immunoassay kits according to manufacturer's instructions (Bio-Rad Laboratories, Hercules, CA). A Bio-Plex™ 200 readout System was used (Bio-Rad), which utilizes Luminex® xMAP™ fluorescent bead-based technology (Luminex Corp., Austin). Levels were automatically calculated from standard curves using Bio-Plex Manager software (v.4.1.1, Bio-Rad)."}
GO-BP
{"project":"GO-BP","denotations":[{"id":"T13394","span":{"begin":274,"end":276},"obj":"http://purl.obolibrary.org/obo/GO_0033968"}],"text":"Multiplex cytokine assay\nQuantification of the levels of cytokines IL-2, IL-6, IL-10 and TNF and the chemokine CXCL8 was performed on culture supernatants using multiplexed biomarker immunoassay kits according to manufacturer's instructions (Bio-Rad Laboratories, Hercules, CA). A Bio-Plex™ 200 readout System was used (Bio-Rad), which utilizes Luminex® xMAP™ fluorescent bead-based technology (Luminex Corp., Austin). Levels were automatically calculated from standard curves using Bio-Plex Manager software (v.4.1.1, Bio-Rad)."}
GO-MF
{"project":"GO-MF","denotations":[{"id":"T13395","span":{"begin":67,"end":71},"obj":"http://purl.obolibrary.org/obo/GO_0005134"},{"id":"T13396","span":{"begin":73,"end":77},"obj":"http://purl.obolibrary.org/obo/GO_0005138"},{"id":"T13397","span":{"begin":79,"end":84},"obj":"http://purl.obolibrary.org/obo/GO_0005141"},{"id":"T13398","span":{"begin":274,"end":276},"obj":"http://purl.obolibrary.org/obo/GO_0033968"}],"text":"Multiplex cytokine assay\nQuantification of the levels of cytokines IL-2, IL-6, IL-10 and TNF and the chemokine CXCL8 was performed on culture supernatants using multiplexed biomarker immunoassay kits according to manufacturer's instructions (Bio-Rad Laboratories, Hercules, CA). A Bio-Plex™ 200 readout System was used (Bio-Rad), which utilizes Luminex® xMAP™ fluorescent bead-based technology (Luminex Corp., Austin). Levels were automatically calculated from standard curves using Bio-Plex Manager software (v.4.1.1, Bio-Rad)."}
sentences
{"project":"sentences","denotations":[{"id":"T13208","span":{"begin":0,"end":24},"obj":"Sentence"},{"id":"T13209","span":{"begin":25,"end":278},"obj":"Sentence"},{"id":"T13210","span":{"begin":279,"end":418},"obj":"Sentence"},{"id":"T13211","span":{"begin":419,"end":528},"obj":"Sentence"},{"id":"T209","span":{"begin":0,"end":24},"obj":"Sentence"},{"id":"T210","span":{"begin":25,"end":278},"obj":"Sentence"},{"id":"T211","span":{"begin":279,"end":418},"obj":"Sentence"},{"id":"T212","span":{"begin":419,"end":528},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Multiplex cytokine assay\nQuantification of the levels of cytokines IL-2, IL-6, IL-10 and TNF and the chemokine CXCL8 was performed on culture supernatants using multiplexed biomarker immunoassay kits according to manufacturer's instructions (Bio-Rad Laboratories, Hercules, CA). A Bio-Plex™ 200 readout System was used (Bio-Rad), which utilizes Luminex® xMAP™ fluorescent bead-based technology (Luminex Corp., Austin). Levels were automatically calculated from standard curves using Bio-Plex Manager software (v.4.1.1, Bio-Rad)."}
events-check-again
{"project":"events-check-again","denotations":[{"id":"T13419","span":{"begin":67,"end":71},"obj":"Protein"},{"id":"T13420","span":{"begin":73,"end":77},"obj":"Protein"},{"id":"T13421","span":{"begin":79,"end":84},"obj":"Protein"},{"id":"T13422","span":{"begin":111,"end":116},"obj":"Protein"}],"text":"Multiplex cytokine assay\nQuantification of the levels of cytokines IL-2, IL-6, IL-10 and TNF and the chemokine CXCL8 was performed on culture supernatants using multiplexed biomarker immunoassay kits according to manufacturer's instructions (Bio-Rad Laboratories, Hercules, CA). A Bio-Plex™ 200 readout System was used (Bio-Rad), which utilizes Luminex® xMAP™ fluorescent bead-based technology (Luminex Corp., Austin). Levels were automatically calculated from standard curves using Bio-Plex Manager software (v.4.1.1, Bio-Rad)."}
bionlp-st-ge-2016-reference-tees
