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PMC:2800179 / 4622-15756 JSONTXT

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GO-CC

Id Subject Object Predicate Lexical cue
T2807 1931-1935 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T3977 4040-4044 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T3978 4597-4601 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T3979 4755-4763 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T3980 4755-4763 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T4227 4836-4840 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T4228 5137-5141 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T4229 5283-5288 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4547 5532-5536 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T4548 5609-5614 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4549 5746-5751 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4550 5868-5873 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4551 6116-6121 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4552 6333-6338 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4553 5532-5544 http://purl.obolibrary.org/obo/GO_0009986 denotes Cell Surface
T4554 5902-5912 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T4555 6002-6010 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T4556 5902-5912 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T4557 6002-6010 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T4715 6382-6387 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4716 6617-6621 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T5447 8125-8130 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6420 8406-8411 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6421 9423-9428 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6422 8963-8971 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T6423 9788-9796 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T6424 9836-9845 http://purl.obolibrary.org/obo/GO_0016020 denotes Membranes
T6425 9932-9941 http://purl.obolibrary.org/obo/GO_0016020 denotes Membranes
T6426 10169-10178 http://purl.obolibrary.org/obo/GO_0016020 denotes Membranes
T6427 10702-10711 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T6428 9026-9034 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6429 9127-9135 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6430 9990-9998 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6431 10048-10056 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6432 10471-10479 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6433 9026-9034 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T6434 9127-9135 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T6435 9990-9998 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T6436 10048-10056 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T6437 10471-10479 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody

GO-MF

Id Subject Object Predicate Lexical cue
T3641 3769-3772 http://purl.obolibrary.org/obo/GO_0004601 denotes MPO
T3642 3828-3831 http://purl.obolibrary.org/obo/GO_0004601 denotes MPO
T3643 3953-3956 http://purl.obolibrary.org/obo/GO_0004601 denotes MPO
T3976 4755-4763 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T4226 5443-5445 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T4544 5831-5838 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T4545 5902-5912 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T4546 6002-6010 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T5200 6686-6688 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T5201 6867-6880 http://purl.obolibrary.org/obo/GO_0003899 denotes transcriptase
T5202 6867-6880 http://purl.obolibrary.org/obo/GO_0003968 denotes transcriptase
T5203 6867-6880 http://purl.obolibrary.org/obo/GO_0034062 denotes transcriptase
T5442 8277-8296 http://purl.obolibrary.org/obo/GO_0045289 denotes Luciferase activity
T5443 8277-8296 http://purl.obolibrary.org/obo/GO_0047077 denotes Luciferase activity
T5444 8277-8296 http://purl.obolibrary.org/obo/GO_0047712 denotes Luciferase activity
T5445 8277-8296 http://purl.obolibrary.org/obo/GO_0050248 denotes Luciferase activity
T5446 8277-8296 http://purl.obolibrary.org/obo/GO_0050397 denotes Luciferase activity
T6413 9026-9034 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T6414 9127-9135 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T6415 9990-9998 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T6416 10048-10056 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T6417 10471-10479 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T6418 9062-9064 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T6419 10742-10745 http://purl.obolibrary.org/obo/GO_0004707 denotes ERK

GO-BP

Id Subject Object Predicate Lexical cue
T2804 38-46 http://purl.obolibrary.org/obo/GO_0009056 denotes Degraded
T2805 79-87 http://purl.obolibrary.org/obo/GO_0009056 denotes degraded
T2806 1548-1556 http://purl.obolibrary.org/obo/GO_0051923 denotes sulphate
T3085 2184-2192 http://purl.obolibrary.org/obo/GO_0009056 denotes Degraded
T3086 2236-2244 http://purl.obolibrary.org/obo/GO_0007631 denotes drinking
T3087 2602-2610 http://purl.obolibrary.org/obo/GO_0007631 denotes drinking
T3634 3393-3405 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T3635 3498-3510 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T3636 3620-3629 http://purl.obolibrary.org/obo/GO_0051923 denotes sulphated
T3637 3769-3772 http://purl.obolibrary.org/obo/GO_0004601 denotes MPO
T3638 3828-3831 http://purl.obolibrary.org/obo/GO_0004601 denotes MPO
T3639 3953-3956 http://purl.obolibrary.org/obo/GO_0004601 denotes MPO
T3640 3985-3996 http://purl.obolibrary.org/obo/GO_0009056 denotes degradation
T3974 4504-4520 http://purl.obolibrary.org/obo/GO_0098743 denotes cell aggregation
T3975 4509-4531 http://purl.obolibrary.org/obo/GO_0070487 denotes aggregation, monocytes
T4223 4836-4846 http://purl.obolibrary.org/obo/GO_0007049 denotes Cell Cycle
T4224 4883-4889 http://purl.obolibrary.org/obo/GO_0040007 denotes growth
T4225 5443-5445 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T4714 6617-6629 http://purl.obolibrary.org/obo/GO_0001906 denotes cell killing
T5196 6686-6688 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T5197 6867-6880 http://purl.obolibrary.org/obo/GO_0003899 denotes transcriptase
T5198 6867-6880 http://purl.obolibrary.org/obo/GO_0003968 denotes transcriptase
T5199 6867-6880 http://purl.obolibrary.org/obo/GO_0034062 denotes transcriptase
T5436 7829-7842 http://purl.obolibrary.org/obo/GO_0006351 denotes Transcription
T5437 8277-8296 http://purl.obolibrary.org/obo/GO_0045289 denotes Luciferase activity
T5438 8277-8296 http://purl.obolibrary.org/obo/GO_0047077 denotes Luciferase activity
T5439 8277-8296 http://purl.obolibrary.org/obo/GO_0047712 denotes Luciferase activity
T5440 8277-8296 http://purl.obolibrary.org/obo/GO_0050248 denotes Luciferase activity
T5441 8277-8296 http://purl.obolibrary.org/obo/GO_0050397 denotes Luciferase activity
T6409 8552-8557 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T6410 8712-8720 http://purl.obolibrary.org/obo/GO_0007349 denotes cellular
T6411 9062-9064 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T6412 10742-10745 http://purl.obolibrary.org/obo/GO_0004707 denotes ERK

2_test

Id Subject Object Predicate Lexical cue
20072622-4212170-93233361 2655-2657 4212170 denotes 23
20072622-1688816-93233362 3012-3014 1688816 denotes 24
20072622-9824605-93233363 3550-3552 9824605 denotes 25
20072622-10940278-93233364 3556-3558 10940278 denotes 26
20072622-6092199-93233365 3810-3812 6092199 denotes 27
20072622-8482919-93233366 4495-4497 8482919 denotes 28
20072622-3782828-93233367 6637-6639 3782828 denotes 29
20072622-8625304-93233368 8185-8187 8625304 denotes 30
20072622-19342628-93233369 10760-10762 19342628 denotes 32

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T2063 23-34 NN denotes Preparation
T2064 35-37 IN denotes of
T2065 38-46 NNP denotes Degraded
T2066 47-58 NNP denotes Carrageenan
T2067 59-62 CD denotes Two
T2068 63-75 NN denotes preparations
T2069 76-78 IN denotes of
T2070 79-87 VB denotes degraded
T2071 88-99 NN denotes carrageenan
T2072 100-104 IN denotes with
T2073 105-108 JJ denotes low
T2074 108-109 -COMMA- denotes ,
T2075 110-111 -LRB- denotes (
T2076 111-114 CD denotes ∼10
T2077 115-118 NN denotes kDa
T2078 118-119 -COLON- denotes ;
T2079 120-123 NN denotes C10
T2080 123-124 -RRB- denotes )
T2081 124-125 -COMMA- denotes ,
T2082 126-129 CC denotes and
T2083 130-136 NN denotes medium
T2084 136-137 -COMMA- denotes ,
T2085 138-139 -LRB- denotes (
T2086 139-142 CD denotes ∼40
T2087 143-146 NN denotes kDa
T2088 146-147 -COLON- denotes ;
T2089 148-151 NN denotes C40
T2090 151-152 -RRB- denotes )
T2091 153-162 JJ denotes molecular
T2092 163-169 NN denotes weight
T2093 170-174 VB denotes were
T2094 175-183 VB denotes prepared
T2095 184-188 IN denotes from
T2096 189-195 JJ denotes native
T2097 196-212 NN denotes iota-carrageenan
T2098 213-222 VB denotes extracted
T2099 223-227 IN denotes from
T2100 228-235 NNP denotes Euchema
T2101 236-244 NN denotes spinosum
T2102 245-246 -LRB- denotes (
T2103 246-256 RB denotes generously
T2104 257-265 VB denotes provided
T2105 266-268 IN denotes by
T2106 269-275 NNP denotes Sanofi
T2107 276-286 NNP denotes Biosystems
T2108 287-295 NNP denotes Industry
T2109 295-296 -COMMA- denotes ,
T2110 297-317 NNP denotes Boulogne-Billancourt
T2111 317-318 -COMMA- denotes ,
T2112 319-325 NNP denotes France
T2113 325-326 -RRB- denotes )
T2114 328-334 JJ denotes Native
T2115 335-346 NN denotes carrageenan
T2116 347-350 VB denotes was
T2117 351-360 VB denotes dissolved
T2118 361-363 IN denotes in
T2119 364-373 JJ denotes distilled
T2120 374-379 NN denotes water
T2121 380-381 -LRB- denotes (
T2122 381-382 CD denotes 5
T2123 382-383 NN denotes %
T2124 384-387 NN denotes w/v
T2125 387-388 -RRB- denotes )
T2126 389-394 IN denotes under
T2127 395-403 JJ denotes vigorous
T2128 404-412 VB denotes stirring
T2129 413-416 CC denotes and
T2130 417-423 VB denotes heated
T2131 424-426 TO denotes to
T2132 427-432 NNP denotes 60°C.
T2133 433-437 NNP denotes Then
T2134 437-438 -COMMA- denotes ,
T2135 439-442 DT denotes the
T2136 443-454 NN denotes carrageenan
T2137 455-463 NN denotes solution
T2138 464-467 VB denotes was
T2139 468-477 VB denotes submitted
T2140 478-480 TO denotes to
T2141 481-484 CD denotes two
T2142 485-494 JJ denotes different
T2143 495-505 NN denotes treatments
T2144 506-508 TO denotes to
T2145 509-515 VB denotes obtain
T2146 516-520 CC denotes both
T2147 521-524 JJ denotes low
T2148 525-528 CC denotes and
T2149 529-535 JJ denotes medium
T2150 536-545 JJ denotes molecular
T2151 546-552 NN denotes weight
T2152 553-562 NN denotes fractions
T2153 564-571 RB denotes Briefly
T2154 571-572 -COMMA- denotes ,
T2155 573-576 IN denotes for
T2156 577-580 DT denotes the
T2157 581-584 JJ denotes low
T2158 585-594 JJ denotes molecular
T2159 595-601 NN denotes weight
T2160 602-610 NN denotes fraction
T2161 610-611 -COMMA- denotes ,
T2162 612-623 NN denotes carrageenan
T2163 624-632 NN denotes solution
T2164 633-636 VB denotes was
T2165 637-647 VB denotes hydrolyzed
T2166 648-652 IN denotes with
T2167 653-656 CD denotes 0.3
T2168 656-657 NN denotes %
T2169 658-659 -LRB- denotes (
T2170 659-662 NN denotes v/v
T2171 662-663 -RRB- denotes )
T2172 664-676 VB denotes concentrated
T2173 677-686 JJ denotes sulphuric
T2174 687-691 NN denotes acid
T2175 692-695 IN denotes for
T2176 696-698 CD denotes 15
T2177 699-702 NN denotes min
T2178 703-705 IN denotes at
T2179 706-711 NNP denotes 80°C.
T2180 712-717 NNP denotes After
T2181 718-732 NN denotes neutralization
T2182 733-737 IN denotes with
T2183 738-742 NN denotes NaOH
T2184 743-745 NN denotes 4N
T2185 745-746 -COMMA- denotes ,
T2186 747-750 DT denotes the
T2187 751-759 NN denotes solution
T2188 760-763 VB denotes was
T2189 764-769 NN denotes ultra
T2190 770-778 VB denotes filtered
T2191 779-786 IN denotes through
T2192 787-788 DT denotes a
T2193 789-795 JJ denotes hollow
T2194 796-801 NN denotes fibre
T2195 802-811 NN denotes cartridge
T2196 812-816 IN denotes with
T2197 817-819 NN denotes MW
T2198 820-827 JJ denotes cut-off
T2199 828-829 CD denotes 5
T2200 830-833 NN denotes kDa
T2201 833-834 -COMMA- denotes ,
T2202 835-836 -LRB- denotes (
T2203 836-842 NNP denotes Amicon
T2204 843-846 NNP denotes Inc
T2205 846-847 -COMMA- denotes ,
T2206 848-855 NNP denotes Beverly
T2207 855-856 -COMMA- denotes ,
T2208 857-860 NNP denotes USA
T2209 860-861 -RRB- denotes )
T2210 863-866 IN denotes For
T2211 867-870 DT denotes the
T2212 871-877 NN denotes medium
T2213 878-887 JJ denotes molecular
T2214 888-894 NN denotes weight
T2215 895-903 NN denotes fraction
T2216 903-904 -COMMA- denotes ,
T2217 905-908 DT denotes the
T2218 909-920 NN denotes carrageenan
T2219 921-929 NN denotes solution
T2220 930-933 VB denotes was
T2221 934-944 VB denotes hydrolyzed
T2222 945-949 IN denotes with
T2223 950-953 CD denotes 0.3
T2224 953-954 NN denotes %
T2225 955-956 -LRB- denotes (
T2226 956-959 NN denotes v/v
T2227 959-960 -RRB- denotes )
T2228 961-973 VB denotes concentrated
T2229 974-983 JJ denotes sulphuric
T2230 984-988 NN denotes acid
T2231 989-992 IN denotes for
T2232 993-995 CD denotes 30
T2233 996-999 NN denotes min
T2234 1000-1002 IN denotes at
T2235 1003-1008 NNP denotes 60°C.
T2236 1009-1014 IN denotes After
T2237 1015-1029 NN denotes neutralization
T2238 1029-1030 -COMMA- denotes ,
T2239 1031-1034 DT denotes the
T2240 1035-1046 NN denotes supernatant
T2241 1047-1050 VB denotes was
T2242 1051-1056 AFX denotes ultra
T2243 1057-1065 VB denotes filtered
T2244 1066-1067 -LRB- denotes (
T2245 1067-1069 NN denotes MW
T2246 1070-1077 JJ denotes cut-off
T2247 1078-1081 CD denotes 100
T2248 1082-1085 NN denotes kDa
T2249 1085-1086 -RRB- denotes )
T2250 1088-1091 DT denotes The
T2251 1092-1100 NN denotes filtrate
T2252 1101-1104 VB denotes was
T2253 1105-1114 VB denotes submitted
T2254 1115-1117 TO denotes to
T2255 1118-1119 DT denotes a
T2256 1120-1126 JJ denotes second
T2257 1127-1132 NN denotes ultra
T2258 1133-1143 NN denotes filtration
T2259 1144-1145 -LRB- denotes (
T2260 1145-1147 NN denotes MW
T2261 1148-1155 NN denotes cut-off
T2262 1156-1157 CD denotes 5
T2263 1158-1161 NN denotes kDa
T2264 1161-1162 -RRB- denotes )
T2265 1164-1168 DT denotes Both
T2266 1169-1181 NN denotes preparations
T2267 1182-1184 IN denotes of
T2268 1185-1189 NN denotes dCGN
T2269 1190-1194 VB denotes were
T2270 1195-1207 VB denotes precipitated
T2271 1208-1212 IN denotes with
T2272 1213-1214 CD denotes 4
T2273 1215-1222 NN denotes volumes
T2274 1223-1225 IN denotes of
T2275 1226-1228 CD denotes 95
T2276 1228-1229 NN denotes %
T2277 1230-1237 NN denotes ethanol
T2278 1237-1238 -COMMA- denotes ,
T2279 1239-1244 VB denotes dried
T2280 1245-1247 IN denotes at
T2281 1248-1252 NN denotes room
T2282 1253-1264 NN denotes temperature
T2283 1265-1268 CC denotes and
T2284 1269-1275 NN denotes ground
T2285 1276-1278 TO denotes to
T2286 1279-1284 JJ denotes small
T2287 1285-1294 NN denotes particles
T2288 1295-1296 -LRB- denotes (
T2289 1296-1297 CD denotes 1
T2290 1298-1300 NN denotes mm
T2291 1301-1303 IN denotes in
T2292 1304-1312 NN denotes diameter
T2293 1312-1313 -RRB- denotes )
T2294 1315-1320 VB denotes Using
T2295 1321-1335 NN denotes gel-permeation
T2296 1336-1350 NN denotes chromatography
T2297 1351-1353 IN denotes in
T2298 1354-1365 NN denotes combination
T2299 1366-1370 IN denotes with
T2300 1371-1376 JJ denotes light
T2301 1377-1387 NN denotes scattering
T2302 1388-1400 NN denotes measurements
T2303 1401-1402 -LRB- denotes (
T2304 1402-1405 VB denotes see
T2305 1406-1412 NNP denotes Viebke
T2306 1413-1415 FW denotes et
T2307 1416-1419 FW denotes al.
T2308 1420-1421 -LRB- denotes [
T2309 1421-1423 CD denotes 21
T2310 1423-1424 -RRB- denotes ]
T2311 1424-1425 -RRB- denotes )
T2312 1425-1426 -COMMA- denotes ,
T2313 1427-1429 PRP denotes it
T2314 1430-1433 VB denotes was
T2315 1434-1443 VB denotes confirmed
T2316 1444-1448 IN denotes that
T2317 1449-1452 DT denotes the
T2318 1453-1456 JJ denotes low
T2319 1457-1465 NN denotes fraction
T2320 1466-1469 VB denotes had
T2321 1470-1472 DT denotes an
T2322 1473-1480 JJ denotes average
T2323 1481-1490 JJ denotes molecular
T2324 1491-1497 NN denotes weight
T2325 1498-1500 IN denotes of
T2326 1501-1503 CD denotes 10
T2327 1504-1507 NN denotes kDa
T2328 1507-1508 -COMMA- denotes ,
T2329 1509-1512 CC denotes and
T2330 1513-1516 DT denotes the
T2331 1517-1523 NN denotes medium
T2332 1524-1532 NN denotes fraction
T2333 1533-1535 IN denotes of
T2334 1536-1538 CD denotes 40
T2335 1539-1542 NN denotes kDa
T2336 1544-1547 DT denotes The
T2337 1548-1556 NN denotes sulphate
T2338 1557-1564 NN denotes content
T2339 1565-1567 IN denotes of
T2340 1568-1583 NN denotes polysaccharides
T2341 1584-1586 IN denotes in
T2342 1587-1591 DT denotes both
T2343 1592-1601 NN denotes fractions
T2344 1602-1605 VB denotes was
T2345 1606-1614 VB denotes measured
T2346 1615-1624 VB denotes following
T2347 1625-1628 DT denotes the
T2348 1629-1635 NN denotes method
T2349 1636-1638 IN denotes of
T2350 1639-1647 NNP denotes Quemener
T2351 1648-1650 FW denotes et
T2352 1651-1654 FW denotes al.
T2353 1655-1656 -LRB- denotes [
T2354 1656-1658 CD denotes 22
T2355 1658-1659 -RRB- denotes ]
T2356 1661-1668 RB denotes Finally
T2357 1668-1669 -COMMA- denotes ,
T2358 1670-1673 DT denotes the
T2359 1674-1681 NN denotes absence
T2360 1682-1684 IN denotes of
T2361 1685-1699 NN denotes polysaccharide
T2362 1700-1709 NN denotes structure
T2363 1710-1723 NN denotes modifications
T2364 1724-1726 IN denotes in
T2365 1727-1730 DT denotes the
T2366 1731-1734 CD denotes two
T2367 1735-1744 NN denotes fractions
T2368 1745-1748 VB denotes was
T2369 1749-1758 VB denotes confirmed
T2370 1759-1764 VB denotes using
T2371 1765-1771 NN denotes 2H-NMR
T2372 1772-1784 NN denotes spectroscopy
T2373 1786-1789 DT denotes The
T2374 1790-1797 NN denotes absence
T2375 1798-1800 IN denotes of
T2376 1801-1804 NN denotes LPS
T2377 1805-1818 NN denotes contamination
T2378 1819-1821 IN denotes in
T2379 1822-1825 DT denotes the
T2380 1826-1829 CD denotes two
T2381 1830-1839 NN denotes fractions
T2382 1840-1843 VB denotes was
T2383 1844-1853 VB denotes confirmed
T2384 1854-1859 VB denotes using
T2385 1860-1863 DT denotes the
T2386 1864-1873 NN denotes e-Toxate®
T2387 1874-1877 NN denotes kit
T2388 1878-1879 -LRB- denotes (
T2389 1879-1884 NNP denotes Sigma
T2390 1884-1885 -COMMA- denotes ,
T2391 1886-1888 NNP denotes St
T2392 1889-1896 NNP denotes Quentin
T2393 1897-1906 NNP denotes Fallavier
T2394 1906-1907 -COMMA- denotes ,
T2395 1908-1914 NNP denotes France
T2396 1914-1915 -RRB- denotes )
T2397 1917-1923 IN denotes Before
T2398 1924-1927 NN denotes use
T2399 1928-1930 IN denotes in
T2400 1931-1935 NN denotes cell
T2401 1936-1943 NN denotes culture
T2402 1943-1944 -COMMA- denotes ,
T2403 1945-1948 DT denotes the
T2404 1949-1952 CD denotes two
T2405 1953-1962 NN denotes fractions
T2406 1963-1967 VB denotes were
T2407 1968-1977 VB denotes dissolved
T2408 1978-1980 IN denotes in
T2409 1981-1989 JJ denotes complete
T2410 1990-1996 NN denotes medium
T2411 1997-2003 IN denotes during
T2412 2004-2006 CD denotes 30
T2413 2007-2010 NN denotes min
T2414 2011-2013 IN denotes at
T2415 2014-2018 NN denotes 56°C
T2818 2028-2029 -COMMA- denotes ,
T2819 2030-2039 NNP denotes Chemicals
T2820 2040-2043 CC denotes and
T2821 2044-2048 NNP denotes Diet
T2822 2049-2053 JJ denotes Male
T2823 2054-2060 NNP denotes Wistar
T2824 2061-2065 NN denotes rats
T2825 2066-2067 -LRB- denotes (
T2826 2067-2070 CD denotes 150
T2827 2071-2072 NN denotes g
T2828 2073-2080 JJ denotes average
T2829 2081-2087 NN denotes weight
T2830 2087-2088 -RRB- denotes )
T2831 2089-2093 VB denotes were
T2832 2094-2100 VB denotes housed
T2833 2101-2106 IN denotes under
T2834 2107-2115 JJ denotes standard
T2835 2116-2126 NN denotes conditions
T2836 2127-2130 CC denotes and
T2837 2131-2134 VB denotes fed
T2838 2135-2137 NN denotes ad
T2839 2138-2145 NN denotes libitum
T2840 2146-2150 IN denotes with
T2841 2151-2159 JJ denotes standard
T2842 2160-2166 NN denotes rodent
T2843 2167-2177 NN denotes laboratory
T2844 2178-2182 NN denotes chow
T2845 2184-2192 VB denotes Degraded
T2846 2193-2210 NN denotes iota-carrageenans
T2847 2211-2215 VB denotes were
T2848 2216-2228 VB denotes administered
T2849 2229-2231 IN denotes in
T2850 2232-2235 DT denotes the
T2851 2236-2244 NN denotes drinking
T2852 2245-2250 NN denotes water
T2853 2251-2252 -LRB- denotes (
T2854 2252-2253 CD denotes 5
T2855 2253-2254 NN denotes %
T2856 2255-2258 NN denotes w/v
T2857 2258-2259 -RRB- denotes )
T2858 2260-2263 IN denotes for
T2859 2264-2266 CD denotes 55
T2860 2267-2271 NN denotes days
T2861 2272-2274 TO denotes to
T2862 2275-2276 CD denotes 2
T2863 2277-2283 NN denotes groups
T2864 2284-2286 IN denotes of
T2865 2287-2290 CD denotes six
T2866 2291-2298 NN denotes animals
T2867 2299-2303 DT denotes each
T2868 2305-2308 DT denotes The
T2869 2309-2314 JJ denotes first
T2870 2315-2320 NN denotes group
T2871 2321-2329 VB denotes received
T2872 2330-2333 DT denotes the
T2873 2334-2337 JJ denotes low
T2874 2338-2347 JJ denotes molecular
T2875 2348-2354 NN denotes weight
T2876 2355-2366 NN denotes carrageenan
T2877 2367-2368 -LRB- denotes (
T2878 2368-2370 CD denotes 10
T2879 2371-2374 NN denotes kDa
T2880 2375-2379 NN denotes dCGN
T2881 2379-2380 -RRB- denotes )
T2882 2381-2384 CC denotes and
T2883 2385-2388 DT denotes the
T2884 2389-2395 NN denotes second
T2885 2396-2404 VB denotes received
T2886 2405-2408 DT denotes the
T2887 2409-2415 NN denotes medium
T2888 2416-2425 JJ denotes molecular
T2889 2426-2432 NN denotes weight
T2890 2433-2444 NN denotes carrageenan
T2891 2445-2446 -LRB- denotes (
T2892 2446-2448 CD denotes 40
T2893 2449-2452 NN denotes kDa
T2894 2453-2457 NN denotes dCGN
T2895 2457-2458 -RRB- denotes )
T2896 2460-2462 DT denotes An
T2897 2463-2473 JJ denotes additional
T2898 2474-2479 NN denotes group
T2899 2480-2482 IN denotes of
T2900 2483-2487 CD denotes four
T2901 2488-2492 NN denotes rats
T2902 2493-2497 VB denotes were
T2903 2498-2508 VB denotes maintained
T2904 2509-2511 IN denotes on
T2905 2512-2519 JJ denotes regular
T2906 2520-2523 NN denotes tap
T2907 2524-2529 NN denotes water
T2908 2530-2531 -LRB- denotes (
T2909 2531-2538 NN denotes control
T2910 2539-2544 NN denotes group
T2911 2544-2545 -RRB- denotes )
T2912 2547-2549 TO denotes To
T2913 2550-2558 VB denotes increase
T2914 2559-2571 NN denotes palatability
T2915 2572-2575 CD denotes 0.2
T2916 2575-2576 NN denotes %
T2917 2577-2584 NN denotes sucrose
T2918 2585-2588 VB denotes was
T2919 2589-2594 VB denotes added
T2920 2595-2597 TO denotes to
T2921 2598-2601 DT denotes the
T2922 2602-2610 NN denotes drinking
T2923 2611-2616 NN denotes water
T2924 2617-2619 IN denotes of
T2925 2620-2623 DT denotes all
T2926 2624-2630 NN denotes groups
T2927 2631-2632 -LRB- denotes (
T2928 2632-2635 NNP denotes Van
T2929 2636-2639 NNP denotes der
T2930 2640-2645 NNP denotes Waaji
T2931 2646-2648 FW denotes et
T2932 2649-2652 FW denotes al.
T2933 2652-2653 -COMMA- denotes ,
T2934 2654-2655 -LRB- denotes [
T2935 2655-2657 CD denotes 23
T2936 2657-2658 -RRB- denotes ]
T2937 2658-2659 -RRB- denotes )
T2938 2661-2666 JJ denotes Fresh
T2939 2667-2678 NN denotes carrageenan
T2940 2679-2688 NN denotes solutions
T2941 2689-2693 VB denotes were
T2942 2694-2702 VB denotes prepared
T2943 2703-2708 RB denotes daily
T3130 2711-2721 NN denotes Evaluation
T3131 2722-2724 IN denotes of
T3132 2725-2732 NN denotes Colitis
T3133 2733-2737 NNP denotes Body
T3134 2738-2744 NN denotes weight
T3135 2744-2745 -COMMA- denotes ,
T3136 2746-2752 NN denotes liquid
T3137 2753-2756 CC denotes and
T3138 2757-2761 NN denotes food
T3139 2762-2773 NN denotes consumption
T3140 2773-2774 -COMMA- denotes ,
T3141 2775-2783 NN denotes diarrhea
T3142 2784-2787 CC denotes and
T3143 2788-2794 JJ denotes rectal
T3144 2795-2803 NN denotes bleeding
T3145 2804-2805 -LRB- denotes (
T3146 2805-2813 VB denotes detected
T3147 2814-2816 IN denotes by
T3148 2817-2820 NN denotes eye
T3149 2821-2831 NN denotes inspection
T3150 2831-2832 -RRB- denotes )
T3151 2833-2837 VB denotes were
T3152 2838-2846 VB denotes recorded
T3153 2847-2857 IN denotes throughout
T3154 2858-2861 DT denotes the
T3155 2862-2869 NN denotes feeding
T3156 2870-2876 NN denotes period
T3157 2878-2883 IN denotes After
T3158 2884-2886 CD denotes 55
T3159 2887-2891 NN denotes days
T3160 2891-2892 -COMMA- denotes ,
T3161 2893-2900 NN denotes animals
T3162 2901-2905 VB denotes were
T3163 2906-2916 VB denotes sacrificed
T3164 2917-2919 IN denotes by
T3165 2920-2928 JJ denotes cervical
T3166 2929-2940 NN denotes dislocation
T3167 2942-2945 DT denotes The
T3168 2946-2952 NN denotes length
T3169 2953-2955 IN denotes of
T3170 2956-2959 DT denotes the
T3171 2960-2965 NN denotes colon
T3172 2966-2969 VB denotes was
T3173 2970-2978 VB denotes measured
T3174 2979-2981 IN denotes as
T3175 2982-2991 VB denotes described
T3176 2992-2994 IN denotes by
T3177 2995-3003 NNP denotes Okayashu
T3178 3004-3006 FW denotes et
T3179 3007-3010 FW denotes al.
T3180 3011-3012 -LRB- denotes [
T3181 3012-3014 CD denotes 24
T3182 3014-3015 -RRB- denotes ]
T3183 3017-3021 RB denotes Then
T3184 3021-3022 -COMMA- denotes ,
T3185 3023-3027 DT denotes each
T3186 3028-3033 NN denotes colon
T3187 3034-3037 VB denotes was
T3188 3038-3045 VB denotes ligated
T3189 3046-3048 IN denotes in
T3190 3049-3057 NN denotes sections
T3191 3058-3060 IN denotes of
T3192 3061-3062 CD denotes 2
T3193 3063-3065 NN denotes cm
T3194 3066-3069 CC denotes and
T3195 3070-3071 CD denotes 1
T3196 3072-3074 TO denotes to
T3197 3075-3076 CD denotes 2
T3198 3077-3079 NN denotes ml
T3199 3080-3082 IN denotes of
T3200 3083-3085 CD denotes 10
T3201 3085-3086 NN denotes %
T3202 3087-3095 NN denotes formalin
T3203 3096-3099 VB denotes was
T3204 3100-3107 VB denotes infused
T3205 3108-3112 IN denotes into
T3206 3113-3116 DT denotes the
T3207 3117-3127 JJ denotes intestinal
T3208 3128-3133 NN denotes lumen
T3209 3135-3138 DT denotes The
T3210 3139-3149 RB denotes moderately
T3211 3150-3159 VB denotes distended
T3212 3160-3167 NN denotes segment
T3213 3168-3171 VB denotes was
T3214 3172-3181 VB denotes sectioned
T3215 3182-3185 CC denotes and
T3216 3186-3191 VB denotes fixed
T3217 3192-3194 IN denotes in
T3218 3195-3197 CD denotes 10
T3219 3197-3198 NN denotes %
T3220 3199-3207 NN denotes formalin
T3221 3209-3212 DT denotes The
T3222 3213-3222 VB denotes following
T3223 3223-3226 NN denotes day
T3224 3226-3227 -COMMA- denotes ,
T3225 3228-3231 DT denotes the
T3226 3232-3242 JJ denotes intestinal
T3227 3243-3250 NN denotes content
T3228 3251-3254 VB denotes was
T3229 3255-3262 VB denotes removed
T3230 3263-3265 IN denotes by
T3231 3266-3275 NN denotes vortexing
T3232 3277-3280 DT denotes The
T3233 3281-3286 VB denotes fixed
T3234 3287-3294 NN denotes segment
T3235 3295-3298 VB denotes was
T3236 3299-3303 VB denotes kept
T3237 3304-3306 IN denotes in
T3238 3307-3309 CD denotes 10
T3239 3309-3310 NN denotes %
T3240 3311-3319 NN denotes formalin
T3241 3320-3322 IN denotes at
T3242 3323-3326 NN denotes 4°C
T3243 3327-3332 IN denotes until
T3244 3333-3336 DT denotes the
T3245 3337-3345 NN denotes paraffin
T3246 3346-3355 NN denotes embedding
T3247 3356-3365 NN denotes procedure
T3248 3367-3369 TO denotes To
T3249 3370-3378 VB denotes evaluate
T3250 3379-3382 DT denotes the
T3251 3383-3389 NN denotes degree
T3252 3390-3392 IN denotes of
T3253 3393-3405 NN denotes inflammation
T3254 3405-3406 -COMMA- denotes ,
T3255 3407-3411 DT denotes this
T3256 3412-3419 NN denotes segment
T3257 3420-3422 IN denotes of
T3258 3423-3428 NN denotes colon
T3259 3429-3432 VB denotes was
T3260 3433-3439 VB denotes opened
T3261 3440-3454 RB denotes longitudinally
T3262 3455-3458 CC denotes and
T3263 3459-3470 JJ denotes macroscopic
T3264 3471-3474 CC denotes and
T3265 3475-3487 JJ denotes histological
T3266 3488-3494 NN denotes scores
T3267 3495-3497 IN denotes of
T3268 3498-3510 NN denotes inflammation
T3269 3511-3515 VB denotes were
T3270 3516-3524 VB denotes recorded
T3271 3525-3527 IN denotes as
T3272 3528-3538 RB denotes previously
T3273 3539-3548 VB denotes described
T3274 3549-3550 -LRB- denotes [
T3275 3550-3552 CD denotes 25
T3276 3552-3553 -RRB- denotes ]
T3277 3553-3554 -COMMA- denotes ,
T3278 3555-3556 -LRB- denotes [
T3279 3556-3558 CD denotes 26
T3280 3558-3559 -RRB- denotes ]
T3281 3561-3564 DT denotes The
T3282 3565-3574 NN denotes toluidine
T3283 3575-3579 JJ denotes blue
T3284 3580-3588 NN denotes staining
T3285 3589-3592 VB denotes was
T3286 3593-3597 VB denotes used
T3287 3598-3601 IN denotes for
T3288 3602-3616 NN denotes identification
T3289 3617-3619 IN denotes of
T3290 3620-3629 VB denotes sulphated
T3291 3630-3645 NN denotes polysaccharides
T3292 3646-3648 IN denotes in
T3293 3649-3652 DT denotes the
T3294 3653-3663 JJ denotes intestinal
T3295 3664-3670 NN denotes mucosa
T3296 3672-3674 IN denotes On
T3297 3675-3678 DT denotes the
T3298 3679-3682 NN denotes day
T3299 3683-3685 IN denotes of
T3300 3686-3695 NN denotes sacrifice
T3301 3695-3696 -COMMA- denotes ,
T3302 3697-3698 DT denotes a
T3303 3699-3704 JJ denotes fresh
T3304 3705-3711 NN denotes sample
T3305 3712-3714 IN denotes of
T3306 3715-3719 DT denotes each
T3307 3720-3725 NN denotes colon
T3308 3726-3727 -LRB- denotes (
T3309 3727-3729 CD denotes 50
T3310 3730-3732 NN denotes mg
T3311 3732-3733 -RRB- denotes )
T3312 3734-3737 VB denotes was
T3313 3738-3747 VB denotes collected
T3314 3748-3751 IN denotes for
T3315 3752-3767 NN denotes myeloperoxidase
T3316 3768-3769 -LRB- denotes (
T3317 3769-3772 NN denotes MPO
T3318 3772-3773 -RRB- denotes )
T3319 3774-3779 NN denotes assay
T3320 3780-3789 VB denotes according
T3321 3790-3792 TO denotes to
T3322 3793-3800 NNP denotes Krawisz
T3323 3801-3803 FW denotes et
T3324 3804-3807 FW denotes al.
