PMC:2817507 / 3530-9582 JSON TXT

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2_test

Id	Subject	Object	Predicate	Lexical cue
20150961-17884182-67933705	965-966	17884182	denotes	8
20150961-17884182-67933706	3901-3902	17884182	denotes	8
20150961-17884182-67933707	5505-5506	17884182	denotes	8

pmc-enju-pas

Id	Subject	Object	Predicate	Lexical cue
T4425	6039-6051	NN	denotes	significance
T4424	6036-6038	IN	denotes	of
T4423	6027-6035	NN	denotes	criteria
T4422	6023-6026	DT	denotes	the
T4421	6020-6022	IN	denotes	as
T4420	6015-6019	VB	denotes	used
T4419	6011-6014	VB	denotes	was
T4418	5999-6010	NN	denotes	probability
T4417	5996-5998	IN	denotes	of
T4416	5990-5995	NN	denotes	level
T4415	5986-5989	CD	denotes	.05
T4414	5984-5985	JJ	denotes	<
T4413	5982-5983	NN	denotes	P
T4412	5978-5981	DT	denotes	The
T4411	5970-5976	NN	denotes	t-test
T4410	5967-5969	POS	denotes	's
T4409	5960-5967	NN	denotes	Student
T4408	5957-5959	CC	denotes	or
T4407	5955-5956	-RRB-	denotes	)
T4406	5950-5955	NN	denotes	ANOVA
T4405	5949-5950	-LRB-	denotes	(
T4404	5940-5948	NN	denotes	variance
T4403	5937-5939	IN	denotes	of
T4402	5928-5936	NN	denotes	analysis
T4401	5920-5927	JJ	denotes	one-way
T4399	5908-5916	VB	denotes	analyzed
T4398	5903-5907	VB	denotes	were
T4397	5890-5902	NN	denotes	measurements
T4396	5886-5889	DT	denotes	The
T4395	5881-5884	NN	denotes	SEM
T4394	5879-5880	NN	denotes	±
T4393	5874-5878	JJ	denotes	mean
T4392	5871-5873	IN	denotes	as
T4391	5861-5870	VB	denotes	presented
T4390	5856-5860	VB	denotes	were
T4389	5851-5855	NN	denotes	data
T4388	5847-5850	DT	denotes	All
T4387	5838-5846	NN	denotes	Analysis
T4386	5826-5837	JJ	denotes	Statistical
T4385	5821-5824	CD	denotes	2.7
T4046	5007-5011	IN	denotes	from
T4045	4998-5006	VB	denotes	isolated
T4044	4993-4997	VB	denotes	were
T4043	4984-4992	NN	denotes	proteins
T4042	4976-4983	JJ	denotes	Nuclear
T4041	4974-4975	-RRB-	denotes	)
T4040	4970-4974	NN	denotes	EMSA
T4039	4969-4970	-LRB-	denotes	(
T4038	4963-4968	NN	denotes	Assay
T4037	4957-4962	NN	denotes	Shift
T4036	4948-4956	NN	denotes	Mobility
T4035	4932-4947	JJ	denotes	Electrophoretic
T4034	4927-4930	CD	denotes	2.6
T4400	5917-5919	IN	denotes	by
T3847	4916-4924	NN	denotes	analysis
T3846	4910-4915	NN	denotes	image
T3845	4892-4909	JJ	denotes	computer-assisted
T3844	4888-4891	CC	denotes	and
T3843	4875-4887	NN	denotes	densitometry
T3842	4866-4874	VB	denotes	scanning
T3841	4863-4865	IN	denotes	by
T3840	4852-4862	VB	denotes	quantified
T3839	4847-4851	VB	denotes	were
T3838	4839-4846	NN	denotes	signals
T3837	4835-4838	DT	denotes	The
T3836	4827-4833	NN	denotes	buffer
T3835	4817-4826	VB	denotes	stripping
T3834	4812-4816	IN	denotes	with
T3833	4805-4811	VB	denotes	washed
T3832	4799-4804	VB	denotes	being
T3831	4793-4798	IN	denotes	after
T3790	4523-4525	IN	denotes	by
T3789	4514-4522	VB	denotes	followed
T3788	4512-4513	-RRB-	denotes	)
T3787	4506-4512	NN	denotes	BA1717
T3786	4504-4505	-COMMA-	denotes	,
T3785	4499-4504	NN	denotes	WuHan
T3784	4498-4499	-LRB-	denotes	(
T3783	4489-4497	NN	denotes	antibody
T3782	4481-4488	JJ	denotes	primary
T3781	4469-4480	JJ	denotes	appropriate
T3780	4465-4468	DT	denotes	the
T3779	4460-4464	IN	denotes	with
T3778	4450-4459	VB	denotes	incubated
T3777	4445-4449	VB	denotes	were
T3776	4435-4444	NN	denotes	membranes
T3775	4431-4434	NN	denotes	ECL
T3774	4427-4430	DT	denotes	The
T3773	4424-4425	-RRB-	denotes	)
T3772	4422-4424	NNP	denotes	NJ
T3771	4420-4421	-COMMA-	denotes	,
T3770	4410-4420	NNP	denotes	Piscataway
T3769	4408-4409	-COMMA-	denotes	,
T3768	4399-4408	NNP	denotes	Pharmacia
T3767	4390-4398	NNP	denotes	Amersham
T3766	4389-4390	-LRB-	denotes	(
T3765	4379-4388	NN	denotes	membranes
T3764	4375-4378	NN	denotes	ECL
T3763	4368-4374	NNP	denotes	Hybond
T3762	4363-4367	IN	denotes	onto
T3761	4351-4362	VB	denotes	transferred
T3760	4347-4350	CC	denotes	and
T3759	4343-4346	NN	denotes	gel
T3758	4324-4342	NN	denotes	SDS-polyacrylamide
T3757	4319-4323	IN	denotes	with
T3756	4303-4318	VB	denotes	electrophoresed
T3755	4301-4302	-COMMA-	denotes	,
T3754	4290-4301	NN	denotes	hemispheres
T3753	4281-4289	JJ	denotes	cerebral
T3752	4277-4280	NN	denotes	ICH
T3751	4272-4276	IN	denotes	from
T3750	4263-4271	VB	denotes	prepared
T3749	4258-4262	VB	denotes	were
T3748	4249-4257	NN	denotes	proteins
T3747	4240-4248	JJ	denotes	Cellular
T3746	4234-4239	NN	denotes	Blots
T3745	4226-4233	JJ	denotes	Western
T3744	4221-4224	CD	denotes	2.5
T4180	5809-5818	VB	denotes	described
T4179	5798-5808	RB	denotes	previously
T4178	5795-5797	IN	denotes	as
T4177	5783-5794	NN	denotes	specificity
T4176	5775-5782	NN	denotes	binding
T4175	5770-5774	NN	denotes	NFκB
T4174	5762-5769	VB	denotes	confirm
T4173	5759-5761	TO	denotes	to
T4172	5749-5758	VB	denotes	performed
T4171	5745-5748	VB	denotes	was
T4170	5741-5744	NN	denotes	P50
T4169	5737-5740	CC	denotes	and
T4168	5733-5736	NN	denotes	P65
T4167	5730-5732	TO	denotes	to
T4166	5719-5729	NN	denotes	antibodies
T4165	5713-5718	VB	denotes	using
T4164	5707-5712	NN	denotes	assay
T4163	5701-5706	NN	denotes	shift
T4162	5695-5700	JJ	denotes	super
T4161	5693-5694	DT	denotes	A
T4160	5676-5691	NN	denotes	oligonucleotide
T4159	5666-5675	NN	denotes	consensus
T4158	5661-5665	NN	denotes	NFκB
T4157	5645-5660	JJ	denotes	double-stranded
T4156	5637-5644	VB	denotes	labeled
T4155	5635-5636	-RRB-	denotes	]
T4154	5630-5635	NN	denotes	γ-32P
T4153	5629-5630	-LRB-	denotes	[
T4152	5621-5628	NN	denotes	35 fmol
T4151	5617-5620	CC	denotes	and
T4150	5608-5616	NN	denotes	proteins
T4149	5600-5607	JJ	denotes	nuclear
T4148	5597-5599	IN	denotes	of
T4147	5591-5596	NN	denotes	15 μg
T4146	5580-5590	VB	denotes	containing
T4145	5572-5579	NN	denotes	mixture
T4144	5563-5571	NN	denotes	reaction
T4143	5555-5562	NN	denotes	binding
T4142	5549-5554	NN	denotes	15 μL
T4141	5547-5548	DT	denotes	a
T4140	5544-5546	IN	denotes	in
T4139	5535-5543	VB	denotes	examined
T4138	5531-5534	VB	denotes	was
T4137	5522-5530	NN	denotes	activity
T4136	5514-5521	NN	denotes	binding
T4135	5509-5513	NN	denotes	NFκB
T4134	5506-5507	-RRB-	denotes	]
T4133	5505-5506	CD	denotes	8
T4132	5504-5505	-LRB-	denotes	[
T4131	5493-5503	RB	denotes	previously
T4130	5483-5492	VB	denotes	described
T4129	5480-5482	IN	denotes	as
T4128	5470-5479	VB	denotes	performed
T4127	5466-5469	VB	denotes	was
T4126	5461-5465	NN	denotes	EMSA
T4125	5456-5459	NN	denotes	use
T4124	5450-5455	IN	denotes	until
T4123	5444-5449	NN	denotes	−80°C
T4122	5441-5443	IN	denotes	at
T4121	5434-5440	VB	denotes	stored
T4120	5430-5433	CC	denotes	and
T4119	5425-5429	NN	denotes	tube
T4118	5414-5424	JJ	denotes	prechilled
T4117	5408-5413	JJ	denotes	clean
T4116	5406-5407	DT	denotes	a
T4115	5403-5405	TO	denotes	to
T4114	5391-5402	VB	denotes	transferred
T4113	5386-5390	VB	denotes	were
T4112	5384-5385	-RRB-	denotes	)
T4111	5377-5384	NN	denotes	extract
T4110	5369-5376	JJ	denotes	nuclear
T4109	5368-5369	-LRB-	denotes	(
T4108	5355-5367	NN	denotes	supernatants
T4107	5351-5354	DT	denotes	The
T4106	5342-5349	NN	denotes	minutes
T4105	5339-5341	CD	denotes	10
T4104	5335-5338	IN	denotes	for
T4103	5324-5334	NN	denotes	centrifuge
T4102	5318-5323	NN	denotes	micro
T4101	5316-5317	DT	denotes	a
T4100	5313-5315	IN	denotes	in
T4099	5311-5312	-RRB-	denotes	)
T4098	5308-5311	NN	denotes	× g
T4097	5300-5307	CD	denotes	~16,000
T4096	5299-5300	-LRB-	denotes	(
T4095	5293-5298	NN	denotes	speed
T4094	5285-5292	JJ	denotes	maximum
T4093	5282-5284	IN	denotes	at
T4092	5270-5281	VB	denotes	centrifuged
T4091	5265-5269	VB	denotes	were
T4090	5259-5264	NN	denotes	tubes
T4089	5255-5258	DT	denotes	The
T4088	5246-5253	NN	denotes	minutes
T4087	5243-5245	CD	denotes	40
T4086	5240-5242	IN	denotes	of
T4085	5234-5239	NN	denotes	total
T4084	5232-5233	DT	denotes	a
T4083	5228-5231	IN	denotes	for
T4082	5226-5227	-COMMA-	denotes	,
T4081	5219-5226	NN	denotes	minutes
T4080	5216-5218	CD	denotes	10
T4079	5210-5215	DT	denotes	every
T4078	5202-5209	NN	denotes	seconds
T4077	5199-5201	CD	denotes	15
T4076	5195-5198	IN	denotes	for
T4075	5188-5194	NN	denotes	vortex
T4074	5185-5187	IN	denotes	on
T4073	5181-5184	VB	denotes	put
T4072	5176-5180	VB	denotes	were
T4071	5168-5175	NN	denotes	pellets
T4070	5157-5167	VB	denotes	containing
T4069	5151-5156	NN	denotes	tubes
T4068	5147-5150	DT	denotes	The
T4067	5138-5145	NN	denotes	reagent
T4066	5127-5137	NN	denotes	extraction
T4065	5119-5126	JJ	denotes	nuclear
T4064	5116-5118	IN	denotes	in
T4063	5104-5115	VB	denotes	resuspended
T4062	5099-5103	VB	denotes	were
T4061	5091-5098	NN	denotes	pellets
T4060	5087-5090	DT	denotes	the
T4059	5085-5086	-COMMA-	denotes	,
T4058	5084-5085	-RRB-	denotes	)
T4057	5077-5084	NN	denotes	extract
T4056	5065-5076	JJ	denotes	cytoplasmic
T4055	5064-5065	-LRB-	denotes	(
T4054	5052-5063	NN	denotes	supernatant
T4053	5049-5051	IN	denotes	of
T4052	5041-5048	NN	denotes	removal
T4051	5035-5040	IN	denotes	after
T4050	5033-5034	-COMMA-	denotes	,
T4049	5026-5033	RB	denotes	Briefly
T4048	5018-5024	NN	denotes	tissue
T4047	5012-5017	NN	denotes	brain
T3605	4210-4218	NN	denotes	controls
T3604	4201-4209	JJ	denotes	negative
T3603	4198-4200	IN	denotes	as
T3602	4191-4197	VB	denotes	served
T3601	4180-4190	NN	denotes	antibodies
T3600	4172-4179	JJ	denotes	primary
T3599	4164-4171	IN	denotes	without
T3598	4154-4163	VB	denotes	processed
T3597	4147-4153	NN	denotes	Slides
T3596	4144-4145	-RRB-	denotes	)
T3595	4142-4144	NNP	denotes	CA
T3594	4140-4141	-COMMA-	denotes	,
T3593	4136-4140	NNP	denotes	Cruz
T3592	4130-4135	NNP	denotes	Santa
T3591	4129-4130	-LRB-	denotes	(
T3590	4124-4128	NNP	denotes	Inc.
T3589	4110-4123	NNP	denotes	Biotechnology
T3588	4105-4109	NNP	denotes	Cruz
T3587	4099-4104	NNP	denotes	Santa
T3586	4094-4098	IN	denotes	from
T3585	4085-4093	VB	denotes	obtained
T3584	4080-4084	VB	denotes	were
T3583	4078-4079	-RRB-	denotes	)
T3582	4071-4078	NN	denotes	SC-2018
T3581	4069-4070	-COMMA-	denotes	,
T3580	4062-4069	NN	denotes	reagent
T3579	4058-4061	NN	denotes	ABC
T3578	4057-4058	-LRB-	denotes	(
T3577	4047-4056	NN	denotes	complexes
T3576	4013-4046	JJ	denotes	antibody-biotin-avidin-peroxidase
T3575	4009-4012	CC	denotes	and
T3830	4791-4792	-RRB-	denotes	)
T3829	4786-4791	NNP	denotes	Maine
T3828	4784-4785	-COMMA-	denotes	,
T3827	4780-4784	NNP	denotes	Saco
T3826	4778-4779	-COMMA-	denotes	,
T3825	4769-4778	NNP	denotes	Biodesign
T3824	4767-4768	-COMMA-	denotes	,
T3823	4754-4767	NN	denotes	dehydrogenase
T3822	4727-4753	NN	denotes	glyceraldehyde-3-phosphate
T3821	4726-4727	-LRB-	denotes	(
T3820	4715-4725	NN	denotes	anti-GAPDH
T3819	4710-4714	IN	denotes	with
T3818	4703-4709	VB	denotes	probed
T3817	4698-4702	VB	denotes	were
T3816	4688-4697	NN	denotes	membranes
T3815	4683-4687	JJ	denotes	same
T3814	4679-4682	DT	denotes	The
T3813	4676-4677	-RRB-	denotes	)
T3812	4667-4676	NNP	denotes	Pharmacia
T3811	4658-4666	NNP	denotes	Amersham
T3810	4657-4658	-LRB-	denotes	(
T3809	4650-4656	NN	denotes	system
T3808	4646-4649	NN	denotes	ECL
T3807	4642-4645	DT	denotes	the
T3806	4637-4641	IN	denotes	with
T3805	4628-4636	VB	denotes	detected
T3804	4623-4627	VB	denotes	were
T3803	4615-4622	NN	denotes	signals
T3802	4611-4614	DT	denotes	The
T3801	4608-4609	-RRB-	denotes	)
T3800	4601-4608	NN	denotes	PV-6001
T3799	4599-4600	-COMMA-	denotes	,
T3798	4593-4599	NNP	denotes	Bridge
T3797	4586-4592	NNP	denotes	Golden
T3796	4585-4586	-LRB-	denotes	(
T3795	4574-4584	NN	denotes	antibodies
T3794	4564-4573	JJ	denotes	secondary
T3793	4542-4563	JJ	denotes	peroxidase-conjugated
T3792	4537-4541	IN	denotes	with
T3791	4526-4536	NN	denotes	incubation
T3068	3797-3801	NN	denotes	mRNA
T3067	3789-3796	NN	denotes	β-actin
T3066	3786-3788	TO	denotes	to
T3065	3781-3785	NN	denotes	mRNA
T3064	3776-3780	NN	denotes	TLR4
T3063	3773-3775	IN	denotes	of
T3062	3767-3772	NN	denotes	ratio
T3061	3763-3766	DT	denotes	the
T3060	3760-3762	IN	denotes	as
T3059	3750-3759	VB	denotes	expressed
T3058	3746-3749	VB	denotes	was
T3057	3741-3745	NN	denotes	mRNA
T3056	3736-3740	NN	denotes	TLR4
T3055	3733-3735	IN	denotes	of
T3054	3723-3732	NN	denotes	abundance
T3053	3719-3722	DT	denotes	The
T3052	3708-3717	NN	denotes	densities
T3051	3703-3707	NN	denotes	band
T3050	3693-3702	VB	denotes	determine
T3049	3690-3692	TO	denotes	to
T3048	3685-3689	VB	denotes	used
T3047	3680-3684	VB	denotes	were
T3046	3671-3679	NN	denotes	software
T3045	3662-3670	NN	denotes	Analysis
T3044	3658-3661	CC	denotes	and
T3043	3646-3657	NNP	denotes	Acquisition
T3042	3640-3645	NN	denotes	Image
T3041	3635-3638	NN	denotes	gel
T3040	3627-3634	NN	denotes	agarose
T3039	3619-3626	IN	denotes	through
T3038	3603-3618	VB	denotes	electrophoresed
T3037	3598-3602	VB	denotes	were
T3036	3589-3597	NN	denotes	products
T3035	3585-3588	NN	denotes	PCR
T3034	3577-3583	NN	denotes	cycles
T3033	3574-3576	CD	denotes	31
T3032	3568-3573	IN	denotes	after
T3031	3560-3567	VB	denotes	stopped
T3030	3556-3559	VB	denotes	was
T3029	3547-3555	NN	denotes	reaction
T3028	3543-3546	DT	denotes	The
T3027	3537-3541	NN	denotes	60 s
T3026	3532-3536	NN	denotes	72°C
T3025	3528-3531	CC	denotes	and
T3024	3526-3527	-COMMA-	denotes	,
T3023	3522-3526	NN	denotes	40 s
T3022	3517-3521	NN	denotes	55°C
T3021	3515-3516	-COMMA-	denotes	,
T3020	3511-3515	NN	denotes	40 s
T3019	3506-3510	NN	denotes	94°C
T3018	3504-3505	-COMMA-	denotes	,
T3017	3499-3504	NN	denotes	2 min
T3016	3494-3498	NN	denotes	94°C
T3015	3492-3493	-COLON-	denotes	:
T3014	3483-3492	NN	denotes	condition
T3013	3473-3482	VB	denotes	following
T3012	3469-3472	DT	denotes	the
T3011	3466-3468	IN	denotes	at
T3010	3456-3465	VB	denotes	performed
T3009	3451-3455	VB	denotes	were
T3008	3444-3450	NN	denotes	cycles
T3007	3435-3443	NN	denotes	Reaction
T3006	3408-3433	NN	denotes	5′-CCAGGATAGAGCCACCAAT-3′
T3005	3406-3407	-COLON-	denotes	:
T3004	3399-3406	NN	denotes	reverse
T3003	3397-3398	-COLON-	denotes	;
T3002	3372-3397	NN	denotes	5′-GCCAACCGTGAAAAAGATG-3′
T3001	3370-3371	-COLON-	denotes	:
T3000	3363-3370	RB	denotes	forward
T2999	3361-3362	-COLON-	denotes	:
T2998	3360-3361	-RRB-	denotes	)
T2997	3354-3360	NN	denotes	701 bp
T2996	3353-3354	-LRB-	denotes	(
T2995	3345-3352	NN	denotes	β-actin
T2994	3343-3344	-COLON-	denotes	;
T2993	3317-3343	NN	denotes	5′-CAGGATGACACCATTGAAGC-3′
T2992	3315-3316	-COLON-	denotes	:
T2991	3308-3315	NN	denotes	reverse
T2990	3306-3307	-COLON-	denotes	;
T2989	3280-3306	NN	denotes	5′-AGCTTTGGTCAGTTGGCTCT-3′
T2988	3278-3279	-COLON-	denotes	:
T2987	3271-3278	RB	denotes	forward
T2986	3269-3270	-COLON-	denotes	:
T2985	3268-3269	-RRB-	denotes	)
T2984	3262-3268	NN	denotes	145 bp
T2983	3261-3262	-LRB-	denotes	(
T2982	3256-3260	NN	denotes	TLR4
T2981	3247-3254	NNP	denotes	GenBank
T2980	3244-3246	IN	denotes	in
T2979	3235-3243	VB	denotes	reported
T2978	3225-3234	NN	denotes	sequences
T2977	3220-3224	NN	denotes	gene
T2976	3212-3219	JJ	denotes	β-actin
T2975	3208-3211	CC	denotes	and
T2974	3203-3207	NN	denotes	TLR4
T2973	3199-3202	DT	denotes	the
T2972	3196-3198	TO	denotes	to
T2971	3186-3195	VB	denotes	according
T2970	3177-3185	VB	denotes	designed
T2969	3172-3176	VB	denotes	were
T2968	3162-3171	NN	denotes	sequences
T2967	3155-3161	NN	denotes	primer
T2966	3151-3154	NN	denotes	PCR
T2965	3147-3150	DT	denotes	The
T2964	3138-3145	NN	denotes	control
T2963	3129-3137	JJ	denotes	internal
T2962	3126-3128	DT	denotes	an
T2961	3123-3125	IN	denotes	as
T2960	3118-3122	VB	denotes	used
T2959	3114-3117	VB	denotes	was
T2958	3112-3113	-RRB-	denotes	)
T2957	3105-3112	NN	denotes	β-actin
T2956	3104-3105	-LRB-	denotes	(
T2955	3099-3103	NN	denotes	gene
T2954	3094-3098	NN	denotes	keep
T2953	3088-3093	NN	denotes	house
T2952	3084-3087	DT	denotes	The
T2951	3072-3082	NN	denotes	polymerase
T2950	3068-3071	NN	denotes	DNA
T2949	3064-3067	NN	denotes	Taq
T2948	3061-3063	NN	denotes	2u
T2947	3057-3060	CC	denotes	and
T2946	3055-3056	-COMMA-	denotes	,
T2945	3048-3055	NN	denotes	primers
T2944	3045-3047	IN	denotes	of
T2943	3038-3044	NN	denotes	200 μM
T2942	3036-3037	-COMMA-	denotes	,
T2941	3032-3036	NN	denotes	dNTP
T2940	3027-3031	DT	denotes	each
T2939	3024-3026	IN	denotes	of
T2938	3017-3023	NN	denotes	200 μM
T2937	3015-3016	-COMMA-	denotes	,
T2936	3003-3015	NN	denotes	1.5 mM MgCl2
T2935	3001-3002	-COMMA-	denotes	,
T2934	2995-3001	NN	denotes	buffer
T2933	2991-2994	NN	denotes	PCR
T2932	2988-2990	NN	denotes	1×
T2931	2983-2987	IN	denotes	with
T2930	2974-2982	NN	denotes	fragment
T2929	2969-2973	NN	denotes	gene
T2928	2964-2968	NN	denotes	TLR4
T2927	2960-2963	DT	denotes	the
T2926	2952-2959	VB	denotes	amplify
T2925	2949-2951	TO	denotes	to
T3574	4000-4008	NN	denotes	antibody
T3573	3990-3999	JJ	denotes	secondary
T3572	3977-3989	VB	denotes	biotinylated
T3571	3973-3976	DT	denotes	The
T3570	3970-3971	-RRB-	denotes	)
T3569	3964-3970	NN	denotes	BA1717
T3568	3962-3963	-COMMA-	denotes	,
T3567	3957-3962	NNP	denotes	WuHan
T3566	3956-3957	-LRB-	denotes	(
T3565	3946-3955	NN	denotes	anti-TLR4
T3564	3939-3945	NN	denotes	rabbit
T3563	3935-3938	VB	denotes	was
T3562	3926-3934	VB	denotes	employed
T3561	3917-3925	NN	denotes	antibody
T3560	3909-3916	JJ	denotes	primary
T3559	3905-3908	DT	denotes	The
T3558	3902-3903	-RRB-	denotes	]
T3557	3901-3902	CD	denotes	8
T3556	3900-3901	-LRB-	denotes	[
T3555	3889-3899	RB	denotes	previously
T3554	3879-3888	VB	denotes	described
T3553	3876-3878	IN	denotes	as
T3552	3866-3875	VB	denotes	performed
T3551	3862-3865	VB	denotes	was
T3550	3857-3861	NN	denotes	TLR4
T3549	3853-3856	IN	denotes	for
T3548	3844-3852	NN	denotes	staining
T3547	3840-3843	NN	denotes	IHC
T3546	3836-3839	DT	denotes	The
T3545	3834-3835	-RRB-	denotes	)
T3544	3831-3834	NN	denotes	IHC
T3543	3830-3831	-LRB-	denotes	(
T3542	3809-3829	NN	denotes	Immunohistochemistry
T3541	3804-3807	CD	denotes	2.4
T2924	2944-2948	VB	denotes	used
T2923	2940-2943	VB	denotes	was
T2922	2935-2939	NN	denotes	cDNA
T2921	2932-2934	IN	denotes	of
T2920	2921-2931	NN	denotes	microliter
T2919	2917-2920	CD	denotes	One
T2918	2907-2915	NN	denotes	supplier
T2917	2903-2906	DT	denotes	the
T2916	2900-2902	IN	denotes	by
T2915	2890-2899	VB	denotes	described
T2914	2879-2889	NN	denotes	conditions
T2913	2873-2878	IN	denotes	under
T2912	2871-2872	-RRB-	denotes	)
T2911	2868-2871	NNP	denotes	Inc
T2910	2860-2867	JJ	denotes	promega
T2909	2859-2860	-LRB-	denotes	(
T2908	2852-2858	NN	denotes	RT-PCR
T2907	2848-2851	IN	denotes	for
T2906	2844-2847	NN	denotes	Kit
T2905	2834-2843	NNP	denotes	Synthesis
T2904	2821-2833	NNP	denotes	First-Strand
T2903	2809-2820	NNP	denotes	Superscript
T2902	2803-2808	VB	denotes	using
T2901	2796-2802	NN	denotes	system
T2900	2787-2795	NN	denotes	reaction
T2899	2781-2786	NN	denotes	20 μL
T2898	2778-2780	IN	denotes	in
T2897	2773-2777	NN	denotes	cDNA
T2896	2768-2772	IN	denotes	into
T2895	2748-2767	VB	denotes	reverse-transcribed
T2894	2744-2747	VB	denotes	was
T2893	2740-2743	NN	denotes	RNA
T2892	2730-2738	NN	denotes	1.0 g/mL
T2891	2727-2729	TO	denotes	to
T2890	2718-2726	VB	denotes	adjusted
T2889	2713-2717	VB	denotes	were
T2888	2708-2712	NN	denotes	mRNA
T2887	2705-2707	IN	denotes	of
T2886	2690-2704	NN	denotes	concentrations
T2885	2686-2689	DT	denotes	the
T2884	2684-2685	-COMMA-	denotes	,
T2883	2680-2684	RB	denotes	Then
T2882	2668-2678	NN	denotes	wavelength
T2881	2660-2667	NN	denotes	A280 nm
T2880	2656-2659	CC	denotes	and
T2879	2651-2655	NN	denotes	A260
T2878	2648-2650	IN	denotes	at
T2877	2640-2647	NN	denotes	density
T2876	2632-2639	JJ	denotes	optical
T2875	2622-2631	VB	denotes	measuring
T2874	2619-2621	IN	denotes	by
T2873	2617-2618	-RRB-	denotes	)
T2448	2275-2283	VB	denotes	injected
T2447	2271-2274	VB	denotes	was
T2446	2264-2270	NN	denotes	saline
T2445	2257-2263	JJ	denotes	normal
T2444	2251-2256	NN	denotes	75 μL
T2443	2246-2250	RB	denotes	only
T2442	2244-2245	-COMMA-	denotes	,
T2441	2240-2244	NN	denotes	rats
T2440	2232-2239	NN	denotes	control
T2439	2225-2231	NN	denotes	saline
T2438	2218-2224	JJ	denotes	normal
T2437	2214-2217	DT	denotes	the
T2436	2210-2213	IN	denotes	For
T2435	2201-2208	NN	denotes	sutures
T2434	2196-2200	IN	denotes	with
T2433	2189-2195	VB	denotes	closed
T2432	2185-2188	VB	denotes	was
T2431	2176-2184	NN	denotes	incision
T2430	2171-2175	NN	denotes	skin
T2429	2167-2170	DT	denotes	the
T2428	2163-2166	CC	denotes	and
T2427	2161-2162	-COMMA-	denotes	,
T2426	2157-2161	NN	denotes	hole
T2425	2152-2156	NN	denotes	burr
T2424	2148-2151	DT	denotes	the
T2423	2141-2147	IN	denotes	around
T2422	2134-2140	VB	denotes	placed
T2421	2130-2133	VB	denotes	was
T2420	2125-2129	NN	denotes	glue
T2419	2111-2124	NN	denotes	cyanoacrylate
T2418	2109-2110	-COMMA-	denotes	,
T2417	2102-2109	RB	denotes	quickly
T2416	2092-2101	VB	denotes	withdrawn
T2415	2088-2091	VB	denotes	was
T2414	2081-2087	NN	denotes	needle
T2413	2077-2080	DT	denotes	the
T2412	2075-2076	-COMMA-	denotes	,
T2411	2067-2075	NN	denotes	infusion
T2410	2063-2066	DT	denotes	the
T2409	2060-2062	IN	denotes	of
T2408	2049-2059	NN	denotes	completion
T2407	2045-2048	DT	denotes	the
T2406	2039-2044	IN	denotes	After
T2405	2033-2037	NN	denotes	pump
T2404	2019-2032	NN	denotes	microinfusion
T2403	2017-2018	DT	denotes	a
T2402	2014-2016	IN	denotes	of
T2401	2010-2013	NN	denotes	use
T2400	2006-2009	DT	denotes	the
T2399	2001-2005	IN	denotes	with
T2398	1991-2000	NN	denotes	20 μL/min
T2397	1988-1990	IN	denotes	of
T2396	1983-1987	NN	denotes	rate
T2395	1981-1982	DT	denotes	a
T2394	1978-1980	IN	denotes	at
T2393	1970-1977	VB	denotes	infused
T2392	1966-1969	VB	denotes	was
T2391	1960-1965	NN	denotes	blood
T2390	1954-1959	JJ	denotes	whole
T2389	1943-1953	JJ	denotes	autologous
T2388	1940-1942	IN	denotes	of
T2387	1934-1939	NN	denotes	75 μL
T2386	1932-1933	DT	denotes	a
T2385	1930-1931	-COMMA-	denotes	,
T2384	1926-1930	RB	denotes	Then
T2383	1923-1924	-RRB-	denotes	)
T2382	1916-1923	NN	denotes	midline
T2381	1912-1915	DT	denotes	the
T2380	1909-1911	TO	denotes	to
T2379	1901-1908	JJ	denotes	lateral
T2378	1894-1900	NN	denotes	3.0 mm
T2377	1890-1893	CC	denotes	and
T2376	1888-1889	-COMMA-	denotes	,
T2375	1881-1888	JJ	denotes	ventral
T2374	1874-1880	NN	denotes	6.0 mm
T2373	1872-1873	-COMMA-	denotes	,
T2372	1864-1872	JJ	denotes	anterior
T2371	1857-1863	CD	denotes	0.2 mm
T2370	1855-1856	-COLON-	denotes	:
T2369	1844-1855	NN	denotes	coordinates
T2368	1843-1844	-LRB-	denotes	(
T2367	1834-1842	NN	denotes	guidance
T2366	1821-1833	JJ	denotes	stereotactic
T2365	1815-1820	IN	denotes	under
T2364	1807-1814	NN	denotes	ganglia
T2363	1801-1806	JJ	denotes	basal
T2362	1796-1800	JJ	denotes	left
T2361	1792-1795	DT	denotes	the
T2360	1787-1791	IN	denotes	into
T2359	1778-1786	VB	denotes	inserted
T2358	1774-1777	VB	denotes	was
T2357	1767-1773	NN	denotes	needle
T2356	1758-1766	JJ	denotes	26-gauge
T2355	1752-1757	JJ	denotes	blunt
T2354	1750-1751	DT	denotes	A
T2353	1741-1748	NN	denotes	midline
T2352	1737-1740	DT	denotes	the
T2351	1734-1736	TO	denotes	to
T2350	1726-1733	JJ	denotes	lateral
T2349	1721-1725	NN	denotes	3 mm
T2348	1714-1720	NN	denotes	suture
T2347	1706-1713	JJ	denotes	coronal
T2346	1701-1705	JJ	denotes	left
T2345	1697-1700	DT	denotes	the
T2344	1694-1696	IN	denotes	on
T2343	1688-1693	NN	denotes	skull
T2342	1684-1687	DT	denotes	the
T2341	1681-1683	IN	denotes	in
T2340	1674-1680	VB	denotes	opened
T2339	1670-1673	VB	denotes	was
T2338	1665-1669	NN	denotes	hole
T2337	1660-1664	NN	denotes	burr
T2336	1655-1659	NN	denotes	1 mm
T2335	1653-1654	DT	denotes	a
T2334	1651-1652	-COMMA-	denotes	,
T2333	1642-1651	NN	denotes	technique
T2332	1634-1641	JJ	denotes	sterile
T2331	1632-1633	DT	denotes	a
T2330	1626-1631	VB	denotes	Using
T2329	1617-1624	NN	denotes	midline
T2328	1613-1616	DT	denotes	the
T2327	1607-1612	IN	denotes	along
T2326	1599-1606	VB	denotes	incised
T2325	1595-1598	VB	denotes	was
T2324	1589-1594	NN	denotes	scalp
T2323	1585-1588	DT	denotes	the
T2322	1581-1584	CC	denotes	and
T2321	1579-1580	-COMMA-	denotes	,
T2320	1574-1579	NN	denotes	frame
T2297	1424-1425	-LRB-	denotes	(
T2296	1419-1423	NN	denotes	body
T2295	1415-1418	DT	denotes	The
T2294	1412-1413	-RRB-	denotes	)
T2293	1404-1412	NN	denotes	60 mg/kg
T2292	1403-1404	-LRB-	denotes	(
T2291	1389-1402	NN	denotes	pentobarbital
T2290	1382-1388	NN	denotes	sodium
T2289	1365-1381	JJ	denotes	intra-peritoneal
T2288	1360-1364	IN	denotes	with
T2287	1347-1359	VB	denotes	anesthetized
T2286	1342-1346	VB	denotes	were
T2285	1337-1341	NN	denotes	Rats
T2284	1328-1335	NN	denotes	ganglia
T2283	1322-1327	JJ	denotes	basal
T2282	1317-1321	JJ	denotes	left
T2281	1314-1316	IN	denotes	in
T2280	1306-1313	VB	denotes	induced
T2279	1302-1305	VB	denotes	was
T2278	1298-1301	NN	denotes	ICH
T2277	1295-1297	IN	denotes	of
T2276	1289-1294	NN	denotes	model
T2275	1285-1288	NN	denotes	rat
T2274	1283-1284	-COMMA-	denotes	,
T2273	1278-1283	NN	denotes	study
T2272	1270-1277	JJ	denotes	present
T2271	1266-1269	DT	denotes	the
T2270	1263-1265	IN	denotes	in
T2269	1261-1262	-COMMA-	denotes	,
T2268	1251-1261	RB	denotes	clinically
T2267	1243-1250	NN	denotes	ganglia
T2266	1237-1242	JJ	denotes	basal
T2265	1233-1236	DT	denotes	the
T2264	1230-1232	IN	denotes	in
T2263	1224-1229	VB	denotes	occur
T2262	1212-1223	NN	denotes	hemorrhages
T2261	1198-1211	JJ	denotes	intracerebral
T2260	1193-1197	JJ	denotes	most
T2259	1187-1192	IN	denotes	Since
T2258	1176-1186	NNP	denotes	Hemorrhage
T2257	1162-1175	JJ	denotes	Intracerebral
T2256	1159-1161	IN	denotes	of
T2255	1149-1158	NN	denotes	Induction
T2254	1144-1147	CD	denotes	2.2
T2872	2600-2617	NN	denotes	Spectrophotometer
T2871	2592-2599	NN	denotes	ND-1000
T2870	2591-2592	-LRB-	denotes	(
T2869	2582-2590	NNP	denotes	Nanodrop
T2868	2580-2581	DT	denotes	a
T2867	2577-2579	IN	denotes	on
T2866	2566-2576	VB	denotes	determined
T2865	2562-2565	VB	denotes	was
T2864	2558-2561	NN	denotes	RNA
T2863	2552-2557	JJ	denotes	total
T2862	2549-2551	IN	denotes	of
T2861	2540-2548	NN	denotes	quantity
T2860	2536-2539	DT	denotes	The
T2859	2522-2534	NN	denotes	instructions
T2858	2519-2521	POS	denotes	's
T2857	2507-2519	NN	denotes	manufacturer
T2856	2503-2506	DT	denotes	the
T2855	2500-2502	TO	denotes	to
T2854	2490-2499	VB	denotes	according
T2853	2488-2489	-RRB-	denotes	)
T2852	2483-2488	NNP	denotes	China
T2851	2481-2482	-COMMA-	denotes	,
T2850	2479-2481	NNP	denotes	HZ
T2849	2477-2478	-COMMA-	denotes	,
T2848	2470-2477	NNP	denotes	BioFlux
T2847	2469-2470	-LRB-	denotes	(
T2846	2465-2468	NN	denotes	Kit
T2845	2455-2464	NN	denotes	isolation
T2844	2451-2454	NN	denotes	RNA
T2843	2445-2450	NNP	denotes	Total
T2842	2443-2444	NNP	denotes	P
T2841	2436-2442	NNP	denotes	Simply
T2840	2430-2435	VB	denotes	using
T2839	2422-2429	NN	denotes	ganglia
T2838	2416-2421	JJ	denotes	basal
T2837	2411-2415	JJ	denotes	left
T2836	2408-2410	IN	denotes	in
T2835	2400-2407	NN	denotes	regions
T2834	2384-2399	JJ	denotes	peri-hemorrhage
T2833	2379-2383	IN	denotes	from
T2832	2370-2378	VB	denotes	isolated
T2831	2365-2369	VB	denotes	were
T2830	2357-2364	NN	denotes	samples
T2829	2353-2356	NN	denotes	RNA
T2828	2347-2352	JJ	denotes	total
T2827	2345-2346	-COMMA-	denotes	,
T2826	2338-2345	RB	denotes	Briefly
T2825	2331-2337	NN	denotes	RT-PCR
T2824	2328-2330	IN	denotes	of
T2823	2321-2327	NN	denotes	Method
T2822	2317-2320	DT	denotes	the
T2821	2314-2316	IN	denotes	by
T2820	2309-2313	NN	denotes	mRNA
T2819	2304-2308	NN	denotes	TLR4
T2818	2301-2303	IN	denotes	of
T2817	2291-2300	NN	denotes	Detection
T2816	2286-2289	CD	denotes	2.3
T1988	1136-1141	NN	denotes	Blots
T1987	1128-1135	JJ	denotes	Western
T1986	1124-1127	CC	denotes	and
T1985	1117-1123	NN	denotes	RT-PCR
T1984	1113-1116	IN	denotes	for
T1983	1108-1112	NN	denotes	80°C
T1982	1102-1107	CC	denotes	minus
T1981	1099-1101	IN	denotes	at
T1980	1092-1098	VB	denotes	stored
T1979	1088-1091	CC	denotes	and
T1978	1080-1087	VB	denotes	removed
T1977	1075-1079	VB	denotes	were
T1976	1068-1074	NN	denotes	brains
T1975	1064-1067	DT	denotes	The
T1974	1054-1063	NNP	denotes	Ketamine.
T1973	1051-1053	IN	denotes	of
T1972	1041-1050	NN	denotes	injection
T1971	1036-1040	NN	denotes	dose
T1970	1031-1035	IN	denotes	over
T1969	1028-1030	IN	denotes	by
T1968	1017-1027	VB	denotes	sacrificed
T1967	1012-1016	VB	denotes	were
T1966	1006-1011	NN	denotes	point
T1965	1001-1005	NN	denotes	time
T1964	996-1000	DT	denotes	each
T1963	993-995	IN	denotes	at
T1962	987-992	NN	denotes	group
T1961	982-986	DT	denotes	each
T1960	979-981	IN	denotes	in
T1959	974-978	NN	denotes	rats
T1958	969-973	CD	denotes	Five
T1957	966-967	-RRB-	denotes	]
T1956	965-966	CD	denotes	8
T1955	964-965	-LRB-	denotes	[
T1954	953-963	RB	denotes	previously
T1953	943-952	VB	denotes	described
T1952	940-942	IN	denotes	as
T1951	938-939	-RRB-	denotes	)
T1950	934-938	NN	denotes	7 μm
T1949	933-934	-LRB-	denotes	(
T1948	924-932	NN	denotes	sections
T1947	919-923	IN	denotes	into
T1946	915-918	VB	denotes	cut
T1945	911-914	CC	denotes	and
T1944	909-910	-COMMA-	denotes	,
T1943	901-909	NN	denotes	paraffin
T1942	898-900	IN	denotes	in
T1941	889-897	VB	denotes	embedded
T1940	887-888	-COMMA-	denotes	,
T1939	878-887	VB	denotes	postfixed
T1938	876-877	-COMMA-	denotes	,
T1937	869-876	VB	denotes	removed
T1936	864-868	VB	denotes	were
T1935	857-863	NN	denotes	brains
T1934	853-856	DT	denotes	The
T1933	850-851	-RRB-	denotes	)
T1932	847-850	CD	denotes	7.4
T1931	845-846	SYM	denotes	=
T1930	842-844	NN	denotes	pH
T1929	841-842	-LRB-	denotes	(
T1928	828-840	NN	denotes	formaldehyde
T1927	823-827	NNP	denotes	Para
T1926	814-822	VB	denotes	buffered
T1925	812-813	NN	denotes	%
T1924	811-812	CD	denotes	4
T1923	808-810	IN	denotes	by
T1922	799-807	VB	denotes	followed
T1921	792-798	NN	denotes	saline
T1920	785-791	JJ	denotes	normal
T1919	782-784	IN	denotes	of
T1918	772-781	NN	denotes	perfusion
T1917	757-771	RB	denotes	transcardially
T1916	754-756	IN	denotes	by
T1915	743-753	VB	denotes	sacrificed
T1914	738-742	VB	denotes	were
T1913	732-737	NN	denotes	point
T1912	727-731	NN	denotes	time
T1911	722-726	DT	denotes	each
T1910	719-721	IN	denotes	at
T1909	713-718	NN	denotes	group
T1908	708-712	DT	denotes	each
T1907	705-707	IN	denotes	in
T1906	700-704	NN	denotes	rats
T1905	695-699	CD	denotes	Five
T1904	681-693	RB	denotes	respectively
T1903	679-680	-COMMA-	denotes	,
T1902	670-679	NN	denotes	injection
T1901	666-669	DT	denotes	the
T1900	660-665	IN	denotes	after
T1899	655-659	NN	denotes	days
T1898	653-654	CD	denotes	7
T1897	649-652	CC	denotes	and
T1896	644-648	NN	denotes	days
T1895	642-643	CD	denotes	3
T1894	640-641	-COMMA-	denotes	,
T1893	637-640	NN	denotes	day
T1892	635-636	CD	denotes	1
T1891	633-634	-COMMA-	denotes	,
T1890	626-633	NN	denotes	6 hours
T1889	623-625	IN	denotes	on
T1888	612-622	VB	denotes	sacrificed
T1887	607-611	VB	denotes	were
T1886	603-606	CC	denotes	and
T1885	597-602	NN	denotes	blood
T1884	591-596	JJ	denotes	whole
T1883	580-590	JJ	denotes	autologous
T1882	577-579	CC	denotes	or
T1881	570-576	NN	denotes	saline
T1880	563-569	JJ	denotes	normal
T1879	560-562	IN	denotes	of
T1878	550-559	NN	denotes	injection
T1877	535-549	JJ	denotes	intra-cerebral
T1876	532-534	TO	denotes	to
T1875	522-531	VB	denotes	subjected
T1874	517-521	VB	denotes	were
T1873	512-516	NN	denotes	rats
T1872	508-511	DT	denotes	The
T1871	496-507	NNP	denotes	University.
T1870	488-495	NNP	denotes	Medical
T1869	482-487	NNP	denotes	China
T1868	480-481	-COMMA-	denotes	,
T1867	471-480	NNP	denotes	Committee
T1866	467-470	NNP	denotes	Use
T1865	463-466	CC	denotes	and
T1864	458-462	NNP	denotes	Care
T1863	451-457	NNP	denotes	Animal
T1862	447-450	DT	denotes	the
T1861	444-446	IN	denotes	by
T1860	435-443	VB	denotes	approved
T1859	431-434	VB	denotes	was
T1858	423-430	NN	denotes	animals
T1857	419-422	IN	denotes	for
T1856	414-418	VB	denotes	used
T1855	405-413	NN	denotes	protocol
T1854	392-404	JJ	denotes	experimental
T1853	388-391	DT	denotes	The
T1852	385-386	-RRB-	denotes	)
T1851	383-385	CD	denotes	40
T1850	381-382	SYM	denotes	=
T1849	379-380	NN	denotes	n
T1848	377-378	-COMMA-	denotes	,
T1847	374-377	NN	denotes	ICH
T2319	1561-1573	JJ	denotes	stereotactic
T2318	1559-1560	DT	denotes	a
T2317	1556-1558	IN	denotes	in
T2316	1545-1555	VB	denotes	positioned
T2315	1540-1544	VB	denotes	were
T2314	1535-1539	NN	denotes	rats
T2313	1531-1534	DT	denotes	The
T2312	1523-1529	NN	denotes	system
T2311	1515-1522	NN	denotes	heating
T2310	1495-1514	JJ	denotes	feedback-controlled
T2309	1493-1494	DT	denotes	a
T2308	1487-1492	VB	denotes	using
T2307	1479-1486	NN	denotes	surgery
T2306	1475-1478	DT	denotes	the
T2305	1468-1474	IN	denotes	during
T2304	1463-1467	NN	denotes	37°C
T2303	1460-1462	IN	denotes	at
T2302	1449-1459	VB	denotes	maintained
T2301	1445-1448	VB	denotes	was
T2300	1433-1444	NN	denotes	temperature
T2299	1431-1432	-RRB-	denotes	)
T2298	1425-1431	JJ	denotes	rectal
T1846	373-374	-LRB-	denotes	(
T1845	367-372	NN	denotes	group
T1844	356-366	NN	denotes	hemorrhage
T1843	341-355	JJ	denotes	intra-cerebral
T1842	337-340	CC	denotes	and
T1841	335-336	-RRB-	denotes	)
T1840	328-335	NN	denotes	control
T1839	325-327	IN	denotes	as
T1838	318-324	NN	denotes	saline
T1837	311-317	JJ	denotes	normal
T1836	308-310	IN	denotes	of
T1835	298-307	NN	denotes	injection
T1834	283-297	JJ	denotes	intra-cerebral
T1833	281-282	-COMMA-	denotes	,
T1832	279-281	CD	denotes	40
T1831	277-278	SYM	denotes	=
T1830	275-276	NN	denotes	n
T1829	273-274	-COMMA-	denotes	,
T1828	272-273	NN	denotes	S
T1827	271-272	-LRB-	denotes	(
T1826	263-270	NN	denotes	control
T1825	256-262	NN	denotes	saline
T1824	249-255	JJ	denotes	normal
T1823	247-248	-COMMA-	denotes	,
T1822	246-247	-RRB-	denotes	)
T1821	237-246	NN	denotes	treatment
T1820	233-236	DT	denotes	any
T1819	225-232	IN	denotes	without
T1818	223-224	-COMMA-	denotes	,
T1817	221-223	CD	denotes	10
T1816	219-220	SYM	denotes	=
T1815	217-218	NN	denotes	n
T1814	215-216	-COMMA-	denotes	,
T1813	214-215	NN	denotes	N
T1812	213-214	-LRB-	denotes	(
T1811	207-212	NN	denotes	group
T1810	200-206	JJ	denotes	normal
T1809	198-199	-COLON-	denotes	:
T1808	192-198	NN	denotes	groups
T1807	186-191	CD	denotes	three
T1806	183-185	TO	denotes	to
T1805	174-182	RB	denotes	randomly
T1804	165-173	VB	denotes	assigned
T1803	160-164	VB	denotes	were
T1802	155-159	NN	denotes	rats
T1801	151-154	DT	denotes	The
T1800	139-150	NNP	denotes	University.
T1799	131-138	NNP	denotes	Medical
T1798	125-130	NNP	denotes	China
T1797	123-124	-COMMA-	denotes	,
T1796	117-123	NNP	denotes	Center
T1795	110-116	NNP	denotes	Animal
T1794	97-109	NNP	denotes	Experimental
T1793	94-96	IN	denotes	by
T1792	85-93	VB	denotes	provided
T1791	80-84	VB	denotes	were
T1790	78-79	-RRB-	denotes	)
T1789	73-78	CD	denotes	300 g
T1788	70-72	TO	denotes	to
T1787	64-69	CD	denotes	250 g
T1786	62-63	-COLON-	denotes	:
T1785	56-62	NN	denotes	weight
T1784	51-55	NN	denotes	body
T1783	49-50	-COMMA-	denotes	,
T1782	45-49	NN	denotes	male
T1781	44-45	-LRB-	denotes	(
T1780	39-43	NN	denotes	rats
T1779	32-38	NNP	denotes	Wister
T1778	25-31	NNP	denotes	Ninety
T1777	17-24	NN	denotes	Animals
T1776	12-15	CD	denotes	2.1
R1324	T1779	T1778	arg1Of	Wister,Ninety
R1325	T1780	T1779	arg1Of	rats,Wister
R1326	T1780	T1781	arg1Of	rats,(
R1327	T1782	T1781	arg2Of	male,(
R1328	T1782	T1783	arg1Of	male,","
R1329	T1785	T1783	arg2Of	weight,","
R1330	T1785	T1784	arg1Of	weight,body
R1331	T1786	T1781	arg3Of	:,(
R1332	T1787	T1788	arg1Of	250 g,to
R1333	T1789	T1788	arg2Of	300 g,to
R1334	T1792	T1791	arg2Of	provided,were
R1335	T1796	T1794	arg1Of	Center,Experimental
R1336	T1796	T1795	arg1Of	Center,Animal
R1337	T1796	T1797	arg1Of	Center,","
R1338	T1799	T1797	arg2Of	Medical,","
R1339	T1799	T1798	arg1Of	Medical,China
R1340	T1800	T1792	arg1Of	University.,provided
R1341	T1800	T1793	arg2Of	University.,by
R1342	T1800	T1796	arg1Of	University.,Center
R1343	T1800	T1799	arg1Of	University.,Medical
R1344	T1802	T1801	arg1Of	rats,The
R1345	T1802	T1803	arg1Of	rats,were
R1346	T1802	T1804	arg2Of	rats,assigned
R1347	T1804	T1803	arg2Of	assigned,were
R1348	T1804	T1805	arg1Of	assigned,randomly
R1349	T1804	T1806	arg1Of	assigned,to
R1350	T1808	T1806	arg2Of	groups,to
R1351	T1808	T1807	arg1Of	groups,three
R1352	T1811	T1810	arg1Of	group,normal
R1353	T1811	T1812	arg1Of	group,(
R1354	T1811	T1823	arg1Of	group,","
R1355	T1813	T1812	arg2Of	N,(
R1356	T1813	T1814	arg1Of	N,","
R1357	T1813	T1816	arg1Of	N,=
R1358	T1813	T1819	arg1Of	N,without
R1359	T1815	T1814	arg2Of	n,","
R1360	T1816	T1817	arg1Of	=,10
R1361	T1816	T1818	arg1Of	=,","
R1362	T1821	T1819	arg2Of	treatment,without
R1363	T1821	T1820	arg1Of	treatment,any
R1364	T1822	T1812	arg3Of	),(
R1365	T1823	T1809	arg2Of	",",:
R1366	T1826	T1823	arg2Of	control,","
R1367	T1826	T1824	arg1Of	control,normal
R1368	T1826	T1825	arg1Of	control,saline
R1369	T1828	T1829	arg1Of	S,","
R1370	T1830	T1829	arg2Of	n,","
R1371	T1832	T1831	arg1Of	40,=
R1372	T1832	T1833	arg1Of	40,","
R1373	T1834	T1833	arg2Of	intra-cerebral,","
R1374	T1835	T1832	arg1Of	injection,40
R1375	T1835	T1834	arg1Of	injection,intra-cerebral
R1376	T1835	T1836	arg1Of	injection,of
