PMC:2806624 / 54312-54409 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T26078	0-3	DT	denotes	The
T26079	4-9	NN	denotes	wells
T26080	10-14	VB	denotes	were
T26081	15-21	VB	denotes	washed
T26082	22-23	CD	denotes	4
T26083	24-29	NN	denotes	times
T26084	30-34	IN	denotes	with
T26085	35-39	NN	denotes	HKMG
T26086	40-46	NN	denotes	buffer
T26087	47-53	IN	denotes	before
T26088	54-60	VB	denotes	adding
T26089	61-64	DT	denotes	the
T26090	65-74	NN	denotes	substrate
T26091	75-82	NN	denotes	reagent
T26092	83-84	-LRB-	denotes	(
T26093	84-85	NN	denotes	R
T26094	85-86	CC	denotes	&
T26095	86-87	NNP	denotes	D
T26096	88-95	NNP	denotes	Systems
T26097	95-96	-RRB-	denotes	)
R20368	T26079	T26078	arg1Of	wells,The
R20369	T26079	T26080	arg1Of	wells,were
R20370	T26079	T26081	arg2Of	wells,washed
R20371	T26079	T26088	arg1Of	wells,adding
R20374	T26081	T26080	arg2Of	washed,were
R20375	T26081	T26087	arg1Of	washed,before
R20376	T26083	T26081	arg3Of	times,washed
R20377	T26083	T26082	arg1Of	times,4
R20378	T26083	T26084	arg1Of	times,with
R20379	T26086	T26084	arg2Of	buffer,with
R20380	T26086	T26085	arg1Of	buffer,HKMG
R20381	T26088	T26087	arg2Of	adding,before
R20382	T26091	T26088	arg2Of	reagent,adding
R20383	T26091	T26089	arg1Of	reagent,the
R20384	T26091	T26090	arg1Of	reagent,substrate
R20385	T26091	T26092	arg1Of	reagent,(
R20386	T26096	T26092	arg2Of	Systems,(
R20387	T26096	T26093	arg1Of	Systems,R
R20388	T26096	T26094	arg1Of	Systems,&
R20389	T26096	T26095	arg1Of	Systems,D
R20390	T26097	T26092	arg3Of	),(

Id	Subject	Object	Predicate	Lexical cue
T26384	0-3	DT	denotes	The
T26385	4-9	NNS	denotes	wells
T26386	10-14	VBD	denotes	were
T26387	15-21	VBN	denotes	washed
T26388	22-23	CD	denotes	4
T26389	24-29	NNS	denotes	times
T26390	30-34	IN	denotes	with
T26391	35-39	NNP	denotes	HKMG
T26392	40-46	NN	denotes	buffer
T26393	47-53	IN	denotes	before
T26394	54-60	VBG	denotes	adding
T26395	61-64	DT	denotes	the
T26396	65-74	JJ	denotes	substrate
T26397	75-82	NN	denotes	reagent
T26398	83-84	-LRB-	denotes	(
T26399	84-87	NNP	denotes	R&D
T26400	88-95	NNPS	denotes	Systems
T26401	95-96	-RRB-	denotes	)
T26402	96-97	.	denotes	.
R20658	T26384	T26385	det	The,wells
R20659	T26385	T26387	nsubjpass	wells,washed
R20660	T26386	T26387	auxpass	were,washed
R20661	T26387	T26387	ROOT	washed,washed
R20662	T26388	T26389	nummod	4,times
R20663	T26389	T26387	npadvmod	times,washed
R20664	T26390	T26387	prep	with,washed
R20665	T26391	T26392	compound	HKMG,buffer
R20666	T26392	T26390	pobj	buffer,with
R20667	T26393	T26387	prep	before,washed
R20668	T26394	T26393	pcomp	adding,before
R20669	T26395	T26397	det	the,reagent
R20670	T26396	T26397	compound	substrate,reagent
R20671	T26397	T26394	dobj	reagent,adding
R20672	T26398	T26400	punct	(,Systems
R20673	T26399	T26400	compound	R&D,Systems
R20674	T26400	T26397	appos	Systems,reagent
R20675	T26401	T26400	punct	),Systems
R20676	T26402	T26387	punct	.,washed

Id	Subject	Object	Predicate	Lexical cue
T372	0-97	Sentence	denotes	The wells were washed 4 times with HKMG buffer before adding the substrate reagent (R&D Systems).