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PMC:2817507 / 8457-9349 JSONTXT

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2_test

Id Subject Object Predicate Lexical cue
20150961-17884182-67933707 578-579 17884182 denotes 8

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T4046 80-84 IN denotes from
T4045 71-79 VB denotes isolated
T4044 66-70 VB denotes were
T4043 57-65 NN denotes proteins
T4042 49-56 JJ denotes Nuclear
T4041 47-48 -RRB- denotes )
T4040 43-47 NN denotes EMSA
T4039 42-43 -LRB- denotes (
T4038 36-41 NN denotes Assay
T4037 30-35 NN denotes Shift
T4036 21-29 NN denotes Mobility
T4035 5-20 JJ denotes Electrophoretic
T4034 0-3 CD denotes 2.6
T4180 882-891 VB denotes described
T4179 871-881 RB denotes previously
T4178 868-870 IN denotes as
T4177 856-867 NN denotes specificity
T4176 848-855 NN denotes binding
T4175 843-847 NN denotes NFκB
T4174 835-842 VB denotes confirm
T4173 832-834 TO denotes to
T4172 822-831 VB denotes performed
T4171 818-821 VB denotes was
T4170 814-817 NN denotes P50
T4169 810-813 CC denotes and
T4168 806-809 NN denotes P65
T4167 803-805 TO denotes to
T4166 792-802 NN denotes antibodies
T4165 786-791 VB denotes using
T4164 780-785 NN denotes assay
T4163 774-779 NN denotes shift
T4162 768-773 JJ denotes super
T4161 766-767 DT denotes A
T4160 749-764 NN denotes oligonucleotide
T4159 739-748 NN denotes consensus
T4158 734-738 NN denotes NFκB
T4157 718-733 JJ denotes double-stranded
T4156 710-717 VB denotes labeled
T4155 708-709 -RRB- denotes ]
T4154 703-708 NN denotes γ-32P
T4153 702-703 -LRB- denotes [
T4152 694-701 NN denotes 35 fmol
T4151 690-693 CC denotes and
T4150 681-689 NN denotes proteins
T4149 673-680 JJ denotes nuclear
T4148 670-672 IN denotes of
T4147 664-669 NN denotes 15 μg
T4146 653-663 VB denotes containing
T4145 645-652 NN denotes mixture
T4144 636-644 NN denotes reaction
T4143 628-635 NN denotes binding
T4142 622-627 NN denotes 15 μL
T4141 620-621 DT denotes a
T4140 617-619 IN denotes in
T4139 608-616 VB denotes examined
T4138 604-607 VB denotes was
T4137 595-603 NN denotes activity
T4136 587-594 NN denotes binding
T4135 582-586 NN denotes NFκB
T4134 579-580 -RRB- denotes ]
T4133 578-579 CD denotes 8
T4132 577-578 -LRB- denotes [
T4131 566-576 RB denotes previously
T4130 556-565 VB denotes described
T4129 553-555 IN denotes as
T4128 543-552 VB denotes performed
T4127 539-542 VB denotes was
T4126 534-538 NN denotes EMSA
T4125 529-532 NN denotes use
T4124 523-528 IN denotes until
T4123 517-522 NN denotes −80°C
T4122 514-516 IN denotes at
T4121 507-513 VB denotes stored
T4120 503-506 CC denotes and
T4119 498-502 NN denotes tube
T4118 487-497 JJ denotes prechilled
T4117 481-486 JJ denotes clean
T4116 479-480 DT denotes a
T4115 476-478 TO denotes to
T4114 464-475 VB denotes transferred
T4113 459-463 VB denotes were
T4112 457-458 -RRB- denotes )
T4111 450-457 NN denotes extract
T4110 442-449 JJ denotes nuclear
T4109 441-442 -LRB- denotes (
T4108 428-440 NN denotes supernatants
T4107 424-427 DT denotes The
T4106 415-422 NN denotes minutes
T4105 412-414 CD denotes 10
T4104 408-411 IN denotes for
T4103 397-407 NN denotes centrifuge
T4102 391-396 NN denotes micro
T4101 389-390 DT denotes a
T4100 386-388 IN denotes in
T4099 384-385 -RRB- denotes )
T4098 381-384 NN denotes × g
T4097 373-380 CD denotes ~16,000
T4096 372-373 -LRB- denotes (
T4095 366-371 NN denotes speed
T4094 358-365 JJ denotes maximum
T4093 355-357 IN denotes at
T4092 343-354 VB denotes centrifuged
