> top > docs > PMC:2817507 > spans > 8506-9349 > annotations

PMC:2817507 / 8506-9349 JSONTXT

Annnotations TAB JSON ListView MergeView

2_test

Id Subject Object Predicate Lexical cue
20150961-17884182-67933707 529-530 17884182 denotes 8

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T4046 31-35 IN denotes from
T4045 22-30 VB denotes isolated
T4044 17-21 VB denotes were
T4043 8-16 NN denotes proteins
T4042 0-7 JJ denotes Nuclear
T4180 833-842 VB denotes described
T4179 822-832 RB denotes previously
T4178 819-821 IN denotes as
T4177 807-818 NN denotes specificity
T4176 799-806 NN denotes binding
T4175 794-798 NN denotes NFκB
T4174 786-793 VB denotes confirm
T4173 783-785 TO denotes to
T4172 773-782 VB denotes performed
T4171 769-772 VB denotes was
T4170 765-768 NN denotes P50
T4169 761-764 CC denotes and
T4168 757-760 NN denotes P65
T4167 754-756 TO denotes to
T4166 743-753 NN denotes antibodies
T4165 737-742 VB denotes using
T4164 731-736 NN denotes assay
T4163 725-730 NN denotes shift
T4162 719-724 JJ denotes super
T4161 717-718 DT denotes A
T4160 700-715 NN denotes oligonucleotide
T4159 690-699 NN denotes consensus
T4158 685-689 NN denotes NFκB
T4157 669-684 JJ denotes double-stranded
T4156 661-668 VB denotes labeled
T4155 659-660 -RRB- denotes ]
T4154 654-659 NN denotes γ-32P
T4153 653-654 -LRB- denotes [
T4152 645-652 NN denotes 35 fmol
T4151 641-644 CC denotes and
T4150 632-640 NN denotes proteins
T4149 624-631 JJ denotes nuclear
T4148 621-623 IN denotes of
T4147 615-620 NN denotes 15 μg
T4146 604-614 VB denotes containing
T4145 596-603 NN denotes mixture
T4144 587-595 NN denotes reaction
T4143 579-586 NN denotes binding
T4142 573-578 NN denotes 15 μL
T4141 571-572 DT denotes a
T4140 568-570 IN denotes in
T4139 559-567 VB denotes examined
T4138 555-558 VB denotes was
T4137 546-554 NN denotes activity
T4136 538-545 NN denotes binding
T4135 533-537 NN denotes NFκB
T4134 530-531 -RRB- denotes ]
T4133 529-530 CD denotes 8
T4132 528-529 -LRB- denotes [
T4131 517-527 RB denotes previously
T4130 507-516 VB denotes described
T4129 504-506 IN denotes as
T4128 494-503 VB denotes performed
T4127 490-493 VB denotes was
T4126 485-489 NN denotes EMSA
T4125 480-483 NN denotes use
T4124 474-479 IN denotes until
T4123 468-473 NN denotes −80°C
T4122 465-467 IN denotes at
T4121 458-464 VB denotes stored
T4120 454-457 CC denotes and
T4119 449-453 NN denotes tube
T4118 438-448 JJ denotes prechilled
T4117 432-437 JJ denotes clean
T4116 430-431 DT denotes a
T4115 427-429 TO denotes to
T4114 415-426 VB denotes transferred
T4113 410-414 VB denotes were
T4112 408-409 -RRB- denotes )
T4111 401-408 NN denotes extract
T4110 393-400 JJ denotes nuclear
T4109 392-393 -LRB- denotes (
T4108 379-391 NN denotes supernatants
T4107 375-378 DT denotes The
T4106 366-373 NN denotes minutes
T4105 363-365 CD denotes 10
T4104 359-362 IN denotes for
T4103 348-358 NN denotes centrifuge
T4102 342-347 NN denotes micro
T4101 340-341 DT denotes a
T4100 337-339 IN denotes in
T4099 335-336 -RRB- denotes )
T4098 332-335 NN denotes × g
T4097 324-331 CD denotes ~16,000
T4096 323-324 -LRB- denotes (
T4095 317-322 NN denotes speed
T4094 309-316 JJ denotes maximum
T4093 306-308 IN denotes at
T4092 294-305 VB denotes centrifuged
T4091 289-293 VB denotes were
T4090 283-288 NN denotes tubes
T4089 279-282 DT denotes The