T3325 3807-3808 -COMMA- denotes ,
T3326 3809-3810 -LRB- denotes [
T3327 3810-3812 CD denotes 27
T3328 3812-3813 -RRB- denotes ]
T3329 3815-3818 DT denotes The
T3330 3819-3824 NN denotes level
T3331 3825-3827 IN denotes of
T3332 3828-3831 NN denotes MPO
T3333 3831-3832 -COMMA- denotes ,
T3334 3833-3839 RB denotes mainly
T3335 3840-3849 VB denotes expressed
T3336 3850-3852 IN denotes by
T3337 3853-3864 NN denotes neutrophils
T3338 3864-3865 -COMMA- denotes ,
T3339 3866-3875 VB denotes indicates
T3340 3876-3879 DT denotes the
T3341 3880-3884 NN denotes rate
T3342 3885-3887 IN denotes of
T3343 3888-3899 NN denotes recruitment
T3344 3900-3902 IN denotes of
T3345 3903-3914 NN denotes neutrophils
T3346 3915-3917 TO denotes to
T3347 3918-3921 DT denotes the
T3348 3922-3932 JJ denotes intestinal
T3349 3933-3939 NN denotes mucosa
T3350 3941-3944 CD denotes One
T3351 3945-3949 NN denotes unit
T3352 3950-3952 IN denotes of
T3353 3953-3956 NN denotes MPO
T3354 3957-3965 NN denotes activity
T3355 3966-3977 VB denotes corresponds
T3356 3978-3980 TO denotes to
T3357 3981-3984 DT denotes the
T3358 3985-3996 NN denotes degradation
T3359 3997-3999 IN denotes of
T3360 4000-4001 CD denotes 1
T3361 4002-4006 NN denotes µmol
T3362 4007-4009 IN denotes of
T3363 4010-4018 NN denotes peroxide
T3364 4019-4022 IN denotes per
T3365 4023-4029 NN denotes minute
T3366 4030-4032 IN denotes at
T3367 4033-4037 NN denotes 25°C
T3676 4040-4044 NNP denotes Cell
T3677 4045-4052 NNP denotes Culture
T3678 4053-4056 DT denotes All
T3679 4057-4063 NN denotes tissue
T3680 4064-4071 NN denotes culture
T3681 4072-4080 NN denotes reagents
T3682 4081-4085 VB denotes were
T3683 4086-4090 IN denotes from
T3684 4091-4101 NN denotes Invitrogen
T3685 4102-4103 -LRB- denotes (
T3686 4103-4108 NNP denotes Cergy
T3687 4109-4117 NNP denotes Pontoise
T3688 4117-4118 -COMMA- denotes ,
T3689 4119-4125 NNP denotes France
T3690 4125-4126 -RRB- denotes )
T3691 4128-4133 NN denotes THP-1
T3692 4134-4139 JJ denotes human
T3693 4140-4149 JJ denotes monocytic
T3694 4150-4155 NN denotes cells
T3695 4156-4160 VB denotes were
T3696 4161-4171 VB denotes maintained
T3697 4172-4174 IN denotes in
T3698 4175-4184 NN denotes RPMI-1640
T3699 4185-4197 VB denotes supplemented
T3700 4198-4202 IN denotes with
T3701 4203-4205 CD denotes 10
T3702 4205-4206 NN denotes %
T3703 4207-4210 NN denotes FCS
T3704 4210-4211 -COMMA- denotes ,
T3705 4212-4213 CD denotes 2
T3706 4214-4216 NN denotes mM
T3707 4217-4218 NN denotes L
T3708 4219-4229 NN denotes -glutamine
T3709 4229-4230 -COMMA- denotes ,
T3710 4231-4233 CD denotes 50
T3711 4234-4238 NN denotes U/ml
T3712 4239-4249 NN denotes penicillin
T3713 4250-4253 CC denotes and
T3714 4254-4256 CD denotes 50
T3715 4257-4262 NN denotes mg/ml
T3716 4263-4275 NN denotes streptomycin
T3717 4276-4278 IN denotes at
T3718 4279-4283 NN denotes 37°C
T3719 4284-4286 IN denotes in
T3720 4287-4288 DT denotes a
T3721 4289-4290 CD denotes 5
T3722 4290-4291 NN denotes %
T3723 4292-4295 NN denotes CO2
T3724 4296-4305 NN denotes incubator
T3725 4307-4312 JJ denotes Human
T3726 4313-4323 JJ denotes peripheral
T3727 4324-4329 NN denotes blood
T3728 4330-4341 JJ denotes mononuclear
T3729 4342-4347 NN denotes cells
T3730 4348-4352 VB denotes were
T3731 4353-4361 VB denotes obtained
T3732 4362-4366 IN denotes from
T3733 4367-4378 VB denotes heparinized
T3734 4379-4384 NN denotes blood
T3735 4385-4387 IN denotes by
T3736 4388-4402 JJ denotes Ficoll-Hypaque
T3737 4403-4410 NN denotes density
T3738 4411-4419 NN denotes gradient
T3739 4421-4430 NN denotes Monocytes
T3740 4431-4435 VB denotes were
T3741 4436-4440 RB denotes then
T3742 4441-4449 VB denotes isolated
T3743 4450-4452 IN denotes by
T3744 4453-4462 NN denotes adherence
T3745 4463-4465 TO denotes to
T3746 4466-4473 NN denotes culture
T3747 4474-4480 NN denotes flasks
T3748 4481-4483 IN denotes as
T3749 4484-4493 VB denotes described
T3750 4494-4495 -LRB- denotes [
T3751 4495-4497 CD denotes 28
T3752 4497-4498 -RRB- denotes ]
T3753 4500-4503 IN denotes For
T3754 4504-4508 NN denotes cell
T3755 4509-4520 NN denotes aggregation
T3756 4520-4521 -COMMA- denotes ,
T3757 4522-4531 NN denotes monocytes
T3758 4532-4536 VB denotes were
T3759 4537-4545 VB denotes cultured
T3760 4546-4548 IN denotes in
T3761 4549-4552 DT denotes the
T3762 4553-4561 NN denotes presence
T3763 4562-4564 CC denotes or
T3764 4565-4572 NN denotes absence
T3765 4573-4575 IN denotes of
T3766 4576-4579 NN denotes C10
T3767 4580-4582 CC denotes or
T3768 4583-4586 NN denotes C40
T3769 4587-4590 IN denotes for
T3770 4591-4593 CD denotes 72
T3771 4594-4595 NN denotes h
T3772 4597-4601 NN denotes Cell
T3773 4602-4610 NN denotes colonies
T3774 4611-4615 VB denotes were
T3775 4616-4625 VB denotes monitored
T3776 4626-4631 IN denotes under
T3777 4632-4634 DT denotes an
T3778 4635-4643 VB denotes inverted
T3779 4644-4649 NN denotes phase
T3780 4650-4658 NN denotes contrast
T3781 4659-4669 NN denotes microscope
T3782 4670-4677 VB denotes coupled
T3783 4678-4685 IN denotes through
T3784 4686-4687 DT denotes a
T3785 4688-4693 NN denotes video
T3786 4694-4700 NN denotes camera
T3787 4701-4703 TO denotes to
T3788 4704-4705 DT denotes a
T3789 4706-4714 NN denotes computer
T3790 4716-4718 IN denotes In
T3791 4719-4723 DT denotes some
T3792 4724-4729 NN denotes wells
T3793 4729-4730 -COMMA- denotes ,
T3794 4731-4743 VB denotes neutralizing
T3795 4744-4754 JJ denotes monoclonal
T3796 4755-4763 NN denotes antibody
T3797 4764-4766 TO denotes to
T3798 4767-4773 NN denotes ICAM-1
T3799 4774-4775 -LRB- denotes (
T3800 4775-4778 CD denotes 2.5
T3801 4779-4784 NN denotes µg/ml
T3802 4784-4785 -RRB- denotes )
T3803 4786-4787 -LRB- denotes (
T3804 4787-4791 NNP denotes Tebu
T3805 4791-4792 -COMMA- denotes ,
T3806 4793-4795 NNP denotes Le
T3807 4796-4802 NNP denotes Perray
T3808 4803-4805 NNP denotes en
T3809 4806-4814 NNP denotes Yvelines
T3810 4814-4815 -COMMA- denotes ,
T3811 4816-4822 NNP denotes France
T3812 4822-4823 -RRB- denotes )
T3813 4824-4827 VB denotes was
T3814 4828-4833 VB denotes added
T3986 4836-4840 NN denotes Cell
T3987 4841-4846 NN denotes Cycle
T3988 4847-4855 NN denotes Analysis
T3989 4856-4861 NN denotes THP-1
T3990 4862-4867 NN denotes cells
T3991 4868-4870 IN denotes in
T3992 4871-4882 JJ denotes exponential
T3993 4883-4889 NN denotes growth
T3994 4890-4895 NN denotes phase
T3995 4896-4900 VB denotes were
T3996 4901-4908 VB denotes exposed
T3997 4909-4911 TO denotes to
T3998 4912-4920 JJ denotes complete
T3999 4921-4927 NN denotes medium
T4000 4928-4930 IN denotes in
T4001 4931-4934 DT denotes the
T4002 4935-4943 NN denotes presence
T4003 4944-4946 CC denotes or
T4004 4947-4954 NN denotes absence
T4005 4955-4957 IN denotes of
T4006 4958-4970 NN denotes carrageenans
T4007 4971-4974 IN denotes for
T4008 4975-4977 CD denotes 24
T4009 4978-4979 NN denotes h
T4010 4980-4986 IN denotes before
T4011 4987-4992 VB denotes being
T4012 4993-5000 VB denotes stained
T4013 5001-5005 IN denotes with
T4014 5006-5015 NN denotes propidium
T4015 5016-5022 NN denotes iodide
T4016 5023-5028 VB denotes using
T4017 5029-5032 DT denotes the
T4018 5033-5041 JJ denotes DNA-Prep
T4019 5042-5049 NNP denotes Coulter
T4020 5050-5053 NN denotes kit
T4021 5054-5063 VB denotes according
T4022 5064-5066 TO denotes to
T4023 5067-5070 DT denotes the
T4024 5071-5083 NN denotes manufacturer
T4025 5083-5085 POS denotes 's
T4026 5086-5097 NN denotes instruction
T4027 5098-5099 -LRB- denotes (
T4028 5099-5114 NNP denotes Beckman-Coulter
T4029 5114-5115 -COMMA- denotes ,
T4030 5116-5126 NNP denotes Villepinte
T4031 5126-5127 -COMMA- denotes ,
T4032 5128-5134 NNP denotes France
T4033 5134-5135 -RRB- denotes )
T4034 5137-5141 NN denotes Cell
T4035 5142-5145 NN denotes DNA
T4036 5146-5153 NN denotes content
T4037 5154-5157 VB denotes was
T4038 5158-5162 RB denotes then
T4039 5163-5171 VB denotes analyzed
T4040 5172-5174 IN denotes by
T4041 5175-5179 NN denotes flow
T4042 5180-5189 NN denotes cytometry
T4043 5190-5195 VB denotes using
T4044 5196-5198 DT denotes an
T4045 5199-5204 NN denotes EPICS
T4046 5205-5208 NN denotes XL2
T4047 5209-5210 -LRB- denotes (
T4048 5210-5225 NN denotes Beckman-Coulter
T4049 5225-5226 -RRB- denotes )
T4050 5228-5231 NN denotes Raw
T4051 5232-5236 NN denotes data
T4052 5237-5240 IN denotes for
T4053 5241-5244 DT denotes the
T4054 5245-5257 NN denotes distribution
T4055 5258-5260 IN denotes of
T4056 5261-5264 NN denotes DNA
T4057 5265-5272 NN denotes content
T4058 5273-5275 IN denotes of
T4059 5276-5282 CD denotes 30,000
T4060 5283-5288 NN denotes cells
T4061 5289-5298 VB denotes retrieved
T4062 5299-5303 IN denotes from
T4063 5304-5307 DT denotes the
T4064 5308-5317 NN denotes cytometer
T4065 5318-5322 VB denotes were
T4066 5323-5332 VB denotes expressed
T4067 5333-5335 IN denotes as
T4068 5336-5339 DT denotes the
T4069 5340-5350 NN denotes percentage
T4070 5351-5353 IN denotes of
T4071 5354-5359 NN denotes G0/G1
T4072 5360-5367 IN denotes through
T4073 5368-5372 NN denotes G2/M
T4074 5373-5384 NN denotes populations
T4075 5386-5396 NNP denotes Multicycle
T4076 5397-5399 NN denotes AV
T4077 5400-5408 NN denotes software
T4078 5409-5410 -LRB- denotes (
T4079 5410-5417 NNP denotes Phoenix
T4080 5418-5422 NNP denotes Flow
T4081 5423-5430 NNP denotes Systems
T4082 5430-5431 -COMMA- denotes ,
T4083 5432-5435 NNP denotes San
T4084 5436-5441 NNP denotes Diego
T4085 5441-5442 -COMMA- denotes ,
T4086 5443-5445 NN denotes CA
T4087 5445-5446 -RRB- denotes )
T4088 5447-5450 VB denotes was
T4089 5451-5455 VB denotes used
T4090 5456-5458 TO denotes to
T4091 5459-5467 VB denotes generate
T4092 5468-5471 NN denotes DNA
T4093 5472-5479 NN denotes content
T4094 5480-5489 NN denotes frequency
T4095 5490-5500 NN denotes histograms
T4096 5501-5504 CC denotes and
T4097 5505-5515 VB denotes facilitate
T4098 5516-5520 NN denotes data
T4099 5521-5529 NN denotes analysis
T4242 5532-5536 NNP denotes Cell
T4243 5537-5544 NNP denotes Surface
T4244 5545-5552 NNP denotes Antigen
T4245 5553-5563 NN denotes Expression
T4246 5564-5572 NN denotes Analysis
T4247 5573-5583 JJ denotes Peripheral
T4248 5584-5589 NN denotes Blood
T4249 5590-5599 NN denotes Monocytes
T4250 5600-5602 CC denotes or
T4251 5603-5608 NN denotes THP-1
T4252 5609-5614 NN denotes cells
T4253 5615-5619 VB denotes were
T4254 5620-5627 VB denotes exposed
T4255 5628-5630 TO denotes to
T4256 5631-5639 JJ denotes complete
T4257 5640-5646 NN denotes medium
T4258 5647-5649 IN denotes in
T4259 5650-5653 DT denotes the
T4260 5654-5662 NN denotes presence
T4261 5663-5665 CC denotes or
T4262 5666-5673 NN denotes absence
T4263 5674-5676 IN denotes of
T4264 5677-5688 NN denotes carrageenan
T4265 5689-5692 IN denotes for
T4266 5693-5695 CD denotes 36
T4267 5696-5697 NN denotes h
T4268 5699-5704 IN denotes After
T4269 5705-5708 CD denotes two
T4270 5709-5715 NN denotes washes
T4271 5716-5718 IN denotes in
T4272 5719-5722 NNP denotes PBS
T4273 5723-5730 IN denotes without
T4274 5731-5735 NN denotes Ca2+
T4275 5736-5739 CC denotes and
T4276 5740-5744 NN denotes Mg2+
T4277 5744-5745 -COMMA- denotes ,
T4278 5746-5751 NN denotes cells
T4279 5752-5756 VB denotes were
T4280 5757-5766 VB denotes incubated
T4281 5767-5769 IN denotes in
T4282 5770-5773 NN denotes PBS
T4283 5774-5784 VB denotes containing
T4284 5785-5788 CD denotes 0.1
T4285 5788-5789 NN denotes %
T4286 5790-5797 NN denotes gelatin
T4287 5798-5801 CC denotes and
T4288 5802-5803 CD denotes 8
T4289 5803-5804 NN denotes %
T4290 5805-5807 NN denotes AB
T4291 5808-5813 JJ denotes human
T4292 5814-5819 NN denotes serum
T4293 5820-5822 TO denotes to
T4294 5823-5830 VB denotes prevent
T4295 5831-5838 NN denotes binding
T4296 5839-5841 TO denotes to
T4297 5842-5844 NN denotes Fc
T4298 5845-5854 NN denotes receptors
T4299 5856-5860 RB denotes Then
T4300 5860-5861 -COMMA- denotes ,
T4301 5862-5867 NN denotes 5×105
T4302 5868-5873 NN denotes cells
T4303 5874-5878 VB denotes were
T4304 5879-5888 VB denotes incubated
T4305 5889-5893 IN denotes with
T4306 5894-5901 JJ denotes primary
T4307 5902-5912 NN denotes antibodies
T4308 5913-5915 IN denotes at
T4309 5916-5919 NN denotes 4°C
T4310 5920-5923 IN denotes for
T4311 5924-5926 CD denotes 30
T4312 5927-5930 NN denotes min
T4313 5932-5935 CD denotes Two
T4314 5936-5941 JJ denotes other
T4315 5942-5948 NN denotes washes
T4316 5949-5951 IN denotes in
T4317 5952-5955 NNP denotes PBS
T4318 5956-5964 VB denotes preceded
T4319 5965-5975 NN denotes incubation
T4320 5976-5980 IN denotes with
T4321 5981-5996 JJ denotes FITC-conjugated
T4322 5997-6001 NN denotes goat
T4323 6002-6010 NN denotes antibody
T4324 6011-6021 JJ denotes anti-mouse
T4325 6022-6025 NN denotes IgG
T4326 6026-6033 VB denotes diluted
T4327 6034-6040 CD denotes 1/1000
T4328 6041-6043 IN denotes at
T4329 6044-6047 NN denotes 4°C
T4330 6048-6051 IN denotes for
T4331 6052-6054 CD denotes 30
T4332 6055-6058 NN denotes min
T4333 6059-6060 -LRB- denotes (
T4334 6060-6064 NN denotes Tebu
T4335 6064-6065 -RRB- denotes )
T4336 6067-6072 IN denotes After
T4337 6073-6076 CD denotes two
T4338 6077-6087 JJ denotes additional
T4339 6088-6094 NN denotes washes
T4340 6094-6095 -COMMA- denotes ,
T4341 6096-6104 NN denotes analysis
T4342 6105-6107 IN denotes of
T4343 6108-6115 VB denotes stained
T4344 6116-6121 NN denotes cells
T4345 6122-6125 VB denotes was
T4346 6126-6135 VB denotes performed
T4347 6136-6138 IN denotes on
T4348 6139-6141 DT denotes an
T4349 6142-6147 NN denotes EPICS
T4350 6148-6151 NN denotes XL2
T4351 6152-6153 -LRB- denotes (
T4352 6153-6168 NN denotes Beckman-Coulter
T4353 6168-6169 -RRB- denotes )
T4354 6171-6174 DT denotes The
T4355 6175-6179 NN denotes cell
T4356 6180-6190 NN denotes population
T4357 6191-6194 VB denotes was
T4358 6195-6200 VB denotes gated
T4359 6201-6210 VB denotes according
T4360 6211-6213 TO denotes to
T4361 6214-6217 PRP-DOLLAR- denotes its
T4362 6218-6225 JJ denotes forward
T4363 6226-6229 CC denotes and
T4364 6230-6240 JJ denotes wide-angle
T4365 6241-6246 NN denotes light
T4366 6247-6257 NN denotes scattering
T4367 6259-6263 NN denotes Data
T4368 6264-6268 VB denotes were
T4369 6269-6278 VB denotes expressed
T4370 6279-6281 IN denotes as
T4371 6282-6286 JJ denotes mean
T4372 6287-6295 JJ denotes relative
T4373 6296-6308 NN denotes fluorescence
T4374 6309-6318 NN denotes intensity
T4375 6319-6320 -LRB- denotes (
T4376 6320-6323 NN denotes MFI
T4377 6323-6324 -RRB- denotes )
T4378 6325-6327 IN denotes of
T4379 6328-6332 CD denotes 3000
T4380 6333-6338 NN denotes cells
T4587 6341-6344 NN denotes TNF
T4588 6345-6353 NNP denotes Activity
T4589 6354-6362 NNP denotes Bioassay
T4590 6363-6372 NN denotes Monocytes
T4591 6373-6375 CC denotes or
T4592 6376-6381 NN denotes THP-1
T4593 6382-6387 NN denotes cells
T4594 6388-6392 VB denotes were
T4595 6393-6401 VB denotes cultured
T4596 6402-6406 IN denotes with
T4597 6407-6409 CC denotes or
T4598 6410-6417 IN denotes without
T4599 6418-6427 JJ denotes different
T4600 6428-6442 NN denotes concentrations
T4601 6443-6445 IN denotes of
T4602 6446-6450 NN denotes CGNs
T4603 6451-6453 CC denotes or
T4604 6454-6457 NN denotes LPS
T4605 6458-6459 -LRB- denotes (
T4606 6459-6469 NN denotes Salmonella
T4607 6470-6477 NN denotes typhosa
T4608 6477-6478 -COMMA- denotes ,
T4609 6479-6484 NNP denotes Sigma
T4610 6484-6485 -RRB- denotes )
T4611 6486-6489 IN denotes for
T4612 6490-6492 CD denotes 24
T4613 6493-6494 NN denotes h
T4614 6495-6497 CC denotes or
T4615 6498-6501 DT denotes the
T4616 6502-6511 VB denotes indicated
T4617 6512-6516 NN denotes time
T4618 6518-6530 RB denotes Biologically
T4619 6531-6537 JJ denotes active
T4620 6538-6545 NN denotes TNF-α/β
T4621 6546-6548 IN denotes in
T4622 6549-6555 NN denotes tissue
T4623 6556-6563 NN denotes culture
T4624 6564-6575 NN denotes supernatant
T4625 6576-6579 VB denotes was
T4626 6580-6588 VB denotes measured
T4627 6589-6594 VB denotes using
T4628 6595-6598 DT denotes the
T4629 6599-6603 NN denotes WEHI
T4630 6604-6607 CD denotes 164
T4631 6608-6613 NN denotes clone
T4632 6614-6621 NN denotes 13-cell
T4633 6622-6629 NN denotes killing
T4634 6630-6635 NN denotes assay
T4635 6636-6637 -LRB- denotes [
T4636 6637-6639 CD denotes 29
T4637 6639-6640 -RRB- denotes ]
T4638 6642-6645 NN denotes TNF
T4639 6646-6660 NN denotes concentrations
T4640 6661-6664 VB denotes are
T4641 6665-6674 VB denotes expressed
T4642 6675-6677 IN denotes as
T4643 6678-6683 NN denotes pg/ml
T4750 6686-6692 NN denotes RT-PCR
T4751 6693-6701 NN denotes Analysis
T4752 6702-6707 JJ denotes Total
T4753 6708-6711 NN denotes RNA
T4754 6712-6716 IN denotes from
T4755 6717-6726 NN denotes monocytes
T4756 6727-6730 VB denotes was
T4757 6731-6739 VB denotes isolated
T4758 6740-6745 VB denotes using
T4759 6746-6752 NN denotes TRIzol
T4760 6753-6761 NN denotes Reagent™
T4761 6762-6763 -LRB- denotes (
T4762 6763-6773 NN denotes Invitrogen
T4763 6773-6774 -RRB- denotes )
T4764 6774-6778 NN denotes . cD
T4765 6778-6781 VB denotes NA
T4766 6781-6790 VB denotes was gener
T4767 6790-6792 IN denotes at
T4768 6792-6793 CD denotes e
T4769 6793-6795 NN denotes d
T4770 6795-6797 IN denotes on
T4771 6798-6803 JJ denotes 1 µg
T4772 6803-6806 NN denotes of
T4773 6806-6808 IN denotes to
T4774 6808-6809 DT denotes t
T4775 6809-6817 NN denotes al RNA i
T4776 6817-6823 NN denotes n a re
T4777 6823-6825 IN denotes ac
T4778 6825-6827 CD denotes ti
T4779 6827-6829 NN denotes on
T4780 6830-6831 -COMMA- denotes v
T4781 6831-6836 VB denotes olume
T4782 6837-6842 NN denotes of 20
T4783 6843-6850 JJ denotes µl, usi
T4784 6850-6863 NN denotes ng M-MLV reve
T4785 6863-6864 -LRB- denotes r
T4786 6864-6874 NN denotes se transcr
T4787 6874-6875 -RRB- denotes i
T4788 6895-6898 NN denotes PCR
T4789 6899-6902 VB denotes was
T4790 6903-6907 VB denotes done
T4791 6908-6910 IN denotes in
T4792 6911-6914 DT denotes the
T4793 6915-6921 JJ denotes linear
T4794 6922-6927 NN denotes range
T4795 6928-6930 IN denotes of
T4796 6931-6944 NN denotes amplification
T4797 6945-6946 -LRB- denotes (
T4798 6946-6956 VB denotes determined
T4799 6957-6960 IN denotes for
T4800 6961-6965 DT denotes each
T4801 6966-6972 NN denotes primer
T4802 6973-6982 NN denotes pair-cDNA
T4803 6983-6994 NN denotes combination
T4804 6994-6995 -RRB- denotes )
T4805 6997-7005 JJ denotes Standard
T4806 7006-7009 NN denotes PCR
T4807 7010-7019 NN denotes reactions
T4808 7020-7024 VB denotes were
T4809 7025-7034 VB denotes performed
T4810 7035-7039 IN denotes with
T4811 7040-7041 CD denotes 1
T4812 7042-7044 NN denotes µl
T4813 7045-7047 IN denotes of
T4814 7048-7051 DT denotes the
T4815 7052-7056 NN denotes cDNA
T4816 7057-7065 NN denotes solution
T4817 7065-7066 -COMMA- denotes ,
T4818 7067-7069 CD denotes 50
T4819 7070-7072 NN denotes µM
T4820 7073-7075 IN denotes of
T4821 7076-7080 DT denotes each
T4822 7081-7087 NN denotes primer
T4823 7088-7096 NN denotes solution
T4824 7096-7097 -COMMA- denotes ,
T4825 7098-7100 CD denotes 10
T4826 7101-7103 NN denotes mM
T4827 7104-7106 IN denotes of
T4828 7107-7111 DT denotes each
T4829 7112-7116 NN denotes dNTP
T4830 7116-7117 -COMMA- denotes ,
T4831 7118-7120 CD denotes 25
T4832 7121-7123 NN denotes mM
T4833 7124-7129 NN denotes MgCl2
T4834 7129-7130 -COMMA- denotes ,
T4835 7131-7134 NNP denotes 10X
T4836 7135-7143 NNP denotes Goldstar
T4837 7144-7147 NN denotes DNA
T4838 7148-7158 NN denotes polymerase
T4839 7159-7167 NN denotes reaction
T4840 7168-7174 NN denotes buffer
T4841 7174-7175 -COMMA- denotes ,
T4842 7176-7179 CC denotes and
T4843 7180-7183 CD denotes 0.5
T4844 7184-7189 NN denotes units
T4845 7190-7192 IN denotes of
T4846 7193-7201 NNP denotes Goldstar
T4847 7202-7205 NN denotes DNA
T4848 7206-7216 NN denotes polymerase
T4849 7217-7218 -LRB- denotes (
T4850 7218-7228 NNP denotes Eurogentec
T4851 7228-7229 -COMMA- denotes ,
T4852 7230-7237 NNP denotes Seraing
T4853 7237-7238 -COMMA- denotes ,
T4854 7239-7246 NNP denotes Belgium
T4855 7246-7247 -RRB- denotes )
T4856 7249-7254 JJ denotes First
T4857 7255-7258 NN denotes PCR
T4858 7259-7264 NN denotes cycle
T4859 7265-7274 VB denotes consisted
T4860 7275-7277 IN denotes of
T4861 7278-7279 CD denotes 1
T4862 7280-7283 NN denotes min
T4863 7284-7286 IN denotes at
T4864 7287-7291 NN denotes 92°C
T4865 7291-7292 -COMMA- denotes ,
T4866 7293-7294 CD denotes 1
T4867 7295-7298 NN denotes min
T4868 7299-7301 IN denotes at
T4869 7302-7306 NN denotes 58°C
T4870 7307-7310 CC denotes and
T4871 7311-7312 CD denotes 1
T4872 7313-7316 NN denotes min
T4873 7317-7319 IN denotes at
T4874 7320-7324 NN denotes 72°C
T4875 7324-7325 -COLON- denotes ;
T4876 7326-7330 RB denotes then
T4877 7331-7335 DT denotes each
T4878 7336-7339 NN denotes PCR
T4879 7340-7345 NN denotes cycle
T4880 7346-7355 VB denotes consisted
T4881 7356-7358 IN denotes of
T4882 7359-7361 CD denotes 40
T4883 7362-7365 NN denotes sec
T4884 7366-7368 IN denotes at
T4885 7369-7373 NN denotes 92°C
T4886 7373-7374 -COMMA- denotes ,
T4887 7375-7377 CD denotes 40
T4888 7378-7381 NN denotes sec
T4889 7382-7384 IN denotes at
T4890 7385-7389 NN denotes 58°C
T4891 7390-7393 CC denotes and
T4892 7394-7396 CD denotes 50
T4893 7397-7400 NN denotes sec
T4894 7401-7403 IN denotes at
T4895 7404-7409 NNP denotes 72°C.
T4896 7410-7414 NN denotes cDNA
T4897 7415-7418 IN denotes for
T4898 7419-7426 NN denotes β-actin
T4899 7427-7430 VB denotes was
T4900 7431-7440 VB denotes amplified
T4901 7441-7444 IN denotes for
T4902 7445-7447 CD denotes 28
T4903 7448-7454 NN denotes cycles
T4904 7455-7460 VB denotes using
T4905 7461-7464 DT denotes the
T4906 7465-7471 NN denotes oligos
T4907 7471-7472 -COLON- denotes :
T4908 7473-7478 NN denotes sense
T4909 7479-7504 NN denotes 5′-GGCATCGTGATGGACTCCG-3′
T4910 7505-7508 CC denotes and
T4911 7509-7518 JJ denotes antisense
T4912 7519-7544 JJ denotes 5′GCTGGAAGGTGGACAGCGA-3′.
T4913 7545-7549 NN denotes cDNA
T4914 7550-7553 IN denotes for
T4915 7554-7559 NN denotes TNF-α
T4916 7560-7563 VB denotes was
T4917 7564-7573 VB denotes amplified
T4918 7574-7577 IN denotes for
T4919 7578-7580 CD denotes 35
T4920 7581-7587 NN denotes cycles
T4921 7588-7593 VB denotes using
T4922 7594-7597 DT denotes the
T4923 7598-7604 NN denotes oligos
T4924 7604-7605 -COLON- denotes :
T4925 7606-7611 NN denotes sense
T4926 7612-7638 NN denotes 5′-AAGCCTGTAGCCCATGTTGT-3′
T4927 7639-7642 CC denotes and
T4928 7643-7652 JJ denotes antisense
T4929 7653-7680 JJ denotes 5′-CAGATAGATGGGCTCATACC-3′.
T4930 7681-7685 NN denotes cDNA
T4931 7686-7689 IN denotes for
T4932 7690-7696 NN denotes ICAM-1
T4933 7697-7700 VB denotes was
T4934 7701-7710 VB denotes amplified
T4935 7711-7714 IN denotes for
T4936 7715-7717 CD denotes 35
T4937 7718-7724 NN denotes cycles
T4938 7725-7730 VB denotes using
T4939 7731-7734 DT denotes the
T4940 7735-7741 NN denotes oligos
T4941 7742-7747 VB denotes sense
T4942 7748-7776 NN denotes 5′-GTAGCAGCCGCAGTCATAATGG-3′
T4943 7777-7780 CC denotes and
T4944 7781-7790 JJ denotes antisense
T4945 7791-7795 NN denotes 5′-A
T4946 7796-7820 NN denotes TGCTGTTGTATCTGACTGAGG-3′
T5239 7823-7828 NN denotes NF-kB
T5240 7829-7842 NN denotes Transcription
T5241 7843-7851 NNP denotes Reporter
T5242 7852-7856 NNP denotes Gene
T5243 7857-7862 NNP denotes Assay
T5244 7863-7866 DT denotes The
T5245 7867-7874 NN denotes plasmid
T5246 7875-7884 NN denotes 3XMHC-luc
T5247 7885-7886 -LRB- denotes (
T5248 7886-7887 DT denotes a
T5249 7888-7896 JJ denotes generous
T5250 7897-7901 NN denotes gift
T5251 7902-7906 IN denotes from
T5252 7907-7911 NNP denotes Drs.
T5253 7912-7914 NNP denotes J.
T5254 7915-7923 NNP denotes Westwick
T5255 7924-7927 CC denotes and
T5256 7928-7932 NNP denotes D.A.
T5257 7933-7940 NNP denotes Brenner
T5258 7940-7941 -COMMA- denotes ,
T5259 7942-7952 NNP denotes University
T5260 7953-7955 IN denotes of
T5261 7956-7961 NNP denotes North
T5262 7962-7970 NNP denotes Carolina
T5263 7970-7971 -COMMA- denotes ,
T5264 7972-7978 NNP denotes Chapel
T5265 7979-7983 NNP denotes Hill
T5266 7983-7984 -RRB- denotes )
T5267 7985-7993 VB denotes contains
T5268 7994-7999 CD denotes three
T5269 8000-8006 NN denotes copies
T5270 8007-8009 IN denotes of
T5271 8010-8026 JJ denotes NF-κB-responsive
T5272 8027-8034 NN denotes element
T5273 8035-8039 IN denotes from
T5274 8040-8043 DT denotes the
T5275 8044-8047 NN denotes MHC
T5276 8048-8053 NN denotes class
T5277 8054-8055 CD denotes I
T5278 8056-8061 NN denotes locus
T5279 8061-8062 -COMMA- denotes ,
T5280 8063-8069 VB denotes placed
T5281 8070-8078 RB denotes upstream
T5282 8079-8081 IN denotes of
T5283 8082-8085 DT denotes the
T5284 8086-8096 NN denotes luciferase
T5285 8097-8101 NN denotes gene
T5286 8103-8108 JJ denotes Human
T5287 8109-8118 JJ denotes monocytic
T5288 8119-8124 NN denotes THP-1
T5289 8125-8130 NN denotes cells
T5290 8131-8135 VB denotes were
T5291 8136-8147 RB denotes transiently
T5292 8148-8159 VB denotes transfected
T5293 8160-8162 IN denotes as
T5294 8163-8173 RB denotes previously
T5295 8174-8183 VB denotes described
T5296 8184-8185 -LRB- denotes [
T5297 8185-8187 CD denotes 30
T5298 8187-8188 -RRB- denotes ]
T5299 8188-8189 -COMMA- denotes ,
T5300 8190-8193 CC denotes and
T5301 8194-8198 RB denotes then
T5302 8199-8207 VB denotes cultured
T5303 8208-8211 IN denotes for
T5304 8212-8213 CD denotes 4
T5305 8214-8215 NN denotes h
T5306 8216-8221 RB denotes alone
T5307 8222-8224 CC denotes or
T5308 8225-8229 IN denotes with
T5309 8230-8240 VB denotes increasing
T5310 8241-8254 NN denotes concentration
T5311 8255-8257 IN denotes of
T5312 8258-8264 CC denotes either
T5313 8265-8268 NN denotes C10
T5314 8269-8271 CC denotes or
T5315 8272-8275 NN denotes C40
T5316 8277-8287 NN denotes Luciferase
T5317 8288-8296 NN denotes activity
T5318 8297-8300 VB denotes was
T5319 8301-8311 VB denotes determined
T5320 8312-8317 VB denotes using
T5321 8318-8319 DT denotes a
T5322 8320-8331 NN denotes luminometer
T5323 8332-8333 -LRB- denotes (
T5324 8333-8342 NN denotes Monolight
T5325 8343-8347 CD denotes 2010
T5326 8348-8359 NNP denotes Luminometer
T5327 8359-8360 -COMMA- denotes ,
T5328 8361-8364 NNP denotes Ann
T5329 8365-8370 NNP denotes Arbor
T5330 8370-8371 -COMMA- denotes ,
T5331 8372-8374 NNP denotes MI
T5332 8374-8375 -RRB- denotes )
T5492 8378-8385 NNP denotes Western
T5493 8386-8390 NNP denotes Blot
T5494 8391-8399 NN denotes Analysis
T5495 8400-8405 NN denotes THP-1
T5496 8406-8411 NN denotes cells
T5497 8412-8416 VB denotes were
T5498 8417-8427 VB denotes stimulated
T5499 8428-8431 IN denotes for
T5500 8432-8439 JJ denotes various
T5501 8440-8447 NN denotes lengths
T5502 8448-8450 IN denotes of
T5503 8451-8455 NN denotes time
T5504 8456-8460 IN denotes with
T5505 8461-8464 CD denotes 0.1
T5506 8465-8470 NN denotes mg/ml
T5507 8471-8474 NN denotes C10
T5508 8475-8477 CC denotes or
T5509 8478-8481 NN denotes C40
T5510 8481-8482 -COMMA- denotes ,
T5511 8483-8485 CC denotes or
T5512 8486-8488 CD denotes 10
T5513 8489-8494 NN denotes µg/ml
T5514 8495-8498 NN denotes LPS
T5515 8500-8505 NN denotes Cells
T5516 8506-8510 VB denotes were
T5517 8511-8515 RB denotes then
T5518 8516-8524 VB denotes pelleted
T5519 8524-8525 -COMMA- denotes ,
T5520 8526-8532 VB denotes washed
T5521 8533-8536 CC denotes and
T5522 8537-8548 VB denotes homogenised
T5523 8549-8551 IN denotes in
T5524 8552-8557 NN denotes lysis
T5525 8558-8564 NN denotes buffer
T5526 8565-8566 -LRB- denotes (
T5527 8566-8568 CD denotes 10
T5528 8569-8571 NN denotes mM
T5529 8572-8577 NNP denotes Hepes
T5530 8577-8578 -COMMA- denotes ,
T5531 8579-8581 NN denotes pH
T5532 8582-8585 CD denotes 7.9
T5533 8585-8586 -COMMA- denotes ,
T5534 8587-8590 CD denotes 150
T5535 8591-8593 NN denotes mM
T5536 8594-8598 NN denotes NaCl
T5537 8598-8599 -COMMA- denotes ,
T5538 8600-8601 CD denotes 1
T5539 8602-8604 NN denotes mM
T5540 8605-8609 NN denotes EDTA
T5541 8609-8610 -COMMA- denotes ,
T5542 8611-8614 CD denotes 0.6
T5543 8614-8615 NN denotes %
T5544 8616-8621 NN denotes NP-40
T5545 8621-8622 -COMMA- denotes ,
T5546 8623-8626 CC denotes and
T5547 8627-8630 CD denotes 0.5
T5548 8631-8633 NN denotes mM
T5549 8634-8638 NN denotes PMSF
T5550 8638-8639 -RRB- denotes )
T5551 8640-8642 IN denotes on
T5552 8643-8646 NN denotes ice
T5553 8648-8659 NN denotes Homogenates
T5554 8660-8664 VB denotes were
T5555 8665-8674 VB denotes sonicated
T5556 8674-8675 -COMMA- denotes ,
T5557 8676-8687 VB denotes centrifuged
T5558 8688-8690 IN denotes at
T5559 8691-8697 CD denotes 10,000
T5560 8698-8701 NN denotes rpm
T5561 8702-8704 TO denotes to
T5562 8705-8711 VB denotes remove
T5563 8712-8720 JJ denotes cellular
T5564 8721-8727 NN denotes debris
T5565 8727-8728 -COMMA- denotes ,
T5566 8729-8732 CC denotes and
T5567 8733-8744 NN denotes supernatant
T5568 8745-8754 VB denotes collected
T5569 8756-8763 NN denotes Protein
T5570 8764-8777 NN denotes concentration
T5571 8778-8781 VB denotes was
T5572 8782-8792 VB denotes determined
T5573 8793-8798 VB denotes using
T5574 8799-8802 DT denotes the
T5575 8803-8805 NN denotes DC
T5576 8806-8813 NN denotes Protein
T5577 8814-8819 NN denotes Assay
T5578 8820-8821 -LRB- denotes (
T5579 8821-8828 NN denotes Bio-Rad
T5580 8828-8829 -RRB- denotes )
T5581 8831-8839 NN denotes Proteins
T5582 8840-8842 IN denotes in
T5583 8843-8850 NN denotes samples
T5584 8851-8852 -LRB- denotes (
T5585 8852-8854 CD denotes 15
T5586 8855-8857 -COLON- denotes µg
T5587 8858-8863 JJ denotes total
T5588 8864-8872 NN denotes proteins
T5589 8872-8873 -RRB- denotes )
T5590 8874-8878 VB denotes were
T5591 8879-8887 VB denotes resolved
T5592 8888-8890 IN denotes in
T5593 8891-8892 DT denotes a
T5594 8893-8903 VB denotes denaturing
T5595 8904-8906 CD denotes 12
T5596 8906-8907 NN denotes %
T5597 8908-8922 NN denotes polyacrylamide
T5598 8923-8926 NN denotes gel
T5599 8927-8930 CC denotes and
T5600 8931-8942 VB denotes transferred
T5601 8943-8945 TO denotes to
T5602 8946-8947 DT denotes a
T5603 8948-8962 NN denotes nitrocellulose
T5604 8963-8971 NN denotes membrane
T5605 8973-8978 JJ denotes I-κBα
T5606 8979-8986 NN denotes protein
T5607 8987-8990 VB denotes was
T5608 8991-8999 VB denotes detected
T5609 9000-9005 VB denotes using
T5610 9006-9007 DT denotes a
T5611 9008-9014 NN denotes rabbit
T5612 9015-9025 JJ denotes polyclonal
T5613 9026-9034 NN denotes antibody
T5614 9035-9036 -LRB- denotes (
T5615 9036-9041 NNP denotes Santa
T5616 9042-9046 NNP denotes Cruz
T5617 9047-9060 NNP denotes Biotechnology
T5618 9060-9061 -COMMA- denotes ,
T5619 9062-9064 NN denotes CA
T5620 9064-9065 -RRB- denotes )
T5621 9066-9074 VB denotes followed
T5622 9075-9077 IN denotes by
T5623 9078-9079 DT denotes a
T5624 9080-9091 JJ denotes horseradish
T5625 9092-9110 JJ denotes peroxidase-coupled
T5626 9111-9115 NN denotes goat
T5627 9116-9126 JJ denotes polyclonal
T5628 9127-9135 NN denotes antibody
T5629 9136-9143 IN denotes against
T5630 9144-9150 NN denotes rabbit
T5631 9151-9153 NN denotes Ig
T5632 9154-9155 -LRB- denotes (
T5633 9155-9161 NNP denotes Caltag
T5634 9162-9174 NNP denotes Laboratories
T5635 9174-9175 -RRB- denotes )
T5636 9177-9184 RB denotes Finally
T5637 9184-9185 -COMMA- denotes ,
T5638 9186-9189 NN denotes IκB
T5639 9190-9195 NN denotes bands
T5640 9196-9200 VB denotes were
T5641 9201-9209 VB denotes revealed
T5642 9210-9215 VB denotes using
T5643 9216-9219 DT denotes the
T5644 9220-9224 NN denotes ECL™
T5645 9225-9234 NN denotes detection
T5646 9235-9241 NN denotes system
T5647 9242-9243 -LRB- denotes (
T5648 9243-9251 NNP denotes Amersham
T5649 9252-9261 NNP denotes Pharmacia
T5650 9262-9269 NNP denotes Biotech
T5651 9269-9270 -COMMA- denotes ,
T5652 9271-9274 NNP denotes Les
T5653 9275-9280 NNP denotes Ullis
T5654 9280-9281 -COMMA- denotes ,
T5655 9282-9288 NNP denotes France
T5656 9288-9289 -RRB- denotes )
T5657 9290-9299 VB denotes according
T5658 9300-9302 TO denotes to
T5659 9303-9306 DT denotes the
T5660 9307-9320 NN denotes manufacturers
T5661 9320-9321 POS denotes '
T5662 9322-9333 NN denotes instruction
T5663 9335-9343 NN denotes Antibody
T5664 9344-9346 TO denotes to
T5665 9347-9356 NN denotes α-Tubulin
T5666 9357-9358 -LRB- denotes (
T5667 9358-9363 NNP denotes Santa
T5668 9364-9368 NNP denotes Cruz
T5669 9368-9369 -RRB- denotes )
T5670 9370-9373 VB denotes was
T5671 9374-9377 NN denotes use
T5672 9378-9380 IN denotes as
T5673 9381-9388 VB denotes loading
T5674 9389-9396 NN denotes control
T5675 9398-9401 IN denotes For
T5676 9402-9409 JJ denotes nuclear
T5677 9410-9415 NN denotes NF-κB
T5678 9415-9416 -COMMA- denotes ,
T5679 9417-9422 NN denotes THP-1
T5680 9423-9428 NN denotes cells
T5681 9429-9433 VB denotes were
T5682 9434-9444 VB denotes stimulated
T5683 9445-9449 IN denotes with
T5684 9450-9451 CD denotes 1
T5685 9452-9457 NN denotes mg/ml
T5686 9458-9461 NN denotes C10
T5687 9462-9464 CC denotes or
T5688 9465-9468 NN denotes C40
T5689 9469-9472 IN denotes for
T5690 9473-9475 CD denotes 30
T5691 9476-9483 NN denotes minutes
T5692 9484-9486 IN denotes at
T5693 9487-9492 NNP denotes 37°C.
