PMC:2874527 / 862-1663 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2874527","sourcedb":"PMC","sourceid":"2874527","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2874527","text":"Results\nDynamic metabolite data were collected from HepG2 cells after they had been deprived of extracellular glucose. The concentration of 25 extra- and intracellular intermediates was quantified using HPLC, LC-MS-MS, and GC-MS. The in silico metabolite dynamics were in accordance with the experimental data. The central carbon metabolism of hepatomas was further analyzed with a particular focus on the control of metabolite concentrations and metabolic fluxes. It was observed that the enzyme glucose-6-phosphate dehydrogenase exerted substantial negative control over the glycolytic flux, whereas oxidative phosphorylation had a significant positive control. The control over the rate of NADPH consumption was found to be shared between the NADPH-demand itself (0.65) and the NADPH supply (0.38).","divisions":[{"label":"Title","span":{"begin":0,"end":7}}],"tracks":[]}