T4088 270-277 NN denotes minutes
T4087 267-269 CD denotes 40
T4086 264-266 IN denotes of
T4085 258-263 NN denotes total
T4084 256-257 DT denotes a
T4083 252-255 IN denotes for
T4082 250-251 -COMMA- denotes ,
T4081 243-250 NN denotes minutes
T4080 240-242 CD denotes 10
T4079 234-239 DT denotes every
T4078 226-233 NN denotes seconds
T4077 223-225 CD denotes 15
T4076 219-222 IN denotes for
T4075 212-218 NN denotes vortex
T4074 209-211 IN denotes on
T4073 205-208 VB denotes put
T4072 200-204 VB denotes were
T4071 192-199 NN denotes pellets
T4070 181-191 VB denotes containing
T4069 175-180 NN denotes tubes
T4068 171-174 DT denotes The
T4067 162-169 NN denotes reagent
T4066 151-161 NN denotes extraction
T4065 143-150 JJ denotes nuclear
T4064 140-142 IN denotes in
T4063 128-139 VB denotes resuspended
T4062 123-127 VB denotes were
T4061 115-122 NN denotes pellets
T4060 111-114 DT denotes the
T4059 109-110 -COMMA- denotes ,
T4058 108-109 -RRB- denotes )
T4057 101-108 NN denotes extract
T4056 89-100 JJ denotes cytoplasmic
T4055 88-89 -LRB- denotes (
T4054 76-87 NN denotes supernatant
T4053 73-75 IN denotes of
T4052 65-72 NN denotes removal
T4051 59-64 IN denotes after
T4050 57-58 -COMMA- denotes ,
T4049 50-57 RB denotes Briefly
T4048 42-48 NN denotes tissue
T4047 36-41 NN denotes brain
R3115 T4043 T4042 arg1Of proteins,Nuclear
R3116 T4043 T4044 arg1Of proteins,were
R3117 T4043 T4045 arg2Of proteins,isolated
R3118 T4045 T4044 arg2Of isolated,were
R3119 T4045 T4046 arg1Of isolated,from
R3120 T4048 T4046 arg2Of tissue,from
R3121 T4048 T4047 arg1Of tissue,brain
R3122 T4052 T4051 arg2Of removal,after
R3123 T4052 T4053 arg1Of removal,of
R3124 T4054 T4053 arg2Of supernatant,of
R3125 T4054 T4055 arg1Of supernatant,(
R3126 T4057 T4055 arg2Of extract,(
R3127 T4057 T4056 arg1Of extract,cytoplasmic
R3128 T4058 T4055 arg3Of ),(
R3129 T4061 T4060 arg1Of pellets,the
R3130 T4061 T4062 arg1Of pellets,were
R3131 T4061 T4063 arg2Of pellets,resuspended
R3132 T4063 T4049 arg1Of resuspended,Briefly
R3133 T4063 T4050 arg1Of resuspended,","
R3134 T4063 T4051 arg1Of resuspended,after
R3135 T4063 T4059 arg1Of resuspended,","
R3136 T4063 T4062 arg2Of resuspended,were
R3137 T4063 T4064 arg1Of resuspended,in
R3138 T4067 T4064 arg2Of reagent,in
R3139 T4067 T4065 arg1Of reagent,nuclear
R3140 T4067 T4066 arg1Of reagent,extraction
R3141 T4069 T4068 arg1Of tubes,The
R3142 T4069 T4070 arg1Of tubes,containing
R3143 T4069 T4072 arg1Of tubes,were
R3144 T4069 T4073 arg2Of tubes,put
R3145 T4071 T4070 arg2Of pellets,containing
R3146 T4073 T4072 arg2Of put,were
R3147 T4073 T4074 arg1Of put,on
R3148 T4073 T4076 arg1Of put,for
R3149 T4073 T4081 arg1Of put,minutes
R3150 T4073 T4082 arg1Of put,","
R3151 T4073 T4083 arg1Of put,for
R3152 T4075 T4074 arg2Of vortex,on
R3153 T4078 T4076 arg2Of seconds,for
R3154 T4078 T4077 arg1Of seconds,15
R3155 T4081 T4079 arg1Of minutes,every
R3156 T4081 T4080 arg1Of minutes,10
R3157 T4085 T4083 arg2Of total,for
R3158 T4085 T4084 arg1Of total,a
R3159 T4085 T4086 arg1Of total,of
R3160 T4088 T4086 arg2Of minutes,of
R3161 T4088 T4087 arg1Of minutes,40
R3162 T4090 T4089 arg1Of tubes,The
R3163 T4090 T4091 arg1Of tubes,were
R3164 T4090 T4092 arg2Of tubes,centrifuged
R3165 T4092 T4091 arg2Of centrifuged,were
R3166 T4092 T4093 arg1Of centrifuged,at
R3167 T4095 T4093 arg2Of speed,at
