PMC:2806624 / 45862-47129
Annnotations
pmc-enju-pas
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T22676 | 0-13 | NN | denotes | Transfections |
T22677 | 14-17 | CC | denotes | and |
T22678 | 18-26 | NN | denotes | reporter |
T22679 | 27-31 | NN | denotes | gene |
T22680 | 32-38 | NN | denotes | assays |
T22681 | 40-41 | NN | denotes | T |
T22682 | 42-47 | NN | denotes | cells |
T22683 | 48-52 | VB | denotes | were |
T22684 | 53-59 | VB | denotes | rested |
T22685 | 60-62 | IN | denotes | in |
T22686 | 63-73 | JJ | denotes | serum-free |
T22687 | 74-79 | NN | denotes | AIM-V |
T22688 | 80-86 | NN | denotes | medium |
T22689 | 87-96 | RB | denotes | overnight |
T22690 | 98-101 | CD | denotes | 3.5 |
T22691 | 102-104 | NN | denotes | µg |
T22692 | 105-107 | IN | denotes | of |
T22693 | 108-111 | DT | denotes | the |
T22694 | 112-117 | NN | denotes | FOXP3 |
T22695 | 118-126 | NN | denotes | promoter |
T22696 | 127-137 | NN | denotes | luciferase |
T22697 | 138-146 | NN | denotes | reporter |
T22698 | 147-153 | NN | denotes | vector |
T22699 | 154-156 | CC | denotes | or |
T22700 | 157-158 | DT | denotes | a |
T22701 | 159-170 | NN | denotes | combination |
T22702 | 171-179 | RB | denotes | together |
T22703 | 180-184 | IN | denotes | with |
T22704 | 185-188 | DT | denotes | the |
T22705 | 189-194 | NN | denotes | RUNX1 |
T22706 | 194-195 | -COMMA- | denotes | , |
T22707 | 196-201 | NN | denotes | RUNX3 |
T22708 | 202-209 | NN | denotes | pEGFPN1 |
T22709 | 210-216 | NN | denotes | vector |
T22710 | 216-217 | -COMMA- | denotes | , |
T22711 | 218-221 | CC | denotes | and |
T22712 | 222-225 | CD | denotes | 0.5 |
T22713 | 226-228 | NN | denotes | µg |
T22714 | 229-236 | NN | denotes | phRL-TK |
T22715 | 237-241 | VB | denotes | were |
T22716 | 242-247 | VB | denotes | added |
T22717 | 248-250 | TO | denotes | to |
T22718 | 251-252 | CD | denotes | 3 |
T22719 | 253-254 | SYM | denotes | × |
T22720 | 255-258 | CD | denotes | 106 |
T22721 | 259-263 | JJ | denotes | CD4+ |
T22722 | 264-265 | NN | denotes | T |
T22723 | 266-271 | NN | denotes | cells |
T22724 | 272-283 | VB | denotes | resuspended |
T22725 | 284-286 | IN | denotes | in |
T22726 | 287-290 | CD | denotes | 100 |
T22727 | 291-293 | NN | denotes | µl |
T22728 | 294-296 | IN | denotes | of |
T22729 | 297-309 | NN | denotes | Nucleofector |
T22730 | 310-318 | NN | denotes | solution |
T22731 | 319-320 | -LRB- | denotes | ( |
T22732 | 320-325 | NN | denotes | Lonza |
T22733 | 325-326 | -RRB- | denotes | ) |
T22734 | 327-330 | CC | denotes | and |
T22735 | 331-345 | VB | denotes | electroporated |
T22736 | 346-351 | VB | denotes | using |
T22737 | 352-355 | DT | denotes | the |
T22738 | 356-363 | NN | denotes | program |
T22739 | 364-368 | NN | denotes | U-15 |
T22740 | 370-375 | IN | denotes | After |
T22741 | 376-377 | DT | denotes | a |
T22742 | 378-382 | JJ | denotes | 24-h |
T22743 | 383-390 | NN | denotes | culture |
T22744 | 391-393 | IN | denotes | in |
T22745 | 394-404 | JJ | denotes | serum-free |
T22746 | 405-415 | NN | denotes | conditions |
T22747 | 416-419 | CC | denotes | and |
T22748 | 420-427 | NN | denotes | stimuli |
T22749 | 428-430 | IN | denotes | as |
T22750 | 431-440 | VB | denotes | indicated |
T22751 | 441-443 | IN | denotes | in |
T22752 | 444-447 | DT | denotes | the |
T22753 | 448-455 | NN | denotes | figures |
T22754 | 455-456 | -COMMA- | denotes | , |
T22755 | 457-467 | NN | denotes | luciferase |
T22756 | 468-476 | NN | denotes | activity |
T22757 | 477-480 | VB | denotes | was |
T22758 | 481-489 | VB | denotes | measured |
T22759 | 490-492 | IN | denotes | by |
T22760 | 493-496 | DT | denotes | the |
T22761 | 497-501 | JJ | denotes | dual |
T22762 | 502-512 | NN | denotes | luciferase |
T22763 | 513-518 | NN | denotes | assay |
T22764 | 519-525 | NN | denotes | system |
T22765 | 526-527 | -LRB- | denotes | ( |
T22766 | 527-534 | NN | denotes | Promega |
T22767 | 534-535 | -RRB- | denotes | ) |
T22768 | 536-545 | VB | denotes | according |
T22769 | 546-548 | TO | denotes | to |
T22770 | 549-552 | DT | denotes | the |
T22771 | 553-565 | NN | denotes | manufacturer |
T22772 | 565-567 | POS | denotes | 's |
T22773 | 568-580 | NN | denotes | instructions |
T22774 | 582-595 | NN | denotes | PMA/ionomycin |
T22775 | 596-599 | VB | denotes | was |
T22776 | 600-604 | VB | denotes | used |
T22777 | 605-607 | TO | denotes | to |
T22778 | 608-617 | VB | denotes | stimulate |
T22779 | 618-621 | DT | denotes | the |
T22780 | 622-627 | NN | denotes | cells |
T22781 | 627-628 | -COMMA- | denotes | , |
T22782 | 629-636 | IN | denotes | because |
T22783 | 637-640 | DT | denotes | the |
T22784 | 641-653 | NN | denotes | transfection |
T22785 | 654-657 | VB | denotes | was |
T22786 | 658-662 | RB | denotes | only |
T22787 | 663-672 | JJ | denotes | transient |
T22788 | 673-676 | CC | denotes | and |
T22789 | 677-680 | DT | denotes | the |
T22790 | 681-691 | NN | denotes | luciferase |
T22791 | 692-697 | NN | denotes | assay |
T22792 | 698-706 | VB | denotes | required |
T22793 | 707-708 | DT | denotes | a |
T22794 | 709-715 | JJ | denotes | strong |
T22795 | 716-719 | CC | denotes | and |
T22796 | 720-724 | JJ | denotes | fast |
T22797 | 725-736 | NN | denotes | stimulation |
T22798 | 737-739 | IN | denotes | of |
T22799 | 740-743 | DT | denotes | the |
T22800 | 744-749 | NN | denotes | cells |
T22801 | 751-753 | TO | denotes | To |
T22802 | 754-762 | VB | denotes | evaluate |
T22803 | 763-766 | DT | denotes | the |
T22804 | 767-773 | NN | denotes | effect |
T22805 | 774-776 | IN | denotes | of |
T22806 | 777-791 | NN | denotes | overexpression |
T22807 | 792-794 | IN | denotes | of |
T22808 | 795-800 | NN | denotes | RUNX1 |
T22809 | 801-803 | CC | denotes | or |
T22810 | 804-809 | NN | denotes | RUNX3 |
T22811 | 810-812 | IN | denotes | on |
T22812 | 813-818 | NN | denotes | FOXP3 |
T22813 | 819-826 | NN | denotes | protein |
T22814 | 827-833 | NN | denotes | levels |
T22815 | 833-834 | -COMMA- | denotes | , |
T22816 | 835-839 | JJ | denotes | CD4+ |
T22817 | 840-841 | NN | denotes | T |
T22818 | 842-847 | NN | denotes | cells |
T22819 | 848-852 | VB | denotes | were |
T22820 | 853-865 | VB | denotes | preactivated |
T22821 | 866-870 | IN | denotes | with |
T22822 | 871-872 | CD | denotes | 2 |
T22823 | 873-878 | NN | denotes | µg/ml |
T22824 | 879-897 | NN | denotes | phytohemagglutinin |
T22825 | 898-899 | -LRB- | denotes | ( |
T22826 | 899-912 | JJ | denotes | Sigma-Aldrich |
T22827 | 912-913 | -RRB- | denotes | ) |
T22828 | 914-916 | IN | denotes | in |
T22829 | 917-927 | JJ | denotes | serum-free |
T22830 | 928-933 | NN | denotes | AIM-V |
T22831 | 934-940 | NN | denotes | medium |
T22832 | 941-943 | IN | denotes | in |
T22833 | 944-947 | DT | denotes | the |
T22834 | 948-956 | NN | denotes | presence |
T22835 | 957-959 | IN | denotes | of |