R1377	T1838	T1836	arg2Of	saline,of
R1378	T1838	T1837	arg1Of	saline,normal
R1379	T1840	T1839	arg2Of	control,as
R1380	T1845	T1842	arg2Of	group,and
R1381	T1845	T1843	arg1Of	group,intra-cerebral
R1382	T1845	T1844	arg1Of	group,hemorrhage
R1383	T1845	T1846	arg1Of	group,(
R1384	T1847	T1846	arg2Of	ICH,(
R1385	T1847	T1848	arg1Of	ICH,","
R1386	T1849	T1848	arg2Of	n,","
R1387	T1849	T1850	arg1Of	n,=
R1388	T1849	T1851	arg1Of	n,40
R1389	T1852	T1846	arg3Of	),(
R1390	T1855	T1853	arg1Of	protocol,The
R1391	T1855	T1854	arg1Of	protocol,experimental
R1392	T1855	T1856	arg2Of	protocol,used
R1393	T1855	T1859	arg1Of	protocol,was
R1394	T1855	T1860	arg2Of	protocol,approved
R1395	T1856	T1857	arg1Of	used,for
R1396	T1858	T1857	arg2Of	animals,for
R1397	T1860	T1859	arg2Of	approved,was
R1398	T1864	T1863	arg1Of	Care,Animal
R1399	T1864	T1865	arg1Of	Care,and
R1400	T1865	T1860	arg1Of	and,approved
R1401	T1865	T1861	arg2Of	and,by
R1402	T1865	T1862	arg1Of	and,the
R1403	T1867	T1865	arg2Of	Committee,and
R1404	T1867	T1866	arg1Of	Committee,Use
R1405	T1871	T1869	arg1Of	University.,China
R1406	T1871	T1870	arg1Of	University.,Medical
R1407	T1873	T1871	arg1Of	rats,University.
R1408	T1873	T1872	arg1Of	rats,The
R1409	T1873	T1874	arg1Of	rats,were
R1410	T1873	T1875	arg2Of	rats,subjected
R1411	T1873	T1887	arg1Of	rats,were
R1412	T1873	T1888	arg2Of	rats,sacrificed
R1413	T1875	T1874	arg2Of	subjected,were
R1414	T1875	T1876	arg1Of	subjected,to
R1415	T1875	T1886	arg1Of	subjected,and
R1416	T1878	T1876	arg2Of	injection,to
R1417	T1878	T1877	arg1Of	injection,intra-cerebral
R1418	T1878	T1879	arg1Of	injection,of
R1419	T1881	T1880	arg1Of	saline,normal
R1420	T1881	T1882	arg1Of	saline,or
R1421	T1882	T1879	arg2Of	or,of
R1422	T1885	T1882	arg2Of	blood,or
R1423	T1885	T1883	arg1Of	blood,autologous
R1424	T1885	T1884	arg1Of	blood,whole
R1425	T1886	T1859	modOf	and,was
R1426	T1886	T1868	arg1Of	and,","
R1427	T1888	T1886	arg2Of	sacrificed,and
R1428	T1888	T1887	arg2Of	sacrificed,were
R1429	T1888	T1889	arg1Of	sacrificed,on
R1430	T1888	T1900	arg1Of	sacrificed,after
R1431	T1888	T1903	arg1Of	sacrificed,","
R1432	T1888	T1904	arg1Of	sacrificed,respectively
R1433	T1890	T1891	arg1Of	6 hours,","
R1434	T1891	T1894	arg1Of	",",","
R1435	T1893	T1891	arg2Of	day,","
R1436	T1893	T1892	arg1Of	day,1
R1437	T1894	T1897	arg1Of	",",and
R1438	T1896	T1894	arg2Of	days,","
R1439	T1896	T1895	arg1Of	days,3
R1440	T1897	T1889	arg2Of	and,on
R1441	T1899	T1897	arg2Of	days,and
R1442	T1899	T1898	arg1Of	days,7
R1443	T1902	T1900	arg2Of	injection,after
R1444	T1902	T1901	arg1Of	injection,the
R1445	T1906	T1905	arg1Of	rats,Five
R1446	T1906	T1907	arg1Of	rats,in
R1447	T1906	T1926	arg1Of	rats,buffered
R1448	T1909	T1907	arg2Of	group,in
R1449	T1909	T1908	arg1Of	group,each
R1450	T1909	T1910	arg1Of	group,at
R1451	T1912	T1910	arg2Of	time,at
R1452	T1912	T1911	arg1Of	time,each
R1453	T1913	T1914	arg1Of	point,were
R1454	T1913	T1915	arg2Of	point,sacrificed
R1455	T1915	T1912	arg2Of	sacrificed,time
R1456	T1915	T1914	arg2Of	sacrificed,were
R1457	T1918	T1915	arg1Of	perfusion,sacrificed
R1458	T1918	T1916	arg2Of	perfusion,by
R1459	T1918	T1917	arg1Of	perfusion,transcardially
R1460	T1918	T1919	arg1Of	perfusion,of
R1461	T1921	T1919	arg2Of	saline,of
R1462	T1921	T1920	arg1Of	saline,normal
R1463	T1921	T1922	arg2Of	saline,followed
R1464	T1922	T1923	arg1Of	followed,by
R1465	T1925	T1923	arg2Of	%,by
R1466	T1925	T1924	arg1Of	%,4
R1467	T1928	T1926	arg2Of	formaldehyde,buffered
R1468	T1928	T1927	arg1Of	formaldehyde,Para
R1469	T1928	T1929	arg1Of	formaldehyde,(
R1470	T1930	T1929	arg2Of	pH,(
R1471	T1930	T1931	arg1Of	pH,=
R1472	T1930	T1932	arg1Of	pH,7.4
R1473	T1933	T1929	arg3Of	),(
R1474	T1935	T1934	arg1Of	brains,The
R1475	T1935	T1936	arg1Of	brains,were
R1476	T1935	T1937	arg2Of	brains,removed
R1477	T1935	T1939	arg2Of	brains,postfixed
R1478	T1935	T1941	arg2Of	brains,embedded
R1479	T1935	T1946	arg1Of	brains,cut
R1480	T1937	T1938	arg1Of	removed,","
R1481	T1938	T1940	arg1Of	",",","
R1482	T1939	T1938	arg2Of	postfixed,","
R1483	T1940	T1936	arg2Of	",",were
R1484	T1940	T1945	arg1Of	",",and
R1485	T1941	T1940	arg2Of	embedded,","
R1486	T1941	T1942	arg1Of	embedded,in
R1487	T1943	T1942	arg2Of	paraffin,in
R1488	T1945	T1944	arg1Of	and,","
R1489	T1946	T1945	arg2Of	cut,and
R1490	T1946	T1947	arg1Of	cut,into
R1491	T1948	T1947	arg2Of	sections,into
R1492	T1948	T1949	arg1Of	sections,(
R1493	T1948	T1952	arg1Of	sections,as
R1494	T1950	T1949	arg2Of	7 μm,(
R1495	T1951	T1949	arg3Of	),(
R1496	T1953	T1952	arg2Of	described,as
R1497	T1953	T1954	arg1Of	described,previously
R1498	T1953	T1955	arg1Of	described,[
R1499	T1956	T1955	arg2Of	8,[
R1500	T1957	T1955	arg3Of	],[
R1501	T1959	T1958	arg1Of	rats,Five
R1502	T1959	T1960	arg1Of	rats,in
R1503	T1959	T1977	arg1Of	rats,were
R1504	T1959	T1978	arg2Of	rats,removed
R1505	T1959	T1980	arg2Of	rats,stored
R1506	T1962	T1960	arg2Of	group,in
R1507	T1962	T1961	arg1Of	group,each
R1508	T1962	T1963	arg1Of	group,at
R1509	T1965	T1963	arg2Of	time,at
R1510	T1965	T1964	arg1Of	time,each
R1511	T1966	T1967	arg1Of	point,were
R1512	T1966	T1968	arg2Of	point,sacrificed
R1513	T1968	T1965	arg2Of	sacrificed,time
R1514	T1968	T1967	arg2Of	sacrificed,were
R1515	T1972	T1968	arg1Of	injection,sacrificed
R1516	T1972	T1969	arg2Of	injection,by
R1517	T1972	T1970	arg1Of	injection,over
R1518	T1972	T1971	arg1Of	injection,dose
R1519	T1972	T1973	arg1Of	injection,of
R1520	T1976	T1973	arg2Of	brains,of
R1521	T1976	T1974	arg1Of	brains,Ketamine.
R1522	T1976	T1975	arg1Of	brains,The
R1523	T1978	T1979	arg1Of	removed,and
R1524	T1979	T1977	arg2Of	and,were
R1525	T1980	T1979	arg2Of	stored,and
R1526	T1980	T1981	arg1Of	stored,at
R1527	T1983	T1981	arg2Of	80°C,at
R1528	T1983	T1982	arg1Of	80°C,minus
R1529	T1983	T1984	arg1Of	80°C,for
R1530	T1985	T1986	arg1Of	RT-PCR,and
R1531	T1986	T1984	arg2Of	and,for
R1532	T1988	T1986	arg2Of	Blots,and
R1533	T1988	T1987	arg1Of	Blots,Western
R1763	T2255	T2256	arg1Of	Induction,of
R1764	T2258	T2256	arg2Of	Hemorrhage,of
R1765	T2258	T2257	arg1Of	Hemorrhage,Intracerebral
R1766	T2262	T2260	arg1Of	hemorrhages,most
R1767	T2262	T2261	arg1Of	hemorrhages,intracerebral
R1768	T2262	T2263	arg1Of	hemorrhages,occur
R1769	T2263	T2259	arg2Of	occur,Since
R1770	T2263	T2264	arg1Of	occur,in
R1771	T2267	T2264	arg2Of	ganglia,in
R1772	T2267	T2265	arg1Of	ganglia,the
R1773	T2267	T2266	arg1Of	ganglia,basal
R1774	T2267	T2268	arg1Of	ganglia,clinically
R1775	T2273	T2270	arg2Of	study,in
R1776	T2273	T2271	arg1Of	study,the
R1777	T2273	T2272	arg1Of	study,present
R1778	T2276	T2275	arg1Of	model,rat
R1779	T2276	T2277	arg1Of	model,of
R1780	T2276	T2279	arg1Of	model,was
R1781	T2276	T2280	arg2Of	model,induced
R1782	T2278	T2277	arg2Of	ICH,of
R1783	T2280	T2259	arg1Of	induced,Since
R1784	T2280	T2269	arg1Of	induced,","
R1785	T2280	T2270	arg1Of	induced,in
R1786	T2280	T2274	arg1Of	induced,","
R1787	T2280	T2279	arg2Of	induced,was
R1788	T2280	T2281	arg1Of	induced,in
R1789	T2284	T2281	arg2Of	ganglia,in
R1790	T2284	T2282	arg1Of	ganglia,left
R1791	T2284	T2283	arg1Of	ganglia,basal
R1792	T2285	T2286	arg1Of	Rats,were
R1793	T2285	T2287	arg2Of	Rats,anesthetized
R1794	T2287	T2286	arg2Of	anesthetized,were
R1795	T2287	T2288	arg1Of	anesthetized,with
R1796	T2291	T2288	arg2Of	pentobarbital,with
R1797	T2291	T2289	arg1Of	pentobarbital,intra-peritoneal
R1798	T2291	T2290	arg1Of	pentobarbital,sodium
R1799	T2291	T2292	arg1Of	pentobarbital,(
R1800	T2293	T2292	arg2Of	60 mg/kg,(
R1801	T2294	T2292	arg3Of	),(
R1802	T2298	T2297	arg2Of	rectal,(
R1803	T2299	T2297	arg3Of	),(
R1804	T2300	T2295	arg1Of	temperature,The
R1805	T2300	T2296	arg1Of	temperature,body
R1806	T2300	T2297	arg1Of	temperature,(
R1807	T2300	T2301	arg1Of	temperature,was
R1808	T2300	T2302	arg2Of	temperature,maintained
R1809	T2302	T2301	arg2Of	maintained,was
R1810	T2302	T2303	arg1Of	maintained,at
R1811	T2302	T2305	arg1Of	maintained,during
R1812	T2304	T2303	arg2Of	37°C,at
R1813	T2307	T2305	arg2Of	surgery,during
R1814	T2307	T2306	arg1Of	surgery,the
R1815	T2307	T2308	arg1Of	surgery,using
R1816	T2312	T2308	arg2Of	system,using
R1817	T2312	T2309	arg1Of	system,a
R1818	T2312	T2310	arg1Of	system,feedback-controlled
R1819	T2312	T2311	arg1Of	system,heating
R1820	T2314	T2313	arg1Of	rats,The
R1821	T2314	T2315	arg1Of	rats,were
R1822	T2314	T2316	arg2Of	rats,positioned
R1823	T2316	T2315	arg2Of	positioned,were
R1824	T2316	T2317	arg1Of	positioned,in
R1825	T2316	T2322	arg1Of	positioned,and
R1826	T2320	T2317	arg2Of	frame,in
R1827	T2320	T2318	arg1Of	frame,a
R1828	T2320	T2319	arg1Of	frame,stereotactic
R1829	T2322	T2321	arg1Of	and,","
R1830	T2324	T2323	arg1Of	scalp,the
R1831	T2324	T2325	arg1Of	scalp,was
R1832	T2324	T2326	arg2Of	scalp,incised
R1833	T2326	T2322	arg2Of	incised,and
R1834	T2326	T2325	arg2Of	incised,was
R1835	T2326	T2327	arg1Of	incised,along
R1836	T2329	T2327	arg2Of	midline,along
R1837	T2329	T2328	arg1Of	midline,the
R1838	T2333	T2330	arg2Of	technique,Using
R1839	T2333	T2331	arg1Of	technique,a
R1840	T2333	T2332	arg1Of	technique,sterile
R1841	T2338	T2330	arg1Of	hole,Using
R1842	T2338	T2335	arg1Of	hole,a
R1843	T2338	T2336	arg1Of	hole,1 mm
R1844	T2338	T2337	arg1Of	hole,burr
R1845	T2338	T2339	arg1Of	hole,was
R1846	T2338	T2340	arg2Of	hole,opened
R1847	T2340	T2330	modOf	opened,Using
R1848	T2340	T2334	arg1Of	opened,","
R1849	T2340	T2339	arg2Of	opened,was
R1850	T2340	T2341	arg1Of	opened,in
R1851	T2343	T2341	arg2Of	skull,in
R1852	T2343	T2342	arg1Of	skull,the
R1853	T2343	T2344	arg1Of	skull,on
R1854	T2349	T2344	arg2Of	3 mm,on
R1855	T2349	T2345	arg1Of	3 mm,the
R1856	T2349	T2346	arg1Of	3 mm,left
R1857	T2349	T2347	arg1Of	3 mm,coronal
R1858	T2349	T2348	arg1Of	3 mm,suture
R1859	T2349	T2350	arg1Of	3 mm,lateral
R1860	T2350	T2351	arg1Of	lateral,to
R1861	T2353	T2351	arg2Of	midline,to
R1862	T2353	T2352	arg1Of	midline,the
R1863	T2357	T2354	arg1Of	needle,A
R1864	T2357	T2355	arg1Of	needle,blunt
R1865	T2357	T2356	arg1Of	needle,26-gauge
R1866	T2357	T2358	arg1Of	needle,was
R1867	T2357	T2359	arg2Of	needle,inserted
R1868	T2359	T2358	arg2Of	inserted,was
R1869	T2359	T2360	arg1Of	inserted,into
R1870	T2359	T2365	arg1Of	inserted,under
R1871	T2364	T2360	arg2Of	ganglia,into
R1872	T2364	T2361	arg1Of	ganglia,the
R1873	T2364	T2362	arg1Of	ganglia,left
R1874	T2364	T2363	arg1Of	ganglia,basal
R1875	T2367	T2365	arg2Of	guidance,under
R1876	T2367	T2366	arg1Of	guidance,stereotactic
R1877	T2367	T2368	arg1Of	guidance,(
R1878	T2369	T2368	arg2Of	coordinates,(
R1879	T2370	T2368	arg3Of	:,(
R1880	T2371	T2372	arg1Of	0.2 mm,anterior
R1881	T2371	T2375	arg1Of	0.2 mm,ventral
R1882	T2371	T2377	arg1Of	0.2 mm,and
R1883	T2372	T2373	arg1Of	anterior,","
R1884	T2375	T2373	arg2Of	ventral,","
R1885	T2375	T2374	arg1Of	ventral,6.0 mm
R1886	T2377	T2376	arg1Of	and,","
R1887	T2378	T2377	arg2Of	3.0 mm,and
R1888	T2378	T2379	arg1Of	3.0 mm,lateral
R1889	T2379	T2380	arg1Of	lateral,to
R1890	T2382	T2380	arg2Of	midline,to
R1891	T2382	T2381	arg1Of	midline,the
R1892	T2382	T2383	arg1Of	midline,)
R1893	T2387	T2386	arg1Of	75 μL,a
R1894	T2387	T2388	arg1Of	75 μL,of
R1895	T2387	T2392	arg1Of	75 μL,was
R1896	T2387	T2393	arg2Of	75 μL,infused
R1897	T2391	T2388	arg2Of	blood,of
R1898	T2391	T2389	arg1Of	blood,autologous
R1899	T2391	T2390	arg1Of	blood,whole
R1900	T2393	T2384	arg1Of	infused,Then
R1901	T2393	T2385	arg1Of	infused,","
R1902	T2393	T2392	arg2Of	infused,was
R1903	T2393	T2394	arg1Of	infused,at
R1904	T2396	T2394	arg2Of	rate,at
R1905	T2396	T2395	arg1Of	rate,a
R1906	T2396	T2397	arg1Of	rate,of
R1907	T2396	T2399	arg1Of	rate,with
R1908	T2398	T2397	arg2Of	20 μL/min,of
R1909	T2401	T2399	arg2Of	use,with
R1910	T2401	T2400	arg1Of	use,the
R1911	T2401	T2402	arg1Of	use,of
R1912	T2405	T2402	arg2Of	pump,of
R1913	T2405	T2403	arg1Of	pump,a
R1914	T2405	T2404	arg1Of	pump,microinfusion
R1915	T2408	T2406	arg2Of	completion,After
R1916	T2408	T2407	arg1Of	completion,the
R1917	T2408	T2409	arg1Of	completion,of
R1918	T2411	T2409	arg2Of	infusion,of
R1919	T2411	T2410	arg1Of	infusion,the
R1920	T2414	T2413	arg1Of	needle,the
R1921	T2414	T2415	arg1Of	needle,was
R1922	T2414	T2416	arg2Of	needle,withdrawn
R1923	T2416	T2415	arg2Of	withdrawn,was
R1924	T2416	T2417	arg1Of	withdrawn,quickly
R1925	T2420	T2419	arg1Of	glue,cyanoacrylate
R1926	T2420	T2421	arg1Of	glue,was
R1927	T2420	T2422	arg2Of	glue,placed
R1928	T2422	T2421	arg2Of	placed,was
R1929	T2422	T2423	arg1Of	placed,around
R1930	T2422	T2428	arg1Of	placed,and
R1931	T2426	T2423	arg2Of	hole,around
R1932	T2426	T2424	arg1Of	hole,the
R1933	T2426	T2425	arg1Of	hole,burr
R1934	T2428	T2406	arg1Of	and,After
R1935	T2428	T2412	arg1Of	and,","
R1936	T2428	T2415	modOf	and,was
R1937	T2428	T2418	arg1Of	and,","
R1938	T2428	T2427	arg1Of	and,","
R1939	T2431	T2429	arg1Of	incision,the
R1940	T2431	T2430	arg1Of	incision,skin
R1941	T2431	T2432	arg1Of	incision,was
R1942	T2431	T2433	arg2Of	incision,closed
R1943	T2433	T2428	arg2Of	closed,and
R1944	T2433	T2432	arg2Of	closed,was
R1945	T2433	T2434	arg1Of	closed,with
R1946	T2435	T2434	arg2Of	sutures,with
R1947	T2441	T2436	arg2Of	rats,For
R1948	T2441	T2437	arg1Of	rats,the
R1949	T2441	T2438	arg1Of	rats,normal
R1950	T2441	T2439	arg1Of	rats,saline
R1951	T2441	T2440	arg1Of	rats,control
R1952	T2446	T2443	arg1Of	saline,only
R1953	T2446	T2444	arg1Of	saline,75 μL
R1954	T2446	T2445	arg1Of	saline,normal
R1955	T2446	T2447	arg1Of	saline,was
R1956	T2446	T2448	arg2Of	saline,injected
R1957	T2448	T2436	arg1Of	injected,For
R1958	T2448	T2442	arg1Of	injected,","
R1959	T2448	T2447	arg2Of	injected,was
R2188	T2817	T2818	arg1Of	Detection,of
R2189	T2817	T2821	arg1Of	Detection,by
R2190	T2820	T2818	arg2Of	mRNA,of
R2191	T2820	T2819	arg1Of	mRNA,TLR4
R2192	T2823	T2821	arg2Of	Method,by
R2193	T2823	T2822	arg1Of	Method,the
R2194	T2823	T2824	arg1Of	Method,of
R2195	T2825	T2824	arg2Of	RT-PCR,of
R2196	T2830	T2828	arg1Of	samples,total
R2197	T2830	T2829	arg1Of	samples,RNA
R2198	T2830	T2831	arg1Of	samples,were
R2199	T2830	T2832	arg2Of	samples,isolated
R2200	T2832	T2826	arg1Of	isolated,Briefly
R2201	T2832	T2827	arg1Of	isolated,","
R2202	T2832	T2831	arg2Of	isolated,were
R2203	T2832	T2833	arg1Of	isolated,from
R2204	T2835	T2833	arg2Of	regions,from
R2205	T2835	T2834	arg1Of	regions,peri-hemorrhage
R2206	T2835	T2836	arg1Of	regions,in
R2207	T2839	T2836	arg2Of	ganglia,in
R2208	T2839	T2837	arg1Of	ganglia,left
R2209	T2839	T2838	arg1Of	ganglia,basal
R2210	T2839	T2840	arg1Of	ganglia,using
R2211	T2840	T2847	arg1Of	using,(
R2212	T2840	T2854	arg1Of	using,according
R2213	T2843	T2841	arg1Of	Total,Simply
R2214	T2843	T2842	arg1Of	Total,P
R2215	T2846	T2840	arg2Of	Kit,using
R2216	T2846	T2843	arg1Of	Kit,Total
R2217	T2846	T2844	arg1Of	Kit,RNA
R2218	T2846	T2845	arg1Of	Kit,isolation
R2219	T2848	T2849	arg1Of	BioFlux,","
R2220	T2849	T2847	arg2Of	",",(
R2221	T2849	T2851	arg1Of	",",","
R2222	T2850	T2849	arg2Of	HZ,","
R2223	T2852	T2851	arg2Of	China,","
R2224	T2853	T2847	arg3Of	),(
R2225	T2855	T2854	arg2Of	to,according
R2226	T2857	T2856	arg1Of	manufacturer,the
R2227	T2857	T2858	arg2Of	manufacturer,'s
R2228	T2859	T2855	arg2Of	instructions,to
R2229	T2859	T2858	arg1Of	instructions,'s
R2230	T2861	T2860	arg1Of	quantity,The
R2231	T2861	T2862	arg1Of	quantity,of
R2232	T2861	T2865	arg1Of	quantity,was
R2233	T2861	T2866	arg2Of	quantity,determined
R2234	T2861	T2875	arg1Of	quantity,measuring
R2235	T2864	T2862	arg2Of	RNA,of
R2236	T2864	T2863	arg1Of	RNA,total
R2237	T2866	T2865	arg2Of	determined,was
R2238	T2866	T2867	arg1Of	determined,on
R2239	T2866	T2874	arg1Of	determined,by
R2240	T2869	T2867	arg2Of	Nanodrop,on
R2241	T2869	T2868	arg1Of	Nanodrop,a
R2242	T2869	T2870	arg1Of	Nanodrop,(
R2243	T2872	T2870	arg2Of	Spectrophotometer,(
R2244	T2872	T2871	arg1Of	Spectrophotometer,ND-1000
R2245	T2873	T2870	arg3Of	),(
R2246	T2875	T2874	arg2Of	measuring,by
R2247	T2875	T2878	arg1Of	measuring,at
R2248	T2877	T2875	arg2Of	density,measuring
R2249	T2877	T2876	arg1Of	density,optical
R2250	T2879	T2880	arg1Of	A260,and
R2251	T2881	T2880	arg2Of	A280 nm,and
R2252	T2882	T2878	arg2Of	wavelength,at
R2253	T2882	T2879	arg1Of	wavelength,A260
R2254	T2882	T2881	arg1Of	wavelength,A280 nm
R2255	T2886	T2885	arg1Of	concentrations,the
R2256	T2886	T2887	arg1Of	concentrations,of
R2257	T2886	T2889	arg1Of	concentrations,were
R2258	T2886	T2890	arg2Of	concentrations,adjusted
R2259	T2888	T2887	arg2Of	mRNA,of
R2260	T2890	T2883	arg1Of	adjusted,Then
R2261	T2890	T2884	arg1Of	adjusted,","
R2262	T2890	T2889	arg2Of	adjusted,were
R2263	T2890	T2891	arg1Of	adjusted,to
R2264	T2892	T2891	arg2Of	1.0 g/mL,to
R2265	T2893	T2894	arg1Of	RNA,was
R2266	T2893	T2895	arg2Of	RNA,reverse-transcribed
R2267	T2893	T2902	arg1Of	RNA,using
R2268	T2895	T2894	arg2Of	reverse-transcribed,was
R2269	T2895	T2896	arg1Of	reverse-transcribed,into
R2270	T2895	T2898	arg1Of	reverse-transcribed,in
R2271	T2895	T2902	modOf	reverse-transcribed,using
R2272	T2897	T2896	arg2Of	cDNA,into
R2273	T2901	T2898	arg2Of	system,in
R2274	T2901	T2899	arg1Of	system,20 μL
R2275	T2901	T2900	arg1Of	system,reaction
R2276	T2905	T2903	arg1Of	Synthesis,Superscript
R2277	T2905	T2904	arg1Of	Synthesis,First-Strand
R2278	T2906	T2902	arg2Of	Kit,using
R2279	T2906	T2905	arg1Of	Kit,Synthesis
R2280	T2906	T2907	arg1Of	Kit,for
R2281	T2908	T2907	arg2Of	RT-PCR,for
R2282	T2908	T2909	arg1Of	RT-PCR,(
R2283	T2908	T2913	arg1Of	RT-PCR,under
R2284	T2911	T2909	arg2Of	Inc,(
R2285	T2911	T2910	arg1Of	Inc,promega
R2286	T2912	T2909	arg3Of	),(
R2287	T2914	T2913	arg2Of	conditions,under
R2288	T2914	T2915	arg2Of	conditions,described
R2289	T2918	T2915	arg1Of	supplier,described
R2290	T2918	T2916	arg2Of	supplier,by
R2291	T2918	T2917	arg1Of	supplier,the
R2292	T2920	T2919	arg1Of	microliter,One
R2293	T2920	T2921	arg1Of	microliter,of
R2294	T2920	T2923	arg1Of	microliter,was
R2295	T2920	T2924	arg2Of	microliter,used
R2296	T2922	T2921	arg2Of	cDNA,of
R2297	T2924	T2923	arg2Of	used,was
R2298	T2926	T2924	arg3Of	amplify,used
R2299	T2926	T2925	arg1Of	amplify,to
R2300	T2930	T2926	arg2Of	fragment,amplify
R2301	T2930	T2927	arg1Of	fragment,the
R2302	T2930	T2928	arg1Of	fragment,TLR4
R2303	T2930	T2929	arg1Of	fragment,gene
R2304	T2930	T2931	arg1Of	fragment,with
R2305	T2934	T2932	arg1Of	buffer,1×
R2306	T2934	T2933	arg1Of	buffer,PCR
R2307	T2934	T2935	arg1Of	buffer,","
R2308	T2935	T2937	arg1Of	",",","
R2309	T2936	T2935	arg2Of	1.5 mM MgCl2,","
R2310	T2937	T2942	arg1Of	",",","
R2311	T2938	T2937	arg2Of	200 μM,","
R2312	T2938	T2939	arg1Of	200 μM,of
R2313	T2941	T2939	arg2Of	dNTP,of
R2314	T2941	T2940	arg1Of	dNTP,each
R2315	T2942	T2947	arg1Of	",",and
R2316	T2943	T2942	arg2Of	200 μM,","
R2317	T2943	T2944	arg1Of	200 μM,of
R2318	T2945	T2944	arg2Of	primers,of
R2319	T2947	T2931	arg2Of	and,with
R2320	T2947	T2946	arg1Of	and,","
R2321	T2951	T2947	arg2Of	polymerase,and
R2322	T2951	T2948	arg1Of	polymerase,2u
R2323	T2951	T2949	arg1Of	polymerase,Taq
R2324	T2951	T2950	arg1Of	polymerase,DNA
R2325	T2955	T2952	arg1Of	gene,The
R2326	T2955	T2953	arg1Of	gene,house
R2327	T2955	T2954	arg1Of	gene,keep
R2328	T2955	T2956	arg1Of	gene,(
R2329	T2955	T2959	arg1Of	gene,was
R2330	T2955	T2960	arg2Of	gene,used
R2331	T2957	T2956	arg2Of	β-actin,(
R2332	T2958	T2956	arg3Of	),(
R2333	T2960	T2959	arg2Of	used,was
R2334	T2960	T2961	arg1Of	used,as
R2335	T2964	T2961	arg2Of	control,as
R2336	T2964	T2962	arg1Of	control,an
R2337	T2964	T2963	arg1Of	control,internal
R2338	T2968	T2965	arg1Of	sequences,The
R2339	T2968	T2966	arg1Of	sequences,PCR
R2340	T2968	T2967	arg1Of	sequences,primer
R2341	T2968	T2969	arg1Of	sequences,were
R2342	T2968	T2970	arg2Of	sequences,designed
R2343	T2970	T2969	arg2Of	designed,were
R2344	T2970	T2971	arg1Of	designed,according
R2345	T2972	T2971	arg2Of	to,according
R2346	T2974	T2975	arg1Of	TLR4,and
R2347	T2975	T2972	arg2Of	and,to
R2348	T2975	T2973	arg1Of	and,the
R2349	T2975	T2979	arg2Of	and,reported
R2350	T2978	T2975	arg2Of	sequences,and
R2351	T2978	T2976	arg1Of	sequences,β-actin
R2352	T2978	T2977	arg1Of	sequences,gene
R2353	T2979	T2980	arg1Of	reported,in
R2354	T2981	T2980	arg2Of	GenBank,in
R2355	T2982	T2983	arg1Of	TLR4,(
R2356	T2982	T2986	arg1Of	TLR4,:
R2357	T2982	T2987	arg1Of	TLR4,forward
R2358	T2982	T2988	arg1Of	TLR4,:
R2359	T2984	T2983	arg2Of	145 bp,(
R2360	T2985	T2983	arg3Of	),(
R2361	T2989	T2990	arg1Of	5′-AGCTTTGGTCAGTTGGCTCT-3′,;
R2362	T2990	T2992	arg1Of	;,:
R2363	T2991	T2990	arg2Of	reverse,;
R2364	T2993	T2994	arg1Of	5′-CAGGATGACACCATTGAAGC-3′,;
R2365	T2994	T2996	arg1Of	;,(
R2366	T2994	T2999	arg1Of	;,:
R2367	T2994	T3000	arg1Of	;,forward
R2368	T2994	T3001	arg1Of	;,:
R2369	T2995	T2994	arg2Of	β-actin,;
R2370	T2997	T2996	arg2Of	701 bp,(
R2371	T2998	T2996	arg3Of	),(
R2372	T3002	T3003	arg1Of	5′-GCCAACCGTGAAAAAGATG-3′,;
R2373	T3003	T3005	arg1Of	;,:
R2374	T3004	T3003	arg2Of	reverse,;
R2375	T3008	T3007	arg1Of	cycles,Reaction
R2376	T3008	T3009	arg1Of	cycles,were
R2377	T3008	T3010	arg2Of	cycles,performed
R2378	T3010	T3009	arg2Of	performed,were
R2379	T3010	T3011	arg1Of	performed,at
R2380	T3010	T3015	arg1Of	performed,:
R2381	T3014	T3011	arg2Of	condition,at
R2382	T3014	T3012	arg1Of	condition,the
R2383	T3014	T3013	arg1Of	condition,following
R2384	T3017	T3016	arg1Of	2 min,94°C
R2385	T3017	T3018	arg1Of	2 min,","
R2386	T3018	T3021	arg1Of	",",","
R2387	T3020	T3018	arg2Of	40 s,","
R2388	T3020	T3019	arg1Of	40 s,94°C
R2389	T3021	T3025	arg1Of	",",and
R2390	T3023	T3021	arg2Of	40 s,","
R2391	T3023	T3022	arg1Of	40 s,55°C
R2392	T3025	T3024	arg1Of	and,","
R2393	T3027	T3025	arg2Of	60 s,and
R2394	T3027	T3026	arg1Of	60 s,72°C
R2395	T3029	T3028	arg1Of	reaction,The
R2396	T3029	T3030	arg1Of	reaction,was
R2397	T3029	T3031	arg2Of	reaction,stopped
R2398	T3031	T3030	arg2Of	stopped,was
R2399	T3031	T3032	arg1Of	stopped,after
R2400	T3034	T3032	arg2Of	cycles,after
R2401	T3034	T3033	arg1Of	cycles,31
R2402	T3036	T3035	arg1Of	products,PCR
R2403	T3036	T3037	arg1Of	products,were
R2404	T3036	T3038	arg2Of	products,electrophoresed
R2405	T3038	T3037	arg2Of	electrophoresed,were
R2406	T3038	T3039	arg1Of	electrophoresed,through
R2407	T3041	T3039	arg2Of	gel,through
R2408	T3041	T3040	arg1Of	gel,agarose
R2409	T3043	T3042	arg1Of	Acquisition,Image
R2410	T3043	T3044	arg1Of	Acquisition,and
R2411	T3044	T3047	arg1Of	and,were
R2412	T3044	T3048	arg2Of	and,used
R2413	T3046	T3044	arg2Of	software,and
R2414	T3046	T3045	arg1Of	software,Analysis
R2415	T3048	T3047	arg2Of	used,were
R2416	T3048	T3049	modOf	used,to
R2417	T3050	T3049	arg1Of	determine,to
R2418	T3052	T3050	arg2Of	densities,determine
R2419	T3052	T3051	arg1Of	densities,band
R2420	T3054	T3053	arg1Of	abundance,The
R2421	T3054	T3055	arg1Of	abundance,of
R2422	T3054	T3058	arg1Of	abundance,was
R2423	T3054	T3059	arg2Of	abundance,expressed
R2424	T3057	T3055	arg2Of	mRNA,of
R2425	T3057	T3056	arg1Of	mRNA,TLR4
R2426	T3059	T3058	arg2Of	expressed,was
R2427	T3059	T3060	arg1Of	expressed,as
R2428	T3062	T3060	arg2Of	ratio,as
R2429	T3062	T3061	arg1Of	ratio,the
R2430	T3062	T3063	arg1Of	ratio,of
R2431	T3062	T3066	arg1Of	ratio,to
R2432	T3065	T3063	arg2Of	mRNA,of
R2433	T3065	T3064	arg1Of	mRNA,TLR4
R2434	T3068	T3066	arg2Of	mRNA,to
R2435	T3068	T3067	arg1Of	mRNA,β-actin
R2754	T3542	T3543	arg1Of	Immunohistochemistry,(
R2755	T3544	T3543	arg2Of	IHC,(
R2756	T3545	T3543	arg3Of	),(
R2757	T3548	T3546	arg1Of	staining,The
R2758	T3548	T3547	arg1Of	staining,IHC
R2759	T3548	T3549	arg1Of	staining,for
R2760	T3548	T3551	arg1Of	staining,was
R2761	T3548	T3552	arg2Of	staining,performed
R2762	T3550	T3549	arg2Of	TLR4,for
R2763	T3552	T3551	arg2Of	performed,was
R2764	T3552	T3553	arg1Of	performed,as
R2765	T3552	T3556	arg1Of	performed,[
R2766	T3554	T3553	arg2Of	described,as
R2767	T3554	T3555	arg1Of	described,previously
R2768	T3557	T3556	arg2Of	8,[
R2769	T3558	T3556	arg3Of	],[
R2770	T3561	T3559	arg1Of	antibody,The
R2771	T3561	T3560	arg1Of	antibody,primary
R2772	T3561	T3562	arg2Of	antibody,employed
R2773	T3561	T3563	arg1Of	antibody,was
R2774	T3563	T3566	arg1Of	was,(
R2775	T3565	T3563	arg2Of	anti-TLR4,was
R2776	T3565	T3564	arg1Of	anti-TLR4,rabbit
R2777	T3567	T3566	arg2Of	WuHan,(
R2778	T3567	T3568	arg1Of	WuHan,","
R2779	T3567	T3569	arg1Of	WuHan,BA1717
R2780	T3570	T3566	arg3Of	),(
R2781	T3574	T3571	arg1Of	antibody,The
R2782	T3574	T3572	arg2Of	antibody,biotinylated
R2783	T3574	T3573	arg1Of	antibody,secondary
R2784	T3574	T3575	arg1Of	antibody,and
R2785	T3575	T3584	arg1Of	and,were
R2786	T3575	T3585	arg2Of	and,obtained
R2787	T3577	T3575	arg2Of	complexes,and
R2788	T3577	T3576	arg1Of	complexes,antibody-biotin-avidin-peroxidase
R2789	T3577	T3578	arg1Of	complexes,(
R2790	T3580	T3578	arg2Of	reagent,(
R2791	T3580	T3579	arg1Of	reagent,ABC
R2792	T3580	T3581	arg1Of	reagent,","
R2793	T3582	T3581	arg2Of	SC-2018,","
R2794	T3583	T3578	arg3Of	),(
R2795	T3585	T3584	arg2Of	obtained,were
R2796	T3585	T3586	arg1Of	obtained,from
R2797	T3590	T3586	arg2Of	Inc.,from
R2798	T3590	T3587	arg1Of	Inc.,Santa
R2799	T3590	T3588	arg1Of	Inc.,Cruz
R2800	T3590	T3589	arg1Of	Inc.,Biotechnology
R2801	T3590	T3591	arg1Of	Inc.,(
R2802	T3593	T3591	arg2Of	Cruz,(
R2803	T3593	T3592	arg1Of	Cruz,Santa
R2804	T3593	T3594	arg1Of	Cruz,","
R2805	T3595	T3594	arg2Of	CA,","
R2806	T3596	T3591	arg3Of	),(
R2807	T3597	T3598	arg2Of	Slides,processed
R2808	T3598	T3599	arg1Of	processed,without
R2809	T3601	T3599	arg2Of	antibodies,without
R2810	T3601	T3600	arg1Of	antibodies,primary
R2811	T3601	T3602	arg2Of	antibodies,served
R2812	T3602	T3603	arg1Of	served,as
R2813	T3605	T3603	arg2Of	controls,as
R2814	T3605	T3604	arg1Of	controls,negative
R2889	T3746	T3745	arg1Of	Blots,Western
R2890	T3748	T3747	arg1Of	proteins,Cellular
R2891	T3748	T3749	arg1Of	proteins,were
R2892	T3748	T3750	arg2Of	proteins,prepared
R2893	T3750	T3749	arg2Of	prepared,were
R2894	T3750	T3751	arg1Of	prepared,from
R2895	T3754	T3751	arg2Of	hemispheres,from
R2896	T3754	T3752	arg1Of	hemispheres,ICH
R2897	T3754	T3753	arg1Of	hemispheres,cerebral
R2898	T3754	T3755	arg1Of	hemispheres,","
R2899	T3754	T3756	arg2Of	hemispheres,electrophoresed
R2900	T3754	T3761	arg2Of	hemispheres,transferred
R2901	T3754	T3766	arg1Of	hemispheres,(
R2902	T3756	T3757	arg1Of	electrophoresed,with
R2903	T3756	T3760	arg1Of	electrophoresed,and
R2904	T3759	T3757	arg2Of	gel,with
R2905	T3759	T3758	arg1Of	gel,SDS-polyacrylamide
R2906	T3761	T3760	arg2Of	transferred,and
R2907	T3761	T3762	arg1Of	transferred,onto
R2908	T3765	T3762	arg2Of	membranes,onto
R2909	T3765	T3763	arg1Of	membranes,Hybond
R2910	T3765	T3764	arg1Of	membranes,ECL
R2911	T3768	T3766	arg2Of	Pharmacia,(
R2912	T3768	T3767	arg1Of	Pharmacia,Amersham
R2913	T3768	T3769	arg1Of	Pharmacia,","
R2914	T3770	T3769	arg2Of	Piscataway,","
R2915	T3770	T3771	arg1Of	Piscataway,","
R2916	T3772	T3771	arg2Of	NJ,","
R2917	T3773	T3766	arg3Of	),(
R2918	T3776	T3774	arg1Of	membranes,The
R2919	T3776	T3775	arg1Of	membranes,ECL
R2920	T3776	T3777	arg1Of	membranes,were
R2921	T3776	T3778	arg2Of	membranes,incubated
R2922	T3778	T3777	arg2Of	incubated,were
R2923	T3778	T3779	arg1Of	incubated,with
R2924	T3783	T3779	arg2Of	antibody,with
R2925	T3783	T3780	arg1Of	antibody,the
R2926	T3783	T3781	arg1Of	antibody,appropriate
R2927	T3783	T3782	arg1Of	antibody,primary
R2928	T3783	T3784	arg1Of	antibody,(
R2929	T3783	T3789	arg2Of	antibody,followed
R2930	T3785	T3784	arg2Of	WuHan,(
R2931	T3785	T3786	arg1Of	WuHan,","
R2932	T3787	T3786	arg2Of	BA1717,","
R2933	T3788	T3784	arg3Of	),(
R2934	T3791	T3789	arg1Of	incubation,followed
R2935	T3791	T3790	arg2Of	incubation,by
R2936	T3791	T3792	arg1Of	incubation,with
R2937	T3795	T3792	arg2Of	antibodies,with
R2938	T3795	T3793	arg1Of	antibodies,peroxidase-conjugated
R2939	T3795	T3794	arg1Of	antibodies,secondary
R2940	T3795	T3796	arg1Of	antibodies,(
R2941	T3798	T3796	arg2Of	Bridge,(
R2942	T3798	T3797	arg1Of	Bridge,Golden
R2943	T3798	T3799	arg1Of	Bridge,","
R2944	T3800	T3799	arg2Of	PV-6001,","
R2945	T3801	T3796	arg3Of	),(
R2946	T3803	T3802	arg1Of	signals,The
R2947	T3803	T3804	arg1Of	signals,were
R2948	T3803	T3805	arg2Of	signals,detected
R2949	T3805	T3804	arg2Of	detected,were
R2950	T3805	T3806	arg1Of	detected,with
R2951	T3809	T3806	arg2Of	system,with
R2952	T3809	T3807	arg1Of	system,the
R2953	T3809	T3808	arg1Of	system,ECL
R2954	T3809	T3810	arg1Of	system,(
R2955	T3812	T3810	arg2Of	Pharmacia,(
R2956	T3812	T3811	arg1Of	Pharmacia,Amersham
R2957	T3813	T3810	arg3Of	),(
R2958	T3816	T3814	arg1Of	membranes,The
R2959	T3816	T3815	arg1Of	membranes,same
R2960	T3816	T3817	arg1Of	membranes,were
R2961	T3816	T3818	arg2Of	membranes,probed
R2962	T3816	T3832	arg1Of	membranes,being
R2963	T3816	T3833	arg2Of	membranes,washed
R2964	T3818	T3817	arg2Of	probed,were
R2965	T3818	T3819	arg1Of	probed,with
R2966	T3818	T3831	arg1Of	probed,after
R2967	T3820	T3819	arg2Of	anti-GAPDH,with
R2968	T3820	T3821	arg1Of	anti-GAPDH,(
R2969	T3823	T3821	arg2Of	dehydrogenase,(
R2970	T3823	T3822	arg1Of	dehydrogenase,glyceraldehyde-3-phosphate
R2971	T3823	T3824	arg1Of	dehydrogenase,","
R2972	T3823	T3828	arg1Of	dehydrogenase,","
R2973	T3823	T3829	arg1Of	dehydrogenase,Maine
R2974	T3825	T3826	arg1Of	Biodesign,","
R2975	T3826	T3824	arg2Of	",",","
R2976	T3827	T3826	arg2Of	Saco,","
R2977	T3830	T3821	arg3Of	),(
R2978	T3833	T3831	arg2Of	washed,after
R2979	T3833	T3832	arg2Of	washed,being
R2980	T3833	T3834	arg1Of	washed,with
R2981	T3835	T3834	arg2Of	stripping,with
R2982	T3836	T3835	arg2Of	buffer,stripping
R2983	T3838	T3837	arg1Of	signals,The
R2984	T3838	T3839	arg1Of	signals,were
R2985	T3838	T3840	arg2Of	signals,quantified
R2986	T3840	T3839	arg2Of	quantified,were
R2987	T3840	T3841	arg1Of	quantified,by
R2988	T3842	T3841	arg2Of	scanning,by
R2989	T3843	T3844	arg1Of	densitometry,and
R2990	T3844	T3842	arg2Of	and,scanning
R2991	T3847	T3844	arg2Of	analysis,and
R2992	T3847	T3845	arg1Of	analysis,computer-assisted
R2993	T3847	T3846	arg1Of	analysis,image
R3109	T4038	T4035	arg1Of	Assay,Electrophoretic
R3110	T4038	T4036	arg1Of	Assay,Mobility
R3111	T4038	T4037	arg1Of	Assay,Shift
R3112	T4038	T4039	arg1Of	Assay,(
R3113	T4040	T4039	arg2Of	EMSA,(
R3114	T4041	T4039	arg3Of	),(
R3115	T4043	T4042	arg1Of	proteins,Nuclear
R3116	T4043	T4044	arg1Of	proteins,were
R3117	T4043	T4045	arg2Of	proteins,isolated
R3118	T4045	T4044	arg2Of	isolated,were
R3119	T4045	T4046	arg1Of	isolated,from
R3120	T4048	T4046	arg2Of	tissue,from
R3121	T4048	T4047	arg1Of	tissue,brain
R3122	T4052	T4051	arg2Of	removal,after
R3123	T4052	T4053	arg1Of	removal,of
R3124	T4054	T4053	arg2Of	supernatant,of
R3125	T4054	T4055	arg1Of	supernatant,(
R3126	T4057	T4055	arg2Of	extract,(
R3127	T4057	T4056	arg1Of	extract,cytoplasmic
R3128	T4058	T4055	arg3Of	),(
R3129	T4061	T4060	arg1Of	pellets,the
R3130	T4061	T4062	arg1Of	pellets,were
R3131	T4061	T4063	arg2Of	pellets,resuspended
R3132	T4063	T4049	arg1Of	resuspended,Briefly
R3133	T4063	T4050	arg1Of	resuspended,","
R3134	T4063	T4051	arg1Of	resuspended,after
R3135	T4063	T4059	arg1Of	resuspended,","
R3136	T4063	T4062	arg2Of	resuspended,were
R3137	T4063	T4064	arg1Of	resuspended,in
R3138	T4067	T4064	arg2Of	reagent,in
R3139	T4067	T4065	arg1Of	reagent,nuclear
R3140	T4067	T4066	arg1Of	reagent,extraction
R3141	T4069	T4068	arg1Of	tubes,The
R3142	T4069	T4070	arg1Of	tubes,containing
R3143	T4069	T4072	arg1Of	tubes,were
R3144	T4069	T4073	arg2Of	tubes,put
R3145	T4071	T4070	arg2Of	pellets,containing
R3146	T4073	T4072	arg2Of	put,were
R3147	T4073	T4074	arg1Of	put,on
R3148	T4073	T4076	arg1Of	put,for
R3149	T4073	T4081	arg1Of	put,minutes
R3150	T4073	T4082	arg1Of	put,","
R3151	T4073	T4083	arg1Of	put,for
R3152	T4075	T4074	arg2Of	vortex,on
R3153	T4078	T4076	arg2Of	seconds,for
R3154	T4078	T4077	arg1Of	seconds,15
R3155	T4081	T4079	arg1Of	minutes,every
R3156	T4081	T4080	arg1Of	minutes,10
R3157	T4085	T4083	arg2Of	total,for
R3158	T4085	T4084	arg1Of	total,a
R3159	T4085	T4086	arg1Of	total,of
R3160	T4088	T4086	arg2Of	minutes,of
R3161	T4088	T4087	arg1Of	minutes,40
R3162	T4090	T4089	arg1Of	tubes,The
R3163	T4090	T4091	arg1Of	tubes,were
R3164	T4090	T4092	arg2Of	tubes,centrifuged
R3165	T4092	T4091	arg2Of	centrifuged,were
R3166	T4092	T4093	arg1Of	centrifuged,at
R3167	T4095	T4093	arg2Of	speed,at
R3168	T4095	T4094	arg1Of	speed,maximum
R3169	T4095	T4096	arg1Of	speed,(
R3170	T4095	T4100	arg1Of	speed,in
R3171	T4098	T4096	arg2Of	× g,(
R3172	T4098	T4097	arg1Of	× g,"~16,000"
R3173	T4099	T4096	arg3Of	),(
R3174	T4103	T4100	arg2Of	centrifuge,in
R3175	T4103	T4101	arg1Of	centrifuge,a
R3176	T4103	T4102	arg1Of	centrifuge,micro
R3177	T4103	T4104	arg1Of	centrifuge,for
R3178	T4106	T4104	arg2Of	minutes,for
R3179	T4106	T4105	arg1Of	minutes,10
R3180	T4108	T4107	arg1Of	supernatants,The
R3181	T4108	T4109	arg1Of	supernatants,(
R3182	T4108	T4113	arg1Of	supernatants,were
R3183	T4108	T4114	arg2Of	supernatants,transferred
R3184	T4108	T4121	arg2Of	supernatants,stored
R3185	T4111	T4109	arg2Of	extract,(
R3186	T4111	T4110	arg1Of	extract,nuclear
R3187	T4112	T4109	arg3Of	),(
R3188	T4114	T4115	arg1Of	transferred,to
R3189	T4114	T4120	arg1Of	transferred,and
R3190	T4119	T4115	arg2Of	tube,to
R3191	T4119	T4116	arg1Of	tube,a
R3192	T4119	T4117	arg1Of	tube,clean
R3193	T4119	T4118	arg1Of	tube,prechilled
R3194	T4120	T4113	arg2Of	and,were
R3195	T4121	T4120	arg2Of	stored,and
R3196	T4121	T4122	arg1Of	stored,at
R3197	T4121	T4124	arg1Of	stored,until
R3198	T4123	T4122	arg2Of	−80°C,at
R3199	T4125	T4124	arg2Of	use,until
R3200	T4126	T4127	arg1Of	EMSA,was
R3201	T4126	T4128	arg2Of	EMSA,performed
R3202	T4128	T4127	arg2Of	performed,was
R3203	T4128	T4129	arg1Of	performed,as
R3204	T4128	T4132	arg1Of	performed,[
R3205	T4130	T4129	arg2Of	described,as
R3206	T4130	T4131	arg1Of	described,previously
R3207	T4133	T4132	arg2Of	8,[
R3208	T4134	T4132	arg3Of	],[
R3209	T4137	T4135	arg1Of	activity,NFκB
R3210	T4137	T4136	arg1Of	activity,binding
R3211	T4137	T4138	arg1Of	activity,was
R3212	T4137	T4139	arg2Of	activity,examined
R3213	T4139	T4138	arg2Of	examined,was
R3214	T4139	T4140	arg1Of	examined,in
R3215	T4145	T4140	arg2Of	mixture,in
R3216	T4145	T4141	arg1Of	mixture,a
R3217	T4145	T4142	arg1Of	mixture,15 μL
R3218	T4145	T4143	arg1Of	mixture,binding
R3219	T4145	T4144	arg1Of	mixture,reaction
R3220	T4145	T4146	arg1Of	mixture,containing
R3221	T4147	T4148	arg1Of	15 μg,of
R3222	T4147	T4151	arg1Of	15 μg,and
R3223	T4150	T4148	arg2Of	proteins,of
R3224	T4150	T4149	arg1Of	proteins,nuclear
R3225	T4151	T4146	arg2Of	and,containing
R3226	T4152	T4153	arg1Of	35 fmol,[
R3227	T4154	T4153	arg2Of	γ-32P,[
R3228	T4155	T4153	arg3Of	],[
R3229	T4160	T4151	arg2Of	oligonucleotide,and
R3230	T4160	T4152	arg1Of	oligonucleotide,35 fmol
R3231	T4160	T4156	arg2Of	oligonucleotide,labeled
R3232	T4160	T4157	arg1Of	oligonucleotide,double-stranded
R3233	T4160	T4158	arg1Of	oligonucleotide,NFκB
R3234	T4160	T4159	arg1Of	oligonucleotide,consensus
R3235	T4164	T4161	arg1Of	assay,A
R3236	T4164	T4162	arg1Of	assay,super
R3237	T4164	T4163	arg1Of	assay,shift
R3238	T4164	T4165	arg1Of	assay,using
R3239	T4164	T4171	arg1Of	assay,was
R3240	T4164	T4172	arg2Of	assay,performed
R3241	T4166	T4165	arg2Of	antibodies,using
R3242	T4166	T4167	arg1Of	antibodies,to
R3243	T4168	T4169	arg1Of	P65,and
R3244	T4169	T4167	arg2Of	and,to
R3245	T4170	T4169	arg2Of	P50,and
R3246	T4172	T4171	arg2Of	performed,was
R3247	T4172	T4173	modOf	performed,to
R3248	T4174	T4173	arg1Of	confirm,to
R3249	T4177	T4174	arg2Of	specificity,confirm
R3250	T4177	T4175	arg1Of	specificity,NFκB
R3251	T4177	T4176	arg1Of	specificity,binding
R3252	T4177	T4178	arg1Of	specificity,as
R3253	T4180	T4178	arg2Of	described,as
R3254	T4180	T4179	arg1Of	described,previously
R3420	T4387	T4386	arg1Of	Analysis,Statistical
R3421	T4389	T4388	arg1Of	data,All
R3422	T4389	T4390	arg1Of	data,were
R3423	T4389	T4391	arg2Of	data,presented
R3424	T4391	T4390	arg2Of	presented,were
R3425	T4391	T4392	arg1Of	presented,as
R3426	T4395	T4392	arg2Of	SEM,as
R3427	T4395	T4393	arg1Of	SEM,mean
R3428	T4395	T4394	arg1Of	SEM,±
R3429	T4397	T4396	arg1Of	measurements,The
R3430	T4397	T4398	arg1Of	measurements,were
R3431	T4397	T4399	arg2Of	measurements,analyzed
R3432	T4399	T4398	arg2Of	analyzed,were
R3433	T4402	T4399	arg1Of	analysis,analyzed
R3434	T4402	T4400	arg2Of	analysis,by
R3435	T4402	T4401	arg1Of	analysis,one-way
R3436	T4402	T4403	arg1Of	analysis,of
R3437	T4404	T4405	arg1Of	variance,(
R3438	T4404	T4408	arg1Of	variance,or
R3439	T4406	T4405	arg2Of	ANOVA,(
R3440	T4407	T4405	arg3Of	),(
R3441	T4408	T4403	arg2Of	or,of
R3442	T4409	T4410	arg2Of	Student,'s
R3443	T4411	T4408	arg2Of	t-test,or
R3444	T4411	T4410	arg1Of	t-test,'s
R3445	T4414	T4415	arg1Of	<,.05
R3446	T4416	T4412	arg1Of	level,The
R3447	T4416	T4413	arg1Of	level,P
R3448	T4416	T4414	arg1Of	level,<
R3449	T4416	T4417	arg1Of	level,of
R3450	T4416	T4419	arg1Of	level,was
R3451	T4416	T4420	arg2Of	level,used
R3452	T4418	T4417	arg2Of	probability,of
R3453	T4420	T4419	arg2Of	used,was
R3454	T4420	T4421	arg1Of	used,as
R3455	T4423	T4421	arg2Of	criteria,as
R3456	T4423	T4422	arg1Of	criteria,the
R3457	T4423	T4424	arg1Of	criteria,of
R3458	T4425	T4424	arg2Of	significance,of