R3168 T4095 T4094 arg1Of speed,maximum
R3169 T4095 T4096 arg1Of speed,(
R3170 T4095 T4100 arg1Of speed,in
R3171 T4098 T4096 arg2Of × g,(
R3172 T4098 T4097 arg1Of × g,"~16,000"
R3173 T4099 T4096 arg3Of ),(
R3174 T4103 T4100 arg2Of centrifuge,in
R3175 T4103 T4101 arg1Of centrifuge,a
R3176 T4103 T4102 arg1Of centrifuge,micro
R3177 T4103 T4104 arg1Of centrifuge,for
R3178 T4106 T4104 arg2Of minutes,for
R3179 T4106 T4105 arg1Of minutes,10
R3180 T4108 T4107 arg1Of supernatants,The
R3181 T4108 T4109 arg1Of supernatants,(
R3182 T4108 T4113 arg1Of supernatants,were
R3183 T4108 T4114 arg2Of supernatants,transferred
R3184 T4108 T4121 arg2Of supernatants,stored
R3185 T4111 T4109 arg2Of extract,(
R3186 T4111 T4110 arg1Of extract,nuclear
R3187 T4112 T4109 arg3Of ),(
R3188 T4114 T4115 arg1Of transferred,to
R3189 T4114 T4120 arg1Of transferred,and
R3190 T4119 T4115 arg2Of tube,to
R3191 T4119 T4116 arg1Of tube,a
R3192 T4119 T4117 arg1Of tube,clean
R3193 T4119 T4118 arg1Of tube,prechilled
R3194 T4120 T4113 arg2Of and,were
R3195 T4121 T4120 arg2Of stored,and
R3196 T4121 T4122 arg1Of stored,at
R3197 T4121 T4124 arg1Of stored,until
R3198 T4123 T4122 arg2Of −80°C,at
R3199 T4125 T4124 arg2Of use,until
R3200 T4126 T4127 arg1Of EMSA,was
R3201 T4126 T4128 arg2Of EMSA,performed
R3202 T4128 T4127 arg2Of performed,was
R3203 T4128 T4129 arg1Of performed,as
R3204 T4128 T4132 arg1Of performed,[
R3205 T4130 T4129 arg2Of described,as
R3206 T4130 T4131 arg1Of described,previously
R3207 T4133 T4132 arg2Of 8,[
R3208 T4134 T4132 arg3Of ],[
R3209 T4137 T4135 arg1Of activity,NFκB
R3210 T4137 T4136 arg1Of activity,binding
R3211 T4137 T4138 arg1Of activity,was
R3212 T4137 T4139 arg2Of activity,examined
R3213 T4139 T4138 arg2Of examined,was
R3214 T4139 T4140 arg1Of examined,in
R3215 T4145 T4140 arg2Of mixture,in
R3216 T4145 T4141 arg1Of mixture,a
R3217 T4145 T4142 arg1Of mixture,15 μL
R3218 T4145 T4143 arg1Of mixture,binding
R3219 T4145 T4144 arg1Of mixture,reaction
R3220 T4145 T4146 arg1Of mixture,containing
R3221 T4147 T4148 arg1Of 15 μg,of
R3222 T4147 T4151 arg1Of 15 μg,and
R3223 T4150 T4148 arg2Of proteins,of
R3224 T4150 T4149 arg1Of proteins,nuclear
R3225 T4151 T4146 arg2Of and,containing
R3226 T4152 T4153 arg1Of 35 fmol,[
R3227 T4154 T4153 arg2Of γ-32P,[
R3228 T4155 T4153 arg3Of ],[
R3229 T4160 T4151 arg2Of oligonucleotide,and
R3230 T4160 T4152 arg1Of oligonucleotide,35 fmol
R3231 T4160 T4156 arg2Of oligonucleotide,labeled
R3232 T4160 T4157 arg1Of oligonucleotide,double-stranded
R3233 T4160 T4158 arg1Of oligonucleotide,NFκB
R3234 T4160 T4159 arg1Of oligonucleotide,consensus
R3235 T4164 T4161 arg1Of assay,A
R3236 T4164 T4162 arg1Of assay,super
R3237 T4164 T4163 arg1Of assay,shift
R3238 T4164 T4165 arg1Of assay,using
R3239 T4164 T4171 arg1Of assay,was
R3240 T4164 T4172 arg2Of assay,performed
R3241 T4166 T4165 arg2Of antibodies,using
R3242 T4166 T4167 arg1Of antibodies,to
R3243 T4168 T4169 arg1Of P65,and
R3244 T4169 T4167 arg2Of and,to
R3245 T4170 T4169 arg2Of P50,and
R3246 T4172 T4171 arg2Of performed,was
R3247 T4172 T4173 modOf performed,to
R3248 T4174 T4173 arg1Of confirm,to
R3249 T4177 T4174 arg2Of specificity,confirm
R3250 T4177 T4175 arg1Of specificity,NFκB
R3251 T4177 T4176 arg1Of specificity,binding
R3252 T4177 T4178 arg1Of specificity,as
R3253 T4180 T4178 arg2Of described,as
R3254 T4180 T4179 arg1Of described,previously