T22836 | 960-961 | CD | denotes | 1 |
T22837 | 962-972 | NN | denotes | nmol/liter |
T22838 | 973-977 | NN | denotes | IL-2 |
T22839 | 978-979 | -LRB- | denotes | ( |
T22840 | 979-984 | NN | denotes | Roche |
T22841 | 984-985 | -RRB- | denotes | ) |
T22842 | 986-989 | IN | denotes | for |
T22843 | 990-992 | CD | denotes | 12 |
T22844 | 993-994 | NN | denotes | h |
T22845 | 994-995 | -COMMA- | denotes | , |
T22846 | 996-999 | CC | denotes | and |
T22847 | 1000-1004 | RB | denotes | then |
T22848 | 1005-1016 | VB | denotes | transfected |
T22849 | 1017-1021 | IN | denotes | with |
T22850 | 1022-1025 | DT | denotes | the |
T22851 | 1026-1032 | NN | denotes | vector |
T22852 | 1033-1040 | NN | denotes | pEGFPN1 |
T22853 | 1041-1051 | VB | denotes | containing |
T22854 | 1052-1055 | DT | denotes | the |
T22855 | 1056-1061 | NN | denotes | RUNX1 |
T22856 | 1062-1064 | CC | denotes | or |
T22857 | 1065-1070 | NN | denotes | RUNX3 |
T22858 | 1071-1079 | NN | denotes | fragment |
T22859 | 1080-1085 | VB | denotes | using |
T22860 | 1086-1089 | DT | denotes | the |
T22861 | 1090-1102 | NN | denotes | Nucleofector |
T22862 | 1103-1109 | NN | denotes | system |
T22863 | 1110-1111 | -LRB- | denotes | ( |
T22864 | 1111-1116 | NNP | denotes | Amaxa |
T22865 | 1117-1127 | NNP | denotes | Biosystems |
T22866 | 1127-1128 | -RRB- | denotes | ) |
T22867 | 1129-1132 | CC | denotes | and |
T22868 | 1133-1136 | DT | denotes | the |
T22869 | 1137-1144 | NN | denotes | program |
T22870 | 1145-1149 | NN | denotes | T-23 |
T22871 | 1151-1156 | NN | denotes | FOXP3 |
T22872 | 1157-1167 | NN | denotes | expression |
T22873 | 1168-1171 | VB | denotes | was |
T22874 | 1172-1181 | VB | denotes | evaluated |
T22875 | 1182-1184 | IN | denotes | by |
T22876 | 1185-1189 | NN | denotes | flow |
T22877 | 1190-1199 | NN | denotes | cytometry |
T22878 | 1200-1205 | IN | denotes | after |
T22879 | 1206-1208 | CD | denotes | 48 |
T22880 | 1209-1210 | NN | denotes | h |
T22881 | 1211-1213 | IN | denotes | of |
T22882 | 1214-1221 | NN | denotes | culture |
T22883 | 1222-1224 | IN | denotes | in |
T22884 | 1225-1230 | NN | denotes | AIM-V |
T22885 | 1231-1237 | NN | denotes | medium |
T22886 | 1238-1248 | VB | denotes | containing |
T22887 | 1249-1250 | CD | denotes | 1 |
T22888 | 1251-1261 | NN | denotes | nmol/liter |
T22889 | 1262-1266 | NN | denotes | IL-2 |
R17519 | T22676 | T22677 | arg1Of | Transfections,and |
R17520 | T22680 | T22677 | arg2Of | assays,and |
R17521 | T22680 | T22678 | arg1Of | assays,reporter |
R17522 | T22680 | T22679 | arg1Of | assays,gene |
R17523 | T22682 | T22681 | arg1Of | cells,T |
R17524 | T22682 | T22683 | arg1Of | cells,were |
R17525 | T22682 | T22684 | arg2Of | cells,rested |
R17526 | T22684 | T22683 | arg2Of | rested,were |
R17527 | T22684 | T22685 | arg1Of | rested,in |
R17528 | T22684 | T22689 | arg1Of | rested,overnight |
R17529 | T22688 | T22685 | arg2Of | medium,in |
R17530 | T22688 | T22686 | arg1Of | medium,serum-free |
R17531 | T22688 | T22687 | arg1Of | medium,AIM-V |
R17532 | T22691 | T22690 | arg1Of | µg,3.5 |
R17533 | T22691 | T22692 | arg1Of | µg,of |
R17534 | T22691 | T22699 | arg1Of | µg,or |
R17535 | T22698 | T22692 | arg2Of | vector,of |
R17536 | T22698 | T22693 | arg1Of | vector,the |
R17537 | T22698 | T22694 | arg1Of | vector,FOXP3 |
R17538 | T22698 | T22695 | arg1Of | vector,promoter |
R17539 | T22698 | T22696 | arg1Of | vector,luciferase |
R17540 | T22698 | T22697 | arg1Of | vector,reporter |
R17541 | T22699 | T22715 | arg1Of | or,were |
R17542 | T22699 | T22716 | arg2Of | or,added |
R17543 | T22699 | T22735 | arg2Of | or,electroporated |
R17544 | T22699 | T22736 | arg1Of | or,using |
R17545 | T22701 | T22702 | arg1Of | combination,together |
R17546 | T22701 | T22711 | arg1Of | combination,and |
R17547 | T22702 | T22703 | arg1Of | together,with |
R17548 | T22705 | T22704 | arg1Of | RUNX1,the |
R17549 | T22705 | T22706 | arg1Of | RUNX1,"," |
R17550 | T22706 | T22703 | arg2Of | ",",with |
R17551 | T22709 | T22706 | arg2Of | vector,"," |
R17552 | T22709 | T22707 | arg1Of | vector,RUNX3 |
R17553 | T22709 | T22708 | arg1Of | vector,pEGFPN1 |
R17554 | T22711 | T22699 | arg2Of | and,or |
R17555 | T22711 | T22700 | arg1Of | and,a |
R17556 | T22711 | T22710 | arg1Of | and,"," |
R17557 | T22714 | T22711 | arg2Of | phRL-TK,and |
R17558 | T22714 | T22712 | arg1Of | phRL-TK,0.5 |
R17559 | T22714 | T22713 | arg1Of | phRL-TK,µg |
R17560 | T22716 | T22717 | arg1Of | added,to |
R17561 | T22716 | T22734 | arg1Of | added,and |
R17562 | T22720 | T22719 | arg1Of | 106,× |
R17563 | T22723 | T22717 | arg2Of | cells,to |
R17564 | T22723 | T22718 | arg1Of | cells,3 |
R17565 | T22723 | T22720 | arg1Of | cells,106 |
R17566 | T22723 | T22721 | arg1Of | cells,CD4+ |
R17567 | T22723 | T22722 | arg1Of | cells,T |
R17568 | T22723 | T22724 | arg2Of | cells,resuspended |
R17569 | T22724 | T22725 | arg1Of | resuspended,in |
R17570 | T22727 | T22725 | arg2Of | µl,in |
R17571 | T22727 | T22726 | arg1Of | µl,100 |
R17572 | T22727 | T22728 | arg1Of | µl,of |
R17573 | T22730 | T22728 | arg2Of | solution,of |
R17574 | T22730 | T22729 | arg1Of | solution,Nucleofector |
R17575 | T22730 | T22731 | arg1Of | solution,( |
R17576 | T22732 | T22731 | arg2Of | Lonza,( |
R17577 | T22733 | T22731 | arg3Of | ),( |
R17578 | T22734 | T22715 | arg2Of | and,were |
R17579 | T22735 | T22734 | arg2Of | electroporated,and |
R17580 | T22736 | T22735 | arg3Of | using,electroporated |
R17581 | T22739 | T22736 | arg2Of | U-15,using |
R17582 | T22739 | T22737 | arg1Of | U-15,the |
R17583 | T22739 | T22738 | arg1Of | U-15,program |
R17584 | T22743 | T22740 | arg2Of | culture,After |
R17585 | T22743 | T22741 | arg1Of | culture,a |
R17586 | T22743 | T22742 | arg1Of | culture,24-h |
R17587 | T22743 | T22744 | arg1Of | culture,in |
R17588 | T22743 | T22749 | arg1Of | culture,as |
R17589 | T22746 | T22747 | arg1Of | conditions,and |
R17590 | T22747 | T22744 | arg2Of | and,in |
R17591 | T22747 | T22745 | arg1Of | and,serum-free |
R17592 | T22748 | T22747 | arg2Of | stimuli,and |
R17593 | T22750 | T22749 | arg2Of | indicated,as |
R17594 | T22750 | T22751 | arg1Of | indicated,in |
R17595 | T22753 | T22751 | arg2Of | figures,in |
R17596 | T22753 | T22752 | arg1Of | figures,the |
R17597 | T22756 | T22755 | arg1Of | activity,luciferase |
R17598 | T22756 | T22757 | arg1Of | activity,was |
R17599 | T22756 | T22758 | arg2Of | activity,measured |
R17600 | T22758 | T22740 | arg1Of | measured,After |
R17601 | T22758 | T22754 | arg1Of | measured,"," |
R17602 | T22758 | T22757 | arg2Of | measured,was |
R17603 | T22758 | T22768 | arg1Of | measured,according |
R17604 | T22764 | T22758 | arg1Of | system,measured |
R17605 | T22764 | T22759 | arg2Of | system,by |
R17606 | T22764 | T22760 | arg1Of | system,the |
R17607 | T22764 | T22761 | arg1Of | system,dual |
