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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T12823 0-4 NN denotes Cell
T12824 5-14 NN denotes culturing
T12825 14-15 -COMMA- denotes ,
T12826 16-28 NN denotes transfection
T12827 29-32 CC denotes and
T12828 33-44 NN denotes stimulation
T12829 45-51 NN denotes Jurkat
T12830 52-59 NN denotes T-cells
T12831 60-61 -LRB- denotes (
T12832 61-65 JJ denotes wild
T12833 66-70 NN denotes type
T12834 71-74 CC denotes and
T12835 75-78 NN denotes TCR
T12836 79-89 NN denotes deficient-
T12837 90-96 JJ denotes TCR-/-
T12838 96-97 -RRB- denotes )
T12839 98-102 VB denotes were
T12840 103-113 VB denotes maintained
T12841 114-116 IN denotes in
T12842 117-119 CD denotes 90
T12843 119-120 NN denotes %
T12844 121-125 NN denotes RPMI
T12845 126-130 CD denotes 1640
T12846 131-137 NN denotes medium
T12847 138-139 -LRB- denotes (
T12848 139-142 NN denotes PAA
T12849 143-155 NN denotes laboratories
T12850 155-156 -COMMA- denotes ,
T12851 157-164 NNP denotes Austria
T12852 164-165 -RRB- denotes )
T12853 166-170 IN denotes with
T12854 171-174 CD denotes 1.5
T12855 175-177 NN denotes mM
T12856 178-189 NN denotes L-glutamine
T12857 190-191 -LRB- denotes (
T12858 191-201 NN denotes Invitrogen
T12859 201-202 -COMMA- denotes ,
T12860 203-206 NNP denotes USA
T12861 206-207 -RRB- denotes )
T12862 207-208 -COMMA- denotes ,
T12863 209-211 CD denotes 10
T12864 211-212 NN denotes %
T12865 213-219 JJ denotes foetal
T12866 220-226 JJ denotes bovine
T12867 227-232 NN denotes serum
T12868 233-234 -LRB- denotes (
T12869 234-244 NN denotes Invitrogen
T12870 244-245 -COMMA- denotes ,
T12871 246-249 NNP denotes USA
T12872 249-250 -RRB- denotes )
T12873 251-254 CC denotes and
T12874 255-256 CD denotes 1
T12875 256-257 NN denotes %
T12876 258-280 JJ denotes antibiotic-antimycotic
T12877 281-282 -LRB- denotes (
T12878 282-292 NN denotes Invitrogen
T12879 292-293 -COMMA- denotes ,
T12880 294-297 NNP denotes USA
T12881 297-298 -RRB- denotes )
T12882 299-302 CC denotes and
T12883 303-312 VB denotes incubated
T12884 313-315 IN denotes in
T12885 316-317 DT denotes a
T12886 318-324 JJ denotes stable
T12887 325-336 NN denotes environment
T12888 337-339 IN denotes of
T12889 340-341 CD denotes 5
T12890 341-342 NN denotes %
T12891 343-346 NN denotes CO2
T12892 347-349 IN denotes at
T12893 350-354 NN denotes 37°C
T12894 356-359 DT denotes The
T12895 360-365 NN denotes cells
T12896 366-370 VB denotes were
T12897 371-382 VB denotes centrifuged
T12898 383-385 IN denotes at
T12899 386-390 CD denotes 1000
T12900 391-392 SYM denotes ×
T12901 393-394 NN denotes g
T12902 395-398 IN denotes for
T12903 399-400 CD denotes 8
T12904 401-404 NN denotes min
T12905 405-408 CC denotes and
T12906 409-420 VB denotes resuspended
T12907 421-423 IN denotes in
T12908 424-429 JJ denotes fresh
T12909 430-435 NN denotes media
T12910 436-438 TO denotes to
T12911 439-440 DT denotes a
T12912 441-446 JJ denotes final
T12913 447-451 NN denotes cell
T12914 452-459 NN denotes density
T12915 460-462 IN denotes of
T12916 463-466 CD denotes 1.6
T12917 467-468 CC denotes ×
T12918 469-472 CD denotes 107
T12919 473-481 NN denotes cells/ml
T12920 482-484 IN denotes in
T12921 485-486 DT denotes a
T12922 487-494 JJ denotes 24-well
T12923 495-500 NN denotes plate
T12924 502-510 NN denotes Reporter
T12925 511-518 NN denotes plasmid
T12926 519-520 -LRB- denotes (
T12927 520-529 NN denotes pNFκB-Luc
T12928 529-530 -COMMA- denotes ,
T12929 531-535 NN denotes pAP1
T12930 536-537 -LRB- denotes (
T12931 537-540 NN denotes PMA
T12932 540-541 -RRB- denotes )
T12933 541-548 NN denotes -TA-Luc
T12934 548-549 -COMMA- denotes ,
T12935 550-560 NN denotes pNFκB-SEAP
T12936 560-561 -RRB- denotes )
T12937 561-562 -COMMA- denotes ,
T12938 563-571 JJ denotes internal
T12939 572-579 NN denotes control
T12940 580-587 NN denotes plasmid
T12941 588-589 -LRB- denotes (
T12942 589-592 NN denotes pRL
T12943 592-593 -RRB- denotes )
T12944 594-595 -LRB- denotes (
T12945 595-602 NNP denotes Promega
T12946 602-603 -COMMA- denotes ,
T12947 604-607 NNP denotes USA
T12948 607-608 -RRB- denotes )
T12949 609-612 CC denotes and
T12950 613-626 NN denotes lipofectamine
T12951 627-631 CD denotes 2000
T12952 632-633 -LRB- denotes (
T12953 633-643 NN denotes Invitrogen
T12954 643-644 -COMMA- denotes ,
T12955 645-648 NNP denotes USA
T12956 648-649 -RRB- denotes )
T12957 650-654 VB denotes were
T12958 655-660 VB denotes added
T12959 661-663 TO denotes to
T12960 664-668 DT denotes each
T12961 669-673 RB denotes well
T12962 674-676 IN denotes at
T12963 677-681 CD denotes 0.54
T12964 682-689 RB denotes μg/well
T12965 689-690 -COMMA- denotes ,
T12966 691-695 CD denotes 0.06
T12967 696-703 JJ denotes μg/well
T12968 704-707 CC denotes and
T12969 708-711 CD denotes 1.5
T12970 712-719 NN denotes μl/well
T12971 719-720 -COMMA- denotes ,
T12972 721-733 RB denotes respectively
T12973 735-744 RB denotes Initially
T12974 744-745 -COMMA- denotes ,
T12975 746-749 DT denotes the
T12976 750-758 NN denotes reporter
T12977 759-766 NN denotes plasmid
T12978 767-770 CC denotes and
T12979 771-774 NN denotes pRL
T12980 775-779 VB denotes were
T12981 780-785 VB denotes mixed
T12982 786-796 RB denotes separately
T12983 797-801 IN denotes with
T12984 802-809 NN denotes OptiMEM
T12985 810-811 -LRB- denotes (
T12986 811-816 NNP denotes Gibco
T12987 816-817 -COMMA- denotes ,
T12988 818-821 NNP denotes USA
T12989 821-822 -RRB- denotes )
T12990 824-829 IN denotes After
T12991 830-831 CD denotes 5
T12992 832-835 NN denotes min
T12993 836-838 IN denotes of
T12994 839-849 NN denotes incubation
T12995 850-852 IN denotes at
T12996 853-857 NN denotes room
T12997 858-869 NN denotes temperature
T12998 869-870 -COMMA- denotes ,
T12999 871-884 NN denotes lipofectamine
T13000 885-889 CD denotes 2000
T13001 890-893 VB denotes was
T13002 894-899 VB denotes added
T13003 900-903 CC denotes and
T13004 904-907 DT denotes the
T13005 908-915 NN denotes mixture
T13006 916-919 VB denotes was
T13007 920-929 VB denotes incubated
T13008 930-937 RB denotes further
T13009 938-941 IN denotes for
T13010 942-944 CD denotes 20
T13011 945-948 NN denotes min
T13012 949-951 IN denotes at
T13013 952-956 NN denotes room
T13014 957-968 NN denotes temperature
T13015 970-973 DT denotes The
T13016 974-986 NN denotes transfection
T13017 987-990 VB denotes was
T13018 991-998 VB denotes allowed
T13019 999-1001 TO denotes to
T13020 1002-1009 VB denotes proceed
T13021 1010-1019 RB denotes overnight
T13022 1020-1022 IN denotes at
T13023 1023-1027 NN denotes 37°C
T13024 1027-1028 -COMMA- denotes ,
T13025 1029-1034 IN denotes after
T13026 1035-1040 WDT denotes which
T13027 1040-1041 -COMMA- denotes ,
T13028 1042-1045 DT denotes the
T13029 1046-1051 NN denotes cells
T13030 1052-1056 VB denotes were
T13031 1057-1068 VB denotes centrifuged
T13032 1068-1069 -COMMA- denotes ,
T13033 1070-1073 DT denotes the
T13034 1074-1079 NN denotes media
T13035 1080-1087 VB denotes removed
T13036 1088-1091 CC denotes and
T13037 1092-1097 JJ denotes fresh
T13038 1098-1108 JJ denotes pre-warmed
T13039 1109-1114 NN denotes media
T13040 1115-1120 VB denotes added
T13041 1122-1125 DT denotes The
T13042 1126-1131 NN denotes cells
T13043 1132-1136 VB denotes were
T13044 1137-1150 JJ denotes pre-incubated
T13045 1151-1155 IN denotes with
T13046 1156-1161 NN denotes NF-κB
T13047 1161-1162 -COMMA- denotes ,
T13048 1163-1166 NN denotes JNK
T13049 1167-1170 CC denotes and
T13050 1171-1174 NN denotes PKC
T13051 1175-1185 NN denotes inhibitors
T13052 1186-1189 CC denotes and
T13053 1190-1200 VB denotes stimulated
T13054 1201-1203 IN denotes in
T13055 1204-1211 JJ denotes 24-well
T13056 1212-1218 NN denotes plates
T13057 1219-1223 IN denotes with
T13058 1224-1233 JJ denotes different
T13059 1234-1248 NN denotes concentrations
T13060 1249-1251 IN denotes of
T13061 1252-1255 NN denotes PMA
T13062 1255-1256 -COMMA- denotes ,
