PMC:2800179 / 8662-9456 JSON TXT

Annnotations TAB JSON ListView MergeView

GO-CC

{"project":"GO-CC","denotations":[{"id":"T3977","span":{"begin":0,"end":4},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T3978","span":{"begin":557,"end":561},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T3979","span":{"begin":715,"end":723},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T3980","span":{"begin":715,"end":723},"obj":"http://purl.obolibrary.org/obo/GO_0042571"}],"text":"Cell Culture\nAll tissue culture reagents were from Invitrogen (Cergy Pontoise, France). THP-1 human monocytic cells were maintained in RPMI-1640 supplemented with 10% FCS, 2 mM L -glutamine, 50 U/ml penicillin and 50 mg/ml streptomycin at 37°C in a 5% CO2 incubator. Human peripheral blood mononuclear cells were obtained from heparinized blood by Ficoll-Hypaque density gradient. Monocytes were then isolated by adherence to culture flasks as described [28]. For cell aggregation, monocytes were cultured in the presence or absence of C10 or C40 for 72 h. Cell colonies were monitored under an inverted phase contrast microscope coupled through a video camera to a computer. In some wells, neutralizing monoclonal antibody to ICAM-1 (2.5 µg/ml) (Tebu, Le Perray en Yvelines, France) was added."}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T3976","span":{"begin":715,"end":723},"obj":"http://purl.obolibrary.org/obo/GO_0003823"}],"text":"Cell Culture\nAll tissue culture reagents were from Invitrogen (Cergy Pontoise, France). THP-1 human monocytic cells were maintained in RPMI-1640 supplemented with 10% FCS, 2 mM L -glutamine, 50 U/ml penicillin and 50 mg/ml streptomycin at 37°C in a 5% CO2 incubator. Human peripheral blood mononuclear cells were obtained from heparinized blood by Ficoll-Hypaque density gradient. Monocytes were then isolated by adherence to culture flasks as described [28]. For cell aggregation, monocytes were cultured in the presence or absence of C10 or C40 for 72 h. Cell colonies were monitored under an inverted phase contrast microscope coupled through a video camera to a computer. In some wells, neutralizing monoclonal antibody to ICAM-1 (2.5 µg/ml) (Tebu, Le Perray en Yvelines, France) was added."}

GO-BP

{"project":"GO-BP","denotations":[{"id":"T3974","span":{"begin":464,"end":480},"obj":"http://purl.obolibrary.org/obo/GO_0098743"},{"id":"T3975","span":{"begin":469,"end":491},"obj":"http://purl.obolibrary.org/obo/GO_0070487"}],"text":"Cell Culture\nAll tissue culture reagents were from Invitrogen (Cergy Pontoise, France). THP-1 human monocytic cells were maintained in RPMI-1640 supplemented with 10% FCS, 2 mM L -glutamine, 50 U/ml penicillin and 50 mg/ml streptomycin at 37°C in a 5% CO2 incubator. Human peripheral blood mononuclear cells were obtained from heparinized blood by Ficoll-Hypaque density gradient. Monocytes were then isolated by adherence to culture flasks as described [28]. For cell aggregation, monocytes were cultured in the presence or absence of C10 or C40 for 72 h. Cell colonies were monitored under an inverted phase contrast microscope coupled through a video camera to a computer. In some wells, neutralizing monoclonal antibody to ICAM-1 (2.5 µg/ml) (Tebu, Le Perray en Yvelines, France) was added."}

2_test

{"project":"2_test","denotations":[{"id":"20072622-8482919-93233366","span":{"begin":455,"end":457},"obj":"8482919"}],"text":"Cell Culture\nAll tissue culture reagents were from Invitrogen (Cergy Pontoise, France). THP-1 human monocytic cells were maintained in RPMI-1640 supplemented with 10% FCS, 2 mM L -glutamine, 50 U/ml penicillin and 50 mg/ml streptomycin at 37°C in a 5% CO2 incubator. Human peripheral blood mononuclear cells were obtained from heparinized blood by Ficoll-Hypaque density gradient. Monocytes were then isolated by adherence to culture flasks as described [28]. For cell aggregation, monocytes were cultured in the presence or absence of C10 or C40 for 72 h. Cell colonies were monitored under an inverted phase contrast microscope coupled through a video camera to a computer. In some wells, neutralizing monoclonal antibody to ICAM-1 (2.5 µg/ml) (Tebu, Le Perray en Yvelines, France) was added."}