T4091 338-342 VB denotes were
T4090 332-337 NN denotes tubes
T4089 328-331 DT denotes The
T4088 319-326 NN denotes minutes
T4087 316-318 CD denotes 40
T4086 313-315 IN denotes of
T4085 307-312 NN denotes total
T4084 305-306 DT denotes a
T4083 301-304 IN denotes for
T4082 299-300 -COMMA- denotes ,
T4081 292-299 NN denotes minutes
T4080 289-291 CD denotes 10
T4079 283-288 DT denotes every
T4078 275-282 NN denotes seconds
T4077 272-274 CD denotes 15
T4076 268-271 IN denotes for
T4075 261-267 NN denotes vortex
T4074 258-260 IN denotes on
T4073 254-257 VB denotes put
T4072 249-253 VB denotes were
T4071 241-248 NN denotes pellets
T4070 230-240 VB denotes containing
T4069 224-229 NN denotes tubes
T4068 220-223 DT denotes The
T4067 211-218 NN denotes reagent
T4066 200-210 NN denotes extraction
T4065 192-199 JJ denotes nuclear
T4064 189-191 IN denotes in
T4063 177-188 VB denotes resuspended
T4062 172-176 VB denotes were
T4061 164-171 NN denotes pellets
T4060 160-163 DT denotes the
T4059 158-159 -COMMA- denotes ,
T4058 157-158 -RRB- denotes )
T4057 150-157 NN denotes extract
T4056 138-149 JJ denotes cytoplasmic
T4055 137-138 -LRB- denotes (
T4054 125-136 NN denotes supernatant
T4053 122-124 IN denotes of
T4052 114-121 NN denotes removal
T4051 108-113 IN denotes after
T4050 106-107 -COMMA- denotes ,
T4049 99-106 RB denotes Briefly
T4048 91-97 NN denotes tissue
T4047 85-90 NN denotes brain
R3109 T4038 T4035 arg1Of Assay,Electrophoretic
R3110 T4038 T4036 arg1Of Assay,Mobility
R3111 T4038 T4037 arg1Of Assay,Shift
R3112 T4038 T4039 arg1Of Assay,(
R3113 T4040 T4039 arg2Of EMSA,(
R3114 T4041 T4039 arg3Of ),(
R3115 T4043 T4042 arg1Of proteins,Nuclear
R3116 T4043 T4044 arg1Of proteins,were
R3117 T4043 T4045 arg2Of proteins,isolated
R3118 T4045 T4044 arg2Of isolated,were
R3119 T4045 T4046 arg1Of isolated,from
R3120 T4048 T4046 arg2Of tissue,from
R3121 T4048 T4047 arg1Of tissue,brain
R3122 T4052 T4051 arg2Of removal,after
R3123 T4052 T4053 arg1Of removal,of
R3124 T4054 T4053 arg2Of supernatant,of
R3125 T4054 T4055 arg1Of supernatant,(
R3126 T4057 T4055 arg2Of extract,(
R3127 T4057 T4056 arg1Of extract,cytoplasmic
R3128 T4058 T4055 arg3Of ),(
R3129 T4061 T4060 arg1Of pellets,the
R3130 T4061 T4062 arg1Of pellets,were
R3131 T4061 T4063 arg2Of pellets,resuspended
R3132 T4063 T4049 arg1Of resuspended,Briefly
R3133 T4063 T4050 arg1Of resuspended,","
R3134 T4063 T4051 arg1Of resuspended,after
R3135 T4063 T4059 arg1Of resuspended,","
R3136 T4063 T4062 arg2Of resuspended,were
R3137 T4063 T4064 arg1Of resuspended,in
R3138 T4067 T4064 arg2Of reagent,in
R3139 T4067 T4065 arg1Of reagent,nuclear
R3140 T4067 T4066 arg1Of reagent,extraction
R3141 T4069 T4068 arg1Of tubes,The
R3142 T4069 T4070 arg1Of tubes,containing
R3143 T4069 T4072 arg1Of tubes,were
R3144 T4069 T4073 arg2Of tubes,put
R3145 T4071 T4070 arg2Of pellets,containing
R3146 T4073 T4072 arg2Of put,were
R3147 T4073 T4074 arg1Of put,on
R3148 T4073 T4076 arg1Of put,for
R3149 T4073 T4081 arg1Of put,minutes
R3150 T4073 T4082 arg1Of put,","
R3151 T4073 T4083 arg1Of put,for
R3152 T4075 T4074 arg2Of vortex,on
R3153 T4078 T4076 arg2Of seconds,for
R3154 T4078 T4077 arg1Of seconds,15
R3155 T4081 T4079 arg1Of minutes,every
R3156 T4081 T4080 arg1Of minutes,10
R3157 T4085 T4083 arg2Of total,for
R3158 T4085 T4084 arg1Of total,a
R3159 T4085 T4086 arg1Of total,of
R3160 T4088 T4086 arg2Of minutes,of
R3161 T4088 T4087 arg1Of minutes,40
R3162 T4090 T4089 arg1Of tubes,The
R3163 T4090 T4091 arg1Of tubes,were