bionlp-st-ge-2016-test-proteins

Id Subject Object Predicate Lexical cue
T4019 765-768 Protein denotes P50
T4018 757-760 Protein denotes P65

UBERON-AE

Id Subject Object Predicate Lexical cue
T4005 449-453 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tube
T4004 283-288 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tubes
T4003 175-180 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tubes
T4002 42-48 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
T4001 36-41 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain

GO-MF

Id Subject Object Predicate Lexical cue
T4023 743-753 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T4022 799-806 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T4021 579-586 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T4020 538-545 http://purl.obolibrary.org/obo/GO_0005488 denotes binding

GO-CC

Id Subject Object Predicate Lexical cue
T4025 743-753 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T4024 743-753 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies

sentences

Id Subject Object Predicate Lexical cue
T4015 717-843 Sentence denotes A super shift assay using antibodies to P65 and P50 was performed to confirm NFκB binding specificity as previously described.
T4014 533-716 Sentence denotes NFκB binding activity was examined in a 15 μL binding reaction mixture containing 15 μg of nuclear proteins and 35 fmol [γ-32P] labeled double-stranded NFκB consensus oligonucleotide.
T4013 485-532 Sentence denotes EMSA was performed as described previously [8].
T4012 375-484 Sentence denotes The supernatants (nuclear extract) were transferred to a clean prechilled tube and stored at −80°C until use.
T4011 279-374 Sentence denotes The tubes were centrifuged at maximum speed (~16,000 × g) in a micro centrifuge for 10 minutes.
T4010 171-278 Sentence denotes The tubes containing pellets were put on vortex for 15 seconds every 10 minutes, for a total of 40 minutes.
T4009 50-170 Sentence denotes Briefly, after removal of supernatant (cytoplasmic extract), the pellets were resuspended in nuclear extraction reagent.
T4008 0-49 Sentence denotes Nuclear proteins were isolated from brain tissue.
T86 0-49 Sentence denotes Nuclear proteins were isolated from brain tissue.
T87 50-170 Sentence denotes Briefly, after removal of supernatant (cytoplasmic extract), the pellets were resuspended in nuclear extraction reagent.
T88 171-278 Sentence denotes The tubes containing pellets were put on vortex for 15 seconds every 10 minutes, for a total of 40 minutes.
T89 279-374 Sentence denotes The tubes were centrifuged at maximum speed (~16,000 × g) in a micro centrifuge for 10 minutes.
T90 375-484 Sentence denotes The supernatants (nuclear extract) were transferred to a clean prechilled tube and stored at −80°C until use.
T91 485-532 Sentence denotes EMSA was performed as described previously [8].
T92 533-716 Sentence denotes NFκB binding activity was examined in a 15 μL binding reaction mixture containing 15 μg of nuclear proteins and 35 fmol [γ-32P] labeled double-stranded NFκB consensus oligonucleotide.
T93 717-843 Sentence denotes A super shift assay using antibodies to P65 and P50 was performed to confirm NFκB binding specificity as previously described.