R17608 | T22764 | T22762 | arg1Of | system,luciferase |
R17609 | T22764 | T22763 | arg1Of | system,assay |
R17610 | T22764 | T22765 | arg1Of | system,( |
R17611 | T22766 | T22765 | arg2Of | Promega,( |
R17612 | T22767 | T22765 | arg3Of | ),( |
R17613 | T22769 | T22768 | arg2Of | to,according |
R17614 | T22771 | T22770 | arg1Of | manufacturer,the |
R17615 | T22771 | T22772 | arg2Of | manufacturer,'s |
R17616 | T22773 | T22769 | arg2Of | instructions,to |
R17617 | T22773 | T22772 | arg1Of | instructions,'s |
R17618 | T22774 | T22775 | arg1Of | PMA/ionomycin,was |
R17619 | T22774 | T22776 | arg2Of | PMA/ionomycin,used |
R17620 | T22776 | T22775 | arg2Of | used,was |
R17621 | T22776 | T22781 | arg1Of | used,"," |
R17622 | T22776 | T22782 | arg1Of | used,because |
R17623 | T22778 | T22776 | arg3Of | stimulate,used |
R17624 | T22778 | T22777 | arg1Of | stimulate,to |
R17625 | T22780 | T22778 | arg2Of | cells,stimulate |
R17626 | T22780 | T22779 | arg1Of | cells,the |
R17627 | T22784 | T22783 | arg1Of | transfection,the |
R17628 | T22784 | T22785 | arg1Of | transfection,was |
R17629 | T22784 | T22787 | arg1Of | transfection,transient |
R17630 | T22785 | T22788 | arg1Of | was,and |
R17631 | T22787 | T22785 | arg2Of | transient,was |
R17632 | T22787 | T22786 | arg1Of | transient,only |
R17633 | T22788 | T22782 | arg2Of | and,because |
R17634 | T22791 | T22789 | arg1Of | assay,the |
R17635 | T22791 | T22790 | arg1Of | assay,luciferase |
R17636 | T22791 | T22792 | arg1Of | assay,required |
R17637 | T22792 | T22788 | arg2Of | required,and |
R17638 | T22794 | T22795 | arg1Of | strong,and |
R17639 | T22796 | T22795 | arg2Of | fast,and |
R17640 | T22797 | T22792 | arg2Of | stimulation,required |
R17641 | T22797 | T22793 | arg1Of | stimulation,a |
R17642 | T22797 | T22794 | arg1Of | stimulation,strong |
R17643 | T22797 | T22796 | arg1Of | stimulation,fast |
R17644 | T22797 | T22798 | arg1Of | stimulation,of |
R17645 | T22800 | T22798 | arg2Of | cells,of |
R17646 | T22800 | T22799 | arg1Of | cells,the |
R17647 | T22802 | T22801 | arg1Of | evaluate,To |
R17648 | T22804 | T22802 | arg2Of | effect,evaluate |
R17649 | T22804 | T22803 | arg1Of | effect,the |
R17650 | T22804 | T22805 | arg1Of | effect,of |
R17651 | T22804 | T22811 | arg1Of | effect,on |
R17652 | T22806 | T22805 | arg2Of | overexpression,of |
R17653 | T22806 | T22807 | arg1Of | overexpression,of |
R17654 | T22808 | T22809 | arg1Of | RUNX1,or |
R17655 | T22809 | T22807 | arg2Of | or,of |
R17656 | T22810 | T22809 | arg2Of | RUNX3,or |
R17657 | T22814 | T22811 | arg2Of | levels,on |
R17658 | T22814 | T22812 | arg1Of | levels,FOXP3 |
R17659 | T22814 | T22813 | arg1Of | levels,protein |
R17660 | T22818 | T22802 | arg1Of | cells,evaluate |
R17661 | T22818 | T22816 | arg1Of | cells,CD4+ |
R17662 | T22818 | T22817 | arg1Of | cells,T |
R17663 | T22818 | T22819 | arg1Of | cells,were |
R17664 | T22818 | T22820 | arg2Of | cells,preactivated |
R17665 | T22818 | T22848 | arg2Of | cells,transfected |
R17666 | T22820 | T22821 | arg1Of | preactivated,with |
R17667 | T22820 | T22828 | arg1Of | preactivated,in |
R17668 | T22820 | T22832 | arg1Of | preactivated,in |
R17669 | T22820 | T22846 | arg1Of | preactivated,and |
R17670 | T22824 | T22821 | arg2Of | phytohemagglutinin,with |
R17671 | T22824 | T22822 | arg1Of | phytohemagglutinin,2 |
R17672 | T22824 | T22823 | arg1Of | phytohemagglutinin,µg/ml |
R17673 | T22824 | T22825 | arg1Of | phytohemagglutinin,( |
R17674 | T22826 | T22825 | arg2Of | Sigma-Aldrich,( |
R17675 | T22827 | T22825 | arg3Of | ),( |
R17676 | T22831 | T22828 | arg2Of | medium,in |
R17677 | T22831 | T22829 | arg1Of | medium,serum-free |
R17678 | T22831 | T22830 | arg1Of | medium,AIM-V |
R17679 | T22832 | T22833 | arg1Of | in,the |
R17680 | T22832 | T22835 | arg1Of | in,of |
R17681 | T22834 | T22832 | arg2Of | presence,in |
R17682 | T22838 | T22832 | arg3Of | IL-2,in |
R17683 | T22838 | T22836 | arg1Of | IL-2,1 |
R17684 | T22838 | T22837 | arg1Of | IL-2,nmol/liter |
R17685 | T22838 | T22839 | arg1Of | IL-2,( |
R17686 | T22838 | T22842 | arg1Of | IL-2,for |
R17687 | T22840 | T22839 | arg2Of | Roche,( |
R17688 | T22841 | T22839 | arg3Of | ),( |
R17689 | T22844 | T22842 | arg2Of | h,for |
R17690 | T22844 | T22843 | arg1Of | h,12 |
R17691 | T22846 | T22801 | modOf | and,To |
R17692 | T22846 | T22815 | arg1Of | and,"," |
R17693 | T22846 | T22819 | arg2Of | and,were |
R17694 | T22846 | T22845 | arg1Of | and,"," |
R17695 | T22848 | T22846 | arg2Of | transfected,and |
R17696 | T22848 | T22847 | arg1Of | transfected,then |
R17697 | T22848 | T22849 | arg1Of | transfected,with |
R17698 | T22852 | T22849 | arg2Of | pEGFPN1,with |
R17699 | T22852 | T22850 | arg1Of | pEGFPN1,the |
R17700 | T22852 | T22851 | arg1Of | pEGFPN1,vector |
R17701 | T22852 | T22853 | arg1Of | pEGFPN1,containing |
R17702 | T22855 | T22856 | arg1Of | RUNX1,or |
R17703 | T22857 | T22856 | arg2Of | RUNX3,or |
R17704 | T22858 | T22853 | arg2Of | fragment,containing |
R17705 | T22858 | T22854 | arg1Of | fragment,the |
R17706 | T22858 | T22855 | arg1Of | fragment,RUNX1 |
R17707 | T22858 | T22857 | arg1Of | fragment,RUNX3 |
R17708 | T22858 | T22859 | arg1Of | fragment,using |
R17709 | T22862 | T22860 | arg1Of | system,the |
R17710 | T22862 | T22861 | arg1Of | system,Nucleofector |
R17711 | T22862 | T22863 | arg1Of | system,( |
R17712 | T22862 | T22867 | arg1Of | system,and |
R17713 | T22865 | T22863 | arg2Of | Biosystems,( |
R17714 | T22865 | T22864 | arg1Of | Biosystems,Amaxa |
R17715 | T22866 | T22863 | arg3Of | ),( |
R17716 | T22867 | T22859 | arg2Of | and,using |
R17717 | T22870 | T22867 | arg2Of | T-23,and |
R17718 | T22870 | T22868 | arg1Of | T-23,the |
R17719 | T22870 | T22869 | arg1Of | T-23,program |
R17720 | T22872 | T22871 | arg1Of | expression,FOXP3 |
R17721 | T22872 | T22873 | arg1Of | expression,was |
R17722 | T22872 | T22874 | arg2Of | expression,evaluated |
R17723 | T22874 | T22873 | arg2Of | evaluated,was |
R17724 | T22874 | T22878 | arg1Of | evaluated,after |
R17725 | T22877 | T22874 | arg1Of | cytometry,evaluated |
R17726 | T22877 | T22875 | arg2Of | cytometry,by |
R17727 | T22877 | T22876 | arg1Of | cytometry,flow |
R17728 | T22880 | T22878 | arg2Of | h,after |
R17729 | T22880 | T22879 | arg1Of | h,48 |
R17730 | T22880 | T22881 | arg1Of | h,of |
R17731 | T22880 | T22883 | arg1Of | h,in |
R17732 | T22882 | T22881 | arg2Of | culture,of |
R17733 | T22885 | T22883 | arg2Of | medium,in |
R17734 | T22885 | T22884 | arg1Of | medium,AIM-V |
R17735 | T22885 | T22886 | arg1Of | medium,containing |
R17736 | T22889 | T22886 | arg2Of | IL-2,containing |
R17737 | T22889 | T22887 | arg1Of | IL-2,1 |
R17738 | T22889 | T22888 | arg1Of | IL-2,nmol/liter |
bionlp-st-ge-2016-spacy-parsed
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T22917 | 0-13 | NNS | denotes | Transfections |
T22918 | 14-17 | CC | denotes | and |