T13063 1257-1259 NN denotes HK
T13064 1260-1262 FW denotes E.
T13065 1263-1267 FW denotes coli
T13066 1268-1274 NN denotes MG1655
T13067 1275-1278 CC denotes and
T13068 1279-1286 NNP denotes Calcium
T13069 1287-1296 NNP denotes Ionophore
T13070 1297-1303 NN denotes A23187
T13071 1305-1308 DT denotes The
T13072 1309-1314 NN denotes cells
T13073 1315-1319 VB denotes were
T13074 1320-1325 VB denotes lysed
T13075 1326-1329 CC denotes and
T13076 1330-1340 NN denotes luciferase
T13077 1341-1349 NN denotes activity
T13078 1350-1351 -LRB- denotes (
T13079 1351-1356 NN denotes NF-κB
T13080 1357-1360 CC denotes and
T13081 1361-1365 NN denotes AP-1
T13082 1365-1366 -RRB- denotes )
T13083 1367-1370 VB denotes was
T13084 1371-1379 VB denotes measured
T13085 1380-1385 VB denotes using
T13086 1386-1389 DT denotes the
T13087 1390-1406 JJ denotes Dual-Luciferase®
T13088 1407-1415 NN denotes reporter
T13089 1416-1421 NN denotes assay
T13090 1422-1428 NN denotes system
T13091 1429-1430 -LRB- denotes (
T13092 1430-1437 NNP denotes Promega
T13093 1437-1438 -COMMA- denotes ,
T13094 1439-1442 NNP denotes USA
T13095 1442-1443 -RRB- denotes )
T13096 1444-1453 VB denotes according
T13097 1454-1456 TO denotes to
T13098 1457-1460 DT denotes the
T13099 1461-1473 NN denotes manufacturer
T13100 1473-1475 POS denotes 's
T13101 1476-1488 NN denotes instructions
T13102 1489-1491 IN denotes on
T13103 1492-1493 DT denotes a
T13104 1494-1496 NN denotes TD
T13105 1497-1502 CD denotes 20/20
T13106 1503-1514 NN denotes luminometer
T13107 1515-1516 -LRB- denotes (
T13108 1516-1522 NNP denotes Turner
T13109 1523-1530 NNP denotes Designs
T13110 1530-1531 -COMMA- denotes ,
T13111 1532-1541 NNP denotes Sunnyvale
T13112 1541-1542 -COMMA- denotes ,
T13113 1543-1545 NNP denotes CA
T13114 1545-1546 -RRB- denotes )
T13115 1548-1556 VB denotes Secreted
T13116 1557-1565 NN denotes alkaline
T13117 1566-1577 NN denotes phosphatase
T13118 1578-1579 -LRB- denotes (
T13119 1579-1588 NN denotes NFκB-SEAP
T13120 1588-1589 -COMMA- denotes ,
T13121 1590-1596 NN denotes figure
T13122 1597-1599 NN denotes 4a
T13123 1599-1600 -COMMA- denotes ,
T13124 1601-1602 NN denotes b
T13125 1602-1603 -RRB- denotes )
T13126 1604-1610 NN denotes levels
T13127 1611-1615 VB denotes were
T13128 1616-1624 VB denotes measured
T13129 1625-1630 VB denotes using
T13130 1631-1636 NNP denotes Great
T13131 1637-1644 NNP denotes EscAPe™
T13132 1645-1649 NNP denotes SEAP
T13133 1650-1659 NNP denotes Detection
T13134 1660-1663 NN denotes Kit
T13135 1664-1665 -LRB- denotes (
T13136 1665-1673 NNP denotes Clontech
T13137 1673-1674 -COMMA- denotes ,
T13138 1675-1678 NNP denotes USA
T13139 1678-1679 -RRB- denotes )
R10311 T12824 T12823 arg1Of culturing,Cell
R10312 T12824 T12825 arg1Of culturing,","
R10313 T12825 T12827 arg1Of ",",and
R10314 T12826 T12825 arg2Of transfection,","
R10315 T12828 T12827 arg2Of stimulation,and
R10316 T12830 T12829 arg1Of T-cells,Jurkat
R10317 T12830 T12831 arg1Of T-cells,(
R10318 T12830 T12839 arg1Of T-cells,were
R10319 T12830 T12840 arg2Of T-cells,maintained
R10320 T12830 T12883 arg2Of T-cells,incubated
R10321 T12833 T12832 arg1Of type,wild
R10322 T12833 T12834 arg1Of type,and
R10323 T12834 T12831 arg2Of and,(
R10324 T12836 T12834 arg2Of deficient-,and
R10325 T12836 T12835 arg1Of deficient-,TCR
R10326 T12836 T12837 arg1Of deficient-,TCR-/-
R10327 T12838 T12831 arg3Of ),(
R10328 T12840 T12841 arg1Of maintained,in
R10329 T12840 T12882 arg1Of maintained,and
R10330 T12842 T12843 arg1Of 90,%
R10331 T12846 T12842 arg1Of medium,90
R10332 T12846 T12844 arg1Of medium,RPMI
R10333 T12846 T12845 arg1Of medium,1640
R10334 T12846 T12847 arg1Of medium,(
R10335 T12846 T12853 arg1Of medium,with
R10336 T12846 T12868 arg1Of medium,(
R10337 T12846 T12873 arg1Of medium,and
R10338 T12849 T12847 arg2Of laboratories,(
R10339 T12849 T12848 arg1Of laboratories,PAA
R10340 T12849 T12850 arg1Of laboratories,","
R10341 T12851 T12850 arg2Of Austria,","
R10342 T12852 T12847 arg3Of ),(
R10343 T12854 T12855 arg1Of 1.5,mM
R10344 T12856 T12854 arg1Of L-glutamine,1.5
R10345 T12856 T12857 arg1Of L-glutamine,(
R10346 T12856 T12862 arg1Of L-glutamine,","
R10347 T12858 T12857 arg2Of Invitrogen,(
R10348 T12858 T12859 arg1Of Invitrogen,","
R10349 T12858 T12860 arg1Of Invitrogen,USA
R10350 T12861 T12857 arg3Of ),(
R10351 T12862 T12853 arg2Of ",",with
R10352 T12864 T12863 arg1Of %,10
R10353 T12865 T12864 arg1Of foetal,%
R10354 T12867 T12862 arg2Of serum,","
R10355 T12867 T12865 arg1Of serum,foetal
R10356 T12867 T12866 arg1Of serum,bovine
R10357 T12869 T12868 arg2Of Invitrogen,(
R10358 T12869 T12870 arg1Of Invitrogen,","
R10359 T12871 T12870 arg2Of USA,","
R10360 T12872 T12868 arg3Of ),(
R10361 T12873 T12841 arg2Of and,in
R10362 T12874 T12875 arg1Of 1,%
R10363 T12876 T12873 arg2Of antibiotic-antimycotic,and
R10364 T12876 T12874 arg1Of antibiotic-antimycotic,1
R10365 T12876 T12877 arg1Of antibiotic-antimycotic,(
R10366 T12878 T12877 arg2Of Invitrogen,(
R10367 T12878 T12879 arg1Of Invitrogen,","
R10368 T12880 T12879 arg2Of USA,","
R10369 T12881 T12877 arg3Of ),(
R10370 T12882 T12839 arg2Of and,were
R10371 T12883 T12882 arg2Of incubated,and
R10372 T12883 T12884 arg1Of incubated,in
R10373 T12887 T12884 arg2Of environment,in
R10374 T12887 T12885 arg1Of environment,a
R10375 T12887 T12886 arg1Of environment,stable
R10376 T12887 T12888 arg1Of environment,of
R10377 T12887 T12892 arg1Of environment,at
R10378 T12889 T12890 arg1Of 5,%
R10379 T12891 T12888 arg2Of CO2,of
R10380 T12891 T12889 arg1Of CO2,5
R10381 T12893 T12892 arg2Of 37°C,at
R10382 T12895 T12894 arg1Of cells,The
R10383 T12895 T12896 arg1Of cells,were
R10384 T12895 T12897 arg2Of cells,centrifuged
R10385 T12895 T12906 arg1Of cells,resuspended
R10386 T12897 T12896 arg2Of centrifuged,were
R10387 T12897 T12898 arg1Of centrifuged,at
R10388 T12897 T12905 arg1Of centrifuged,and
R10389 T12901 T12898 arg2Of g,at
R10390 T12901 T12899 arg1Of g,1000
R10391 T12901 T12900 arg1Of g,×
R10392 T12901 T12902 arg1Of g,for
R10393 T12904 T12902 arg2Of min,for
R10394 T12904 T12903 arg1Of min,8
R10395 T12906 T12905 arg2Of resuspended,and
R10396 T12906 T12907 arg1Of resuspended,in
R10397 T12906 T12910 arg1Of resuspended,to
R10398 T12909 T12907 arg2Of media,in
R10399 T12909 T12908 arg1Of media,fresh
R10400 T12914 T12910 arg2Of density,to
R10401 T12914 T12911 arg1Of density,a
R10402 T12914 T12912 arg1Of density,final
R10403 T12914 T12913 arg1Of density,cell
R10404 T12914 T12915 arg1Of density,of
R10405 T12916 T12917 arg1Of 1.6,×
R10406 T12918 T12917 arg2Of 107,×
R10407 T12919 T12915 arg2Of cells/ml,of
R10408 T12919 T12916 arg1Of cells/ml,1.6
R10409 T12919 T12918 arg1Of cells/ml,107
R10410 T12919 T12920 arg1Of cells/ml,in
R10411 T12923 T12920 arg2Of plate,in
R10412 T12923 T12921 arg1Of plate,a
R10413 T12923 T12922 arg1Of plate,24-well
R10414 T12925 T12924 arg1Of plasmid,Reporter
R10415 T12925 T12926 arg1Of plasmid,(
R10416 T12925 T12937 arg1Of plasmid,","
R10417 T12927 T12926 arg2Of pNFκB-Luc,(
R10418 T12927 T12928 arg1Of pNFκB-Luc,","
R10419 T12927 T12934 arg1Of pNFκB-Luc,","
R10420 T12931 T12930 arg2Of PMA,(
R10421 T12932 T12930 arg3Of ),(
R10422 T12933 T12928 arg2Of -TA-Luc,","
R10459 T12966 T12968 arg1Of 0.06,and
R10423 T12933 T12929 arg1Of -TA-Luc,pAP1
R10424 T12933 T12930 arg1Of -TA-Luc,(
R10425 T12935 T12934 arg2Of pNFκB-SEAP,","
R10426 T12936 T12926 arg3Of ),(
R10427 T12937 T12944 arg1Of ",",(
R10428 T12937 T12949 arg1Of ",",and
R10429 T12940 T12937 arg2Of plasmid,","
R10430 T12940 T12938 arg1Of plasmid,internal
R10431 T12940 T12939 arg1Of plasmid,control
R10432 T12940 T12941 arg1Of plasmid,(
R10433 T12942 T12941 arg2Of pRL,(
R10434 T12943 T12941 arg3Of ),(
R10435 T12945 T12944 arg2Of Promega,(
R10436 T12945 T12946 arg1Of Promega,","
R10437 T12945 T12947 arg1Of Promega,USA
R10438 T12948 T12944 arg3Of ),(
R10439 T12949 T12957 arg1Of and,were
R10440 T12949 T12958 arg2Of and,added
