PMC:2817507 / 7751-8455
Annnotations
pmc-enju-pas
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Western Blots\nCellular proteins were prepared from ICH cerebral hemispheres, electrophoresed with SDS-polyacrylamide gel and transferred onto Hybond ECL membranes (Amersham Pharmacia, Piscataway, NJ). The ECL membranes were incubated with the appropriate primary antibody (WuHan, BA1717) followed by incubation with peroxidase-conjugated secondary antibodies (Golden Bridge, PV-6001). The signals were detected with the ECL system (Amersham Pharmacia). The same membranes were probed with anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase, Biodesign, Saco, Maine) after being washed with stripping buffer. The signals were quantified by scanning densitometry and computer-assisted image analysis."}
bionlp-st-ge-2016-test-proteins
{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T3726","span":{"begin":506,"end":546},"obj":"Protein"},{"id":"T3725","span":{"begin":499,"end":504},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"2.5. Western Blots\nCellular proteins were prepared from ICH cerebral hemispheres, electrophoresed with SDS-polyacrylamide gel and transferred onto Hybond ECL membranes (Amersham Pharmacia, Piscataway, NJ). The ECL membranes were incubated with the appropriate primary antibody (WuHan, BA1717) followed by incubation with peroxidase-conjugated secondary antibodies (Golden Bridge, PV-6001). The signals were detected with the ECL system (Amersham Pharmacia). The same membranes were probed with anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase, Biodesign, Saco, Maine) after being washed with stripping buffer. The signals were quantified by scanning densitometry and computer-assisted image analysis."}
bionlp-st-ge-2016-uniprot
{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T3959","span":{"begin":521,"end":546},"obj":"http://www.uniprot.org/uniprot/P04406"},{"id":"T3958","span":{"begin":499,"end":504},"obj":"http://www.uniprot.org/uniprot/P04406"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"2.5. Western Blots\nCellular proteins were prepared from ICH cerebral hemispheres, electrophoresed with SDS-polyacrylamide gel and transferred onto Hybond ECL membranes (Amersham Pharmacia, Piscataway, NJ). The ECL membranes were incubated with the appropriate primary antibody (WuHan, BA1717) followed by incubation with peroxidase-conjugated secondary antibodies (Golden Bridge, PV-6001). The signals were detected with the ECL system (Amersham Pharmacia). The same membranes were probed with anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase, Biodesign, Saco, Maine) after being washed with stripping buffer. The signals were quantified by scanning densitometry and computer-assisted image analysis."}
UBERON-AE
{"project":"UBERON-AE","denotations":[{"id":"T3715","span":{"begin":60,"end":80},"obj":"http://purl.obolibrary.org/obo/UBERON_0001869"}],"text":"2.5. Western Blots\nCellular proteins were prepared from ICH cerebral hemispheres, electrophoresed with SDS-polyacrylamide gel and transferred onto Hybond ECL membranes (Amersham Pharmacia, Piscataway, NJ). The ECL membranes were incubated with the appropriate primary antibody (WuHan, BA1717) followed by incubation with peroxidase-conjugated secondary antibodies (Golden Bridge, PV-6001). The signals were detected with the ECL system (Amersham Pharmacia). The same membranes were probed with anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase, Biodesign, Saco, Maine) after being washed with stripping buffer. The signals were quantified by scanning densitometry and computer-assisted image analysis."}
GO-BP
{"project":"GO-BP","denotations":[{"id":"T3729","span":{"begin":618,"end":625},"obj":"http://purl.obolibrary.org/obo/GO_0023052"},{"id":"T3728","span":{"begin":394,"end":401},"obj":"http://purl.obolibrary.org/obo/GO_0023052"},{"id":"T3727","span":{"begin":19,"end":27},"obj":"http://purl.obolibrary.org/obo/GO_0007349"}],"text":"2.5. Western Blots\nCellular proteins were prepared from ICH cerebral hemispheres, electrophoresed with SDS-polyacrylamide gel and transferred onto Hybond ECL membranes (Amersham Pharmacia, Piscataway, NJ). The ECL membranes were incubated with the appropriate primary antibody (WuHan, BA1717) followed by incubation with peroxidase-conjugated secondary antibodies (Golden Bridge, PV-6001). The signals were detected with the ECL system (Amersham Pharmacia). The same membranes were probed with anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase, Biodesign, Saco, Maine) after being washed with stripping buffer. The signals were quantified by scanning densitometry and computer-assisted image analysis."}
GO-MF