T5694 9493-9498 NNP denotes Cells
T5695 9499-9503 VB denotes were
T5696 9504-9508 RB denotes then
T5697 9509-9517 VB denotes pelleted
T5698 9518-9521 CC denotes and
T5699 9522-9528 NN denotes nuclei
T5700 9529-9538 VB denotes separated
T5701 9539-9541 IN denotes as
T5702 9542-9551 VB denotes described
T5703 9552-9553 -LRB- denotes [
T5704 9553-9555 CD denotes 31
T5705 9555-9556 -RRB- denotes ]
T5706 9558-9564 NN denotes Nuclei
T5707 9565-9569 VB denotes were
T5708 9570-9576 VB denotes washed
T5709 9577-9580 CC denotes and
T5710 9581-9592 VB denotes homogenized
T5711 9593-9601 RB denotes directly
T5712 9602-9604 IN denotes in
T5713 9605-9612 VB denotes loading
T5714 9613-9614 -LRB- denotes (
T5715 9614-9620 NNP denotes Laemli
T5716 9620-9621 -RRB- denotes )
T5717 9622-9628 NN denotes buffer
T5718 9629-9632 CC denotes and
T5719 9633-9639 VB denotes heated
T5720 9640-9643 IN denotes for
T5721 9644-9645 CD denotes 5
T5722 9646-9653 NN denotes minutes
T5723 9654-9656 IN denotes at
T5724 9657-9663 NNP denotes 100°C.
T5725 9664-9672 NNP denotes Proteins
T5726 9673-9675 IN denotes in
T5727 9676-9683 NN denotes samples
T5728 9684-9688 VB denotes were
T5729 9689-9697 VB denotes resolved
T5730 9698-9700 IN denotes in
T5731 9701-9702 DT denotes a
T5732 9703-9713 VB denotes denaturing
T5733 9714-9715 CD denotes 8
T5734 9715-9716 NN denotes %
T5735 9717-9731 NN denotes polyacrylamide
T5736 9732-9735 NN denotes gel
T5737 9736-9739 CC denotes and
T5738 9740-9751 VB denotes transferred
T5739 9752-9754 TO denotes to
T5740 9755-9756 DT denotes a
T5741 9757-9771 NN denotes polyvinylidine
T5742 9772-9780 NN denotes fluoride
T5743 9781-9782 -LRB- denotes (
T5744 9782-9786 NN denotes PVDF
T5745 9786-9787 -RRB- denotes )
T5746 9788-9796 NN denotes membrane
T5747 9797-9798 -LRB- denotes (
T5748 9798-9809 NN denotes Immobilon-P
T5749 9809-9810 -COLON- denotes ;
T5750 9811-9820 NNP denotes Millipore
T5751 9820-9821 -COMMA- denotes ,
T5752 9822-9829 NNP denotes Bedford
T5753 9829-9830 -COMMA- denotes ,
T5754 9831-9833 JJ denotes MA
T5755 9833-9834 -RRB- denotes )
T5756 9836-9845 NN denotes Membranes
T5757 9846-9850 VB denotes were
T5758 9851-9860 VB denotes incubated
T5759 9861-9863 IN denotes in
T5760 9864-9872 VB denotes blocking
T5761 9873-9879 NN denotes buffer
T5762 9880-9881 -LRB- denotes (
T5763 9881-9882 CD denotes 1
T5764 9882-9883 NN denotes %
T5765 9884-9887 NN denotes BSA
T5766 9887-9888 -COMMA- denotes ,
T5767 9889-9891 IN denotes in
T5768 9892-9895 NNP denotes PBS
T5769 9895-9896 -RRB- denotes )
T5770 9897-9900 IN denotes for
T5771 9901-9904 CD denotes two
T5772 9905-9910 NN denotes hours
T5773 9911-9913 IN denotes at
T5774 9914-9918 NN denotes room
T5775 9919-9930 NN denotes temperature
T5776 9932-9941 NN denotes Membranes
T5777 9942-9946 VB denotes were
T5778 9947-9959 RB denotes subsequently
T5779 9960-9966 VB denotes probed
T5780 9967-9971 IN denotes with
T5781 9972-9975 DT denotes the
T5782 9976-9989 JJ denotes corresponding
T5783 9990-9998 NN denotes antibody
T5784 9999-10001 IN denotes in
T5785 10002-10010 VB denotes blocking
T5786 10011-10017 NN denotes buffer
T5787 10017-10018 -COMMA- denotes ,
T5788 10019-10028 RB denotes overnight
T5789 10030-10036 NN denotes Rabbit
T5790 10037-10047 JJ denotes polyclonal
T5791 10048-10056 NN denotes antibody
T5792 10057-10067 JJ denotes anti-NF-κB
T5793 10068-10071 NN denotes p50
T5794 10072-10079 NN denotes subunit
T5795 10080-10081 -LRB- denotes (
T5796 10081-10082 -SHARP- denotes #
T5797 10083-10089 NN denotes sc-114
T5798 10089-10090 -RRB- denotes )
T5799 10091-10093 CC denotes or
T5800 10094-10104 JJ denotes anti-NF-κB
T5801 10105-10108 NN denotes p65
T5802 10109-10116 NN denotes subunit
T5803 10117-10118 -LRB- denotes (
T5804 10118-10119 -SHARP- denotes #
T5805 10120-10126 NN denotes sc-109
T5806 10126-10127 -RRB- denotes )
T5807 10128-10132 IN denotes from
T5808 10133-10138 NNP denotes Santa
T5809 10139-10143 NNP denotes Cruz
T5810 10144-10157 NNP denotes Biotechnology
T5811 10158-10162 VB denotes were
T5812 10163-10167 VB denotes used
T5813 10169-10178 NN denotes Membranes
T5814 10179-10183 VB denotes were
T5815 10184-10190 VB denotes washed
T5816 10191-10194 CD denotes six
T5817 10195-10200 NN denotes times
T5818 10201-10203 IN denotes in
T5819 10204-10207 NNP denotes PBS
T5820 10208-10212 IN denotes with
T5821 10213-10217 CD denotes 0.05
T5822 10217-10218 NN denotes %
T5823 10219-10224 CD denotes Tween
T5824 10225-10227 CD denotes 20
T5825 10227-10228 -COMMA- denotes ,
T5826 10229-10230 CD denotes 5
T5827 10231-10238 NN denotes minutes
T5828 10239-10243 DT denotes each
T5829 10244-10248 NN denotes time
T5830 10248-10249 -COMMA- denotes ,
T5831 10250-10253 CC denotes and
T5832 10254-10263 VB denotes incubated
T5833 10264-10268 IN denotes with
T5834 10269-10270 DT denotes a
T5835 10271-10277 CD denotes 1/3000
T5836 10278-10286 NN denotes dilution
T5837 10287-10289 IN denotes of
T5838 10290-10304 JJ denotes HRP-conjugated
T5839 10305-10306 NN denotes F
T5840 10306-10307 -LRB- denotes (
T5841 10307-10309 NN denotes ab
T5842 10309-10310 -DQE- denotes '
T5843 10310-10311 -RRB- denotes )
T5844 10311-10312 CD denotes 2
T5845 10313-10317 NN denotes goat
T5846 10318-10329 JJ denotes anti-rabbit
T5847 10330-10333 NN denotes IgG
T5848 10334-10336 IN denotes in
T5849 10337-10338 CD denotes 5
T5850 10338-10339 NN denotes %
T5851 10340-10346 JJ denotes nonfat
T5852 10347-10350 JJ denotes dry
T5853 10351-10355 NN denotes milk
T5854 10356-10359 CC denotes and
T5855 10360-10364 CD denotes 0.05
T5856 10364-10365 NN denotes %
T5857 10366-10371 CD denotes Tween
T5858 10372-10374 CD denotes 20
T5859 10375-10377 IN denotes in
T5860 10378-10381 NNP denotes PBS
T5861 10382-10385 IN denotes for
T5862 10386-10387 CD denotes 1
T5863 10388-10392 NN denotes hour
T5864 10393-10395 IN denotes at
T5865 10396-10400 NN denotes room
T5866 10401-10412 NN denotes temperature
T5867 10414-10419 IN denotes After
T5868 10420-10427 VB denotes washing
T5869 10428-10431 CD denotes six
T5870 10432-10436 JJ denotes more
T5871 10437-10442 NN denotes times
T5872 10443-10445 IN denotes in
T5873 10446-10449 NNP denotes PBS
T5874 10450-10454 IN denotes with
T5875 10455-10459 CD denotes 0.05
T5876 10459-10460 NN denotes %
T5877 10461-10466 CD denotes Tween
T5878 10467-10469 CD denotes 20
T5879 10469-10470 -COMMA- denotes ,
T5880 10471-10488 JJ denotes antibody-reactive
T5881 10489-10497 NN denotes proteins
T5882 10498-10502 VB denotes were
T5883 10503-10511 VB denotes detected
T5884 10512-10517 VB denotes using
T5885 10518-10519 DT denotes a
T5886 10520-10537 NN denotes chemiluminescence
T5887 10538-10547 NN denotes substrate
T5888 10548-10549 -LRB- denotes (
T5889 10549-10560 NNP denotes SuperSignal
T5890 10560-10561 -COLON- denotes ;
T5891 10562-10568 NNP denotes Pierce
T5892 10568-10569 -COMMA- denotes ,
T5893 10570-10578 NNP denotes Rockford
T5894 10578-10579 -COMMA- denotes ,
T5895 10580-10582 NN denotes IL
T5896 10582-10583 -RRB- denotes )
T5897 10584-10593 VB denotes according
T5898 10594-10596 TO denotes to
T5899 10597-10600 DT denotes the
T5900 10601-10613 NN denotes manufacturer
T5901 10613-10615 POS denotes 's
T5902 10616-10628 NN denotes instructions
T5903 10630-10632 TO denotes To
T5904 10633-10640 VB denotes confirm
T5905 10641-10645 IN denotes that
T5906 10646-10656 JJ denotes equivalent
T5907 10657-10664 NN denotes amounts
T5908 10665-10667 IN denotes of
T5909 10668-10675 NN denotes protein
T5910 10676-10680 VB denotes were
T5911 10681-10687 VB denotes loaded
T5912 10688-10690 IN denotes in
T5913 10691-10695 DT denotes each
T5914 10696-10700 NN denotes line
T5915 10700-10701 -COMMA- denotes ,
T5916 10702-10711 NN denotes membranes
T5917 10712-10716 VB denotes were
T5918 10717-10721 RB denotes also
T5919 10722-10729 JJ denotes Western
T5920 10730-10737 VB denotes blotted
T5921 10738-10741 IN denotes for
T5922 10742-10745 NN denotes ERK
T5923 10746-10748 IN denotes as
T5924 10749-10758 VB denotes described
T5925 10759-10760 -LRB- denotes [
T5926 10760-10762 CD denotes 32
T5927 10762-10763 -RRB- denotes ]
T6465 10766-10774 NN denotes Analysis
T6466 10775-10777 IN denotes of
T6467 10778-10783 NN denotes NF-κB
T6468 10784-10794 NN denotes Activation
T6469 10795-10797 IN denotes by
T6470 10798-10802 NN denotes Flow
T6471 10803-10812 NN denotes Cytometry
T6472 10813-10820 JJ denotes Nuclear
T6473 10821-10831 NN denotes activation
T6474 10832-10834 IN denotes of
T6475 10835-10840 NN denotes NF−κΒ
T6476 10841-10843 IN denotes by
T6477 10844-10848 NN denotes flow
T6478 10849-10858 NN denotes cytometry
T6479 10859-10862 VB denotes was
T6480 10863-10872 VB denotes performed
T6481 10873-10875 IN denotes as
T6482 10876-10885 VB denotes described
T6483 10886-10887 -LRB- denotes [
T6484 10887-10889 CD denotes 31
T6485 10889-10890 -RRB- denotes ]
T6520 10893-10904 JJ denotes Statistical
T6521 10905-10913 NN denotes Analysis
T6522 10914-10917 DT denotes The
T6523 10918-10925 NN denotes results
T6524 10926-10930 VB denotes were
T6525 10931-10940 VB denotes expressed
T6526 10941-10943 IN denotes as
T6527 10944-10947 DT denotes the
T6528 10948-10952 JJ denotes mean
T6529 10953-10958 NN denotes value
T6530 10959-10960 VB denotes ±
T6531 10961-10967 NNP denotes S.E.M.
T6532 10968-10970 IN denotes of
T6533 10971-10981 JJ denotes individual
T6534 10982-10993 NN denotes experiments
T6535 10995-10998 DT denotes The
T6536 10999-11010 JJ denotes statistical
T6537 11011-11023 NN denotes significance
T6538 11024-11026 IN denotes of
T6539 11027-11030 DT denotes the
T6540 11031-11042 NN denotes differences
T6541 11043-11050 IN denotes between
T6542 11051-11055 JJ denotes mean
T6543 11056-11062 NN denotes values
T6544 11063-11066 VB denotes was
T6545 11067-11075 VB denotes assessed
T6546 11076-11078 IN denotes by
T6547 11079-11082 DT denotes the
T6548 11083-11090 NN denotes Student
T6549 11090-11092 POS denotes 's
T6550 11093-11099 NN denotes t-test
T6551 11100-11103 CC denotes and
T6552 11104-11112 NN denotes analysis
T6553 11113-11115 IN denotes of
T6554 11116-11124 NN denotes variance
T6555 11125-11126 -LRB- denotes (
T6556 11126-11131 NN denotes ANOVA
T6557 11131-11132 -RRB- denotes )
T2817 2021-2028 NN denotes Animals
R1776 T2063 T2064 arg1Of Preparation,of
R1777 T2066 T2064 arg2Of Carrageenan,of
R1778 T2066 T2065 arg1Of Carrageenan,Degraded
R1779 T2068 T2067 arg1Of preparations,Two
R1780 T2068 T2069 arg1Of preparations,of
R1781 T2068 T2093 modOf preparations,were
R1784 T2071 T2069 arg2Of carrageenan,of
R1786 T2073 T2075 arg1Of low,(
R1787 T2073 T2082 arg1Of low,and
R1788 T2077 T2075 arg2Of kDa,(
R1789 T2077 T2076 arg1Of kDa,∼10
R1790 T2077 T2078 arg1Of kDa,;
R1791 T2079 T2078 arg2Of C10,;
R1792 T2080 T2075 arg3Of ),(
R1794 T2082 T2081 arg1Of and,","
R1795 T2083 T2082 arg2Of medium,and
R1796 T2087 T2085 arg2Of kDa,(
R1797 T2087 T2086 arg1Of kDa,∼40
R1798 T2087 T2088 arg1Of kDa,;
R1799 T2089 T2088 arg2Of C40,;
R1800 T2090 T2085 arg3Of ),(
R1801 T2092 T2085 arg1Of weight,(
R1802 T2092 T2091 arg1Of weight,molecular
R1803 T2092 T2093 arg1Of weight,were
R1804 T2092 T2094 arg2Of weight,prepared
R1805 T2094 T2084 arg1Of prepared,","
R1806 T2094 T2093 arg2Of prepared,were
R1807 T2094 T2095 arg1Of prepared,from
R1808 T2097 T2095 arg2Of iota-carrageenan,from
R1809 T2097 T2096 arg1Of iota-carrageenan,native
R1810 T2097 T2098 arg2Of iota-carrageenan,extracted
R1811 T2098 T2099 arg1Of extracted,from
R1812 T2101 T2099 arg2Of spinosum,from
R1813 T2101 T2100 arg1Of spinosum,Euchema
R1814 T2101 T2102 arg1Of spinosum,(
R1815 T2104 T2102 arg2Of provided,(
R1816 T2104 T2103 arg1Of provided,generously
R1817 T2108 T2104 arg1Of Industry,provided
R1818 T2108 T2105 arg2Of Industry,by
R1819 T2108 T2106 arg1Of Industry,Sanofi
R1820 T2108 T2107 arg1Of Industry,Biosystems
R1821 T2108 T2109 arg1Of Industry,","
R1822 T2110 T2109 arg2Of Boulogne-Billancourt,","
R1823 T2110 T2111 arg1Of Boulogne-Billancourt,","
R1824 T2112 T2111 arg2Of France,","
R1825 T2113 T2102 arg3Of ),(
R1826 T2115 T2114 arg1Of carrageenan,Native
R1827 T2115 T2116 arg1Of carrageenan,was
R1828 T2115 T2117 arg2Of carrageenan,dissolved
R1829 T2115 T2130 arg2Of carrageenan,heated
R1830 T2117 T2118 arg1Of dissolved,in
R1831 T2117 T2129 arg1Of dissolved,and
R1832 T2120 T2118 arg2Of water,in
R1833 T2120 T2119 arg1Of water,distilled
R1834 T2120 T2121 arg1Of water,(
R1835 T2120 T2126 arg1Of water,under
R1836 T2122 T2123 arg1Of 5,%
R1837 T2124 T2121 arg2Of w/v,(
R1838 T2124 T2122 arg1Of w/v,5
R1839 T2125 T2121 arg3Of ),(
R1840 T2128 T2126 arg2Of stirring,under
R1841 T2128 T2127 arg1Of stirring,vigorous
R1842 T2129 T2116 arg2Of and,was
R1843 T2130 T2129 arg2Of heated,and
R1844 T2130 T2131 arg1Of heated,to
R1845 T2133 T2131 arg2Of Then,to
R1846 T2133 T2132 arg1Of Then,60°C.
R1847 T2137 T2135 arg1Of solution,the
R1848 T2137 T2136 arg1Of solution,carrageenan
R1849 T2137 T2138 arg1Of solution,was
R1850 T2137 T2139 arg2Of solution,submitted
R1851 T2139 T2116 modOf submitted,was
R1852 T2139 T2134 arg1Of submitted,","
R1853 T2139 T2138 arg2Of submitted,was
R1854 T2139 T2140 arg1Of submitted,to
R1855 T2143 T2140 arg2Of treatments,to
R1856 T2143 T2141 arg1Of treatments,two
R1857 T2143 T2142 arg1Of treatments,different
R1858 T2145 T2139 arg3Of obtain,submitted
R1859 T2145 T2144 arg1Of obtain,to
R1860 T2147 T2148 arg1Of low,and
R1861 T2148 T2146 arg1Of and,both
R1862 T2149 T2148 arg2Of medium,and
R1863 T2152 T2145 arg2Of fractions,obtain
R1864 T2152 T2147 arg1Of fractions,low
R1865 T2152 T2149 arg1Of fractions,medium
R1866 T2152 T2150 arg1Of fractions,molecular
R1867 T2152 T2151 arg1Of fractions,weight
R1868 T2160 T2155 arg2Of fraction,for
R1869 T2160 T2156 arg1Of fraction,the
R1870 T2160 T2157 arg1Of fraction,low
R1871 T2160 T2158 arg1Of fraction,molecular
R1872 T2160 T2159 arg1Of fraction,weight
R1873 T2163 T2162 arg1Of solution,carrageenan
R1874 T2163 T2164 arg1Of solution,was
R1875 T2163 T2165 arg2Of solution,hydrolyzed
R1876 T2165 T2164 arg2Of hydrolyzed,was
R1877 T2165 T2166 arg1Of hydrolyzed,with
R1878 T2168 T2166 arg2Of %,with
R1879 T2168 T2167 arg1Of %,0.3
R1880 T2168 T2169 arg1Of %,(
R1881 T2168 T2172 arg2Of %,concentrated
R1882 T2170 T2169 arg2Of v/v,(
R1883 T2171 T2169 arg3Of ),(
R1884 T2174 T2172 arg1Of acid,concentrated
R1885 T2174 T2173 arg1Of acid,sulphuric
R1886 T2174 T2175 arg1Of acid,for
R1887 T2174 T2178 arg1Of acid,at
R1888 T2177 T2175 arg2Of min,for
R1889 T2177 T2176 arg1Of min,15
R1890 T2180 T2179 arg1Of After,80°C.
R1891 T2181 T2178 arg2Of neutralization,at
R1892 T2181 T2180 arg1Of neutralization,After
R1893 T2181 T2182 arg1Of neutralization,with
R1894 T2184 T2182 arg2Of 4N,with
R1895 T2184 T2183 arg1Of 4N,NaOH
R1896 T2187 T2186 arg1Of solution,the
R1897 T2187 T2188 arg1Of solution,was
R1898 T2188 T2153 arg1Of was,Briefly
R1899 T2188 T2154 arg1Of was,","
R1900 T2188 T2155 arg1Of was,for
R1901 T2188 T2161 arg1Of was,","
R1902 T2188 T2164 modOf was,was
R1903 T2188 T2185 arg1Of was,","
R1904 T2189 T2188 arg2Of ultra,was
R1905 T2189 T2190 arg2Of ultra,filtered
R1906 T2189 T2201 arg1Of ultra,","
R1907 T2189 T2202 arg1Of ultra,(
R1908 T2190 T2191 arg1Of filtered,through
R1909 T2195 T2191 arg2Of cartridge,through
R1910 T2195 T2192 arg1Of cartridge,a
R1911 T2195 T2193 arg1Of cartridge,hollow
R1912 T2195 T2194 arg1Of cartridge,fibre
R1913 T2195 T2196 arg1Of cartridge,with
R1914 T2200 T2196 arg2Of kDa,with
R1915 T2200 T2197 arg1Of kDa,MW
R1916 T2200 T2198 arg1Of kDa,cut-off
R1917 T2200 T2199 arg1Of kDa,5
R1918 T2204 T2202 arg2Of Inc,(
R1919 T2204 T2203 arg1Of Inc,Amicon
R1920 T2204 T2205 arg1Of Inc,","
R1921 T2206 T2205 arg2Of Beverly,","
R1922 T2206 T2207 arg1Of Beverly,","
R1923 T2208 T2207 arg2Of USA,","
R1924 T2209 T2202 arg3Of ),(
R1925 T2215 T2211 arg1Of fraction,the
R1926 T2215 T2212 arg1Of fraction,medium
R1927 T2215 T2213 arg1Of fraction,molecular
R1928 T2215 T2214 arg1Of fraction,weight
R1929 T2215 T2228 arg1Of fraction,concentrated
R1930 T2219 T2217 arg1Of solution,the
R1931 T2219 T2218 arg1Of solution,carrageenan
R1932 T2219 T2220 arg1Of solution,was
R1933 T2219 T2221 arg2Of solution,hydrolyzed
R1934 T2221 T2220 arg2Of hydrolyzed,was
R1935 T2221 T2222 arg1Of hydrolyzed,with
R1936 T2224 T2222 arg2Of %,with
R1937 T2224 T2223 arg1Of %,0.3
R1938 T2224 T2225 arg1Of %,(
R1939 T2226 T2225 arg2Of v/v,(
R1940 T2227 T2225 arg3Of ),(
R1941 T2228 T2210 arg1Of concentrated,For
R1942 T2228 T2216 arg1Of concentrated,","
R1943 T2228 T2220 modOf concentrated,was
R1944 T2228 T2244 arg1Of concentrated,(
R1945 T2230 T2228 arg2Of acid,concentrated
R1946 T2230 T2229 arg1Of acid,sulphuric
R1947 T2230 T2231 arg1Of acid,for
R1948 T2230 T2234 arg1Of acid,at
R1949 T2230 T2243 arg2Of acid,filtered
R1950 T2233 T2231 arg2Of min,for
R1951 T2233 T2232 arg1Of min,30
R1952 T2235 T2234 arg2Of 60°C.,at
R1953 T2235 T2236 arg1Of 60°C.,After
R1954 T2237 T2236 arg2Of neutralization,After
R1955 T2240 T2239 arg1Of supernatant,the
R1956 T2240 T2241 arg1Of supernatant,was
R1957 T2240 T2242 arg1Of supernatant,ultra
R1958 T2241 T2238 arg1Of was,","
R1959 T2242 T2241 arg2Of ultra,was
R1960 T2243 T2241 arg3Of filtered,was
R1961 T2245 T2244 arg2Of MW,(
R1962 T2245 T2246 arg1Of MW,cut-off
R1963 T2246 T2248 arg1Of cut-off,kDa
R1964 T2248 T2247 arg1Of kDa,100
R1965 T2249 T2244 arg3Of ),(
R1966 T2251 T2250 arg1Of filtrate,The
R1967 T2251 T2252 arg1Of filtrate,was
R1968 T2251 T2253 arg2Of filtrate,submitted
R1969 T2253 T2252 arg2Of submitted,was
R1970 T2253 T2254 arg1Of submitted,to
R1971 T2258 T2254 arg2Of filtration,to
R1972 T2258 T2255 arg1Of filtration,a
R1973 T2258 T2256 arg1Of filtration,second
R1974 T2258 T2257 arg1Of filtration,ultra
R1975 T2258 T2259 arg1Of filtration,(
R1976 T2263 T2259 arg2Of kDa,(
R1977 T2263 T2260 arg1Of kDa,MW
R1978 T2263 T2261 arg1Of kDa,cut-off
R1979 T2263 T2262 arg1Of kDa,5
R1980 T2264 T2259 arg3Of ),(
R1981 T2266 T2265 arg1Of preparations,Both
R1982 T2266 T2267 arg1Of preparations,of
R1983 T2266 T2269 arg1Of preparations,were
R1984 T2266 T2270 arg2Of preparations,precipitated
R1985 T2268 T2267 arg2Of dCGN,of
R1986 T2270 T2269 arg2Of precipitated,were
R1987 T2270 T2271 arg1Of precipitated,with
R1988 T2273 T2271 arg2Of volumes,with
R1989 T2273 T2272 arg1Of volumes,4
R1990 T2273 T2274 arg1Of volumes,of
R1991 T2273 T2279 arg2Of volumes,dried
R1992 T2275 T2276 arg1Of 95,%
R1993 T2277 T2274 arg2Of ethanol,of
R1994 T2277 T2275 arg1Of ethanol,95
R1995 T2279 T2278 arg1Of dried,","
R1996 T2279 T2280 arg1Of dried,at
R1997 T2282 T2281 arg1Of temperature,room
R1998 T2282 T2283 arg1Of temperature,and
R1999 T2283 T2280 arg2Of and,at
R2000 T2284 T2283 arg2Of ground,and
R2001 T2284 T2285 arg1Of ground,to
R2002 T2287 T2285 arg2Of particles,to
R2003 T2287 T2286 arg1Of particles,small
R2004 T2287 T2288 arg1Of particles,(
R2005 T2290 T2288 arg2Of mm,(
R2006 T2290 T2289 arg1Of mm,1
R2007 T2290 T2291 arg1Of mm,in
R2008 T2292 T2291 arg2Of diameter,in
R2009 T2293 T2288 arg3Of ),(
R2010 T2296 T2294 arg1Of chromatography,Using
R2011 T2296 T2295 arg1Of chromatography,gel-permeation
R2012 T2296 T2297 arg1Of chromatography,in
R2013 T2296 T2299 arg1Of chromatography,with
R2014 T2296 T2304 arg1Of chromatography,see
R2015 T2298 T2297 arg2Of combination,in
R2016 T2302 T2299 arg2Of measurements,with
R2017 T2302 T2300 arg1Of measurements,light
R2018 T2302 T2301 arg1Of measurements,scattering
R2019 T2304 T2303 arg1Of see,(
R2020 T2307 T2305 arg1Of al.,Viebke
R2021 T2307 T2306 arg1Of al.,et
R2022 T2307 T2308 arg1Of al.,[
R2023 T2307 T2311 arg1Of al.,)
R2024 T2307 T2312 arg1Of al.,","
R2025 T2307 T2314 arg1Of al.,was
R2026 T2307 T2315 arg2Of al.,confirmed
R2027 T2309 T2308 arg2Of 21,[
R2028 T2310 T2308 arg3Of ],[
R2029 T2313 T2312 arg2Of it,","
R2030 T2315 T2314 arg2Of confirmed,was
R2031 T2316 T2315 arg3Of that,confirmed
R2032 T2319 T2317 arg1Of fraction,the
R2033 T2319 T2318 arg1Of fraction,low
R2034 T2319 T2320 arg1Of fraction,had
R2035 T2320 T2304 arg2Of had,see
R2036 T2320 T2314 modOf had,was
R2037 T2324 T2321 arg1Of weight,an
R2038 T2324 T2322 arg1Of weight,average
R2039 T2324 T2323 arg1Of weight,molecular
R2040 T2324 T2325 arg1Of weight,of
R2041 T2324 T2329 arg1Of weight,and
R2042 T2327 T2325 arg2Of kDa,of
R2043 T2327 T2326 arg1Of kDa,10
R2044 T2329 T2320 arg2Of and,had
R2045 T2329 T2328 arg1Of and,","
R2046 T2332 T2329 arg2Of fraction,and
R2047 T2332 T2330 arg1Of fraction,the
R2048 T2332 T2331 arg1Of fraction,medium
R2049 T2332 T2333 arg1Of fraction,of
R2050 T2335 T2333 arg2Of kDa,of
R2051 T2335 T2334 arg1Of kDa,40
R2052 T2338 T2336 arg1Of content,The
R2053 T2338 T2337 arg1Of content,sulphate
R2054 T2338 T2339 arg1Of content,of
R2055 T2338 T2341 arg1Of content,in
R2056 T2338 T2344 arg1Of content,was
R2057 T2338 T2345 arg2Of content,measured
R2058 T2340 T2339 arg2Of polysaccharides,of
R2059 T2343 T2341 arg2Of fractions,in
R2060 T2343 T2342 arg1Of fractions,both
R2061 T2345 T2344 arg2Of measured,was
R2062 T2345 T2346 arg1Of measured,following
R2063 T2348 T2346 arg2Of method,following
R2064 T2348 T2347 arg1Of method,the
R2065 T2348 T2349 arg1Of method,of
R2066 T2352 T2349 arg2Of al.,of
R2067 T2352 T2350 arg1Of al.,Quemener
R2068 T2352 T2351 arg1Of al.,et
R2069 T2352 T2353 arg1Of al.,[
R2070 T2354 T2353 arg2Of 22,[
R2071 T2355 T2353 arg3Of ],[
R2072 T2359 T2358 arg1Of absence,the
R2073 T2359 T2360 arg1Of absence,of
R2074 T2359 T2368 arg1Of absence,was
R2075 T2359 T2369 arg2Of absence,confirmed
R2076 T2363 T2360 arg2Of modifications,of
R2077 T2363 T2361 arg1Of modifications,polysaccharide
R2078 T2363 T2362 arg1Of modifications,structure
R2079 T2363 T2364 arg1Of modifications,in
R2080 T2367 T2364 arg2Of fractions,in
R2081 T2367 T2365 arg1Of fractions,the
R2082 T2367 T2366 arg1Of fractions,two
R2083 T2369 T2356 arg1Of confirmed,Finally
R2084 T2369 T2357 arg1Of confirmed,","
R2085 T2369 T2368 arg2Of confirmed,was
R2086 T2370 T2369 arg3Of using,confirmed
R2087 T2372 T2370 arg2Of spectroscopy,using
R2088 T2372 T2371 arg1Of spectroscopy,2H-NMR
R2089 T2374 T2373 arg1Of absence,The
R2090 T2374 T2375 arg1Of absence,of
R2091 T2374 T2378 arg1Of absence,in
R2092 T2374 T2382 arg1Of absence,was
R2093 T2374 T2383 arg2Of absence,confirmed
R2094 T2377 T2375 arg2Of contamination,of
R2095 T2377 T2376 arg1Of contamination,LPS
R2096 T2381 T2378 arg2Of fractions,in
R2097 T2381 T2379 arg1Of fractions,the
R2098 T2381 T2380 arg1Of fractions,two
R2099 T2383 T2382 arg2Of confirmed,was
R2100 T2384 T2383 arg3Of using,confirmed
R2101 T2384 T2388 arg1Of using,(
R2102 T2387 T2384 arg2Of kit,using
R2103 T2387 T2385 arg1Of kit,the
R2104 T2387 T2386 arg1Of kit,e-Toxate®
R2105 T2389 T2388 arg2Of Sigma,(
R2106 T2389 T2390 arg1Of Sigma,","
R2107 T2393 T2390 arg2Of Fallavier,","
R2108 T2393 T2391 arg1Of Fallavier,St
R2109 T2393 T2392 arg1Of Fallavier,Quentin
R2110 T2393 T2394 arg1Of Fallavier,","
R2111 T2395 T2394 arg2Of France,","
R2112 T2396 T2388 arg3Of ),(
R2113 T2398 T2397 arg2Of use,Before
R2114 T2398 T2399 arg1Of use,in
R2115 T2401 T2399 arg2Of culture,in
R2116 T2401 T2400 arg1Of culture,cell
R2117 T2405 T2403 arg1Of fractions,the
R2118 T2405 T2404 arg1Of fractions,two
R2119 T2405 T2406 arg1Of fractions,were
R2120 T2405 T2407 arg2Of fractions,dissolved
R2121 T2407 T2397 arg1Of dissolved,Before
R2122 T2407 T2402 arg1Of dissolved,","
R2123 T2407 T2406 arg2Of dissolved,were
R2124 T2407 T2408 arg1Of dissolved,in
R2125 T2407 T2411 arg1Of dissolved,during
R2126 T2410 T2408 arg2Of medium,in
R2127 T2410 T2409 arg1Of medium,complete
R2128 T2413 T2411 arg2Of min,during
R2129 T2413 T2412 arg1Of min,30
R2130 T2413 T2414 arg1Of min,at
R2131 T2415 T2414 arg2Of 56°C,at
R2508 T2817 T2818 arg1Of Animals,","
R2509 T2818 T2820 arg1Of ",",and
R2510 T2819 T2818 arg2Of Chemicals,","
R2511 T2821 T2820 arg2Of Diet,and
R2512 T2824 T2822 arg1Of rats,Male
R2513 T2824 T2823 arg1Of rats,Wistar
R2514 T2824 T2825 arg1Of rats,(
R2515 T2824 T2831 arg1Of rats,were
R2516 T2824 T2832 arg2Of rats,housed
R2517 T2829 T2825 arg2Of weight,(
R2518 T2829 T2826 arg1Of weight,150
R2519 T2829 T2827 arg1Of weight,g
R2520 T2829 T2828 arg1Of weight,average
R2521 T2830 T2825 arg3Of ),(
R2522 T2832 T2831 arg2Of housed,were
R2523 T2832 T2833 arg1Of housed,under
R2524 T2835 T2834 arg1Of conditions,standard
R2525 T2835 T2836 arg1Of conditions,and
R2526 T2836 T2833 arg2Of and,under
R2527 T2836 T2840 arg1Of and,with
R2528 T2839 T2836 arg2Of libitum,and
R2529 T2839 T2837 arg2Of libitum,fed
R2530 T2839 T2838 arg1Of libitum,ad
R2531 T2844 T2840 arg2Of chow,with
R2532 T2844 T2841 arg1Of chow,standard
R2533 T2844 T2842 arg1Of chow,rodent
R2534 T2844 T2843 arg1Of chow,laboratory
R2535 T2846 T2845 arg2Of iota-carrageenans,Degraded
R2536 T2846 T2847 arg1Of iota-carrageenans,were
R2537 T2846 T2848 arg2Of iota-carrageenans,administered
R2538 T2848 T2847 arg2Of administered,were
R2539 T2848 T2849 arg1Of administered,in
R2540 T2848 T2858 arg1Of administered,for
R2541 T2852 T2849 arg2Of water,in
R2542 T2852 T2850 arg1Of water,the
R2543 T2852 T2851 arg1Of water,drinking
R2544 T2852 T2853 arg1Of water,(
R2545 T2854 T2855 arg1Of 5,%
R2546 T2856 T2853 arg2Of w/v,(
R2547 T2856 T2854 arg1Of w/v,5
R2548 T2857 T2853 arg3Of ),(
R2549 T2860 T2858 arg2Of days,for
R2550 T2860 T2859 arg1Of days,55
R2551 T2860 T2861 arg1Of days,to
R2552 T2863 T2861 arg2Of groups,to
R2553 T2863 T2862 arg1Of groups,2
R2554 T2863 T2864 arg1Of groups,of
R2555 T2866 T2864 arg2Of animals,of
R2556 T2866 T2865 arg1Of animals,six
R2557 T2866 T2867 arg1Of animals,each
R2558 T2870 T2868 arg1Of group,The
R2559 T2870 T2869 arg1Of group,first
R2560 T2870 T2871 arg1Of group,received
R2561 T2871 T2882 arg1Of received,and
R2562 T2876 T2871 arg2Of carrageenan,received
R2563 T2876 T2872 arg1Of carrageenan,the
R2564 T2876 T2873 arg1Of carrageenan,low
R2565 T2876 T2874 arg1Of carrageenan,molecular
R2566 T2876 T2875 arg1Of carrageenan,weight
R2567 T2876 T2877 arg1Of carrageenan,(
R2568 T2880 T2877 arg2Of dCGN,(
R2569 T2880 T2878 arg1Of dCGN,10
R2570 T2880 T2879 arg1Of dCGN,kDa
R2571 T2881 T2877 arg3Of ),(
R2572 T2884 T2883 arg1Of second,the
R2573 T2884 T2885 arg1Of second,received
R2574 T2885 T2882 arg2Of received,and
R2575 T2890 T2885 arg2Of carrageenan,received
R2576 T2890 T2886 arg1Of carrageenan,the
R2577 T2890 T2887 arg1Of carrageenan,medium
R2578 T2890 T2888 arg1Of carrageenan,molecular
R2579 T2890 T2889 arg1Of carrageenan,weight
R2580 T2890 T2891 arg1Of carrageenan,(
R2581 T2894 T2891 arg2Of dCGN,(
R2582 T2894 T2892 arg1Of dCGN,40
R2583 T2894 T2893 arg1Of dCGN,kDa
R2584 T2895 T2891 arg3Of ),(
R2585 T2898 T2896 arg1Of group,An
R2586 T2898 T2897 arg1Of group,additional
R2587 T2898 T2899 arg1Of group,of
R2588 T2898 T2902 arg1Of group,were
R2589 T2898 T2903 arg2Of group,maintained
R2590 T2901 T2899 arg2Of rats,of
R2591 T2901 T2900 arg1Of rats,four
R2592 T2903 T2902 arg2Of maintained,were
R2593 T2903 T2904 arg1Of maintained,on
R2594 T2907 T2904 arg2Of water,on
R2595 T2907 T2905 arg1Of water,regular
R2596 T2907 T2906 arg1Of water,tap
R2597 T2907 T2908 arg1Of water,(
R2598 T2910 T2908 arg2Of group,(
R2599 T2910 T2909 arg1Of group,control
R2600 T2911 T2908 arg3Of ),(
R2601 T2913 T2912 arg1Of increase,To
R2602 T2913 T2918 arg1Of increase,was
R2603 T2913 T2919 arg2Of increase,added
R2604 T2915 T2916 arg1Of 0.2,%
R2605 T2917 T2913 arg2Of sucrose,increase
R2606 T2917 T2914 arg1Of sucrose,palatability
R2607 T2917 T2915 arg1Of sucrose,0.2
R2608 T2919 T2918 arg2Of added,was
R2609 T2919 T2920 arg1Of added,to
R2610 T2919 T2927 arg1Of added,(
R2611 T2923 T2920 arg2Of water,to
R2612 T2923 T2921 arg1Of water,the
R2613 T2923 T2922 arg1Of water,drinking
R2614 T2923 T2924 arg1Of water,of
R2615 T2926 T2924 arg2Of groups,of
R2616 T2926 T2925 arg1Of groups,all
R2617 T2930 T2927 arg2Of Waaji,(
R2618 T2930 T2928 arg1Of Waaji,Van
R2619 T2930 T2929 arg1Of Waaji,der
R2620 T2930 T2931 arg1Of Waaji,et
R2621 T2931 T2932 arg1Of et,al.