simple1

Id Subject Object Predicate Lexical cue
T4033 765-768 Protein denotes P50
T4032 757-760 Protein denotes P65

BioNLP16_DUT

Id Subject Object Predicate Lexical cue
T4360 765-768 Protein denotes P50
T4359 757-760 Protein denotes P65

BioNLP16_Messiy

Id Subject Object Predicate Lexical cue
T4346 765-768 Protein denotes P50
T4345 757-760 Protein denotes P65

DLUT931

Id Subject Object Predicate Lexical cue
T4350 765-768 Protein denotes P50
T4349 757-760 Protein denotes P65

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T4377 765-768 Protein denotes P50
T4376 393-408 Entity denotes nuclear extract
T4375 279-288 Protein denotes The tubes
T4374 757-760 Protein denotes P65
T4373 669-689 Protein denotes double-stranded NFκB
T4372 171-199 Protein denotes The tubes containing pellets
T4371 587-614 Entity denotes reaction mixture containing
T4370 690-715 Entity denotes consensus oligonucleotide
T4369 624-640 Protein denotes nuclear proteins
T4368 375-409 Entity denotes The supernatants (nuclear extract)
T4367 143-169 Entity denotes nuclear extraction reagent
T4366 212-218 Entity denotes vortex
T4365 0-16 Protein denotes Nuclear proteins
T4364 73-109 Entity denotes of supernatant (cytoplasmic extract)
T4363 89-108 Entity denotes cytoplasmic extract
T4362 449-483 Protein denotes tube and stored at −80°C until use
T4361 743-753 Entity denotes antibodies