R10441 T12950 T12949 arg2Of lipofectamine,and
R10442 T12950 T12951 arg1Of lipofectamine,2000
R10443 T12950 T12952 arg1Of lipofectamine,(
R10444 T12953 T12952 arg2Of Invitrogen,(
R10445 T12953 T12954 arg1Of Invitrogen,","
R10446 T12955 T12954 arg2Of USA,","
R10447 T12956 T12952 arg3Of ),(
R10448 T12958 T12957 arg2Of added,were
R10449 T12958 T12959 arg1Of added,to
R10450 T12958 T12962 arg1Of added,at
R10451 T12958 T12971 arg1Of added,","
R10452 T12958 T12972 arg1Of added,respectively
R10453 T12960 T12959 arg2Of each,to
R10454 T12962 T12961 arg1Of at,well
R10455 T12963 T12964 arg1Of 0.54,μg/well
R10456 T12963 T12965 arg1Of 0.54,","
R10457 T12965 T12962 arg2Of ",",at
R10458 T12966 T12967 arg1Of 0.06,μg/well
R10460 T12969 T12968 arg2Of 1.5,and
R10461 T12970 T12965 arg2Of μl/well,","
R10462 T12970 T12966 arg1Of μl/well,0.06
R10463 T12970 T12969 arg1Of μl/well,1.5
R10464 T12977 T12976 arg1Of plasmid,reporter
R10465 T12977 T12978 arg1Of plasmid,and
R10466 T12978 T12975 arg1Of and,the
R10467 T12978 T12980 arg1Of and,were
R10468 T12978 T12981 arg2Of and,mixed
R10469 T12979 T12978 arg2Of pRL,and
R10470 T12981 T12973 arg1Of mixed,Initially
R10471 T12981 T12974 arg1Of mixed,","
R10472 T12981 T12980 arg2Of mixed,were
R10473 T12981 T12982 arg1Of mixed,separately
R10474 T12981 T12983 arg1Of mixed,with
R10475 T12981 T12985 arg1Of mixed,(
R10476 T12984 T12983 arg2Of OptiMEM,with
R10477 T12986 T12985 arg2Of Gibco,(
R10478 T12986 T12987 arg1Of Gibco,","
R10479 T12986 T12988 arg1Of Gibco,USA
R10480 T12989 T12985 arg3Of ),(
R10481 T12992 T12990 arg2Of min,After
R10482 T12992 T12991 arg1Of min,5
R10483 T12992 T12993 arg1Of min,of
R10484 T12992 T12995 arg1Of min,at
R10485 T12994 T12993 arg2Of incubation,of
R10486 T12997 T12995 arg2Of temperature,at
R10487 T12997 T12996 arg1Of temperature,room
R10488 T12999 T13000 arg1Of lipofectamine,2000
R10489 T12999 T13001 arg1Of lipofectamine,was
R10490 T12999 T13002 arg2Of lipofectamine,added
R10491 T13002 T13001 arg2Of added,was
R10492 T13002 T13003 arg1Of added,and
R10493 T13003 T12990 arg1Of and,After
R10494 T13003 T12998 arg1Of and,","
R10495 T13005 T13004 arg1Of mixture,the
R10496 T13005 T13006 arg1Of mixture,was
R10497 T13005 T13007 arg2Of mixture,incubated
R10498 T13007 T13003 arg2Of incubated,and
R10499 T13007 T13006 arg2Of incubated,was
R10500 T13007 T13008 arg1Of incubated,further
R10501 T13007 T13009 arg1Of incubated,for
R10502 T13007 T13012 arg1Of incubated,at
R10503 T13011 T13009 arg2Of min,for
R10504 T13011 T13010 arg1Of min,20
R10541 T13046 T13047 arg1Of NF-κB,","
R10505 T13014 T13012 arg2Of temperature,at
R10506 T13014 T13013 arg1Of temperature,room
R10507 T13016 T13015 arg1Of transfection,The
R10508 T13016 T13017 arg1Of transfection,was
R10509 T13016 T13018 arg2Of transfection,allowed
R10510 T13016 T13020 arg1Of transfection,proceed
R10511 T13018 T13017 arg2Of allowed,was
R10512 T13018 T13040 arg2Of allowed,added
R10513 T13020 T13018 arg3Of proceed,allowed
R10514 T13020 T13019 arg1Of proceed,to
R10515 T13020 T13021 arg1Of proceed,overnight
R10516 T13020 T13022 arg1Of proceed,at
R10517 T13023 T13022 arg2Of 37°C,at
R10518 T13023 T13024 arg1Of 37°C,","
R10519 T13023 T13025 arg2Of 37°C,after
R10520 T13023 T13026 arg1Of 37°C,which
R10521 T13029 T13028 arg1Of cells,the
R10522 T13029 T13030 arg1Of cells,were
R10523 T13029 T13031 arg2Of cells,centrifuged
R10524 T13031 T13025 arg1Of centrifuged,after
R10525 T13031 T13027 arg1Of centrifuged,","
R10526 T13031 T13030 arg2Of centrifuged,were
R10527 T13034 T13035 arg2Of media,removed
R10528 T13034 T13036 arg1Of media,and
R10529 T13036 T13033 arg1Of and,the
R10530 T13036 T13040 arg1Of and,added
R10531 T13039 T13036 arg2Of media,and
R10532 T13039 T13037 arg1Of media,fresh
R10533 T13039 T13038 arg1Of media,pre-warmed
R10534 T13040 T13032 arg1Of added,","
R10535 T13042 T13041 arg1Of cells,The
R10536 T13042 T13043 arg1Of cells,were
R10537 T13042 T13044 arg1Of cells,pre-incubated
R10538 T13042 T13053 arg2Of cells,stimulated
R10539 T13044 T13045 arg1Of pre-incubated,with
R10540 T13044 T13052 arg1Of pre-incubated,and
R10542 T13047 T13049 arg1Of ",",and
R10543 T13048 T13047 arg2Of JNK,","
R10544 T13049 T13045 arg2Of and,with
R10545 T13051 T13049 arg2Of inhibitors,and
R10546 T13051 T13050 arg1Of inhibitors,PKC
R10547 T13052 T13043 arg2Of and,were
R10548 T13053 T13052 arg2Of stimulated,and
R10549 T13053 T13054 arg1Of stimulated,in
R10550 T13056 T13054 arg2Of plates,in
R10551 T13056 T13055 arg1Of plates,24-well
R10552 T13056 T13057 arg1Of plates,with
R10553 T13059 T13057 arg2Of concentrations,with
R10554 T13059 T13058 arg1Of concentrations,different
R10555 T13059 T13060 arg1Of concentrations,of
R10556 T13061 T13062 arg1Of PMA,","
R10557 T13062 T13067 arg1Of ",",and
R10558 T13066 T13062 arg2Of MG1655,","
R10559 T13066 T13063 arg1Of MG1655,HK
R10560 T13066 T13064 arg1Of MG1655,E.
R10561 T13066 T13065 arg1Of MG1655,coli
R10562 T13067 T13060 arg2Of and,of
R10563 T13069 T13068 arg1Of Ionophore,Calcium
R10564 T13070 T13067 arg2Of A23187,and
R10565 T13070 T13069 arg1Of A23187,Ionophore
R10566 T13072 T13071 arg1Of cells,The
R10567 T13072 T13073 arg1Of cells,were
R10568 T13072 T13074 arg2Of cells,lysed
R10569 T13074 T13073 arg2Of lysed,were
R10570 T13074 T13075 arg1Of lysed,and
R10571 T13077 T13076 arg1Of activity,luciferase
R10572 T13077 T13078 arg1Of activity,(
R10573 T13077 T13083 arg1Of activity,was
R10574 T13077 T13084 arg2Of activity,measured
R10575 T13079 T13080 arg1Of NF-κB,and
R10576 T13080 T13078 arg2Of and,(
R10577 T13081 T13080 arg2Of AP-1,and
R10578 T13082 T13078 arg3Of ),(
R10579 T13084 T13075 arg2Of measured,and
R10580 T13084 T13083 arg2Of measured,was
R10581 T13085 T13084 arg3Of using,measured
R10582 T13085 T13091 arg1Of using,(
R10583 T13085 T13096 arg1Of using,according
R10584 T13085 T13102 arg1Of using,on
R10585 T13090 T13085 arg2Of system,using
R10586 T13090 T13086 arg1Of system,the
R10623 T13126 T13128 arg2Of levels,measured
R10587 T13090 T13087 arg1Of system,Dual-Luciferase®
R10588 T13090 T13088 arg1Of system,reporter
R10589 T13090 T13089 arg1Of system,assay
R10590 T13092 T13091 arg2Of Promega,(
R10591 T13092 T13093 arg1Of Promega,","
R10592 T13092 T13094 arg1Of Promega,USA
R10593 T13095 T13091 arg3Of ),(
R10594 T13097 T13096 arg2Of to,according
R10595 T13099 T13098 arg1Of manufacturer,the
R10596 T13099 T13100 arg2Of manufacturer,'s
R10597 T13101 T13097 arg2Of instructions,to
R10598 T13101 T13100 arg1Of instructions,'s
R10599 T13106 T13102 arg2Of luminometer,on
R10600 T13106 T13103 arg1Of luminometer,a
R10601 T13106 T13104 arg1Of luminometer,TD
R10602 T13106 T13105 arg1Of luminometer,20/20
R10603 T13106 T13107 arg1Of luminometer,(
R10604 T13109 T13107 arg2Of Designs,(
R10605 T13109 T13108 arg1Of Designs,Turner
R10606 T13109 T13110 arg1Of Designs,","
R10607 T13111 T13110 arg2Of Sunnyvale,","
R10608 T13111 T13112 arg1Of Sunnyvale,","
R10609 T13113 T13112 arg2Of CA,","
R10610 T13114 T13107 arg3Of ),(
R10611 T13117 T13115 arg2Of phosphatase,Secreted
R10612 T13117 T13116 arg1Of phosphatase,alkaline
R10613 T13117 T13118 arg1Of phosphatase,(
R10614 T13117 T13127 modOf phosphatase,were
R10615 T13119 T13118 arg2Of NFκB-SEAP,(
R10616 T13119 T13120 arg1Of NFκB-SEAP,","
R10617 T13119 T13123 arg1Of NFκB-SEAP,","
R10618 T13122 T13120 arg2Of 4a,","
R10619 T13122 T13121 arg1Of 4a,figure
R10620 T13124 T13123 arg2Of b,","
R10621 T13125 T13118 arg3Of ),(
R10622 T13126 T13127 arg1Of levels,were
R10624 T13128 T13127 arg2Of measured,were
R10625 T13129 T13128 arg3Of using,measured
R10626 T13129 T13135 arg1Of using,(
R10627 T13133 T13130 arg1Of Detection,Great
R10628 T13133 T13131 arg1Of Detection,EscAPe™
R10629 T13133 T13132 arg1Of Detection,SEAP
R10630 T13134 T13129 arg2Of Kit,using
R10631 T13134 T13133 arg1Of Kit,Detection
R10632 T13136 T13135 arg2Of Clontech,(
R10633 T13136 T13137 arg1Of Clontech,","
R10634 T13136 T13138 arg1Of Clontech,USA
R10635 T13139 T13135 arg3Of ),(