pmc-enju-pas

bionlp-st-ge-2016-spacy-parsed

UBERON-AE

{"project":"UBERON-AE","denotations":[{"id":"T3665","span":{"begin":17,"end":23},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T3666","span":{"begin":284,"end":289},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"T3667","span":{"begin":339,"end":344},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"}],"text":"Cell Culture\nAll tissue culture reagents were from Invitrogen (Cergy Pontoise, France). THP-1 human monocytic cells were maintained in RPMI-1640 supplemented with 10% FCS, 2 mM L -glutamine, 50 U/ml penicillin and 50 mg/ml streptomycin at 37°C in a 5% CO2 incubator. Human peripheral blood mononuclear cells were obtained from heparinized blood by Ficoll-Hypaque density gradient. Monocytes were then isolated by adherence to culture flasks as described [28]. For cell aggregation, monocytes were cultured in the presence or absence of C10 or C40 for 72 h. Cell colonies were monitored under an inverted phase contrast microscope coupled through a video camera to a computer. In some wells, neutralizing monoclonal antibody to ICAM-1 (2.5 µg/ml) (Tebu, Le Perray en Yvelines, France) was added."}

sentences

{"project":"sentences","denotations":[{"id":"T3668","span":{"begin":0,"end":12},"obj":"Sentence"},{"id":"T3669","span":{"begin":13,"end":87},"obj":"Sentence"},{"id":"T3670","span":{"begin":88,"end":266},"obj":"Sentence"},{"id":"T3671","span":{"begin":267,"end":380},"obj":"Sentence"},{"id":"T3672","span":{"begin":381,"end":459},"obj":"Sentence"},{"id":"T3673","span":{"begin":460,"end":556},"obj":"Sentence"},{"id":"T3674","span":{"begin":557,"end":675},"obj":"Sentence"},{"id":"T3675","span":{"begin":676,"end":794},"obj":"Sentence"},{"id":"T75","span":{"begin":0,"end":12},"obj":"Sentence"},{"id":"T76","span":{"begin":13,"end":87},"obj":"Sentence"},{"id":"T77","span":{"begin":88,"end":266},"obj":"Sentence"},{"id":"T78","span":{"begin":267,"end":380},"obj":"Sentence"},{"id":"T79","span":{"begin":381,"end":459},"obj":"Sentence"},{"id":"T80","span":{"begin":460,"end":556},"obj":"Sentence"},{"id":"T81","span":{"begin":557,"end":675},"obj":"Sentence"},{"id":"T82","span":{"begin":676,"end":794},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Cell Culture\nAll tissue culture reagents were from Invitrogen (Cergy Pontoise, France). THP-1 human monocytic cells were maintained in RPMI-1640 supplemented with 10% FCS, 2 mM L -glutamine, 50 U/ml penicillin and 50 mg/ml streptomycin at 37°C in a 5% CO2 incubator. Human peripheral blood mononuclear cells were obtained from heparinized blood by Ficoll-Hypaque density gradient. Monocytes were then isolated by adherence to culture flasks as described [28]. For cell aggregation, monocytes were cultured in the presence or absence of C10 or C40 for 72 h. Cell colonies were monitored under an inverted phase contrast microscope coupled through a video camera to a computer. In some wells, neutralizing monoclonal antibody to ICAM-1 (2.5 µg/ml) (Tebu, Le Perray en Yvelines, France) was added."}

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T3815","span":{"begin":727,"end":733},"obj":"P05362"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Cell Culture\nAll tissue culture reagents were from Invitrogen (Cergy Pontoise, France). THP-1 human monocytic cells were maintained in RPMI-1640 supplemented with 10% FCS, 2 mM L -glutamine, 50 U/ml penicillin and 50 mg/ml streptomycin at 37°C in a 5% CO2 incubator. Human peripheral blood mononuclear cells were obtained from heparinized blood by Ficoll-Hypaque density gradient. Monocytes were then isolated by adherence to culture flasks as described [28]. For cell aggregation, monocytes were cultured in the presence or absence of C10 or C40 for 72 h. Cell colonies were monitored under an inverted phase contrast microscope coupled through a video camera to a computer. In some wells, neutralizing monoclonal antibody to ICAM-1 (2.5 µg/ml) (Tebu, Le Perray en Yvelines, France) was added."}

PMC:2800179 / 8662-9456 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:2800179 / 8662-9456 JSON TXT