{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T13423","span":{"begin":57,"end":71},"obj":"Protein"},{"id":"T13424","span":{"begin":73,"end":77},"obj":"Protein"},{"id":"T13425","span":{"begin":79,"end":84},"obj":"Protein"},{"id":"T13426","span":{"begin":89,"end":92},"obj":"Protein"},{"id":"T13427","span":{"begin":111,"end":116},"obj":"Protein"},{"id":"T13428","span":{"begin":47,"end":53},"obj":"Gene_expression"},{"id":"T13429","span":{"begin":47,"end":53},"obj":"Gene_expression"},{"id":"T13430","span":{"begin":47,"end":53},"obj":"Gene_expression"},{"id":"T13431","span":{"begin":47,"end":53},"obj":"Gene_expression"},{"id":"T13432","span":{"begin":47,"end":53},"obj":"Gene_expression"},{"id":"T13433","span":{"begin":324,"end":327},"obj":"Protein"},{"id":"T13434","span":{"begin":523,"end":526},"obj":"Protein"}],"relations":[{"id":"R10813","pred":"themeOf","subj":"T13423","obj":"T13428"},{"id":"R10814","pred":"themeOf","subj":"T13424","obj":"T13429"},{"id":"R10815","pred":"themeOf","subj":"T13425","obj":"T13430"},{"id":"R10816","pred":"themeOf","subj":"T13426","obj":"T13431"},{"id":"R10817","pred":"themeOf","subj":"T13427","obj":"T13432"}],"text":"Multiplex cytokine assay\nQuantification of the levels of cytokines IL-2, IL-6, IL-10 and TNF and the chemokine CXCL8 was performed on culture supernatants using multiplexed biomarker immunoassay kits according to manufacturer's instructions (Bio-Rad Laboratories, Hercules, CA). A Bio-Plex™ 200 readout System was used (Bio-Rad), which utilizes Luminex® xMAP™ fluorescent bead-based technology (Luminex Corp., Austin). Levels were automatically calculated from standard curves using Bio-Plex Manager software (v.4.1.1, Bio-Rad)."}
bionlp-st-ge-2016-reference
{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T13204","span":{"begin":67,"end":71},"obj":"Protein"},{"id":"T13205","span":{"begin":73,"end":77},"obj":"Protein"},{"id":"T13206","span":{"begin":79,"end":84},"obj":"Protein"},{"id":"T13207","span":{"begin":111,"end":116},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Multiplex cytokine assay\nQuantification of the levels of cytokines IL-2, IL-6, IL-10 and TNF and the chemokine CXCL8 was performed on culture supernatants using multiplexed biomarker immunoassay kits according to manufacturer's instructions (Bio-Rad Laboratories, Hercules, CA). A Bio-Plex™ 200 readout System was used (Bio-Rad), which utilizes Luminex® xMAP™ fluorescent bead-based technology (Luminex Corp., Austin). Levels were automatically calculated from standard curves using Bio-Plex Manager software (v.4.1.1, Bio-Rad)."}
bionlp-st-ge-2016-uniprot
{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T13385","span":{"begin":67,"end":71},"obj":"P60568"},{"id":"T13386","span":{"begin":73,"end":77},"obj":"P05231"},{"id":"T13387","span":{"begin":79,"end":84},"obj":"P22301"},{"id":"T13388","span":{"begin":89,"end":92},"obj":"P01375"},{"id":"T13389","span":{"begin":111,"end":116},"obj":"P10145"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Multiplex cytokine assay\nQuantification of the levels of cytokines IL-2, IL-6, IL-10 and TNF and the chemokine CXCL8 was performed on culture supernatants using multiplexed biomarker immunoassay kits according to manufacturer's instructions (Bio-Rad Laboratories, Hercules, CA). A Bio-Plex™ 200 readout System was used (Bio-Rad), which utilizes Luminex® xMAP™ fluorescent bead-based technology (Luminex Corp., Austin). Levels were automatically calculated from standard curves using Bio-Plex Manager software (v.4.1.1, Bio-Rad)."}
test2
{"project":"test2","denotations":[{"id":"T13200","span":{"begin":67,"end":71},"obj":"Protein"},{"id":"T13201","span":{"begin":73,"end":77},"obj":"Protein"},{"id":"T13202","span":{"begin":79,"end":84},"obj":"Protein"},{"id":"T13203","span":{"begin":111,"end":116},"obj":"Protein"}],"text":"Multiplex cytokine assay\nQuantification of the levels of cytokines IL-2, IL-6, IL-10 and TNF and the chemokine CXCL8 was performed on culture supernatants using multiplexed biomarker immunoassay kits according to manufacturer's instructions (Bio-Rad Laboratories, Hercules, CA). A Bio-Plex™ 200 readout System was used (Bio-Rad), which utilizes Luminex® xMAP™ fluorescent bead-based technology (Luminex Corp., Austin). Levels were automatically calculated from standard curves using Bio-Plex Manager software (v.4.1.1, Bio-Rad)."}