R2622 T2931 T2933 arg1Of et,","
R2623 T2931 T2934 arg1Of et,[
R2624 T2935 T2934 arg2Of 23,[
R2625 T2936 T2934 arg3Of ],[
R2626 T2937 T2927 arg3Of ),(
R2627 T2940 T2938 arg1Of solutions,Fresh
R2628 T2940 T2939 arg1Of solutions,carrageenan
R2629 T2940 T2941 arg1Of solutions,were
R2630 T2940 T2942 arg2Of solutions,prepared
R2631 T2942 T2941 arg2Of prepared,were
R2632 T2942 T2943 arg1Of prepared,daily
R2772 T3130 T3131 arg1Of Evaluation,of
R2773 T3132 T3131 arg2Of Colitis,of
R2774 T3134 T3133 arg1Of weight,Body
R2775 T3134 T3135 arg1Of weight,","
R2776 T3135 T3140 arg1Of ",",","
R2777 T3136 T3137 arg1Of liquid,and
R2778 T3138 T3137 arg2Of food,and
R2779 T3139 T3135 arg2Of consumption,","
R2780 T3139 T3136 arg1Of consumption,liquid
R2781 T3139 T3138 arg1Of consumption,food
R2782 T3140 T3142 arg1Of ",",and
R2783 T3141 T3140 arg2Of diarrhea,","
R2784 T3142 T3151 arg1Of and,were
R2785 T3142 T3152 arg2Of and,recorded
R2786 T3144 T3142 arg2Of bleeding,and
R2787 T3144 T3143 arg1Of bleeding,rectal
R2788 T3144 T3145 arg1Of bleeding,(
R2789 T3146 T3145 arg2Of detected,(
R2790 T3149 T3146 arg1Of inspection,detected
R2791 T3149 T3147 arg2Of inspection,by
R2792 T3149 T3148 arg1Of inspection,eye
R2793 T3150 T3145 arg3Of ),(
R2794 T3152 T3151 arg2Of recorded,were
R2795 T3152 T3153 arg1Of recorded,throughout
R2796 T3156 T3153 arg2Of period,throughout
R2797 T3156 T3154 arg1Of period,the
R2798 T3156 T3155 arg1Of period,feeding
R2799 T3159 T3157 arg2Of days,After
R2800 T3159 T3158 arg1Of days,55
R2801 T3161 T3162 arg1Of animals,were
R2802 T3161 T3163 arg2Of animals,sacrificed
R2803 T3163 T3157 arg1Of sacrificed,After
R2804 T3163 T3160 arg1Of sacrificed,","
R2805 T3163 T3162 arg2Of sacrificed,were
R2806 T3166 T3163 arg1Of dislocation,sacrificed
R2807 T3166 T3164 arg2Of dislocation,by
R2808 T3166 T3165 arg1Of dislocation,cervical
R2809 T3168 T3167 arg1Of length,The
R2810 T3168 T3169 arg1Of length,of
R2811 T3168 T3172 arg1Of length,was
R2812 T3168 T3173 arg2Of length,measured
R2813 T3171 T3169 arg2Of colon,of
R2814 T3171 T3170 arg1Of colon,the
R2815 T3173 T3172 arg2Of measured,was
R2816 T3173 T3174 arg1Of measured,as
R2817 T3173 T3180 arg1Of measured,[
R2818 T3175 T3174 arg2Of described,as
R2819 T3177 T3175 arg1Of Okayashu,described
R2820 T3177 T3176 arg2Of Okayashu,by
R2821 T3177 T3179 arg1Of Okayashu,al.
R2822 T3179 T3178 arg1Of al.,et
R2823 T3181 T3180 arg2Of 24,[
R2824 T3182 T3180 arg3Of ],[
R2825 T3186 T3185 arg1Of colon,each
R2826 T3186 T3187 arg1Of colon,was
R2827 T3186 T3188 arg2Of colon,ligated
R2828 T3188 T3187 arg2Of ligated,was
R2829 T3188 T3189 arg1Of ligated,in
R2830 T3188 T3194 arg1Of ligated,and
R2831 T3190 T3189 arg2Of sections,in
R2832 T3190 T3191 arg1Of sections,of
R2833 T3193 T3191 arg2Of cm,of
R2834 T3193 T3192 arg1Of cm,2
R2835 T3194 T3183 arg1Of and,Then
R2836 T3194 T3184 arg1Of and,","
R2837 T3195 T3196 arg1Of 1,to
R2838 T3198 T3196 arg2Of ml,to
R2839 T3198 T3197 arg1Of ml,2
R2840 T3200 T3201 arg1Of 10,%
R2841 T3202 T3195 arg1Of formalin,1
R2842 T3202 T3199 arg1Of formalin,of
R2843 T3202 T3200 arg1Of formalin,10
R2844 T3202 T3203 arg1Of formalin,was
R2845 T3202 T3204 arg2Of formalin,infused
R2846 T3204 T3194 arg2Of infused,and
R2847 T3204 T3203 arg2Of infused,was
R2848 T3204 T3205 arg1Of infused,into
R2849 T3208 T3205 arg2Of lumen,into
R2850 T3208 T3206 arg1Of lumen,the
R2851 T3208 T3207 arg1Of lumen,intestinal
R2852 T3211 T3210 arg1Of distended,moderately
R2853 T3212 T3209 arg1Of segment,The
R2854 T3212 T3211 arg1Of segment,distended
R2855 T3212 T3213 arg1Of segment,was
R2856 T3212 T3214 arg2Of segment,sectioned
R2857 T3212 T3216 arg2Of segment,fixed
R2858 T3214 T3215 arg1Of sectioned,and
R2859 T3215 T3213 arg2Of and,was
R2860 T3215 T3217 arg1Of and,in
R2861 T3216 T3215 arg2Of fixed,and
R2862 T3218 T3219 arg1Of 10,%
R2863 T3220 T3217 arg2Of formalin,in
R2864 T3220 T3218 arg1Of formalin,10
R2865 T3223 T3221 arg1Of day,The
R2866 T3223 T3222 arg1Of day,following
R2867 T3227 T3225 arg1Of content,the
R2868 T3227 T3226 arg1Of content,intestinal
R2869 T3227 T3228 arg1Of content,was
R2870 T3227 T3229 arg2Of content,removed
R2871 T3229 T3223 arg1Of removed,day
R2872 T3229 T3224 arg1Of removed,","
R2873 T3229 T3228 arg2Of removed,was
R2874 T3231 T3229 arg1Of vortexing,removed
R2875 T3231 T3230 arg2Of vortexing,by
R2876 T3234 T3232 arg1Of segment,The
R2877 T3234 T3233 arg2Of segment,fixed
R2878 T3234 T3235 arg1Of segment,was
R2879 T3234 T3236 arg2Of segment,kept
R2880 T3236 T3235 arg2Of kept,was
R2881 T3236 T3237 arg1Of kept,in
R2882 T3236 T3241 arg1Of kept,at
R2883 T3236 T3243 arg1Of kept,until
R2884 T3238 T3239 arg1Of 10,%
R2885 T3240 T3237 arg2Of formalin,in
R2886 T3240 T3238 arg1Of formalin,10
R2887 T3242 T3241 arg2Of 4°C,at
R2888 T3247 T3243 arg2Of procedure,until
R2889 T3247 T3244 arg1Of procedure,the
R2890 T3247 T3245 arg1Of procedure,paraffin
R2891 T3247 T3246 arg1Of procedure,embedding
R2892 T3249 T3248 arg1Of evaluate,To
R2893 T3251 T3249 arg2Of degree,evaluate
R2894 T3251 T3250 arg1Of degree,the
R2895 T3251 T3252 arg1Of degree,of
R2896 T3253 T3252 arg2Of inflammation,of
R2897 T3256 T3255 arg1Of segment,this
R2898 T3256 T3257 arg1Of segment,of
R2899 T3256 T3259 arg1Of segment,was
R2900 T3256 T3260 arg2Of segment,opened
R2901 T3258 T3257 arg2Of colon,of
R2902 T3260 T3259 arg2Of opened,was
R2903 T3260 T3261 arg1Of opened,longitudinally
R2904 T3263 T3264 arg1Of macroscopic,and
R2905 T3265 T3264 arg2Of histological,and
R2906 T3266 T3262 arg1Of scores,and
R2907 T3266 T3263 arg1Of scores,macroscopic
R2908 T3266 T3265 arg1Of scores,histological
R2909 T3266 T3267 arg1Of scores,of
R2910 T3266 T3269 arg1Of scores,were
R2911 T3266 T3270 arg2Of scores,recorded
R2912 T3268 T3267 arg2Of inflammation,of
R2913 T3270 T3248 modOf recorded,To
R2914 T3270 T3254 arg1Of recorded,","
R2915 T3270 T3259 modOf recorded,was
R2916 T3270 T3269 arg2Of recorded,were
R2917 T3270 T3271 arg1Of recorded,as
R2918 T3270 T3277 arg1Of recorded,","
R2919 T3270 T3278 arg1Of recorded,[
R2920 T3273 T3272 arg1Of described,previously
R2921 T3275 T3271 arg2Of 25,as
R2922 T3275 T3273 arg1Of 25,described
R2923 T3275 T3274 arg2Of 25,[
R2924 T3276 T3274 arg3Of ],[
R2925 T3279 T3278 arg2Of 26,[
R2926 T3280 T3278 arg3Of ],[
R2927 T3284 T3281 arg1Of staining,The
R2928 T3284 T3282 arg1Of staining,toluidine
R2929 T3284 T3283 arg1Of staining,blue
R2930 T3284 T3285 arg1Of staining,was
R2931 T3284 T3286 arg2Of staining,used
R2932 T3286 T3285 arg2Of used,was
R2933 T3286 T3287 arg1Of used,for
R2934 T3288 T3287 arg2Of identification,for
R2935 T3288 T3289 arg1Of identification,of
R2936 T3288 T3292 arg1Of identification,in
R2937 T3291 T3289 arg2Of polysaccharides,of
R2938 T3291 T3290 arg2Of polysaccharides,sulphated
R2939 T3295 T3292 arg2Of mucosa,in
R2940 T3295 T3293 arg1Of mucosa,the
R2941 T3295 T3294 arg1Of mucosa,intestinal
R2942 T3298 T3296 arg2Of day,On
R2943 T3298 T3297 arg1Of day,the
R2944 T3298 T3299 arg1Of day,of
R2945 T3300 T3299 arg2Of sacrifice,of
R2946 T3304 T3302 arg1Of sample,a
R2947 T3304 T3303 arg1Of sample,fresh
R2948 T3304 T3305 arg1Of sample,of
R2949 T3304 T3312 arg1Of sample,was
R2950 T3304 T3313 arg2Of sample,collected
R2951 T3307 T3305 arg2Of colon,of
R2952 T3307 T3306 arg1Of colon,each
R2953 T3307 T3308 arg1Of colon,(
R2954 T3310 T3308 arg2Of mg,(
R2955 T3310 T3309 arg1Of mg,50
R2956 T3311 T3308 arg3Of ),(
R2957 T3313 T3296 arg1Of collected,On
R2958 T3313 T3301 arg1Of collected,","
R2959 T3313 T3312 arg2Of collected,was
R2960 T3313 T3314 arg1Of collected,for
R2961 T3313 T3320 arg1Of collected,according
R2962 T3313 T3325 arg1Of collected,","
R2963 T3313 T3326 arg1Of collected,[
R2964 T3317 T3316 arg2Of MPO,(
R2965 T3318 T3316 arg3Of ),(
R2966 T3319 T3314 arg2Of assay,for
R2967 T3319 T3315 arg1Of assay,myeloperoxidase
R2968 T3319 T3316 arg1Of assay,(
R2969 T3321 T3320 arg2Of to,according
R2970 T3322 T3321 arg2Of Krawisz,to
R2971 T3322 T3323 arg1Of Krawisz,et
R2972 T3322 T3324 arg1Of Krawisz,al.
R2973 T3327 T3326 arg2Of 27,[
R2974 T3328 T3326 arg3Of ],[
R2975 T3330 T3329 arg1Of level,The
R2976 T3330 T3331 arg1Of level,of
R2977 T3330 T3333 arg1Of level,","
R2978 T3330 T3335 arg2Of level,expressed
R2979 T3330 T3339 arg1Of level,indicates
R2980 T3332 T3331 arg2Of MPO,of
R2981 T3335 T3334 arg1Of expressed,mainly
R2982 T3337 T3335 arg1Of neutrophils,expressed
R2983 T3337 T3336 arg2Of neutrophils,by
R2984 T3339 T3338 arg1Of indicates,","
R2985 T3341 T3339 arg2Of rate,indicates
R2986 T3341 T3340 arg1Of rate,the
R2987 T3341 T3342 arg1Of rate,of
R2988 T3343 T3342 arg2Of recruitment,of
R2989 T3343 T3344 arg1Of recruitment,of
R2990 T3343 T3346 arg1Of recruitment,to
R2991 T3345 T3344 arg2Of neutrophils,of
R2992 T3349 T3346 arg2Of mucosa,to
R2993 T3349 T3347 arg1Of mucosa,the
R2994 T3349 T3348 arg1Of mucosa,intestinal
R2995 T3351 T3350 arg1Of unit,One
R2996 T3351 T3352 arg1Of unit,of
R2997 T3351 T3355 arg1Of unit,corresponds
R2998 T3354 T3352 arg2Of activity,of
R2999 T3354 T3353 arg1Of activity,MPO
R3000 T3355 T3356 arg1Of corresponds,to
R3001 T3358 T3356 arg2Of degradation,to
R3002 T3358 T3357 arg1Of degradation,the
R3003 T3358 T3359 arg1Of degradation,of
R3004 T3361 T3359 arg2Of µmol,of
R3005 T3361 T3360 arg1Of µmol,1
R3006 T3361 T3362 arg1Of µmol,of
R3007 T3361 T3364 arg1Of µmol,per
R3008 T3361 T3366 arg1Of µmol,at
R3009 T3363 T3362 arg2Of peroxide,of
R3010 T3365 T3364 arg2Of minute,per
R3011 T3367 T3366 arg2Of 25°C,at
R3275 T3677 T3676 arg1Of Culture,Cell
R3276 T3681 T3678 arg1Of reagents,All
R3277 T3681 T3679 arg1Of reagents,tissue
R3278 T3681 T3680 arg1Of reagents,culture
R3279 T3681 T3682 arg1Of reagents,were
R3280 T3681 T3683 arg1Of reagents,from
R3281 T3683 T3682 arg2Of from,were
R3282 T3684 T3683 arg2Of Invitrogen,from
R3283 T3684 T3685 arg1Of Invitrogen,(
R3284 T3687 T3685 arg2Of Pontoise,(
R3285 T3687 T3686 arg1Of Pontoise,Cergy
R3286 T3687 T3688 arg1Of Pontoise,","
R3287 T3689 T3688 arg2Of France,","
R3288 T3690 T3685 arg3Of ),(
R3289 T3694 T3691 arg1Of cells,THP-1
R3290 T3694 T3692 arg1Of cells,human
R3291 T3694 T3693 arg1Of cells,monocytic
R3292 T3694 T3695 arg1Of cells,were
R3293 T3694 T3696 arg2Of cells,maintained
R3294 T3696 T3695 arg2Of maintained,were
R3295 T3696 T3697 arg1Of maintained,in
R3296 T3698 T3697 arg2Of RPMI-1640,in
R3297 T3698 T3699 arg2Of RPMI-1640,supplemented
R3298 T3699 T3700 arg1Of supplemented,with
R3299 T3701 T3702 arg1Of 10,%
R3300 T3703 T3701 arg1Of FCS,10
R3301 T3703 T3704 arg1Of FCS,","
R3302 T3704 T3709 arg1Of ",",","
R3303 T3705 T3706 arg1Of 2,mM
R3304 T3708 T3704 arg2Of -glutamine,","
R3305 T3708 T3705 arg1Of -glutamine,2
R3306 T3708 T3707 arg1Of -glutamine,L
R3307 T3709 T3713 arg1Of ",",and
R3308 T3710 T3711 arg1Of 50,U/ml
R3309 T3712 T3709 arg2Of penicillin,","
R3310 T3712 T3710 arg1Of penicillin,50
R3311 T3713 T3700 arg2Of and,with
R3312 T3714 T3715 arg1Of 50,mg/ml
R3313 T3716 T3713 arg2Of streptomycin,and
R3314 T3716 T3714 arg1Of streptomycin,50
R3315 T3716 T3717 arg1Of streptomycin,at
R3316 T3716 T3719 arg1Of streptomycin,in
R3317 T3718 T3717 arg2Of 37°C,at
R3318 T3721 T3722 arg1Of 5,%
R3319 T3724 T3719 arg2Of incubator,in
R3320 T3724 T3720 arg1Of incubator,a
R3321 T3724 T3721 arg1Of incubator,5
R3322 T3724 T3723 arg1Of incubator,CO2
R3323 T3729 T3725 arg1Of cells,Human
R3324 T3729 T3726 arg1Of cells,peripheral
R3325 T3729 T3727 arg1Of cells,blood
R3326 T3729 T3728 arg1Of cells,mononuclear
R3327 T3729 T3730 arg1Of cells,were
R3328 T3729 T3731 arg2Of cells,obtained
R3329 T3731 T3730 arg2Of obtained,were
R3330 T3731 T3732 arg1Of obtained,from
R3331 T3734 T3732 arg2Of blood,from
R3332 T3734 T3733 arg2Of blood,heparinized
R3333 T3738 T3731 arg1Of gradient,obtained
R3334 T3738 T3735 arg2Of gradient,by
R3335 T3738 T3736 arg1Of gradient,Ficoll-Hypaque
R3336 T3738 T3737 arg1Of gradient,density
R3337 T3739 T3740 arg1Of Monocytes,were
R3338 T3739 T3742 arg2Of Monocytes,isolated
R3339 T3742 T3740 arg2Of isolated,were
R3340 T3742 T3741 arg1Of isolated,then
R3341 T3742 T3743 arg1Of isolated,by
R3342 T3742 T3745 arg1Of isolated,to
R3343 T3742 T3748 arg1Of isolated,as
R3344 T3744 T3743 arg2Of adherence,by
R3345 T3747 T3745 arg2Of flasks,to
R3346 T3747 T3746 arg1Of flasks,culture
R3347 T3749 T3748 arg2Of described,as
R3348 T3749 T3750 arg1Of described,[
R3349 T3751 T3750 arg2Of 28,[
R3350 T3752 T3750 arg3Of ],[
R3351 T3755 T3753 arg2Of aggregation,For
R3352 T3755 T3754 arg1Of aggregation,cell
R3353 T3757 T3758 arg1Of monocytes,were
R3354 T3757 T3759 arg2Of monocytes,cultured
R3355 T3759 T3753 arg1Of cultured,For
R3356 T3759 T3756 arg1Of cultured,","
R3357 T3759 T3758 arg2Of cultured,were
R3358 T3759 T3760 arg1Of cultured,in
R3359 T3762 T3763 arg1Of presence,or
R3360 T3763 T3760 arg2Of or,in
R3361 T3763 T3761 arg1Of or,the
R3362 T3763 T3765 arg1Of or,of
R3363 T3764 T3763 arg2Of absence,or
R3364 T3766 T3767 arg1Of C10,or
R3365 T3767 T3765 arg2Of or,of
R3366 T3767 T3769 arg1Of or,for
R3367 T3768 T3767 arg2Of C40,or
R3368 T3771 T3769 arg2Of h,for
R3369 T3771 T3770 arg1Of h,72
R3370 T3773 T3772 arg1Of colonies,Cell
R3371 T3773 T3774 arg1Of colonies,were
R3372 T3773 T3775 arg2Of colonies,monitored
R3373 T3775 T3774 arg2Of monitored,were
R3374 T3775 T3776 arg1Of monitored,under
R3375 T3781 T3776 arg2Of microscope,under
R3376 T3781 T3777 arg1Of microscope,an
R3377 T3781 T3778 arg2Of microscope,inverted
R3378 T3781 T3779 arg1Of microscope,phase
R3379 T3781 T3780 arg1Of microscope,contrast
R3380 T3781 T3782 arg2Of microscope,coupled
R3381 T3782 T3783 arg1Of coupled,through
R3382 T3786 T3783 arg2Of camera,through
R3383 T3786 T3784 arg1Of camera,a
R3384 T3786 T3785 arg1Of camera,video
R3385 T3786 T3787 arg1Of camera,to
R3386 T3789 T3787 arg2Of computer,to
R3387 T3789 T3788 arg1Of computer,a
R3388 T3792 T3790 arg2Of wells,In
R3389 T3792 T3791 arg1Of wells,some
R3390 T3794 T3813 arg1Of neutralizing,was
R3391 T3794 T3814 arg2Of neutralizing,added
R3392 T3796 T3794 arg2Of antibody,neutralizing
R3393 T3796 T3795 arg1Of antibody,monoclonal
R3394 T3796 T3797 arg1Of antibody,to
R3395 T3798 T3797 arg2Of ICAM-1,to
R3396 T3798 T3799 arg1Of ICAM-1,(
R3397 T3798 T3803 arg1Of ICAM-1,(
R3398 T3801 T3799 arg2Of µg/ml,(
R3399 T3801 T3800 arg1Of µg/ml,2.5
R3400 T3802 T3799 arg3Of ),(
R3401 T3804 T3803 arg2Of Tebu,(
R3402 T3804 T3805 arg1Of Tebu,","
R3403 T3804 T3810 arg1Of Tebu,","
R3404 T3809 T3805 arg2Of Yvelines,","
R3405 T3809 T3806 arg1Of Yvelines,Le
R3406 T3809 T3807 arg1Of Yvelines,Perray
R3407 T3809 T3808 arg1Of Yvelines,en
R3408 T3811 T3810 arg2Of France,","
R3409 T3812 T3803 arg3Of ),(
R3410 T3814 T3790 arg1Of added,In
R3411 T3814 T3793 arg1Of added,","
R3412 T3814 T3813 arg2Of added,was
R3563 T3988 T3986 arg1Of Analysis,Cell
R3564 T3988 T3987 arg1Of Analysis,Cycle
R3565 T3990 T3989 arg1Of cells,THP-1
R3566 T3990 T3991 arg1Of cells,in
R3567 T3990 T3995 arg1Of cells,were
R3568 T3990 T3996 arg2Of cells,exposed
R3569 T3990 T4011 arg1Of cells,being
R3570 T3990 T4012 arg2Of cells,stained
R3571 T3990 T4016 arg1Of cells,using
R3572 T3994 T3991 arg2Of phase,in
R3573 T3994 T3992 arg1Of phase,exponential
R3574 T3994 T3993 arg1Of phase,growth
R3575 T3996 T3995 arg2Of exposed,were
R3576 T3996 T3997 arg1Of exposed,to
R3577 T3996 T4000 arg1Of exposed,in
R3578 T3996 T4010 arg1Of exposed,before
R3579 T3999 T3997 arg2Of medium,to
R3580 T3999 T3998 arg1Of medium,complete
R3581 T4002 T4003 arg1Of presence,or
R3582 T4003 T4000 arg2Of or,in
R3583 T4003 T4001 arg1Of or,the
R3584 T4003 T4005 arg1Of or,of
R3585 T4003 T4007 arg1Of or,for
R3586 T4004 T4003 arg2Of absence,or
R3587 T4006 T4005 arg2Of carrageenans,of
R3588 T4009 T4007 arg2Of h,for
R3589 T4009 T4008 arg1Of h,24
R3590 T4012 T4010 arg2Of stained,before
R3591 T4012 T4011 arg2Of stained,being
R3592 T4012 T4013 arg1Of stained,with
R3593 T4012 T4016 modOf stained,using
R3594 T4015 T4013 arg2Of iodide,with
R3595 T4015 T4014 arg1Of iodide,propidium
R3596 T4016 T4021 arg1Of using,according
R3597 T4016 T4027 arg1Of using,(
R3598 T4020 T4016 arg2Of kit,using
R3599 T4020 T4017 arg1Of kit,the
R3600 T4020 T4018 arg1Of kit,DNA-Prep
R3601 T4020 T4019 arg1Of kit,Coulter
R3602 T4022 T4021 arg2Of to,according
R3603 T4024 T4023 arg1Of manufacturer,the
R3604 T4024 T4025 arg2Of manufacturer,'s
R3605 T4026 T4022 arg2Of instruction,to
R3606 T4026 T4025 arg1Of instruction,'s
R3607 T4028 T4029 arg1Of Beckman-Coulter,","
R3608 T4029 T4031 arg1Of ",",","
R3609 T4030 T4029 arg2Of Villepinte,","
R3610 T4031 T4027 arg2Of ",",(
R3611 T4032 T4031 arg2Of France,","
R3612 T4033 T4027 arg3Of ),(
R3613 T4036 T4034 arg1Of content,Cell
R3614 T4036 T4035 arg1Of content,DNA
R3615 T4036 T4037 arg1Of content,was
R3616 T4036 T4039 arg2Of content,analyzed
R3617 T4039 T4037 arg2Of analyzed,was
R3618 T4039 T4038 arg1Of analyzed,then
R3619 T4042 T4039 arg1Of cytometry,analyzed
R3620 T4042 T4040 arg2Of cytometry,by
R3621 T4042 T4041 arg1Of cytometry,flow
R3622 T4042 T4043 arg1Of cytometry,using
R3623 T4046 T4043 arg2Of XL2,using
R3624 T4046 T4044 arg1Of XL2,an
R3625 T4046 T4045 arg1Of XL2,EPICS
R3626 T4046 T4047 arg1Of XL2,(
R3627 T4048 T4047 arg2Of Beckman-Coulter,(
R3628 T4049 T4047 arg3Of ),(
R3629 T4051 T4050 arg1Of data,Raw
R3630 T4051 T4052 arg1Of data,for
R3631 T4051 T4065 arg1Of data,were
R3632 T4051 T4066 arg2Of data,expressed
R3633 T4054 T4052 arg2Of distribution,for
R3634 T4054 T4053 arg1Of distribution,the
R3635 T4054 T4055 arg1Of distribution,of
R3636 T4057 T4055 arg2Of content,of
R3637 T4057 T4056 arg1Of content,DNA
R3638 T4057 T4058 arg1Of content,of
R3639 T4060 T4058 arg2Of cells,of
R3640 T4060 T4059 arg1Of cells,"30,000"
R3641 T4060 T4061 arg2Of cells,retrieved
R3642 T4061 T4062 arg1Of retrieved,from
R3643 T4064 T4062 arg2Of cytometer,from
R3644 T4064 T4063 arg1Of cytometer,the
R3645 T4066 T4065 arg2Of expressed,were
R3646 T4066 T4067 arg1Of expressed,as
R3647 T4069 T4067 arg2Of percentage,as
R3648 T4069 T4068 arg1Of percentage,the
R3649 T4069 T4070 arg1Of percentage,of
R3650 T4069 T4072 arg1Of percentage,through
R3651 T4071 T4070 arg2Of G0/G1,of
R3652 T4074 T4072 arg2Of populations,through
R3653 T4074 T4073 arg1Of populations,G2/M
R3654 T4077 T4075 arg1Of software,Multicycle
R3655 T4077 T4076 arg1Of software,AV
R3656 T4077 T4078 arg1Of software,(
R3657 T4077 T4088 arg1Of software,was
R3658 T4077 T4089 arg2Of software,used
R3659 T4077 T4097 arg1Of software,facilitate
R3660 T4081 T4078 arg2Of Systems,(
R3661 T4081 T4079 arg1Of Systems,Phoenix
R3662 T4081 T4080 arg1Of Systems,Flow
R3663 T4081 T4082 arg1Of Systems,","
R3664 T4084 T4082 arg2Of Diego,","
R3665 T4084 T4083 arg1Of Diego,San
R3666 T4084 T4085 arg1Of Diego,","
R3667 T4086 T4085 arg2Of CA,","
R3668 T4087 T4078 arg3Of ),(
R3669 T4089 T4088 arg2Of used,was
R3670 T4089 T4090 modOf used,to
R3671 T4089 T4096 arg1Of used,and
R3672 T4091 T4090 arg1Of generate,to
R3673 T4095 T4091 arg2Of histograms,generate
R3674 T4095 T4092 arg1Of histograms,DNA
R3675 T4095 T4093 arg1Of histograms,content
R3676 T4095 T4094 arg1Of histograms,frequency
R3677 T4097 T4096 arg2Of facilitate,and
R3678 T4099 T4097 arg2Of analysis,facilitate
R3679 T4099 T4098 arg1Of analysis,data
R3798 T4244 T4242 arg1Of Antigen,Cell
R3799 T4244 T4243 arg1Of Antigen,Surface
R3800 T4246 T4244 arg1Of Analysis,Antigen
R3801 T4246 T4245 arg1Of Analysis,Expression
R3802 T4249 T4247 arg1Of Monocytes,Peripheral
R3803 T4249 T4248 arg1Of Monocytes,Blood
R3804 T4249 T4250 arg1Of Monocytes,or
R3805 T4250 T4253 arg1Of or,were
R3806 T4250 T4254 arg2Of or,exposed
R3807 T4252 T4250 arg2Of cells,or
R3808 T4252 T4251 arg1Of cells,THP-1
R3809 T4254 T4253 arg2Of exposed,were
R3810 T4254 T4255 arg1Of exposed,to
R3811 T4254 T4258 arg1Of exposed,in
R3812 T4257 T4255 arg2Of medium,to
R3813 T4257 T4256 arg1Of medium,complete
R3814 T4260 T4261 arg1Of presence,or
R3815 T4261 T4258 arg2Of or,in
R3816 T4261 T4259 arg1Of or,the
R3817 T4261 T4263 arg1Of or,of
R3818 T4261 T4265 arg1Of or,for
R3819 T4262 T4261 arg2Of absence,or
R3820 T4264 T4263 arg2Of carrageenan,of
R3821 T4267 T4265 arg2Of h,for
R3822 T4267 T4266 arg1Of h,36
R3823 T4270 T4268 arg2Of washes,After
R3824 T4270 T4269 arg1Of washes,two
R3825 T4270 T4271 arg1Of washes,in
R3826 T4270 T4273 arg1Of washes,without
R3827 T4272 T4271 arg2Of PBS,in
R3828 T4274 T4275 arg1Of Ca2+,and
R3829 T4275 T4273 arg2Of and,without
R3830 T4276 T4275 arg2Of Mg2+,and
R3831 T4278 T4279 arg1Of cells,were
R3832 T4278 T4280 arg2Of cells,incubated
R3833 T4280 T4268 arg1Of incubated,After
R3834 T4280 T4277 arg1Of incubated,","
R3835 T4280 T4279 arg2Of incubated,were
R3836 T4280 T4281 arg1Of incubated,in
R3837 T4280 T4293 modOf incubated,to
R3838 T4282 T4281 arg2Of PBS,in
R3839 T4282 T4283 arg1Of PBS,containing
R3840 T4284 T4285 arg1Of 0.1,%
R3841 T4286 T4284 arg1Of gelatin,0.1
R3842 T4286 T4287 arg1Of gelatin,and
R3843 T4287 T4283 arg2Of and,containing
R3844 T4288 T4289 arg1Of 8,%
R3845 T4292 T4287 arg2Of serum,and
R3846 T4292 T4288 arg1Of serum,8
R3847 T4292 T4290 arg1Of serum,AB
R3848 T4292 T4291 arg1Of serum,human
R3849 T4294 T4293 arg1Of prevent,to
R3850 T4295 T4294 arg2Of binding,prevent
R3851 T4295 T4296 arg1Of binding,to
R3852 T4298 T4296 arg2Of receptors,to
R3853 T4298 T4297 arg1Of receptors,Fc
R3854 T4302 T4301 arg1Of cells,5×105
R3855 T4302 T4303 arg1Of cells,were
R3856 T4302 T4304 arg2Of cells,incubated
R3857 T4304 T4299 arg1Of incubated,Then
R3858 T4304 T4300 arg1Of incubated,","
R3859 T4304 T4303 arg2Of incubated,were
R3860 T4304 T4305 arg1Of incubated,with
R3861 T4304 T4308 arg1Of incubated,at
R3862 T4304 T4310 arg1Of incubated,for
R3863 T4307 T4305 arg2Of antibodies,with
R3864 T4307 T4306 arg1Of antibodies,primary
R3865 T4309 T4308 arg2Of 4°C,at
R3866 T4312 T4310 arg2Of min,for
R3867 T4312 T4311 arg1Of min,30
R3868 T4315 T4313 arg1Of washes,Two
R3869 T4315 T4314 arg1Of washes,other
R3870 T4315 T4316 arg1Of washes,in
R3871 T4315 T4318 arg1Of washes,preceded
R3872 T4317 T4316 arg2Of PBS,in
R3873 T4319 T4318 arg2Of incubation,preceded
R3874 T4319 T4320 arg1Of incubation,with
R3875 T4325 T4320 arg2Of IgG,with
R3876 T4325 T4321 arg1Of IgG,FITC-conjugated
R3877 T4325 T4322 arg1Of IgG,goat
R3878 T4325 T4323 arg1Of IgG,antibody
R3879 T4325 T4324 arg1Of IgG,anti-mouse
R3880 T4325 T4326 arg2Of IgG,diluted
R3881 T4326 T4328 arg1Of diluted,at
R3882 T4326 T4330 arg1Of diluted,for
R3883 T4327 T4326 arg3Of 1/1000,diluted
R3884 T4329 T4328 arg2Of 4°C,at
R3885 T4332 T4330 arg2Of min,for
R3886 T4332 T4331 arg1Of min,30
R3887 T4332 T4333 arg1Of min,(
R3888 T4334 T4333 arg2Of Tebu,(
R3889 T4335 T4333 arg3Of ),(
R3890 T4339 T4336 arg2Of washes,After
R3891 T4339 T4337 arg1Of washes,two
R3892 T4339 T4338 arg1Of washes,additional
R3893 T4341 T4342 arg1Of analysis,of
R3894 T4341 T4345 arg1Of analysis,was
R3895 T4341 T4346 arg2Of analysis,performed
R3896 T4344 T4342 arg2Of cells,of
R3897 T4344 T4343 arg2Of cells,stained
R3898 T4346 T4336 arg1Of performed,After
R3899 T4346 T4340 arg1Of performed,","
R3900 T4346 T4345 arg2Of performed,was
R3901 T4346 T4347 arg1Of performed,on
R3902 T4350 T4347 arg2Of XL2,on
R3903 T4350 T4348 arg1Of XL2,an
R3904 T4350 T4349 arg1Of XL2,EPICS
R3905 T4350 T4351 arg1Of XL2,(
R3906 T4352 T4351 arg2Of Beckman-Coulter,(
R3907 T4353 T4351 arg3Of ),(
R3908 T4356 T4354 arg1Of population,The
R3909 T4356 T4355 arg1Of population,cell
R3910 T4356 T4357 arg1Of population,was