bionlp-st-ge-2016-test-proteins

Id	Subject	Object	Predicate	Lexical cue
T3726	4727-4767	Protein	denotes	glyceraldehyde-3-phosphate dehydrogenase
T3725	4720-4725	Protein	denotes	GAPDH
T2770	3736-3740	Protein	denotes	TLR4
T2769	3345-3352	Protein	denotes	β-actin
T2768	3256-3260	Protein	denotes	TLR4
T2767	3212-3219	Protein	denotes	β-actin
T2766	3203-3207	Protein	denotes	TLR4
T2765	3105-3112	Protein	denotes	β-actin
T2764	3084-3103	Protein	denotes	The house keep gene
T2763	3064-3082	Protein	denotes	Taq DNA polymerase
T2762	2964-2968	Protein	denotes	TLR4
T2761	2304-2308	Protein	denotes	TLR4
T4019	5741-5744	Protein	denotes	P50
T4018	5733-5736	Protein	denotes	P65
T3521	4029-4035	Protein	denotes	avidin
T3520	3857-3861	Protein	denotes	TLR4
T2772	3789-3796	Protein	denotes	β-actin
T2771	3776-3780	Protein	denotes	TLR4

bionlp-st-ge-2016-uniprot

Id	Subject	Object	Predicate	Lexical cue
T3959	4742-4767	http://www.uniprot.org/uniprot/P04406	denotes	3-phosphate dehydrogenase
T3958	4720-4725	http://www.uniprot.org/uniprot/P04406	denotes	GAPDH
T3676	3857-3861	http://www.uniprot.org/uniprot/O00206	denotes	TLR4
T3383	3789-3796	http://www.uniprot.org/uniprot/P60709	denotes	β-actin
T3382	3345-3352	http://www.uniprot.org/uniprot/P60709	denotes	β-actin
T3381	3212-3219	http://www.uniprot.org/uniprot/P60709	denotes	β-actin
T3380	3105-3112	http://www.uniprot.org/uniprot/P60709	denotes	β-actin
T3379	3776-3780	http://www.uniprot.org/uniprot/O00206	denotes	TLR4
T3378	3736-3740	http://www.uniprot.org/uniprot/O00206	denotes	TLR4
T3377	3256-3260	http://www.uniprot.org/uniprot/O00206	denotes	TLR4
T3376	3203-3207	http://www.uniprot.org/uniprot/O00206	denotes	TLR4
T3375	2964-2968	http://www.uniprot.org/uniprot/O00206	denotes	TLR4
T3374	2304-2308	http://www.uniprot.org/uniprot/O00206	denotes	TLR4
T3677	3951-3955	http://www.uniprot.org/uniprot/O00206	denotes	TLR4

UBERON-AE

Id	Subject	Object	Predicate	Lexical cue
T4005	5425-5429	http://purl.obolibrary.org/obo/UBERON_0000025	denotes	tube
T4004	5259-5264	http://purl.obolibrary.org/obo/UBERON_0000025	denotes	tubes
T4003	5151-5156	http://purl.obolibrary.org/obo/UBERON_0000025	denotes	tubes
T4002	5018-5024	http://purl.obolibrary.org/obo/UBERON_0000479	denotes	tissue
T4001	5012-5017	http://purl.obolibrary.org/obo/UBERON_0000955	denotes	brain
T3715	4281-4301	http://purl.obolibrary.org/obo/UBERON_0001869	denotes	cerebral hemispheres
T2236	1960-1965	http://purl.obolibrary.org/obo/UBERON_0000178	denotes	blood
T2235	2201-2208	http://purl.obolibrary.org/obo/UBERON_4200215	denotes	sutures
T2234	1714-1720	http://purl.obolibrary.org/obo/UBERON_4200215	denotes	suture
T2233	1706-1720	http://purl.obolibrary.org/obo/UBERON_0002489	denotes	coronal suture
T2232	1688-1693	http://purl.obolibrary.org/obo/UBERON_0003129	denotes	skull
T2231	1589-1594	http://purl.obolibrary.org/obo/UBERON_0000403	denotes	scalp
T1764	1068-1074	http://purl.obolibrary.org/obo/UBERON_0000955	denotes	brains
T1763	857-863	http://purl.obolibrary.org/obo/UBERON_0000955	denotes	brains
T1762	597-602	http://purl.obolibrary.org/obo/UBERON_0000178	denotes	blood

GO-BP

Id	Subject	Object	Predicate	Lexical cue
T3522	4142-4144	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T2775	2834-2843	http://purl.obolibrary.org/obo/GO_0009058	denotes	Synthesis
T2774	2852-2854	http://purl.obolibrary.org/obo/GO_0003964	denotes	RT
T2773	2331-2333	http://purl.obolibrary.org/obo/GO_0003964	denotes	RT
T1773	1117-1119	http://purl.obolibrary.org/obo/GO_0003964	denotes	RT
T3729	4839-4846	http://purl.obolibrary.org/obo/GO_0023052	denotes	signals
T3728	4615-4622	http://purl.obolibrary.org/obo/GO_0023052	denotes	signals
T3727	4240-4248	http://purl.obolibrary.org/obo/GO_0007349	denotes	Cellular

GO-MF

Id	Subject	Object	Predicate	Lexical cue
T4023	5719-5729	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T4022	5775-5782	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T4021	5555-5562	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T4020	5514-5521	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T1774	1117-1119	http://purl.obolibrary.org/obo/GO_0003964	denotes	RT
T3731	4574-4584	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T3730	4489-4497	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T3527	4142-4144	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T3526	4180-4190	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T3525	4013-4021	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T3524	4000-4008	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T3523	3917-3925	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T2777	2852-2854	http://purl.obolibrary.org/obo/GO_0003964	denotes	RT
T2776	2331-2333	http://purl.obolibrary.org/obo/GO_0003964	denotes	RT

GO-CC

Id	Subject	Object	Predicate	Lexical cue
T4025	5719-5729	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T4024	5719-5729	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T3528	3917-3925	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T2251	1916-1923	http://purl.obolibrary.org/obo/GO_0031430	denotes	midline
T2250	1741-1748	http://purl.obolibrary.org/obo/GO_0031430	denotes	midline
T2249	1617-1624	http://purl.obolibrary.org/obo/GO_0031430	denotes	midline
T3738	4574-4584	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T3737	4489-4497	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T3736	4574-4584	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T3735	4489-4497	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T3734	4688-4697	http://purl.obolibrary.org/obo/GO_0016020	denotes	membranes
T3733	4435-4444	http://purl.obolibrary.org/obo/GO_0016020	denotes	membranes
T3732	4379-4388	http://purl.obolibrary.org/obo/GO_0016020	denotes	membranes
T3535	4180-4190	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T3534	4013-4021	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T3533	4000-4008	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T3532	3917-3925	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T3531	4180-4190	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T3530	4013-4021	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T3529	4000-4008	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody

sentences

Id	Subject	Object	Predicate	Lexical cue
T4015	5693-5819	Sentence	denotes	A super shift assay using antibodies to P65 and P50 was performed to confirm NFκB binding specificity as previously described.
T4014	5509-5692	Sentence	denotes	NFκB binding activity was examined in a 15 μL binding reaction mixture containing 15 μg of nuclear proteins and 35 fmol [γ-32P] labeled double-stranded NFκB consensus oligonucleotide.
T4013	5461-5508	Sentence	denotes	EMSA was performed as described previously [8].
T4012	5351-5460	Sentence	denotes	The supernatants (nuclear extract) were transferred to a clean prechilled tube and stored at −80°C until use.
T4011	5255-5350	Sentence	denotes	The tubes were centrifuged at maximum speed (~16,000 × g) in a micro centrifuge for 10 minutes.
T4010	5147-5254	Sentence	denotes	The tubes containing pellets were put on vortex for 15 seconds every 10 minutes, for a total of 40 minutes.
T4009	5026-5146	Sentence	denotes	Briefly, after removal of supernatant (cytoplasmic extract), the pellets were resuspended in nuclear extraction reagent.
T4008	4976-5025	Sentence	denotes	Nuclear proteins were isolated from brain tissue.
T4007	4932-4975	Sentence	denotes	Electrophoretic Mobility Shift Assay (EMSA)
T4006	4927-4931	Sentence	denotes	2.6.
T3517	4147-4219	Sentence	denotes	Slides processed without primary antibodies served as negative controls.
T3516	3973-4146	Sentence	denotes	The biotinylated secondary antibody and antibody-biotin-avidin-peroxidase complexes (ABC reagent, SC-2018) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA).
T3515	3905-3972	Sentence	denotes	The primary antibody employed was rabbit anti-TLR4 (WuHan, BA1717).
T3514	3836-3904	Sentence	denotes	The IHC staining for TLR4 was performed as described previously [8].
T3513	3809-3835	Sentence	denotes	Immunohistochemistry (IHC)
T3512	3804-3808	Sentence	denotes	2.4.
T2748	3719-3802	Sentence	denotes	The abundance of TLR4 mRNA was expressed as the ratio of TLR4 mRNA to β-actin mRNA.
T2747	3640-3718	Sentence	denotes	Image Acquisition and Analysis software were used to determine band densities.
T2746	3585-3639	Sentence	denotes	PCR products were electrophoresed through agarose gel.
T2745	3543-3584	Sentence	denotes	The reaction was stopped after 31 cycles.
T2744	3494-3542	Sentence	denotes	94°C 2 min, 94°C 40 s, 55°C 40 s, and 72°C 60 s.
T2743	3435-3493	Sentence	denotes	Reaction cycles were performed at the following condition:
T2742	3408-3434	Sentence	denotes	5′-CCAGGATAGAGCCACCAAT-3′.
T2741	3372-3407	Sentence	denotes	5′-GCCAACCGTGAAAAAGATG-3′; reverse:
T2740	3317-3371	Sentence	denotes	5′-CAGGATGACACCATTGAAGC-3′; β-actin (701 bp): forward:
T2739	3280-3316	Sentence	denotes	5′-AGCTTTGGTCAGTTGGCTCT-3′; reverse:
T1772	969-1142	Sentence	denotes	Five rats in each group at each time point were sacrificed by over dose injection of Ketamine. The brains were removed and stored at minus 80°C for RT-PCR and Western Blots.
T1771	853-968	Sentence	denotes	The brains were removed, postfixed, embedded in paraffin, and cut into sections (7 μm) as described previously [8].
T1770	695-852	Sentence	denotes	Five rats in each group at each time point were sacrificed by transcardially perfusion of normal saline followed by 4% buffered Para formaldehyde (pH = 7.4).
T1769	388-694	Sentence	denotes	The experimental protocol used for animals was approved by the Animal Care and Use Committee, China Medical University. The rats were subjected to intra-cerebral injection of normal saline or autologous whole blood and were sacrificed on 6 hours, 1 day, 3 days and 7 days after the injection, respectively.
T1768	64-387	Sentence	denotes	250 g to 300 g) were provided by Experimental Animal Center, China Medical University. The rats were assigned randomly to three groups: normal group (N, n = 10, without any treatment), normal saline control (S, n = 40, intra-cerebral injection of normal saline as control) and intra-cerebral hemorrhage group (ICH, n = 40).
T1767	25-63	Sentence	denotes	Ninety Wister rats (male, body weight:
T1766	17-24	Sentence	denotes	Animals
T1765	12-16	Sentence	denotes	2.1.
T2248	2210-2284	Sentence	denotes	For the normal saline control rats, only 75 μL normal saline was injected.
T2247	2039-2209	Sentence	denotes	After the completion of the infusion, the needle was withdrawn quickly, cyanoacrylate glue was placed around the burr hole, and the skin incision was closed with sutures.
T2246	1926-2038	Sentence	denotes	Then, a 75 μL of autologous whole blood was infused at a rate of 20 μL/min with the use of a microinfusion pump.
T2245	1857-1925	Sentence	denotes	0.2 mm anterior, 6.0 mm ventral, and 3.0 mm lateral to the midline).
T2244	1750-1856	Sentence	denotes	A blunt 26-gauge needle was inserted into the left basal ganglia under stereotactic guidance (coordinates:
T2243	1626-1749	Sentence	denotes	Using a sterile technique, a 1 mm burr hole was opened in the skull on the left coronal suture 3 mm lateral to the midline.
T2242	1531-1625	Sentence	denotes	The rats were positioned in a stereotactic frame, and the scalp was incised along the midline.
T2241	1415-1530	Sentence	denotes	The body (rectal) temperature was maintained at 37°C during the surgery using a feedback-controlled heating system.
T2240	1337-1414	Sentence	denotes	Rats were anesthetized with intra-peritoneal sodium pentobarbital (60 mg/kg).
T2239	1187-1336	Sentence	denotes	Since most intracerebral hemorrhages occur in the basal ganglia clinically, in the present study, rat model of ICH was induced in left basal ganglia.
T2238	1149-1186	Sentence	denotes	Induction of Intracerebral Hemorrhage
T2237	1144-1148	Sentence	denotes	2.2.
T2738	3256-3279	Sentence	denotes	TLR4 (145 bp): forward:
T2737	3147-3255	Sentence	denotes	The PCR primer sequences were designed according to the TLR4 and β-actin gene sequences reported in GenBank.
T2736	3084-3146	Sentence	denotes	The house keep gene (β-actin) was used as an internal control.
T2735	2917-3083	Sentence	denotes	One microliter of cDNA was used to amplify the TLR4 gene fragment with 1× PCR buffer, 1.5 mM MgCl2, 200 μM of each dNTP, 200 μM of primers, and 2u Taq DNA polymerase.
T2734	2740-2916	Sentence	denotes	RNA was reverse-transcribed into cDNA in 20 μL reaction system using Superscript First-Strand Synthesis Kit for RT-PCR (promega Inc) under conditions described by the supplier.
T2733	2680-2739	Sentence	denotes	Then, the concentrations of mRNA were adjusted to 1.0 g/mL.
T2732	2536-2679	Sentence	denotes	The quantity of total RNA was determined on a Nanodrop (ND-1000 Spectrophotometer) by measuring optical density at A260 and A280 nm wavelength.
T2731	2338-2535	Sentence	denotes	Briefly, total RNA samples were isolated from peri-hemorrhage regions in left basal ganglia using Simply P Total RNA isolation Kit (BioFlux, HZ, China) according to the manufacturer's instructions.
T2730	2291-2337	Sentence	denotes	Detection of TLR4 mRNA by the Method of RT-PCR
T2729	2286-2290	Sentence	denotes	2.3.
T4384	5978-6052	Sentence	denotes	The P < .05 level of probability was used as the criteria of significance.
T4383	5886-5977	Sentence	denotes	The measurements were analyzed by one-way analysis of variance (ANOVA) or Student's t-test.
T4382	5847-5885	Sentence	denotes	All data were presented as mean ± SEM.
T4381	5826-5846	Sentence	denotes	Statistical Analysis
T4380	5821-5825	Sentence	denotes	2.7.
T3722	4835-4925	Sentence	denotes	The signals were quantified by scanning densitometry and computer-assisted image analysis.
T3721	4679-4834	Sentence	denotes	The same membranes were probed with anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase, Biodesign, Saco, Maine) after being washed with stripping buffer.
T3720	4611-4678	Sentence	denotes	The signals were detected with the ECL system (Amersham Pharmacia).
T3719	4427-4610	Sentence	denotes	The ECL membranes were incubated with the appropriate primary antibody (WuHan, BA1717) followed by incubation with peroxidase-conjugated secondary antibodies (Golden Bridge, PV-6001).
T3718	4240-4426	Sentence	denotes	Cellular proteins were prepared from ICH cerebral hemispheres, electrophoresed with SDS-polyacrylamide gel and transferred onto Hybond ECL membranes (Amersham Pharmacia, Piscataway, NJ).
T3717	4226-4239	Sentence	denotes	Western Blots
T3716	4221-4225	Sentence	denotes	2.5.
T26	0-2	Sentence	denotes	2.
T27	3-10	Sentence	denotes	Methods
T28	12-16	Sentence	denotes	2.1.
T29	17-24	Sentence	denotes	Animals
T30	25-63	Sentence	denotes	Ninety Wister rats (male, body weight:
T31	64-150	Sentence	denotes	250 g to 300 g) were provided by Experimental Animal Center, China Medical University.
T32	151-387	Sentence	denotes	The rats were assigned randomly to three groups: normal group (N, n = 10, without any treatment), normal saline control (S, n = 40, intra-cerebral injection of normal saline as control) and intra-cerebral hemorrhage group (ICH, n = 40).
T33	388-507	Sentence	denotes	The experimental protocol used for animals was approved by the Animal Care and Use Committee, China Medical University.
T34	508-694	Sentence	denotes	The rats were subjected to intra-cerebral injection of normal saline or autologous whole blood and were sacrificed on 6 hours, 1 day, 3 days and 7 days after the injection, respectively.
T35	695-852	Sentence	denotes	Five rats in each group at each time point were sacrificed by transcardially perfusion of normal saline followed by 4% buffered Para formaldehyde (pH = 7.4).
T36	853-968	Sentence	denotes	The brains were removed, postfixed, embedded in paraffin, and cut into sections (7 μm) as described previously [8].
T37	969-1063	Sentence	denotes	Five rats in each group at each time point were sacrificed by over dose injection of Ketamine.
T38	1064-1142	Sentence	denotes	The brains were removed and stored at minus 80°C for RT-PCR and Western Blots.
T39	1144-1148	Sentence	denotes	2.2.
T40	1149-1186	Sentence	denotes	Induction of Intracerebral Hemorrhage
T41	1187-1336	Sentence	denotes	Since most intracerebral hemorrhages occur in the basal ganglia clinically, in the present study, rat model of ICH was induced in left basal ganglia.
T42	1337-1414	Sentence	denotes	Rats were anesthetized with intra-peritoneal sodium pentobarbital (60 mg/kg).
T43	1415-1530	Sentence	denotes	The body (rectal) temperature was maintained at 37°C during the surgery using a feedback-controlled heating system.
T44	1531-1625	Sentence	denotes	The rats were positioned in a stereotactic frame, and the scalp was incised along the midline.
T45	1626-1749	Sentence	denotes	Using a sterile technique, a 1 mm burr hole was opened in the skull on the left coronal suture 3 mm lateral to the midline.
T46	1750-1856	Sentence	denotes	A blunt 26-gauge needle was inserted into the left basal ganglia under stereotactic guidance (coordinates:
T47	1857-1925	Sentence	denotes	0.2 mm anterior, 6.0 mm ventral, and 3.0 mm lateral to the midline).
T48	1926-2038	Sentence	denotes	Then, a 75 μL of autologous whole blood was infused at a rate of 20 μL/min with the use of a microinfusion pump.
T49	2039-2209	Sentence	denotes	After the completion of the infusion, the needle was withdrawn quickly, cyanoacrylate glue was placed around the burr hole, and the skin incision was closed with sutures.
T50	2210-2284	Sentence	denotes	For the normal saline control rats, only 75 μL normal saline was injected.
T51	2286-2290	Sentence	denotes	2.3.
T52	2291-2337	Sentence	denotes	Detection of TLR4 mRNA by the Method of RT-PCR
T53	2338-2535	Sentence	denotes	Briefly, total RNA samples were isolated from peri-hemorrhage regions in left basal ganglia using Simply P Total RNA isolation Kit (BioFlux, HZ, China) according to the manufacturer's instructions.
T54	2536-2679	Sentence	denotes	The quantity of total RNA was determined on a Nanodrop (ND-1000 Spectrophotometer) by measuring optical density at A260 and A280 nm wavelength.
T55	2680-2739	Sentence	denotes	Then, the concentrations of mRNA were adjusted to 1.0 g/mL.
T56	2740-2916	Sentence	denotes	RNA was reverse-transcribed into cDNA in 20 μL reaction system using Superscript First-Strand Synthesis Kit for RT-PCR (promega Inc) under conditions described by the supplier.
T57	2917-3083	Sentence	denotes	One microliter of cDNA was used to amplify the TLR4 gene fragment with 1× PCR buffer, 1.5 mM MgCl2, 200 μM of each dNTP, 200 μM of primers, and 2u Taq DNA polymerase.
T58	3084-3146	Sentence	denotes	The house keep gene (β-actin) was used as an internal control.
T59	3147-3255	Sentence	denotes	The PCR primer sequences were designed according to the TLR4 and β-actin gene sequences reported in GenBank.
T60	3256-3279	Sentence	denotes	TLR4 (145 bp): forward:
T61	3280-3316	Sentence	denotes	5′-AGCTTTGGTCAGTTGGCTCT-3′; reverse:
T62	3317-3371	Sentence	denotes	5′-CAGGATGACACCATTGAAGC-3′; β-actin (701 bp): forward:
T63	3372-3407	Sentence	denotes	5′-GCCAACCGTGAAAAAGATG-3′; reverse:
T64	3408-3434	Sentence	denotes	5′-CCAGGATAGAGCCACCAAT-3′.
T65	3435-3493	Sentence	denotes	Reaction cycles were performed at the following condition:
T66	3494-3542	Sentence	denotes	94°C 2 min, 94°C 40 s, 55°C 40 s, and 72°C 60 s.
T67	3543-3584	Sentence	denotes	The reaction was stopped after 31 cycles.
T68	3585-3639	Sentence	denotes	PCR products were electrophoresed through agarose gel.
T69	3640-3718	Sentence	denotes	Image Acquisition and Analysis software were used to determine band densities.
T70	3719-3802	Sentence	denotes	The abundance of TLR4 mRNA was expressed as the ratio of TLR4 mRNA to β-actin mRNA.
T71	3804-3808	Sentence	denotes	2.4.
T72	3809-3835	Sentence	denotes	Immunohistochemistry (IHC)
T73	3836-3904	Sentence	denotes	The IHC staining for TLR4 was performed as described previously [8].
T74	3905-3972	Sentence	denotes	The primary antibody employed was rabbit anti-TLR4 (WuHan, BA1717).
T75	3973-4146	Sentence	denotes	The biotinylated secondary antibody and antibody-biotin-avidin-peroxidase complexes (ABC reagent, SC-2018) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA).
T76	4147-4219	Sentence	denotes	Slides processed without primary antibodies served as negative controls.
T77	4221-4225	Sentence	denotes	2.5.
T78	4226-4239	Sentence	denotes	Western Blots
T79	4240-4426	Sentence	denotes	Cellular proteins were prepared from ICH cerebral hemispheres, electrophoresed with SDS-polyacrylamide gel and transferred onto Hybond ECL membranes (Amersham Pharmacia, Piscataway, NJ).
T80	4427-4610	Sentence	denotes	The ECL membranes were incubated with the appropriate primary antibody (WuHan, BA1717) followed by incubation with peroxidase-conjugated secondary antibodies (Golden Bridge, PV-6001).
T81	4611-4678	Sentence	denotes	The signals were detected with the ECL system (Amersham Pharmacia).
T82	4679-4834	Sentence	denotes	The same membranes were probed with anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase, Biodesign, Saco, Maine) after being washed with stripping buffer.
T83	4835-4925	Sentence	denotes	The signals were quantified by scanning densitometry and computer-assisted image analysis.
T84	4927-4931	Sentence	denotes	2.6.
T85	4932-4975	Sentence	denotes	Electrophoretic Mobility Shift Assay (EMSA)
T86	4976-5025	Sentence	denotes	Nuclear proteins were isolated from brain tissue.
T87	5026-5146	Sentence	denotes	Briefly, after removal of supernatant (cytoplasmic extract), the pellets were resuspended in nuclear extraction reagent.
T88	5147-5254	Sentence	denotes	The tubes containing pellets were put on vortex for 15 seconds every 10 minutes, for a total of 40 minutes.
T89	5255-5350	Sentence	denotes	The tubes were centrifuged at maximum speed (~16,000 × g) in a micro centrifuge for 10 minutes.
T90	5351-5460	Sentence	denotes	The supernatants (nuclear extract) were transferred to a clean prechilled tube and stored at −80°C until use.
T91	5461-5508	Sentence	denotes	EMSA was performed as described previously [8].
T92	5509-5692	Sentence	denotes	NFκB binding activity was examined in a 15 μL binding reaction mixture containing 15 μg of nuclear proteins and 35 fmol [γ-32P] labeled double-stranded NFκB consensus oligonucleotide.
T93	5693-5819	Sentence	denotes	A super shift assay using antibodies to P65 and P50 was performed to confirm NFκB binding specificity as previously described.
T94	5821-5825	Sentence	denotes	2.7.
T95	5826-5846	Sentence	denotes	Statistical Analysis
T96	5847-5885	Sentence	denotes	All data were presented as mean ± SEM.
T97	5886-5977	Sentence	denotes	The measurements were analyzed by one-way analysis of variance (ANOVA) or Student's t-test.
T98	5978-6052	Sentence	denotes	The P < .05 level of probability was used as the criteria of significance.

simple1

Id	Subject	Object	Predicate	Lexical cue
T4033	5741-5744	Protein	denotes	P50
T4032	5733-5736	Protein	denotes	P65
T2815	3789-3796	Protein	denotes	β-actin
T2814	3776-3780	Protein	denotes	TLR4
T2813	3736-3740	Protein	denotes	TLR4
T2812	3345-3352	Protein	denotes	β-actin
T2811	3256-3260	Protein	denotes	TLR4
T2810	3212-3219	Protein	denotes	β-actin
T2809	3203-3207	Protein	denotes	TLR4
T2808	3105-3112	Protein	denotes	β-actin
T2807	3084-3103	Protein	denotes	The house keep gene
T2806	3064-3082	Protein	denotes	Taq DNA polymerase
T2805	2964-2968	Protein	denotes	TLR4
T2804	2304-2308	Protein	denotes	TLR4
T3743	4727-4767	Protein	denotes	glyceraldehyde-3-phosphate dehydrogenase
T3742	4720-4725	Protein	denotes	GAPDH
T3540	4029-4035	Protein	denotes	avidin
T3539	3857-3861	Protein	denotes	TLR4