T22919 | 18-26 | NN | denotes | reporter |
T22920 | 27-31 | NN | denotes | gene |
T22921 | 32-38 | NNS | denotes | assays |
T22922 | 38-39 | . | denotes | . |
T22923 | 40-41 | NNP | denotes | T |
T22924 | 42-47 | NNS | denotes | cells |
T22925 | 48-52 | VBD | denotes | were |
T22926 | 53-59 | VBN | denotes | rested |
T22927 | 60-62 | IN | denotes | in |
T22928 | 63-73 | JJ | denotes | serum-free |
T22929 | 74-79 | NNP | denotes | AIM-V |
T22930 | 80-86 | NN | denotes | medium |
T22931 | 87-96 | JJ | denotes | overnight |
T22932 | 96-97 | . | denotes | . |
T22933 | 98-101 | CD | denotes | 3.5 |
T22934 | 102-104 | NN | denotes | µg |
T22935 | 105-107 | IN | denotes | of |
T22936 | 108-111 | DT | denotes | the |
T22937 | 112-117 | CD | denotes | FOXP3 |
T22938 | 118-126 | NN | denotes | promoter |
T22939 | 127-137 | NN | denotes | luciferase |
T22940 | 138-146 | NN | denotes | reporter |
T22941 | 147-153 | NN | denotes | vector |
T22942 | 154-156 | CC | denotes | or |
T22943 | 157-158 | DT | denotes | a |
T22944 | 159-170 | NN | denotes | combination |
T22945 | 171-179 | RB | denotes | together |
T22946 | 180-184 | IN | denotes | with |
T22947 | 185-188 | DT | denotes | the |
T22948 | 189-194 | NNP | denotes | RUNX1 |
T22949 | 194-195 | , | denotes | , |
T22950 | 196-201 | NNP | denotes | RUNX3 |
T22951 | 202-209 | NNP | denotes | pEGFPN1 |
T22952 | 210-216 | NN | denotes | vector |
T22953 | 216-217 | , | denotes | , |
T22954 | 218-221 | CC | denotes | and |
T22955 | 222-225 | CD | denotes | 0.5 |
T22956 | 226-228 | NN | denotes | µg |
T22957 | 229-236 | NN | denotes | phRL-TK |
T22958 | 237-241 | VBD | denotes | were |
T22959 | 242-247 | VBN | denotes | added |
T22960 | 248-250 | TO | denotes | to |
T22961 | 251-252 | CD | denotes | 3 |
T22962 | 253-254 | CD | denotes | × |
T22963 | 255-258 | CD | denotes | 106 |
T22964 | 259-262 | NNP | denotes | CD4 |
T22965 | 262-263 | CD | denotes | + |
T22966 | 264-265 | NNP | denotes | T |
T22967 | 266-271 | NNS | denotes | cells |
T22968 | 272-283 | VBD | denotes | resuspended |
T22969 | 284-286 | IN | denotes | in |
T22970 | 287-290 | CD | denotes | 100 |
T22971 | 291-293 | NN | denotes | µl |
T22972 | 294-296 | IN | denotes | of |
T22973 | 297-309 | NNP | denotes | Nucleofector |
T22974 | 310-318 | NN | denotes | solution |
T22975 | 319-320 | -LRB- | denotes | ( |
T22976 | 320-325 | NNP | denotes | Lonza |
T22977 | 325-326 | -RRB- | denotes | ) |
T22978 | 327-330 | CC | denotes | and |
T22979 | 331-345 | VBD | denotes | electroporated |
T22980 | 346-351 | VBG | denotes | using |
T22981 | 352-355 | DT | denotes | the |
T22982 | 356-363 | NN | denotes | program |
T22983 | 364-368 | NN | denotes | U-15 |
T22984 | 368-369 | . | denotes | . |
T22985 | 370-375 | IN | denotes | After |
T22986 | 376-377 | DT | denotes | a |
T22987 | 378-382 | JJ | denotes | 24-h |
T22988 | 383-390 | NN | denotes | culture |
T22989 | 391-393 | IN | denotes | in |
T22990 | 394-404 | JJ | denotes | serum-free |
T22991 | 405-415 | NNS | denotes | conditions |
T22992 | 416-419 | CC | denotes | and |
T22993 | 420-427 | NNS | denotes | stimuli |
T22994 | 428-430 | IN | denotes | as |
T22995 | 431-440 | VBN | denotes | indicated |
T22996 | 441-443 | IN | denotes | in |
T22997 | 444-447 | DT | denotes | the |
T22998 | 448-455 | NNS | denotes | figures |
T22999 | 455-456 | , | denotes | , |
T23000 | 457-467 | NN | denotes | luciferase |
T23001 | 468-476 | NN | denotes | activity |
T23002 | 477-480 | VBD | denotes | was |
T23003 | 481-489 | VBN | denotes | measured |
T23004 | 490-492 | IN | denotes | by |
T23005 | 493-496 | DT | denotes | the |
T23006 | 497-501 | JJ | denotes | dual |
T23007 | 502-512 | NN | denotes | luciferase |
T23008 | 513-518 | NN | denotes | assay |
T23009 | 519-525 | NN | denotes | system |
T23010 | 526-527 | -LRB- | denotes | ( |
T23011 | 527-534 | NNP | denotes | Promega |
T23012 | 534-535 | -RRB- | denotes | ) |
T23013 | 536-545 | VBG | denotes | according |
T23014 | 546-548 | TO | denotes | to |
T23015 | 549-552 | DT | denotes | the |
T23016 | 553-565 | NN | denotes | manufacturer |
T23017 | 565-567 | POS | denotes | 's |
T23018 | 568-580 | NNS | denotes | instructions |
T23019 | 580-581 | . | denotes | . |
T23020 | 582-595 | NNP | denotes | PMA/ionomycin |
T23021 | 596-599 | VBD | denotes | was |
T23022 | 600-604 | VBN | denotes | used |
T23023 | 605-607 | TO | denotes | to |
T23024 | 608-617 | VB | denotes | stimulate |
T23025 | 618-621 | DT | denotes | the |
T23026 | 622-627 | NNS | denotes | cells |
T23027 | 627-628 | , | denotes | , |
T23028 | 629-636 | IN | denotes | because |
T23029 | 637-640 | DT | denotes | the |
T23030 | 641-653 | NN | denotes | transfection |
T23031 | 654-657 | VBD | denotes | was |
T23032 | 658-662 | RB | denotes | only |
T23033 | 663-672 | JJ | denotes | transient |
T23034 | 673-676 | CC | denotes | and |
T23035 | 677-680 | DT | denotes | the |
T23036 | 681-691 | NN | denotes | luciferase |
T23037 | 692-697 | NN | denotes | assay |
T23038 | 698-706 | VBD | denotes | required |
T23039 | 707-708 | DT | denotes | a |
T23040 | 709-715 | JJ | denotes | strong |
T23041 | 716-719 | CC | denotes | and |
T23042 | 720-724 | JJ | denotes | fast |
T23043 | 725-736 | NN | denotes | stimulation |
T23044 | 737-739 | IN | denotes | of |
T23045 | 740-743 | DT | denotes | the |
T23046 | 744-749 | NNS | denotes | cells |
T23047 | 749-750 | . | denotes | . |
T23048 | 751-753 | TO | denotes | To |
T23049 | 754-762 | VB | denotes | evaluate |
T23050 | 763-766 | DT | denotes | the |
T23051 | 767-773 | NN | denotes | effect |
T23052 | 774-776 | IN | denotes | of |
T23053 | 777-791 | NN | denotes | overexpression |
T23054 | 792-794 | IN | denotes | of |
T23055 | 795-800 | CD | denotes | RUNX1 |
T23056 | 801-803 | CC | denotes | or |
T23057 | 804-809 | CD | denotes | RUNX3 |
T23058 | 810-812 | IN | denotes | on |
T23059 | 813-818 | CD | denotes | FOXP3 |
T23060 | 819-826 | NN | denotes | protein |
T23061 | 827-833 | NNS | denotes | levels |
T23062 | 833-834 | , | denotes | , |
T23063 | 835-838 | NNP | denotes | CD4 |
T23064 | 838-839 | NNP | denotes | + |
T23065 | 840-841 | NNP | denotes | T |
T23066 | 842-847 | NNS | denotes | cells |
T23067 | 848-852 | VBD | denotes | were |
T23068 | 853-865 | VBN | denotes | preactivated |
T23069 | 866-870 | IN | denotes | with |
T23070 | 871-872 | CD | denotes | 2 |
T23071 | 873-875 | NN | denotes | µg |
T23072 | 875-876 | NN | denotes | / |
T23073 | 876-878 | NN | denotes | ml |
T23074 | 879-897 | NN | denotes | phytohemagglutinin |
T23075 | 898-899 | -LRB- | denotes | ( |
T23076 | 899-912 | NNP | denotes | Sigma-Aldrich |
T23077 | 912-913 | -RRB- | denotes | ) |
T23078 | 914-916 | IN | denotes | in |
T23079 | 917-927 | JJ | denotes | serum-free |
T23080 | 928-933 | NNP | denotes | AIM-V |
T23081 | 934-940 | NN | denotes | medium |