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T12480 0-4 NN denotes Cell
T12481 5-14 NN denotes culturing
T12482 14-15 , denotes ,
T12483 16-28 NN denotes transfection
T12484 29-32 CC denotes and
T12485 33-44 NN denotes stimulation
T12486 45-51 NNP denotes Jurkat
T12487 52-59 NNS denotes T-cells
T12488 60-61 -LRB- denotes (
T12489 61-65 JJ denotes wild
T12490 66-70 NN denotes type
T12491 71-74 CC denotes and
T12492 75-78 NNP denotes TCR
T12493 79-88 NN denotes deficient
T12494 88-89 : denotes -
T12495 90-93 SYM denotes TCR
T12496 93-94 : denotes -
T12497 94-95 SYM denotes /
T12498 95-96 : denotes -
T12499 96-97 -RRB- denotes )
T12500 98-102 VBD denotes were
T12501 103-113 VBN denotes maintained
T12502 114-116 IN denotes in
T12503 117-119 CD denotes 90
T12504 119-120 NN denotes %
T12505 121-125 NNP denotes RPMI
T12506 126-130 CD denotes 1640
T12507 131-137 NN denotes medium
T12508 138-139 -LRB- denotes (
T12509 139-142 NNP denotes PAA
T12510 143-155 NNS denotes laboratories
T12511 155-156 , denotes ,
T12512 157-164 NNP denotes Austria
T12513 164-165 -RRB- denotes )
T12514 166-170 IN denotes with
T12515 171-174 CD denotes 1.5
T12516 175-177 NNP denotes mM
T12517 178-189 NNP denotes L-glutamine
T12518 190-191 -LRB- denotes (
T12519 191-201 NNP denotes Invitrogen
T12520 201-202 , denotes ,
T12521 203-206 NNP denotes USA
T12522 206-207 -RRB- denotes )
T12523 207-208 , denotes ,
T12524 209-211 CD denotes 10
T12525 211-212 NN denotes %
T12526 213-219 JJ denotes foetal
T12527 220-226 JJ denotes bovine
T12528 227-232 NN denotes serum
T12529 233-234 -LRB- denotes (
T12530 234-244 NNP denotes Invitrogen
T12531 244-245 , denotes ,
T12532 246-249 NNP denotes USA
T12533 249-250 -RRB- denotes )
T12534 251-254 CC denotes and
T12535 255-256 CD denotes 1
T12536 256-257 NN denotes %
T12537 258-280 JJ denotes antibiotic-antimycotic
T12538 281-282 -LRB- denotes (
T12539 282-292 NNP denotes Invitrogen
T12540 292-293 , denotes ,
T12541 294-297 NNP denotes USA
T12542 297-298 -RRB- denotes )
T12543 299-302 CC denotes and
T12544 303-312 VBN denotes incubated
T12545 313-315 IN denotes in
T12546 316-317 DT denotes a
T12547 318-324 JJ denotes stable
T12548 325-336 NN denotes environment
T12549 337-339 IN denotes of
T12550 340-341 CD denotes 5
T12551 341-342 NN denotes %
T12552 343-346 CD denotes CO2
T12553 347-349 IN denotes at
T12554 350-352 CD denotes 37
T12555 352-353 CD denotes °
T12556 353-354 NNP denotes C
T12557 354-355 . denotes .
T12558 356-359 DT denotes The
T12559 360-365 NNS denotes cells
T12560 366-370 VBD denotes were
T12561 371-382 VBN denotes centrifuged
T12562 383-385 IN denotes at
T12563 386-390 CD denotes 1000
T12564 391-392 CD denotes ×
T12565 393-394 NN denotes g
T12566 395-398 IN denotes for
T12567 399-400 CD denotes 8
T12568 401-404 NN denotes min
T12569 405-408 CC denotes and
T12570 409-420 VBD denotes resuspended
T12571 421-423 IN denotes in
T12572 424-429 JJ denotes fresh
T12573 430-435 NNS denotes media
T12574 436-438 TO denotes to
T12575 439-440 DT denotes a
T12576 441-446 JJ denotes final
T12577 447-451 NN denotes cell
T12578 452-459 NN denotes density
T12579 460-462 IN denotes of
T12580 463-466 CD denotes 1.6
T12581 467-468 CD denotes ×
T12582 469-472 CD denotes 107
T12583 473-481 NN denotes cells/ml
T12584 482-484 IN denotes in
T12585 485-486 DT denotes a
T12586 487-494 JJ denotes 24-well
T12587 495-500 NN denotes plate
T12588 500-501 . denotes .
T12589 502-510 NN denotes Reporter
T12590 511-518 NN denotes plasmid
T12591 519-520 -LRB- denotes (
T12592 520-529 JJ denotes pNFκB-Luc
T12593 529-530 , denotes ,
T12594 531-535 JJ denotes pAP1
T12595 536-537 -LRB- denotes (
T12596 537-540 NNP denotes PMA
T12597 540-541 -RRB- denotes )
T12598 541-542 : denotes -
T12599 542-548 NNP denotes TA-Luc
T12600 548-549 , denotes ,
T12601 550-560 NNP denotes pNFκB-SEAP
T12602 560-561 -RRB- denotes )
T12603 561-562 , denotes ,
T12604 563-571 JJ denotes internal
T12605 572-579 NN denotes control
T12606 580-587 NN denotes plasmid
T12607 588-589 -LRB- denotes (
T12608 589-592 NNP denotes pRL
T12609 592-593 -RRB- denotes )
T12610 594-595 -LRB- denotes (
T12611 595-602 NNP denotes Promega
T12612 602-603 , denotes ,
T12613 604-607 NNP denotes USA
T12614 607-608 -RRB- denotes )
T12615 609-612 CC denotes and
T12616 613-626 JJ denotes lipofectamine
T12617 627-631 CD denotes 2000
T12618 632-633 -LRB- denotes (
T12619 633-643 NNP denotes Invitrogen
T12620 643-644 , denotes ,
T12621 645-648 NNP denotes USA
T12622 648-649 -RRB- denotes )
T12623 650-654 VBD denotes were
T12624 655-660 VBN denotes added
T12625 661-663 TO denotes to
T12626 664-668 DT denotes each
T12627 669-673 RB denotes well
T12628 674-676 IN denotes at
T12629 677-681 CD denotes 0.54
T12630 682-684 NN denotes μg
T12631 684-685 RB denotes /
T12632 685-689 RB denotes well
T12633 689-690 , denotes ,
T12634 691-695 CD denotes 0.06
T12635 696-698 NN denotes μg
T12636 698-699 RB denotes /
T12637 699-703 RB denotes well
T12638 704-707 CC denotes and
T12639 708-711 CD denotes 1.5
T12640 712-714 NN denotes μl
T12641 714-715 RB denotes /
T12642 715-719 RB denotes well
T12643 719-720 , denotes ,
T12644 721-733 RB denotes respectively
T12645 733-734 . denotes .
T12646 735-744 RB denotes Initially
T12647 744-745 , denotes ,
T12648 746-749 DT denotes the
T12649 750-758 NN denotes reporter
T12650 759-766 NN denotes plasmid
T12651 767-770 CC denotes and
T12652 771-774 NN denotes pRL
T12653 775-779 VBD denotes were
T12654 780-785 JJ denotes mixed
T12655 786-796 RB denotes separately
T12656 797-801 IN denotes with
T12657 802-809 NNP denotes OptiMEM
T12658 810-811 -LRB- denotes (
T12659 811-816 NNP denotes Gibco
T12660 816-817 , denotes ,
T12661 818-821 NNP denotes USA
T12662 821-822 -RRB- denotes )
T12663 822-823 . denotes .
T12664 824-829 IN denotes After
T12665 830-831 CD denotes 5
T12666 832-835 NN denotes min
T12667 836-838 IN denotes of
T12668 839-849 NN denotes incubation
T12669 850-852 IN denotes at
T12670 853-857 NN denotes room
T12671 858-869 NN denotes temperature
T12672 869-870 , denotes ,
T12673 871-884 NN denotes lipofectamine
T12674 885-889 CD denotes 2000
T12675 890-893 VBD denotes was
T12676 894-899 VBN denotes added
T12677 900-903 CC denotes and