BioNLP16_DUT

Id	Subject	Object	Predicate	Lexical cue
T4360	5741-5744	Protein	denotes	P50
T4359	5733-5736	Protein	denotes	P65
T3972	4727-4767	Protein	denotes	glyceraldehyde-3-phosphate dehydrogenase
T3971	4720-4725	Protein	denotes	GAPDH
T3690	4029-4035	Protein	denotes	avidin
T3689	3857-3861	Protein	denotes	TLR4
T3473	3723-3732	Transcription	denotes	abundance
T3472	3789-3796	Protein	denotes	β-actin
T3471	3776-3780	Protein	denotes	TLR4
T3470	3736-3740	Protein	denotes	TLR4
T3469	3345-3352	Protein	denotes	β-actin
T3468	3256-3260	Protein	denotes	TLR4
T3467	3212-3219	Protein	denotes	β-actin
T3466	3203-3207	Protein	denotes	TLR4
T3465	3105-3112	Protein	denotes	β-actin
T3464	3084-3103	Protein	denotes	The house keep gene
T3463	3064-3082	Protein	denotes	Taq DNA polymerase
T3462	2964-2968	Protein	denotes	TLR4
T3461	2304-2308	Protein	denotes	TLR4
R2753	T3470	T3473	themeOf	TLR4,abundance

BioNLP16_Messiy

Id	Subject	Object	Predicate	Lexical cue
T4346	5741-5744	Protein	denotes	P50
T4345	5733-5736	Protein	denotes	P65
T3961	4727-4767	Protein	denotes	glyceraldehyde-3-phosphate dehydrogenase
T3960	4720-4725	Protein	denotes	GAPDH
T3679	4029-4035	Protein	denotes	avidin
T3678	3857-3861	Protein	denotes	TLR4
T3396	3750-3759	Transcription	denotes	expressed
T3395	3789-3796	Protein	denotes	β-actin
T3394	3776-3780	Protein	denotes	TLR4
T3393	3736-3740	Protein	denotes	TLR4
T3392	3345-3352	Protein	denotes	β-actin
T3391	3256-3260	Protein	denotes	TLR4
T3390	3212-3219	Protein	denotes	β-actin
T3389	3203-3207	Protein	denotes	TLR4
T3388	3105-3112	Protein	denotes	β-actin
T3387	3084-3103	Protein	denotes	The house keep gene
T3386	3064-3082	Protein	denotes	Taq DNA polymerase
T3385	2964-2968	Protein	denotes	TLR4
T3384	2304-2308	Protein	denotes	TLR4
R2741	T3393	T3396	themeOf	TLR4,expressed

DLUT931

Id	Subject	Object	Predicate	Lexical cue
T4350	5741-5744	Protein	denotes	P50
T4349	5733-5736	Protein	denotes	P65
T3965	4727-4767	Protein	denotes	glyceraldehyde-3-phosphate dehydrogenase
T3964	4720-4725	Protein	denotes	GAPDH
T3685	4029-4035	Protein	denotes	avidin
T3684	3857-3861	Protein	denotes	TLR4
T3413	3781-3785	Transcription	denotes	mRNA
T3412	3741-3745	Transcription	denotes	mRNA
T3411	3723-3732	Transcription	denotes	abundance
T3410	2309-2313	Transcription	denotes	mRNA
T3409	2291-2300	Transcription	denotes	Detection
T3408	3789-3796	Protein	denotes	β-actin
T3407	3776-3780	Protein	denotes	TLR4
T3406	3736-3740	Protein	denotes	TLR4
T3405	3345-3352	Protein	denotes	β-actin
T3404	3256-3260	Protein	denotes	TLR4
T3403	3212-3219	Protein	denotes	β-actin
T3402	3203-3207	Protein	denotes	TLR4
T3401	3105-3112	Protein	denotes	β-actin
T3400	3084-3103	Protein	denotes	The house keep gene
T3399	3064-3082	Protein	denotes	Taq DNA polymerase
T3398	2964-2968	Protein	denotes	TLR4
T3397	2304-2308	Protein	denotes	TLR4
T3414	3797-3801	Transcription	denotes	mRNA
R2745	T3406	T3412	themeOf	TLR4,mRNA
R2746	T3407	T3413	themeOf	TLR4,mRNA
R2747	T3408	T3414	themeOf	β-actin,mRNA
R2742	T3397	T3409	themeOf	TLR4,Detection
R2743	T3397	T3410	themeOf	TLR4,mRNA
R2744	T3406	T3411	themeOf	TLR4,abundance

bionlp-st-ge-2016-test-ihmc

Id	Subject	Object	Predicate	Lexical cue
T3988	4240-4257	Protein	denotes	Cellular proteins
T3987	4679-4697	Entity	denotes	The same membranes
T3986	4646-4649	Entity	denotes	ECL
T3985	4542-4584	Entity	denotes	peroxidase-conjugated secondary antibodies
T3984	4431-4434	Protein	denotes	ECL
T3983	4368-4409	Entity	denotes	Hybond ECL membranes (Amersham Pharmacia,
T3982	4553-4584	Entity	denotes	conjugated secondary antibodies
T3981	4715-4725	Protein	denotes	anti-GAPDH
T3980	4375-4378	Protein	denotes	ECL
T3979	4328-4342	Entity	denotes	polyacrylamide
T3978	4542-4584	Protein	denotes	peroxidase-conjugated secondary antibodies
T3977	4324-4362	Entity	denotes	SDS-polyacrylamide gel and transferred
T3976	4465-4497	Protein	denotes	the appropriate primary antibody
T3975	4427-4444	Entity	denotes	The ECL membranes
T3974	4324-4327	Protein	denotes	SDS
T3973	4727-4779	Protein	denotes	glyceraldehyde-3-phosphate dehydrogenase, Biodesign,
T3704	3973-4008	Protein_modification	denotes	The biotinylated secondary antibody
T3703	4058-4079	Entity	denotes	ABC reagent, SC-2018)
T3702	4036-4046	Protein	denotes	peroxidase
T3701	4172-4190	Entity	denotes	primary antibodies
T3700	3857-3861	Protein	denotes	TLR4
T3699	3905-3934	Protein	denotes	The primary antibody employed
T3698	3939-3971	Protein	denotes	rabbit anti-TLR4 (WuHan, BA1717)
T3697	4013-4056	Entity	denotes	antibody-biotin-avidin-peroxidase complexes
T3696	3973-4008	Protein	denotes	The biotinylated secondary antibody
T3695	4147-4218	Entity	denotes	Slides processed without primary antibodies served as negative controls
T3694	4022-4028	Entity	denotes	biotin
T3693	4029-4035	Protein	denotes	avidin
T3692	4099-4104	Protein	denotes	Santa
T3691	4013-4021	Entity	denotes	antibody
T3491	3585-3588	Protein	denotes	PCR
T3490	3105-3112	Protein	denotes	β-actin
T3489	3247-3250	Protein	denotes	Gen
T3488	3084-3113	Protein	denotes	The house keep gene (β-actin)
T3487	3317-3352	Protein	denotes	5′-CAGGATGACACCATTGAAGC-3′; β-actin
T3486	2301-2313	Protein	denotes	of TLR4 mRNA
T3485	3256-3260	Protein	denotes	TLR4
T3484	2960-3002	Protein	denotes	the TLR4 gene fragment with 1× PCR buffer,
T3483	2353-2356	Protein	denotes	RNA
T3482	3151-3154	Protein	denotes	PCR
T3481	2991-2994	Protein	denotes	PCR
T3480	2964-2968	Protein	denotes	TLR4
T3479	2304-2308	Protein	denotes	TLR4
T3478	2651-2655	Entity	denotes	A260
T3477	2705-2712	Protein	denotes	of mRNA
T3476	2809-2847	Protein	denotes	Superscript First-Strand Synthesis Kit
T3475	2451-2454	Protein	denotes	RNA
T3474	3061-3082	Entity	denotes	2u Taq DNA polymerase
T2671	2214-2231	Entity	denotes	the normal saline
T3460	3776-3780	Protein	denotes	TLR4
T3459	3199-3207	Protein	denotes	the TLR4
T3458	3773-3785	Protein	denotes	of TLR4 mRNA
T3457	3733-3745	Protein	denotes	of TLR4 mRNA
T3456	2660-2665	Entity	denotes	A280
T3455	3786-3801	Protein	denotes	to β-actin mRNA
T3454	3736-3740	Protein	denotes	TLR4
T3453	3061-3082	Protein	denotes	2u Taq DNA polymerase
T3452	3627-3638	Entity	denotes	agarose gel
T3451	2960-3002	Protein	denotes	the TLR4 gene fragment with 1× PCR buffer,
T3450	3212-3254	Protein	denotes	β-actin gene sequences reported in GenBank
T3449	3033-3037	Entity	denotes	NTP,
T3448	3627-3634	Entity	denotes	agarose
T3447	2852-2872	Protein	denotes	RT-PCR (promega Inc)
T3446	3786-3801	Protein	denotes	to β-actin mRNA
T3445	2740-2743	Protein	denotes	RNA
T3444	2443-2468	Protein	denotes	P Total RNA isolation Kit
T3443	2549-2561	Protein	denotes	of total RNA
T3442	3247-3254	Protein	denotes	GenBank
T3441	2328-2337	Protein	denotes	of RT-PCR
T2228	823-827	Entity	denotes	Para
T2227	217-270	Entity	denotes	n = 10, without any treatment), normal saline control
T2226	563-576	Entity	denotes	normal saline
T2225	901-910	Entity	denotes	paraffin,
T2224	782-813	Entity	denotes	of normal saline followed by 4%
T2223	823-840	Entity	denotes	Para formaldehyde
T2222	272-278	Entity	denotes	S, n =
T2221	1117-1123	Protein	denotes	RT-PCR
T2220	1054-1062	Entity	denotes	Ketamine
T2219	379-382	Entity	denotes	n =
T2218	256-262	Entity	denotes	saline
T2678	1634-1641	Protein	denotes	sterile
T2677	1737-1748	Entity	denotes	the midline
T2676	2060-2075	Entity	denotes	of the infusion
T2675	1365-1413	Entity	denotes	intra-peritoneal sodium pentobarbital (60 mg/kg)
T2674	1613-1624	Entity	denotes	the midline
T2673	1912-1923	Entity	denotes	the midline
T2672	2246-2270	Entity	denotes	only 75 μL normal saline
T4473	5978-6010	Entity	denotes	The P < .05 level of probability
T4472	5950-5955	Protein	denotes	ANOVA
T4471	5970-5971	Entity	denotes	t
T4377	5741-5744	Protein	denotes	P50
T4376	5369-5384	Entity	denotes	nuclear extract
T4375	5255-5264	Protein	denotes	The tubes
T4374	5733-5736	Protein	denotes	P65
T4373	5645-5665	Protein	denotes	double-stranded NFκB
T4372	5147-5175	Protein	denotes	The tubes containing pellets
T4371	5563-5590	Entity	denotes	reaction mixture containing
T4370	5666-5691	Entity	denotes	consensus oligonucleotide
T4369	5600-5616	Protein	denotes	nuclear proteins
T4368	5351-5385	Entity	denotes	The supernatants (nuclear extract)
T4367	5119-5145	Entity	denotes	nuclear extraction reagent
T4366	5188-5194	Entity	denotes	vortex
T4365	4976-4992	Protein	denotes	Nuclear proteins
T4364	5049-5085	Entity	denotes	of supernatant (cytoplasmic extract)
T4363	5065-5084	Entity	denotes	cytoplasmic extract
T4362	5425-5459	Protein	denotes	tube and stored at −80°C until use
T4361	5719-5729	Entity	denotes	antibodies
R2885	T3696	T3704	themeOf	The biotinylated secondary antibody,The biotinylated secondary antibody