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T4344 842-843 . denotes .
T4343 833-842 VBN denotes described
T4342 822-832 RB denotes previously
T4341 819-821 IN denotes as
T4340 807-818 NN denotes specificity
T4339 799-806 JJ denotes binding
T4338 794-798 NNP denotes NFκB
T4337 786-793 VB denotes confirm
T4336 783-785 TO denotes to
T4335 773-782 VBN denotes performed
T4334 769-772 VBD denotes was
T4333 765-768 NNP denotes P50
T4332 761-764 CC denotes and
T4331 757-760 NNP denotes P65
T4330 754-756 TO denotes to
T4329 743-753 NNS denotes antibodies
T4328 737-742 VBG denotes using
T4327 731-736 NN denotes assay
T4326 725-730 NN denotes shift
T4325 719-724 JJ denotes super
T4324 717-718 DT denotes A
T4323 715-716 . denotes .
T4322 700-715 NN denotes oligonucleotide
T4321 690-699 NN denotes consensus
T4320 685-689 NNP denotes NFκB
T4319 669-684 JJ denotes double-stranded
T4318 661-668 VBD denotes labeled
T4317 659-660 NNP denotes ]
T4316 654-659 NNP denotes γ-32P
T4315 653-654 NNP denotes [
T4314 648-652 NN denotes fmol
T4313 645-647 CD denotes 35
T4312 641-644 CC denotes and
T4311 632-640 NNS denotes proteins
T4310 624-631 JJ denotes nuclear
T4309 621-623 IN denotes of
T4308 618-620 NN denotes μg
T4307 615-617 CD denotes 15
T4306 604-614 VBG denotes containing
T4305 596-603 NN denotes mixture
T4304 587-595 NN denotes reaction
T4303 579-586 JJ denotes binding
T4302 576-578 NN denotes μL
T4301 573-575 CD denotes 15
T4300 571-572 DT denotes a
T4299 568-570 IN denotes in
T4298 559-567 VBN denotes examined
T4297 555-558 VBD denotes was
T4296 546-554 NN denotes activity
T4295 538-545 JJ denotes binding
T4294 533-537 NNP denotes NFκB
T4293 531-532 . denotes .
T4292 530-531 NN denotes ]
T4291 529-530 CD denotes 8
T4290 528-529 JJ denotes [
T4289 517-527 RB denotes previously
T4288 507-516 VBN denotes described
T4287 504-506 IN denotes as
T4286 494-503 VBN denotes performed
T4285 490-493 VBD denotes was
T4284 485-489 NNP denotes EMSA
T4283 483-484 . denotes .
T4282 480-483 NN denotes use
T4281 474-479 IN denotes until
T4280 472-473 NNP denotes C
T4279 471-472 CD denotes °
T4278 469-471 CD denotes 80
T4277 468-469 CD denotes −
T4276 465-467 IN denotes at
T4275 458-464 VBD denotes stored
T4274 454-457 CC denotes and
T4273 449-453 NN denotes tube
T4272 438-448 JJ denotes prechilled
T4271 432-437 JJ denotes clean
T4270 430-431 DT denotes a
T4269 427-429 TO denotes to
T4268 415-426 VBN denotes transferred
T4267 410-414 VBD denotes were
T4266 408-409 -RRB- denotes )
T4265 401-408 VB denotes extract
T4264 393-400 JJ denotes nuclear
T4263 392-393 -LRB- denotes (
T4262 379-391 NNS denotes supernatants
T4261 375-378 DT denotes The
T4260 373-374 . denotes .
T4259 366-373 NNS denotes minutes
T4258 363-365 CD denotes 10
T4257 359-362 IN denotes for
T4256 348-358 NN denotes centrifuge
T4255 342-347 NN denotes micro
T4254 340-341 DT denotes a
T4253 337-339 IN denotes in
T4252 335-336 -RRB- denotes )
T4251 334-335 NN denotes g
T4250 332-333 CD denotes ×
T4249 325-331 CD denotes 16,000
T4248 324-325 FW denotes ~
T4247 323-324 -LRB- denotes (
T4246 317-322 NN denotes speed
T4245 309-316 JJ denotes maximum
T4244 306-308 IN denotes at
T4243 294-305 VBN denotes centrifuged
T4242 289-293 VBD denotes were
T4241 283-288 NNS denotes tubes
T4240 279-282 DT denotes The
T4218 171-174 DT denotes The
T4217 169-170 . denotes .
T4216 162-169 NN denotes reagent
T4215 151-161 NN denotes extraction
T4214 143-150 JJ denotes nuclear
T4213 140-142 IN denotes in
T4212 128-139 VBN denotes resuspended
T4211 123-127 VBD denotes were
T4210 115-122 NNS denotes pellets
T4209 111-114 DT denotes the
T4208 109-110 , denotes ,
T4207 108-109 -RRB- denotes )
T4206 101-108 VB denotes extract
T4205 89-100 JJ denotes cytoplasmic
T4204 88-89 -LRB- denotes (
T4203 76-87 JJ denotes supernatant
T4202 73-75 IN denotes of