R3911 T4356 T4358 arg2Of population,gated
R3912 T4358 T4357 arg2Of gated,was
R3913 T4358 T4359 arg1Of gated,according
R3914 T4360 T4359 arg2Of to,according
R3915 T4362 T4363 arg1Of forward,and
R3916 T4364 T4363 arg2Of wide-angle,and
R3917 T4366 T4360 arg2Of scattering,to
R3918 T4366 T4361 arg1Of scattering,its
R3919 T4366 T4362 arg1Of scattering,forward
R3920 T4366 T4364 arg1Of scattering,wide-angle
R3921 T4366 T4365 arg1Of scattering,light
R3922 T4367 T4368 arg1Of Data,were
R3923 T4367 T4369 arg2Of Data,expressed
R3924 T4369 T4368 arg2Of expressed,were
R3925 T4369 T4370 arg1Of expressed,as
R3926 T4374 T4370 arg2Of intensity,as
R3927 T4374 T4371 arg1Of intensity,mean
R3928 T4374 T4372 arg1Of intensity,relative
R3929 T4374 T4373 arg1Of intensity,fluorescence
R3930 T4374 T4375 arg1Of intensity,(
R3931 T4374 T4378 arg1Of intensity,of
R3932 T4376 T4375 arg2Of MFI,(
R3933 T4377 T4375 arg3Of ),(
R3934 T4380 T4378 arg2Of cells,of
R3935 T4380 T4379 arg1Of cells,3000
R4092 T4589 T4587 arg1Of Bioassay,TNF
R4093 T4589 T4588 arg1Of Bioassay,Activity
R4094 T4590 T4591 arg1Of Monocytes,or
R4095 T4591 T4594 arg1Of or,were
R4096 T4591 T4595 arg2Of or,cultured
R4097 T4593 T4591 arg2Of cells,or
R4098 T4593 T4592 arg1Of cells,THP-1
R4099 T4595 T4594 arg2Of cultured,were
R4100 T4595 T4596 arg1Of cultured,with
R4101 T4595 T4598 arg1Of cultured,without
R4102 T4596 T4597 arg1Of with,or
R4103 T4598 T4597 arg2Of without,or
R4104 T4600 T4596 arg2Of concentrations,with
R4105 T4600 T4598 arg2Of concentrations,without
R4106 T4600 T4599 arg1Of concentrations,different
R4107 T4600 T4601 arg1Of concentrations,of
R4108 T4600 T4611 arg1Of concentrations,for
R4109 T4602 T4603 arg1Of CGNs,or
R4110 T4603 T4601 arg2Of or,of
R4111 T4603 T4605 arg1Of or,(
R4112 T4604 T4603 arg2Of LPS,or
R4113 T4607 T4605 arg2Of typhosa,(
R4114 T4607 T4606 arg1Of typhosa,Salmonella
R4115 T4607 T4608 arg1Of typhosa,","
R4116 T4609 T4608 arg2Of Sigma,","
R4117 T4610 T4605 arg3Of ),(
R4118 T4613 T4612 arg1Of h,24
R4119 T4613 T4614 arg1Of h,or
R4120 T4614 T4611 arg2Of or,for
R4121 T4617 T4614 arg2Of time,or
R4122 T4617 T4615 arg1Of time,the
R4123 T4617 T4616 arg2Of time,indicated
R4124 T4619 T4618 arg1Of active,Biologically
R4125 T4620 T4619 arg1Of TNF-α/β,active
R4126 T4620 T4621 arg1Of TNF-α/β,in
R4127 T4620 T4625 arg1Of TNF-α/β,was
R4128 T4620 T4626 arg2Of TNF-α/β,measured
R4129 T4624 T4621 arg2Of supernatant,in
R4130 T4624 T4622 arg1Of supernatant,tissue
R4131 T4624 T4623 arg1Of supernatant,culture
R4132 T4626 T4625 arg2Of measured,was
R4133 T4627 T4626 arg3Of using,measured
R4134 T4627 T4635 arg1Of using,[
R4135 T4634 T4627 arg2Of assay,using
R4136 T4634 T4628 arg1Of assay,the
R4137 T4634 T4629 arg1Of assay,WEHI
R4138 T4634 T4630 arg1Of assay,164
R4139 T4634 T4631 arg1Of assay,clone
R4140 T4634 T4632 arg1Of assay,13-cell
R4141 T4634 T4633 arg1Of assay,killing
R4142 T4636 T4635 arg2Of 29,[
R4143 T4637 T4635 arg3Of ],[
R4144 T4639 T4638 arg1Of concentrations,TNF
R4145 T4639 T4640 arg1Of concentrations,are
R4146 T4639 T4641 arg2Of concentrations,expressed
R4147 T4641 T4640 arg2Of expressed,are
R4148 T4641 T4642 arg1Of expressed,as
R4149 T4643 T4642 arg2Of pg/ml,as
R4213 T4751 T4750 arg1Of Analysis,RT-PCR
R4214 T4753 T4752 arg1Of RNA,Total
R4215 T4753 T4754 arg1Of RNA,from
R4216 T4753 T4756 arg1Of RNA,was
R4217 T4753 T4757 arg2Of RNA,isolated
R4218 T4755 T4754 arg2Of monocytes,from
R4219 T4757 T4756 arg2Of isolated,was
R4220 T4758 T4757 arg3Of using,isolated
R4221 T4760 T4758 arg2Of Reagent™,using
R4222 T4760 T4759 arg1Of Reagent™,TRIzol
R4223 T4760 T4761 arg1Of Reagent™,(
R4224 T4762 T4761 arg2Of Invitrogen,(
R4225 T4763 T4761 arg3Of ),(
R4226 T4764 T4765 arg1Of . cD,NA
R4227 T4764 T4766 arg2Of . cD,was gener
R4228 T4766 T4756 modOf was gener,was
R4229 T4766 T4765 arg2Of was gener,NA
R4230 T4766 T4767 arg1Of was gener,at
R4231 T4766 T4780 arg1Of was gener,v
R4232 T4769 T4767 arg2Of d ,at
R4233 T4769 T4768 arg1Of d ,e
R4234 T4769 T4770 arg1Of d ,on
R4235 T4769 T4773 arg1Of d ,to
R4236 T4772 T4770 arg2Of of ,on
R4237 T4772 T4771 arg1Of of ,1 µg
R4238 T4776 T4773 arg2Of n a re,to
R4239 T4776 T4774 arg1Of n a re,t
R4240 T4776 T4775 arg1Of n a re,al RNA i
R4241 T4776 T4777 arg1Of n a re,ac
R4242 T4779 T4777 arg2Of on,ac
R4243 T4779 T4778 arg1Of on,ti
R4244 T4781 T4766 arg3Of olume,was gener
R4245 T4784 T4781 arg2Of ng M-MLV reve,olume
R4246 T4784 T4782 arg1Of ng M-MLV reve,of 20
R4247 T4784 T4783 arg1Of ng M-MLV reve,"µl, usi"
R4248 T4784 T4785 arg1Of ng M-MLV reve,r
R4249 T4786 T4785 arg2Of se transcr,r
R4250 T4787 T4785 arg3Of i,r
R4251 T4788 T4789 arg1Of PCR,was
R4252 T4788 T4790 arg2Of PCR,done
R4253 T4790 T4789 arg2Of done,was
R4254 T4790 T4791 arg1Of done,in
R4255 T4794 T4791 arg2Of range,in
R4256 T4794 T4792 arg1Of range,the
R4257 T4794 T4793 arg1Of range,linear
R4258 T4794 T4795 arg1Of range,of
R4259 T4796 T4795 arg2Of amplification,of
R4260 T4796 T4797 arg1Of amplification,(
R4261 T4798 T4797 arg2Of determined,(
R4262 T4798 T4799 arg1Of determined,for
R4263 T4803 T4799 arg2Of combination,for
R4264 T4803 T4800 arg1Of combination,each
R4265 T4803 T4801 arg1Of combination,primer
R4266 T4803 T4802 arg1Of combination,pair-cDNA
R4267 T4804 T4797 arg3Of ),(
R4268 T4807 T4805 arg1Of reactions,Standard
R4269 T4807 T4806 arg1Of reactions,PCR
R4270 T4807 T4808 arg1Of reactions,were
R4271 T4807 T4809 arg2Of reactions,performed
R4272 T4809 T4808 arg2Of performed,were
R4273 T4809 T4810 arg1Of performed,with
R4274 T4809 T4849 arg1Of performed,(
R4275 T4812 T4811 arg1Of µl,1
R4276 T4812 T4813 arg1Of µl,of
R4277 T4812 T4817 arg1Of µl,","
R4278 T4816 T4813 arg2Of solution,of
R4279 T4816 T4814 arg1Of solution,the
R4280 T4816 T4815 arg1Of solution,cDNA
R4281 T4817 T4824 arg1Of ",",","
R4282 T4819 T4817 arg2Of µM,","
R4283 T4819 T4818 arg1Of µM,50
R4284 T4819 T4820 arg1Of µM,of
R4285 T4823 T4820 arg2Of solution,of
R4286 T4823 T4821 arg1Of solution,each
R4287 T4823 T4822 arg1Of solution,primer
R4288 T4824 T4830 arg1Of ",",","
R4289 T4826 T4824 arg2Of mM,","
R4290 T4826 T4825 arg1Of mM,10
R4291 T4826 T4827 arg1Of mM,of
R4292 T4829 T4827 arg2Of dNTP,of
R4293 T4829 T4828 arg1Of dNTP,each
R4294 T4830 T4834 arg1Of ",",","
R4295 T4830 T4842 arg1Of ",",and
R4296 T4831 T4832 arg1Of 25,mM
R4297 T4833 T4830 arg2Of MgCl2,","
R4298 T4833 T4831 arg1Of MgCl2,25
R4299 T4836 T4835 arg1Of Goldstar,10X
R4300 T4840 T4834 arg2Of buffer,","
R4301 T4840 T4836 arg1Of buffer,Goldstar
R4302 T4840 T4837 arg1Of buffer,DNA
R4303 T4840 T4838 arg1Of buffer,polymerase
R4304 T4840 T4839 arg1Of buffer,reaction
R4305 T4842 T4810 arg2Of and,with
R4306 T4842 T4841 arg1Of and,","
R4307 T4844 T4842 arg2Of units,and
R4308 T4844 T4843 arg1Of units,0.5
R4309 T4844 T4845 arg1Of units,of
R4310 T4848 T4845 arg2Of polymerase,of
R4311 T4848 T4846 arg1Of polymerase,Goldstar
R4312 T4848 T4847 arg1Of polymerase,DNA
R4313 T4850 T4851 arg1Of Eurogentec,","
R4314 T4851 T4849 arg2Of ",",(
R4315 T4851 T4853 arg1Of ",",","
R4316 T4852 T4851 arg2Of Seraing,","
R4317 T4854 T4853 arg2Of Belgium,","
R4318 T4855 T4849 arg3Of ),(
R4319 T4858 T4856 arg1Of cycle,First
R4320 T4858 T4857 arg1Of cycle,PCR
R4321 T4858 T4859 arg1Of cycle,consisted
R4322 T4859 T4860 arg1Of consisted,of
R4323 T4859 T4863 arg1Of consisted,at
R4324 T4859 T4875 arg1Of consisted,;
R4325 T4862 T4860 arg2Of min,of
R4326 T4862 T4861 arg1Of min,1
R4327 T4864 T4865 arg1Of 92°C,","
R4328 T4865 T4870 arg1Of ",",and
R4329 T4867 T4865 arg2Of min,","
R4330 T4867 T4866 arg1Of min,1
R4331 T4867 T4868 arg1Of min,at
R4332 T4869 T4868 arg2Of 58°C,at
R4333 T4870 T4863 arg2Of and,at
R4334 T4870 T4873 arg1Of and,at
R4335 T4872 T4870 arg2Of min,and
R4336 T4872 T4871 arg1Of min,1
R4337 T4874 T4873 arg2Of 72°C,at
R4338 T4879 T4877 arg1Of cycle,each
R4339 T4879 T4878 arg1Of cycle,PCR
R4340 T4879 T4880 arg1Of cycle,consisted
R4341 T4880 T4875 arg2Of consisted,;
R4342 T4880 T4876 arg1Of consisted,then
R4343 T4880 T4881 arg1Of consisted,of
R4344 T4880 T4884 arg1Of consisted,at
R4345 T4883 T4881 arg2Of sec,of
R4346 T4883 T4882 arg1Of sec,40
R4347 T4885 T4884 arg2Of 92°C,at
R4348 T4888 T4887 arg1Of sec,40
R4349 T4888 T4889 arg1Of sec,at
R4350 T4888 T4894 arg1Of sec,at
R4351 T4888 T4899 arg1Of sec,was
R4352 T4888 T4900 arg2Of sec,amplified
R4353 T4888 T4941 arg1Of sec,sense
R4354 T4890 T4891 arg1Of 58°C,and
R4355 T4891 T4889 arg2Of and,at
R4356 T4893 T4891 arg2Of sec,and
R4357 T4893 T4892 arg1Of sec,50
R4358 T4896 T4894 arg2Of cDNA,at
R4359 T4896 T4895 arg1Of cDNA,72°C.
R4360 T4896 T4897 arg1Of cDNA,for
R4361 T4898 T4897 arg2Of β-actin,for
R4362 T4900 T4899 arg2Of amplified,was
R4363 T4900 T4901 arg1Of amplified,for
R4364 T4900 T4907 arg1Of amplified,:
R4365 T4903 T4901 arg2Of cycles,for
R4366 T4903 T4902 arg1Of cycles,28
R4367 T4903 T4904 arg1Of cycles,using
R4368 T4906 T4904 arg2Of oligos,using
R4369 T4906 T4905 arg1Of oligos,the
R4370 T4907 T4875 arg3Of :,;
R4371 T4907 T4886 arg1Of :,","
R4372 T4909 T4908 arg1Of 5′-GGCATCGTGATGGACTCCG-3′,sense
R4373 T4909 T4910 arg1Of 5′-GGCATCGTGATGGACTCCG-3′,and
R4374 T4910 T4914 arg1Of and,for
R4375 T4910 T4916 arg1Of and,was
R4376 T4910 T4917 arg2Of and,amplified
R4377 T4913 T4910 arg2Of cDNA,and
R4378 T4913 T4911 arg1Of cDNA,antisense
R4379 T4913 T4912 arg1Of cDNA,5′GCTGGAAGGTGGACAGCGA-3′.
R4380 T4915 T4914 arg2Of TNF-α,for
R4381 T4917 T4916 arg2Of amplified,was
R4382 T4917 T4918 arg1Of amplified,for
R4383 T4917 T4924 arg1Of amplified,:
R4384 T4920 T4918 arg2Of cycles,for
R4385 T4920 T4919 arg1Of cycles,35
R4386 T4920 T4921 arg1Of cycles,using
R4387 T4923 T4921 arg2Of oligos,using
R4388 T4923 T4922 arg1Of oligos,the
R4389 T4926 T4925 arg1Of 5′-AAGCCTGTAGCCCATGTTGT-3′,sense
R4390 T4926 T4927 arg1Of 5′-AAGCCTGTAGCCCATGTTGT-3′,and
R4391 T4927 T4931 arg1Of and,for
R4392 T4927 T4933 arg1Of and,was
R4393 T4927 T4934 arg2Of and,amplified
R4394 T4930 T4927 arg2Of cDNA,and
R4395 T4930 T4928 arg1Of cDNA,antisense
R4396 T4930 T4929 arg1Of cDNA,5′-CAGATAGATGGGCTCATACC-3′.
R4397 T4932 T4931 arg2Of ICAM-1,for
R4398 T4934 T4924 arg2Of amplified,:
R4399 T4934 T4933 arg2Of amplified,was
R4400 T4934 T4935 arg1Of amplified,for
R4401 T4937 T4935 arg2Of cycles,for
R4402 T4937 T4936 arg1Of cycles,35
R4403 T4937 T4938 arg1Of cycles,using
R4404 T4940 T4938 arg2Of oligos,using
R4405 T4940 T4939 arg1Of oligos,the
R4406 T4941 T4907 arg2Of sense,:
R4407 T4941 T4916 modOf sense,was
R4408 T4942 T4943 arg1Of 5′-GTAGCAGCCGCAGTCATAATGG-3′,and
R4409 T4944 T4943 arg2Of antisense,and
R4410 T4946 T4941 arg2Of TGCTGTTGTATCTGACTGAGG-3′,sense
R4411 T4946 T4942 arg1Of TGCTGTTGTATCTGACTGAGG-3′,5′-GTAGCAGCCGCAGTCATAATGG-3′
R4412 T4946 T4944 arg1Of TGCTGTTGTATCTGACTGAGG-3′,antisense
R4413 T4946 T4945 arg1Of TGCTGTTGTATCTGACTGAGG-3′,5′-A
R4654 T5243 T5239 arg1Of Assay,NF-kB
R4655 T5243 T5240 arg1Of Assay,Transcription
R4656 T5243 T5241 arg1Of Assay,Reporter
R4657 T5243 T5242 arg1Of Assay,Gene
R4658 T5246 T5244 arg1Of 3XMHC-luc,The
R4659 T5246 T5245 arg1Of 3XMHC-luc,plasmid
R4660 T5246 T5247 arg1Of 3XMHC-luc,(
R4661 T5246 T5267 arg1Of 3XMHC-luc,contains
R4662 T5250 T5248 arg1Of gift,a
R4663 T5250 T5249 arg1Of gift,generous
R4664 T5250 T5251 arg1Of gift,from
R4665 T5250 T5255 arg1Of gift,and
R4666 T5254 T5251 arg2Of Westwick,from
R4667 T5254 T5252 arg1Of Westwick,Drs.
R4668 T5254 T5253 arg1Of Westwick,J.
R4669 T5255 T5247 arg2Of and,(
R4670 T5257 T5255 arg2Of Brenner,and
R4671 T5257 T5256 arg1Of Brenner,D.A.
R4672 T5257 T5258 arg1Of Brenner,","
R4673 T5257 T5263 arg1Of Brenner,","
R4674 T5259 T5258 arg2Of University,","
R4675 T5259 T5260 arg1Of University,of
R4676 T5262 T5260 arg2Of Carolina,of
R4677 T5262 T5261 arg1Of Carolina,North
R4678 T5265 T5263 arg2Of Hill,","
R4679 T5265 T5264 arg1Of Hill,Chapel
R4680 T5266 T5247 arg3Of ),(
R4681 T5269 T5267 arg2Of copies,contains
R4682 T5269 T5268 arg1Of copies,three
R4683 T5269 T5270 arg1Of copies,of
R4684 T5269 T5273 arg1Of copies,from
R4685 T5269 T5279 arg1Of copies,","
R4686 T5269 T5280 arg2Of copies,placed
R4687 T5272 T5270 arg2Of element,of
R4688 T5272 T5271 arg1Of element,NF-κB-responsive
R4689 T5278 T5273 arg2Of locus,from
R4690 T5278 T5274 arg1Of locus,the
R4691 T5278 T5275 arg1Of locus,MHC
R4692 T5278 T5276 arg1Of locus,class
R4693 T5278 T5277 arg1Of locus,I
R4694 T5280 T5281 arg1Of placed,upstream
R4695 T5281 T5282 arg1Of upstream,of
R4696 T5285 T5282 arg2Of gene,of
R4697 T5285 T5283 arg1Of gene,the
R4698 T5285 T5284 arg1Of gene,luciferase
R4699 T5289 T5286 arg1Of cells,Human
R4700 T5289 T5287 arg1Of cells,monocytic
R4701 T5289 T5288 arg1Of cells,THP-1
R4702 T5289 T5290 arg1Of cells,were
R4703 T5289 T5292 arg2Of cells,transfected
R4704 T5289 T5302 arg2Of cells,cultured
R4705 T5292 T5293 arg1Of transfected,as
R4706 T5292 T5300 arg1Of transfected,and
R4707 T5295 T5294 arg1Of described,previously
R4708 T5297 T5293 arg2Of 30,as
R4709 T5297 T5295 arg1Of 30,described
R4710 T5297 T5296 arg2Of 30,[
R4711 T5298 T5296 arg3Of ],[
R4712 T5300 T5290 arg2Of and,were
R4713 T5300 T5291 arg1Of and,transiently
R4714 T5300 T5299 arg1Of and,","
R4715 T5302 T5300 arg2Of cultured,and
R4716 T5302 T5301 arg1Of cultured,then
R4717 T5302 T5303 arg1Of cultured,for
R4718 T5305 T5303 arg2Of h,for
R4719 T5305 T5304 arg1Of h,4
R4720 T5305 T5306 arg1Of h,alone
R4721 T5305 T5308 arg1Of h,with
R4722 T5306 T5307 arg1Of alone,or
R4723 T5308 T5307 arg2Of with,or
R4724 T5310 T5308 arg2Of concentration,with
R4725 T5310 T5309 arg1Of concentration,increasing
R4726 T5310 T5311 arg1Of concentration,of
R4727 T5313 T5314 arg1Of C10,or
R4728 T5314 T5311 arg2Of or,of
R4729 T5314 T5312 arg1Of or,either
R4730 T5315 T5314 arg2Of C40,or
R4731 T5317 T5316 arg1Of activity,Luciferase
R4732 T5317 T5318 arg1Of activity,was
R4733 T5317 T5319 arg2Of activity,determined
R4734 T5319 T5318 arg2Of determined,was
R4735 T5320 T5319 arg3Of using,determined
R4736 T5322 T5320 arg2Of luminometer,using
R4737 T5322 T5321 arg1Of luminometer,a
R4738 T5322 T5323 arg1Of luminometer,(
R4739 T5326 T5323 arg2Of Luminometer,(
R4740 T5326 T5324 arg1Of Luminometer,Monolight
R4741 T5326 T5325 arg1Of Luminometer,2010
R4742 T5326 T5327 arg1Of Luminometer,","
R4743 T5329 T5327 arg2Of Arbor,","
R4744 T5329 T5328 arg1Of Arbor,Ann
R4745 T5329 T5330 arg1Of Arbor,","
R4746 T5331 T5330 arg2Of MI,","
R4747 T5332 T5323 arg3Of ),(
R4845 T5493 T5492 arg1Of Blot,Western
R4846 T5494 T5493 arg1Of Analysis,Blot
R4847 T5496 T5495 arg1Of cells,THP-1
R4848 T5496 T5497 arg1Of cells,were
R4849 T5496 T5498 arg2Of cells,stimulated
R4850 T5498 T5497 arg2Of stimulated,were
R4851 T5498 T5499 arg1Of stimulated,for
R4852 T5501 T5499 arg2Of lengths,for
R4853 T5501 T5500 arg1Of lengths,various
R4854 T5501 T5502 arg1Of lengths,of
R4855 T5503 T5502 arg2Of time,of
R4856 T5503 T5504 arg1Of time,with
R4857 T5505 T5506 arg1Of 0.1,mg/ml
R4858 T5507 T5505 arg1Of C10,0.1
R4859 T5507 T5508 arg1Of C10,or
R4860 T5508 T5511 arg1Of or,or
R4861 T5509 T5508 arg2Of C40,or
R4862 T5511 T5504 arg2Of or,with
R4863 T5511 T5510 arg1Of or,","
R4864 T5514 T5511 arg2Of LPS,or
R4865 T5514 T5512 arg1Of LPS,10
R4866 T5514 T5513 arg1Of LPS,µg/ml
R4867 T5515 T5516 arg1Of Cells,were
R4868 T5515 T5518 arg2Of Cells,pelleted
R4869 T5515 T5520 arg2Of Cells,washed
R4870 T5515 T5522 arg2Of Cells,homogenised
R4871 T5518 T5519 arg1Of pelleted,","
R4872 T5519 T5521 arg1Of ",",and
R4873 T5520 T5519 arg2Of washed,","
R4874 T5521 T5516 arg2Of and,were
R4875 T5521 T5517 arg1Of and,then
R4876 T5522 T5521 arg2Of homogenised,and
R4877 T5522 T5523 arg1Of homogenised,in
R4878 T5525 T5523 arg2Of buffer,in
R4879 T5525 T5524 arg1Of buffer,lysis
R4880 T5525 T5526 arg1Of buffer,(
R4881 T5525 T5551 arg1Of buffer,on
R4882 T5529 T5526 arg2Of Hepes,(
R4883 T5529 T5527 arg1Of Hepes,10
R4884 T5529 T5528 arg1Of Hepes,mM
R4885 T5529 T5530 arg1Of Hepes,","
R4886 T5531 T5532 arg1Of pH,7.9
R4887 T5531 T5533 arg1Of pH,","
R4888 T5533 T5537 arg1Of ",",","
R4889 T5534 T5535 arg1Of 150,mM
R4890 T5536 T5533 arg2Of NaCl,","
R4891 T5536 T5534 arg1Of NaCl,150
R4892 T5537 T5541 arg1Of ",",","
R4893 T5538 T5539 arg1Of 1,mM
R4894 T5540 T5537 arg2Of EDTA,","
R4895 T5540 T5538 arg1Of EDTA,1
R4896 T5541 T5546 arg1Of ",",and
R4897 T5542 T5543 arg1Of 0.6,%
R4898 T5544 T5541 arg2Of NP-40,","
R4899 T5544 T5542 arg1Of NP-40,0.6
R4900 T5546 T5530 arg2Of and,","
R4901 T5546 T5545 arg1Of and,","
R4902 T5547 T5548 arg1Of 0.5,mM
R4903 T5549 T5546 arg2Of PMSF,and
R4904 T5549 T5547 arg1Of PMSF,0.5
R4905 T5550 T5526 arg3Of ),(
R4906 T5552 T5551 arg2Of ice,on
R4907 T5553 T5554 arg1Of Homogenates,were
R4908 T5553 T5555 arg2Of Homogenates,sonicated
R4909 T5553 T5557 arg2Of Homogenates,centrifuged
R4910 T5555 T5556 arg1Of sonicated,","
R4911 T5556 T5554 arg2Of ",",were
R4912 T5556 T5561 modOf ",",to
R4913 T5557 T5556 arg2Of centrifuged,","
R4914 T5557 T5558 arg1Of centrifuged,at
R4915 T5560 T5558 arg2Of rpm,at
R4916 T5560 T5559 arg1Of rpm,"10,000"
R4917 T5562 T5561 arg1Of remove,to
R4918 T5564 T5563 arg1Of debris,cellular
R4919 T5564 T5566 arg1Of debris,and
R4920 T5566 T5562 arg2Of and,remove
R4921 T5566 T5565 arg1Of and,","
R4922 T5566 T5568 arg2Of and,collected
R4923 T5567 T5566 arg2Of supernatant,and
R4924 T5570 T5569 arg1Of concentration,Protein
R4925 T5570 T5571 arg1Of concentration,was
R4926 T5570 T5572 arg2Of concentration,determined
R4927 T5572 T5571 arg2Of determined,was
R4928 T5573 T5572 arg3Of using,determined
R4929 T5577 T5573 arg2Of Assay,using
R4930 T5577 T5574 arg1Of Assay,the
R4931 T5577 T5575 arg1Of Assay,DC
R4932 T5577 T5576 arg1Of Assay,Protein
R4933 T5577 T5578 arg1Of Assay,(
R4934 T5579 T5578 arg2Of Bio-Rad,(
R4935 T5580 T5578 arg3Of ),(
R4936 T5581 T5582 arg1Of Proteins,in
R4937 T5581 T5590 arg1Of Proteins,were
R4938 T5581 T5591 arg2Of Proteins,resolved
R4939 T5581 T5600 arg2Of Proteins,transferred
R4940 T5583 T5582 arg2Of samples,in
R4941 T5583 T5584 arg1Of samples,(
R4942 T5588 T5584 arg2Of proteins,(
R4943 T5588 T5585 arg1Of proteins,15
R4944 T5588 T5586 arg1Of proteins,µg
R4945 T5588 T5587 arg1Of proteins,total
R4946 T5589 T5584 arg3Of ),(
R4947 T5591 T5592 arg1Of resolved,in
R4948 T5591 T5599 arg1Of resolved,and
R4949 T5595 T5596 arg1Of 12,%
R4950 T5598 T5592 arg2Of gel,in
R4951 T5598 T5593 arg1Of gel,a
R4952 T5598 T5594 arg1Of gel,denaturing
R4953 T5598 T5595 arg1Of gel,12
R4954 T5598 T5597 arg1Of gel,polyacrylamide
R4955 T5599 T5590 arg2Of and,were
R4956 T5600 T5599 arg2Of transferred,and
R4957 T5600 T5601 arg1Of transferred,to
R4958 T5604 T5601 arg2Of membrane,to
R4959 T5604 T5602 arg1Of membrane,a
R4960 T5604 T5603 arg1Of membrane,nitrocellulose
R4961 T5606 T5605 arg1Of protein,I-κBα
R4962 T5606 T5607 arg1Of protein,was
R4963 T5606 T5608 arg2Of protein,detected
R4964 T5606 T5609 arg1Of protein,using
R4965 T5608 T5607 arg2Of detected,was
R4966 T5609 T5608 arg3Of using,detected
R4967 T5609 T5621 modOf using,followed
R4968 T5613 T5609 arg2Of antibody,using
R4969 T5613 T5610 arg1Of antibody,a
R4970 T5613 T5611 arg1Of antibody,rabbit
R4971 T5613 T5612 arg1Of antibody,polyclonal
R4972 T5613 T5614 arg1Of antibody,(
R4973 T5617 T5614 arg2Of Biotechnology,(
R4974 T5617 T5615 arg1Of Biotechnology,Santa
R4975 T5617 T5616 arg1Of Biotechnology,Cruz
R4976 T5617 T5618 arg1Of Biotechnology,","
R4977 T5619 T5618 arg2Of CA,","
R4978 T5620 T5614 arg3Of ),(
R4979 T5628 T5621 arg1Of antibody,followed
R4980 T5628 T5622 arg2Of antibody,by
R4981 T5628 T5623 arg1Of antibody,a
R4982 T5628 T5624 arg1Of antibody,horseradish
R4983 T5628 T5625 arg1Of antibody,peroxidase-coupled
R4984 T5628 T5626 arg1Of antibody,goat
R4985 T5628 T5627 arg1Of antibody,polyclonal
R4986 T5628 T5629 arg1Of antibody,against
R4987 T5631 T5629 arg2Of Ig,against
R4988 T5631 T5630 arg1Of Ig,rabbit
R4989 T5631 T5632 arg1Of Ig,(
R4990 T5634 T5632 arg2Of Laboratories,(
R4991 T5634 T5633 arg1Of Laboratories,Caltag
R4992 T5635 T5632 arg3Of ),(
R4993 T5639 T5638 arg1Of bands,IκB
R4994 T5639 T5640 arg1Of bands,were
R4995 T5639 T5641 arg2Of bands,revealed
R4996 T5641 T5636 arg1Of revealed,Finally
R4997 T5641 T5637 arg1Of revealed,","
R4998 T5641 T5640 arg2Of revealed,were
R4999 T5641 T5642 modOf revealed,using
R5000 T5641 T5647 arg1Of revealed,(
R5001 T5641 T5657 arg1Of revealed,according
R5002 T5646 T5642 arg2Of system,using
R5003 T5646 T5643 arg1Of system,the
R5004 T5646 T5644 arg1Of system,ECL™
R5005 T5646 T5645 arg1Of system,detection
R5006 T5650 T5647 arg2Of Biotech,(
R5007 T5650 T5648 arg1Of Biotech,Amersham
R5008 T5650 T5649 arg1Of Biotech,Pharmacia
R5009 T5650 T5651 arg1Of Biotech,","
R5010 T5653 T5651 arg2Of Ullis,","
R5011 T5653 T5652 arg1Of Ullis,Les
R5012 T5653 T5654 arg1Of Ullis,","
R5013 T5655 T5654 arg2Of France,","
R5014 T5656 T5647 arg3Of ),(
R5015 T5658 T5657 arg2Of to,according
R5016 T5660 T5659 arg1Of manufacturers,the
R5017 T5660 T5661 arg2Of manufacturers,'
R5018 T5662 T5658 arg2Of instruction,to
R5019 T5662 T5661 arg1Of instruction,'
R5020 T5663 T5664 arg1Of Antibody,to
R5021 T5663 T5670 arg1Of Antibody,was
R5022 T5665 T5664 arg2Of α-Tubulin,to
R5023 T5665 T5666 arg1Of α-Tubulin,(
R5024 T5668 T5666 arg2Of Cruz,(
R5025 T5668 T5667 arg1Of Cruz,Santa
R5026 T5669 T5666 arg3Of ),(
R5027 T5670 T5672 arg1Of was,as
R5028 T5671 T5670 arg2Of use,was
R5029 T5673 T5672 arg2Of loading,as
R5030 T5674 T5673 arg2Of control,loading
R5031 T5677 T5675 arg2Of NF-κB,For
R5032 T5677 T5676 arg1Of NF-κB,nuclear
R5033 T5680 T5679 arg1Of cells,THP-1
R5034 T5680 T5681 arg1Of cells,were
R5035 T5680 T5682 arg2Of cells,stimulated
R5036 T5682 T5681 arg2Of stimulated,were
R5037 T5682 T5683 arg1Of stimulated,with
R5038 T5686 T5687 arg1Of C10,or
R5039 T5687 T5683 arg2Of or,with
R5040 T5687 T5684 arg1Of or,1
R5041 T5687 T5685 arg1Of or,mg/ml
R5042 T5687 T5689 arg1Of or,for
R5043 T5688 T5687 arg2Of C40,or
R5044 T5691 T5690 arg1Of minutes,30
R5045 T5691 T5692 arg1Of minutes,at
R5046 T5691 T5695 arg1Of minutes,were
R5047 T5691 T5697 arg2Of minutes,pelleted
R5048 T5694 T5692 arg2Of Cells,at
R5049 T5694 T5693 arg1Of Cells,37°C.
R5050 T5697 T5675 arg1Of pelleted,For
R5051 T5697 T5678 arg1Of pelleted,","
R5052 T5697 T5681 modOf pelleted,were
R5053 T5697 T5695 arg2Of pelleted,were
R5054 T5697 T5696 arg1Of pelleted,then
R5055 T5697 T5698 arg1Of pelleted,and
R5056 T5699 T5697 arg3Of nuclei,pelleted
R5057 T5699 T5700 arg2Of nuclei,separated
R5058 T5700 T5701 arg1Of separated,as
R5059 T5702 T5701 arg2Of described,as
R5060 T5702 T5703 arg1Of described,[
R5061 T5704 T5703 arg2Of 31,[
R5062 T5705 T5703 arg3Of ],[
R5063 T5706 T5707 arg1Of Nuclei,were
R5064 T5706 T5708 arg2Of Nuclei,washed
R5065 T5706 T5710 arg2Of Nuclei,homogenized
R5066 T5706 T5719 arg2Of Nuclei,heated
R5067 T5708 T5709 arg1Of washed,and
R5068 T5709 T5707 arg2Of and,were
R5069 T5709 T5726 arg1Of and,in
R5070 T5710 T5711 arg1Of homogenized,directly
R5071 T5710 T5712 arg1Of homogenized,in
R5072 T5710 T5718 arg1Of homogenized,and
R5073 T5715 T5714 arg2Of Laemli,(
R5074 T5716 T5714 arg3Of ),(
R5075 T5717 T5712 arg2Of buffer,in
R5076 T5717 T5713 arg1Of buffer,loading
R5077 T5717 T5714 arg1Of buffer,(
R5078 T5718 T5709 arg2Of and,and
R5079 T5719 T5718 arg2Of heated,and
R5080 T5719 T5720 arg1Of heated,for
R5081 T5722 T5720 arg2Of minutes,for
R5082 T5722 T5721 arg1Of minutes,5
R5083 T5722 T5723 arg1Of minutes,at
R5084 T5725 T5723 arg2Of Proteins,at
R5085 T5725 T5724 arg1Of Proteins,100°C.