R3164 T4090 T4092 arg2Of tubes,centrifuged
R3165 T4092 T4091 arg2Of centrifuged,were
R3166 T4092 T4093 arg1Of centrifuged,at
R3167 T4095 T4093 arg2Of speed,at
R3168 T4095 T4094 arg1Of speed,maximum
R3169 T4095 T4096 arg1Of speed,(
R3170 T4095 T4100 arg1Of speed,in
R3171 T4098 T4096 arg2Of × g,(
R3172 T4098 T4097 arg1Of × g,"~16,000"
R3173 T4099 T4096 arg3Of ),(
R3174 T4103 T4100 arg2Of centrifuge,in
R3175 T4103 T4101 arg1Of centrifuge,a
R3176 T4103 T4102 arg1Of centrifuge,micro
R3177 T4103 T4104 arg1Of centrifuge,for
R3178 T4106 T4104 arg2Of minutes,for
R3179 T4106 T4105 arg1Of minutes,10
R3180 T4108 T4107 arg1Of supernatants,The
R3181 T4108 T4109 arg1Of supernatants,(
R3182 T4108 T4113 arg1Of supernatants,were
R3183 T4108 T4114 arg2Of supernatants,transferred
R3184 T4108 T4121 arg2Of supernatants,stored
R3185 T4111 T4109 arg2Of extract,(
R3186 T4111 T4110 arg1Of extract,nuclear
R3187 T4112 T4109 arg3Of ),(
R3188 T4114 T4115 arg1Of transferred,to
R3189 T4114 T4120 arg1Of transferred,and
R3190 T4119 T4115 arg2Of tube,to
R3191 T4119 T4116 arg1Of tube,a
R3192 T4119 T4117 arg1Of tube,clean
R3193 T4119 T4118 arg1Of tube,prechilled
R3194 T4120 T4113 arg2Of and,were
R3195 T4121 T4120 arg2Of stored,and
R3196 T4121 T4122 arg1Of stored,at
R3197 T4121 T4124 arg1Of stored,until
R3198 T4123 T4122 arg2Of −80°C,at
R3199 T4125 T4124 arg2Of use,until
R3200 T4126 T4127 arg1Of EMSA,was
R3201 T4126 T4128 arg2Of EMSA,performed
R3202 T4128 T4127 arg2Of performed,was
R3203 T4128 T4129 arg1Of performed,as
R3204 T4128 T4132 arg1Of performed,[
R3205 T4130 T4129 arg2Of described,as
R3206 T4130 T4131 arg1Of described,previously
R3207 T4133 T4132 arg2Of 8,[
R3208 T4134 T4132 arg3Of ],[
R3209 T4137 T4135 arg1Of activity,NFκB
R3210 T4137 T4136 arg1Of activity,binding
R3211 T4137 T4138 arg1Of activity,was
R3212 T4137 T4139 arg2Of activity,examined
R3213 T4139 T4138 arg2Of examined,was
R3214 T4139 T4140 arg1Of examined,in
R3215 T4145 T4140 arg2Of mixture,in
R3216 T4145 T4141 arg1Of mixture,a
R3217 T4145 T4142 arg1Of mixture,15 μL
R3218 T4145 T4143 arg1Of mixture,binding
R3219 T4145 T4144 arg1Of mixture,reaction
R3220 T4145 T4146 arg1Of mixture,containing
R3221 T4147 T4148 arg1Of 15 μg,of
R3222 T4147 T4151 arg1Of 15 μg,and
R3223 T4150 T4148 arg2Of proteins,of
R3224 T4150 T4149 arg1Of proteins,nuclear
R3225 T4151 T4146 arg2Of and,containing
R3226 T4152 T4153 arg1Of 35 fmol,[
R3227 T4154 T4153 arg2Of γ-32P,[
R3228 T4155 T4153 arg3Of ],[
R3229 T4160 T4151 arg2Of oligonucleotide,and
R3230 T4160 T4152 arg1Of oligonucleotide,35 fmol
R3231 T4160 T4156 arg2Of oligonucleotide,labeled
R3232 T4160 T4157 arg1Of oligonucleotide,double-stranded
R3233 T4160 T4158 arg1Of oligonucleotide,NFκB
R3234 T4160 T4159 arg1Of oligonucleotide,consensus
R3235 T4164 T4161 arg1Of assay,A
R3236 T4164 T4162 arg1Of assay,super
R3237 T4164 T4163 arg1Of assay,shift
R3238 T4164 T4165 arg1Of assay,using
R3239 T4164 T4171 arg1Of assay,was
R3240 T4164 T4172 arg2Of assay,performed
R3241 T4166 T4165 arg2Of antibodies,using
R3242 T4166 T4167 arg1Of antibodies,to
R3243 T4168 T4169 arg1Of P65,and
R3244 T4169 T4167 arg2Of and,to
R3245 T4170 T4169 arg2Of P50,and
R3246 T4172 T4171 arg2Of performed,was
R3247 T4172 T4173 modOf performed,to
R3248 T4174 T4173 arg1Of confirm,to
R3249 T4177 T4174 arg2Of specificity,confirm
R3250 T4177 T4175 arg1Of specificity,NFκB
R3251 T4177 T4176 arg1Of specificity,binding
R3252 T4177 T4178 arg1Of specificity,as
R3253 T4180 T4178 arg2Of described,as
R3254 T4180 T4179 arg1Of described,previously