bionlp-st-ge-2016-spacy-parsed

Id	Subject	Object	Predicate	Lexical cue
T2666	2283-2284	.	denotes	.
T3088	2384-2399	JJ	denotes	peri-hemorrhage
T4470	6051-6052	.	denotes	.
T4469	6039-6051	NN	denotes	significance
T4468	6036-6038	IN	denotes	of
T4467	6027-6035	NNS	denotes	criteria
T4466	6023-6026	DT	denotes	the
T4465	6020-6022	IN	denotes	as
T4464	6015-6019	VBN	denotes	used
T4463	6011-6014	VBD	denotes	was
T4462	5999-6010	NN	denotes	probability
T4461	5996-5998	IN	denotes	of
T4460	5990-5995	NN	denotes	level
T4459	5986-5989	CD	denotes	.05
T4458	5984-5985	NN	denotes	<
T4457	5982-5983	NNP	denotes	P
T4456	5978-5981	DT	denotes	The
T4455	5976-5977	.	denotes	.
T4454	5970-5976	JJS	denotes	t-test
T4453	5967-5969	POS	denotes	's
T4452	5960-5967	NN	denotes	Student
T4451	5957-5959	CC	denotes	or
T4450	5955-5956	-RRB-	denotes	)
T4449	5950-5955	NNP	denotes	ANOVA
T4448	5949-5950	-LRB-	denotes	(
T4447	5940-5948	NN	denotes	variance
T4446	5937-5939	IN	denotes	of
T4445	5928-5936	NN	denotes	analysis
T4444	5920-5927	JJ	denotes	one-way
T4443	5917-5919	IN	denotes	by
T4442	5908-5916	VBN	denotes	analyzed
T4441	5903-5907	VBD	denotes	were
T4440	5890-5902	NNS	denotes	measurements
T4439	5886-5889	DT	denotes	The
T4438	5884-5885	.	denotes	.
T4437	5881-5884	NNP	denotes	SEM
T4436	5879-5880	NN	denotes	±
T4435	5874-5878	JJ	denotes	mean
T4434	5871-5873	IN	denotes	as
T4433	5861-5870	VBN	denotes	presented
T4432	5856-5860	VBD	denotes	were
T4431	5851-5855	NNS	denotes	data
T4430	5847-5850	NNP	denotes	All
T4429	5838-5846	NNP	denotes	Analysis
T4428	5826-5837	NNP	denotes	Statistical
T4427	5824-5825	.	denotes	.
T4426	5821-5824	CD	denotes	2.7
T4344	5818-5819	.	denotes	.
T4343	5809-5818	VBN	denotes	described
T4342	5798-5808	RB	denotes	previously
T4341	5795-5797	IN	denotes	as
T4340	5783-5794	NN	denotes	specificity
T4339	5775-5782	JJ	denotes	binding
T4338	5770-5774	NNP	denotes	NFκB
T4337	5762-5769	VB	denotes	confirm
T4336	5759-5761	TO	denotes	to
T4335	5749-5758	VBN	denotes	performed
T4334	5745-5748	VBD	denotes	was
T4333	5741-5744	NNP	denotes	P50
T4332	5737-5740	CC	denotes	and
T4331	5733-5736	NNP	denotes	P65
T4330	5730-5732	TO	denotes	to
T4329	5719-5729	NNS	denotes	antibodies
T4328	5713-5718	VBG	denotes	using
T4327	5707-5712	NN	denotes	assay
T4326	5701-5706	NN	denotes	shift
T4325	5695-5700	JJ	denotes	super
T4324	5693-5694	DT	denotes	A
T4323	5691-5692	.	denotes	.
T4322	5676-5691	NN	denotes	oligonucleotide
T4321	5666-5675	NN	denotes	consensus
T4320	5661-5665	NNP	denotes	NFκB
T4319	5645-5660	JJ	denotes	double-stranded
T4318	5637-5644	VBD	denotes	labeled
T4317	5635-5636	NNP	denotes	]
T4316	5630-5635	NNP	denotes	γ-32P
T4315	5629-5630	NNP	denotes	[
T4314	5624-5628	NN	denotes	fmol
T4313	5621-5623	CD	denotes	35
T4312	5617-5620	CC	denotes	and
T4311	5608-5616	NNS	denotes	proteins
T4310	5600-5607	JJ	denotes	nuclear
T4309	5597-5599	IN	denotes	of
T4308	5594-5596	NN	denotes	μg
T4307	5591-5593	CD	denotes	15
T4306	5580-5590	VBG	denotes	containing
T4305	5572-5579	NN	denotes	mixture
T4304	5563-5571	NN	denotes	reaction
T4303	5555-5562	JJ	denotes	binding
T4302	5552-5554	NN	denotes	μL
T4301	5549-5551	CD	denotes	15
T4300	5547-5548	DT	denotes	a
T4299	5544-5546	IN	denotes	in
T4298	5535-5543	VBN	denotes	examined
T4297	5531-5534	VBD	denotes	was
T4296	5522-5530	NN	denotes	activity
T4295	5514-5521	JJ	denotes	binding
T4294	5509-5513	NNP	denotes	NFκB
T4293	5507-5508	.	denotes	.
T4292	5506-5507	NN	denotes	]
T4291	5505-5506	CD	denotes	8
T4290	5504-5505	JJ	denotes	[
T4289	5493-5503	RB	denotes	previously
T4288	5483-5492	VBN	denotes	described
T4287	5480-5482	IN	denotes	as
T4286	5470-5479	VBN	denotes	performed
T4285	5466-5469	VBD	denotes	was
T4284	5461-5465	NNP	denotes	EMSA
T4283	5459-5460	.	denotes	.
T4282	5456-5459	NN	denotes	use
T4281	5450-5455	IN	denotes	until
T4280	5448-5449	NNP	denotes	C
T4279	5447-5448	CD	denotes	°
T4278	5445-5447	CD	denotes	80
T4277	5444-5445	CD	denotes	−
T4276	5441-5443	IN	denotes	at
T4275	5434-5440	VBD	denotes	stored
T4274	5430-5433	CC	denotes	and
T4273	5425-5429	NN	denotes	tube
T4272	5414-5424	JJ	denotes	prechilled
T4271	5408-5413	JJ	denotes	clean
T4270	5406-5407	DT	denotes	a
T4269	5403-5405	TO	denotes	to
T4268	5391-5402	VBN	denotes	transferred
T4267	5386-5390	VBD	denotes	were
T4266	5384-5385	-RRB-	denotes	)
T4265	5377-5384	VB	denotes	extract
T4264	5369-5376	JJ	denotes	nuclear
T4263	5368-5369	-LRB-	denotes	(
T4262	5355-5367	NNS	denotes	supernatants
T4261	5351-5354	DT	denotes	The
T4260	5349-5350	.	denotes	.
T4259	5342-5349	NNS	denotes	minutes
T4258	5339-5341	CD	denotes	10
T4257	5335-5338	IN	denotes	for
T4256	5324-5334	NN	denotes	centrifuge
T4255	5318-5323	NN	denotes	micro
T4254	5316-5317	DT	denotes	a
T4253	5313-5315	IN	denotes	in
T4252	5311-5312	-RRB-	denotes	)
T4251	5310-5311	NN	denotes	g
T4250	5308-5309	CD	denotes	×
T4249	5301-5307	CD	denotes	16,000
T4248	5300-5301	FW	denotes	~
T4247	5299-5300	-LRB-	denotes	(
T4246	5293-5298	NN	denotes	speed
T4245	5285-5292	JJ	denotes	maximum
T4244	5282-5284	IN	denotes	at
T4243	5270-5281	VBN	denotes	centrifuged
T4242	5265-5269	VBD	denotes	were
T4241	5259-5264	NNS	denotes	tubes
T4240	5255-5258	DT	denotes	The
T4218	5147-5150	DT	denotes	The
T4217	5145-5146	.	denotes	.
T4216	5138-5145	NN	denotes	reagent
T4215	5127-5137	NN	denotes	extraction
T4214	5119-5126	JJ	denotes	nuclear
T4213	5116-5118	IN	denotes	in
T4212	5104-5115	VBN	denotes	resuspended
T4211	5099-5103	VBD	denotes	were
T4210	5091-5098	NNS	denotes	pellets
T4209	5087-5090	DT	denotes	the
T4208	5085-5086	,	denotes	,
T4207	5084-5085	-RRB-	denotes	)
T4206	5077-5084	VB	denotes	extract
T4205	5065-5076	JJ	denotes	cytoplasmic
T4204	5064-5065	-LRB-	denotes	(
T4203	5052-5063	JJ	denotes	supernatant
T4202	5049-5051	IN	denotes	of
T4201	5041-5048	NN	denotes	removal
T4200	5035-5040	IN	denotes	after
T4199	5033-5034	,	denotes	,
T4198	5026-5033	RB	denotes	Briefly
T4197	5024-5025	.	denotes	.
T4196	5018-5024	NN	denotes	tissue
T4195	5012-5017	NN	denotes	brain
T4194	5007-5011	IN	denotes	from
T4193	4998-5006	VBN	denotes	isolated
T4192	4993-4997	VBD	denotes	were
T4191	4984-4992	NNS	denotes	proteins
T4190	4976-4983	NNP	denotes	Nuclear
T4189	4974-4975	-RRB-	denotes	)
T4188	4970-4974	NNP	denotes	EMSA
T4187	4969-4970	-LRB-	denotes	(
T4186	4963-4968	NNP	denotes	Assay
T4185	4957-4962	NNP	denotes	Shift
T4184	4948-4956	NNP	denotes	Mobility
T3957	4924-4925	.	denotes	.
T3956	4916-4924	NN	denotes	analysis
T3955	4910-4915	NN	denotes	image
T3954	4892-4909	JJ	denotes	computer-assisted
T3953	4888-4891	CC	denotes	and
T3952	4875-4887	NN	denotes	densitometry
T3951	4866-4874	VBG	denotes	scanning
T3950	4863-4865	IN	denotes	by
T3949	4852-4862	VBN	denotes	quantified
T3948	4847-4851	VBD	denotes	were
T3947	4839-4846	NNS	denotes	signals
T3946	4835-4838	DT	denotes	The
T3945	4833-4834	.	denotes	.
T3944	4827-4833	NN	denotes	buffer
T3943	4817-4826	VBG	denotes	stripping
T3942	4812-4816	IN	denotes	with
T3941	4805-4811	VBN	denotes	washed
T3940	4799-4804	VBG	denotes	being
T3939	4793-4798	IN	denotes	after
T3938	4791-4792	-RRB-	denotes	)
T3937	4786-4791	NNP	denotes	Maine
T3936	4784-4785	,	denotes	,
T3935	4780-4784	NNP	denotes	Saco
T3934	4778-4779	,	denotes	,
T3933	4769-4778	NNP	denotes	Biodesign
T3932	4767-4768	,	denotes	,
T3931	4754-4767	NN	denotes	dehydrogenase
T3930	4727-4753	JJ	denotes	glyceraldehyde-3-phosphate
T3929	4726-4727	-LRB-	denotes	(
T3928	4715-4725	JJ	denotes	anti-GAPDH
T3927	4710-4714	IN	denotes	with
T3926	4703-4709	VBN	denotes	probed
T3925	4698-4702	VBD	denotes	were
T3924	4688-4697	NNS	denotes	membranes
T3923	4683-4687	JJ	denotes	same
T3922	4679-4682	DT	denotes	The
T3921	4677-4678	.	denotes	.
T3920	4676-4677	-RRB-	denotes	)
T3919	4667-4676	NNP	denotes	Pharmacia
T3918	4658-4666	NNP	denotes	Amersham
T3917	4657-4658	-LRB-	denotes	(
T3916	4650-4656	NN	denotes	system
T3915	4646-4649	NNP	denotes	ECL
T3914	4642-4645	DT	denotes	the
T3913	4637-4641	IN	denotes	with
T3912	4628-4636	VBN	denotes	detected
T3911	4623-4627	VBD	denotes	were
T3910	4615-4622	NNS	denotes	signals
T3909	4611-4614	DT	denotes	The
T3908	4609-4610	.	denotes	.
T3907	4608-4609	-RRB-	denotes	)
T3906	4601-4608	NNP	denotes	PV-6001
T3905	4599-4600	,	denotes	,
T3904	4593-4599	NNP	denotes	Bridge
T3903	4586-4592	NNP	denotes	Golden
T3902	4585-4586	-LRB-	denotes	(
T3901	4574-4584	NNS	denotes	antibodies
T3900	4564-4573	JJ	denotes	secondary
T3899	4542-4563	JJ	denotes	peroxidase-conjugated
T3898	4537-4541	IN	denotes	with
T3897	4526-4536	NN	denotes	incubation
T3896	4523-4525	IN	denotes	by
T3895	4514-4522	VBN	denotes	followed
T3894	4512-4513	-RRB-	denotes	)
T3893	4506-4512	NNP	denotes	BA1717
T3892	4504-4505	,	denotes	,
T3891	4499-4504	JJ	denotes	WuHan
T3890	4498-4499	-LRB-	denotes	(
T3889	4489-4497	NN	denotes	antibody
T3888	4481-4488	JJ	denotes	primary
T3887	4469-4480	JJ	denotes	appropriate
T3886	4465-4468	DT	denotes	the
T3885	4460-4464	IN	denotes	with
T3884	4450-4459	VBN	denotes	incubated
T3883	4445-4449	VBD	denotes	were
T3882	4435-4444	NNS	denotes	membranes
T3881	4431-4434	NNP	denotes	ECL
T3880	4427-4430	DT	denotes	The
T3879	4425-4426	.	denotes	.
T3878	4424-4425	-RRB-	denotes	)
T3877	4422-4424	NNP	denotes	NJ
T3876	4420-4421	,	denotes	,
T3875	4410-4420	NNP	denotes	Piscataway
T3874	4408-4409	,	denotes	,
T3873	4399-4408	NNP	denotes	Pharmacia
T3872	4390-4398	NNP	denotes	Amersham
T3871	4389-4390	-LRB-	denotes	(
T3870	4379-4388	NNS	denotes	membranes
T3869	4375-4378	NNP	denotes	ECL
T3868	4368-4374	NNP	denotes	Hybond
T3867	4363-4367	IN	denotes	onto
T3866	4351-4362	VBN	denotes	transferred
T3865	4347-4350	CC	denotes	and
T3864	4343-4346	NN	denotes	gel
T3863	4324-4342	JJ	denotes	SDS-polyacrylamide
T3862	4319-4323	IN	denotes	with
T3861	4303-4318	VBN	denotes	electrophoresed
T3860	4301-4302	,	denotes	,
T3859	4290-4301	NNS	denotes	hemispheres
T3858	4281-4289	JJ	denotes	cerebral
T3857	4277-4280	NNP	denotes	ICH
T3856	4272-4276	IN	denotes	from
T3855	4263-4271	VBN	denotes	prepared
T3854	4258-4262	VBD	denotes	were
T3853	4249-4257	NNS	denotes	proteins
T3852	4240-4248	NNP	denotes	Cellular
T3851	4234-4239	NNP	denotes	Blots
T3850	4226-4233	JJ	denotes	Western
T3849	4224-4225	.	denotes	.
T3848	4221-4224	CD	denotes	2.5
T3662	4140-4141	,	denotes	,
T3661	4136-4140	NNP	denotes	Cruz
T3660	4130-4135	NNP	denotes	Santa
T3659	4129-4130	-LRB-	denotes	(
T3658	4124-4128	NNP	denotes	Inc.
T3657	4110-4123	NNP	denotes	Biotechnology
T3656	4105-4109	NNP	denotes	Cruz
T3655	4099-4104	NNP	denotes	Santa
T3654	4094-4098	IN	denotes	from
T3653	4085-4093	VBN	denotes	obtained
T3652	4080-4084	VBD	denotes	were
T3651	4078-4079	-RRB-	denotes	)
T3650	4071-4078	NN	denotes	SC-2018
T3649	4069-4070	,	denotes	,
T3648	4062-4069	NN	denotes	reagent
T3647	4058-4061	NNP	denotes	ABC
T3646	4057-4058	-LRB-	denotes	(
T3645	4047-4056	NNS	denotes	complexes
T3644	4013-4046	JJ	denotes	antibody-biotin-avidin-peroxidase
T3643	4009-4012	CC	denotes	and
T3642	4000-4008	NN	denotes	antibody
T3641	3990-3999	JJ	denotes	secondary
T3640	3977-3989	JJ	denotes	biotinylated
T3639	3973-3976	DT	denotes	The
T3638	3971-3972	.	denotes	.
T3637	3970-3971	-RRB-	denotes	)
T3636	3964-3970	NNP	denotes	BA1717
T3635	3962-3963	,	denotes	,
T3634	3957-3962	JJ	denotes	WuHan
T3633	3956-3957	-LRB-	denotes	(
T3632	3946-3955	NN	denotes	anti-TLR4
T3631	3939-3945	NN	denotes	rabbit
T3630	3935-3938	VBD	denotes	was
T3629	3926-3934	VBN	denotes	employed
T3628	3917-3925	NN	denotes	antibody
T3627	3909-3916	JJ	denotes	primary
T4183	4932-4947	NNP	denotes	Electrophoretic
T4182	4930-4931	.	denotes	.
T4181	4927-4930	CD	denotes	2.6
T3626	3905-3908	DT	denotes	The
T3625	3903-3904	.	denotes	.
T3624	3902-3903	NN	denotes	]
T3623	3901-3902	CD	denotes	8
T3622	3900-3901	JJ	denotes	[
T3621	3889-3899	RB	denotes	previously
T3620	3879-3888	VBN	denotes	described
T3619	3876-3878	IN	denotes	as
T3618	3866-3875	VBN	denotes	performed
T3617	3862-3865	VBD	denotes	was
T3616	3857-3861	CD	denotes	TLR4
T3615	3853-3856	IN	denotes	for
T3614	3844-3852	VBG	denotes	staining
T3613	3840-3843	NNP	denotes	IHC
T3612	3836-3839	DT	denotes	The
T3611	3834-3835	-RRB-	denotes	)
T3610	3831-3834	NNP	denotes	IHC
T3609	3830-3831	-LRB-	denotes	(
T3608	3809-3829	NNP	denotes	Immunohistochemistry
T3607	3807-3808	.	denotes	.
T3606	3804-3807	CD	denotes	2.4
T3373	3801-3802	.	denotes	.
T3372	3797-3801	NNP	denotes	mRNA
T3371	3789-3796	VB	denotes	β-actin
T3370	3786-3788	TO	denotes	to
T3369	3781-3785	NNP	denotes	mRNA
T3368	3776-3780	CD	denotes	TLR4
T3367	3773-3775	IN	denotes	of
T3366	3767-3772	NN	denotes	ratio
T3365	3763-3766	DT	denotes	the
T3364	3760-3762	IN	denotes	as
T3363	3750-3759	VBN	denotes	expressed
T3362	3746-3749	VBD	denotes	was
T3361	3741-3745	NNP	denotes	mRNA
T3360	3736-3740	CD	denotes	TLR4
T3359	3733-3735	IN	denotes	of
T3358	3723-3732	NN	denotes	abundance
T3357	3719-3722	DT	denotes	The
T3356	3717-3718	.	denotes	.
T3355	3708-3717	NNS	denotes	densities
T3354	3703-3707	NN	denotes	band
T3353	3693-3702	VB	denotes	determine
T3352	3690-3692	TO	denotes	to
T3351	3685-3689	VBN	denotes	used
T3350	3680-3684	VBD	denotes	were
T3349	3671-3679	NN	denotes	software
T3348	3662-3670	NNP	denotes	Analysis
T3347	3658-3661	CC	denotes	and
T3346	3646-3657	NNP	denotes	Acquisition
T3345	3640-3645	NN	denotes	Image
T3344	3638-3639	.	denotes	.
T3343	3635-3638	NN	denotes	gel
T3342	3627-3634	JJ	denotes	agarose
T3341	3619-3626	IN	denotes	through
T3340	3603-3618	VBN	denotes	electrophoresed
T3339	3598-3602	VBD	denotes	were
T3338	3589-3597	NNS	denotes	products
T3337	3585-3588	NNP	denotes	PCR
T3336	3583-3584	.	denotes	.
T3335	3577-3583	NNS	denotes	cycles
T3334	3574-3576	CD	denotes	31
T3333	3568-3573	RB	denotes	after
T3332	3560-3567	VBN	denotes	stopped
T3331	3556-3559	VBD	denotes	was
T3330	3547-3555	NN	denotes	reaction
T3329	3543-3546	DT	denotes	The
T3328	3541-3542	.	denotes	.
T3327	3540-3541	PRP	denotes	s
T3326	3537-3539	CD	denotes	60
T3325	3535-3536	NNP	denotes	C
T3324	3534-3535	NN	denotes	°
T3323	3532-3534	CD	denotes	72
T3322	3528-3531	CC	denotes	and
T3321	3526-3527	,	denotes	,
T3320	3525-3526	PRP	denotes	s
T3319	3522-3524	CD	denotes	40
T3318	3520-3521	NNP	denotes	C
T3317	3519-3520	NN	denotes	°
T3316	3517-3519	CD	denotes	55
T3315	3515-3516	,	denotes	,
T3314	3514-3515	PRP	denotes	s
T3313	3511-3513	CD	denotes	40
T3312	3509-3510	NNP	denotes	C
T3311	3508-3509	NN	denotes	°
T3310	3506-3508	CD	denotes	94
T3309	3504-3505	,	denotes	,
T3308	3501-3504	NN	denotes	min
T3307	3499-3500	CD	denotes	2
T3306	3497-3498	NNP	denotes	C
T3305	3496-3497	NN	denotes	°
T3304	3494-3496	CD	denotes	94
T3303	3492-3493	:	denotes	:
T3302	3483-3492	NN	denotes	condition
T3301	3473-3482	JJ	denotes	following
T3300	3469-3472	DT	denotes	the
T3299	3466-3468	IN	denotes	at
T3298	3456-3465	VBN	denotes	performed
T3297	3451-3455	VBD	denotes	were
T3296	3444-3450	NNS	denotes	cycles
T3295	3435-3443	NN	denotes	Reaction
T3294	3433-3434	.	denotes	.
T3293	3432-3433	NN	denotes	′
T3292	3411-3432	NN	denotes	CCAGGATAGAGCCACCAAT-3
T3291	3410-3411	:	denotes	-
T3290	3409-3410	SYM	denotes	′
T3289	3408-3409	CD	denotes	5
T3288	3406-3407	:	denotes	:
T3287	3399-3406	NN	denotes	reverse
T3286	3397-3398	:	denotes	;
T3285	3396-3397	NN	denotes	′
T3284	3375-3396	NN	denotes	GCCAACCGTGAAAAAGATG-3
T3283	3374-3375	:	denotes	-
T3282	3373-3374	SYM	denotes	′
T3281	3372-3373	CD	denotes	5
T3280	3370-3371	:	denotes	:
T3279	3363-3370	RB	denotes	forward
T3278	3361-3362	:	denotes	:
T3277	3360-3361	-RRB-	denotes	)
T3276	3358-3360	NN	denotes	bp
T3275	3354-3357	CD	denotes	701
T3274	3353-3354	-LRB-	denotes	(
T3273	3345-3352	NN	denotes	β-actin
T3272	3343-3344	:	denotes	;
T3271	3342-3343	NN	denotes	′
T3270	3320-3342	NN	denotes	CAGGATGACACCATTGAAGC-3
T3269	3319-3320	:	denotes	-
T3268	3318-3319	SYM	denotes	′
T3267	3317-3318	CD	denotes	5
T3266	3315-3316	:	denotes	:
T3265	3308-3315	NN	denotes	reverse
T3264	3306-3307	:	denotes	;
T3263	3305-3306	NN	denotes	′
T3262	3283-3305	NN	denotes	AGCTTTGGTCAGTTGGCTCT-3
T3261	3282-3283	:	denotes	-
T3260	3281-3282	SYM	denotes	′
T3259	3280-3281	CD	denotes	5
T3258	3278-3279	:	denotes	:
T3257	3271-3278	RB	denotes	forward
T3256	3269-3270	:	denotes	:
T3255	3268-3269	-RRB-	denotes	)
T3254	3266-3268	NN	denotes	bp
T3253	3262-3265	CD	denotes	145
T3252	3261-3262	-LRB-	denotes	(
T3251	3256-3260	CD	denotes	TLR4
T3250	3254-3255	.	denotes	.
T3249	3247-3254	NNP	denotes	GenBank
T3248	3244-3246	IN	denotes	in
T3247	3235-3243	VBD	denotes	reported
T3246	3225-3234	NNS	denotes	sequences
T3245	3220-3224	NN	denotes	gene
T3244	3212-3219	JJ	denotes	β-actin
T3243	3208-3211	CC	denotes	and
T3242	3203-3207	CD	denotes	TLR4
T3241	3199-3202	DT	denotes	the
T3240	3196-3198	TO	denotes	to
T3239	3186-3195	VBG	denotes	according
T3238	3177-3185	VBN	denotes	designed
T3237	3172-3176	VBD	denotes	were
T3236	3162-3171	NNS	denotes	sequences
T3235	3155-3161	NN	denotes	primer
T3234	3151-3154	NNP	denotes	PCR
T3233	3147-3150	DT	denotes	The
T3232	3145-3146	.	denotes	.
T3231	3138-3145	NN	denotes	control
T3230	3129-3137	JJ	denotes	internal
T3229	3126-3128	DT	denotes	an
T3228	3123-3125	IN	denotes	as
T3227	3118-3122	VBN	denotes	used
T3226	3114-3117	VBD	denotes	was
T3225	3112-3113	-RRB-	denotes	)
T3224	3105-3112	NN	denotes	β-actin
T3223	3104-3105	-LRB-	denotes	(
T3222	3099-3103	NN	denotes	gene
T3221	3094-3098	VB	denotes	keep
T3220	3088-3093	NN	denotes	house
T3219	3084-3087	DT	denotes	The
T3218	3082-3083	.	denotes	.
T3217	3072-3082	NN	denotes	polymerase
T3216	3068-3071	NNP	denotes	DNA
T3215	3064-3067	NNP	denotes	Taq
T3214	3061-3063	CD	denotes	2u
T3213	3057-3060	CC	denotes	and
T3212	3055-3056	,	denotes	,
T3211	3048-3055	NNS	denotes	primers
T3210	3045-3047	IN	denotes	of
T3209	3042-3044	NN	denotes	μM
T3208	3038-3041	CD	denotes	200
T3207	3036-3037	,	denotes	,
T3206	3032-3036	NNP	denotes	dNTP
T3205	3027-3031	DT	denotes	each
T3204	3024-3026	IN	denotes	of
T3203	3021-3023	NN	denotes	μM
T3202	3017-3020	CD	denotes	200
T3201	3015-3016	,	denotes	,
T3200	3010-3015	NNP	denotes	MgCl2
T3199	3007-3009	NNP	denotes	mM
T3198	3003-3006	CD	denotes	1.5
T3087	2379-2383	IN	denotes	from
T3086	2370-2378	VBN	denotes	isolated
T3085	2365-2369	VBD	denotes	were
T3084	2357-2364	NNS	denotes	samples
T3083	2353-2356	NNP	denotes	RNA
T3082	2347-2352	JJ	denotes	total
T3081	2345-2346	,	denotes	,
T3080	2338-2345	NNP	denotes	Briefly
T3079	2331-2337	NNP	denotes	RT-PCR
T3078	2328-2330	IN	denotes	of
T3077	2321-2327	NNP	denotes	Method
T3076	2317-2320	DT	denotes	the
T3075	2314-2316	IN	denotes	by
T3074	2309-2313	NNP	denotes	mRNA
T3073	2304-2308	NNP	denotes	TLR4
T3072	2301-2303	IN	denotes	of
T3071	2291-2300	NNP	denotes	Detection
T3070	2289-2290	.	denotes	.
T3069	2286-2289	CD	denotes	2.3
T2535	1634-1641	JJ	denotes	sterile
T2534	1632-1633	DT	denotes	a
T2533	1626-1631	VBG	denotes	Using
T2532	1624-1625	.	denotes	.
T2531	1617-1624	NN	denotes	midline
T2530	1613-1616	DT	denotes	the
T2529	1607-1612	IN	denotes	along
T2528	1599-1606	VBN	denotes	incised
T2527	1595-1598	VBD	denotes	was
T2526	1589-1594	NN	denotes	scalp
T2525	1585-1588	DT	denotes	the
T2524	1581-1584	CC	denotes	and
T2523	1579-1580	,	denotes	,
T2522	1574-1579	NN	denotes	frame
T2521	1561-1573	JJ	denotes	stereotactic
T2520	1559-1560	DT	denotes	a
T2519	1556-1558	IN	denotes	in
T2518	1545-1555	VBN	denotes	positioned
T2517	1540-1544	VBD	denotes	were
T2516	1535-1539	NNS	denotes	rats
T2515	1531-1534	DT	denotes	The
T2514	1529-1530	.	denotes	.
T2513	1523-1529	NN	denotes	system
T2512	1515-1522	NN	denotes	heating
T2511	1495-1514	JJ	denotes	feedback-controlled
T2510	1493-1494	DT	denotes	a
T2509	1487-1492	VBG	denotes	using
T2508	1479-1486	NN	denotes	surgery
T2507	1475-1478	DT	denotes	the
T2506	1468-1474	IN	denotes	during
T2505	1466-1467	NNP	denotes	C
T2504	1465-1466	CD	denotes	°
T2503	1463-1465	CD	denotes	37
T2502	1460-1462	IN	denotes	at
T2501	1449-1459	VBN	denotes	maintained
T2500	1445-1448	VBD	denotes	was
T2499	1433-1444	NN	denotes	temperature
T2498	1431-1432	-RRB-	denotes	)
T2497	1425-1431	JJ	denotes	rectal
T2496	1424-1425	-LRB-	denotes	(
T2495	1419-1423	NN	denotes	body
T2494	1415-1418	DT	denotes	The
T2493	1413-1414	.	denotes	.
T2492	1412-1413	-RRB-	denotes	)
T2491	1407-1412	NN	denotes	mg/kg
T2490	1404-1406	CD	denotes	60
T2489	1403-1404	-LRB-	denotes	(
T2488	1389-1402	JJ	denotes	pentobarbital
T2487	1382-1388	NN	denotes	sodium
T2486	1365-1381	JJ	denotes	intra-peritoneal
T2485	1360-1364	IN	denotes	with
T2484	1347-1359	JJ	denotes	anesthetized
T2483	1342-1346	VBD	denotes	were
T2482	1337-1341	NNS	denotes	Rats
T2481	1335-1336	.	denotes	.
T2480	1328-1335	NNS	denotes	ganglia
T2479	1322-1327	JJ	denotes	basal
T2478	1317-1321	JJ	denotes	left
T2477	1314-1316	IN	denotes	in
T2476	1306-1313	VBN	denotes	induced
T2475	1302-1305	VBD	denotes	was
T2474	1298-1301	NNP	denotes	ICH
T2473	1295-1297	IN	denotes	of
T2472	1289-1294	NN	denotes	model
T2471	1285-1288	NN	denotes	rat
T2470	1283-1284	,	denotes	,
T2469	1278-1283	NN	denotes	study
T2468	1270-1277	JJ	denotes	present
T2467	1266-1269	DT	denotes	the
T2466	1263-1265	IN	denotes	in
T2465	1261-1262	,	denotes	,
T2464	1251-1261	RB	denotes	clinically
T2463	1243-1250	NN	denotes	ganglia
T2462	1237-1242	JJ	denotes	basal
T2461	1233-1236	DT	denotes	the
T2460	1230-1232	IN	denotes	in
T2459	1224-1229	VBP	denotes	occur
T2458	1212-1223	NNS	denotes	hemorrhages
T2457	1198-1211	JJ	denotes	intracerebral
T2456	1193-1197	JJS	denotes	most
T2455	1187-1192	IN	denotes	Since
T2454	1176-1186	NNP	denotes	Hemorrhage
T2453	1162-1175	NNP	denotes	Intracerebral
T2452	1159-1161	IN	denotes	of
T2451	1149-1158	NNP	denotes	Induction
T2450	1147-1148	.	denotes	.
T2449	1144-1147	CD	denotes	2.2
T3197	3001-3002	,	denotes	,
T3196	2995-3001	NN	denotes	buffer
T3195	2991-2994	NNP	denotes	PCR
T3194	2989-2990	CD	denotes	×
T3193	2988-2989	CD	denotes	1
T3192	2983-2987	IN	denotes	with
T3191	2974-2982	NN	denotes	fragment
T3190	2969-2973	NN	denotes	gene
T3189	2964-2968	CD	denotes	TLR4
T3188	2960-2963	DT	denotes	the
T3187	2952-2959	VB	denotes	amplify
T3186	2949-2951	TO	denotes	to
T3185	2944-2948	VBN	denotes	used
T3184	2940-2943	VBD	denotes	was
T3183	2935-2939	NNP	denotes	cDNA
T3182	2932-2934	IN	denotes	of
T3181	2921-2931	NN	denotes	microliter
T3180	2917-2920	CD	denotes	One
T3179	2915-2916	.	denotes	.
T3178	2907-2915	NN	denotes	supplier
T3177	2903-2906	DT	denotes	the
T3176	2900-2902	IN	denotes	by
T3175	2890-2899	VBN	denotes	described
T3174	2879-2889	NNS	denotes	conditions
T3173	2873-2878	IN	denotes	under
T3172	2871-2872	-RRB-	denotes	)
T3171	2868-2871	NNP	denotes	Inc
T3170	2860-2867	JJ	denotes	promega
T3169	2859-2860	-LRB-	denotes	(
T3168	2852-2858	NNP	denotes	RT-PCR
T3167	2848-2851	IN	denotes	for
T3166	2844-2847	NNP	denotes	Kit
T3165	2834-2843	NNP	denotes	Synthesis
T3164	2821-2833	NNP	denotes	First-Strand
T3163	2809-2820	NNP	denotes	Superscript
T3162	2803-2808	VBG	denotes	using
T3161	2796-2802	NN	denotes	system
T3160	2787-2795	NN	denotes	reaction
T3159	2784-2786	NN	denotes	μL
T3158	2781-2783	CD	denotes	20
T3157	2778-2780	IN	denotes	in
T3156	2773-2777	NNP	denotes	cDNA
T3155	2768-2772	IN	denotes	into
T3154	2748-2767	VBN	denotes	reverse-transcribed
T3153	2744-2747	VBD	denotes	was
T3152	2740-2743	NNP	denotes	RNA
T3151	2738-2739	.	denotes	.
T3150	2734-2738	NNP	denotes	g/mL
T3149	2730-2733	CD	denotes	1.0
T3148	2727-2729	TO	denotes	to
T3147	2718-2726	VBN	denotes	adjusted
T3146	2713-2717	VBD	denotes	were
T3145	2708-2712	NNP	denotes	mRNA
T3144	2705-2707	IN	denotes	of
T3143	2690-2704	NNS	denotes	concentrations
T3142	2686-2689	DT	denotes	the
T3141	2684-2685	,	denotes	,
T3140	2680-2684	RB	denotes	Then
T3139	2678-2679	.	denotes	.
T3138	2668-2678	NN	denotes	wavelength
T3137	2665-2667	NN	denotes	nm
T3136	2660-2664	CD	denotes	A280
T3135	2656-2659	CC	denotes	and
T3134	2651-2655	CD	denotes	A260
T3133	2648-2650	IN	denotes	at
T3132	2640-2647	NN	denotes	density
T3131	2632-2639	JJ	denotes	optical
T3130	2622-2631	VBG	denotes	measuring
T3129	2619-2621	IN	denotes	by
T3128	2617-2618	-RRB-	denotes	)
T3127	2600-2617	NNP	denotes	Spectrophotometer
T3126	2592-2599	NNP	denotes	ND-1000
T3125	2591-2592	-LRB-	denotes	(
T3124	2582-2590	NNP	denotes	Nanodrop
T3123	2580-2581	DT	denotes	a
T3122	2577-2579	IN	denotes	on
T3121	2566-2576	VBN	denotes	determined
T3120	2562-2565	VBD	denotes	was
T3119	2558-2561	NNP	denotes	RNA
T3118	2552-2557	JJ	denotes	total
T3117	2549-2551	IN	denotes	of
T3116	2540-2548	NN	denotes	quantity
T3115	2536-2539	DT	denotes	The
T3114	2534-2535	.	denotes	.
T3113	2522-2534	NNS	denotes	instructions
T3112	2519-2521	POS	denotes	's
T3111	2507-2519	NN	denotes	manufacturer
T3110	2503-2506	DT	denotes	the
T3109	2500-2502	TO	denotes	to
T3108	2490-2499	VBG	denotes	according
T3107	2488-2489	-RRB-	denotes	)
T3106	2483-2488	NNP	denotes	China
T3105	2481-2482	,	denotes	,
T3104	2479-2481	NNP	denotes	HZ
T3103	2477-2478	,	denotes	,
T3102	2470-2477	NNP	denotes	BioFlux
T3101	2469-2470	-LRB-	denotes	(
T3100	2465-2468	NN	denotes	Kit
T3099	2455-2464	NN	denotes	isolation
T3098	2451-2454	NNP	denotes	RNA
T3097	2445-2450	NNP	denotes	Total
T3096	2443-2444	NNP	denotes	P
T3095	2436-2442	RB	denotes	Simply
T3094	2430-2435	VBG	denotes	using
T3093	2422-2429	NN	denotes	ganglia
T3092	2416-2421	JJ	denotes	basal
T3091	2411-2415	JJ	denotes	left
T3090	2408-2410	IN	denotes	in
T3089	2400-2407	NNS	denotes	regions
T2216	1141-1142	.	denotes	.
T2215	1136-1141	NNP	denotes	Blots
T2214	1128-1135	NNP	denotes	Western
T2213	1124-1127	CC	denotes	and
T2212	1117-1123	NNP	denotes	RT-PCR
T2211	1113-1116	IN	denotes	for
T2210	1111-1112	NNP	denotes	C
T2209	1110-1111	CD	denotes	°
T2208	1108-1110	CD	denotes	80
T2207	1102-1107	CC	denotes	minus
T2206	1099-1101	IN	denotes	at
T2205	1092-1098	VBN	denotes	stored
T2204	1088-1091	CC	denotes	and
T2203	1080-1087	VBN	denotes	removed
T2202	1075-1079	VBD	denotes	were
T2201	1068-1074	NNS	denotes	brains
T2200	1064-1067	DT	denotes	The
T2199	1062-1063	.	denotes	.
T2198	1054-1062	NNP	denotes	Ketamine
T2197	1051-1053	IN	denotes	of
T2196	1041-1050	NN	denotes	injection
T2195	1036-1040	NN	denotes	dose
T2194	1031-1035	IN	denotes	over
T2193	1028-1030	IN	denotes	by
T2192	1017-1027	VBN	denotes	sacrificed
T2191	1012-1016	VBD	denotes	were
T2190	1006-1011	NN	denotes	point
T2189	1001-1005	NN	denotes	time
T2188	996-1000	DT	denotes	each
T2187	993-995	IN	denotes	at
T2186	987-992	NN	denotes	group
T2185	982-986	DT	denotes	each
T2184	979-981	IN	denotes	in
T2183	974-978	NNS	denotes	rats
T2182	969-973	CD	denotes	Five
T2181	967-968	.	denotes	.
T2180	966-967	NN	denotes	]
T2179	965-966	CD	denotes	8
T2178	964-965	JJ	denotes	[
T2177	953-963	RB	denotes	previously
T2176	943-952	VBN	denotes	described
T2175	940-942	IN	denotes	as
T2174	938-939	-RRB-	denotes	)
T2173	936-938	NN	denotes	μm
T2172	934-935	CD	denotes	7
T2171	933-934	-LRB-	denotes	(
T2170	924-932	NNS	denotes	sections
T2169	919-923	IN	denotes	into
T2168	915-918	VBD	denotes	cut
T2167	911-914	CC	denotes	and
T2166	909-910	,	denotes	,