T23082 | 941-943 | IN | denotes | in |
T23083 | 944-947 | DT | denotes | the |
T23084 | 948-956 | NN | denotes | presence |
T23085 | 957-959 | IN | denotes | of |
T23086 | 960-961 | CD | denotes | 1 |
T23087 | 962-972 | NN | denotes | nmol/liter |
T23088 | 973-977 | NN | denotes | IL-2 |
T23089 | 978-979 | -LRB- | denotes | ( |
T23090 | 979-984 | NNP | denotes | Roche |
T23091 | 984-985 | -RRB- | denotes | ) |
T23092 | 986-989 | IN | denotes | for |
T23093 | 990-992 | CD | denotes | 12 |
T23094 | 993-994 | NN | denotes | h |
T23095 | 994-995 | , | denotes | , |
T23096 | 996-999 | CC | denotes | and |
T23097 | 1000-1004 | RB | denotes | then |
T23098 | 1005-1016 | VBN | denotes | transfected |
T23099 | 1017-1021 | IN | denotes | with |
T23100 | 1022-1025 | DT | denotes | the |
T23101 | 1026-1032 | NN | denotes | vector |
T23102 | 1033-1040 | JJ | denotes | pEGFPN1 |
T23103 | 1041-1051 | VBG | denotes | containing |
T23104 | 1052-1055 | DT | denotes | the |
T23105 | 1056-1061 | CD | denotes | RUNX1 |
T23106 | 1062-1064 | CC | denotes | or |
T23107 | 1065-1070 | CD | denotes | RUNX3 |
T23108 | 1071-1079 | NN | denotes | fragment |
T23109 | 1080-1085 | VBG | denotes | using |
T23110 | 1086-1089 | DT | denotes | the |
T23111 | 1090-1102 | NNP | denotes | Nucleofector |
T23112 | 1103-1109 | NN | denotes | system |
T23113 | 1110-1111 | -LRB- | denotes | ( |
T23114 | 1111-1116 | NNP | denotes | Amaxa |
T23115 | 1117-1127 | NNPS | denotes | Biosystems |
T23116 | 1127-1128 | -RRB- | denotes | ) |
T23117 | 1129-1132 | CC | denotes | and |
T23118 | 1133-1136 | DT | denotes | the |
T23119 | 1137-1144 | NN | denotes | program |
T23120 | 1145-1149 | NN | denotes | T-23 |
T23121 | 1149-1150 | . | denotes | . |
T23122 | 1151-1156 | CD | denotes | FOXP3 |
T23123 | 1157-1167 | NN | denotes | expression |
T23124 | 1168-1171 | VBD | denotes | was |
T23125 | 1172-1181 | VBN | denotes | evaluated |
T23126 | 1182-1184 | IN | denotes | by |
T23127 | 1185-1189 | NN | denotes | flow |
T23128 | 1190-1199 | NN | denotes | cytometry |
T23129 | 1200-1205 | IN | denotes | after |
T23130 | 1206-1208 | CD | denotes | 48 |
T23131 | 1209-1210 | NN | denotes | h |
T23132 | 1211-1213 | IN | denotes | of |
T23133 | 1214-1221 | NN | denotes | culture |
T23134 | 1222-1224 | IN | denotes | in |
T23135 | 1225-1230 | NNP | denotes | AIM-V |
T23136 | 1231-1237 | NN | denotes | medium |
T23137 | 1238-1248 | VBG | denotes | containing |
T23138 | 1249-1250 | CD | denotes | 1 |
T23139 | 1251-1261 | NN | denotes | nmol/liter |
T23140 | 1262-1266 | NN | denotes | IL-2 |
T23141 | 1266-1267 | . | denotes | . |
R17739 | T22917 | T22917 | ROOT | Transfections,Transfections |
R17740 | T22918 | T22917 | cc | and,Transfections |
R17741 | T22919 | T22921 | compound | reporter,assays |
R17742 | T22920 | T22921 | compound | gene,assays |
R17743 | T22921 | T22917 | conj | assays,Transfections |
R17744 | T22922 | T22917 | punct | .,Transfections |
R17745 | T22923 | T22924 | compound | T,cells |
R17746 | T22924 | T22926 | nsubjpass | cells,rested |
R17747 | T22925 | T22926 | auxpass | were,rested |
R17748 | T22926 | T22926 | ROOT | rested,rested |
R17749 | T22927 | T22926 | prep | in,rested |
R17750 | T22928 | T22930 | amod | serum-free,medium |
R17751 | T22929 | T22930 | compound | AIM-V,medium |
R17752 | T22930 | T22927 | pobj | medium,in |
R17753 | T22931 | T22926 | advmod | overnight,rested |
R17754 | T22932 | T22926 | punct | .,rested |
R17755 | T22933 | T22934 | nummod | 3.5,µg |
R17763 | T22941 | T22935 | pobj | vector,of |
R17764 | T22942 | T22941 | cc | or,vector |
R17765 | T22943 | T22944 | det | a,combination |
R17766 | T22944 | T22934 | appos | combination,µg |
R17767 | T22945 | T22946 | advmod | together,with |
R17768 | T22946 | T22944 | prep | with,combination |
R17769 | T22947 | T22948 | det | the,RUNX1 |
R17770 | T22948 | T22946 | pobj | RUNX1,with |
R17771 | T22949 | T22948 | punct | ",",RUNX1 |
R17772 | T22950 | T22951 | compound | RUNX3,pEGFPN1 |
R17773 | T22951 | T22952 | compound | pEGFPN1,vector |
R17774 | T22952 | T22948 | conj | vector,RUNX1 |
R17775 | T22953 | T22952 | punct | ",",vector |
R17776 | T22954 | T22952 | cc | and,vector |
R17777 | T22955 | T22956 | nummod | 0.5,µg |
R17778 | T22956 | T22957 | compound | µg,phRL-TK |
R17779 | T22957 | T22952 | conj | phRL-TK,vector |
R17780 | T22958 | T22959 | auxpass | were,added |
R17781 | T22959 | T22959 | ROOT | added,added |
R17782 | T22960 | T22959 | prep | to,added |
R17783 | T22961 | T22967 | nummod | 3,cells |
R17784 | T22962 | T22967 | nummod | ×,cells |
R17785 | T22963 | T22967 | nummod | 106,cells |
R17786 | T22964 | T22967 | nmod | CD4,cells |
R17787 | T22965 | T22967 | nummod | +,cells |
R17788 | T22966 | T22967 | compound | T,cells |
R17789 | T22967 | T22960 | pobj | cells,to |
R17790 | T22968 | T22959 | oprd | resuspended,added |
R17791 | T22969 | T22968 | prep | in,resuspended |
R17792 | T22970 | T22971 | nummod | 100,µl |
R17793 | T22971 | T22969 | pobj | µl,in |
R17794 | T22972 | T22971 | prep | of,µl |
R17795 | T22973 | T22974 | compound | Nucleofector,solution |
R17796 | T22974 | T22972 | pobj | solution,of |
R17797 | T22975 | T22976 | punct | (,Lonza |
R17798 | T22976 | T22974 | appos | Lonza,solution |
R17799 | T22977 | T22976 | punct | ),Lonza |
R17800 | T22978 | T22968 | cc | and,resuspended |
R17801 | T22979 | T22968 | conj | electroporated,resuspended |
R17802 | T22980 | T22979 | xcomp | using,electroporated |
R17803 | T22981 | T22982 | det | the,program |
R17804 | T22982 | T22983 | compound | program,U-15 |
R17805 | T22983 | T22980 | dobj | U-15,using |
R17806 | T22984 | T22959 | punct | .,added |
R17807 | T22985 | T23003 | prep | After,measured |
R17808 | T22986 | T22988 | det | a,culture |
R17809 | T22987 | T22988 | amod | 24-h,culture |
R17810 | T22988 | T22985 | pobj | culture,After |
R17811 | T22989 | T22988 | prep | in,culture |
R17812 | T22990 | T22991 | amod | serum-free,conditions |
R17813 | T22991 | T22989 | pobj | conditions,in |
R17814 | T22992 | T22991 | cc | and,conditions |
R17815 | T22993 | T22991 | conj | stimuli,conditions |
R17816 | T22994 | T22995 | mark | as,indicated |
R17817 | T22995 | T23003 | advcl | indicated,measured |
R17818 | T22996 | T22995 | prep | in,indicated |
R17819 | T22997 | T22998 | det | the,figures |
R17820 | T22998 | T22996 | pobj | figures,in |
R17821 | T22999 | T23003 | punct | ",",measured |
R17822 | T23000 | T23001 | compound | luciferase,activity |
R17823 | T23001 | T23003 | nsubjpass | activity,measured |
R17824 | T23002 | T23003 | auxpass | was,measured |
R17825 | T23003 | T23003 | ROOT | measured,measured |
R17826 | T23004 | T23003 | agent | by,measured |
R17827 | T23005 | T23009 | det | the,system |
R17828 | T23006 | T23009 | amod | dual,system |
R17829 | T23007 | T23009 | compound | luciferase,system |
R17830 | T23008 | T23009 | compound | assay,system |