R5086 T5727 T5728 arg1Of samples,were
R5087 T5727 T5729 arg2Of samples,resolved
R5088 T5727 T5738 arg2Of samples,transferred
R5089 T5729 T5730 arg1Of resolved,in
R5090 T5729 T5737 arg1Of resolved,and
R5091 T5733 T5734 arg1Of 8,%
R5092 T5736 T5730 arg2Of gel,in
R5093 T5736 T5731 arg1Of gel,a
R5094 T5736 T5732 arg1Of gel,denaturing
R5095 T5736 T5733 arg1Of gel,8
R5096 T5736 T5735 arg1Of gel,polyacrylamide
R5097 T5737 T5726 arg2Of and,in
R5098 T5737 T5728 arg2Of and,were
R5099 T5738 T5737 arg2Of transferred,and
R5100 T5738 T5739 arg1Of transferred,to
R5101 T5744 T5743 arg2Of PVDF,(
R5102 T5745 T5743 arg3Of ),(
R5103 T5746 T5739 arg2Of membrane,to
R5104 T5746 T5740 arg1Of membrane,a
R5105 T5746 T5741 arg1Of membrane,polyvinylidine
R5106 T5746 T5742 arg1Of membrane,fluoride
R5107 T5746 T5743 arg1Of membrane,(
R5108 T5746 T5747 arg1Of membrane,(
R5109 T5748 T5747 arg2Of Immobilon-P,(
R5110 T5748 T5749 arg1Of Immobilon-P,;
R5111 T5748 T5753 arg1Of Immobilon-P,","
R5112 T5748 T5754 arg1Of Immobilon-P,MA
R5113 T5750 T5751 arg1Of Millipore,","
R5114 T5751 T5749 arg2Of ",",;
R5115 T5752 T5751 arg2Of Bedford,","
R5116 T5755 T5747 arg3Of ),(
R5117 T5756 T5757 arg1Of Membranes,were
R5118 T5756 T5758 arg2Of Membranes,incubated
R5119 T5758 T5757 arg2Of incubated,were
R5120 T5758 T5759 arg1Of incubated,in
R5121 T5760 T5759 arg2Of blocking,in
R5122 T5761 T5760 arg2Of buffer,blocking
R5123 T5761 T5762 arg1Of buffer,(
R5124 T5761 T5770 arg1Of buffer,for
R5125 T5763 T5764 arg1Of 1,%
R5126 T5765 T5762 arg2Of BSA,(
R5127 T5765 T5763 arg1Of BSA,1
R5128 T5765 T5766 arg1Of BSA,","
R5129 T5765 T5767 arg1Of BSA,in
R5130 T5768 T5767 arg2Of PBS,in
R5131 T5769 T5762 arg3Of ),(
R5132 T5772 T5770 arg2Of hours,for
R5133 T5772 T5771 arg1Of hours,two
R5134 T5772 T5773 arg1Of hours,at
R5135 T5775 T5773 arg2Of temperature,at
R5136 T5775 T5774 arg1Of temperature,room
R5137 T5776 T5777 arg1Of Membranes,were
R5138 T5776 T5779 arg2Of Membranes,probed
R5139 T5779 T5777 arg2Of probed,were
R5140 T5779 T5778 arg1Of probed,subsequently
R5141 T5779 T5780 arg1Of probed,with
R5142 T5779 T5787 arg1Of probed,","
R5143 T5779 T5788 arg1Of probed,overnight
R5144 T5783 T5780 arg2Of antibody,with
R5145 T5783 T5781 arg1Of antibody,the
R5146 T5783 T5782 arg1Of antibody,corresponding
R5147 T5783 T5784 arg1Of antibody,in
R5148 T5785 T5784 arg2Of blocking,in
R5149 T5786 T5785 arg2Of buffer,blocking
R5150 T5794 T5789 arg1Of subunit,Rabbit
R5151 T5794 T5790 arg1Of subunit,polyclonal
R5152 T5794 T5791 arg1Of subunit,antibody
R5153 T5794 T5792 arg1Of subunit,anti-NF-κB
R5154 T5794 T5793 arg1Of subunit,p50
R5155 T5794 T5795 arg1Of subunit,(
R5156 T5794 T5799 arg1Of subunit,or
R5157 T5797 T5795 arg2Of sc-114,(
R5158 T5797 T5796 arg1Of sc-114,#
R5159 T5798 T5795 arg3Of ),(
R5160 T5799 T5807 arg1Of or,from
R5161 T5799 T5811 arg1Of or,were
R5162 T5799 T5812 arg2Of or,used
R5163 T5802 T5799 arg2Of subunit,or
R5164 T5802 T5800 arg1Of subunit,anti-NF-κB
R5165 T5802 T5801 arg1Of subunit,p65
R5166 T5802 T5803 arg1Of subunit,(
R5167 T5805 T5803 arg2Of sc-109,(
R5168 T5805 T5804 arg1Of sc-109,#
R5169 T5806 T5803 arg3Of ),(
R5170 T5810 T5807 arg2Of Biotechnology,from
R5171 T5810 T5808 arg1Of Biotechnology,Santa
R5172 T5810 T5809 arg1Of Biotechnology,Cruz
R5173 T5812 T5811 arg2Of used,were
R5174 T5813 T5814 arg1Of Membranes,were
R5175 T5813 T5815 arg2Of Membranes,washed
R5176 T5813 T5832 arg2Of Membranes,incubated
R5177 T5815 T5817 arg1Of washed,times
R5178 T5815 T5818 arg1Of washed,in
R5179 T5815 T5820 arg1Of washed,with
R5180 T5815 T5824 arg1Of washed,20
R5181 T5815 T5827 arg1Of washed,minutes
R5182 T5815 T5829 arg1Of washed,time
R5183 T5815 T5831 arg1Of washed,and
R5184 T5817 T5816 arg1Of times,six
R5185 T5819 T5818 arg2Of PBS,in
R5186 T5822 T5820 arg2Of %,with
R5187 T5822 T5821 arg1Of %,0.05
R5188 T5824 T5823 arg1Of 20,Tween
R5189 T5824 T5825 arg1Of 20,","
R5190 T5827 T5825 arg2Of minutes,","
R5191 T5827 T5826 arg1Of minutes,5
R5192 T5829 T5828 arg1Of time,each
R5193 T5831 T5814 arg2Of and,were
R5194 T5831 T5830 arg1Of and,","
R5195 T5832 T5831 arg2Of incubated,and
R5196 T5832 T5833 arg1Of incubated,with
R5197 T5832 T5861 arg1Of incubated,for
R5198 T5833 T5859 arg1Of with,in
R5199 T5836 T5834 arg1Of dilution,a
R5200 T5836 T5835 arg1Of dilution,1/3000
R5201 T5836 T5837 arg1Of dilution,of
R5202 T5839 T5837 arg2Of F,of
R5203 T5839 T5838 arg1Of F,HRP-conjugated
R5204 T5841 T5840 arg2Of ab,(
R5205 T5841 T5842 arg1Of ab,'
R5206 T5843 T5840 arg3Of ),(
R5207 T5847 T5833 arg2Of IgG,with
R5208 T5847 T5836 arg1Of IgG,dilution
R5209 T5847 T5840 arg1Of IgG,(
R5210 T5847 T5844 arg1Of IgG,2
R5211 T5847 T5845 arg1Of IgG,goat
R5212 T5847 T5846 arg1Of IgG,anti-rabbit
R5213 T5847 T5848 arg1Of IgG,in
R5214 T5849 T5850 arg1Of 5,%
R5215 T5853 T5849 arg1Of milk,5
R5216 T5853 T5851 arg1Of milk,nonfat
R5217 T5853 T5852 arg1Of milk,dry
R5218 T5853 T5854 arg1Of milk,and
R5219 T5854 T5848 arg2Of and,in
R5220 T5856 T5855 arg1Of %,0.05
R5221 T5858 T5854 arg2Of 20,and
R5222 T5858 T5856 arg1Of 20,%
R5223 T5858 T5857 arg1Of 20,Tween
R5224 T5860 T5859 arg2Of PBS,in
R5225 T5863 T5861 arg2Of hour,for
R5226 T5863 T5862 arg1Of hour,1
R5227 T5863 T5864 arg1Of hour,at
R5228 T5866 T5864 arg2Of temperature,at
R5229 T5866 T5865 arg1Of temperature,room
R5230 T5868 T5867 arg2Of washing,After
R5231 T5868 T5872 arg1Of washing,in
R5232 T5868 T5874 arg1Of washing,with
R5233 T5870 T5869 arg1Of more,six
R5234 T5871 T5868 arg2Of times,washing
R5235 T5871 T5870 arg1Of times,more
R5236 T5873 T5872 arg2Of PBS,in
R5237 T5876 T5875 arg1Of %,0.05
R5238 T5878 T5874 arg2Of 20,with
R5239 T5878 T5876 arg1Of 20,%
R5240 T5878 T5877 arg1Of 20,Tween
R5241 T5881 T5868 arg1Of proteins,washing
R5242 T5881 T5880 arg1Of proteins,antibody-reactive
R5243 T5881 T5882 arg1Of proteins,were
R5244 T5881 T5883 arg2Of proteins,detected
R5245 T5883 T5867 arg1Of detected,After
R5246 T5883 T5879 arg1Of detected,","
R5247 T5883 T5882 arg2Of detected,were
R5248 T5884 T5883 arg3Of using,detected
R5249 T5884 T5888 arg1Of using,(
R5250 T5884 T5897 arg1Of using,according
R5251 T5887 T5884 arg2Of substrate,using
R5252 T5887 T5885 arg1Of substrate,a
R5253 T5887 T5886 arg1Of substrate,chemiluminescence
R5254 T5889 T5890 arg1Of SuperSignal,;
R5255 T5890 T5888 arg2Of ;,(
R5256 T5890 T5894 arg1Of ;,","
R5257 T5891 T5892 arg1Of Pierce,","
R5258 T5892 T5890 arg2Of ",",;
R5259 T5893 T5892 arg2Of Rockford,","
R5260 T5895 T5894 arg2Of IL,","
R5261 T5896 T5888 arg3Of ),(
R5262 T5898 T5897 arg2Of to,according
R5263 T5900 T5899 arg1Of manufacturer,the
R5264 T5900 T5901 arg2Of manufacturer,'s
R5265 T5902 T5898 arg2Of instructions,to
R5266 T5902 T5901 arg1Of instructions,'s
R5267 T5904 T5903 arg1Of confirm,To
R5268 T5907 T5906 arg1Of amounts,equivalent
R5269 T5907 T5908 arg1Of amounts,of
R5270 T5907 T5910 arg1Of amounts,were
R5271 T5907 T5911 arg2Of amounts,loaded
R5272 T5909 T5908 arg2Of protein,of
R5273 T5911 T5904 arg2Of loaded,confirm
R5274 T5911 T5905 arg1Of loaded,that
R5275 T5911 T5910 arg2Of loaded,were
R5276 T5911 T5912 arg1Of loaded,in
R5277 T5914 T5912 arg2Of line,in
R5278 T5914 T5913 arg1Of line,each
R5279 T5916 T5904 arg1Of membranes,confirm
R5280 T5916 T5917 arg1Of membranes,were
R5281 T5916 T5920 arg1Of membranes,blotted
R5282 T5917 T5903 modOf were,To
R5283 T5917 T5915 arg1Of were,","
R5284 T5917 T5918 arg1Of were,also
R5285 T5917 T5923 arg1Of were,as
R5286 T5920 T5917 arg2Of blotted,were
R5287 T5920 T5919 arg1Of blotted,Western
R5288 T5920 T5921 arg1Of blotted,for
R5289 T5922 T5921 arg2Of ERK,for
R5290 T5924 T5923 arg2Of described,as
R5291 T5924 T5925 arg1Of described,[
R5292 T5926 T5925 arg2Of 32,[
R5293 T5927 T5925 arg3Of ],[
R5753 T6465 T6466 arg1Of Analysis,of
R5754 T6465 T6469 arg1Of Analysis,by
R5755 T6468 T6466 arg2Of Activation,of
R5756 T6468 T6467 arg1Of Activation,NF-κB
R5757 T6471 T6469 arg2Of Cytometry,by
R5758 T6471 T6470 arg1Of Cytometry,Flow
R5759 T6473 T6472 arg1Of activation,Nuclear
R5760 T6473 T6474 arg1Of activation,of
R5761 T6473 T6476 arg1Of activation,by
R5762 T6473 T6479 arg1Of activation,was
R5763 T6473 T6480 arg2Of activation,performed
R5764 T6475 T6474 arg2Of NF−κΒ,of
R5765 T6478 T6476 arg2Of cytometry,by
R5766 T6478 T6477 arg1Of cytometry,flow
R5767 T6480 T6479 arg2Of performed,was
R5768 T6480 T6481 arg1Of performed,as
R5769 T6482 T6481 arg2Of described,as
R5770 T6482 T6483 arg1Of described,[
R5771 T6484 T6483 arg2Of 31,[
R5772 T6485 T6483 arg3Of ],[
R5800 T6521 T6520 arg1Of Analysis,Statistical
R5801 T6523 T6522 arg1Of results,The
R5802 T6523 T6524 arg1Of results,were
R5803 T6523 T6525 arg2Of results,expressed
R5804 T6525 T6524 arg2Of expressed,were
R5805 T6525 T6526 arg1Of expressed,as
R5806 T6529 T6527 arg1Of value,the
R5807 T6529 T6528 arg1Of value,mean
R5808 T6529 T6530 arg1Of value,±
R5809 T6530 T6526 arg2Of ±,as
R5810 T6531 T6530 arg2Of S.E.M.,±
R5811 T6531 T6532 arg1Of S.E.M.,of
R5812 T6534 T6532 arg2Of experiments,of
R5813 T6534 T6533 arg1Of experiments,individual
R5814 T6537 T6535 arg1Of significance,The
R5815 T6537 T6536 arg1Of significance,statistical
R5816 T6537 T6538 arg1Of significance,of
R5817 T6537 T6544 arg1Of significance,was
R5818 T6537 T6545 arg2Of significance,assessed
R5819 T6540 T6538 arg2Of differences,of
R5820 T6540 T6539 arg1Of differences,the
R5821 T6540 T6541 arg1Of differences,between
R5822 T6543 T6541 arg2Of values,between
R5823 T6543 T6542 arg1Of values,mean
R5824 T6545 T6544 arg2Of assessed,was
R5825 T6550 T6551 arg1Of t-test,and
R5826 T6551 T6545 arg1Of and,assessed
R5827 T6551 T6546 arg2Of and,by
R5828 T6551 T6547 arg1Of and,the
R5829 T6551 T6548 arg1Of and,Student
R5830 T6551 T6549 arg1Of and,'s
R5831 T6551 T6553 arg1Of and,of
R5832 T6552 T6551 arg2Of analysis,and
R5833 T6554 T6553 arg2Of variance,of
R5834 T6554 T6555 arg1Of variance,(
R5835 T6556 T6555 arg2Of ANOVA,(
R5836 T6557 T6555 arg3Of ),(
R1782 T2071 T2070 arg2Of carrageenan,degraded
R1783 T2071 T2072 arg1Of carrageenan,with
R1785 T2073 T2074 arg1Of low,","
R1793 T2082 T2072 arg2Of and,with

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T2416 23-34 NNP denotes Preparation
T2417 35-37 IN denotes of
T2418 38-46 NNP denotes Degraded
T2419 47-58 NNP denotes Carrageenan
T2420 59-62 CD denotes Two
T2421 63-75 NNS denotes preparations
T2422 76-78 IN denotes of
T2423 79-87 JJ denotes degraded
T2424 88-99 NN denotes carrageenan
T2425 100-104 IN denotes with
T2426 105-108 JJ denotes low
T2427 108-109 , denotes ,
T2428 110-111 -LRB- denotes (
T2429 111-112 FW denotes ∼
T2430 112-114 CD denotes 10
T2431 115-118 NN denotes kDa
T2432 118-119 : denotes ;
T2433 120-123 NNP denotes C10
T2434 123-124 -RRB- denotes )
T2435 124-125 , denotes ,
T2436 126-129 CC denotes and
T2437 130-136 NN denotes medium
T2438 136-137 , denotes ,
T2439 138-139 -LRB- denotes (
T2440 139-140 FW denotes ∼
T2441 140-142 CD denotes 40
T2442 143-146 NN denotes kDa
T2443 146-147 : denotes ;
T2444 148-151 NNP denotes C40
T2445 151-152 -RRB- denotes )
T2446 153-162 JJ denotes molecular
T2447 163-169 NN denotes weight
T2448 170-174 VBD denotes were
T2449 175-183 VBN denotes prepared
T2450 184-188 IN denotes from
T2451 189-195 JJ denotes native
T2452 196-212 JJ denotes iota-carrageenan
T2453 213-222 VBN denotes extracted
T2454 223-227 IN denotes from
T2455 228-235 NNP denotes Euchema
T2456 236-244 NN denotes spinosum
T2457 245-246 -LRB- denotes (
T2458 246-256 RB denotes generously
T2459 257-265 VBN denotes provided
T2460 266-268 IN denotes by
T2461 269-275 NNP denotes Sanofi
T2462 276-286 NNPS denotes Biosystems
T2463 287-295 NNP denotes Industry
T2464 295-296 , denotes ,
T2465 297-317 NNP denotes Boulogne-Billancourt
T2466 317-318 , denotes ,
T2467 319-325 NNP denotes France
T2468 325-326 -RRB- denotes )
T2469 326-327 . denotes .
T2470 328-334 JJ denotes Native
T2471 335-346 NN denotes carrageenan
T2472 347-350 VBD denotes was
T2473 351-360 VBN denotes dissolved
T2474 361-363 IN denotes in
T2475 364-373 JJ denotes distilled
T2476 374-379 NN denotes water
T2477 380-381 -LRB- denotes (
T2478 381-382 CD denotes 5
T2479 382-383 NN denotes %
T2480 384-387 NN denotes w/v
T2481 387-388 -RRB- denotes )
T2482 389-394 IN denotes under
T2483 395-403 JJ denotes vigorous
T2484 404-412 VBG denotes stirring
T2485 413-416 CC denotes and
T2486 417-423 VBN denotes heated
T2487 424-426 TO denotes to
T2488 427-429 CD denotes 60
T2489 429-430 CD denotes °
T2490 430-432 NNP denotes C.
T2491 433-437 RB denotes Then
T2492 437-438 , denotes ,
T2493 439-442 DT denotes the
T2494 443-454 NN denotes carrageenan
T2495 455-463 NN denotes solution
T2496 464-467 VBD denotes was
T2497 468-477 VBN denotes submitted
T2498 478-480 TO denotes to
T2499 481-484 CD denotes two
T2500 485-494 JJ denotes different
T2501 495-505 NNS denotes treatments
T2502 506-508 TO denotes to
T2503 509-515 VB denotes obtain
T2504 516-520 DT denotes both
T2505 521-524 JJ denotes low
T2506 525-528 CC denotes and
T2507 529-535 JJ denotes medium
T2508 536-545 JJ denotes molecular
T2509 546-552 NN denotes weight
T2510 553-562 NNS denotes fractions
T2511 562-563 . denotes .
T2512 564-571 RB denotes Briefly
T2513 571-572 , denotes ,
T2514 573-576 IN denotes for
T2515 577-580 DT denotes the
T2516 581-584 JJ denotes low
T2517 585-594 JJ denotes molecular
T2518 595-601 NN denotes weight
T2519 602-610 NN denotes fraction
T2520 610-611 , denotes ,
T2521 612-623 NN denotes carrageenan
T2522 624-632 NN denotes solution
T2523 633-636 VBD denotes was
T2524 637-647 VBN denotes hydrolyzed
T2525 648-652 IN denotes with
T2526 653-656 CD denotes 0.3
T2527 656-657 NN denotes %
T2528 658-659 -LRB- denotes (
T2529 659-662 FW denotes v/v
T2530 662-663 -RRB- denotes )
T2531 664-676 VBD denotes concentrated
T2532 677-686 JJ denotes sulphuric
T2533 687-691 NN denotes acid
T2534 692-695 IN denotes for
T2535 696-698 CD denotes 15
T2536 699-702 NN denotes min
T2537 703-705 IN denotes at
T2538 706-708 CD denotes 80
T2539 708-709 CD denotes °
T2540 709-710 NNP denotes C
T2541 710-711 . denotes .
T2542 712-717 IN denotes After
T2543 718-732 NN denotes neutralization
T2544 733-737 IN denotes with
T2545 738-742 NNP denotes NaOH
T2546 743-745 NNP denotes 4N
T2547 745-746 , denotes ,
T2548 747-750 DT denotes the
T2549 751-759 NN denotes solution
T2550 760-763 VBD denotes was
T2551 764-769 JJ denotes ultra
T2552 770-778 VBN denotes filtered
T2553 779-786 IN denotes through
T2554 787-788 DT denotes a
T2555 789-795 JJ denotes hollow
T2556 796-801 NN denotes fibre
T2557 802-811 NN denotes cartridge
T2558 812-816 IN denotes with
T2559 817-819 NNP denotes MW
T2560 820-827 NN denotes cut-off
T2561 828-829 CD denotes 5
T2562 830-833 NNP denotes kDa
T2563 833-834 , denotes ,
T2564 835-836 -LRB- denotes (
T2565 836-842 NNP denotes Amicon
T2566 843-846 NNP denotes Inc
T2567 846-847 , denotes ,
T2568 848-855 NNP denotes Beverly
T2569 855-856 , denotes ,
T2570 857-860 NNP denotes USA
T2571 860-861 -RRB- denotes )
T2572 861-862 . denotes .
T2573 863-866 IN denotes For
T2574 867-870 DT denotes the
T2575 871-877 NN denotes medium
T2576 878-887 JJ denotes molecular
T2577 888-894 NN denotes weight
T2578 895-903 NN denotes fraction
T2579 903-904 , denotes ,
T2580 905-908 DT denotes the
T2581 909-920 NN denotes carrageenan
T2582 921-929 NN denotes solution
T2583 930-933 VBD denotes was
T2584 934-944 VBN denotes hydrolyzed
T2585 945-949 IN denotes with
T2586 950-953 CD denotes 0.3
T2587 953-954 NN denotes %
T2588 955-956 -LRB- denotes (
T2589 956-959 FW denotes v/v
T2590 959-960 -RRB- denotes )
T2591 961-973 VBD denotes concentrated
T2592 974-983 JJ denotes sulphuric
T2593 984-988 NN denotes acid
T2594 989-992 IN denotes for
T2595 993-995 CD denotes 30
T2596 996-999 NN denotes min
T2597 1000-1002 IN denotes at
T2598 1003-1005 CD denotes 60
T2599 1005-1006 CD denotes °
T2600 1006-1007 NNP denotes C
T2601 1007-1008 . denotes .
T2602 1009-1014 IN denotes After
T2603 1015-1029 NN denotes neutralization
T2604 1029-1030 , denotes ,
T2605 1031-1034 DT denotes the
T2606 1035-1046 NN denotes supernatant
T2607 1047-1050 VBD denotes was
T2608 1051-1056 JJ denotes ultra
T2609 1057-1065 VBN denotes filtered
T2610 1066-1067 -LRB- denotes (
T2611 1067-1069 NNP denotes MW
T2612 1070-1077 NN denotes cut-off
T2613 1078-1081 CD denotes 100
T2614 1082-1085 NNP denotes kDa
T2615 1085-1086 -RRB- denotes )
T2616 1086-1087 . denotes .
T2617 1088-1091 DT denotes The
T2618 1092-1100 NN denotes filtrate
T2619 1101-1104 VBD denotes was
T2620 1105-1114 VBN denotes submitted
T2621 1115-1117 TO denotes to
T2622 1118-1119 DT denotes a
T2623 1120-1126 JJ denotes second
T2624 1127-1132 NN denotes ultra
T2625 1133-1143 NN denotes filtration
T2626 1144-1145 -LRB- denotes (
T2627 1145-1147 NNP denotes MW
T2628 1148-1155 NN denotes cut-off
T2629 1156-1157 CD denotes 5
T2630 1158-1161 NNP denotes kDa
T2631 1161-1162 -RRB- denotes )
T2632 1162-1163 . denotes .
T2633 1164-1168 DT denotes Both
T2634 1169-1181 NNS denotes preparations
T2635 1182-1184 IN denotes of
T2636 1185-1189 NNP denotes dCGN
T2637 1190-1194 VBD denotes were
T2638 1195-1207 VBN denotes precipitated
T2639 1208-1212 IN denotes with
T2640 1213-1214 CD denotes 4
T2641 1215-1222 NNS denotes volumes
T2642 1223-1225 IN denotes of
T2643 1226-1228 CD denotes 95
T2644 1228-1229 NN denotes %
T2645 1230-1237 NN denotes ethanol
T2646 1237-1238 , denotes ,
T2647 1239-1244 VBD denotes dried
T2648 1245-1247 IN denotes at
T2649 1248-1252 NN denotes room
T2650 1253-1264 NN denotes temperature
T2651 1265-1268 CC denotes and
T2652 1269-1275 NN denotes ground
T2653 1276-1278 TO denotes to
T2654 1279-1284 JJ denotes small
T2655 1285-1294 NNS denotes particles
T2656 1295-1296 -LRB- denotes (
T2657 1296-1297 CD denotes 1
T2658 1298-1300 NN denotes mm
T2659 1301-1303 IN denotes in
T2660 1304-1312 NN denotes diameter
T2661 1312-1313 -RRB- denotes )
T2662 1313-1314 . denotes .
T2663 1315-1320 VBG denotes Using
T2664 1321-1335 NN denotes gel-permeation
T2665 1336-1350 NN denotes chromatography
T2666 1351-1353 IN denotes in
T2667 1354-1365 NN denotes combination
T2668 1366-1370 IN denotes with
T2669 1371-1376 JJ denotes light
T2670 1377-1387 VBG denotes scattering
T2671 1388-1400 NNS denotes measurements
T2672 1401-1402 -LRB- denotes (
T2673 1402-1405 VB denotes see
T2674 1406-1412 NNP denotes Viebke
T2675 1413-1415 FW denotes et
T2676 1416-1419 FW denotes al.
T2677 1420-1421 NNP denotes [
T2678 1421-1423 CD denotes 21
T2679 1423-1424 NNP denotes ]
T2680 1424-1425 -RRB- denotes )
T2681 1425-1426 , denotes ,
T2682 1427-1429 PRP denotes it
T2683 1430-1433 VBD denotes was
T2684 1434-1443 VBN denotes confirmed
T2685 1444-1448 IN denotes that
T2686 1449-1452 DT denotes the
T2687 1453-1456 JJ denotes low
T2688 1457-1465 NN denotes fraction
T2689 1466-1469 VBD denotes had
T2690 1470-1472 DT denotes an
T2691 1473-1480 JJ denotes average
T2692 1481-1490 JJ denotes molecular
T2693 1491-1497 NN denotes weight
T2694 1498-1500 IN denotes of
T2695 1501-1503 CD denotes 10
T2696 1504-1507 NN denotes kDa
T2697 1507-1508 , denotes ,
T2698 1509-1512 CC denotes and
T2699 1513-1516 DT denotes the
T2700 1517-1523 NN denotes medium
T2701 1524-1532 NN denotes fraction
T2702 1533-1535 IN denotes of
T2703 1536-1538 CD denotes 40
T2704 1539-1542 NN denotes kDa
T2705 1542-1543 . denotes .
T2706 1544-1547 DT denotes The
T2707 1548-1556 NN denotes sulphate
T2708 1557-1564 NN denotes content
T2709 1565-1567 IN denotes of
T2710 1568-1583 NNS denotes polysaccharides
T2711 1584-1586 IN denotes in
T2712 1587-1591 DT denotes both
T2713 1592-1601 NNS denotes fractions
T2714 1602-1605 VBD denotes was
T2715 1606-1614 VBN denotes measured
T2716 1615-1624 VBG denotes following
T2717 1625-1628 DT denotes the
T2718 1629-1635 NN denotes method
T2719 1636-1638 IN denotes of
T2720 1639-1647 NNP denotes Quemener
T2721 1648-1650 FW denotes et
T2722 1651-1654 FW denotes al.
T2723 1655-1656 NNP denotes [
T2724 1656-1658 CD denotes 22
T2725 1658-1659 NNP denotes ]
T2726 1659-1660 . denotes .
T2727 1661-1668 RB denotes Finally
T2728 1668-1669 , denotes ,
T2729 1670-1673 DT denotes the
T2730 1674-1681 NN denotes absence
T2731 1682-1684 IN denotes of
T2732 1685-1699 NN denotes polysaccharide
T2733 1700-1709 NN denotes structure
T2734 1710-1723 NNS denotes modifications
T2735 1724-1726 IN denotes in
T2736 1727-1730 DT denotes the
T2737 1731-1734 CD denotes two
T2738 1735-1744 NNS denotes fractions
T2739 1745-1748 VBD denotes was
T2740 1749-1758 VBN denotes confirmed
T2741 1759-1764 VBG denotes using
T2742 1765-1771 JJ denotes 2H-NMR
T2743 1772-1784 NN denotes spectroscopy
T2744 1784-1785 . denotes .
T2745 1786-1789 DT denotes The
T2746 1790-1797 NN denotes absence
T2747 1798-1800 IN denotes of
T2748 1801-1804 NNP denotes LPS
T2749 1805-1818 NN denotes contamination
T2750 1819-1821 IN denotes in
T2751 1822-1825 DT denotes the
T2752 1826-1829 CD denotes two
T2753 1830-1839 NNS denotes fractions
T2754 1840-1843 VBD denotes was
T2755 1844-1853 VBN denotes confirmed
T2756 1854-1859 VBG denotes using
T2757 1860-1863 DT denotes the
T2758 1864-1872 JJ denotes e-Toxate
T2759 1872-1873 NN denotes ®
T2760 1874-1877 NN denotes kit
T2761 1878-1879 -LRB- denotes (
T2762 1879-1884 NNP denotes Sigma
T2763 1884-1885 , denotes ,
T2764 1886-1888 NNP denotes St
T2765 1889-1896 NNP denotes Quentin
T2766 1897-1906 NNP denotes Fallavier
T2767 1906-1907 , denotes ,
T2768 1908-1914 NNP denotes France
T2769 1914-1915 -RRB- denotes )
T2770 1915-1916 . denotes .
T2771 1917-1923 IN denotes Before
T2772 1924-1927 NN denotes use
T2773 1928-1930 IN denotes in
T2774 1931-1935 NN denotes cell
T2775 1936-1943 NN denotes culture
T2776 1943-1944 , denotes ,
T2777 1945-1948 DT denotes the
T2778 1949-1952 CD denotes two
T2779 1953-1962 NNS denotes fractions
T2780 1963-1967 VBD denotes were
T2781 1968-1977 VBN denotes dissolved
T2782 1978-1980 IN denotes in
T2783 1981-1989 JJ denotes complete
T2784 1990-1996 NN denotes medium
T2785 1997-2003 IN denotes during
T2786 2004-2006 CD denotes 30
T2787 2007-2010 NN denotes min
T2788 2011-2013 IN denotes at
T2789 2014-2016 CD denotes 56
T2790 2016-2017 CD denotes °
T2791 2017-2019 NNP denotes C.
T2945 2021-2028 NNS denotes Animals
T2946 2028-2029 , denotes ,
T2947 2030-2039 NNPS denotes Chemicals
T2948 2040-2043 CC denotes and
T2949 2044-2048 NNP denotes Diet
T2950 2049-2053 NNP denotes Male
T2951 2054-2060 NNP denotes Wistar
T2952 2061-2065 NNS denotes rats
T2953 2066-2067 -LRB- denotes (
T2954 2067-2070 CD denotes 150
T2955 2071-2072 JJ denotes g
T2956 2073-2080 JJ denotes average
T2957 2081-2087 NN denotes weight
T2958 2087-2088 -RRB- denotes )
T2959 2089-2093 VBD denotes were
T2960 2094-2100 VBN denotes housed
T2961 2101-2106 IN denotes under
T2962 2107-2115 JJ denotes standard
T2963 2116-2126 NNS denotes conditions
T2964 2127-2130 CC denotes and
T2965 2131-2134 VBN denotes fed
T2966 2135-2137 NN denotes ad
T2967 2138-2145 NN denotes libitum
T2968 2146-2150 IN denotes with
T2969 2151-2159 JJ denotes standard
T2970 2160-2166 NN denotes rodent
T2971 2167-2177 NN denotes laboratory
T2972 2178-2182 NN denotes chow
T2973 2182-2183 . denotes .
T2974 2184-2192 JJ denotes Degraded
T2975 2193-2210 NNS denotes iota-carrageenans
T2976 2211-2215 VBD denotes were
T2977 2216-2228 VBN denotes administered
T2978 2229-2231 IN denotes in
T2979 2232-2235 DT denotes the
T2980 2236-2244 NN denotes drinking
T2981 2245-2250 NN denotes water
T2982 2251-2252 -LRB- denotes (
T2983 2252-2253 CD denotes 5
T2984 2253-2254 NN denotes %
T2985 2255-2258 NN denotes w/v
T2986 2258-2259 -RRB- denotes )
T2987 2260-2263 IN denotes for
T2988 2264-2266 CD denotes 55
T2989 2267-2271 NNS denotes days
T2990 2272-2274 TO denotes to
T2991 2275-2276 CD denotes 2
T2992 2277-2283 NNS denotes groups
T2993 2284-2286 IN denotes of
T2994 2287-2290 CD denotes six
T2995 2291-2298 NNS denotes animals
T2996 2299-2303 DT denotes each
T2997 2303-2304 . denotes .
T2998 2305-2308 DT denotes The
T2999 2309-2314 JJ denotes first
T3000 2315-2320 NN denotes group
T3001 2321-2329 VBD denotes received
T3002 2330-2333 DT denotes the
T3003 2334-2337 JJ denotes low
T3004 2338-2347 JJ denotes molecular
T3005 2348-2354 NN denotes weight
T3006 2355-2366 NN denotes carrageenan
T3007 2367-2368 -LRB- denotes (
T3008 2368-2370 CD denotes 10
T3009 2371-2374 NNP denotes kDa
T3010 2375-2379 NNP denotes dCGN
T3011 2379-2380 -RRB- denotes )
T3012 2381-2384 CC denotes and
T3013 2385-2388 DT denotes the
T3014 2389-2395 JJ denotes second
T3015 2396-2404 VBD denotes received
T3016 2405-2408 DT denotes the
T3017 2409-2415 NN denotes medium
T3018 2416-2425 JJ denotes molecular
T3019 2426-2432 NN denotes weight
T3020 2433-2444 NN denotes carrageenan
T3021 2445-2446 -LRB- denotes (
T3022 2446-2448 CD denotes 40
T3023 2449-2452 NNP denotes kDa
T3024 2453-2457 NNP denotes dCGN
T3025 2457-2458 -RRB- denotes )
T3026 2458-2459 . denotes .
T3027 2460-2462 DT denotes An
T3028 2463-2473 JJ denotes additional
T3029 2474-2479 NN denotes group
T3030 2480-2482 IN denotes of
T3031 2483-2487 CD denotes four
T3032 2488-2492 NNS denotes rats
T3033 2493-2497 VBD denotes were
T3034 2498-2508 VBN denotes maintained
T3035 2509-2511 IN denotes on
T3036 2512-2519 JJ denotes regular
T3037 2520-2523 VBP denotes tap
T3038 2524-2529 NN denotes water
T3039 2530-2531 -LRB- denotes (
T3040 2531-2538 NN denotes control
T3041 2539-2544 NN denotes group
T3042 2544-2545 -RRB- denotes )
T3043 2545-2546 . denotes .
T3044 2547-2549 TO denotes To
T3045 2550-2558 VB denotes increase
T3046 2559-2571 NN denotes palatability
T3047 2572-2575 CD denotes 0.2
T3048 2575-2576 NN denotes %
T3049 2577-2584 NN denotes sucrose
T3050 2585-2588 VBD denotes was
T3051 2589-2594 VBN denotes added
T3052 2595-2597 TO denotes to
T3053 2598-2601 DT denotes the
T3054 2602-2610 NN denotes drinking
T3055 2611-2616 NN denotes water
T3056 2617-2619 IN denotes of
T3057 2620-2623 DT denotes all
T3058 2624-2630 NNS denotes groups
T3059 2631-2632 -LRB- denotes (
T3060 2632-2635 NNP denotes Van
T3061 2636-2639 NN denotes der
T3062 2640-2645 NNP denotes Waaji
T3063 2646-2648 FW denotes et
T3064 2649-2652 FW denotes al.
T3065 2652-2653 , denotes ,
T3066 2654-2655 NNP denotes [
T3067 2655-2657 CD denotes 23
T3068 2657-2658 NNP denotes ]
T3069 2658-2659 -RRB- denotes )
T3070 2659-2660 . denotes .
T3071 2661-2666 JJ denotes Fresh
T3072 2667-2678 NN denotes carrageenan
T3073 2679-2688 NNS denotes solutions
T3074 2689-2693 VBD denotes were
T3075 2694-2702 JJ denotes prepared
T3076 2703-2708 JJ denotes daily
T3077 2708-2709 . denotes .
T3368 2711-2721 NN denotes Evaluation
T3369 2722-2724 IN denotes of
T3370 2725-2732 NNP denotes Colitis
T3371 2733-2737 NNP denotes Body
T3372 2738-2744 NN denotes weight
T3373 2744-2745 , denotes ,
T3374 2746-2752 NN denotes liquid
T3375 2753-2756 CC denotes and
T3376 2757-2761 NN denotes food
T3377 2762-2773 NN denotes consumption
T3378 2773-2774 , denotes ,
T3379 2775-2783 NN denotes diarrhea
T3380 2784-2787 CC denotes and
T3381 2788-2794 JJ denotes rectal
T3382 2795-2803 NN denotes bleeding
T3383 2804-2805 -LRB- denotes (
T3384 2805-2813 VBN denotes detected
T3385 2814-2816 IN denotes by
T3386 2817-2820 NN denotes eye
T3387 2821-2831 NN denotes inspection
T3388 2831-2832 -RRB- denotes )
T3389 2833-2837 VBD denotes were
T3390 2838-2846 VBN denotes recorded
T3391 2847-2857 IN denotes throughout
T3392 2858-2861 DT denotes the
T3393 2862-2869 NN denotes feeding
T3394 2870-2876 NN denotes period
T3395 2876-2877 . denotes .