T2165	901-909	NN	denotes	paraffin
T2164	898-900	IN	denotes	in
T2163	889-897	VBN	denotes	embedded
T2162	887-888	,	denotes	,
T2161	878-887	VBN	denotes	postfixed
T2160	876-877	,	denotes	,
T2159	869-876	VBN	denotes	removed
T2158	864-868	VBD	denotes	were
T2157	857-863	NNS	denotes	brains
T2156	853-856	DT	denotes	The
T2155	851-852	.	denotes	.
T2154	850-851	-RRB-	denotes	)
T2153	847-850	CD	denotes	7.4
T2152	845-846	SYM	denotes	=
T2151	842-844	NNP	denotes	pH
T2150	841-842	-LRB-	denotes	(
T2149	828-840	NN	denotes	formaldehyde
T2148	823-827	NNP	denotes	Para
T2147	814-822	VBD	denotes	buffered
T2146	812-813	NN	denotes	%
T2145	811-812	CD	denotes	4
T2144	808-810	IN	denotes	by
T2143	799-807	VBN	denotes	followed
T2142	792-798	NN	denotes	saline
T2141	785-791	JJ	denotes	normal
T2140	782-784	IN	denotes	of
T2139	772-781	NN	denotes	perfusion
T2138	757-771	RB	denotes	transcardially
T2137	754-756	IN	denotes	by
T2136	743-753	VBN	denotes	sacrificed
T2135	738-742	VBD	denotes	were
T2134	732-737	NN	denotes	point
T2665	2275-2283	VBN	denotes	injected
T2664	2271-2274	VBD	denotes	was
T2663	2264-2270	NN	denotes	saline
T2662	2257-2263	JJ	denotes	normal
T2661	2254-2256	NN	denotes	μL
T2660	2251-2253	CD	denotes	75
T2659	2246-2250	RB	denotes	only
T2658	2244-2245	,	denotes	,
T2657	2240-2244	NNS	denotes	rats
T2656	2232-2239	NN	denotes	control
T2655	2225-2231	NN	denotes	saline
T2654	2218-2224	JJ	denotes	normal
T2653	2214-2217	DT	denotes	the
T2652	2210-2213	IN	denotes	For
T2651	2208-2209	.	denotes	.
T2650	2201-2208	NNS	denotes	sutures
T2649	2196-2200	IN	denotes	with
T2648	2189-2195	VBN	denotes	closed
T2647	2185-2188	VBD	denotes	was
T2646	2176-2184	NN	denotes	incision
T2645	2171-2175	NN	denotes	skin
T2644	2167-2170	DT	denotes	the
T2643	2163-2166	CC	denotes	and
T2642	2161-2162	,	denotes	,
T2641	2157-2161	NN	denotes	hole
T2640	2152-2156	NN	denotes	burr
T2639	2148-2151	DT	denotes	the
T2638	2141-2147	IN	denotes	around
T2637	2134-2140	VBN	denotes	placed
T2636	2130-2133	VBD	denotes	was
T2635	2125-2129	NN	denotes	glue
T2634	2111-2124	VBP	denotes	cyanoacrylate
T2633	2109-2110	,	denotes	,
T2632	2102-2109	RB	denotes	quickly
T2631	2092-2101	VBN	denotes	withdrawn
T2630	2088-2091	VBD	denotes	was
T2629	2081-2087	NN	denotes	needle
T2628	2077-2080	DT	denotes	the
T2627	2075-2076	,	denotes	,
T2626	2067-2075	NN	denotes	infusion
T2625	2063-2066	DT	denotes	the
T2624	2060-2062	IN	denotes	of
T2623	2049-2059	NN	denotes	completion
T2622	2045-2048	DT	denotes	the
T2621	2039-2044	IN	denotes	After
T2620	2037-2038	.	denotes	.
T2619	2033-2037	NN	denotes	pump
T2618	2019-2032	NN	denotes	microinfusion
T2617	2017-2018	DT	denotes	a
T2616	2014-2016	IN	denotes	of
T2615	2010-2013	NN	denotes	use
T2614	2006-2009	DT	denotes	the
T2613	2001-2005	IN	denotes	with
T2612	1997-2000	NN	denotes	min
T2611	1996-1997	NNP	denotes	/
T2610	1994-1996	NNP	denotes	μL
T2609	1991-1993	CD	denotes	20
T2608	1988-1990	IN	denotes	of
T2607	1983-1987	NN	denotes	rate
T2606	1981-1982	DT	denotes	a
T2605	1978-1980	IN	denotes	at
T2604	1970-1977	VBN	denotes	infused
T2603	1966-1969	VBD	denotes	was
T2602	1960-1965	NN	denotes	blood
T2601	1954-1959	JJ	denotes	whole
T2600	1943-1953	JJ	denotes	autologous
T2599	1940-1942	IN	denotes	of
T2598	1937-1939	NN	denotes	μL
T2597	1934-1936	CD	denotes	75
T2596	1932-1933	DT	denotes	a
T2595	1930-1931	,	denotes	,
T2594	1926-1930	RB	denotes	Then
T2593	1924-1925	.	denotes	.
T2592	1923-1924	-RRB-	denotes	)
T2591	1916-1923	NN	denotes	midline
T2590	1912-1915	DT	denotes	the
T2589	1909-1911	TO	denotes	to
T2588	1901-1908	NN	denotes	lateral
T2587	1898-1900	NN	denotes	mm
T2586	1894-1897	CD	denotes	3.0
T2585	1890-1893	CC	denotes	and
T2584	1888-1889	,	denotes	,
T2583	1881-1888	NN	denotes	ventral
T2582	1878-1880	NN	denotes	mm
T2581	1874-1877	CD	denotes	6.0
T2580	1872-1873	,	denotes	,
T2579	1864-1872	NN	denotes	anterior
T2578	1861-1863	NN	denotes	mm
T2577	1857-1860	CD	denotes	0.2
T2576	1855-1856	:	denotes	:
T2575	1844-1855	VBZ	denotes	coordinates
T2574	1843-1844	-LRB-	denotes	(
T2573	1834-1842	NN	denotes	guidance
T2572	1821-1833	JJ	denotes	stereotactic
T2571	1815-1820	IN	denotes	under
T2570	1807-1814	NN	denotes	ganglia
T2569	1801-1806	JJ	denotes	basal
T2568	1796-1800	JJ	denotes	left
T2567	1792-1795	DT	denotes	the
T2566	1787-1791	IN	denotes	into
T2565	1778-1786	VBN	denotes	inserted
T2564	1774-1777	VBD	denotes	was
T2563	1767-1773	NN	denotes	needle
T2562	1758-1766	JJ	denotes	26-gauge
T2561	1752-1757	JJ	denotes	blunt
T2560	1750-1751	DT	denotes	A
T2559	1748-1749	.	denotes	.
T2558	1741-1748	NN	denotes	midline
T2557	1737-1740	DT	denotes	the
T2556	1734-1736	TO	denotes	to
T2555	1726-1733	NN	denotes	lateral
T2554	1723-1725	NN	denotes	mm
T2553	1721-1722	CD	denotes	3
T2552	1714-1720	NN	denotes	suture
T2551	1706-1713	JJ	denotes	coronal
T2550	1701-1705	VBN	denotes	left
T2549	1697-1700	DT	denotes	the
T2548	1694-1696	IN	denotes	on
T2547	1688-1693	NN	denotes	skull
T2546	1684-1687	DT	denotes	the
T2545	1681-1683	IN	denotes	in
T2544	1674-1680	VBN	denotes	opened
T2543	1670-1673	VBD	denotes	was
T2542	1665-1669	NN	denotes	hole
T2541	1660-1664	NN	denotes	burr
T2540	1657-1659	NN	denotes	mm
T2539	1655-1656	CD	denotes	1
T2538	1653-1654	DT	denotes	a
T2537	1651-1652	,	denotes	,
T2536	1642-1651	NN	denotes	technique
T2133	727-731	NN	denotes	time
T2132	722-726	DT	denotes	each
T2131	719-721	IN	denotes	at
T2130	713-718	NN	denotes	group
T2129	708-712	DT	denotes	each
T2128	705-707	IN	denotes	in
T2127	700-704	NNS	denotes	rats
T2126	695-699	CD	denotes	Five
T2125	693-694	.	denotes	.
T2124	681-693	RB	denotes	respectively
T2123	679-680	,	denotes	,
T2122	670-679	NN	denotes	injection
T2121	666-669	DT	denotes	the
T2120	660-665	IN	denotes	after
T2119	655-659	NNS	denotes	days
T2118	653-654	CD	denotes	7
T2117	649-652	CC	denotes	and
T2116	644-648	NNS	denotes	days
T2115	642-643	CD	denotes	3
T2114	640-641	,	denotes	,
T2113	637-640	NN	denotes	day
T2112	635-636	CD	denotes	1
T2111	633-634	,	denotes	,
T2110	628-633	NNS	denotes	hours
T2109	626-627	CD	denotes	6
T2108	623-625	IN	denotes	on
T2107	612-622	VBN	denotes	sacrificed
T2106	607-611	VBD	denotes	were
T2105	603-606	CC	denotes	and
T2104	597-602	NN	denotes	blood
T2103	591-596	JJ	denotes	whole
T2102	580-590	JJ	denotes	autologous
T2101	577-579	CC	denotes	or
T2100	570-576	NN	denotes	saline
T2099	563-569	JJ	denotes	normal
T2098	560-562	IN	denotes	of
T2097	550-559	NN	denotes	injection
T2096	535-549	JJ	denotes	intra-cerebral
T2095	532-534	TO	denotes	to
T2094	522-531	VBN	denotes	subjected
T2093	517-521	VBD	denotes	were
T2092	512-516	NNS	denotes	rats
T2091	508-511	DT	denotes	The
T2090	506-507	.	denotes	.
T2089	496-506	NNP	denotes	University
T2088	488-495	NNP	denotes	Medical
T2087	482-487	NNP	denotes	China
T2086	480-481	,	denotes	,
T2085	471-480	NNP	denotes	Committee
T2084	467-470	NNP	denotes	Use
T2083	463-466	CC	denotes	and
T2082	458-462	NNP	denotes	Care
T2010	97-109	JJ	denotes	Experimental
T2009	94-96	IN	denotes	by
T2008	85-93	VBN	denotes	provided
T2007	80-84	VBD	denotes	were
T2006	78-79	-RRB-	denotes	)
T2005	77-78	NN	denotes	g
T2004	73-76	CD	denotes	300
T2003	70-72	TO	denotes	to
T2002	68-69	NN	denotes	g
T2001	64-67	CD	denotes	250
T2000	62-63	:	denotes	:
T1999	56-62	NN	denotes	weight
T1998	51-55	NN	denotes	body
T1997	49-50	,	denotes	,
T1996	45-49	NN	denotes	male
T1995	44-45	-LRB-	denotes	(
T1994	39-43	NNS	denotes	rats
T1993	32-38	NNP	denotes	Wister
T1992	25-31	CD	denotes	Ninety
T1991	17-24	NNS	denotes	Animals
T1990	15-16	.	denotes	.
T1989	12-15	CD	denotes	2.1
T2081	451-457	NNP	denotes	Animal
T2080	447-450	DT	denotes	the
T2079	444-446	IN	denotes	by
T2078	435-443	VBN	denotes	approved
T2077	431-434	VBD	denotes	was
T2076	423-430	NNS	denotes	animals
T2075	419-422	IN	denotes	for
T2074	414-418	VBN	denotes	used
T2073	405-413	NN	denotes	protocol
T2072	392-404	JJ	denotes	experimental
T2071	388-391	DT	denotes	The
T2070	386-387	.	denotes	.
T2069	385-386	-RRB-	denotes	)
T2068	383-385	CD	denotes	40
T2067	381-382	SYM	denotes	=
T2066	379-380	JJ	denotes	n
T2065	377-378	,	denotes	,
T2064	374-377	NNP	denotes	ICH
T2063	373-374	-LRB-	denotes	(
T2062	367-372	NN	denotes	group
T2061	356-366	NN	denotes	hemorrhage
T2060	341-355	JJ	denotes	intra-cerebral
T2059	337-340	CC	denotes	and
T2058	335-336	-RRB-	denotes	)
T2057	328-335	NN	denotes	control
T2056	325-327	IN	denotes	as
T2055	318-324	NN	denotes	saline
T2054	311-317	JJ	denotes	normal
T2053	308-310	IN	denotes	of
T2052	298-307	NN	denotes	injection
T2051	283-297	JJ	denotes	intra-cerebral
T2050	281-282	,	denotes	,
T2049	279-281	CD	denotes	40
T2048	277-278	SYM	denotes	=
T2047	275-276	JJ	denotes	n
T2046	273-274	,	denotes	,
T2045	272-273	NNP	denotes	S
T2044	271-272	-LRB-	denotes	(
T2043	263-270	NN	denotes	control
T2042	256-262	NN	denotes	saline
T2041	249-255	JJ	denotes	normal
T2040	247-248	,	denotes	,
T2039	246-247	-RRB-	denotes	)
T2038	237-246	NN	denotes	treatment
T2037	233-236	DT	denotes	any
T2036	225-232	IN	denotes	without
T2035	223-224	,	denotes	,
T2034	221-223	CD	denotes	10
T2033	219-220	SYM	denotes	=
T2032	217-218	JJ	denotes	n
T2031	215-216	,	denotes	,
T2030	214-215	NNP	denotes	N
T2029	213-214	-LRB-	denotes	(
T2028	207-212	NN	denotes	group
T2027	200-206	JJ	denotes	normal
T2026	198-199	:	denotes	:
T2025	192-198	NNS	denotes	groups
T2024	186-191	CD	denotes	three
T2023	183-185	TO	denotes	to
T2022	174-182	RB	denotes	randomly
T2021	165-173	VBN	denotes	assigned
T2020	160-164	VBD	denotes	were
T2019	155-159	NNS	denotes	rats
T2018	151-154	DT	denotes	The
T2017	149-150	.	denotes	.
T2016	139-149	NNP	denotes	University
T2015	131-138	NNP	denotes	Medical
T2014	125-130	NNP	denotes	China
T2013	123-124	,	denotes	,
T2012	117-123	NNP	denotes	Center
T2011	110-116	NNP	denotes	Animal
T3675	4218-4219	.	denotes	.
T3674	4210-4218	NNS	denotes	controls
T3673	4201-4209	JJ	denotes	negative
T3672	4198-4200	IN	denotes	as
T3671	4191-4197	VBD	denotes	served
T3670	4180-4190	NNS	denotes	antibodies
T3669	4172-4179	JJ	denotes	primary
T3668	4164-4171	IN	denotes	without
T3667	4154-4163	VBN	denotes	processed
T3666	4147-4153	NNS	denotes	Slides
T3665	4145-4146	.	denotes	.
T3664	4144-4145	-RRB-	denotes	)
T3663	4142-4144	NNP	denotes	CA
T4239	5253-5254	.	denotes	.
T4238	5246-5253	NNS	denotes	minutes
T4237	5243-5245	CD	denotes	40
T4236	5240-5242	IN	denotes	of
T4235	5234-5239	NN	denotes	total
T4234	5232-5233	DT	denotes	a
T4233	5228-5231	IN	denotes	for
T4232	5226-5227	,	denotes	,
T4231	5219-5226	NNS	denotes	minutes
T4230	5216-5218	CD	denotes	10
T4229	5210-5215	DT	denotes	every
T4228	5202-5209	NNS	denotes	seconds
T4227	5199-5201	CD	denotes	15
T4226	5195-5198	IN	denotes	for
T4225	5188-5194	NN	denotes	vortex
T4224	5185-5187	IN	denotes	on
T4223	5181-5184	VBN	denotes	put
T4222	5176-5180	VBD	denotes	were
T4221	5168-5175	NNS	denotes	pellets
T4220	5157-5167	VBG	denotes	containing
T4219	5151-5156	NNS	denotes	tubes
R1534	T1989	T1989	ROOT	2.1,2.1
R1535	T1990	T1989	punct	.,2.1
R1536	T1991	T2008	nsubjpass	Animals,provided
R1537	T1992	T1994	nummod	Ninety,rats
R1538	T1993	T1994	compound	Wister,rats
R1539	T1994	T2008	nsubjpass	rats,provided
R1540	T1995	T1994	punct	(,rats
R1541	T1996	T1999	amod	male,weight
R1542	T1997	T1999	punct	",",weight
R1543	T1998	T1999	compound	body,weight
R1544	T1999	T1994	appos	weight,rats
R1545	T2000	T1999	punct	:,weight
R1546	T2001	T2002	meta	250,g
R1547	T2002	T1999	appos	g,weight
R1548	T2003	T2002	prep	to,g
R1549	T2004	T2005	nummod	300,g
R1550	T2005	T2003	pobj	g,to
R1551	T2006	T1999	punct	),weight
R1552	T2007	T2008	auxpass	were,provided
R1553	T2008	T2008	ROOT	provided,provided
R1554	T2009	T2008	agent	by,provided
R1555	T2010	T2012	compound	Experimental,Center
R1556	T2011	T2012	compound	Animal,Center
R1557	T2012	T2009	pobj	Center,by
R1558	T2013	T2012	punct	",",Center
R1559	T2014	T2016	compound	China,University
R1560	T2015	T2016	compound	Medical,University
R1561	T2016	T2012	conj	University,Center
R1562	T2017	T2008	punct	.,provided
R1563	T2018	T2019	det	The,rats
R1564	T2019	T2021	nsubjpass	rats,assigned
R1565	T2020	T2021	auxpass	were,assigned
R1566	T2021	T2021	ROOT	assigned,assigned
R1567	T2022	T2021	advmod	randomly,assigned
R1568	T2023	T2021	prep	to,assigned
R1569	T2024	T2025	nummod	three,groups
R1570	T2025	T2023	pobj	groups,to
R1571	T2026	T2025	punct	:,groups
R1572	T2027	T2028	amod	normal,group
R1573	T2028	T2025	appos	group,groups
R1574	T2029	T2030	punct	(,N
R1575	T2030	T2028	appos	N,group
R1576	T2031	T2028	punct	",",group
R1577	T2032	T2052	dep	n,injection
R1578	T2033	T2034	punct	=,10
R1579	T2034	T2032	prep	10,n
R1580	T2035	T2032	punct	",",n
R1581	T2036	T2032	prep	without,n
R1582	T2037	T2038	det	any,treatment
R1583	T2038	T2036	pobj	treatment,without
R1584	T2039	T2032	punct	),n
R1585	T2040	T2032	punct	",",n
R1586	T2041	T2043	amod	normal,control
R1587	T2042	T2043	amod	saline,control
R1588	T2043	T2032	dobj	control,n
R1589	T2044	T2045	punct	(,S
R1590	T2045	T2052	dep	S,injection
R1591	T2046	T2052	punct	",",injection
R1592	T2047	T2052	nmod	n,injection
R1593	T2048	T2049	punct	=,40
R1594	T2049	T2047	prep	40,n
R1595	T2050	T2052	punct	",",injection
R1596	T2051	T2052	amod	intra-cerebral,injection
R1597	T2052	T2064	nmod	injection,ICH
R1598	T2053	T2052	prep	of,injection
R1599	T2054	T2055	amod	normal,saline
R1600	T2055	T2053	pobj	saline,of
R1601	T2056	T2052	prep	as,injection
R1602	T2057	T2056	pobj	control,as
R1603	T2058	T2052	punct	),injection
R1604	T2059	T2052	cc	and,injection
R1605	T2060	T2062	amod	intra-cerebral,group
R1606	T2061	T2062	compound	hemorrhage,group
R1607	T2062	T2052	conj	group,injection
R1608	T2063	T2064	punct	(,ICH
R1609	T2064	T2028	appos	ICH,group
R1610	T2065	T2064	punct	",",ICH
R1611	T2066	T2064	conj	n,ICH
R1612	T2067	T2068	punct	=,40
R1613	T2068	T2066	prep	40,n
R1614	T2069	T2066	punct	),n
R1615	T2070	T2021	punct	.,assigned
R1616	T2071	T2073	det	The,protocol
R1617	T2072	T2073	amod	experimental,protocol
R1618	T2073	T2078	nsubjpass	protocol,approved
R1619	T2074	T2073	acl	used,protocol
R1620	T2075	T2074	prep	for,used
R1621	T2076	T2075	pobj	animals,for
R1622	T2077	T2078	auxpass	was,approved
R1623	T2078	T2078	ROOT	approved,approved
R1624	T2079	T2078	agent	by,approved
R1625	T2080	T2085	det	the,Committee
R1626	T2081	T2082	compound	Animal,Care
R1627	T2082	T2085	nmod	Care,Committee
R1628	T2083	T2082	cc	and,Care
R1629	T2084	T2085	compound	Use,Committee
R1630	T2085	T2079	pobj	Committee,by
R1631	T2086	T2085	punct	",",Committee
R1632	T2087	T2089	compound	China,University
R1633	T2088	T2089	compound	Medical,University
R1634	T2089	T2085	conj	University,Committee
R1635	T2090	T2078	punct	.,approved
R1636	T2091	T2092	det	The,rats
R1637	T2092	T2094	nsubjpass	rats,subjected
R1638	T2093	T2094	auxpass	were,subjected
R1639	T2094	T2094	ROOT	subjected,subjected
R1640	T2095	T2094	prep	to,subjected
R1641	T2096	T2097	amod	intra-cerebral,injection
R1642	T2097	T2095	pobj	injection,to
R1643	T2098	T2097	prep	of,injection
R1644	T2099	T2100	amod	normal,saline
R1645	T2100	T2104	nmod	saline,blood
R1646	T2101	T2100	cc	or,saline
R1647	T2102	T2100	conj	autologous,saline
R1648	T2103	T2104	amod	whole,blood
R1649	T2104	T2098	pobj	blood,of
R1650	T2105	T2094	cc	and,subjected
R1651	T2106	T2107	auxpass	were,sacrificed
R1652	T2107	T2094	conj	sacrificed,subjected
R1653	T2108	T2107	prep	on,sacrificed
R1654	T2109	T2110	nummod	6,hours
R1655	T2110	T2108	pobj	hours,on
R1656	T2111	T2110	punct	",",hours
R1657	T2112	T2113	nummod	1,day
R1658	T2113	T2110	npadvmod	day,hours
R1659	T2114	T2110	punct	",",hours
R1660	T2115	T2116	nummod	3,days
R1661	T2116	T2110	appos	days,hours
R1662	T2117	T2116	cc	and,days
R1663	T2118	T2119	nummod	7,days
R1664	T2119	T2116	conj	days,days
R1665	T2120	T2119	prep	after,days
R1666	T2121	T2122	det	the,injection
R1667	T2122	T2120	pobj	injection,after
R1668	T2123	T2119	punct	",",days
R1669	T2124	T2116	advmod	respectively,days
R1670	T2125	T2094	punct	.,subjected
R1671	T2126	T2127	nummod	Five,rats
R1672	T2127	T2136	nsubjpass	rats,sacrificed
R1673	T2128	T2127	prep	in,rats
R1674	T2129	T2130	det	each,group
R1675	T2130	T2128	pobj	group,in
R1676	T2131	T2127	prep	at,rats
R1677	T2132	T2133	det	each,time
R1678	T2133	T2131	pobj	time,at
R1679	T2134	T2136	nsubjpass	point,sacrificed
R1680	T2135	T2136	auxpass	were,sacrificed
R1681	T2136	T2136	ROOT	sacrificed,sacrificed
R1682	T2137	T2136	agent	by,sacrificed
R1683	T2138	T2139	amod	transcardially,perfusion
R1684	T2139	T2137	pobj	perfusion,by
R1685	T2140	T2139	prep	of,perfusion
R1686	T2141	T2142	amod	normal,saline
R1687	T2142	T2140	pobj	saline,of
R1688	T2143	T2136	advcl	followed,sacrificed
R1689	T2144	T2143	agent	by,followed
R1690	T2145	T2146	nummod	4,%
R1691	T2146	T2144	pobj	%,by
R1692	T2147	T2136	conj	buffered,sacrificed
R1693	T2148	T2149	compound	Para,formaldehyde
R1694	T2149	T2147	dobj	formaldehyde,buffered
R1695	T2150	T2153	punct	(,7.4
R1696	T2151	T2153	quantmod	pH,7.4
R1697	T2152	T2153	punct	=,7.4
R1698	T2153	T2149	appos	7.4,formaldehyde
R1699	T2154	T2149	punct	),formaldehyde
R1700	T2155	T2136	punct	.,sacrificed
R1701	T2156	T2157	det	The,brains
R1702	T2157	T2159	nsubjpass	brains,removed
R1703	T2158	T2159	auxpass	were,removed
R1704	T2159	T2159	ROOT	removed,removed
R1705	T2160	T2159	punct	",",removed
R1706	T2161	T2159	conj	postfixed,removed
R1707	T2162	T2161	punct	",",postfixed
R1708	T2163	T2161	amod	embedded,postfixed
R1709	T2164	T2163	prep	in,embedded
R1710	T2165	T2164	pobj	paraffin,in
R1711	T2166	T2161	punct	",",postfixed
R1712	T2167	T2161	cc	and,postfixed
R1713	T2168	T2161	conj	cut,postfixed
R1714	T2169	T2168	prep	into,cut
R1715	T2170	T2169	pobj	sections,into
R1716	T2171	T2170	punct	(,sections
R1717	T2172	T2173	nummod	7,μm
R1718	T2173	T2170	appos	μm,sections
R1719	T2174	T2170	punct	),sections
R1720	T2175	T2176	mark	as,described
R1721	T2176	T2161	advcl	described,postfixed
R1722	T2177	T2178	advmod	previously,[
R1723	T2178	T2180	amod	[,]
R1724	T2179	T2180	nummod	8,]
R1725	T2180	T2176	dobj	],described
R1726	T2181	T2159	punct	.,removed
R1727	T2182	T2183	nummod	Five,rats
R1728	T2183	T2192	nsubjpass	rats,sacrificed
R1729	T2184	T2183	prep	in,rats
R1730	T2185	T2186	det	each,group
R1731	T2186	T2184	pobj	group,in
R1732	T2187	T2183	prep	at,rats
R1733	T2188	T2189	det	each,time
R1734	T2189	T2190	compound	time,point
R1735	T2190	T2187	pobj	point,at
R1736	T2191	T2192	auxpass	were,sacrificed
R1737	T2192	T2192	ROOT	sacrificed,sacrificed
R1738	T2193	T2192	agent	by,sacrificed
R1739	T2194	T2192	prep	over,sacrificed
R1740	T2195	T2196	compound	dose,injection
R1741	T2196	T2194	pobj	injection,over
R1742	T2197	T2196	prep	of,injection
R1743	T2198	T2197	pobj	Ketamine,of
R1744	T2199	T2192	punct	.,sacrificed
R1745	T2200	T2201	det	The,brains
R1746	T2201	T2203	nsubjpass	brains,removed
R1747	T2202	T2203	auxpass	were,removed
R1748	T2203	T2203	ROOT	removed,removed
R1749	T2204	T2203	cc	and,removed
R1750	T2205	T2203	conj	stored,removed
R1751	T2206	T2205	prep	at,stored
R1752	T2207	T2210	det	minus,C
R1753	T2208	T2209	nummod	80,°
R1754	T2209	T2210	nummod	°,C
R1755	T2210	T2205	npadvmod	C,stored
R1756	T2211	T2210	prep	for,C
R1757	T2212	T2211	pobj	RT-PCR,for
R1758	T2213	T2212	cc	and,RT-PCR
R1759	T2214	T2215	compound	Western,Blots
R1760	T2215	T2212	conj	Blots,RT-PCR
R1761	T2216	T2203	punct	.,removed
R1960	T2449	T2449	ROOT	2.2,2.2
R1961	T2450	T2449	punct	.,2.2
R1962	T2451	T2476	npadvmod	Induction,induced
R1963	T2452	T2451	prep	of,Induction
R1964	T2453	T2454	compound	Intracerebral,Hemorrhage
R1965	T2454	T2452	pobj	Hemorrhage,of
R1966	T2455	T2459	mark	Since,occur
R1967	T2456	T2458	amod	most,hemorrhages
R1968	T2457	T2458	amod	intracerebral,hemorrhages
R1969	T2458	T2459	nsubj	hemorrhages,occur
R1970	T2459	T2476	advcl	occur,induced
R1971	T2460	T2459	prep	in,occur
R1972	T2461	T2463	det	the,ganglia
R1973	T2462	T2463	amod	basal,ganglia
R1974	T2463	T2460	pobj	ganglia,in
R1975	T2464	T2459	advmod	clinically,occur
R1976	T2465	T2476	punct	",",induced
R1977	T2466	T2476	prep	in,induced
R1978	T2467	T2469	det	the,study
R1979	T2468	T2469	amod	present,study
R1980	T2469	T2466	pobj	study,in
R1981	T2470	T2469	punct	",",study
R1982	T2471	T2472	compound	rat,model
R1983	T2472	T2469	appos	model,study
R1984	T2473	T2472	prep	of,model
R1985	T2474	T2473	pobj	ICH,of
R1986	T2475	T2476	auxpass	was,induced
R1987	T2476	T2476	ROOT	induced,induced
R1988	T2477	T2476	prep	in,induced
R1989	T2478	T2480	amod	left,ganglia
R1990	T2479	T2480	amod	basal,ganglia
R1991	T2480	T2477	pobj	ganglia,in
R1992	T2481	T2476	punct	.,induced
R1993	T2482	T2483	nsubj	Rats,were
R1994	T2483	T2483	ROOT	were,were
R1995	T2484	T2483	acomp	anesthetized,were
R1996	T2485	T2484	prep	with,anesthetized
R1997	T2486	T2487	amod	intra-peritoneal,sodium
R1998	T2487	T2485	pobj	sodium,with
R1999	T2488	T2487	amod	pentobarbital,sodium
R2000	T2489	T2491	punct	(,mg/kg
R2001	T2490	T2491	nummod	60,mg/kg
R2002	T2491	T2488	appos	mg/kg,pentobarbital
R2003	T2492	T2491	punct	),mg/kg
R2004	T2493	T2483	punct	.,were
R2005	T2494	T2495	det	The,body
R2006	T2495	T2501	nsubjpass	body,maintained
R2007	T2496	T2495	punct	(,body
R2008	T2497	T2495	appos	rectal,body
R2009	T2498	T2495	punct	),body
R2010	T2499	T2501	nsubjpass	temperature,maintained
R2011	T2500	T2501	auxpass	was,maintained
R2012	T2501	T2501	ROOT	maintained,maintained
R2013	T2502	T2501	prep	at,maintained
R2014	T2503	T2504	nummod	37,°
R2015	T2504	T2505	nummod	°,C
R2016	T2505	T2502	pobj	C,at
R2017	T2506	T2501	prep	during,maintained
R2018	T2507	T2508	det	the,surgery
R2019	T2508	T2506	pobj	surgery,during
R2020	T2509	T2501	advcl	using,maintained
R2021	T2510	T2513	det	a,system
R2022	T2511	T2513	amod	feedback-controlled,system
R2023	T2512	T2513	compound	heating,system
R2024	T2513	T2509	dobj	system,using
R2025	T2514	T2501	punct	.,maintained
R2026	T2515	T2516	det	The,rats
R2027	T2516	T2518	nsubjpass	rats,positioned
R2028	T2517	T2518	auxpass	were,positioned
R2029	T2518	T2518	ROOT	positioned,positioned
R2030	T2519	T2518	prep	in,positioned
R2031	T2520	T2522	det	a,frame
R2032	T2521	T2522	compound	stereotactic,frame
R2033	T2522	T2519	pobj	frame,in
R2034	T2523	T2518	punct	",",positioned
R2035	T2524	T2518	cc	and,positioned
R2036	T2525	T2526	det	the,scalp
R2037	T2526	T2528	nsubjpass	scalp,incised
R2038	T2527	T2528	auxpass	was,incised
R2039	T2528	T2518	conj	incised,positioned
R2040	T2529	T2528	prep	along,incised
R2041	T2530	T2531	det	the,midline
R2042	T2531	T2529	pobj	midline,along
R2043	T2532	T2528	punct	.,incised
R2044	T2533	T2544	advcl	Using,opened
R2045	T2534	T2536	det	a,technique
R2046	T2535	T2536	amod	sterile,technique
R2047	T2536	T2533	dobj	technique,Using
R2048	T2537	T2544	punct	",",opened
R2049	T2538	T2542	det	a,hole
R2050	T2539	T2540	nummod	1,mm
R2051	T2540	T2542	compound	mm,hole
R2052	T2541	T2542	compound	burr,hole
R2053	T2542	T2544	nsubjpass	hole,opened
R2054	T2543	T2544	auxpass	was,opened
R2055	T2544	T2544	ROOT	opened,opened
R2056	T2545	T2544	prep	in,opened
R2057	T2546	T2547	det	the,skull
R2058	T2547	T2545	pobj	skull,in
R2059	T2548	T2544	prep	on,opened
R2060	T2549	T2552	det	the,suture
R2061	T2550	T2552	amod	left,suture
R2062	T2551	T2552	amod	coronal,suture
R2063	T2552	T2548	pobj	suture,on
R2064	T2553	T2554	nummod	3,mm
R2065	T2554	T2544	npadvmod	mm,opened
R2066	T2555	T2554	amod	lateral,mm
R2067	T2556	T2544	prep	to,opened
R2068	T2557	T2558	det	the,midline
R2069	T2558	T2556	pobj	midline,to
R2070	T2559	T2544	punct	.,opened
R2071	T2560	T2563	det	A,needle
R2072	T2561	T2563	amod	blunt,needle
R2073	T2562	T2563	amod	26-gauge,needle
R2074	T2563	T2565	nsubjpass	needle,inserted
R2075	T2564	T2565	auxpass	was,inserted
R2076	T2565	T2565	ROOT	inserted,inserted
R2077	T2566	T2565	prep	into,inserted
R2078	T2567	T2570	det	the,ganglia
R2079	T2568	T2570	amod	left,ganglia
R2080	T2569	T2570	amod	basal,ganglia
R2081	T2570	T2566	pobj	ganglia,into
R2082	T2571	T2570	prep	under,ganglia
R2083	T2572	T2573	amod	stereotactic,guidance
R2084	T2573	T2571	pobj	guidance,under
R2085	T2574	T2575	punct	(,coordinates
R2086	T2575	T2565	parataxis	coordinates,inserted
R2087	T2576	T2575	punct	:,coordinates
R2088	T2577	T2578	nummod	0.2,mm
R2089	T2578	T2579	compound	mm,anterior
R2090	T2579	T2575	dobj	anterior,coordinates
R2091	T2580	T2579	punct	",",anterior
R2092	T2581	T2582	nummod	6.0,mm
R2093	T2582	T2583	compound	mm,ventral
R2094	T2583	T2579	conj	ventral,anterior
R2095	T2584	T2583	punct	",",ventral
R2096	T2585	T2583	cc	and,ventral
R2097	T2586	T2587	nummod	3.0,mm
R2098	T2587	T2583	conj	mm,ventral
R2099	T2588	T2587	amod	lateral,mm
R2100	T2589	T2575	prep	to,coordinates
R2101	T2590	T2591	det	the,midline
R2102	T2591	T2589	pobj	midline,to
R2103	T2592	T2575	punct	),coordinates
R2104	T2593	T2565	punct	.,inserted
R2105	T2594	T2604	advmod	Then,infused
R2106	T2595	T2604	punct	",",infused
R2107	T2596	T2598	det	a,μL
R2108	T2597	T2598	nummod	75,μL
R2109	T2598	T2604	nsubjpass	μL,infused
R2110	T2599	T2598	prep	of,μL
R2111	T2600	T2602	amod	autologous,blood
R2112	T2601	T2602	amod	whole,blood
R2113	T2602	T2599	pobj	blood,of
R2114	T2603	T2604	auxpass	was,infused
R2115	T2604	T2604	ROOT	infused,infused
R2116	T2605	T2604	prep	at,infused
R2117	T2606	T2607	det	a,rate
R2118	T2607	T2605	pobj	rate,at
R2119	T2608	T2607	prep	of,rate
R2120	T2609	T2612	nummod	20,min
R2121	T2610	T2612	compound	μL,min
R2122	T2611	T2612	compound	/,min
R2123	T2612	T2608	pobj	min,of
R2124	T2613	T2604	prep	with,infused
R2125	T2614	T2615	det	the,use
R2126	T2615	T2613	pobj	use,with
R2127	T2616	T2615	prep	of,use
R2128	T2617	T2619	det	a,pump
R2129	T2618	T2619	amod	microinfusion,pump
R2130	T2619	T2616	pobj	pump,of
R2131	T2620	T2604	punct	.,infused
R2132	T2621	T2631	prep	After,withdrawn
R2133	T2622	T2623	det	the,completion
R2134	T2623	T2621	pobj	completion,After
R2135	T2624	T2623	prep	of,completion
R2136	T2625	T2626	det	the,infusion
R2137	T2626	T2624	pobj	infusion,of
R2138	T2627	T2631	punct	",",withdrawn
R2139	T2628	T2629	det	the,needle
R2140	T2629	T2631	nsubjpass	needle,withdrawn
R2141	T2630	T2631	auxpass	was,withdrawn
R2142	T2631	T2631	ROOT	withdrawn,withdrawn
R2143	T2632	T2631	advmod	quickly,withdrawn
R2144	T2633	T2631	punct	",",withdrawn
R2145	T2634	T2635	compound	cyanoacrylate,glue
R2146	T2635	T2637	nsubjpass	glue,placed
R2147	T2636	T2637	auxpass	was,placed
R2148	T2637	T2631	conj	placed,withdrawn
R2149	T2638	T2637	prep	around,placed
R2150	T2639	T2641	det	the,hole
R2151	T2640	T2641	compound	burr,hole
R2152	T2641	T2638	pobj	hole,around
R2153	T2642	T2637	punct	",",placed
R2154	T2643	T2637	cc	and,placed
R2155	T2644	T2646	det	the,incision
R2156	T2645	T2646	compound	skin,incision
R2157	T2646	T2647	nsubj	incision,was
R2158	T2647	T2637	conj	was,placed
R2159	T2648	T2647	acomp	closed,was
R2160	T2649	T2648	prep	with,closed
R2161	T2650	T2649	pobj	sutures,with
R2162	T2651	T2647	punct	.,was
R2163	T2652	T2665	prep	For,injected
R2164	T2653	T2657	det	the,rats
R2165	T2654	T2657	amod	normal,rats
R2166	T2655	T2656	amod	saline,control