R17831 | T23009 | T23004 | pobj | system,by |
R17832 | T23010 | T23009 | punct | (,system |
R17833 | T23011 | T23009 | appos | Promega,system |
R17834 | T23012 | T23009 | punct | ),system |
R17835 | T23013 | T23003 | prep | according,measured |
R17836 | T23014 | T23013 | prep | to,according |
R17837 | T23015 | T23016 | det | the,manufacturer |
R17838 | T23016 | T23018 | poss | manufacturer,instructions |
R17839 | T23017 | T23016 | case | 's,manufacturer |
R17840 | T23018 | T23014 | pobj | instructions,to |
R17841 | T23019 | T23003 | punct | .,measured |
R17842 | T23020 | T23022 | nsubjpass | PMA/ionomycin,used |
R17843 | T23021 | T23022 | auxpass | was,used |
R17844 | T23022 | T23022 | ROOT | used,used |
R17845 | T23023 | T23024 | aux | to,stimulate |
R17846 | T23024 | T23022 | xcomp | stimulate,used |
R17847 | T23025 | T23026 | det | the,cells |
R17848 | T23026 | T23024 | dobj | cells,stimulate |
R17849 | T23027 | T23022 | punct | ",",used |
R17850 | T23028 | T23031 | mark | because,was |
R17851 | T23029 | T23030 | det | the,transfection |
R17852 | T23030 | T23031 | nsubj | transfection,was |
R17853 | T23031 | T23022 | advcl | was,used |
R17854 | T23032 | T23033 | advmod | only,transient |
R17855 | T23033 | T23031 | acomp | transient,was |
R17856 | T23034 | T23031 | cc | and,was |
R17857 | T23035 | T23037 | det | the,assay |
R17858 | T23036 | T23037 | compound | luciferase,assay |
R17859 | T23037 | T23038 | nsubj | assay,required |
R17860 | T23038 | T23031 | conj | required,was |
R17861 | T23039 | T23043 | det | a,stimulation |
R17862 | T23040 | T23043 | amod | strong,stimulation |
R17863 | T23041 | T23040 | cc | and,strong |
R17864 | T23042 | T23040 | conj | fast,strong |
R17865 | T23043 | T23038 | dobj | stimulation,required |
R17866 | T23044 | T23043 | prep | of,stimulation |
R17867 | T23045 | T23046 | det | the,cells |
R17868 | T23046 | T23044 | pobj | cells,of |
R17869 | T23047 | T23022 | punct | .,used |
R17870 | T23048 | T23049 | aux | To,evaluate |
R17871 | T23049 | T23068 | advcl | evaluate,preactivated |
R17872 | T23050 | T23051 | det | the,effect |
R17873 | T23051 | T23049 | dobj | effect,evaluate |
R17874 | T23052 | T23051 | prep | of,effect |
R17875 | T23053 | T23052 | pobj | overexpression,of |
R17876 | T23054 | T23053 | prep | of,overexpression |
R17877 | T23055 | T23054 | pobj | RUNX1,of |
R17878 | T23056 | T23055 | cc | or,RUNX1 |
R17879 | T23057 | T23055 | conj | RUNX3,RUNX1 |
R17880 | T23058 | T23057 | prep | on,RUNX3 |
R17881 | T23059 | T23061 | nummod | FOXP3,levels |
R17882 | T23060 | T23061 | compound | protein,levels |
R17883 | T23061 | T23058 | pobj | levels,on |
R17884 | T23062 | T23068 | punct | ",",preactivated |
R17885 | T23063 | T23066 | compound | CD4,cells |
R17886 | T23064 | T23066 | compound | +,cells |
R17887 | T23065 | T23066 | compound | T,cells |
R17888 | T23066 | T23068 | nsubjpass | cells,preactivated |
R17889 | T23067 | T23068 | auxpass | were,preactivated |
R17890 | T23068 | T23068 | ROOT | preactivated,preactivated |
R17891 | T23069 | T23068 | prep | with,preactivated |
R17892 | T23070 | T23071 | nummod | 2,µg |
R17893 | T23071 | T23074 | nmod | µg,phytohemagglutinin |
R17894 | T23072 | T23074 | nmod | /,phytohemagglutinin |
R17895 | T23073 | T23074 | compound | ml,phytohemagglutinin |
R17896 | T23074 | T23069 | pobj | phytohemagglutinin,with |
R17897 | T23075 | T23074 | punct | (,phytohemagglutinin |
R17898 | T23076 | T23074 | appos | Sigma-Aldrich,phytohemagglutinin |
R17899 | T23077 | T23074 | punct | ),phytohemagglutinin |
R17900 | T23078 | T23074 | prep | in,phytohemagglutinin |
R17901 | T23079 | T23081 | amod | serum-free,medium |
R17902 | T23080 | T23081 | compound | AIM-V,medium |
R17903 | T23081 | T23078 | pobj | medium,in |
R17904 | T23082 | T23074 | prep | in,phytohemagglutinin |
R17905 | T23083 | T23084 | det | the,presence |
R17906 | T23084 | T23082 | pobj | presence,in |
R17907 | T23085 | T23084 | prep | of,presence |
R17908 | T23086 | T23087 | nummod | 1,nmol/liter |
R17909 | T23087 | T23088 | compound | nmol/liter,IL-2 |
R17910 | T23088 | T23085 | pobj | IL-2,of |
R17911 | T23089 | T23084 | punct | (,presence |
R17912 | T23090 | T23084 | appos | Roche,presence |
R17913 | T23091 | T23084 | punct | ),presence |
R17914 | T23092 | T23074 | prep | for,phytohemagglutinin |
R17949 | T23127 | T23126 | pobj | flow,by |
R17950 | T23128 | T23125 | npadvmod | cytometry,evaluated |
R17951 | T23129 | T23128 | prep | after,cytometry |
R17952 | T23130 | T23131 | nummod | 48,h |
R17953 | T23131 | T23129 | pobj | h,after |
R17954 | T23132 | T23131 | prep | of,h |
R17955 | T23133 | T23132 | pobj | culture,of |
R17956 | T23134 | T23131 | prep | in,h |
R17957 | T23135 | T23136 | compound | AIM-V,medium |
R17958 | T23136 | T23134 | pobj | medium,in |
R17959 | T23137 | T23131 | acl | containing,h |
R17960 | T23138 | T23139 | nummod | 1,nmol/liter |
R17961 | T23139 | T23140 | compound | nmol/liter,IL-2 |
R17962 | T23140 | T23137 | dobj | IL-2,containing |
R17963 | T23141 | T23125 | punct | .,evaluated |
R17756 | T22934 | T22959 | nsubjpass | µg,added |
R17757 | T22935 | T22934 | prep | of,µg |
R17758 | T22936 | T22941 | det | the,vector |
R17759 | T22937 | T22941 | nummod | FOXP3,vector |
R17760 | T22938 | T22941 | nmod | promoter,vector |
R17761 | T22939 | T22941 | compound | luciferase,vector |
R17762 | T22940 | T22941 | compound | reporter,vector |
R17915 | T23093 | T23094 | nummod | 12,h |
R17916 | T23094 | T23092 | pobj | h,for |
R17917 | T23095 | T23074 | punct | ",",phytohemagglutinin |
R17918 | T23096 | T23074 | cc | and,phytohemagglutinin |
R17919 | T23097 | T23098 | advmod | then,transfected |
R17920 | T23098 | T23068 | conj | transfected,preactivated |
R17921 | T23099 | T23098 | prep | with,transfected |
R17922 | T23100 | T23102 | det | the,pEGFPN1 |
R17923 | T23101 | T23102 | compound | vector,pEGFPN1 |
R17924 | T23102 | T23099 | pobj | pEGFPN1,with |
R17925 | T23103 | T23102 | prep | containing,pEGFPN1 |
R17926 | T23104 | T23105 | det | the,RUNX1 |
R17927 | T23105 | T23108 | nummod | RUNX1,fragment |
R17928 | T23106 | T23105 | cc | or,RUNX1 |
R17929 | T23107 | T23108 | nummod | RUNX3,fragment |
R17930 | T23108 | T23103 | dobj | fragment,containing |
R17931 | T23109 | T23108 | acl | using,fragment |
R17932 | T23110 | T23112 | det | the,system |
R17933 | T23111 | T23112 | compound | Nucleofector,system |
R17934 | T23112 | T23109 | dobj | system,using |
R17935 | T23113 | T23112 | punct | (,system |
R17936 | T23114 | T23115 | compound | Amaxa,Biosystems |
R17937 | T23115 | T23112 | appos | Biosystems,system |
R17938 | T23116 | T23112 | punct | ),system |
R17939 | T23117 | T23108 | cc | and,fragment |
R17940 | T23118 | T23120 | det | the,T-23 |
R17941 | T23119 | T23120 | compound | program,T-23 |
R17942 | T23120 | T23108 | conj | T-23,fragment |