T3396 2878-2883 IN denotes After
T3397 2884-2886 CD denotes 55
T3398 2887-2891 NNS denotes days
T3399 2891-2892 , denotes ,
T3400 2893-2900 NNS denotes animals
T3401 2901-2905 VBD denotes were
T3402 2906-2916 VBN denotes sacrificed
T3403 2917-2919 IN denotes by
T3404 2920-2928 JJ denotes cervical
T3405 2929-2940 NN denotes dislocation
T3406 2940-2941 . denotes .
T3407 2942-2945 DT denotes The
T3408 2946-2952 NN denotes length
T3409 2953-2955 IN denotes of
T3410 2956-2959 DT denotes the
T3411 2960-2965 NN denotes colon
T3412 2966-2969 VBD denotes was
T3413 2970-2978 VBN denotes measured
T3414 2979-2981 IN denotes as
T3415 2982-2991 VBN denotes described
T3416 2992-2994 IN denotes by
T3417 2995-3003 NNP denotes Okayashu
T3418 3004-3006 FW denotes et
T3419 3007-3010 FW denotes al.
T3420 3011-3012 NNP denotes [
T3421 3012-3014 CD denotes 24
T3422 3014-3015 NNP denotes ]
T3423 3015-3016 . denotes .
T3424 3017-3021 RB denotes Then
T3425 3021-3022 , denotes ,
T3426 3023-3027 DT denotes each
T3427 3028-3033 NN denotes colon
T3428 3034-3037 VBD denotes was
T3429 3038-3045 VBN denotes ligated
T3430 3046-3048 IN denotes in
T3431 3049-3057 NNS denotes sections
T3432 3058-3060 IN denotes of
T3433 3061-3062 CD denotes 2
T3434 3063-3065 NN denotes cm
T3435 3066-3069 CC denotes and
T3436 3070-3071 CD denotes 1
T3437 3072-3074 TO denotes to
T3438 3075-3076 CD denotes 2
T3439 3077-3079 NN denotes ml
T3440 3080-3082 IN denotes of
T3441 3083-3085 CD denotes 10
T3442 3085-3086 NN denotes %
T3443 3087-3095 NN denotes formalin
T3444 3096-3099 VBD denotes was
T3445 3100-3107 VBN denotes infused
T3446 3108-3112 IN denotes into
T3447 3113-3116 DT denotes the
T3448 3117-3127 JJ denotes intestinal
T3449 3128-3133 NNS denotes lumen
T3450 3133-3134 . denotes .
T3451 3135-3138 DT denotes The
T3452 3139-3149 RB denotes moderately
T3453 3150-3159 JJ denotes distended
T3454 3160-3167 NN denotes segment
T3455 3168-3171 VBD denotes was
T3456 3172-3181 VBN denotes sectioned
T3457 3182-3185 CC denotes and
T3458 3186-3191 VBN denotes fixed
T3459 3192-3194 IN denotes in
T3460 3195-3197 CD denotes 10
T3461 3197-3198 NN denotes %
T3462 3199-3207 NN denotes formalin
T3463 3207-3208 . denotes .
T3464 3209-3212 DT denotes The
T3465 3213-3222 JJ denotes following
T3466 3223-3226 NN denotes day
T3467 3226-3227 , denotes ,
T3468 3228-3231 DT denotes the
T3469 3232-3242 JJ denotes intestinal
T3470 3243-3250 NN denotes content
T3471 3251-3254 VBD denotes was
T3472 3255-3262 VBN denotes removed
T3473 3263-3265 IN denotes by
T3474 3266-3275 VBG denotes vortexing
T3475 3275-3276 . denotes .
T3476 3277-3280 DT denotes The
T3477 3281-3286 VBN denotes fixed
T3478 3287-3294 NN denotes segment
T3479 3295-3298 VBD denotes was
T3480 3299-3303 VBN denotes kept
T3481 3304-3306 IN denotes in
T3482 3307-3309 CD denotes 10
T3483 3309-3310 NN denotes %
T3484 3311-3319 NN denotes formalin
T3485 3320-3322 IN denotes at
T3486 3323-3324 CD denotes 4
T3487 3324-3325 CD denotes °
T3488 3325-3326 NNP denotes C
T3489 3327-3332 IN denotes until
T3490 3333-3336 DT denotes the
T3491 3337-3345 NN denotes paraffin
T3492 3346-3355 NN denotes embedding
T3493 3356-3365 NN denotes procedure
T3494 3365-3366 . denotes .
T3495 3367-3369 TO denotes To
T3496 3370-3378 VB denotes evaluate
T3497 3379-3382 DT denotes the
T3498 3383-3389 NN denotes degree
T3499 3390-3392 IN denotes of
T3500 3393-3405 NN denotes inflammation
T3501 3405-3406 , denotes ,
T3502 3407-3411 DT denotes this
T3503 3412-3419 NN denotes segment
T3504 3420-3422 IN denotes of
T3505 3423-3428 NN denotes colon
T3506 3429-3432 VBD denotes was
T3507 3433-3439 VBN denotes opened
T3508 3440-3454 RB denotes longitudinally
T3509 3455-3458 CC denotes and
T3510 3459-3470 JJ denotes macroscopic
T3511 3471-3474 CC denotes and
T3512 3475-3487 JJ denotes histological
T3513 3488-3494 NNS denotes scores
T3514 3495-3497 IN denotes of
T3515 3498-3510 NN denotes inflammation
T3516 3511-3515 VBD denotes were
T3517 3516-3524 VBN denotes recorded
T3518 3525-3527 IN denotes as
T3519 3528-3538 RB denotes previously
T3520 3539-3548 VBN denotes described
T3521 3549-3550 NNP denotes [
T3522 3550-3552 CD denotes 25
T3523 3552-3553 NNP denotes ]
T3524 3553-3554 , denotes ,
T3525 3555-3556 NNP denotes [
T3526 3556-3558 CD denotes 26
T3527 3558-3559 NNP denotes ]
T3528 3559-3560 . denotes .
T3529 3561-3564 DT denotes The
T3530 3565-3574 NN denotes toluidine
T3531 3575-3579 JJ denotes blue
T3532 3580-3588 NN denotes staining
T3533 3589-3592 VBD denotes was
T3534 3593-3597 VBN denotes used
T3535 3598-3601 IN denotes for
T3536 3602-3616 NN denotes identification
T3537 3617-3619 IN denotes of
T3538 3620-3629 VBN denotes sulphated
T3539 3630-3645 NNS denotes polysaccharides
T3540 3646-3648 IN denotes in
T3541 3649-3652 DT denotes the
T3542 3653-3663 JJ denotes intestinal
T3543 3664-3670 NN denotes mucosa
T3544 3670-3671 . denotes .
T3545 3672-3674 IN denotes On
T3546 3675-3678 DT denotes the
T3547 3679-3682 NN denotes day
T3548 3683-3685 IN denotes of
T3549 3686-3695 NN denotes sacrifice
T3550 3695-3696 , denotes ,
T3551 3697-3698 DT denotes a
T3552 3699-3704 JJ denotes fresh
T3553 3705-3711 NN denotes sample
T3554 3712-3714 IN denotes of
T3555 3715-3719 DT denotes each
T3556 3720-3725 NN denotes colon
T3557 3726-3727 -LRB- denotes (
T3558 3727-3729 CD denotes 50
T3559 3730-3732 NN denotes mg
T3560 3732-3733 -RRB- denotes )
T3561 3734-3737 VBD denotes was
T3562 3738-3747 VBN denotes collected
T3563 3748-3751 IN denotes for
T3564 3752-3767 NN denotes myeloperoxidase
T3565 3768-3769 -LRB- denotes (
T3566 3769-3772 NNP denotes MPO
T3567 3772-3773 -RRB- denotes )
T3568 3774-3779 JJ denotes assay
T3569 3780-3789 VBG denotes according
T3570 3790-3792 TO denotes to
T3571 3793-3800 NNP denotes Krawisz
T3572 3801-3803 FW denotes et
T3573 3804-3807 FW denotes al.
T3574 3807-3808 , denotes ,
T3575 3809-3810 NNP denotes [
T3576 3810-3812 CD denotes 27
T3577 3812-3813 NNP denotes ]
T3578 3813-3814 . denotes .
T3579 3815-3818 DT denotes The
T3580 3819-3824 NN denotes level
T3581 3825-3827 IN denotes of
T3582 3828-3831 NNP denotes MPO
T3583 3831-3832 , denotes ,
T3584 3833-3839 RB denotes mainly
T3585 3840-3849 VBN denotes expressed
T3586 3850-3852 IN denotes by
T3587 3853-3864 NNS denotes neutrophils
T3588 3864-3865 , denotes ,
T3589 3866-3875 VBZ denotes indicates
T3590 3876-3879 DT denotes the
T3591 3880-3884 NN denotes rate
T3592 3885-3887 IN denotes of
T3593 3888-3899 NN denotes recruitment
T3594 3900-3902 IN denotes of
T3595 3903-3914 NNS denotes neutrophils
T3596 3915-3917 TO denotes to
T3597 3918-3921 DT denotes the
T3598 3922-3932 JJ denotes intestinal
T3599 3933-3939 NN denotes mucosa
T3600 3939-3940 . denotes .
T3601 3941-3944 CD denotes One
T3602 3945-3949 NN denotes unit
T3603 3950-3952 IN denotes of
T3604 3953-3956 NNP denotes MPO
T3605 3957-3965 NN denotes activity
T3606 3966-3977 VBZ denotes corresponds
T3607 3978-3980 TO denotes to
T3608 3981-3984 DT denotes the
T3609 3985-3996 NN denotes degradation
T3610 3997-3999 IN denotes of
T3611 4000-4001 CD denotes 1
T3612 4002-4006 NN denotes µmol
T3613 4007-4009 IN denotes of
T3614 4010-4018 NN denotes peroxide
T3615 4019-4022 IN denotes per
T3616 4023-4029 NN denotes minute
T3617 4030-4032 IN denotes at
T3618 4033-4035 CD denotes 25
T3619 4035-4036 CD denotes °
T3620 4036-4038 NNP denotes C.
T3816 4040-4044 NNP denotes Cell
T3817 4045-4052 NNP denotes Culture
T3818 4053-4056 NNP denotes All
T3819 4057-4063 NN denotes tissue
T3820 4064-4071 NN denotes culture
T3821 4072-4080 NNS denotes reagents
T3822 4081-4085 VBD denotes were
T3823 4086-4090 IN denotes from
T3824 4091-4101 NNP denotes Invitrogen
T3825 4102-4103 -LRB- denotes (
T3826 4103-4108 NNP denotes Cergy
T3827 4109-4117 NNP denotes Pontoise
T3828 4117-4118 , denotes ,
T3829 4119-4125 NNP denotes France
T3830 4125-4126 -RRB- denotes )
T3831 4126-4127 . denotes .
T3832 4128-4133 CD denotes THP-1
T3833 4134-4139 JJ denotes human
T3834 4140-4149 JJ denotes monocytic
T3835 4150-4155 NNS denotes cells
T3836 4156-4160 VBD denotes were
T3837 4161-4171 VBN denotes maintained
T3838 4172-4174 IN denotes in
T3839 4175-4184 NN denotes RPMI-1640
T3840 4185-4197 VBN denotes supplemented
T3841 4198-4202 IN denotes with
T3842 4203-4205 CD denotes 10
T3843 4205-4206 NN denotes %
T3844 4207-4210 NNP denotes FCS
T3845 4210-4211 , denotes ,
T3846 4212-4213 CD denotes 2
T3847 4214-4216 NNP denotes mM
T3848 4217-4218 NNP denotes L
T3849 4219-4220 : denotes -
T3850 4220-4229 NN denotes glutamine
T3851 4229-4230 , denotes ,
T3852 4231-4233 CD denotes 50
T3853 4234-4238 NN denotes U/ml
T3854 4239-4249 NN denotes penicillin
T3855 4250-4253 CC denotes and
T3856 4254-4256 CD denotes 50
T3857 4257-4262 JJ denotes mg/ml
T3858 4263-4275 NN denotes streptomycin
T3859 4276-4278 IN denotes at
T3860 4279-4281 CD denotes 37
T3861 4281-4282 CD denotes °
T3862 4282-4283 NNP denotes C
T3863 4284-4286 IN denotes in
T3864 4287-4288 DT denotes a
T3865 4289-4290 CD denotes 5
T3866 4290-4291 NN denotes %
T3867 4292-4295 CD denotes CO2
T3868 4296-4305 NN denotes incubator
T3869 4305-4306 . denotes .
T3870 4307-4312 JJ denotes Human
T3871 4313-4323 JJ denotes peripheral
T3872 4324-4329 NN denotes blood
T3873 4330-4341 NN denotes mononuclear
T3874 4342-4347 NNS denotes cells
T3875 4348-4352 VBD denotes were
T3876 4353-4361 VBN denotes obtained
T3877 4362-4366 IN denotes from
T3878 4367-4378 VBN denotes heparinized
T3879 4379-4384 NN denotes blood
T3880 4385-4387 IN denotes by
T3881 4388-4402 JJ denotes Ficoll-Hypaque
T3882 4403-4410 NN denotes density
T3883 4411-4419 NN denotes gradient
T3884 4419-4420 . denotes .
T3885 4421-4430 NNS denotes Monocytes
T3886 4431-4435 VBD denotes were
T3887 4436-4440 RB denotes then
T3888 4441-4449 VBN denotes isolated
T3889 4450-4452 IN denotes by
T3890 4453-4462 NN denotes adherence
T3891 4463-4465 TO denotes to
T3892 4466-4473 NN denotes culture
T3893 4474-4480 NNS denotes flasks
T3894 4481-4483 IN denotes as
T3895 4484-4493 VBN denotes described
T3896 4494-4495 NNP denotes [
T3897 4495-4497 CD denotes 28
T3898 4497-4498 NNP denotes ]
T3899 4498-4499 . denotes .
T3900 4500-4503 IN denotes For
T3901 4504-4508 NN denotes cell
T3902 4509-4520 NN denotes aggregation
T3903 4520-4521 , denotes ,
T3904 4522-4531 NNS denotes monocytes
T3905 4532-4536 VBD denotes were
T3906 4537-4545 VBN denotes cultured
T3907 4546-4548 IN denotes in
T3908 4549-4552 DT denotes the
T3909 4553-4561 NN denotes presence
T3910 4562-4564 CC denotes or
T3911 4565-4572 NN denotes absence
T3912 4573-4575 IN denotes of
T3913 4576-4579 CD denotes C10
T3914 4580-4582 CC denotes or
T3915 4583-4586 CD denotes C40
T3916 4587-4590 IN denotes for
T3917 4591-4593 CD denotes 72
T3918 4594-4596 NN denotes h.
T3919 4597-4601 NNP denotes Cell
T3920 4602-4610 NNS denotes colonies
T3921 4611-4615 VBD denotes were
T3922 4616-4625 VBN denotes monitored
T3923 4626-4631 IN denotes under
T3924 4632-4634 DT denotes an
T3925 4635-4643 JJ denotes inverted
T3926 4644-4649 NN denotes phase
T3927 4650-4658 NN denotes contrast
T3928 4659-4669 NN denotes microscope
T3929 4670-4677 VBN denotes coupled
T3930 4678-4685 IN denotes through
T3931 4686-4687 DT denotes a
T3932 4688-4693 NN denotes video
T3933 4694-4700 NN denotes camera
T3934 4701-4703 TO denotes to
T3935 4704-4705 DT denotes a
T3936 4706-4714 NN denotes computer
T3937 4714-4715 . denotes .
T3938 4716-4718 IN denotes In
T3939 4719-4723 DT denotes some
T3940 4724-4729 NNS denotes wells
T3941 4729-4730 , denotes ,
T3942 4731-4743 VBG denotes neutralizing
T3943 4744-4754 JJ denotes monoclonal
T3944 4755-4763 NN denotes antibody
T3945 4764-4766 TO denotes to
T3946 4767-4773 NNP denotes ICAM-1
T3947 4774-4775 -LRB- denotes (
T3948 4775-4778 CD denotes 2.5
T3949 4779-4781 NN denotes µg
T3950 4781-4782 NN denotes /
T3951 4782-4784 NN denotes ml
T3952 4784-4785 -RRB- denotes )
T3953 4786-4787 -LRB- denotes (
T3954 4787-4791 NNP denotes Tebu
T3955 4791-4792 , denotes ,
T3956 4793-4795 NNP denotes Le
T3957 4796-4802 NNP denotes Perray
T3958 4803-4805 IN denotes en
T3959 4806-4814 NNP denotes Yvelines
T3960 4814-4815 , denotes ,
T3961 4816-4822 NNP denotes France
T3962 4822-4823 -RRB- denotes )
T3963 4824-4827 VBD denotes was
T3964 4828-4833 VBN denotes added
T3965 4833-4834 . denotes .
T4100 4836-4840 NNP denotes Cell
T4101 4841-4846 NNP denotes Cycle
T4102 4847-4855 NNP denotes Analysis
T4103 4856-4861 NNP denotes THP-1
T4104 4862-4867 NNS denotes cells
T4105 4868-4870 IN denotes in
T4106 4871-4882 JJ denotes exponential
T4107 4883-4889 NN denotes growth
T4108 4890-4895 NN denotes phase
T4109 4896-4900 VBD denotes were
T4110 4901-4908 VBN denotes exposed
T4111 4909-4911 TO denotes to
T4112 4912-4920 VB denotes complete
T4113 4921-4927 NN denotes medium
T4114 4928-4930 IN denotes in
T4115 4931-4934 DT denotes the
T4116 4935-4943 NN denotes presence
T4117 4944-4946 CC denotes or
T4118 4947-4954 NN denotes absence
T4119 4955-4957 IN denotes of
T4120 4958-4970 NNS denotes carrageenans
T4121 4971-4974 IN denotes for
T4122 4975-4977 CD denotes 24
T4123 4978-4979 NN denotes h
T4124 4980-4986 IN denotes before
T4125 4987-4992 VBG denotes being
T4126 4993-5000 VBN denotes stained
T4127 5001-5005 IN denotes with
T4128 5006-5015 NN denotes propidium
T4129 5016-5022 NN denotes iodide
T4130 5023-5028 VBG denotes using
T4131 5029-5032 DT denotes the
T4132 5033-5041 NNP denotes DNA-Prep
T4133 5042-5049 NNP denotes Coulter
T4134 5050-5053 NN denotes kit
T4135 5054-5063 VBG denotes according
T4136 5064-5066 TO denotes to
T4137 5067-5070 DT denotes the
T4138 5071-5083 NN denotes manufacturer
T4139 5083-5085 POS denotes 's
T4140 5086-5097 NN denotes instruction
T4141 5098-5099 -LRB- denotes (
T4142 5099-5114 NNP denotes Beckman-Coulter
T4143 5114-5115 , denotes ,
T4144 5116-5126 NNP denotes Villepinte
T4145 5126-5127 , denotes ,
T4146 5128-5134 NNP denotes France
T4147 5134-5135 -RRB- denotes )
T4148 5135-5136 . denotes .
T4149 5137-5141 NNP denotes Cell
T4150 5142-5145 NNP denotes DNA
T4151 5146-5153 NN denotes content
T4152 5154-5157 VBD denotes was
T4153 5158-5162 RB denotes then
T4154 5163-5171 VBN denotes analyzed
T4155 5172-5174 IN denotes by
T4156 5175-5179 NN denotes flow
T4157 5180-5189 NN denotes cytometry
T4158 5190-5195 VBG denotes using
T4159 5196-5198 DT denotes an
T4160 5199-5204 NNP denotes EPICS
T4161 5205-5208 NNP denotes XL2
T4162 5209-5210 -LRB- denotes (
T4163 5210-5225 NNP denotes Beckman-Coulter
T4164 5225-5226 -RRB- denotes )
T4165 5226-5227 . denotes .
T4166 5228-5231 JJ denotes Raw
T4167 5232-5236 NNS denotes data
T4168 5237-5240 IN denotes for
T4169 5241-5244 DT denotes the
T4170 5245-5257 NN denotes distribution
T4171 5258-5260 IN denotes of
T4172 5261-5264 NNP denotes DNA
T4173 5265-5272 NN denotes content
T4174 5273-5275 IN denotes of
T4175 5276-5282 CD denotes 30,000
T4176 5283-5288 NNS denotes cells
T4177 5289-5298 VBN denotes retrieved
T4178 5299-5303 IN denotes from
T4179 5304-5307 DT denotes the
T4180 5308-5317 NN denotes cytometer
T4181 5318-5322 VBD denotes were
T4182 5323-5332 VBN denotes expressed
T4183 5333-5335 IN denotes as
T4184 5336-5339 DT denotes the
T4185 5340-5350 NN denotes percentage
T4186 5351-5353 IN denotes of
T4187 5354-5359 CD denotes G0/G1
T4188 5360-5367 IN denotes through
T4189 5368-5372 NNP denotes G2/M
T4190 5373-5384 NNS denotes populations
T4191 5384-5385 . denotes .
T4192 5386-5396 NNP denotes Multicycle
T4193 5397-5399 NNP denotes AV
T4194 5400-5408 NN denotes software
T4195 5409-5410 -LRB- denotes (
T4196 5410-5417 NNP denotes Phoenix
T4197 5418-5422 NNP denotes Flow
T4198 5423-5430 NNPS denotes Systems
T4199 5430-5431 , denotes ,
T4200 5432-5435 NNP denotes San
T4201 5436-5441 NNP denotes Diego
T4202 5441-5442 , denotes ,
T4203 5443-5445 NNP denotes CA
T4204 5445-5446 -RRB- denotes )
T4205 5447-5450 VBD denotes was
T4206 5451-5455 VBN denotes used
T4207 5456-5458 TO denotes to
T4208 5459-5467 VB denotes generate
T4209 5468-5471 NNP denotes DNA
T4210 5472-5479 JJ denotes content
T4211 5480-5489 NN denotes frequency
T4212 5490-5500 NNS denotes histograms
T4213 5501-5504 CC denotes and
T4214 5505-5515 VB denotes facilitate
T4215 5516-5520 NNS denotes data
T4216 5521-5529 NN denotes analysis
T4217 5529-5530 . denotes .
T4382 5532-5536 NNP denotes Cell
T4383 5537-5544 NNP denotes Surface
T4384 5545-5552 NNP denotes Antigen
T4385 5553-5563 NNP denotes Expression
T4386 5564-5572 NNP denotes Analysis
T4387 5573-5583 NNP denotes Peripheral
T4388 5584-5589 NNP denotes Blood
T4389 5590-5599 NNPS denotes Monocytes
T4390 5600-5602 CC denotes or
T4391 5603-5608 CD denotes THP-1
T4392 5609-5614 NNS denotes cells
T4393 5615-5619 VBD denotes were
T4394 5620-5627 VBN denotes exposed
T4395 5628-5630 TO denotes to
T4396 5631-5639 VB denotes complete
T4397 5640-5646 NN denotes medium
T4398 5647-5649 IN denotes in
T4399 5650-5653 DT denotes the
T4400 5654-5662 NN denotes presence
T4401 5663-5665 CC denotes or
T4402 5666-5673 NN denotes absence
T4403 5674-5676 IN denotes of
T4404 5677-5688 NN denotes carrageenan
T4405 5689-5692 IN denotes for
T4406 5693-5695 CD denotes 36
T4407 5696-5697 NN denotes h
T4408 5697-5698 . denotes .
T4409 5699-5704 IN denotes After
T4410 5705-5708 CD denotes two
T4411 5709-5715 NNS denotes washes
T4412 5716-5718 IN denotes in
T4413 5719-5722 NNP denotes PBS
T4414 5723-5730 IN denotes without
T4415 5731-5734 NNP denotes Ca2
T4416 5734-5735 NNP denotes +
T4417 5736-5739 CC denotes and
T4418 5740-5743 NNP denotes Mg2
T4419 5743-5744 NNP denotes +
T4420 5744-5745 , denotes ,
T4421 5746-5751 NNS denotes cells
T4422 5752-5756 VBD denotes were
T4423 5757-5766 VBN denotes incubated
T4424 5767-5769 IN denotes in
T4425 5770-5773 NNP denotes PBS
T4426 5774-5784 VBG denotes containing
T4427 5785-5788 CD denotes 0.1
T4428 5788-5789 NN denotes %
T4429 5790-5797 NN denotes gelatin
T4430 5798-5801 CC denotes and
T4431 5802-5803 CD denotes 8
T4432 5803-5804 NN denotes %
T4433 5805-5807 NNP denotes AB
T4434 5808-5813 JJ denotes human
T4435 5814-5819 NN denotes serum
T4436 5820-5822 TO denotes to
T4437 5823-5830 VB denotes prevent
T4438 5831-5838 JJ denotes binding
T4439 5839-5841 TO denotes to
T4440 5842-5844 VB denotes Fc
T4441 5845-5854 NNS denotes receptors
T4442 5854-5855 . denotes .
T4443 5856-5860 RB denotes Then
T4444 5860-5861 , denotes ,
T4445 5862-5863 CD denotes 5
T4446 5863-5864 NN denotes ×
T4447 5864-5867 CD denotes 105
T4448 5868-5873 NNS denotes cells
T4449 5874-5878 VBD denotes were
T4450 5879-5888 VBN denotes incubated
T4451 5889-5893 IN denotes with
T4452 5894-5901 JJ denotes primary
T4453 5902-5912 NNS denotes antibodies
T4454 5913-5915 IN denotes at
T4455 5916-5917 CD denotes 4
T4456 5917-5918 CD denotes °
T4457 5918-5919 NNP denotes C
T4458 5920-5923 IN denotes for
T4459 5924-5926 CD denotes 30
T4460 5927-5930 NN denotes min
T4461 5930-5931 . denotes .
T4462 5932-5935 CD denotes Two
T4463 5936-5941 JJ denotes other
T4464 5942-5948 NNS denotes washes
T4465 5949-5951 IN denotes in
T4466 5952-5955 NNP denotes PBS
T4467 5956-5964 VBD denotes preceded
T4468 5965-5975 NN denotes incubation
T4469 5976-5980 IN denotes with
T4470 5981-5996 JJ denotes FITC-conjugated
T4471 5997-6001 NN denotes goat
T4472 6002-6010 NN denotes antibody
T4473 6011-6021 JJ denotes anti-mouse
T4474 6022-6025 NNP denotes IgG
T4475 6026-6033 VBD denotes diluted
T4476 6034-6040 CD denotes 1/1000
T4477 6041-6043 IN denotes at
T4478 6044-6045 CD denotes 4
T4479 6045-6046 CD denotes °
T4480 6046-6047 NNP denotes C
T4481 6048-6051 IN denotes for
T4482 6052-6054 CD denotes 30
T4483 6055-6058 NN denotes min
T4484 6059-6060 -LRB- denotes (
T4485 6060-6064 NNP denotes Tebu
T4486 6064-6065 -RRB- denotes )
T4487 6065-6066 . denotes .
T4488 6067-6072 IN denotes After
T4489 6073-6076 CD denotes two
T4490 6077-6087 JJ denotes additional
T4491 6088-6094 NNS denotes washes
T4492 6094-6095 , denotes ,
T4493 6096-6104 NN denotes analysis
T4494 6105-6107 IN denotes of
T4495 6108-6115 JJ denotes stained
T4496 6116-6121 NNS denotes cells
T4497 6122-6125 VBD denotes was
T4498 6126-6135 VBN denotes performed
T4499 6136-6138 IN denotes on
T4500 6139-6141 DT denotes an
T4501 6142-6147 NNP denotes EPICS
T4502 6148-6151 NNP denotes XL2
T4503 6152-6153 -LRB- denotes (
T4504 6153-6168 NNP denotes Beckman-Coulter
T4505 6168-6169 -RRB- denotes )
T4506 6169-6170 . denotes .
T4507 6171-6174 DT denotes The
T4508 6175-6179 NN denotes cell
T4509 6180-6190 NN denotes population
T4510 6191-6194 VBD denotes was
T4511 6195-6200 VBN denotes gated
T4512 6201-6210 VBG denotes according
T4513 6211-6213 TO denotes to
T4514 6214-6217 PRP$ denotes its
T4515 6218-6225 JJ denotes forward
T4516 6226-6229 CC denotes and
T4517 6230-6240 JJ denotes wide-angle
T4518 6241-6246 JJ denotes light
T4519 6247-6257 NN denotes scattering
T4520 6257-6258 . denotes .
T4521 6259-6263 NNS denotes Data
T4522 6264-6268 VBD denotes were
T4523 6269-6278 VBN denotes expressed
T4524 6279-6281 IN denotes as
T4525 6282-6286 JJ denotes mean
T4526 6287-6295 JJ denotes relative
T4527 6296-6308 NN denotes fluorescence
T4528 6309-6318 NN denotes intensity
T4529 6319-6320 -LRB- denotes (
T4530 6320-6323 NNP denotes MFI
T4531 6323-6324 -RRB- denotes )
T4532 6325-6327 IN denotes of
T4533 6328-6332 CD denotes 3000
T4534 6333-6338 NNS denotes cells
T4535 6338-6339 . denotes .
T4648 6341-6344 NNP denotes TNF
T4649 6345-6353 NNP denotes Activity
T4650 6354-6362 NNP denotes Bioassay
T4651 6363-6372 NNPS denotes Monocytes
T4652 6373-6375 CC denotes or
T4653 6376-6381 CD denotes THP-1
T4654 6382-6387 NNS denotes cells
T4655 6388-6392 VBD denotes were
T4656 6393-6401 VBN denotes cultured
T4657 6402-6406 IN denotes with
T4658 6407-6409 CC denotes or
T4659 6410-6417 IN denotes without
T4660 6418-6427 JJ denotes different
T4661 6428-6442 NNS denotes concentrations
T4662 6443-6445 IN denotes of
T4663 6446-6450 NNS denotes CGNs
T4664 6451-6453 CC denotes or
T4665 6454-6457 NNP denotes LPS
T4666 6458-6459 -LRB- denotes (
T4667 6459-6469 NNP denotes Salmonella
T4668 6470-6477 NN denotes typhosa
T4669 6477-6478 , denotes ,
T4670 6479-6484 NNP denotes Sigma
T4671 6484-6485 -RRB- denotes )
T4672 6486-6489 IN denotes for
T4673 6490-6492 CD denotes 24
T4674 6493-6494 NN denotes h
T4675 6495-6497 CC denotes or
T4676 6498-6501 DT denotes the
T4677 6502-6511 JJ denotes indicated
T4678 6512-6516 NN denotes time
T4679 6516-6517 . denotes .
T4680 6518-6530 RB denotes Biologically
T4681 6531-6537 JJ denotes active
T4682 6538-6543 NNP denotes TNF-α
T4683 6543-6544 NN denotes /
T4684 6544-6545 NN denotes β
T4685 6546-6548 IN denotes in
T4686 6549-6555 NN denotes tissue
T4687 6556-6563 NN denotes culture
T4688 6564-6575 NN denotes supernatant
T4689 6576-6579 VBD denotes was
T4690 6580-6588 VBN denotes measured
T4691 6589-6594 VBG denotes using
T4692 6595-6598 DT denotes the
T4693 6599-6603 NNP denotes WEHI
T4694 6604-6607 CD denotes 164
T4695 6608-6613 NN denotes clone
T4696 6614-6621 JJ denotes 13-cell
T4697 6622-6629 VBG denotes killing
T4698 6630-6635 NN denotes assay
T4699 6636-6637 NNP denotes [
T4700 6637-6639 CD denotes 29
T4701 6639-6640 NNP denotes ]
T4702 6640-6641 . denotes .
T4703 6642-6645 NNP denotes TNF
T4704 6646-6660 NNS denotes concentrations
T4705 6661-6664 VBP denotes are
T4706 6665-6674 VBN denotes expressed
T4707 6675-6677 IN denotes as
T4708 6678-6683 NN denotes pg/ml
T4709 6683-6684 . denotes .
T4951 6686-6692 NNP denotes RT-PCR
T4952 6693-6701 NNP denotes Analysis
T4953 6702-6707 NNP denotes Total
T4954 6708-6711 NNP denotes RNA
T4955 6712-6716 IN denotes from
T4956 6717-6726 NNS denotes monocytes
T4957 6727-6730 VBD denotes was
T4958 6731-6739 VBN denotes isolated
T4959 6740-6745 VBG denotes using
T4960 6746-6752 NNP denotes TRIzol
T4961 6753-6760 NNP denotes Reagent
T4962 6760-6761 NNP denotes ™
T4963 6762-6763 -LRB- denotes (
T4964 6763-6773 NNP denotes Invitrogen
T4965 6773-6774 -RRB- denotes )
T4966 6774-6775 . denotes .
T4967 6776-6780 NNP denotes cDNA
T4968 6781-6784 VBD denotes was
T4969 6785-6794 VBN denotes generated
T4970 6795-6797 IN denotes on
T4971 6798-6799 CD denotes 1
T4972 6800-6802 NN denotes µg
T4973 6803-6805 IN denotes of
T4974 6806-6811 JJ denotes total
T4975 6812-6815 NNP denotes RNA
T4976 6816-6818 IN denotes in
T4977 6819-6820 DT denotes a
T4978 6821-6829 NN denotes reaction
T4979 6830-6836 NN denotes volume
T4980 6837-6839 IN denotes of
T4981 6840-6842 CD denotes 20
T4982 6843-6845 NN denotes µl
T4983 6845-6846 , denotes ,
T4984 6847-6852 VBG denotes using
T4985 6853-6858 NNP denotes M-MLV
T4986 6859-6866 VB denotes reverse
T4987 6867-6880 NN denotes transcriptase
T4988 6881-6882 -LRB- denotes (
T4989 6882-6892 NNP denotes Invitrogen
T4990 6892-6893 -RRB- denotes )
T4991 6893-6894 . denotes .
T4992 6895-6898 NNP denotes PCR
T4993 6899-6902 VBD denotes was
T4994 6903-6907 VBN denotes done
T4995 6908-6910 IN denotes in
T4996 6911-6914 DT denotes the
T4997 6915-6921 JJ denotes linear
T4998 6922-6927 NN denotes range
T4999 6928-6930 IN denotes of
T5000 6931-6944 NN denotes amplification
T5001 6945-6946 -LRB- denotes (
T5002 6946-6956 VBN denotes determined
T5003 6957-6960 IN denotes for
T5004 6961-6965 DT denotes each
T5005 6966-6972 NN denotes primer
T5006 6973-6982 NN denotes pair-cDNA
T5007 6983-6994 NN denotes combination
T5008 6994-6995 -RRB- denotes )
T5009 6995-6996 . denotes .
T5010 6997-7005 NNP denotes Standard
T5011 7006-7009 NNP denotes PCR
T5012 7010-7019 NNS denotes reactions
T5013 7020-7024 VBD denotes were
T5014 7025-7034 VBN denotes performed
T5015 7035-7039 IN denotes with
T5016 7040-7041 CD denotes 1
T5017 7042-7044 NN denotes µl
T5018 7045-7047 IN denotes of
T5019 7048-7051 DT denotes the
T5020 7052-7056 NNP denotes cDNA
T5021 7057-7065 NN denotes solution
T5022 7065-7066 , denotes ,
T5023 7067-7069 CD denotes 50
T5024 7070-7072 NN denotes µM
T5025 7073-7075 IN denotes of
T5026 7076-7080 DT denotes each
T5027 7081-7087 NN denotes primer
T5028 7088-7096 NN denotes solution
T5029 7096-7097 , denotes ,
T5030 7098-7100 CD denotes 10
T5031 7101-7103 NN denotes mM
T5032 7104-7106 IN denotes of
T5033 7107-7111 DT denotes each
T5034 7112-7116 NNP denotes dNTP
T5035 7116-7117 , denotes ,
T5036 7118-7120 CD denotes 25
T5037 7121-7123 NNP denotes mM
T5038 7124-7129 NNP denotes MgCl2
T5039 7129-7130 , denotes ,
T5040 7131-7134 NNP denotes 10X
T5041 7135-7143 NNP denotes Goldstar
T5042 7144-7147 NNP denotes DNA
T5043 7148-7158 NN denotes polymerase
T5044 7159-7167 NN denotes reaction
T5045 7168-7174 NN denotes buffer
T5046 7174-7175 , denotes ,
T5047 7176-7179 CC denotes and
T5048 7180-7183 CD denotes 0.5
T5049 7184-7189 NNS denotes units
T5050 7190-7192 IN denotes of
T5051 7193-7201 NNP denotes Goldstar
T5052 7202-7205 NNP denotes DNA
T5053 7206-7216 NN denotes polymerase
T5054 7217-7218 -LRB- denotes (
T5055 7218-7228 NNP denotes Eurogentec
T5056 7228-7229 , denotes ,
T5057 7230-7237 NNP denotes Seraing
T5058 7237-7238 , denotes ,
T5059 7239-7246 NNP denotes Belgium
T5060 7246-7247 -RRB- denotes )
T5061 7247-7248 . denotes .