R2167	T2656	T2657	compound	control,rats
R2168	T2657	T2652	pobj	rats,For
R2169	T2658	T2665	punct	",",injected
R2170	T2659	T2660	advmod	only,75
R2172	T2661	T2663	nmod	μL,saline
R2173	T2662	T2663	amod	normal,saline
R2174	T2663	T2665	nsubjpass	saline,injected
R2175	T2664	T2665	auxpass	was,injected
R2176	T2665	T2665	ROOT	injected,injected
R2177	T2666	T2665	punct	.,injected
R2436	T3069	T3069	ROOT	2.3,2.3
R2437	T3070	T3069	punct	.,2.3
R2438	T3071	T3086	advcl	Detection,isolated
R2439	T3072	T3071	prep	of,Detection
R2440	T3073	T3074	compound	TLR4,mRNA
R2441	T3074	T3072	pobj	mRNA,of
R2442	T3075	T3071	prep	by,Detection
R2443	T3076	T3077	det	the,Method
R2444	T3077	T3075	pobj	Method,by
R2445	T3078	T3077	prep	of,Method
R2446	T3079	T3080	compound	RT-PCR,Briefly
R2447	T3080	T3078	pobj	Briefly,of
R2448	T3081	T3086	punct	",",isolated
R2449	T3082	T3084	amod	total,samples
R2450	T3083	T3084	compound	RNA,samples
R2451	T3084	T3086	nsubjpass	samples,isolated
R2452	T3085	T3086	auxpass	were,isolated
R2453	T3086	T3086	ROOT	isolated,isolated
R2454	T3087	T3086	prep	from,isolated
R2455	T3088	T3089	amod	peri-hemorrhage,regions
R2456	T3089	T3087	pobj	regions,from
R2457	T3090	T3087	prep	in,from
R2458	T3091	T3093	amod	left,ganglia
R2459	T3092	T3093	amod	basal,ganglia
R2460	T3093	T3090	pobj	ganglia,in
R2461	T3094	T3086	advcl	using,isolated
R2462	T3095	T3100	advmod	Simply,Kit
R2463	T3096	T3100	nmod	P,Kit
R2464	T3097	T3100	compound	Total,Kit
R2465	T3098	T3099	compound	RNA,isolation
R2466	T3099	T3100	compound	isolation,Kit
R2467	T3100	T3094	dobj	Kit,using
R2468	T3101	T3102	punct	(,BioFlux
R2469	T3102	T3100	appos	BioFlux,Kit
R2470	T3103	T3102	punct	",",BioFlux
R2471	T3104	T3102	conj	HZ,BioFlux
R2472	T3105	T3104	punct	",",HZ
R2473	T3106	T3104	npadvmod	China,HZ
R2474	T3107	T3104	punct	),HZ
R2475	T3108	T3086	prep	according,isolated
R2476	T3109	T3108	prep	to,according
R2477	T3110	T3111	det	the,manufacturer
R2478	T3111	T3113	poss	manufacturer,instructions
R2479	T3112	T3111	case	's,manufacturer
R2480	T3113	T3109	pobj	instructions,to
R2481	T3114	T3086	punct	.,isolated
R2482	T3115	T3116	det	The,quantity
R2483	T3116	T3121	nsubjpass	quantity,determined
R2484	T3117	T3116	prep	of,quantity
R2485	T3118	T3119	amod	total,RNA
R2486	T3119	T3117	pobj	RNA,of
R2487	T3120	T3121	auxpass	was,determined
R2488	T3121	T3121	ROOT	determined,determined
R2489	T3122	T3121	prep	on,determined
R2490	T3123	T3124	det	a,Nanodrop
R2491	T3124	T3122	pobj	Nanodrop,on
R2492	T3125	T3124	punct	(,Nanodrop
R2493	T3126	T3127	compound	ND-1000,Spectrophotometer
R2494	T3127	T3124	appos	Spectrophotometer,Nanodrop
R2495	T3128	T3124	punct	),Nanodrop
R2496	T3129	T3121	prep	by,determined
R2497	T3130	T3129	pcomp	measuring,by
R2498	T3131	T3132	amod	optical,density
R2499	T3132	T3130	dobj	density,measuring
R2500	T3133	T3130	prep	at,measuring
R2501	T3134	T3133	pobj	A260,at
R2502	T3135	T3134	cc	and,A260
R2503	T3136	T3134	conj	A280,A260
R2504	T3137	T3138	compound	nm,wavelength
R2505	T3138	T3130	npadvmod	wavelength,measuring
R2506	T3139	T3121	punct	.,determined
R2507	T3140	T3147	advmod	Then,adjusted
R2508	T3141	T3147	punct	",",adjusted
R2509	T3142	T3143	det	the,concentrations
R2510	T3143	T3147	nsubjpass	concentrations,adjusted
R2511	T3144	T3143	prep	of,concentrations
R2512	T3145	T3144	pobj	mRNA,of
R2513	T3146	T3147	auxpass	were,adjusted
R2514	T3147	T3147	ROOT	adjusted,adjusted
R2515	T3148	T3147	prep	to,adjusted
R2516	T3149	T3150	nummod	1.0,g/mL
R2517	T3150	T3148	pobj	g/mL,to
R2518	T3151	T3147	punct	.,adjusted
R2519	T3152	T3154	nsubjpass	RNA,reverse-transcribed
R2520	T3153	T3154	auxpass	was,reverse-transcribed
R2521	T3154	T3154	ROOT	reverse-transcribed,reverse-transcribed
R2522	T3155	T3154	prep	into,reverse-transcribed
R2523	T3156	T3155	pobj	cDNA,into
R2524	T3157	T3154	prep	in,reverse-transcribed
R2525	T3158	T3161	nummod	20,system
R2526	T3159	T3161	compound	μL,system
R2527	T3160	T3161	compound	reaction,system
R2528	T3161	T3157	pobj	system,in
R2529	T3162	T3154	advcl	using,reverse-transcribed
R2530	T3163	T3166	compound	Superscript,Kit
R2531	T3164	T3166	compound	First-Strand,Kit
R2532	T3165	T3166	compound	Synthesis,Kit
R2533	T3166	T3162	dobj	Kit,using
R2534	T3167	T3162	prep	for,using
R2535	T3168	T3167	pobj	RT-PCR,for
R2536	T3169	T3171	punct	(,Inc
R2537	T3170	T3171	compound	promega,Inc
R2538	T3171	T3168	appos	Inc,RT-PCR
R2539	T3172	T3168	punct	),RT-PCR
R2540	T3173	T3162	prep	under,using
R2541	T3174	T3173	pobj	conditions,under
R2542	T3175	T3174	acl	described,conditions
R2543	T3176	T3175	agent	by,described
R2544	T3177	T3178	det	the,supplier
R2545	T3178	T3176	pobj	supplier,by
R2546	T3179	T3154	punct	.,reverse-transcribed
R2547	T3180	T3181	nummod	One,microliter
R2548	T3181	T3185	nsubjpass	microliter,used
R2549	T3182	T3181	prep	of,microliter
R2550	T3183	T3182	pobj	cDNA,of
R2551	T3184	T3185	auxpass	was,used
R2552	T3185	T3185	ROOT	used,used
R2553	T3186	T3187	aux	to,amplify
R2554	T3187	T3185	xcomp	amplify,used
R2555	T3188	T3191	det	the,fragment
R2556	T3189	T3191	nummod	TLR4,fragment
R2557	T3190	T3191	compound	gene,fragment
R2558	T3191	T3187	dobj	fragment,amplify
R2559	T3192	T3187	prep	with,amplify
R2560	T3193	T3196	nummod	1,buffer
R2561	T3194	T3196	nummod	×,buffer
R2562	T3195	T3196	compound	PCR,buffer
R2563	T3196	T3192	pobj	buffer,with
R2564	T3197	T3196	punct	",",buffer
R2565	T3198	T3200	nummod	1.5,MgCl2
R2566	T3199	T3200	compound	mM,MgCl2
R2567	T3200	T3196	appos	MgCl2,buffer
R2568	T3201	T3200	punct	",",MgCl2
R2569	T3202	T3203	nummod	200,μM
R2570	T3203	T3200	appos	μM,MgCl2
R2571	T3204	T3203	prep	of,μM
R2572	T3205	T3206	det	each,dNTP
R2573	T3206	T3204	pobj	dNTP,of
R2574	T3207	T3203	punct	",",μM
R2575	T3208	T3209	nummod	200,μM
R2576	T3209	T3203	conj	μM,μM
R2577	T3210	T3209	prep	of,μM
R2578	T3211	T3210	pobj	primers,of
R2579	T3212	T3209	punct	",",μM
R2580	T3213	T3209	cc	and,μM
R2581	T3214	T3217	nummod	2u,polymerase
R2582	T3215	T3217	compound	Taq,polymerase
R2583	T3216	T3217	compound	DNA,polymerase
R2584	T3217	T3209	conj	polymerase,μM
R2585	T3218	T3185	punct	.,used
R2586	T3219	T3220	det	The,house
R2587	T3220	T3222	compound	house,gene
R2588	T3221	T3222	compound	keep,gene
R2589	T3222	T3227	nsubjpass	gene,used
R2590	T3223	T3222	punct	(,gene
R2591	T3224	T3222	appos	β-actin,gene
R2592	T3225	T3222	punct	),gene
R2593	T3226	T3227	auxpass	was,used
R2594	T3227	T3227	ROOT	used,used
R2595	T3228	T3227	prep	as,used
R2596	T3229	T3231	det	an,control
R2597	T3230	T3231	amod	internal,control
R2598	T3231	T3228	pobj	control,as
R2599	T3232	T3227	punct	.,used
R2600	T3233	T3236	det	The,sequences
R2601	T3234	T3235	compound	PCR,primer
R2602	T3235	T3236	compound	primer,sequences
R2603	T3236	T3238	nsubjpass	sequences,designed
R2604	T3237	T3238	auxpass	were,designed
R2605	T3238	T3238	ROOT	designed,designed
R2606	T3239	T3238	prep	according,designed
R2607	T3240	T3239	prep	to,according
R2608	T3241	T3246	det	the,sequences
R2609	T3242	T3246	nummod	TLR4,sequences
R2610	T3243	T3242	cc	and,TLR4
R2611	T3244	T3242	conj	β-actin,TLR4
R2612	T3245	T3246	compound	gene,sequences
R2613	T3246	T3240	pobj	sequences,to
R2614	T3247	T3246	acl	reported,sequences
R2615	T3248	T3247	prep	in,reported
R2616	T3249	T3248	pobj	GenBank,in
R2617	T3250	T3238	punct	.,designed
R2618	T3251	T3251	ROOT	TLR4,TLR4
R2619	T3252	T3254	punct	(,bp
R2620	T3253	T3254	nummod	145,bp
R2621	T3254	T3251	appos	bp,TLR4
R2622	T3255	T3254	punct	),bp
R2623	T3256	T3254	punct	:,bp
R2624	T3257	T3263	advmod	forward,′
R2625	T3258	T3259	punct	:,5
R2626	T3259	T3263	nummod	5,′
R2627	T3260	T3259	punct	′,5
R2628	T3261	T3262	punct	-,AGCTTTGGTCAGTTGGCTCT-3
R2629	T3262	T3263	compound	AGCTTTGGTCAGTTGGCTCT-3,′
R2630	T3263	T3254	appos	′,bp
R2631	T3264	T3254	punct	;,bp
R2632	T3265	T3287	ccomp	reverse,reverse
R2633	T3266	T3265	punct	:,reverse
R2634	T3267	T3271	nummod	5,′
R2635	T3268	T3270	punct	′,CAGGATGACACCATTGAAGC-3
R2636	T3269	T3270	punct	-,CAGGATGACACCATTGAAGC-3
R2637	T3270	T3271	compound	CAGGATGACACCATTGAAGC-3,′
R2638	T3271	T3265	appos	′,reverse
R2639	T3272	T3287	punct	;,reverse
R2640	T3273	T3287	ccomp	β-actin,reverse
R2641	T3274	T3276	punct	(,bp
R2642	T3275	T3276	nummod	701,bp
R2643	T3276	T3273	appos	bp,β-actin
R2644	T3277	T3273	punct	),β-actin
R2645	T3278	T3273	punct	:,β-actin
R2646	T3279	T3273	advmod	forward,β-actin
R2647	T3280	T3273	punct	:,β-actin
R2648	T3281	T3284	meta	5,GCCAACCGTGAAAAAGATG-3
R2649	T3282	T3281	punct	′,5
R2650	T3283	T3284	punct	-,GCCAACCGTGAAAAAGATG-3
R2651	T3284	T3285	compound	GCCAACCGTGAAAAAGATG-3,′
R2652	T3285	T3273	appos	′,β-actin
R2653	T3286	T3287	punct	;,reverse
R2654	T3287	T3251	appos	reverse,TLR4
R2655	T3288	T3287	punct	:,reverse
R2656	T3289	T3292	nummod	5,CCAGGATAGAGCCACCAAT-3
R2657	T3290	T3292	punct	′,CCAGGATAGAGCCACCAAT-3
R2658	T3291	T3292	punct	-,CCAGGATAGAGCCACCAAT-3
R2659	T3292	T3293	compound	CCAGGATAGAGCCACCAAT-3,′
R2660	T3293	T3287	appos	′,reverse
R2661	T3294	T3287	punct	.,reverse
R2662	T3295	T3296	compound	Reaction,cycles
R2663	T3296	T3298	nsubjpass	cycles,performed
R2664	T3297	T3298	auxpass	were,performed
R2665	T3298	T3298	ROOT	performed,performed
R2666	T3299	T3298	prep	at,performed
R2667	T3300	T3302	det	the,condition
R2668	T3301	T3302	amod	following,condition
R2669	T3302	T3299	pobj	condition,at
R2670	T3303	T3298	punct	:,performed
R2671	T3304	T3314	nummod	94,s
R2672	T3305	T3306	compound	°,C
R2673	T3306	T3312	nmod	C,C
R2674	T3307	T3306	nummod	2,C
R2675	T3308	T3312	nmod	min,C
R2676	T3309	T3308	punct	",",min
R2677	T3310	T3311	nummod	94,°
R2678	T3311	T3312	compound	°,C
R2679	T3312	T3314	nmod	C,s
R2680	T3313	T3312	nummod	40,C
R2681	T3314	T3298	dep	s,performed
R2682	T3315	T3314	punct	",",s
R2683	T3316	T3327	nummod	55,s
R2684	T3317	T3318	compound	°,C
R2685	T3318	T3327	nmod	C,s
R2686	T3319	T3318	nummod	40,C
R2687	T3320	T3318	prep	s,C
R2688	T3321	T3318	punct	",",C
R2689	T3322	T3318	cc	and,C
R2690	T3323	T3318	conj	72,C
R2691	T3324	T3325	compound	°,C
R2692	T3325	T3327	nmod	C,s
R2693	T3326	T3325	nummod	60,C
R2694	T3327	T3314	appos	s,s
R2695	T3328	T3298	punct	.,performed
R2696	T3329	T3330	det	The,reaction
R2697	T3330	T3332	nsubjpass	reaction,stopped
R2698	T3331	T3332	auxpass	was,stopped
R2699	T3332	T3332	ROOT	stopped,stopped
R2700	T3333	T3332	prep	after,stopped
R2701	T3334	T3335	nummod	31,cycles
R2702	T3335	T3333	pobj	cycles,after
R2703	T3336	T3332	punct	.,stopped
R2704	T3337	T3338	compound	PCR,products
R2705	T3338	T3340	nsubjpass	products,electrophoresed
R2706	T3339	T3340	auxpass	were,electrophoresed
R2707	T3340	T3340	ROOT	electrophoresed,electrophoresed
R2708	T3341	T3340	prep	through,electrophoresed
R2709	T3342	T3343	amod	agarose,gel
R2710	T3343	T3341	pobj	gel,through
R2711	T3344	T3340	punct	.,electrophoresed
R2712	T3345	T3346	compound	Image,Acquisition
R2713	T3346	T3351	nsubjpass	Acquisition,used
R2714	T3347	T3346	cc	and,Acquisition
R2715	T3348	T3346	conj	Analysis,Acquisition
R2716	T3349	T3346	conj	software,Acquisition
R2717	T3350	T3351	auxpass	were,used
R2718	T3351	T3351	ROOT	used,used
R2719	T3352	T3353	aux	to,determine
R2720	T3353	T3351	xcomp	determine,used
R2721	T3354	T3355	compound	band,densities
R2722	T3355	T3353	dobj	densities,determine
R2723	T3356	T3351	punct	.,used
R2724	T3357	T3358	det	The,abundance
R2725	T3358	T3363	nsubjpass	abundance,expressed
R2726	T3359	T3358	prep	of,abundance
R2727	T3360	T3361	nummod	TLR4,mRNA
R2728	T3361	T3359	pobj	mRNA,of
R2729	T3362	T3363	auxpass	was,expressed
R2730	T3363	T3363	ROOT	expressed,expressed
R2731	T3364	T3363	prep	as,expressed
R2732	T3365	T3366	det	the,ratio
R2733	T3366	T3364	pobj	ratio,as
R2734	T3367	T3366	prep	of,ratio
R2735	T3368	T3369	nummod	TLR4,mRNA
R2736	T3369	T3367	pobj	mRNA,of
R2737	T3370	T3371	aux	to,β-actin
R2738	T3371	T3366	relcl	β-actin,ratio
R2739	T3372	T3371	dobj	mRNA,β-actin
R2740	T3373	T3363	punct	.,expressed
R2815	T3606	T3606	ROOT	2.4,2.4
R2816	T3607	T3606	punct	.,2.4
R2817	T3608	T3608	ROOT	Immunohistochemistry,Immunohistochemistry
R2818	T3609	T3610	punct	(,IHC
R2819	T3610	T3608	appos	IHC,Immunohistochemistry
R2820	T3611	T3608	punct	),Immunohistochemistry
R2821	T3612	T3613	det	The,IHC
R2822	T3613	T3618	nsubjpass	IHC,performed
R2823	T3614	T3613	acl	staining,IHC
R2824	T3615	T3614	prep	for,staining
R2825	T3616	T3615	pobj	TLR4,for
R2826	T3617	T3618	auxpass	was,performed
R2827	T3618	T3618	ROOT	performed,performed
R2828	T3619	T3620	mark	as,described
R2829	T3620	T3618	advcl	described,performed
R2830	T3621	T3622	advmod	previously,[
R2831	T3622	T3624	amod	[,]
R2832	T3623	T3624	nummod	8,]
R2833	T3624	T3620	dobj	],described
R2834	T3625	T3618	punct	.,performed
R2835	T3626	T3628	det	The,antibody
R2836	T3627	T3628	amod	primary,antibody
R2837	T3628	T3630	nsubj	antibody,was
R2838	T3629	T3628	acl	employed,antibody
R2839	T3630	T3630	ROOT	was,was
R2840	T3631	T3632	compound	rabbit,anti-TLR4
R2841	T3632	T3630	attr	anti-TLR4,was
R2842	T3633	T3636	punct	(,BA1717
R2843	T3634	T3636	amod	WuHan,BA1717
R2844	T3635	T3636	punct	",",BA1717
R2845	T3636	T3632	appos	BA1717,anti-TLR4
R2846	T3637	T3636	punct	),BA1717
R2847	T3638	T3630	punct	.,was
R2848	T3639	T3642	det	The,antibody
R2849	T3640	T3642	amod	biotinylated,antibody
R2850	T3641	T3642	amod	secondary,antibody
R2851	T3642	T3653	nsubjpass	antibody,obtained
R2852	T3643	T3642	cc	and,antibody
R2853	T3644	T3645	amod	antibody-biotin-avidin-peroxidase,complexes
R2854	T3645	T3642	conj	complexes,antibody
R2855	T3646	T3648	punct	(,reagent
R2856	T3647	T3648	compound	ABC,reagent
R2857	T3648	T3645	appos	reagent,complexes
R2858	T3649	T3648	punct	",",reagent
R2859	T3650	T3648	appos	SC-2018,reagent
R2860	T3651	T3648	punct	),reagent
R2861	T3652	T3653	auxpass	were,obtained
R2862	T3653	T3653	ROOT	obtained,obtained
R2863	T3654	T3653	prep	from,obtained
R2864	T3655	T3658	compound	Santa,Inc.
R2865	T3656	T3658	compound	Cruz,Inc.
R2866	T3657	T3658	compound	Biotechnology,Inc.
R2867	T3658	T3654	pobj	Inc.,from
R2868	T3659	T3658	punct	(,Inc.
R2869	T3660	T3661	compound	Santa,Cruz
R2870	T3661	T3658	npadvmod	Cruz,Inc.
R2871	T3662	T3661	punct	",",Cruz
R2872	T3663	T3661	appos	CA,Cruz
R2873	T3664	T3658	punct	),Inc.
R2874	T3665	T3653	punct	.,obtained
R2875	T3666	T3667	nsubj	Slides,processed
R2876	T3667	T3671	csubj	processed,served
R2877	T3668	T3667	prep	without,processed
R2878	T3669	T3670	amod	primary,antibodies
R2879	T3670	T3668	pobj	antibodies,without
R2880	T3671	T3671	ROOT	served,served
R2881	T3672	T3671	prep	as,served
R2882	T3673	T3674	amod	negative,controls
R2883	T3674	T3672	pobj	controls,as
R2884	T3675	T3671	punct	.,served
R2994	T3848	T3848	ROOT	2.5,2.5
R2995	T3849	T3848	punct	.,2.5
R2996	T3850	T3853	amod	Western,proteins
R2997	T3851	T3853	compound	Blots,proteins
R2998	T3852	T3853	compound	Cellular,proteins
R2999	T3853	T3855	nsubjpass	proteins,prepared
R3000	T3854	T3855	auxpass	were,prepared
R3001	T3855	T3855	ROOT	prepared,prepared
R3002	T3856	T3855	prep	from,prepared
R3003	T3857	T3859	amod	ICH,hemispheres
R3004	T3858	T3859	amod	cerebral,hemispheres
R3005	T3859	T3856	pobj	hemispheres,from
R3006	T3860	T3859	punct	",",hemispheres
R3007	T3861	T3859	acl	electrophoresed,hemispheres
R3008	T3862	T3861	prep	with,electrophoresed
R3009	T3863	T3864	amod	SDS-polyacrylamide,gel
R3010	T3864	T3862	pobj	gel,with
R3011	T3865	T3861	cc	and,electrophoresed
R3012	T3866	T3861	conj	transferred,electrophoresed
R3013	T3867	T3866	prep	onto,transferred
R3014	T3868	T3869	compound	Hybond,ECL
R3015	T3869	T3870	compound	ECL,membranes
R3016	T3870	T3867	pobj	membranes,onto
R3017	T3871	T3873	punct	(,Pharmacia
R3018	T3872	T3873	compound	Amersham,Pharmacia
R3019	T3873	T3870	appos	Pharmacia,membranes
R3020	T3874	T3873	punct	",",Pharmacia
R3021	T3875	T3873	conj	Piscataway,Pharmacia
R3022	T3876	T3875	punct	",",Piscataway
R3023	T3877	T3875	conj	NJ,Piscataway
R3024	T3878	T3870	punct	),membranes
R3025	T3879	T3855	punct	.,prepared
R3026	T3880	T3882	det	The,membranes
R3027	T3881	T3882	compound	ECL,membranes
R3028	T3882	T3884	nsubjpass	membranes,incubated
R3029	T3883	T3884	auxpass	were,incubated
R3030	T3884	T3884	ROOT	incubated,incubated
R3031	T3885	T3884	prep	with,incubated
R3032	T3886	T3889	det	the,antibody
R3033	T3887	T3889	amod	appropriate,antibody
R3034	T3888	T3889	amod	primary,antibody
R3035	T3889	T3885	pobj	antibody,with
R3036	T3890	T3889	punct	(,antibody
R3037	T3891	T3889	appos	WuHan,antibody
R3038	T3892	T3889	punct	",",antibody
R3039	T3893	T3889	appos	BA1717,antibody
R3040	T3894	T3884	punct	),incubated
R3041	T3895	T3884	advcl	followed,incubated
R3042	T3896	T3895	agent	by,followed
R3043	T3897	T3896	pobj	incubation,by
R3044	T3898	T3895	prep	with,followed
R3045	T3899	T3901	amod	peroxidase-conjugated,antibodies
R3066	T3920	T3916	punct	),system
R3067	T3921	T3912	punct	.,detected
R3068	T3922	T3924	det	The,membranes
R3069	T3923	T3924	amod	same,membranes
R3070	T3924	T3926	nsubjpass	membranes,probed
R3071	T3925	T3926	auxpass	were,probed
R3072	T3926	T3926	ROOT	probed,probed
R3073	T3927	T3926	prep	with,probed
R3074	T3928	T3927	pobj	anti-GAPDH,with
R3075	T3929	T3928	punct	(,anti-GAPDH
R3076	T3930	T3931	amod	glyceraldehyde-3-phosphate,dehydrogenase
R3077	T3931	T3928	appos	dehydrogenase,anti-GAPDH
R3078	T3932	T3931	punct	",",dehydrogenase
R3079	T3933	T3931	conj	Biodesign,dehydrogenase
R3080	T3934	T3933	punct	",",Biodesign
R3081	T3935	T3933	appos	Saco,Biodesign
R3082	T3936	T3935	punct	",",Saco
R3083	T3937	T3935	npadvmod	Maine,Saco
R3084	T3938	T3935	punct	),Saco
R3085	T3939	T3926	prep	after,probed
R3086	T3940	T3941	auxpass	being,washed
R3087	T3941	T3939	pcomp	washed,after
R3088	T3942	T3941	prep	with,washed
R3089	T3943	T3942	pcomp	stripping,with
R3090	T3944	T3943	dobj	buffer,stripping
R3091	T3945	T3926	punct	.,probed
R3092	T3946	T3947	det	The,signals
R3093	T3947	T3949	nsubjpass	signals,quantified
R3094	T3948	T3949	auxpass	were,quantified
R3095	T3949	T3949	ROOT	quantified,quantified
R3096	T3950	T3949	prep	by,quantified
R3097	T3951	T3950	pcomp	scanning,by
R3098	T3952	T3951	dobj	densitometry,scanning
R3099	T3953	T3952	cc	and,densitometry
R3100	T3954	T3956	amod	computer-assisted,analysis
R3101	T3955	T3956	compound	image,analysis
R3102	T3956	T3952	conj	analysis,densitometry
R3103	T3957	T3949	punct	.,quantified
R3278	T4204	T4206	punct	(,extract
R3279	T4205	T4206	nsubj	cytoplasmic,extract
R3280	T4206	T4206	ROOT	extract,extract
R3281	T4207	T4206	punct	),extract
R3282	T4208	T4212	punct	",",resuspended
R3283	T4209	T4210	det	the,pellets
R3284	T4210	T4212	nsubjpass	pellets,resuspended
R3285	T4211	T4212	auxpass	were,resuspended
R3286	T4212	T4212	ROOT	resuspended,resuspended
R3287	T4213	T4212	prep	in,resuspended
R3288	T4214	T4215	amod	nuclear,extraction
R3289	T4215	T4213	pobj	extraction,in
R3290	T4216	T4212	conj	reagent,resuspended
R3291	T4217	T4212	punct	.,resuspended
R3292	T4218	T4219	det	The,tubes
R3293	T4219	T4223	nsubjpass	tubes,put
R3294	T4220	T4219	acl	containing,tubes
R3295	T4221	T4220	dobj	pellets,containing
R3296	T4222	T4223	auxpass	were,put
R3297	T4223	T4223	ROOT	put,put
R3298	T4224	T4223	prep	on,put
R3299	T4225	T4224	pobj	vortex,on
R3300	T4226	T4223	prep	for,put
R3301	T4227	T4228	nummod	15,seconds
R3302	T4228	T4226	pobj	seconds,for
R3303	T4229	T4231	det	every,minutes
R3304	T4230	T4231	nummod	10,minutes
R3305	T4231	T4223	npadvmod	minutes,put
R3306	T4232	T4231	punct	",",minutes
R3307	T4233	T4231	prep	for,minutes
R3308	T4234	T4235	det	a,total
R3309	T4235	T4233	pobj	total,for
R3310	T4236	T4235	prep	of,total
R3311	T4237	T4238	nummod	40,minutes
R3312	T4238	T4236	pobj	minutes,of
R3313	T4239	T4223	punct	.,put
R3314	T4240	T4241	det	The,tubes
R3315	T4241	T4243	nsubjpass	tubes,centrifuged
R3316	T4242	T4243	auxpass	were,centrifuged
R3317	T4243	T4243	ROOT	centrifuged,centrifuged
R3318	T4244	T4243	prep	at,centrifuged
R3319	T4245	T4246	amod	maximum,speed
R3320	T4246	T4244	pobj	speed,at
R3321	T4247	T4251	punct	(,g
R3322	T4248	T4251	nmod	~,g
R3323	T4249	T4251	dep	"16,000",g
R3324	T4250	T4251	nummod	×,g
R3325	T4251	T4246	appos	g,speed
R3326	T4252	T4251	punct	),g
R3327	T4253	T4243	prep	in,centrifuged
R3328	T4254	T4256	det	a,centrifuge
R3329	T4255	T4256	amod	micro,centrifuge
R3330	T4256	T4253	pobj	centrifuge,in
R3331	T4257	T4243	prep	for,centrifuged
R3332	T4258	T4259	nummod	10,minutes
R3333	T4259	T4257	pobj	minutes,for
R3334	T4260	T4243	punct	.,centrifuged
R3335	T4261	T4262	det	The,supernatants
R3336	T4262	T4268	nsubjpass	supernatants,transferred
R3337	T4263	T4262	punct	(,supernatants
R3338	T4264	T4265	amod	nuclear,extract
R3339	T4265	T4262	appos	extract,supernatants
R3340	T4266	T4262	punct	),supernatants
R3341	T4267	T4268	auxpass	were,transferred
R3342	T4268	T4268	ROOT	transferred,transferred
R3343	T4269	T4268	prep	to,transferred
R3344	T4270	T4273	det	a,tube
R3345	T4271	T4273	amod	clean,tube
R3346	T4272	T4273	amod	prechilled,tube
R3347	T4273	T4269	pobj	tube,to
R3348	T4274	T4268	cc	and,transferred
R3349	T4275	T4268	conj	stored,transferred
R3350	T4276	T4275	prep	at,stored
R3351	T4277	T4279	nummod	−,°
R3352	T4278	T4279	nummod	80,°
R3353	T4279	T4276	pobj	°,at
R3354	T4280	T4275	npadvmod	C,stored
R3355	T4281	T4275	prep	until,stored
R3356	T4282	T4281	pobj	use,until
R3357	T4283	T4268	punct	.,transferred
R3358	T4284	T4286	nsubjpass	EMSA,performed
R3359	T4285	T4286	auxpass	was,performed
R3360	T4286	T4286	ROOT	performed,performed
R3361	T4287	T4288	mark	as,described
R3362	T4288	T4286	advcl	described,performed
R3363	T4289	T4290	advmod	previously,[
R3364	T4290	T4292	amod	[,]
R3365	T4291	T4292	nummod	8,]
R3366	T4292	T4288	dobj	],described
R3367	T4293	T4286	punct	.,performed
R3368	T4294	T4296	amod	NFκB,activity
R3369	T4295	T4296	amod	binding,activity
R3370	T4296	T4298	nsubjpass	activity,examined
R3371	T4297	T4298	auxpass	was,examined
R3372	T4298	T4298	ROOT	examined,examined
R3373	T4299	T4298	prep	in,examined
R3374	T4300	T4305	det	a,mixture
R3375	T4301	T4302	nummod	15,μL
R3376	T4302	T4305	compound	μL,mixture
R3377	T4303	T4304	amod	binding,reaction
R3378	T4304	T4305	compound	reaction,mixture
R3379	T4305	T4299	pobj	mixture,in
R3380	T4306	T4305	acl	containing,mixture
R3381	T4307	T4308	nummod	15,μg
R3382	T4308	T4317	nmod	μg,]
R3383	T4309	T4308	prep	of,μg
R3384	T4310	T4311	amod	nuclear,proteins
R3385	T4311	T4309	pobj	proteins,of
R3386	T4312	T4311	cc	and,proteins
R3387	T4313	T4314	nummod	35,fmol
R3388	T4314	T4317	compound	fmol,]
R3389	T4315	T4317	compound	[,]
R3390	T4316	T4317	compound	γ-32P,]
R3391	T4317	T4306	dobj	],containing
R3392	T4318	T4298	conj	labeled,examined
R3393	T4319	T4322	amod	double-stranded,oligonucleotide
R3394	T4320	T4321	compound	NFκB,consensus
R3395	T4321	T4322	compound	consensus,oligonucleotide
R3396	T4322	T4318	oprd	oligonucleotide,labeled
R3397	T4323	T4298	punct	.,examined
R3398	T4324	T4327	det	A,assay
R3399	T4325	T4326	amod	super,shift
R3400	T4326	T4327	compound	shift,assay
R3401	T4327	T4335	nsubjpass	assay,performed
R3402	T4328	T4327	acl	using,assay
R3403	T4329	T4328	dobj	antibodies,using
R3404	T4330	T4328	prep	to,using
R3405	T4331	T4330	pobj	P65,to
R3406	T4332	T4331	cc	and,P65
R3407	T4333	T4331	conj	P50,P65
R3408	T4334	T4335	auxpass	was,performed
R3409	T4335	T4335	ROOT	performed,performed
R3410	T4336	T4337	aux	to,confirm
R3411	T4337	T4335	xcomp	confirm,performed
R3412	T4338	T4340	nmod	NFκB,specificity
R3413	T4339	T4340	amod	binding,specificity
R3414	T4340	T4337	dobj	specificity,confirm
R3415	T4341	T4343	mark	as,described
R3416	T4342	T4343	advmod	previously,described
R3417	T4343	T4340	advcl	described,specificity
R3418	T4344	T4335	punct	.,performed
R3459	T4426	T4426	ROOT	2.7,2.7
R3460	T4427	T4426	punct	.,2.7
R3461	T4428	T4429	compound	Statistical,Analysis
R3462	T4429	T4430	compound	Analysis,All
R3463	T4430	T4431	compound	All,data
R3464	T4431	T4433	nsubjpass	data,presented
R3465	T4432	T4433	auxpass	were,presented
R3466	T4433	T4433	ROOT	presented,presented
R3467	T4434	T4433	prep	as,presented
R3468	T4435	T4437	amod	mean,SEM
R3469	T4436	T4437	compound	±,SEM
R3470	T4437	T4434	pobj	SEM,as
R3471	T4438	T4433	punct	.,presented
R3472	T4439	T4440	det	The,measurements
R3473	T4440	T4442	nsubjpass	measurements,analyzed
R3474	T4441	T4442	auxpass	were,analyzed
R3475	T4442	T4442	ROOT	analyzed,analyzed
R3476	T4443	T4442	agent	by,analyzed
R3477	T4444	T4445	amod	one-way,analysis
R3478	T4445	T4443	pobj	analysis,by
R3479	T4446	T4445	prep	of,analysis
R3480	T4447	T4446	pobj	variance,of
R3481	T4448	T4449	punct	(,ANOVA
R3482	T4449	T4447	appos	ANOVA,variance
R3483	T4450	T4449	punct	),ANOVA
R3484	T4451	T4447	cc	or,variance
R3485	T4452	T4454	poss	Student,t-test
R3486	T4453	T4452	case	's,Student
R3487	T4454	T4442	npadvmod	t-test,analyzed
R3488	T4455	T4442	punct	.,analyzed
R3489	T4456	T4460	det	The,level
R3490	T4457	T4460	nmod	P,level
R3491	T4458	T4460	nmod	<,level
R3492	T4459	T4458	nummod	.05,<
R3493	T4460	T4464	nsubjpass	level,used
R3494	T4461	T4460	prep	of,level
R3495	T4462	T4461	pobj	probability,of
R3496	T4463	T4464	auxpass	was,used
R3497	T4464	T4464	ROOT	used,used
R3498	T4465	T4464	prep	as,used
R3499	T4466	T4467	det	the,criteria
R3500	T4467	T4465	pobj	criteria,as
R3501	T4468	T4467	prep	of,criteria
R3502	T4469	T4468	pobj	significance,of
R3503	T4470	T4464	punct	.,used
R2171	T2660	T2663	nummod	75,saline
R3255	T4181	T4181	ROOT	2.6,2.6
R3256	T4182	T4181	punct	.,2.6
R3257	T4183	T4186	compound	Electrophoretic,Assay
R3258	T4184	T4185	compound	Mobility,Shift
R3259	T4185	T4186	compound	Shift,Assay
R3260	T4186	T4193	nsubjpass	Assay,isolated
R3261	T4187	T4188	punct	(,EMSA
R3262	T4188	T4186	appos	EMSA,Assay
R3263	T4189	T4188	punct	),EMSA
R3264	T4190	T4191	compound	Nuclear,proteins
R3265	T4191	T4192	nsubj	proteins,were
R3266	T4192	T4193	auxpass	were,isolated
R3267	T4193	T4193	ROOT	isolated,isolated
R3268	T4194	T4193	prep	from,isolated
R3269	T4195	T4196	compound	brain,tissue
R3270	T4196	T4194	pobj	tissue,from
R3271	T4197	T4193	punct	.,isolated
R3272	T4198	T4206	advmod	Briefly,extract
R3273	T4199	T4206	punct	",",extract
R3274	T4200	T4206	prep	after,extract
R3275	T4201	T4200	pobj	removal,after
R3276	T4202	T4201	prep	of,removal
R3277	T4203	T4202	pobj	supernatant,of
R3046	T3900	T3901	amod	secondary,antibodies
R3047	T3901	T3898	pobj	antibodies,with
R3048	T3902	T3901	punct	(,antibodies
R3049	T3903	T3904	compound	Golden,Bridge
R3050	T3904	T3901	appos	Bridge,antibodies
R3051	T3905	T3904	punct	",",Bridge
R3052	T3906	T3904	appos	PV-6001,Bridge
R3053	T3907	T3904	punct	),Bridge
R3054	T3908	T3884	punct	.,incubated
R3055	T3909	T3910	det	The,signals
R3056	T3910	T3912	nsubjpass	signals,detected
R3057	T3911	T3912	auxpass	were,detected
R3058	T3912	T3912	ROOT	detected,detected
R3059	T3913	T3912	prep	with,detected
R3060	T3914	T3916	det	the,system
R3061	T3915	T3916	compound	ECL,system
R3062	T3916	T3913	pobj	system,with
R3063	T3917	T3916	punct	(,system
R3064	T3918	T3919	compound	Amersham,Pharmacia
R3065	T3919	T3916	appos	Pharmacia,system