bionlp-st-ge-2016-test-proteins

Id Subject Object Predicate Lexical cue
T4019 814-817 Protein denotes P50
T4018 806-809 Protein denotes P65

UBERON-AE

Id Subject Object Predicate Lexical cue
T4005 498-502 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tube
T4004 332-337 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tubes
T4003 224-229 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tubes
T4002 91-97 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
T4001 85-90 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain

GO-MF

Id Subject Object Predicate Lexical cue
T4023 792-802 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T4022 848-855 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T4021 628-635 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T4020 587-594 http://purl.obolibrary.org/obo/GO_0005488 denotes binding

GO-CC

Id Subject Object Predicate Lexical cue
T4025 792-802 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T4024 792-802 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies

sentences

Id Subject Object Predicate Lexical cue
T4015 766-892 Sentence denotes A super shift assay using antibodies to P65 and P50 was performed to confirm NFκB binding specificity as previously described.
T4014 582-765 Sentence denotes NFκB binding activity was examined in a 15 μL binding reaction mixture containing 15 μg of nuclear proteins and 35 fmol [γ-32P] labeled double-stranded NFκB consensus oligonucleotide.
T4013 534-581 Sentence denotes EMSA was performed as described previously [8].
T4012 424-533 Sentence denotes The supernatants (nuclear extract) were transferred to a clean prechilled tube and stored at −80°C until use.
T4011 328-423 Sentence denotes The tubes were centrifuged at maximum speed (~16,000 × g) in a micro centrifuge for 10 minutes.
T4010 220-327 Sentence denotes The tubes containing pellets were put on vortex for 15 seconds every 10 minutes, for a total of 40 minutes.
T4009 99-219 Sentence denotes Briefly, after removal of supernatant (cytoplasmic extract), the pellets were resuspended in nuclear extraction reagent.
T4008 49-98 Sentence denotes Nuclear proteins were isolated from brain tissue.
T4007 5-48 Sentence denotes Electrophoretic Mobility Shift Assay (EMSA)
T4006 0-4 Sentence denotes 2.6.
T84 0-4 Sentence denotes 2.6.
T85 5-48 Sentence denotes Electrophoretic Mobility Shift Assay (EMSA)
T86 49-98 Sentence denotes Nuclear proteins were isolated from brain tissue.
T87 99-219 Sentence denotes Briefly, after removal of supernatant (cytoplasmic extract), the pellets were resuspended in nuclear extraction reagent.
T88 220-327 Sentence denotes The tubes containing pellets were put on vortex for 15 seconds every 10 minutes, for a total of 40 minutes.
T89 328-423 Sentence denotes The tubes were centrifuged at maximum speed (~16,000 × g) in a micro centrifuge for 10 minutes.
T90 424-533 Sentence denotes The supernatants (nuclear extract) were transferred to a clean prechilled tube and stored at −80°C until use.
T91 534-581 Sentence denotes EMSA was performed as described previously [8].
T92 582-765 Sentence denotes NFκB binding activity was examined in a 15 μL binding reaction mixture containing 15 μg of nuclear proteins and 35 fmol [γ-32P] labeled double-stranded NFκB consensus oligonucleotide.
T93 766-892 Sentence denotes A super shift assay using antibodies to P65 and P50 was performed to confirm NFκB binding specificity as previously described.