T12678 904-907 DT denotes the
T12679 908-915 NN denotes mixture
T12680 916-919 VBD denotes was
T12681 920-929 VBN denotes incubated
T12682 930-937 RBR denotes further
T12683 938-941 IN denotes for
T12684 942-944 CD denotes 20
T12685 945-948 NN denotes min
T12686 949-951 IN denotes at
T12687 952-956 NN denotes room
T12688 957-968 NN denotes temperature
T12689 968-969 . denotes .
T12690 970-973 DT denotes The
T12691 974-986 NN denotes transfection
T12692 987-990 VBD denotes was
T12693 991-998 VBN denotes allowed
T12694 999-1001 TO denotes to
T12695 1002-1009 VB denotes proceed
T12696 1010-1019 JJ denotes overnight
T12697 1020-1022 IN denotes at
T12698 1023-1025 CD denotes 37
T12699 1025-1026 CD denotes °
T12700 1026-1027 NNP denotes C
T12701 1027-1028 , denotes ,
T12702 1029-1034 IN denotes after
T12703 1035-1040 WDT denotes which
T12704 1040-1041 , denotes ,
T12705 1042-1045 DT denotes the
T12706 1046-1051 NNS denotes cells
T12707 1052-1056 VBD denotes were
T12708 1057-1068 VBN denotes centrifuged
T12709 1068-1069 , denotes ,
T12710 1070-1073 DT denotes the
T12711 1074-1079 NNS denotes media
T12712 1080-1087 VBD denotes removed
T12713 1088-1091 CC denotes and
T12714 1092-1097 JJ denotes fresh
T12715 1098-1108 JJ denotes pre-warmed
T12716 1109-1114 NNS denotes media
T12717 1115-1120 VBD denotes added
T12718 1120-1121 . denotes .
T12719 1122-1125 DT denotes The
T12720 1126-1131 NNS denotes cells
T12721 1132-1136 VBD denotes were
T12722 1137-1150 VBN denotes pre-incubated
T12723 1151-1155 IN denotes with
T12724 1156-1161 NNP denotes NF-κB
T12725 1161-1162 , denotes ,
T12726 1163-1166 NNP denotes JNK
T12727 1167-1170 CC denotes and
T12728 1171-1174 NNP denotes PKC
T12729 1175-1185 NNS denotes inhibitors
T12730 1186-1189 CC denotes and
T12731 1190-1200 VBN denotes stimulated
T12732 1201-1203 IN denotes in
T12733 1204-1211 JJ denotes 24-well
T12734 1212-1218 NNS denotes plates
T12735 1219-1223 IN denotes with
T12736 1224-1233 JJ denotes different
T12737 1234-1248 NNS denotes concentrations
T12738 1249-1251 IN denotes of
T12739 1252-1255 NNP denotes PMA
T12740 1255-1256 , denotes ,
T12741 1257-1259 NNP denotes HK
T12742 1260-1262 NNP denotes E.
T12743 1263-1267 NNS denotes coli
T12744 1268-1274 NNP denotes MG1655
T12745 1275-1278 CC denotes and
T12746 1279-1286 NNP denotes Calcium
T12747 1287-1296 NNP denotes Ionophore
T12748 1297-1303 NNP denotes A23187
T12749 1303-1304 . denotes .
T12750 1305-1308 DT denotes The
T12751 1309-1314 NNS denotes cells
T12752 1315-1319 VBD denotes were
T12753 1320-1325 VBN denotes lysed
T12754 1326-1329 CC denotes and
T12755 1330-1340 JJ denotes luciferase
T12756 1341-1349 NN denotes activity
T12757 1350-1351 -LRB- denotes (
T12758 1351-1356 NN denotes NF-κB
T12759 1357-1360 CC denotes and
T12760 1361-1365 NN denotes AP-1
T12761 1365-1366 -RRB- denotes )
T12762 1367-1370 VBD denotes was
T12763 1371-1379 VBN denotes measured
T12764 1380-1385 VBG denotes using
T12765 1386-1389 DT denotes the
T12766 1390-1405 NNP denotes Dual-Luciferase
T12767 1405-1406 NN denotes ®
T12768 1407-1415 NN denotes reporter
T12769 1416-1421 NN denotes assay
T12770 1422-1428 NN denotes system
T12771 1429-1430 -LRB- denotes (
T12772 1430-1437 NNP denotes Promega
T12773 1437-1438 , denotes ,
T12774 1439-1442 NNP denotes USA
T12775 1442-1443 -RRB- denotes )
T12776 1444-1453 VBG denotes according
T12777 1454-1456 TO denotes to
T12778 1457-1460 DT denotes the
T12779 1461-1473 NN denotes manufacturer
T12780 1473-1475 POS denotes 's
T12781 1476-1488 NNS denotes instructions
T12782 1489-1491 IN denotes on
T12783 1492-1493 DT denotes a
T12784 1494-1496 NNP denotes TD
T12785 1497-1502 CD denotes 20/20
T12786 1503-1514 NN denotes luminometer
T12787 1515-1516 -LRB- denotes (
T12788 1516-1522 NNP denotes Turner
T12789 1523-1530 NNS denotes Designs
T12790 1530-1531 , denotes ,
T12791 1532-1541 NNP denotes Sunnyvale
T12792 1541-1542 , denotes ,
T12793 1543-1545 NNP denotes CA
T12794 1545-1546 -RRB- denotes )
T12795 1546-1547 . denotes .
T12796 1548-1556 JJ denotes Secreted
T12797 1557-1565 NN denotes alkaline
T12798 1566-1577 NN denotes phosphatase
T12799 1578-1579 -LRB- denotes (
T12800 1579-1588 NNP denotes NFκB-SEAP
T12801 1588-1589 , denotes ,
T12802 1590-1596 NN denotes figure
T12803 1597-1599 NN denotes 4a
T12804 1599-1600 , denotes ,
T12805 1601-1602 NN denotes b
T12806 1602-1603 -RRB- denotes )
T12807 1604-1610 NNS denotes levels
T12808 1611-1615 VBD denotes were
T12809 1616-1624 VBN denotes measured
T12810 1625-1630 VBG denotes using
T12811 1631-1636 NNP denotes Great
T12812 1637-1643 NNP denotes EscAPe
T12813 1643-1644 VBD denotes ™
T12814 1645-1649 NNP denotes SEAP
T12815 1650-1659 NNP denotes Detection
T12816 1660-1663 NNP denotes Kit
T12817 1664-1665 -LRB- denotes (
T12818 1665-1673 NNP denotes Clontech
T12819 1673-1674 , denotes ,
T12820 1675-1678 NNP denotes USA
T12821 1678-1679 -RRB- denotes )
T12822 1679-1680 . denotes .
R9968 T12480 T12481 compound Cell,culturing
R9969 T12481 T12481 ROOT culturing,culturing
R9970 T12482 T12481 punct ",",culturing
R9971 T12483 T12487 nmod transfection,T-cells
R9972 T12484 T12483 cc and,transfection
R9973 T12485 T12483 conj stimulation,transfection
R9974 T12486 T12487 compound Jurkat,T-cells
R9975 T12487 T12481 appos T-cells,culturing
R9976 T12488 T12487 punct (,T-cells
R9977 T12489 T12490 amod wild,type
R9978 T12490 T12487 appos type,T-cells
R9979 T12491 T12490 cc and,type
R9980 T12492 T12501 nsubj TCR,maintained
R9981 T12493 T12501 prep deficient,maintained
R9982 T12494 T12495 punct -,TCR
R9983 T12495 T12501 punct TCR,maintained
R9984 T12496 T12501 punct -,maintained
R9985 T12497 T12501 punct /,maintained
R9986 T12498 T12501 punct -,maintained
R9987 T12499 T12501 punct ),maintained
R9988 T12500 T12501 auxpass were,maintained
R9989 T12501 T12487 parataxis maintained,T-cells
R9990 T12502 T12501 prep in,maintained
R9991 T12503 T12504 nummod 90,%
R9992 T12504 T12507 compound %,medium
R9993 T12505 T12507 nmod RPMI,medium
R9994 T12506 T12507 compound 1640,medium
R9995 T12507 T12502 pobj medium,in
R9996 T12508 T12510 punct (,laboratories
R9997 T12509 T12510 compound PAA,laboratories
R9998 T12510 T12507 appos laboratories,medium
R9999 T12511 T12510 punct ",",laboratories