{"project":"GO-MF","denotations":[{"id":"T3731","span":{"begin":353,"end":363},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T3730","span":{"begin":268,"end":276},"obj":"http://purl.obolibrary.org/obo/GO_0003823"}],"text":"2.5. Western Blots\nCellular proteins were prepared from ICH cerebral hemispheres, electrophoresed with SDS-polyacrylamide gel and transferred onto Hybond ECL membranes (Amersham Pharmacia, Piscataway, NJ). The ECL membranes were incubated with the appropriate primary antibody (WuHan, BA1717) followed by incubation with peroxidase-conjugated secondary antibodies (Golden Bridge, PV-6001). The signals were detected with the ECL system (Amersham Pharmacia). The same membranes were probed with anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase, Biodesign, Saco, Maine) after being washed with stripping buffer. The signals were quantified by scanning densitometry and computer-assisted image analysis."}
GO-CC
{"project":"GO-CC","denotations":[{"id":"T3738","span":{"begin":353,"end":363},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T3737","span":{"begin":268,"end":276},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T3736","span":{"begin":353,"end":363},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T3735","span":{"begin":268,"end":276},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T3734","span":{"begin":467,"end":476},"obj":"http://purl.obolibrary.org/obo/GO_0016020"},{"id":"T3733","span":{"begin":214,"end":223},"obj":"http://purl.obolibrary.org/obo/GO_0016020"},{"id":"T3732","span":{"begin":158,"end":167},"obj":"http://purl.obolibrary.org/obo/GO_0016020"}],"text":"2.5. Western Blots\nCellular proteins were prepared from ICH cerebral hemispheres, electrophoresed with SDS-polyacrylamide gel and transferred onto Hybond ECL membranes (Amersham Pharmacia, Piscataway, NJ). The ECL membranes were incubated with the appropriate primary antibody (WuHan, BA1717) followed by incubation with peroxidase-conjugated secondary antibodies (Golden Bridge, PV-6001). The signals were detected with the ECL system (Amersham Pharmacia). The same membranes were probed with anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase, Biodesign, Saco, Maine) after being washed with stripping buffer. The signals were quantified by scanning densitometry and computer-assisted image analysis."}
sentences
{"project":"sentences","denotations":[{"id":"T3722","span":{"begin":614,"end":704},"obj":"Sentence"},{"id":"T3721","span":{"begin":458,"end":613},"obj":"Sentence"},{"id":"T3720","span":{"begin":390,"end":457},"obj":"Sentence"},{"id":"T3719","span":{"begin":206,"end":389},"obj":"Sentence"},{"id":"T3718","span":{"begin":19,"end":205},"obj":"Sentence"},{"id":"T3717","span":{"begin":5,"end":18},"obj":"Sentence"},{"id":"T3716","span":{"begin":0,"end":4},"obj":"Sentence"},{"id":"T77","span":{"begin":0,"end":4},"obj":"Sentence"},{"id":"T78","span":{"begin":5,"end":18},"obj":"Sentence"},{"id":"T79","span":{"begin":19,"end":205},"obj":"Sentence"},{"id":"T80","span":{"begin":206,"end":389},"obj":"Sentence"},{"id":"T81","span":{"begin":390,"end":457},"obj":"Sentence"},{"id":"T82","span":{"begin":458,"end":613},"obj":"Sentence"},{"id":"T83","span":{"begin":614,"end":704},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"2.5. Western Blots\nCellular proteins were prepared from ICH cerebral hemispheres, electrophoresed with SDS-polyacrylamide gel and transferred onto Hybond ECL membranes (Amersham Pharmacia, Piscataway, NJ). The ECL membranes were incubated with the appropriate primary antibody (WuHan, BA1717) followed by incubation with peroxidase-conjugated secondary antibodies (Golden Bridge, PV-6001). The signals were detected with the ECL system (Amersham Pharmacia). The same membranes were probed with anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase, Biodesign, Saco, Maine) after being washed with stripping buffer. The signals were quantified by scanning densitometry and computer-assisted image analysis."}
simple1
{"project":"simple1","denotations":[{"id":"T3743","span":{"begin":506,"end":546},"obj":"Protein"},{"id":"T3742","span":{"begin":499,"end":504},"obj":"Protein"}],"text":"2.5. Western Blots\nCellular proteins were prepared from ICH cerebral hemispheres, electrophoresed with SDS-polyacrylamide gel and transferred onto Hybond ECL membranes (Amersham Pharmacia, Piscataway, NJ). The ECL membranes were incubated with the appropriate primary antibody (WuHan, BA1717) followed by incubation with peroxidase-conjugated secondary antibodies (Golden Bridge, PV-6001). The signals were detected with the ECL system (Amersham Pharmacia). The same membranes were probed with anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase, Biodesign, Saco, Maine) after being washed with stripping buffer. The signals were quantified by scanning densitometry and computer-assisted image analysis."}
BioNLP16_DUT
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BioNLP16_Messiy
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DLUT931
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bionlp-st-ge-2016-test-ihmc
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bionlp-st-ge-2016-spacy-parsed
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bionlp-st-ge-2016-test-tees
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testone
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test3
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