simple1

Id Subject Object Predicate Lexical cue
T4033 814-817 Protein denotes P50
T4032 806-809 Protein denotes P65

BioNLP16_DUT

Id Subject Object Predicate Lexical cue
T4360 814-817 Protein denotes P50
T4359 806-809 Protein denotes P65

BioNLP16_Messiy

Id Subject Object Predicate Lexical cue
T4346 814-817 Protein denotes P50
T4345 806-809 Protein denotes P65

DLUT931

Id Subject Object Predicate Lexical cue
T4350 814-817 Protein denotes P50
T4349 806-809 Protein denotes P65

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T4377 814-817 Protein denotes P50
T4376 442-457 Entity denotes nuclear extract
T4375 328-337 Protein denotes The tubes
T4374 806-809 Protein denotes P65
T4373 718-738 Protein denotes double-stranded NFκB
T4372 220-248 Protein denotes The tubes containing pellets
T4371 636-663 Entity denotes reaction mixture containing
T4370 739-764 Entity denotes consensus oligonucleotide
T4369 673-689 Protein denotes nuclear proteins
T4368 424-458 Entity denotes The supernatants (nuclear extract)
T4367 192-218 Entity denotes nuclear extraction reagent
T4366 261-267 Entity denotes vortex
T4365 49-65 Protein denotes Nuclear proteins
T4364 122-158 Entity denotes of supernatant (cytoplasmic extract)
T4363 138-157 Entity denotes cytoplasmic extract
T4362 498-532 Protein denotes tube and stored at −80°C until use
T4361 792-802 Entity denotes antibodies