R10000 T12512 T12510 appos Austria,laboratories
R10001 T12513 T12510 punct ),laboratories
R10002 T12514 T12510 prep with,laboratories
R10003 T12515 T12517 nummod 1.5,L-glutamine
R10004 T12516 T12517 compound mM,L-glutamine
R10005 T12517 T12514 pobj L-glutamine,with
R10006 T12518 T12519 punct (,Invitrogen
R10007 T12519 T12517 appos Invitrogen,L-glutamine
R10008 T12520 T12519 punct ",",Invitrogen
R10009 T12521 T12519 npadvmod USA,Invitrogen
R10010 T12522 T12519 punct ),Invitrogen
R10011 T12523 T12517 punct ",",L-glutamine
R10012 T12524 T12525 nummod 10,%
R10013 T12525 T12528 nmod %,serum
R10014 T12526 T12528 amod foetal,serum
R10015 T12527 T12528 amod bovine,serum
R10016 T12528 T12517 appos serum,L-glutamine
R10017 T12529 T12530 punct (,Invitrogen
R10018 T12530 T12528 appos Invitrogen,serum
R10019 T12531 T12530 punct ",",Invitrogen
R10020 T12532 T12530 appos USA,Invitrogen
R10021 T12533 T12530 punct ),Invitrogen
R10025 T12537 T12528 conj antibiotic-antimycotic,serum
R10026 T12538 T12541 punct (,USA
R10027 T12539 T12541 dep Invitrogen,USA
R10028 T12540 T12541 punct ",",USA
R10029 T12541 T12537 appos USA,antibiotic-antimycotic
R10030 T12542 T12541 punct ),USA
R10031 T12543 T12537 cc and,antibiotic-antimycotic
R10032 T12544 T12537 conj incubated,antibiotic-antimycotic
R10033 T12545 T12544 prep in,incubated
R10034 T12546 T12548 det a,environment
R10035 T12547 T12548 amod stable,environment
R10036 T12548 T12545 pobj environment,in
R10037 T12549 T12548 prep of,environment
R10038 T12550 T12551 nummod 5,%
R10039 T12551 T12549 pobj %,of
R10040 T12552 T12551 appos CO2,%
R10041 T12553 T12552 prep at,CO2
R10042 T12554 T12553 pobj 37,at
R10043 T12555 T12556 nummod °,C
R10044 T12556 T12552 appos C,CO2
R10045 T12557 T12501 punct .,maintained
R10046 T12558 T12559 det The,cells
R10047 T12559 T12561 nsubjpass cells,centrifuged
R10048 T12560 T12561 auxpass were,centrifuged
R10049 T12561 T12561 ROOT centrifuged,centrifuged
R10050 T12562 T12561 prep at,centrifuged
R10051 T12563 T12564 nummod 1000,×
R10052 T12564 T12562 pobj ×,at
R10053 T12565 T12561 conj g,centrifuged
R10054 T12566 T12565 prep for,g
R10055 T12567 T12568 nummod 8,min
R10056 T12568 T12566 pobj min,for
R10057 T12569 T12565 cc and,g
R10058 T12570 T12561 conj resuspended,centrifuged
R10059 T12571 T12570 prep in,resuspended
R10060 T12572 T12573 amod fresh,media
R10061 T12573 T12571 pobj media,in
R10062 T12574 T12570 prep to,resuspended
R10063 T12575 T12578 det a,density
R10064 T12576 T12578 amod final,density
R10065 T12577 T12578 compound cell,density
R10066 T12578 T12574 pobj density,to
R10067 T12579 T12578 prep of,density
R10068 T12580 T12583 nummod 1.6,cells/ml
R10069 T12581 T12583 nummod ×,cells/ml
R10070 T12582 T12583 nummod 107,cells/ml
R10071 T12583 T12579 pobj cells/ml,of
R10072 T12584 T12578 prep in,density
R10073 T12585 T12587 det a,plate
R10074 T12586 T12587 amod 24-well,plate
R10075 T12587 T12584 pobj plate,in
R10076 T12588 T12561 punct .,centrifuged
R10077 T12589 T12590 compound Reporter,plasmid
R10078 T12590 T12599 nmod plasmid,TA-Luc
R10079 T12591 T12596 punct (,PMA
R10080 T12592 T12596 nmod pNFκB-Luc,PMA
R10081 T12593 T12596 punct ",",PMA
R10082 T12594 T12596 nmod pAP1,PMA
R10083 T12595 T12596 punct (,PMA
R10084 T12596 T12590 appos PMA,plasmid
R10085 T12597 T12596 punct ),PMA
R10086 T12598 T12599 punct -,TA-Luc
R10087 T12599 T12599 ROOT TA-Luc,TA-Luc
R10088 T12600 T12599 punct ",",TA-Luc
R10089 T12601 T12599 conj pNFκB-SEAP,TA-Luc
R10090 T12602 T12599 punct ),TA-Luc
R10091 T12603 T12599 punct ",",TA-Luc
R10092 T12604 T12606 amod internal,plasmid
R10093 T12605 T12606 compound control,plasmid
R10094 T12606 T12599 appos plasmid,TA-Luc
R10095 T12607 T12608 punct (,pRL
R10096 T12608 T12606 appos pRL,plasmid
R10097 T12609 T12599 punct ),TA-Luc
R10098 T12610 T12611 punct (,Promega
R10099 T12611 T12599 appos Promega,TA-Luc
R10100 T12612 T12611 punct ",",Promega
R10101 T12613 T12611 appos USA,Promega
R10102 T12614 T12611 punct ),Promega
R10103 T12615 T12599 cc and,TA-Luc
R10104 T12616 T12624 nsubjpass lipofectamine,added
R10105 T12617 T12616 nummod 2000,lipofectamine
R10106 T12618 T12619 punct (,Invitrogen
R10107 T12619 T12616 appos Invitrogen,lipofectamine
R10108 T12620 T12619 punct ",",Invitrogen
R10109 T12621 T12619 appos USA,Invitrogen
R10110 T12622 T12619 punct ),Invitrogen
R10111 T12623 T12624 auxpass were,added
R10112 T12624 T12624 ROOT added,added
R10113 T12625 T12624 prep to,added
R10114 T12626 T12627 det each,well
R10115 T12627 T12625 pobj well,to
R10116 T12628 T12627 prep at,well
R10117 T12629 T12630 nummod 0.54,μg
R10118 T12630 T12628 pobj μg,at
R10119 T12631 T12632 advmod /,well
R10120 T12632 T12627 advmod well,well
R10121 T12633 T12644 punct ",",respectively
R10122 T12634 T12635 nummod 0.06,μg
R10123 T12635 T12644 npadvmod μg,respectively
R10124 T12636 T12637 advmod /,well
R10125 T12637 T12639 advmod well,1.5
R10126 T12638 T12637 cc and,well
R10127 T12639 T12640 nummod 1.5,μl
R10128 T12640 T12635 appos μl,μg
R10129 T12641 T12642 advmod /,well
R10130 T12642 T12644 intj well,respectively
R10131 T12643 T12644 punct ",",respectively
R10132 T12644 T12632 advmod respectively,well
R10133 T12645 T12624 punct .,added
R10134 T12646 T12653 advmod Initially,were
R10135 T12647 T12653 punct ",",were
R10136 T12648 T12650 det the,plasmid
R10137 T12649 T12650 compound reporter,plasmid
R10138 T12650 T12653 nsubj plasmid,were
R10139 T12651 T12650 cc and,plasmid
R10140 T12652 T12650 conj pRL,plasmid
R10141 T12653 T12653 ROOT were,were
R10142 T12654 T12653 acomp mixed,were
R10143 T12655 T12654 advmod separately,mixed
R10144 T12656 T12654 prep with,mixed
R10145 T12657 T12656 pobj OptiMEM,with
R10146 T12658 T12657 punct (,OptiMEM
R10147 T12659 T12657 appos Gibco,OptiMEM
R10148 T12660 T12659 punct ",",Gibco
R10149 T12661 T12659 appos USA,Gibco
R10150 T12662 T12659 punct ),Gibco
R10151 T12663 T12653 punct .,were
R10152 T12664 T12676 prep After,added
R10153 T12665 T12666 nummod 5,min
R10154 T12666 T12664 pobj min,After
R10155 T12667 T12666 prep of,min
R10156 T12668 T12667 pobj incubation,of
R10157 T12669 T12666 prep at,min
R10158 T12670 T12671 compound room,temperature
R10159 T12671 T12669 pobj temperature,at
R10160 T12672 T12676 punct ",",added