R17943 | T23121 | T23068 | punct | .,preactivated |
R17944 | T23122 | T23123 | nummod | FOXP3,expression |
R17945 | T23123 | T23125 | nsubjpass | expression,evaluated |
R17946 | T23124 | T23125 | auxpass | was,evaluated |
R17947 | T23125 | T23125 | ROOT | evaluated,evaluated |
R17948 | T23126 | T23125 | agent | by,evaluated |
UBERON-AE
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T22641 | 63-68 | http://purl.obolibrary.org/obo/UBERON_0001977 | denotes | serum |
T22642 | 394-399 | http://purl.obolibrary.org/obo/UBERON_0001977 | denotes | serum |
T22643 | 917-922 | http://purl.obolibrary.org/obo/UBERON_0001977 | denotes | serum |
GO-BP
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T23142 | 457-476 | http://purl.obolibrary.org/obo/GO_0045289 | denotes | luciferase activity |
T23143 | 457-476 | http://purl.obolibrary.org/obo/GO_0047077 | denotes | luciferase activity |
T23144 | 457-476 | http://purl.obolibrary.org/obo/GO_0047712 | denotes | luciferase activity |
T23145 | 457-476 | http://purl.obolibrary.org/obo/GO_0050248 | denotes | luciferase activity |
T23146 | 457-476 | http://purl.obolibrary.org/obo/GO_0050397 | denotes | luciferase activity |
GO-MF
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T23172 | 457-476 | http://purl.obolibrary.org/obo/GO_0045289 | denotes | luciferase activity |
T23173 | 457-476 | http://purl.obolibrary.org/obo/GO_0047077 | denotes | luciferase activity |
T23174 | 457-476 | http://purl.obolibrary.org/obo/GO_0047712 | denotes | luciferase activity |
T23175 | 457-476 | http://purl.obolibrary.org/obo/GO_0050248 | denotes | luciferase activity |
T23176 | 457-476 | http://purl.obolibrary.org/obo/GO_0050397 | denotes | luciferase activity |
T23177 | 973-977 | http://purl.obolibrary.org/obo/GO_0005134 | denotes | IL-2 |
T23178 | 1262-1266 | http://purl.obolibrary.org/obo/GO_0005134 | denotes | IL-2 |
GO-CC
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T23179 | 42-47 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T23180 | 266-271 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T23181 | 622-627 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T23182 | 744-749 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T23183 | 842-847 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T22669 | 0-39 | Sentence | denotes | Transfections and reporter gene assays. |
T22670 | 40-97 | Sentence | denotes | T cells were rested in serum-free AIM-V medium overnight. |
T22671 | 98-369 | Sentence | denotes | 3.5 µg of the FOXP3 promoter luciferase reporter vector or a combination together with the RUNX1, RUNX3 pEGFPN1 vector, and 0.5 µg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 µl of Nucleofector solution (Lonza) and electroporated using the program U-15. |
T22672 | 370-581 | Sentence | denotes | After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega) according to the manufacturer's instructions. |
T22673 | 582-750 | Sentence | denotes | PMA/ionomycin was used to stimulate the cells, because the transfection was only transient and the luciferase assay required a strong and fast stimulation of the cells. |
T22674 | 751-1150 | Sentence | denotes | To evaluate the effect of overexpression of RUNX1 or RUNX3 on FOXP3 protein levels, CD4+ T cells were preactivated with 2 µg/ml phytohemagglutinin (Sigma-Aldrich) in serum-free AIM-V medium in the presence of 1 nmol/liter IL-2 (Roche) for 12 h, and then transfected with the vector pEGFPN1 containing the RUNX1 or RUNX3 fragment using the Nucleofector system (Amaxa Biosystems) and the program T-23. |
T22675 | 1151-1267 | Sentence | denotes | FOXP3 expression was evaluated by flow cytometry after 48 h of culture in AIM-V medium containing 1 nmol/liter IL-2. |
T315 | 0-39 | Sentence | denotes | Transfections and reporter gene assays. |
T316 | 40-97 | Sentence | denotes | T cells were rested in serum-free AIM-V medium overnight. |
T317 | 98-369 | Sentence | denotes | 3.5 µg of the FOXP3 promoter luciferase reporter vector or a combination together with the RUNX1, RUNX3 pEGFPN1 vector, and 0.5 µg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 µl of Nucleofector solution (Lonza) and electroporated using the program U-15. |
T318 | 370-581 | Sentence | denotes | After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega) according to the manufacturer's instructions. |
T319 | 582-750 | Sentence | denotes | PMA/ionomycin was used to stimulate the cells, because the transfection was only transient and the luciferase assay required a strong and fast stimulation of the cells. |
T320 | 751-1150 | Sentence | denotes | To evaluate the effect of overexpression of RUNX1 or RUNX3 on FOXP3 protein levels, CD4+ T cells were preactivated with 2 µg/ml phytohemagglutinin (Sigma-Aldrich) in serum-free AIM-V medium in the presence of 1 nmol/liter IL-2 (Roche) for 12 h, and then transfected with the vector pEGFPN1 containing the RUNX1 or RUNX3 fragment using the Nucleofector system (Amaxa Biosystems) and the program T-23. |
T321 | 1151-1267 | Sentence | denotes | FOXP3 expression was evaluated by flow cytometry after 48 h of culture in AIM-V medium containing 1 nmol/liter IL-2. |
events-check-again
Id | Subject | Object | Predicate | Lexical cue | Speculation |
---|---|---|---|---|---|
T23184 | 112-117 | Protein | denotes | FOXP3 | |
T23185 | 127-137 | Protein | denotes | luciferase | |
T23186 | 189-194 | Protein | denotes | RUNX1 | |
T23187 | 196-201 | Protein | denotes | RUNX3 | |
T23188 | 259-262 | Protein | denotes | CD4 | |
T23189 | 457-467 | Protein | denotes | luciferase | |
T23190 | 502-512 | Protein | denotes | luciferase | |
T23191 | 681-691 | Protein | denotes | luciferase | |
T23192 | 767-773 | Regulation | denotes | effect | true |
T23193 | 767-773 | Regulation | denotes | effect | true |
T23194 | 777-791 | Gene_expression | denotes | overexpression | |
T23195 | 777-791 | Gene_expression | denotes | overexpression | |
T23196 | 795-800 | Protein | denotes | RUNX1 | |
T23197 | 804-809 | Protein | denotes | RUNX3 | |
T23198 | 813-818 | Protein | denotes | FOXP3 | |
T23199 | 835-838 | Protein | denotes | CD4 | |
T23200 | 879-897 | Protein | denotes | phytohemagglutinin | |
T23201 | 973-977 | Protein | denotes | IL-2 | |
T23202 | 1005-1016 | Gene_expression | denotes | transfected | |
T23203 | 1005-1016 | Gene_expression | denotes | transfected | |
T23204 | 1056-1061 | Protein | denotes | RUNX1 | |
T23205 | 1065-1070 | Protein | denotes | RUNX3 | |
T23206 | 1151-1156 | Protein | denotes | FOXP3 | |
T23207 | 1157-1167 | Gene_expression | denotes | expression | true |
T23208 | 1262-1266 | Protein | denotes | IL-2 | |
R17973 | T23194 | T23192 | causeOf | overexpression,effect | |
R17974 | T23195 | T23193 | causeOf | overexpression,effect | |
R17975 | T23196 | T23195 | themeOf | RUNX1,overexpression | |