T4201 65-72 NN denotes removal
T4200 59-64 IN denotes after
T4199 57-58 , denotes ,
T4198 50-57 RB denotes Briefly
T4197 48-49 . denotes .
T4196 42-48 NN denotes tissue
T4195 36-41 NN denotes brain
T4194 31-35 IN denotes from
T4193 22-30 VBN denotes isolated
T4192 17-21 VBD denotes were
T4191 8-16 NNS denotes proteins
T4190 0-7 NNP denotes Nuclear
T4239 277-278 . denotes .
T4238 270-277 NNS denotes minutes
T4237 267-269 CD denotes 40
T4236 264-266 IN denotes of
T4235 258-263 NN denotes total
T4234 256-257 DT denotes a
T4233 252-255 IN denotes for
T4232 250-251 , denotes ,
T4231 243-250 NNS denotes minutes
T4230 240-242 CD denotes 10
T4229 234-239 DT denotes every
T4228 226-233 NNS denotes seconds
T4227 223-225 CD denotes 15
T4226 219-222 IN denotes for
T4225 212-218 NN denotes vortex
T4224 209-211 IN denotes on
T4223 205-208 VBN denotes put
T4222 200-204 VBD denotes were
T4221 192-199 NNS denotes pellets
T4220 181-191 VBG denotes containing
T4219 175-180 NNS denotes tubes
R3278 T4204 T4206 punct (,extract
R3279 T4205 T4206 nsubj cytoplasmic,extract
R3280 T4206 T4206 ROOT extract,extract
R3281 T4207 T4206 punct ),extract
R3282 T4208 T4212 punct ",",resuspended
R3283 T4209 T4210 det the,pellets
R3284 T4210 T4212 nsubjpass pellets,resuspended
R3285 T4211 T4212 auxpass were,resuspended
R3286 T4212 T4212 ROOT resuspended,resuspended
R3287 T4213 T4212 prep in,resuspended
R3288 T4214 T4215 amod nuclear,extraction
R3289 T4215 T4213 pobj extraction,in
R3290 T4216 T4212 conj reagent,resuspended
R3291 T4217 T4212 punct .,resuspended
R3292 T4218 T4219 det The,tubes
R3293 T4219 T4223 nsubjpass tubes,put
R3294 T4220 T4219 acl containing,tubes
R3295 T4221 T4220 dobj pellets,containing
R3296 T4222 T4223 auxpass were,put
R3297 T4223 T4223 ROOT put,put
R3298 T4224 T4223 prep on,put
R3299 T4225 T4224 pobj vortex,on
R3300 T4226 T4223 prep for,put
R3301 T4227 T4228 nummod 15,seconds
R3302 T4228 T4226 pobj seconds,for
R3303 T4229 T4231 det every,minutes
R3304 T4230 T4231 nummod 10,minutes
R3305 T4231 T4223 npadvmod minutes,put
R3306 T4232 T4231 punct ",",minutes
R3307 T4233 T4231 prep for,minutes
R3308 T4234 T4235 det a,total
R3309 T4235 T4233 pobj total,for
R3310 T4236 T4235 prep of,total
R3311 T4237 T4238 nummod 40,minutes
R3312 T4238 T4236 pobj minutes,of
R3313 T4239 T4223 punct .,put
R3314 T4240 T4241 det The,tubes
R3315 T4241 T4243 nsubjpass tubes,centrifuged
R3316 T4242 T4243 auxpass were,centrifuged
R3317 T4243 T4243 ROOT centrifuged,centrifuged
R3318 T4244 T4243 prep at,centrifuged
R3319 T4245 T4246 amod maximum,speed
R3320 T4246 T4244 pobj speed,at
R3321 T4247 T4251 punct (,g
R3322 T4248 T4251 nmod ~,g
R3323 T4249 T4251 dep "16,000",g
R3324 T4250 T4251 nummod ×,g
R3325 T4251 T4246 appos g,speed
R3326 T4252 T4251 punct ),g
R3327 T4253 T4243 prep in,centrifuged
R3328 T4254 T4256 det a,centrifuge
R3329 T4255 T4256 amod micro,centrifuge
R3330 T4256 T4253 pobj centrifuge,in
R3331 T4257 T4243 prep for,centrifuged
R3332 T4258 T4259 nummod 10,minutes
R3333 T4259 T4257 pobj minutes,for
R3334 T4260 T4243 punct .,centrifuged
R3335 T4261 T4262 det The,supernatants
R3336 T4262 T4268 nsubjpass supernatants,transferred
R3337 T4263 T4262 punct (,supernatants
R3338 T4264 T4265 amod nuclear,extract
R3339 T4265 T4262 appos extract,supernatants
R3340 T4266 T4262 punct ),supernatants
R3341 T4267 T4268 auxpass were,transferred
R3342 T4268 T4268 ROOT transferred,transferred
R3343 T4269 T4268 prep to,transferred
R3344 T4270 T4273 det a,tube
R3345 T4271 T4273 amod clean,tube
R3346 T4272 T4273 amod prechilled,tube
R3347 T4273 T4269 pobj tube,to
R3348 T4274 T4268 cc and,transferred
R3349 T4275 T4268 conj stored,transferred
R3350 T4276 T4275 prep at,stored
R3351 T4277 T4279 nummod −,°
R3352 T4278 T4279 nummod 80,°
R3353 T4279 T4276 pobj °,at
R3354 T4280 T4275 npadvmod C,stored