R10161 T12673 T12676 nsubjpass lipofectamine,added
R10162 T12674 T12673 nummod 2000,lipofectamine
R10163 T12675 T12676 auxpass was,added
R10164 T12676 T12676 ROOT added,added
R10165 T12677 T12676 cc and,added
R10166 T12678 T12679 det the,mixture
R10167 T12679 T12681 nsubjpass mixture,incubated
R10168 T12680 T12681 auxpass was,incubated
R10169 T12681 T12676 conj incubated,added
R10170 T12682 T12681 advmod further,incubated
R10171 T12683 T12681 prep for,incubated
R10172 T12684 T12685 nummod 20,min
R10177 T12689 T12681 punct .,incubated
R10178 T12690 T12691 det The,transfection
R10179 T12691 T12693 nsubjpass transfection,allowed
R10180 T12692 T12693 auxpass was,allowed
R10181 T12693 T12693 ROOT allowed,allowed
R10182 T12694 T12695 aux to,proceed
R10183 T12695 T12693 xcomp proceed,allowed
R10184 T12696 T12695 dobj overnight,proceed
R10185 T12697 T12695 prep at,proceed
R10186 T12698 T12700 nummod 37,C
R10187 T12699 T12700 nummod °,C
R10188 T12700 T12697 pobj C,at
R10189 T12701 T12695 punct ",",proceed
R10190 T12702 T12708 prep after,centrifuged
R10191 T12703 T12702 pobj which,after
R10192 T12704 T12708 punct ",",centrifuged
R10193 T12705 T12706 det the,cells
R10194 T12706 T12708 nsubjpass cells,centrifuged
R10195 T12707 T12708 auxpass were,centrifuged
R10196 T12708 T12712 advmod centrifuged,removed
R10197 T12709 T12712 punct ",",removed
R10198 T12710 T12711 det the,media
R10199 T12711 T12712 nsubj media,removed
R10200 T12712 T12693 advcl removed,allowed
R10201 T12713 T12712 cc and,removed
R10202 T12714 T12716 amod fresh,media
R10203 T12715 T12716 compound pre-warmed,media
R10204 T12716 T12717 nsubj media,added
R10205 T12717 T12712 conj added,removed
R10206 T12718 T12717 punct .,added
R10207 T12719 T12720 det The,cells
R10208 T12720 T12722 nsubjpass cells,pre-incubated
R10209 T12721 T12722 auxpass were,pre-incubated
R10210 T12722 T12722 ROOT pre-incubated,pre-incubated
R10211 T12723 T12722 prep with,pre-incubated
R10212 T12724 T12723 pobj NF-κB,with
R10213 T12725 T12724 punct ",",NF-κB
R10214 T12726 T12724 conj JNK,NF-κB
R10215 T12727 T12726 cc and,JNK
R10216 T12728 T12726 conj PKC,JNK
R10217 T12729 T12723 pobj inhibitors,with
R10218 T12730 T12722 cc and,pre-incubated
R10219 T12731 T12722 conj stimulated,pre-incubated
R10220 T12732 T12731 prep in,stimulated
R10221 T12733 T12734 amod 24-well,plates
R10222 T12734 T12732 pobj plates,in
R10223 T12735 T12731 prep with,stimulated
R10224 T12736 T12737 amod different,concentrations
R10225 T12737 T12735 pobj concentrations,with
R10226 T12738 T12737 prep of,concentrations
R10227 T12739 T12738 pobj PMA,of
R10228 T12740 T12739 punct ",",PMA
R10229 T12741 T12744 compound HK,MG1655
R10230 T12742 T12744 compound E.,MG1655
R10231 T12743 T12744 compound coli,MG1655
R10232 T12744 T12739 conj MG1655,PMA
R10233 T12745 T12744 cc and,MG1655
R10234 T12746 T12748 compound Calcium,A23187
R10235 T12747 T12748 compound Ionophore,A23187
R10236 T12748 T12744 conj A23187,MG1655
R10237 T12749 T12722 punct .,pre-incubated
R10238 T12750 T12751 det The,cells
R10239 T12751 T12753 nsubjpass cells,lysed
R10240 T12752 T12753 auxpass were,lysed
R10241 T12753 T12753 ROOT lysed,lysed
R10242 T12754 T12753 cc and,lysed
R10243 T12755 T12756 amod luciferase,activity
R10244 T12756 T12763 nsubjpass activity,measured
R10245 T12757 T12758 punct (,NF-κB
R10246 T12758 T12756 appos NF-κB,activity
R10247 T12759 T12758 cc and,NF-κB
R10248 T12760 T12758 conj AP-1,NF-κB
R10249 T12761 T12758 punct ),NF-κB
R10250 T12762 T12763 auxpass was,measured
R10251 T12763 T12753 conj measured,lysed
R10252 T12764 T12763 advcl using,measured
R10253 T12765 T12770 det the,system
R10254 T12766 T12770 nmod Dual-Luciferase,system
R10255 T12767 T12770 compound ®,system
R10256 T12768 T12770 compound reporter,system
R10257 T12769 T12770 compound assay,system
R10258 T12770 T12764 dobj system,using
R10259 T12771 T12770 punct (,system
R10260 T12772 T12770 appos Promega,system
R10261 T12773 T12772 punct ",",Promega
R10262 T12774 T12772 appos USA,Promega
R10263 T12775 T12770 punct ),system
R10264 T12776 T12763 prep according,measured
R10265 T12777 T12776 prep to,according
R10266 T12778 T12779 det the,manufacturer
R10267 T12779 T12781 poss manufacturer,instructions
R10268 T12780 T12779 case 's,manufacturer
R10269 T12781 T12777 pobj instructions,to
R10270 T12782 T12781 prep on,instructions
R10271 T12783 T12786 det a,luminometer
R10272 T12784 T12786 nmod TD,luminometer
R10273 T12785 T12786 nummod 20/20,luminometer
R10274 T12786 T12782 pobj luminometer,on
R10275 T12787 T12786 punct (,luminometer
R10276 T12788 T12789 compound Turner,Designs
R10277 T12789 T12786 appos Designs,luminometer
R10278 T12790 T12789 punct ",",Designs
R10279 T12791 T12789 conj Sunnyvale,Designs
R10280 T12792 T12791 punct ",",Sunnyvale
R10281 T12793 T12791 conj CA,Sunnyvale
R10282 T12794 T12791 punct ),Sunnyvale
R10283 T12795 T12763 punct .,measured
R10284 T12796 T12798 amod Secreted,phosphatase
R10285 T12797 T12798 amod alkaline,phosphatase
R10286 T12798 T12800 nmod phosphatase,NFκB-SEAP
R10287 T12799 T12800 punct (,NFκB-SEAP
R10288 T12800 T12807 nmod NFκB-SEAP,levels
R10289 T12801 T12800 punct ",",NFκB-SEAP
R10290 T12802 T12803 compound figure,4a
R10291 T12803 T12800 appos 4a,NFκB-SEAP
R10292 T12804 T12805 punct ",",b
R10293 T12805 T12800 appos b,NFκB-SEAP
R10294 T12806 T12805 punct ),b
R10295 T12807 T12809 nsubjpass levels,measured
R10296 T12808 T12809 auxpass were,measured
R10297 T12809 T12809 ROOT measured,measured
R10298 T12810 T12809 advcl using,measured
R10299 T12811 T12812 compound Great,EscAPe
R10300 T12812 T12816 compound EscAPe,Kit
R10301 T12813 T12816 compound ™,Kit
R10302 T12814 T12816 compound SEAP,Kit
R10303 T12815 T12816 compound Detection,Kit
R10304 T12816 T12810 dobj Kit,using
R10305 T12817 T12816 punct (,Kit
R10306 T12818 T12816 appos Clontech,Kit
R10307 T12819 T12818 punct ",",Clontech
R10308 T12820 T12818 npadvmod USA,Clontech
R10309 T12821 T12816 punct ),Kit
R10310 T12822 T12809 punct .,measured
R10022 T12534 T12528 cc and,serum
R10023 T12535 T12536 nummod 1,%
R10024 T12536 T12537 compound %,antibiotic-antimycotic
R10173 T12685 T12683 pobj min,for
R10174 T12686 T12685 prep at,min
R10175 T12687 T12688 compound room,temperature
R10176 T12688 T12686 pobj temperature,at