R17976 | T23197 | T23194 | themeOf | RUNX3,overexpression | |
R17977 | T23198 | T23193 | themeOf | FOXP3,effect | |
R17978 | T23198 | T23192 | themeOf | FOXP3,effect | |
R17979 | T23204 | T23203 | themeOf | RUNX1,transfected | |
R17980 | T23205 | T23202 | themeOf | RUNX3,transfected | |
R17981 | T23206 | T23207 | themeOf | FOXP3,expression |
bionlp-st-ge-2016-reference-tees
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T23234 | 112-146 | Protein | denotes | FOXP3 promoter luciferase reporter |
T23235 | 189-194 | Protein | denotes | RUNX1 |
T23236 | 196-209 | Protein | denotes | RUNX3 pEGFPN1 |
T23237 | 457-467 | Protein | denotes | luciferase |
T23238 | 502-512 | Protein | denotes | luciferase |
T23239 | 681-691 | Protein | denotes | luciferase |
T23240 | 795-800 | Protein | denotes | RUNX1 |
T23241 | 804-809 | Protein | denotes | RUNX3 |
T23242 | 813-826 | Protein | denotes | FOXP3 protein |
T23243 | 835-839 | Protein | denotes | CD4+ |
T23244 | 879-897 | Protein | denotes | phytohemagglutinin |
T23245 | 899-912 | Protein | denotes | Sigma-Aldrich |
T23246 | 1056-1061 | Protein | denotes | RUNX1 |
T23247 | 1065-1079 | Protein | denotes | RUNX3 fragment |
T23248 | 777-791 | Gene_expression | denotes | overexpression |
T23249 | 777-791 | Gene_expression | denotes | overexpression |
T23250 | 777-791 | Gene_expression | denotes | overexpression |
T23251 | 1041-1051 | Gene_expression | denotes | containing |
T23252 | 1041-1051 | Gene_expression | denotes | containing |
T23253 | 767-773 | Regulation | denotes | effect |
T23254 | 767-773 | Regulation | denotes | effect |
T23255 | 767-773 | Regulation | denotes | effect |
T23256 | 1151-1156 | Protein | denotes | FOXP3 |
T23257 | 1157-1167 | Gene_expression | denotes | expression |
R17991 | T23240 | T23248 | themeOf | RUNX1,overexpression |
R17992 | T23241 | T23249 | themeOf | RUNX3,overexpression |
R17993 | T23242 | T23250 | themeOf | FOXP3 protein,overexpression |
R17994 | T23246 | T23251 | themeOf | RUNX1,containing |
R17995 | T23247 | T23252 | themeOf | RUNX3 fragment,containing |
R17996 | T23248 | T23253 | themeOf | overexpression,effect |
R17997 | T23249 | T23254 | themeOf | overexpression,effect |
R17998 | T23250 | T23255 | themeOf | overexpression,effect |
R17999 | T23256 | T23257 | themeOf | FOXP3,expression |
bionlp-st-ge-2016-reference
Id | Subject | Object | Predicate | Lexical cue | Speculation |
---|---|---|---|---|---|
T22644 | 112-117 | Protein | denotes | FOXP3 | |
T22645 | 127-137 | Protein | denotes | luciferase | |
T22646 | 189-194 | Protein | denotes | RUNX1 | |
T22647 | 196-201 | Protein | denotes | RUNX3 | |
T22648 | 259-262 | Protein | denotes | CD4 | |
T22649 | 457-467 | Protein | denotes | luciferase | |
T22650 | 502-512 | Protein | denotes | luciferase | |
T22651 | 681-691 | Protein | denotes | luciferase | |
T22652 | 767-773 | Regulation | denotes | effect | true |
T22653 | 767-773 | Regulation | denotes | effect | true |
T22654 | 777-791 | Gene_expression | denotes | overexpression | |
T22655 | 777-791 | Gene_expression | denotes | overexpression | |
T22656 | 795-800 | Protein | denotes | RUNX1 | |
T22657 | 804-809 | Protein | denotes | RUNX3 | |
T22658 | 813-818 | Protein | denotes | FOXP3 | |
T22659 | 835-838 | Protein | denotes | CD4 | |
T22660 | 879-897 | Protein | denotes | phytohemagglutinin | |
T22661 | 973-977 | Protein | denotes | IL-2 | |
T22662 | 1005-1016 | Gene_expression | denotes | transfected | |
T22663 | 1005-1016 | Gene_expression | denotes | transfected | |
T22664 | 1056-1061 | Protein | denotes | RUNX1 | |
T22665 | 1065-1070 | Protein | denotes | RUNX3 | |
T22666 | 1151-1156 | Protein | denotes | FOXP3 | |
T22667 | 1157-1167 | Gene_expression | denotes | expression | true |
T22668 | 1262-1266 | Protein | denotes | IL-2 | |
R17510 | T22654 | T22652 | causeOf | overexpression,effect | |
R17511 | T22655 | T22653 | causeOf | overexpression,effect | |
R17512 | T22656 | T22655 | themeOf | RUNX1,overexpression | |
R17513 | T22657 | T22654 | themeOf | RUNX3,overexpression | |
R17514 | T22658 | T22653 | themeOf | FOXP3,effect | |
R17515 | T22658 | T22652 | themeOf | FOXP3,effect | |
R17516 | T22664 | T22663 | themeOf | RUNX1,transfected | |
R17517 | T22665 | T22662 | themeOf | RUNX3,transfected | |
R17518 | T22666 | T22667 | themeOf | FOXP3,expression |
bionlp-st-ge-2016-uniprot
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T22890 | 74-77 | P26358 | denotes | AIM |
T22891 | 112-117 | Q9BZS1 | denotes | FOXP3 |
T22892 | 127-137 | P08659 | denotes | luciferase |
T22893 | 189-194 | Q01196 | denotes | RUNX1 |
T22894 | 196-201 | Q13761 | denotes | RUNX3 |
T22895 | 259-262 | P01730 | denotes | CD4 |
T22896 | 457-467 | P08659 | denotes | luciferase |
T22897 | 502-512 | P08659 | denotes | luciferase |
T22898 | 681-691 | P08659 | denotes | luciferase |
T22899 | 795-800 | Q01196 | denotes | RUNX1 |
T22900 | 804-809 | Q13761 | denotes | RUNX3 |
T22901 | 813-818 | Q9BZS1 | denotes | FOXP3 |
T22902 | 835-838 | P01730 | denotes | CD4 |
T22903 | 928-931 | P26358 | denotes | AIM |
T22904 | 973-977 | P60568 | denotes | IL-2 |
T22905 | 1056-1061 | Q01196 | denotes | RUNX1 |
T22906 | 1065-1070 | Q13761 | denotes | RUNX3 |
T22907 | 1151-1156 | Q9BZS1 | denotes | FOXP3 |
T22908 | 1225-1228 | P26358 | denotes | AIM |
T22909 | 1262-1266 | P60568 | denotes | IL-2 |
test2
Id | Subject | Object | Predicate | Lexical cue | Speculation |
---|---|---|---|---|---|
T22620 | 112-117 | Protein | denotes | FOXP3 | true |
T22621 | 127-137 | Protein | denotes | luciferase | |
T22622 | 189-194 | Protein | denotes | RUNX1 | true |
T22623 | 196-201 | Protein | denotes | RUNX3 | |
T22624 | 259-262 | Protein | denotes | CD4 | |
T22625 | 457-467 | Protein | denotes | luciferase | |
T22626 | 502-512 | Protein | denotes | luciferase | true |
T22627 | 681-691 | Protein | denotes | luciferase | |
T22628 | 767-773 | Regulation | denotes | effect | |
T22629 | 777-791 | Positive_regulation | denotes | overexpression | |
T22630 | 795-800 | Protein | denotes | RUNX1 | |
T22631 | 804-809 | Protein | denotes | RUNX3 | |
T22632 | 813-818 | Protein | denotes | FOXP3 | |
T22633 | 835-838 | Protein | denotes | CD4 | |
T22634 | 879-897 | Protein | denotes | phytohemagglutinin | |
T22635 | 973-977 | Protein | denotes | IL-2 | |
T22636 | 1056-1061 | Protein | denotes | RUNX1 | |
T22637 | 1065-1070 | Protein | denotes | RUNX3 | |
T22638 | 1151-1156 | Protein | denotes | FOXP3 | |
T22639 | 1157-1167 | Gene_expression | denotes | expression | |
T22640 | 1262-1266 | Protein | denotes | IL-2 | |
R17505 | T22629 | T22628 | causeOf | overexpression,effect | |
R17506 | T22630 | T22629 | themeOf | RUNX1,overexpression | |
R17507 | T22631 | T22629 | themeOf | RUNX3,overexpression | |
R17508 | T22632 | T22629 | themeOf | FOXP3,overexpression | |
R17509 | T22638 | T22639 | themeOf | FOXP3,expression |