R3355 T4281 T4275 prep until,stored
R3356 T4282 T4281 pobj use,until
R3357 T4283 T4268 punct .,transferred
R3358 T4284 T4286 nsubjpass EMSA,performed
R3359 T4285 T4286 auxpass was,performed
R3360 T4286 T4286 ROOT performed,performed
R3361 T4287 T4288 mark as,described
R3362 T4288 T4286 advcl described,performed
R3363 T4289 T4290 advmod previously,[
R3364 T4290 T4292 amod [,]
R3365 T4291 T4292 nummod 8,]
R3366 T4292 T4288 dobj ],described
R3367 T4293 T4286 punct .,performed
R3368 T4294 T4296 amod NFκB,activity
R3369 T4295 T4296 amod binding,activity
R3370 T4296 T4298 nsubjpass activity,examined
R3371 T4297 T4298 auxpass was,examined
R3372 T4298 T4298 ROOT examined,examined
R3373 T4299 T4298 prep in,examined
R3374 T4300 T4305 det a,mixture
R3375 T4301 T4302 nummod 15,μL
R3376 T4302 T4305 compound μL,mixture
R3377 T4303 T4304 amod binding,reaction
R3378 T4304 T4305 compound reaction,mixture
R3379 T4305 T4299 pobj mixture,in
R3380 T4306 T4305 acl containing,mixture
R3381 T4307 T4308 nummod 15,μg
R3382 T4308 T4317 nmod μg,]
R3383 T4309 T4308 prep of,μg
R3384 T4310 T4311 amod nuclear,proteins
R3385 T4311 T4309 pobj proteins,of
R3386 T4312 T4311 cc and,proteins
R3387 T4313 T4314 nummod 35,fmol
R3388 T4314 T4317 compound fmol,]
R3389 T4315 T4317 compound [,]
R3390 T4316 T4317 compound γ-32P,]
R3391 T4317 T4306 dobj ],containing
R3392 T4318 T4298 conj labeled,examined
R3393 T4319 T4322 amod double-stranded,oligonucleotide
R3394 T4320 T4321 compound NFκB,consensus
R3395 T4321 T4322 compound consensus,oligonucleotide
R3396 T4322 T4318 oprd oligonucleotide,labeled
R3397 T4323 T4298 punct .,examined
R3398 T4324 T4327 det A,assay
R3399 T4325 T4326 amod super,shift
R3400 T4326 T4327 compound shift,assay
R3401 T4327 T4335 nsubjpass assay,performed
R3402 T4328 T4327 acl using,assay
R3403 T4329 T4328 dobj antibodies,using
R3404 T4330 T4328 prep to,using
R3405 T4331 T4330 pobj P65,to
R3406 T4332 T4331 cc and,P65
R3407 T4333 T4331 conj P50,P65
R3408 T4334 T4335 auxpass was,performed
R3409 T4335 T4335 ROOT performed,performed
R3410 T4336 T4337 aux to,confirm
R3411 T4337 T4335 xcomp confirm,performed
R3412 T4338 T4340 nmod NFκB,specificity
R3413 T4339 T4340 amod binding,specificity
R3414 T4340 T4337 dobj specificity,confirm
R3415 T4341 T4343 mark as,described
R3416 T4342 T4343 advmod previously,described
R3417 T4343 T4340 advcl described,specificity
R3418 T4344 T4335 punct .,performed
R3264 T4190 T4191 compound Nuclear,proteins
R3265 T4191 T4192 nsubj proteins,were
R3266 T4192 T4193 auxpass were,isolated
R3267 T4193 T4193 ROOT isolated,isolated
R3268 T4194 T4193 prep from,isolated
R3269 T4195 T4196 compound brain,tissue
R3270 T4196 T4194 pobj tissue,from
R3271 T4197 T4193 punct .,isolated
R3272 T4198 T4206 advmod Briefly,extract
R3273 T4199 T4206 punct ",",extract
R3274 T4200 T4206 prep after,extract
R3275 T4201 T4200 pobj removal,after
R3276 T4202 T4201 prep of,removal
R3277 T4203 T4202 pobj supernatant,of

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T4031 794-798 Protein denotes NFκB
T4030 765-768 Protein denotes P50
T4029 757-760 Protein denotes P65
T4028 538-545 Binding denotes binding
T4027 669-715 Protein denotes double-stranded NFκB consensus oligonucleotide
T4026 533-537 Protein denotes NFκB
R3108 T4026 T4028 themeOf NFκB,binding

testone

Id Subject Object Predicate Lexical cue
T3995 799-806 Binding denotes binding
T3994 765-768 Protein denotes P50
T3993 757-760 Protein denotes P65
R3104 T3993 T3995 themeOf P65,binding
R3105 T3994 T3995 themeOf P50,binding

test3

Id Subject Object Predicate Lexical cue
T4000 799-806 Binding denotes binding
T3999 765-768 Protein denotes P50
T3998 757-760 Protein denotes P65
T3997 765-768 Protein denotes P50
T3996 757-760 Protein denotes P65
R3106 T3998 T4000 themeOf P65,binding
R3107 T3999 T4000 themeOf P50,binding