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T4344 891-892 . denotes .
T4343 882-891 VBN denotes described
T4342 871-881 RB denotes previously
T4341 868-870 IN denotes as
T4340 856-867 NN denotes specificity
T4339 848-855 JJ denotes binding
T4338 843-847 NNP denotes NFκB
T4337 835-842 VB denotes confirm
T4336 832-834 TO denotes to
T4335 822-831 VBN denotes performed
T4334 818-821 VBD denotes was
T4333 814-817 NNP denotes P50
T4332 810-813 CC denotes and
T4331 806-809 NNP denotes P65
T4330 803-805 TO denotes to
T4329 792-802 NNS denotes antibodies
T4328 786-791 VBG denotes using
T4327 780-785 NN denotes assay
T4326 774-779 NN denotes shift
T4325 768-773 JJ denotes super
T4324 766-767 DT denotes A
T4323 764-765 . denotes .
T4322 749-764 NN denotes oligonucleotide
T4321 739-748 NN denotes consensus
T4320 734-738 NNP denotes NFκB
T4319 718-733 JJ denotes double-stranded
T4318 710-717 VBD denotes labeled
T4317 708-709 NNP denotes ]
T4316 703-708 NNP denotes γ-32P
T4315 702-703 NNP denotes [
T4314 697-701 NN denotes fmol
T4313 694-696 CD denotes 35
T4312 690-693 CC denotes and
T4311 681-689 NNS denotes proteins
T4310 673-680 JJ denotes nuclear
T4309 670-672 IN denotes of
T4308 667-669 NN denotes μg
T4307 664-666 CD denotes 15
T4306 653-663 VBG denotes containing
T4305 645-652 NN denotes mixture
T4304 636-644 NN denotes reaction
T4303 628-635 JJ denotes binding
T4302 625-627 NN denotes μL
T4301 622-624 CD denotes 15
T4300 620-621 DT denotes a
T4299 617-619 IN denotes in
T4298 608-616 VBN denotes examined
T4297 604-607 VBD denotes was
T4296 595-603 NN denotes activity
T4295 587-594 JJ denotes binding
T4294 582-586 NNP denotes NFκB
T4293 580-581 . denotes .
T4292 579-580 NN denotes ]
T4291 578-579 CD denotes 8
T4290 577-578 JJ denotes [
T4289 566-576 RB denotes previously
T4288 556-565 VBN denotes described
T4287 553-555 IN denotes as
T4286 543-552 VBN denotes performed
T4285 539-542 VBD denotes was
T4284 534-538 NNP denotes EMSA
T4283 532-533 . denotes .
T4282 529-532 NN denotes use
T4281 523-528 IN denotes until
T4280 521-522 NNP denotes C
T4279 520-521 CD denotes °
T4278 518-520 CD denotes 80
T4277 517-518 CD denotes −
T4276 514-516 IN denotes at
T4275 507-513 VBD denotes stored
T4274 503-506 CC denotes and
T4273 498-502 NN denotes tube
T4272 487-497 JJ denotes prechilled
T4271 481-486 JJ denotes clean
T4270 479-480 DT denotes a
T4269 476-478 TO denotes to
T4268 464-475 VBN denotes transferred
T4267 459-463 VBD denotes were
T4266 457-458 -RRB- denotes )
T4265 450-457 VB denotes extract
T4264 442-449 JJ denotes nuclear
T4263 441-442 -LRB- denotes (
T4262 428-440 NNS denotes supernatants
T4261 424-427 DT denotes The
T4260 422-423 . denotes .
T4259 415-422 NNS denotes minutes
T4258 412-414 CD denotes 10
T4257 408-411 IN denotes for
T4256 397-407 NN denotes centrifuge
T4255 391-396 NN denotes micro
T4254 389-390 DT denotes a
T4253 386-388 IN denotes in
T4252 384-385 -RRB- denotes )
T4251 383-384 NN denotes g
T4250 381-382 CD denotes ×
T4249 374-380 CD denotes 16,000
T4248 373-374 FW denotes ~
T4247 372-373 -LRB- denotes (
T4246 366-371 NN denotes speed
T4245 358-365 JJ denotes maximum
T4244 355-357 IN denotes at
T4243 343-354 VBN denotes centrifuged
T4242 338-342 VBD denotes were
T4241 332-337 NNS denotes tubes
T4240 328-331 DT denotes The
T4218 220-223 DT denotes The
T4217 218-219 . denotes .
T4216 211-218 NN denotes reagent
T4215 200-210 NN denotes extraction
T4214 192-199 JJ denotes nuclear
T4213 189-191 IN denotes in
T4212 177-188 VBN denotes resuspended
T4211 172-176 VBD denotes were
T4210 164-171 NNS denotes pellets
T4209 160-163 DT denotes the
T4208 158-159 , denotes ,
T4207 157-158 -RRB- denotes )
T4206 150-157 VB denotes extract
T4205 138-149 JJ denotes cytoplasmic
T4204 137-138 -LRB- denotes (
T4203 125-136 JJ denotes supernatant
T4202 122-124 IN denotes of
T4201 114-121 NN denotes removal
T4200 108-113 IN denotes after
T4199 106-107 , denotes ,
T4198 99-106 RB denotes Briefly
T4197 97-98 . denotes .
T4196 91-97 NN denotes tissue