UBERON-AE

Id Subject Object Predicate Lexical cue
T12467 227-232 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

GO-BP

Id Subject Object Predicate Lexical cue
T13153 75-78 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T13154 90-93 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T13155 1163-1166 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T13156 1171-1174 http://purl.obolibrary.org/obo/GO_0004697 denotes PKC
T13157 1287-1296 http://purl.obolibrary.org/obo/GO_0022809 denotes Ionophore
T13158 1330-1349 http://purl.obolibrary.org/obo/GO_0045289 denotes luciferase activity
T13159 1330-1349 http://purl.obolibrary.org/obo/GO_0047077 denotes luciferase activity
T13160 1330-1349 http://purl.obolibrary.org/obo/GO_0047712 denotes luciferase activity
T13161 1330-1349 http://purl.obolibrary.org/obo/GO_0050248 denotes luciferase activity
T13162 1330-1349 http://purl.obolibrary.org/obo/GO_0050397 denotes luciferase activity
T13163 1543-1545 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T13164 1566-1577 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase

GO-MF

Id Subject Object Predicate Lexical cue
T13165 1163-1166 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T13166 1171-1174 http://purl.obolibrary.org/obo/GO_0004697 denotes PKC
T13167 1287-1296 http://purl.obolibrary.org/obo/GO_0022809 denotes Ionophore
T13168 1330-1349 http://purl.obolibrary.org/obo/GO_0045289 denotes luciferase activity
T13169 1330-1349 http://purl.obolibrary.org/obo/GO_0047077 denotes luciferase activity
T13170 1330-1349 http://purl.obolibrary.org/obo/GO_0047712 denotes luciferase activity
T13171 1330-1349 http://purl.obolibrary.org/obo/GO_0050248 denotes luciferase activity
T13172 1330-1349 http://purl.obolibrary.org/obo/GO_0050397 denotes luciferase activity
T13173 1543-1545 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T13174 1566-1577 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase

GO-CC

Id Subject Object Predicate Lexical cue
T13175 0-4 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T13176 54-59 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13177 360-365 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13178 473-478 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13179 1046-1051 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13180 1126-1131 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13181 1309-1314 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13182 75-78 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T13183 90-93 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T13184 139-142 http://purl.obolibrary.org/obo/GO_1990295 denotes PAA

sentences

Id Subject Object Predicate Lexical cue
T12470 0-44 Sentence denotes Cell culturing, transfection and stimulation
T12471 45-355 Sentence denotes Jurkat T-cells (wild type and TCR deficient- TCR-/-) were maintained in 90% RPMI 1640 medium (PAA laboratories, Austria) with 1.5 mM L-glutamine (Invitrogen, USA), 10% foetal bovine serum (Invitrogen, USA) and 1% antibiotic-antimycotic (Invitrogen, USA) and incubated in a stable environment of 5% CO2 at 37°C.
T12472 356-501 Sentence denotes The cells were centrifuged at 1000 × g for 8 min and resuspended in fresh media to a final cell density of 1.6 × 107 cells/ml in a 24-well plate.
T12473 502-734 Sentence denotes Reporter plasmid (pNFκB-Luc, pAP1 (PMA)-TA-Luc, pNFκB-SEAP), internal control plasmid (pRL) (Promega, USA) and lipofectamine 2000 (Invitrogen, USA) were added to each well at 0.54 μg/well, 0.06 μg/well and 1.5 μl/well, respectively.
T12474 735-823 Sentence denotes Initially, the reporter plasmid and pRL were mixed separately with OptiMEM (Gibco, USA).
T12475 824-969 Sentence denotes After 5 min of incubation at room temperature, lipofectamine 2000 was added and the mixture was incubated further for 20 min at room temperature.
T12476 970-1121 Sentence denotes The transfection was allowed to proceed overnight at 37°C, after which, the cells were centrifuged, the media removed and fresh pre-warmed media added.
T12477 1122-1304 Sentence denotes The cells were pre-incubated with NF-κB, JNK and PKC inhibitors and stimulated in 24-well plates with different concentrations of PMA, HK E. coli MG1655 and Calcium Ionophore A23187.
T12478 1305-1547 Sentence denotes The cells were lysed and luciferase activity (NF-κB and AP-1) was measured using the Dual-Luciferase® reporter assay system (Promega, USA) according to the manufacturer's instructions on a TD 20/20 luminometer (Turner Designs, Sunnyvale, CA).
T12479 1548-1680 Sentence denotes Secreted alkaline phosphatase (NFκB-SEAP, figure 4a, b) levels were measured using Great EscAPe™ SEAP Detection Kit (Clontech, USA).
T199 0-44 Sentence denotes Cell culturing, transfection and stimulation
T200 45-355 Sentence denotes Jurkat T-cells (wild type and TCR deficient- TCR-/-) were maintained in 90% RPMI 1640 medium (PAA laboratories, Austria) with 1.5 mM L-glutamine (Invitrogen, USA), 10% foetal bovine serum (Invitrogen, USA) and 1% antibiotic-antimycotic (Invitrogen, USA) and incubated in a stable environment of 5% CO2 at 37°C.
T201 356-501 Sentence denotes The cells were centrifuged at 1000 × g for 8 min and resuspended in fresh media to a final cell density of 1.6 × 107 cells/ml in a 24-well plate.
T202 502-734 Sentence denotes Reporter plasmid (pNFκB-Luc, pAP1 (PMA)-TA-Luc, pNFκB-SEAP), internal control plasmid (pRL) (Promega, USA) and lipofectamine 2000 (Invitrogen, USA) were added to each well at 0.54 μg/well, 0.06 μg/well and 1.5 μl/well, respectively.
T203 735-823 Sentence denotes Initially, the reporter plasmid and pRL were mixed separately with OptiMEM (Gibco, USA).
T204 824-969 Sentence denotes After 5 min of incubation at room temperature, lipofectamine 2000 was added and the mixture was incubated further for 20 min at room temperature.
T205 970-1121 Sentence denotes The transfection was allowed to proceed overnight at 37°C, after which, the cells were centrifuged, the media removed and fresh pre-warmed media added.
T206 1122-1304 Sentence denotes The cells were pre-incubated with NF-κB, JNK and PKC inhibitors and stimulated in 24-well plates with different concentrations of PMA, HK E. coli MG1655 and Calcium Ionophore A23187.
T207 1305-1547 Sentence denotes The cells were lysed and luciferase activity (NF-κB and AP-1) was measured using the Dual-Luciferase® reporter assay system (Promega, USA) according to the manufacturer's instructions on a TD 20/20 luminometer (Turner Designs, Sunnyvale, CA).
T208 1548-1680 Sentence denotes Secreted alkaline phosphatase (NFκB-SEAP, figure 4a, b) levels were measured using Great EscAPe™ SEAP Detection Kit (Clontech, USA).

events-check-again

Id Subject Object Predicate Lexical cue
T13194 1330-1340 Protein denotes luciferase
T13195 1341-1349 Positive_regulation denotes activity
R10640 T13194 T13195 themeOf luciferase,activity

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T12468 1330-1340 Protein denotes luciferase
T12469 1341-1349 Positive_regulation denotes activity
R9967 T12468 T12469 themeOf luciferase,activity

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T13140 1330-1340 P08659 denotes luciferase
T13141 1361-1365 P05412 denotes AP-1
T13142 1395-1405 P08659 denotes Luciferase

test2

Id Subject Object Predicate Lexical cue
T12466 1330-1340 Protein denotes luciferase