bionlp-st-ge-2016-test-tees

Id	Subject	Object	Predicate	Lexical cue
T3741	4727-4767	Protein	denotes	glyceraldehyde-3-phosphate dehydrogenase
T3740	4715-4725	Protein	denotes	anti-GAPDH
T3739	4542-4552	Protein	denotes	peroxidase
T2803	3767-3772	Positive_regulation	denotes	ratio
T2802	3723-3732	Positive_regulation	denotes	abundance
T2801	3789-3801	Protein	denotes	β-actin mRNA
T2800	3776-3785	Protein	denotes	TLR4 mRNA
T2799	3736-3745	Protein	denotes	TLR4 mRNA
T2798	3345-3352	Protein	denotes	β-actin
T2797	3247-3254	Protein	denotes	GenBank
T2796	3212-3219	Protein	denotes	β-actin
T2795	3203-3207	Protein	denotes	TLR4
T2794	3105-3112	Protein	denotes	β-actin
T2793	3064-3082	Protein	denotes	Taq DNA polymerase
T2792	2852-2872	Protein	denotes	RT-PCR (promega Inc)
T2791	2291-2300	Transcription	denotes	Detection
T2790	2304-2313	Protein	denotes	TLR4 mRNA
T4031	5770-5774	Protein	denotes	NFκB
T4030	5741-5744	Protein	denotes	P50
T4029	5733-5736	Protein	denotes	P65
T4028	5514-5521	Binding	denotes	binding
T4027	5645-5691	Protein	denotes	double-stranded NFκB consensus oligonucleotide
T4026	5509-5513	Protein	denotes	NFκB
T3538	4036-4046	Protein	denotes	peroxidase
T3537	4029-4035	Protein	denotes	avidin
T3536	3857-3861	Protein	denotes	TLR4
T2253	1306-1313	Positive_regulation	denotes	induced
T2252	1298-1301	Protein	denotes	ICH
T1775	901-909	Protein	denotes	paraffin
R2184	T2790	T2791	themeOf	TLR4 mRNA,Detection
R2185	T2799	T2802	themeOf	TLR4 mRNA,abundance
R2186	T2800	T2803	themeOf	TLR4 mRNA,ratio
R2187	T2801	T2803	causeOf	β-actin mRNA,ratio
R3108	T4026	T4028	themeOf	NFκB,binding
R1762	T2252	T2253	themeOf	ICH,induced

testone

Id	Subject	Object	Predicate	Lexical cue
T3995	5775-5782	Binding	denotes	binding
T3994	5741-5744	Protein	denotes	P50
T3993	5733-5736	Protein	denotes	P65
T3710	4727-4767	Protein	denotes	glyceraldehyde-3-phosphate dehydrogenase
T3709	4720-4725	Protein	denotes	GAPDH
T3507	4029-4035	Protein	denotes	avidin
T3506	3857-3861	Protein	denotes	TLR4
T2229	1306-1313	Positive_regulation	denotes	induced
T2703	3750-3759	Gene_expression	denotes	expressed
T2702	3789-3796	Protein	denotes	β-actin
T2701	3776-3780	Protein	denotes	TLR4
T2700	3736-3740	Protein	denotes	TLR4
T2699	3345-3352	Protein	denotes	β-actin
T2698	3256-3260	Protein	denotes	TLR4
T2697	3212-3219	Protein	denotes	β-actin
T2696	3203-3207	Protein	denotes	TLR4
T2695	3105-3112	Protein	denotes	β-actin
T2694	3084-3103	Protein	denotes	The house keep gene
T2693	3064-3082	Protein	denotes	Taq DNA polymerase
T2692	2964-2968	Protein	denotes	TLR4
T2691	2304-2308	Protein	denotes	TLR4
R2180	T2695	T2694	equivalentTo	β-actin,The house keep gene
R2181	T2701	T2703	themeOf	TLR4,expressed
R2886	T3710	T3709	equivalentTo	glyceraldehyde-3-phosphate dehydrogenase,GAPDH
R3104	T3993	T3995	themeOf	P65,binding
R3105	T3994	T3995	themeOf	P50,binding

test3

Id	Subject	Object	Predicate	Lexical cue
T4000	5775-5782	Binding	denotes	binding
T3999	5741-5744	Protein	denotes	P50
T3998	5733-5736	Protein	denotes	P65
T3997	5741-5744	Protein	denotes	P50
T3996	5733-5736	Protein	denotes	P65
T3714	4727-4767	Protein	denotes	glyceraldehyde-3-phosphate dehydrogenase
T3713	4720-4725	Protein	denotes	GAPDH
T3712	4727-4767	Protein	denotes	glyceraldehyde-3-phosphate dehydrogenase
T3711	4720-4725	Protein	denotes	GAPDH
T3511	4029-4035	Protein	denotes	avidin
T3510	3857-3861	Protein	denotes	TLR4
T3509	4029-4035	Protein	denotes	avidin
T3508	3857-3861	Protein	denotes	TLR4
T2230	1306-1313	Positive_regulation	denotes	induced
T2728	3789-3796	Protein	denotes	β-actin
T2727	3776-3780	Protein	denotes	TLR4
T2725	3736-3740	Protein	denotes	TLR4
T2724	3345-3352	Protein	denotes	β-actin
T2723	3256-3260	Protein	denotes	TLR4
T2722	3212-3219	Protein	denotes	β-actin
T2721	3203-3207	Protein	denotes	TLR4
T2720	3105-3112	Protein	denotes	β-actin
T2719	3084-3103	Protein	denotes	The house keep gene
T2718	3064-3082	Protein	denotes	Taq DNA polymerase
T2717	2964-2968	Protein	denotes	TLR4
T2716	2304-2308	Protein	denotes	TLR4
T2715	3789-3796	Protein	denotes	β-actin
T2714	3776-3780	Protein	denotes	TLR4
T2713	3736-3740	Protein	denotes	TLR4
T2712	3345-3352	Protein	denotes	β-actin
T2711	3256-3260	Protein	denotes	TLR4
T2710	3212-3219	Protein	denotes	β-actin
T2709	3203-3207	Protein	denotes	TLR4
T2708	3105-3112	Protein	denotes	β-actin
T2707	3084-3103	Protein	denotes	The house keep gene
T2706	3064-3082	Protein	denotes	Taq DNA polymerase
T2705	2964-2968	Protein	denotes	TLR4
T2704	2304-2308	Protein	denotes	TLR4
T2726	3750-3759	Transcription	denotes	expressed
R2182	T2720	T2719	equivalentTo	β-actin,The house keep gene
R2887	T3714	T3713	equivalentTo	glyceraldehyde-3-phosphate dehydrogenase,GAPDH
R3106	T3998	T4000	themeOf	P65,binding
R3107	T3999	T4000	themeOf	P50,binding

PMC:2817507 / 3530-9582 JSONTXT

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