T4195 85-90 NN denotes brain
T4194 80-84 IN denotes from
T4193 71-79 VBN denotes isolated
T4192 66-70 VBD denotes were
T4191 57-65 NNS denotes proteins
T4190 49-56 NNP denotes Nuclear
T4189 47-48 -RRB- denotes )
T4188 43-47 NNP denotes EMSA
T4187 42-43 -LRB- denotes (
T4186 36-41 NNP denotes Assay
T4185 30-35 NNP denotes Shift
T4184 21-29 NNP denotes Mobility
T4183 5-20 NNP denotes Electrophoretic
T4182 3-4 . denotes .
T4181 0-3 CD denotes 2.6
T4239 326-327 . denotes .
T4238 319-326 NNS denotes minutes
T4237 316-318 CD denotes 40
T4236 313-315 IN denotes of
T4235 307-312 NN denotes total
T4234 305-306 DT denotes a
T4233 301-304 IN denotes for
T4232 299-300 , denotes ,
T4231 292-299 NNS denotes minutes
T4230 289-291 CD denotes 10
T4229 283-288 DT denotes every
T4228 275-282 NNS denotes seconds
T4227 272-274 CD denotes 15
T4226 268-271 IN denotes for
T4225 261-267 NN denotes vortex
T4224 258-260 IN denotes on
T4223 254-257 VBN denotes put
T4222 249-253 VBD denotes were
T4221 241-248 NNS denotes pellets
T4220 230-240 VBG denotes containing
T4219 224-229 NNS denotes tubes
R3278 T4204 T4206 punct (,extract
R3279 T4205 T4206 nsubj cytoplasmic,extract
R3280 T4206 T4206 ROOT extract,extract
R3281 T4207 T4206 punct ),extract
R3282 T4208 T4212 punct ",",resuspended
R3283 T4209 T4210 det the,pellets
R3284 T4210 T4212 nsubjpass pellets,resuspended
R3285 T4211 T4212 auxpass were,resuspended
R3286 T4212 T4212 ROOT resuspended,resuspended
R3287 T4213 T4212 prep in,resuspended
R3288 T4214 T4215 amod nuclear,extraction
R3289 T4215 T4213 pobj extraction,in
R3290 T4216 T4212 conj reagent,resuspended
R3291 T4217 T4212 punct .,resuspended
R3292 T4218 T4219 det The,tubes
R3293 T4219 T4223 nsubjpass tubes,put
R3294 T4220 T4219 acl containing,tubes
R3295 T4221 T4220 dobj pellets,containing
R3296 T4222 T4223 auxpass were,put
R3297 T4223 T4223 ROOT put,put
R3298 T4224 T4223 prep on,put
R3299 T4225 T4224 pobj vortex,on
R3300 T4226 T4223 prep for,put
R3301 T4227 T4228 nummod 15,seconds
R3302 T4228 T4226 pobj seconds,for
R3303 T4229 T4231 det every,minutes
R3304 T4230 T4231 nummod 10,minutes
R3305 T4231 T4223 npadvmod minutes,put
R3306 T4232 T4231 punct ",",minutes
R3307 T4233 T4231 prep for,minutes
R3308 T4234 T4235 det a,total
R3309 T4235 T4233 pobj total,for
R3310 T4236 T4235 prep of,total
R3311 T4237 T4238 nummod 40,minutes
R3312 T4238 T4236 pobj minutes,of
R3313 T4239 T4223 punct .,put
R3314 T4240 T4241 det The,tubes
R3315 T4241 T4243 nsubjpass tubes,centrifuged
R3316 T4242 T4243 auxpass were,centrifuged
R3317 T4243 T4243 ROOT centrifuged,centrifuged
R3318 T4244 T4243 prep at,centrifuged
R3319 T4245 T4246 amod maximum,speed
R3320 T4246 T4244 pobj speed,at
R3321 T4247 T4251 punct (,g
R3322 T4248 T4251 nmod ~,g
R3323 T4249 T4251 dep "16,000",g
R3324 T4250 T4251 nummod ×,g
R3325 T4251 T4246 appos g,speed
R3326 T4252 T4251 punct ),g
R3327 T4253 T4243 prep in,centrifuged
R3328 T4254 T4256 det a,centrifuge
R3329 T4255 T4256 amod micro,centrifuge
R3330 T4256 T4253 pobj centrifuge,in
R3331 T4257 T4243 prep for,centrifuged
R3332 T4258 T4259 nummod 10,minutes
R3333 T4259 T4257 pobj minutes,for
R3334 T4260 T4243 punct .,centrifuged
R3335 T4261 T4262 det The,supernatants
R3336 T4262 T4268 nsubjpass supernatants,transferred
R3337 T4263 T4262 punct (,supernatants
R3338 T4264 T4265 amod nuclear,extract
R3339 T4265 T4262 appos extract,supernatants
R3340 T4266 T4262 punct ),supernatants
R3341 T4267 T4268 auxpass were,transferred
R3342 T4268 T4268 ROOT transferred,transferred
R3343 T4269 T4268 prep to,transferred
R3344 T4270 T4273 det a,tube
R3345 T4271 T4273 amod clean,tube
R3346 T4272 T4273 amod prechilled,tube
R3347 T4273 T4269 pobj tube,to
R3348 T4274 T4268 cc and,transferred
R3349 T4275 T4268 conj stored,transferred
R3350 T4276 T4275 prep at,stored
R3351 T4277 T4279 nummod −,°
R3352 T4278 T4279 nummod 80,°
R3353 T4279 T4276 pobj °,at
R3354 T4280 T4275 npadvmod C,stored
R3355 T4281 T4275 prep until,stored
R3356 T4282 T4281 pobj use,until
R3357 T4283 T4268 punct .,transferred
R3358 T4284 T4286 nsubjpass EMSA,performed
R3359 T4285 T4286 auxpass was,performed
R3360 T4286 T4286 ROOT performed,performed
R3361 T4287 T4288 mark as,described
R3362 T4288 T4286 advcl described,performed
R3363 T4289 T4290 advmod previously,[
R3364 T4290 T4292 amod [,]
R3365 T4291 T4292 nummod 8,]
R3366 T4292 T4288 dobj ],described
R3367 T4293 T4286 punct .,performed
R3368 T4294 T4296 amod NFκB,activity
R3369 T4295 T4296 amod binding,activity
R3370 T4296 T4298 nsubjpass activity,examined
R3371 T4297 T4298 auxpass was,examined
R3372 T4298 T4298 ROOT examined,examined
R3373 T4299 T4298 prep in,examined
R3374 T4300 T4305 det a,mixture
R3375 T4301 T4302 nummod 15,μL
R3376 T4302 T4305 compound μL,mixture
R3377 T4303 T4304 amod binding,reaction
R3378 T4304 T4305 compound reaction,mixture
R3379 T4305 T4299 pobj mixture,in
R3380 T4306 T4305 acl containing,mixture
R3381 T4307 T4308 nummod 15,μg
R3382 T4308 T4317 nmod μg,]
R3383 T4309 T4308 prep of,μg
R3384 T4310 T4311 amod nuclear,proteins
R3385 T4311 T4309 pobj proteins,of
R3386 T4312 T4311 cc and,proteins
R3387 T4313 T4314 nummod 35,fmol
R3388 T4314 T4317 compound fmol,]
R3389 T4315 T4317 compound [,]
R3390 T4316 T4317 compound γ-32P,]
R3391 T4317 T4306 dobj ],containing
R3392 T4318 T4298 conj labeled,examined
R3393 T4319 T4322 amod double-stranded,oligonucleotide
R3394 T4320 T4321 compound NFκB,consensus
R3395 T4321 T4322 compound consensus,oligonucleotide
R3396 T4322 T4318 oprd oligonucleotide,labeled
R3397 T4323 T4298 punct .,examined
R3398 T4324 T4327 det A,assay
R3399 T4325 T4326 amod super,shift
R3400 T4326 T4327 compound shift,assay
R3401 T4327 T4335 nsubjpass assay,performed
R3402 T4328 T4327 acl using,assay
R3403 T4329 T4328 dobj antibodies,using
R3404 T4330 T4328 prep to,using
R3405 T4331 T4330 pobj P65,to
R3406 T4332 T4331 cc and,P65
R3407 T4333 T4331 conj P50,P65
R3408 T4334 T4335 auxpass was,performed
R3409 T4335 T4335 ROOT performed,performed
R3410 T4336 T4337 aux to,confirm
R3411 T4337 T4335 xcomp confirm,performed
R3412 T4338 T4340 nmod NFκB,specificity
R3413 T4339 T4340 amod binding,specificity
R3414 T4340 T4337 dobj specificity,confirm
R3415 T4341 T4343 mark as,described
R3416 T4342 T4343 advmod previously,described
R3417 T4343 T4340 advcl described,specificity
R3418 T4344 T4335 punct .,performed
R3255 T4181 T4181 ROOT 2.6,2.6
R3256 T4182 T4181 punct .,2.6
R3257 T4183 T4186 compound Electrophoretic,Assay
R3258 T4184 T4185 compound Mobility,Shift
R3259 T4185 T4186 compound Shift,Assay
R3260 T4186 T4193 nsubjpass Assay,isolated
R3261 T4187 T4188 punct (,EMSA
R3262 T4188 T4186 appos EMSA,Assay
R3263 T4189 T4188 punct ),EMSA
R3264 T4190 T4191 compound Nuclear,proteins
R3265 T4191 T4192 nsubj proteins,were
R3266 T4192 T4193 auxpass were,isolated
R3267 T4193 T4193 ROOT isolated,isolated
R3268 T4194 T4193 prep from,isolated
R3269 T4195 T4196 compound brain,tissue
R3270 T4196 T4194 pobj tissue,from
R3271 T4197 T4193 punct .,isolated
R3272 T4198 T4206 advmod Briefly,extract
R3273 T4199 T4206 punct ",",extract
R3274 T4200 T4206 prep after,extract
R3275 T4201 T4200 pobj removal,after
R3276 T4202 T4201 prep of,removal
R3277 T4203 T4202 pobj supernatant,of

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T4031 843-847 Protein denotes NFκB
T4030 814-817 Protein denotes P50
T4029 806-809 Protein denotes P65
T4028 587-594 Binding denotes binding
T4027 718-764 Protein denotes double-stranded NFκB consensus oligonucleotide
T4026 582-586 Protein denotes NFκB
R3108 T4026 T4028 themeOf NFκB,binding

testone

Id Subject Object Predicate Lexical cue
T3995 848-855 Binding denotes binding
T3994 814-817 Protein denotes P50
T3993 806-809 Protein denotes P65
R3104 T3993 T3995 themeOf P65,binding
R3105 T3994 T3995 themeOf P50,binding

test3

Id Subject Object Predicate Lexical cue
T4000 848-855 Binding denotes binding
T3999 814-817 Protein denotes P50
T3998 806-809 Protein denotes P65
T3997 814-817 Protein denotes P50
T3996 806-809 Protein denotes P65
R3106 T3998 T4000 themeOf P65,binding
R3107 T3999 T4000 themeOf P50,binding