T5062 7249-7254 NNP denotes First
T5063 7255-7258 NNP denotes PCR
T5064 7259-7264 NN denotes cycle
T5065 7265-7274 VBD denotes consisted
T5066 7275-7277 IN denotes of
T5067 7278-7279 CD denotes 1
T5068 7280-7283 NN denotes min
T5069 7284-7286 IN denotes at
T5070 7287-7289 CD denotes 92
T5071 7289-7290 CD denotes °
T5072 7290-7291 NNP denotes C
T5073 7291-7292 , denotes ,
T5074 7293-7294 CD denotes 1
T5075 7295-7298 NN denotes min
T5076 7299-7301 IN denotes at
T5077 7302-7304 CD denotes 58
T5078 7304-7305 CD denotes °
T5079 7305-7306 NNP denotes C
T5080 7307-7310 CC denotes and
T5081 7311-7312 CD denotes 1
T5082 7313-7316 NN denotes min
T5083 7317-7319 IN denotes at
T5084 7320-7322 CD denotes 72
T5085 7322-7323 CD denotes °
T5086 7323-7324 NNP denotes C
T5087 7324-7325 : denotes ;
T5088 7326-7330 RB denotes then
T5089 7331-7335 DT denotes each
T5090 7336-7339 NNP denotes PCR
T5091 7340-7345 NN denotes cycle
T5092 7346-7355 VBD denotes consisted
T5093 7356-7358 IN denotes of
T5094 7359-7361 CD denotes 40
T5095 7362-7365 NN denotes sec
T5096 7366-7368 IN denotes at
T5097 7369-7371 CD denotes 92
T5098 7371-7372 CD denotes °
T5099 7372-7373 NNP denotes C
T5100 7373-7374 , denotes ,
T5101 7375-7377 CD denotes 40
T5102 7378-7381 NN denotes sec
T5103 7382-7384 IN denotes at
T5104 7385-7387 CD denotes 58
T5105 7387-7388 CD denotes °
T5106 7388-7389 NNP denotes C
T5107 7390-7393 CC denotes and
T5108 7394-7396 CD denotes 50
T5109 7397-7400 NN denotes sec
T5110 7401-7403 IN denotes at
T5111 7404-7406 CD denotes 72
T5112 7406-7407 CD denotes °
T5113 7407-7409 NNP denotes C.
T5114 7410-7414 NNP denotes cDNA
T5115 7415-7418 IN denotes for
T5116 7419-7426 NN denotes β-actin
T5117 7427-7430 VBD denotes was
T5118 7431-7440 VBN denotes amplified
T5119 7441-7444 IN denotes for
T5120 7445-7447 CD denotes 28
T5121 7448-7454 NNS denotes cycles
T5122 7455-7460 VBG denotes using
T5123 7461-7464 DT denotes the
T5124 7465-7471 NNS denotes oligos
T5125 7471-7472 : denotes :
T5126 7473-7478 NN denotes sense
T5127 7479-7480 CD denotes 5
T5128 7480-7481 SYM denotes ′
T5129 7481-7482 : denotes -
T5130 7482-7503 NN denotes GGCATCGTGATGGACTCCG-3
T5131 7503-7504 NN denotes ′
T5132 7505-7508 CC denotes and
T5133 7509-7518 JJ denotes antisense
T5134 7519-7520 CD denotes 5
T5135 7520-7521 CD denotes ′
T5136 7521-7542 NNP denotes GCTGGAAGGTGGACAGCGA-3
T5137 7542-7543 NN denotes ′
T5138 7543-7544 . denotes .
T5139 7545-7549 NNP denotes cDNA
T5140 7550-7553 IN denotes for
T5141 7554-7559 NNP denotes TNF-α
T5142 7560-7563 VBD denotes was
T5143 7564-7573 VBN denotes amplified
T5144 7574-7577 IN denotes for
T5145 7578-7580 CD denotes 35
T5146 7581-7587 NNS denotes cycles
T5147 7588-7593 VBG denotes using
T5148 7594-7597 DT denotes the
T5149 7598-7604 NNS denotes oligos
T5150 7604-7605 : denotes :
T5151 7606-7611 NN denotes sense
T5152 7612-7613 CD denotes 5
T5153 7613-7614 SYM denotes ′
T5154 7614-7615 : denotes -
T5155 7615-7637 NN denotes AAGCCTGTAGCCCATGTTGT-3
T5156 7637-7638 NN denotes ′
T5157 7639-7642 CC denotes and
T5158 7643-7652 JJ denotes antisense
T5159 7653-7654 CD denotes 5
T5160 7654-7655 SYM denotes ′
T5161 7655-7656 : denotes -
T5162 7656-7678 NN denotes CAGATAGATGGGCTCATACC-3
T5163 7678-7679 NN denotes ′
T5164 7679-7680 . denotes .
T5165 7681-7685 NNP denotes cDNA
T5166 7686-7689 IN denotes for
T5167 7690-7696 NNP denotes ICAM-1
T5168 7697-7700 VBD denotes was
T5169 7701-7710 VBN denotes amplified
T5170 7711-7714 IN denotes for
T5171 7715-7717 CD denotes 35
T5172 7718-7724 NNS denotes cycles
T5173 7725-7730 VBG denotes using
T5174 7731-7734 DT denotes the
T5175 7735-7741 NNS denotes oligos
T5176 7742-7747 VBP denotes sense
T5177 7748-7749 CD denotes 5
T5178 7749-7750 SYM denotes ′
T5179 7750-7751 : denotes -
T5180 7751-7775 NN denotes GTAGCAGCCGCAGTCATAATGG-3
T5181 7775-7776 NN denotes ′
T5182 7777-7780 CC denotes and
T5183 7781-7790 JJ denotes antisense
T5184 7791-7792 CD denotes 5
T5185 7792-7793 SYM denotes ′
T5186 7793-7794 : denotes -
T5187 7794-7795 DT denotes A
T5188 7796-7819 JJ denotes TGCTGTTGTATCTGACTGAGG-3
T5189 7819-7820 NN denotes ′
T5190 7820-7821 . denotes .
T5335 7823-7828 NNP denotes NF-kB
T5336 7829-7842 NNP denotes Transcription
T5337 7843-7851 NNP denotes Reporter
T5338 7852-7856 NNP denotes Gene
T5339 7857-7862 NNP denotes Assay
T5340 7863-7866 NNP denotes The
T5341 7867-7874 VBD denotes plasmid
T5342 7875-7884 NNP denotes 3XMHC-luc
T5343 7885-7886 -LRB- denotes (
T5344 7886-7887 DT denotes a
T5345 7888-7896 JJ denotes generous
T5346 7897-7901 NN denotes gift
T5347 7902-7906 IN denotes from
T5348 7907-7911 NNP denotes Drs.
T5349 7912-7914 NNP denotes J.
T5350 7915-7923 NNP denotes Westwick
T5351 7924-7927 CC denotes and
T5352 7928-7932 NNP denotes D.A.
T5353 7933-7940 NNP denotes Brenner
T5354 7940-7941 , denotes ,
T5355 7942-7952 NNP denotes University
T5356 7953-7955 IN denotes of
T5357 7956-7961 NNP denotes North
T5358 7962-7970 NNP denotes Carolina
T5359 7970-7971 , denotes ,
T5360 7972-7978 NNP denotes Chapel
T5361 7979-7983 NNP denotes Hill
T5362 7983-7984 -RRB- denotes )
T5363 7985-7993 VBZ denotes contains
T5364 7994-7999 CD denotes three
T5365 8000-8006 NNS denotes copies
T5366 8007-8009 IN denotes of
T5367 8010-8026 JJ denotes NF-κB-responsive
T5368 8027-8034 NN denotes element
T5369 8035-8039 IN denotes from
T5370 8040-8043 DT denotes the
T5371 8044-8047 NNP denotes MHC
T5372 8048-8053 NN denotes class
T5373 8054-8055 PRP denotes I
T5374 8056-8061 VBZ denotes locus
T5375 8061-8062 , denotes ,
T5376 8063-8069 VBN denotes placed
T5377 8070-8078 RB denotes upstream
T5378 8079-8081 IN denotes of
T5379 8082-8085 DT denotes the
T5380 8086-8096 NN denotes luciferase
T5381 8097-8101 NN denotes gene
T5382 8101-8102 . denotes .
T5383 8103-8108 JJ denotes Human
T5384 8109-8118 JJ denotes monocytic
T5385 8119-8124 NN denotes THP-1
T5386 8125-8130 NNS denotes cells
T5387 8131-8135 VBD denotes were
T5388 8136-8147 RB denotes transiently
T5389 8148-8159 VBN denotes transfected
T5390 8160-8162 IN denotes as
T5391 8163-8173 RB denotes previously
T5392 8174-8183 VBN denotes described
T5393 8184-8185 NNP denotes [
T5394 8185-8187 CD denotes 30
T5395 8187-8188 NNP denotes ]
T5396 8188-8189 , denotes ,
T5397 8190-8193 CC denotes and
T5398 8194-8198 RB denotes then
T5399 8199-8207 VBD denotes cultured
T5400 8208-8211 IN denotes for
T5401 8212-8213 CD denotes 4
T5402 8214-8215 NN denotes h
T5403 8216-8221 RB denotes alone
T5404 8222-8224 CC denotes or
T5405 8225-8229 IN denotes with
T5406 8230-8240 VBG denotes increasing
T5407 8241-8254 NN denotes concentration
T5408 8255-8257 IN denotes of
T5409 8258-8264 DT denotes either
T5410 8265-8268 NNP denotes C10
T5411 8269-8271 CC denotes or
T5412 8272-8275 NNP denotes C40
T5413 8275-8276 . denotes .
T5414 8277-8287 JJ denotes Luciferase
T5415 8288-8296 NN denotes activity
T5416 8297-8300 VBD denotes was
T5417 8301-8311 VBN denotes determined
T5418 8312-8317 VBG denotes using
T5419 8318-8319 DT denotes a
T5420 8320-8331 NN denotes luminometer
T5421 8332-8333 -LRB- denotes (
T5422 8333-8342 NNP denotes Monolight
T5423 8343-8347 CD denotes 2010
T5424 8348-8359 NNP denotes Luminometer
T5425 8359-8360 , denotes ,
T5426 8361-8364 NNP denotes Ann
T5427 8365-8370 NNP denotes Arbor
T5428 8370-8371 , denotes ,
T5429 8372-8374 NNP denotes MI
T5430 8374-8375 -RRB- denotes )
T5431 8375-8376 . denotes .
T5933 8378-8385 JJ denotes Western
T5934 8386-8390 NNP denotes Blot
T5935 8391-8399 NNP denotes Analysis
T5936 8400-8405 NNP denotes THP-1
T5937 8406-8411 NNS denotes cells
T5938 8412-8416 VBD denotes were
T5939 8417-8427 VBN denotes stimulated
T5940 8428-8431 IN denotes for
T5941 8432-8439 JJ denotes various
T5942 8440-8447 NNS denotes lengths
T5943 8448-8450 IN denotes of
T5944 8451-8455 NN denotes time
T5945 8456-8460 IN denotes with
T5946 8461-8464 CD denotes 0.1
T5947 8465-8470 NN denotes mg/ml
T5948 8471-8474 CD denotes C10
T5949 8475-8477 CC denotes or
T5950 8478-8481 CD denotes C40
T5951 8481-8482 , denotes ,
T5952 8483-8485 CC denotes or
T5953 8486-8488 CD denotes 10
T5954 8489-8491 NN denotes µg
T5955 8491-8492 NN denotes /
T5956 8492-8494 NN denotes ml
T5957 8495-8498 NNP denotes LPS
T5958 8498-8499 . denotes .
T5959 8500-8505 NNS denotes Cells
T5960 8506-8510 VBD denotes were
T5961 8511-8515 RB denotes then
T5962 8516-8524 VBN denotes pelleted
T5963 8524-8525 , denotes ,
T5964 8526-8532 VBN denotes washed
T5965 8533-8536 CC denotes and
T5966 8537-8548 VBN denotes homogenised
T5967 8549-8551 IN denotes in
T5968 8552-8557 NN denotes lysis
T5969 8558-8564 NN denotes buffer
T5970 8565-8566 -LRB- denotes (
T5971 8566-8568 CD denotes 10
T5972 8569-8571 NNP denotes mM
T5973 8572-8577 NNP denotes Hepes
T5974 8577-8578 , denotes ,
T5975 8579-8581 NNP denotes pH
T5976 8582-8585 CD denotes 7.9
T5977 8585-8586 , denotes ,
T5978 8587-8590 CD denotes 150
T5979 8591-8593 NNP denotes mM
T5980 8594-8598 NNP denotes NaCl
T5981 8598-8599 , denotes ,
T5982 8600-8601 CD denotes 1
T5983 8602-8604 NNP denotes mM
T5984 8605-8609 NNP denotes EDTA
T5985 8609-8610 , denotes ,
T5986 8611-8614 CD denotes 0.6
T5987 8614-8615 NN denotes %
T5988 8616-8621 NN denotes NP-40
T5989 8621-8622 , denotes ,
T5990 8623-8626 CC denotes and
T5991 8627-8630 CD denotes 0.5
T5992 8631-8633 NNP denotes mM
T5993 8634-8638 NNP denotes PMSF
T5994 8638-8639 -RRB- denotes )
T5995 8640-8642 IN denotes on
T5996 8643-8646 NN denotes ice
T5997 8646-8647 . denotes .
T5998 8648-8659 NNS denotes Homogenates
T5999 8660-8664 VBD denotes were
T6000 8665-8674 VBN denotes sonicated
T6001 8674-8675 , denotes ,
T6002 8676-8687 VBN denotes centrifuged
T6003 8688-8690 IN denotes at
T6004 8691-8697 CD denotes 10,000
T6005 8698-8701 NN denotes rpm
T6006 8702-8704 TO denotes to
T6007 8705-8711 VB denotes remove
T6008 8712-8720 JJ denotes cellular
T6009 8721-8727 NN denotes debris
T6010 8727-8728 , denotes ,
T6011 8729-8732 CC denotes and
T6012 8733-8744 JJ denotes supernatant
T6013 8745-8754 VBN denotes collected
T6014 8754-8755 . denotes .
T6015 8756-8763 NN denotes Protein
T6016 8764-8777 NN denotes concentration
T6017 8778-8781 VBD denotes was
T6018 8782-8792 VBN denotes determined
T6019 8793-8798 VBG denotes using
T6020 8799-8802 DT denotes the
T6021 8803-8805 NNP denotes DC
T6022 8806-8813 NNP denotes Protein
T6023 8814-8819 NNP denotes Assay
T6024 8820-8821 -LRB- denotes (
T6025 8821-8828 NNP denotes Bio-Rad
T6026 8828-8829 -RRB- denotes )
T6027 8829-8830 . denotes .
T6028 8831-8839 NNPS denotes Proteins
T6029 8840-8842 IN denotes in
T6030 8843-8850 NNS denotes samples
T6031 8851-8852 -LRB- denotes (
T6032 8852-8854 CD denotes 15
T6033 8855-8857 NN denotes µg
T6034 8858-8863 JJ denotes total
T6035 8864-8872 NNS denotes proteins
T6036 8872-8873 -RRB- denotes )
T6037 8874-8878 VBD denotes were
T6038 8879-8887 VBN denotes resolved
T6039 8888-8890 IN denotes in
T6040 8891-8892 DT denotes a
T6041 8893-8903 VBG denotes denaturing
T6042 8904-8906 CD denotes 12
T6043 8906-8907 NN denotes %
T6044 8908-8922 NN denotes polyacrylamide
T6045 8923-8926 NN denotes gel
T6046 8927-8930 CC denotes and
T6047 8931-8942 VBN denotes transferred
T6048 8943-8945 TO denotes to
T6049 8946-8947 DT denotes a
T6050 8948-8962 JJ denotes nitrocellulose
T6051 8963-8971 NN denotes membrane
T6052 8971-8972 . denotes .
T6053 8973-8978 JJ denotes I-κBα
T6054 8979-8986 NN denotes protein
T6055 8987-8990 VBD denotes was
T6056 8991-8999 VBN denotes detected
T6057 9000-9005 VBG denotes using
T6058 9006-9007 DT denotes a
T6059 9008-9014 NN denotes rabbit
T6060 9015-9025 NN denotes polyclonal
T6061 9026-9034 NN denotes antibody
T6062 9035-9036 -LRB- denotes (
T6063 9036-9041 NNP denotes Santa
T6064 9042-9046 NNP denotes Cruz
T6065 9047-9060 NNP denotes Biotechnology
T6066 9060-9061 , denotes ,
T6067 9062-9064 NNP denotes CA
T6068 9064-9065 -RRB- denotes )
T6069 9066-9074 VBN denotes followed
T6070 9075-9077 IN denotes by
T6071 9078-9079 DT denotes a
T6072 9080-9091 JJ denotes horseradish
T6073 9092-9110 JJ denotes peroxidase-coupled
T6074 9111-9115 NN denotes goat
T6075 9116-9126 NN denotes polyclonal
T6076 9127-9135 NN denotes antibody
T6077 9136-9143 IN denotes against
T6078 9144-9150 NN denotes rabbit
T6079 9151-9153 NNP denotes Ig
T6080 9154-9155 -LRB- denotes (
T6081 9155-9161 NNP denotes Caltag
T6082 9162-9174 NNPS denotes Laboratories
T6083 9174-9175 -RRB- denotes )
T6084 9175-9176 . denotes .
T6085 9177-9184 RB denotes Finally
T6086 9184-9185 , denotes ,
T6087 9186-9189 NNP denotes IκB
T6088 9190-9195 NNS denotes bands
T6089 9196-9200 VBD denotes were
T6090 9201-9209 VBN denotes revealed
T6091 9210-9215 VBG denotes using
T6092 9216-9219 DT denotes the
T6093 9220-9223 NNP denotes ECL
T6094 9223-9224 NN denotes ™
T6095 9225-9234 NN denotes detection
T6096 9235-9241 NN denotes system
T6097 9242-9243 -LRB- denotes (
T6098 9243-9251 NNP denotes Amersham
T6099 9252-9261 NNP denotes Pharmacia
T6100 9262-9269 NNP denotes Biotech
T6101 9269-9270 , denotes ,
T6102 9271-9274 NNP denotes Les
T6103 9275-9280 NNP denotes Ullis
T6104 9280-9281 , denotes ,
T6105 9282-9288 NNP denotes France
T6106 9288-9289 -RRB- denotes )
T6107 9290-9299 VBG denotes according
T6108 9300-9302 TO denotes to
T6109 9303-9306 DT denotes the
T6110 9307-9320 NNS denotes manufacturers
T6111 9320-9321 POS denotes '
T6112 9322-9333 NN denotes instruction
T6113 9333-9334 . denotes .
T6114 9335-9343 NN denotes Antibody
T6115 9344-9346 TO denotes to
T6116 9347-9356 NNP denotes α-Tubulin
T6117 9357-9358 -LRB- denotes (
T6118 9358-9363 NNP denotes Santa
T6119 9364-9368 NNP denotes Cruz
T6120 9368-9369 -RRB- denotes )
T6121 9370-9373 VBD denotes was
T6122 9374-9377 NN denotes use
T6123 9378-9380 IN denotes as
T6124 9381-9388 VBG denotes loading
T6125 9389-9396 NN denotes control
T6126 9396-9397 . denotes .
T6127 9398-9401 IN denotes For
T6128 9402-9409 JJ denotes nuclear
T6129 9410-9415 NNP denotes NF-κB
T6130 9415-9416 , denotes ,
T6131 9417-9422 NNP denotes THP-1
T6132 9423-9428 NNS denotes cells
T6133 9429-9433 VBD denotes were
T6134 9434-9444 VBN denotes stimulated
T6135 9445-9449 IN denotes with
T6136 9450-9451 CD denotes 1
T6137 9452-9457 NN denotes mg/ml
T6138 9458-9461 CD denotes C10
T6139 9462-9464 CC denotes or
T6140 9465-9468 CD denotes C40
T6141 9469-9472 IN denotes for
T6142 9473-9475 CD denotes 30
T6143 9476-9483 NNS denotes minutes
T6144 9484-9486 IN denotes at
T6145 9487-9489 CD denotes 37
T6146 9489-9490 NN denotes °
T6147 9490-9492 NNP denotes C.
T6148 9493-9498 NNP denotes Cells
T6149 9499-9503 VBD denotes were
T6150 9504-9508 RB denotes then
T6151 9509-9517 VBN denotes pelleted
T6152 9518-9521 CC denotes and
T6153 9522-9528 JJ denotes nuclei
T6154 9529-9538 VBN denotes separated
T6155 9539-9541 IN denotes as
T6156 9542-9551 VBN denotes described
T6157 9552-9553 NNP denotes [
T6158 9553-9555 CD denotes 31
T6159 9555-9556 NNP denotes ]
T6160 9556-9557 . denotes .
T6161 9558-9564 NNP denotes Nuclei
T6162 9565-9569 VBD denotes were
T6163 9570-9576 VBN denotes washed
T6164 9577-9580 CC denotes and
T6165 9581-9592 VBN denotes homogenized
T6166 9593-9601 RB denotes directly
T6167 9602-9604 IN denotes in
T6168 9605-9612 VBG denotes loading
T6169 9613-9614 -LRB- denotes (
T6170 9614-9620 NNP denotes Laemli
T6171 9620-9621 -RRB- denotes )
T6172 9622-9628 NN denotes buffer
T6173 9629-9632 CC denotes and
T6174 9633-9639 VBN denotes heated
T6175 9640-9643 IN denotes for
T6176 9644-9645 CD denotes 5
T6177 9646-9653 NNS denotes minutes
T6178 9654-9656 IN denotes at
T6179 9657-9660 CD denotes 100
T6180 9660-9661 CD denotes °
T6181 9661-9663 NNP denotes C.
T6182 9664-9672 NNPS denotes Proteins
T6183 9673-9675 IN denotes in
T6184 9676-9683 NNS denotes samples
T6185 9684-9688 VBD denotes were
T6186 9689-9697 VBN denotes resolved
T6187 9698-9700 IN denotes in
T6188 9701-9702 DT denotes a
T6189 9703-9713 NN denotes denaturing
T6190 9714-9715 CD denotes 8
T6191 9715-9716 NN denotes %
T6192 9717-9731 NN denotes polyacrylamide
T6193 9732-9735 NN denotes gel
T6194 9736-9739 CC denotes and
T6195 9740-9751 VBN denotes transferred
T6196 9752-9754 TO denotes to
T6197 9755-9756 DT denotes a
T6198 9757-9771 NN denotes polyvinylidine
T6199 9772-9780 NN denotes fluoride
T6200 9781-9782 -LRB- denotes (
T6201 9782-9786 NNP denotes PVDF
T6202 9786-9787 -RRB- denotes )
T6203 9788-9796 NN denotes membrane
T6204 9797-9798 -LRB- denotes (
T6205 9798-9809 JJ denotes Immobilon-P
T6206 9809-9810 : denotes ;
T6207 9811-9820 NNP denotes Millipore
T6208 9820-9821 , denotes ,
T6209 9822-9829 NNP denotes Bedford
T6210 9829-9830 , denotes ,
T6211 9831-9833 NNP denotes MA
T6212 9833-9834 -RRB- denotes )
T6213 9834-9835 . denotes .
T6214 9836-9845 NNS denotes Membranes
T6215 9846-9850 VBD denotes were
T6216 9851-9860 VBN denotes incubated
T6217 9861-9863 IN denotes in
T6218 9864-9872 VBG denotes blocking
T6219 9873-9879 NN denotes buffer
T6220 9880-9881 -LRB- denotes (
T6221 9881-9882 CD denotes 1
T6222 9882-9883 NN denotes %
T6223 9884-9887 NNP denotes BSA
T6224 9887-9888 , denotes ,
T6225 9889-9891 IN denotes in
T6226 9892-9895 NNP denotes PBS
T6227 9895-9896 -RRB- denotes )
T6228 9897-9900 IN denotes for
T6229 9901-9904 CD denotes two
T6230 9905-9910 NNS denotes hours
T6231 9911-9913 IN denotes at
T6232 9914-9918 NN denotes room
T6233 9919-9930 NN denotes temperature
T6234 9930-9931 . denotes .
T6235 9932-9941 NNS denotes Membranes
T6236 9942-9946 VBD denotes were
T6237 9947-9959 RB denotes subsequently
T6238 9960-9966 VBN denotes probed
T6239 9967-9971 IN denotes with
T6240 9972-9975 DT denotes the
T6241 9976-9989 JJ denotes corresponding
T6242 9990-9998 NN denotes antibody
T6243 9999-10001 IN denotes in
T6244 10002-10010 VBG denotes blocking
T6245 10011-10017 NN denotes buffer
T6246 10017-10018 , denotes ,
T6247 10019-10028 JJ denotes overnight
T6248 10028-10029 . denotes .
T6249 10030-10036 NN denotes Rabbit
T6250 10037-10047 NN denotes polyclonal
T6251 10048-10056 NN denotes antibody
T6252 10057-10067 JJ denotes anti-NF-κB
T6253 10068-10071 JJ denotes p50
T6254 10072-10079 NN denotes subunit
T6255 10080-10081 -LRB- denotes (
T6256 10081-10082 # denotes #
T6257 10083-10089 CD denotes sc-114
T6258 10089-10090 -RRB- denotes )
T6259 10091-10093 CC denotes or
T6260 10094-10104 JJ denotes anti-NF-κB
T6261 10105-10108 NNS denotes p65
T6262 10109-10116 VBN denotes subunit
T6263 10117-10118 -LRB- denotes (
T6264 10118-10119 # denotes #
T6265 10120-10126 CD denotes sc-109
T6266 10126-10127 -RRB- denotes )
T6267 10128-10132 IN denotes from
T6268 10133-10138 NNP denotes Santa
T6269 10139-10143 NNP denotes Cruz
T6270 10144-10157 NNP denotes Biotechnology
T6271 10158-10162 VBD denotes were
T6272 10163-10167 VBN denotes used
T6273 10167-10168 . denotes .
T6274 10169-10178 NNS denotes Membranes
T6275 10179-10183 VBD denotes were
T6276 10184-10190 VBN denotes washed
T6277 10191-10194 CD denotes six
T6278 10195-10200 NNS denotes times
T6279 10201-10203 IN denotes in
T6280 10204-10207 NNP denotes PBS
T6281 10208-10212 IN denotes with
T6282 10213-10217 CD denotes 0.05
T6283 10217-10218 NN denotes %
T6284 10219-10224 CD denotes Tween
T6285 10225-10227 CD denotes 20
T6286 10227-10228 , denotes ,
T6287 10229-10230 CD denotes 5
T6288 10231-10238 NNS denotes minutes
T6289 10239-10243 DT denotes each
T6290 10244-10248 NN denotes time
T6291 10248-10249 , denotes ,
T6292 10250-10253 CC denotes and
T6293 10254-10263 VBN denotes incubated
T6294 10264-10268 IN denotes with
T6295 10269-10270 DT denotes a
T6296 10271-10277 CD denotes 1/3000
T6297 10278-10286 NN denotes dilution
T6298 10287-10289 IN denotes of
T6299 10290-10304 JJ denotes HRP-conjugated
T6300 10305-10306 NN denotes F
T6301 10306-10307 -LRB- denotes (
T6302 10307-10309 NN denotes ab
T6303 10309-10310 '' denotes '
T6304 10310-10311 -RRB- denotes )
T6305 10311-10312 CD denotes 2
T6306 10313-10317 NN denotes goat
T6307 10318-10329 JJ denotes anti-rabbit
T6308 10330-10333 NNP denotes IgG
T6309 10334-10336 IN denotes in
T6310 10337-10338 CD denotes 5
T6311 10338-10339 NN denotes %
T6312 10340-10346 JJ denotes nonfat
T6313 10347-10350 JJ denotes dry
T6314 10351-10355 NN denotes milk
T6315 10356-10359 CC denotes and
T6316 10360-10364 CD denotes 0.05
T6317 10364-10365 NN denotes %
T6318 10366-10371 CD denotes Tween
T6319 10372-10374 CD denotes 20
T6320 10375-10377 IN denotes in
T6321 10378-10381 NNP denotes PBS
T6322 10382-10385 IN denotes for
T6323 10386-10387 CD denotes 1
T6324 10388-10392 NN denotes hour
T6325 10393-10395 IN denotes at
T6326 10396-10400 NN denotes room
T6327 10401-10412 NN denotes temperature
T6328 10412-10413 . denotes .
T6329 10414-10419 IN denotes After
T6330 10420-10427 VBG denotes washing
T6331 10428-10431 CD denotes six
T6332 10432-10436 JJR denotes more
T6333 10437-10442 NNS denotes times
T6334 10443-10445 IN denotes in
T6335 10446-10449 NNP denotes PBS
T6336 10450-10454 IN denotes with
T6337 10455-10459 CD denotes 0.05
T6338 10459-10460 NN denotes %
T6339 10461-10466 CD denotes Tween
T6340 10467-10469 CD denotes 20
T6341 10469-10470 , denotes ,
T6342 10471-10488 JJ denotes antibody-reactive
T6343 10489-10497 NNS denotes proteins
T6344 10498-10502 VBD denotes were
T6345 10503-10511 VBN denotes detected
T6346 10512-10517 VBG denotes using
T6347 10518-10519 DT denotes a
T6348 10520-10537 NN denotes chemiluminescence
T6349 10538-10547 NN denotes substrate
T6350 10548-10549 -LRB- denotes (
T6351 10549-10560 NNP denotes SuperSignal
T6352 10560-10561 : denotes ;
T6353 10562-10568 NNP denotes Pierce
T6354 10568-10569 , denotes ,
T6355 10570-10578 NNP denotes Rockford
T6356 10578-10579 , denotes ,
T6357 10580-10582 NNP denotes IL
T6358 10582-10583 -RRB- denotes )
T6359 10584-10593 VBG denotes according
T6360 10594-10596 TO denotes to
T6361 10597-10600 DT denotes the
T6362 10601-10613 NN denotes manufacturer
T6363 10613-10615 POS denotes 's
T6364 10616-10628 NNS denotes instructions
T6365 10628-10629 . denotes .
T6366 10630-10632 TO denotes To
T6367 10633-10640 VB denotes confirm
T6368 10641-10645 DT denotes that
T6369 10646-10656 JJ denotes equivalent
T6370 10657-10664 NNS denotes amounts
T6371 10665-10667 IN denotes of
T6372 10668-10675 NN denotes protein
T6373 10676-10680 VBD denotes were
T6374 10681-10687 VBN denotes loaded
T6375 10688-10690 IN denotes in
T6376 10691-10695 DT denotes each
T6377 10696-10700 NN denotes line
T6378 10700-10701 , denotes ,
T6379 10702-10711 NNS denotes membranes
T6380 10712-10716 VBD denotes were
T6381 10717-10721 RB denotes also
T6382 10722-10729 JJ denotes Western
T6383 10730-10737 VBN denotes blotted
T6384 10738-10741 IN denotes for
T6385 10742-10745 NNP denotes ERK
T6386 10746-10748 IN denotes as
T6387 10749-10758 VBN denotes described
T6388 10759-10760 NNP denotes [
T6389 10760-10762 CD denotes 32
T6390 10762-10763 NNP denotes ]
T6391 10763-10764 . denotes .
T6486 10766-10774 NN denotes Analysis
T6487 10775-10777 IN denotes of
T6488 10778-10783 NNP denotes NF-κB
T6489 10784-10794 NNP denotes Activation
T6490 10795-10797 IN denotes by
T6491 10798-10802 NNP denotes Flow
T6492 10803-10812 NNP denotes Cytometry
T6493 10813-10820 NNP denotes Nuclear
T6494 10821-10831 NN denotes activation
T6495 10832-10834 IN denotes of
T6496 10835-10837 NNP denotes NF
T6497 10837-10838 NNP denotes −
T6498 10838-10840 NNP denotes κΒ
T6499 10841-10843 IN denotes by
T6500 10844-10848 NN denotes flow
T6501 10849-10858 NN denotes cytometry
T6502 10859-10862 VBD denotes was
T6503 10863-10872 VBN denotes performed
T6504 10873-10875 IN denotes as
T6505 10876-10885 VBN denotes described
T6506 10886-10887 NNP denotes [
T6507 10887-10889 CD denotes 31
T6508 10889-10890 NNP denotes ]
T6509 10890-10891 . denotes .
T6558 10893-10904 NNP denotes Statistical
T6559 10905-10913 NNP denotes Analysis
T6560 10914-10917 NNP denotes The
T6561 10918-10925 NNS denotes results
T6562 10926-10930 VBD denotes were
T6563 10931-10940 VBN denotes expressed
T6564 10941-10943 IN denotes as
T6565 10944-10947 DT denotes the
T6566 10948-10952 JJ denotes mean
T6567 10953-10958 NN denotes value
T6568 10959-10960 NN denotes ±
T6569 10961-10967 NNP denotes S.E.M.
T6570 10968-10970 IN denotes of
T6571 10971-10981 JJ denotes individual
T6572 10982-10993 NNS denotes experiments
T6573 10993-10994 . denotes .
T6574 10995-10998 DT denotes The
T6575 10999-11010 JJ denotes statistical
T6576 11011-11023 NN denotes significance
T6577 11024-11026 IN denotes of
T6578 11027-11030 DT denotes the
T6579 11031-11042 NNS denotes differences
T6580 11043-11050 IN denotes between
T6581 11051-11055 JJ denotes mean
T6582 11056-11062 NNS denotes values
T6583 11063-11066 VBD denotes was
T6584 11067-11075 VBN denotes assessed
T6585 11076-11078 IN denotes by
T6586 11079-11082 DT denotes the
T6587 11083-11090 NN denotes Student
T6588 11090-11092 POS denotes 's
T6589 11093-11099 JJS denotes t-test
T6590 11100-11103 CC denotes and
T6591 11104-11112 NN denotes analysis
T6592 11113-11115 IN denotes of
T6593 11116-11124 NN denotes variance
T6594 11125-11126 -LRB- denotes (
T6595 11126-11131 NNP denotes ANOVA
T6596 11131-11132 -RRB- denotes )
T6597 11132-11133 . denotes .
R2132 T2416 T2449 nsubjpass Preparation,prepared
R2133 T2417 T2416 prep of,Preparation
R2134 T2418 T2419 compound Degraded,Carrageenan
R2135 T2419 T2417 pobj Carrageenan,of
R2136 T2420 T2421 nummod Two,preparations
R2137 T2421 T2447 nmod preparations,weight
R2138 T2422 T2421 prep of,preparations
R2139 T2423 T2424 amod degraded,carrageenan
R2140 T2424 T2422 pobj carrageenan,of
R2141 T2425 T2424 prep with,carrageenan
R2142 T2426 T2425 pobj low,with
R2143 T2427 T2421 punct ",",preparations
R2144 T2428 T2429 punct (,∼
R2145 T2429 T2421 appos ∼,preparations
R2146 T2430 T2431 nummod 10,kDa
R2147 T2431 T2429 npadvmod kDa,∼
R2148 T2432 T2421 punct ;,preparations
R2149 T2433 T2421 appos C10,preparations
R2150 T2434 T2421 punct ),preparations
R2151 T2435 T2421 punct ",",preparations
R2152 T2436 T2421 cc and,preparations
R2180 T2464 T2463 punct ",",Industry
R2181 T2465 T2463 conj Boulogne-Billancourt,Industry
R2182 T2466 T2465 punct ",",Boulogne-Billancourt
R2183 T2467 T2465 conj France,Boulogne-Billancourt
R2184 T2468 T2452 punct ),iota-carrageenan
R2185 T2469 T2449 punct .,prepared
R2186 T2470 T2471 amod Native,carrageenan
R2187 T2471 T2473 nsubjpass carrageenan,dissolved
R2188 T2472 T2473 auxpass was,dissolved
R2189 T2473 T2473 ROOT dissolved,dissolved
R2190 T2474 T2473 prep in,dissolved
R2191 T2475 T2476 amod distilled,water
R2192 T2476 T2474 pobj water,in
R2193 T2477 T2480 punct (,w/v
R2194 T2478 T2479 nummod 5,%
R2195 T2479 T2480 compound %,w/v
R2196 T2480 T2473 parataxis w/v,dissolved
R2197 T2481 T2480 punct ),w/v
R2198 T2482 T2473 prep under,dissolved
R2199 T2483 T2484 amod vigorous,stirring
R2200 T2484 T2482 pobj stirring,under
R2201 T2485 T2484 cc and,stirring
R2202 T2486 T2484 conj heated,stirring
R2203 T2487 T2486 prep to,heated
R2204 T2488 T2489 nummod 60,°
R2232 T2516 T2518 amod low,weight
R2233 T2517 T2518 amod molecular,weight
R2234 T2518 T2519 compound weight,fraction
R2235 T2519 T2514 pobj fraction,for
R2236 T2520 T2524 punct ",",hydrolyzed
R2237 T2521 T2522 compound carrageenan,solution
R2238 T2522 T2524 nsubjpass solution,hydrolyzed
R2239 T2523 T2524 auxpass was,hydrolyzed
R2240 T2524 T2524 ROOT hydrolyzed,hydrolyzed
R2241 T2525 T2524 prep with,hydrolyzed
R2242 T2526 T2527 nummod 0.3,%
R2243 T2527 T2525 pobj %,with
R2244 T2528 T2529 punct (,v/v
R2245 T2529 T2527 appos v/v,%
R2246 T2530 T2524 punct ),hydrolyzed
R2247 T2531 T2533 nmod concentrated,acid
R2248 T2532 T2533 compound sulphuric,acid
R2249 T2533 T2524 dobj acid,hydrolyzed
R2250 T2534 T2524 prep for,hydrolyzed
R2251 T2535 T2536 nummod 15,min
R2252 T2536 T2534 pobj min,for
R2253 T2537 T2524 prep at,hydrolyzed
R2254 T2538 T2540 nummod 80,C
R2255 T2539 T2540 nummod °,C
R2256 T2540 T2537 pobj C,at
R2257 T2541 T2524 punct .,hydrolyzed
R2258 T2542 T2552 prep After,filtered
R2259 T2543 T2542 pobj neutralization,After
R2260 T2544 T2543 prep with,neutralization
R2261 T2545 T2546 compound NaOH,4N
R2262 T2546 T2544 pobj 4N,with
R2263 T2547 T2552 punct ",",filtered
R2264 T2548 T2549 det the,solution
R2265 T2549 T2552 nsubjpass solution,filtered
R2266 T2550 T2552 auxpass was,filtered
R2267 T2551 T2552 advmod ultra,filtered
R2268 T2552 T2552 ROOT filtered,filtered
R2269 T2553 T2552 prep through,filtered
R2270 T2554 T2557 det a,cartridge
R2271 T2555 T2557 amod hollow,cartridge
R2272 T2556 T2557 compound fibre,cartridge
R2273 T2557 T2553 pobj cartridge,through
R2274 T2558 T2557 prep with,cartridge
R2275 T2559 T2562 nmod MW,kDa
R2276 T2560 T2562 nmod cut-off,kDa
R2277 T2561 T2562 nummod 5,kDa
R2278 T2562 T2558 pobj kDa,with
R2279 T2563 T2562 punct ",",kDa
R2280 T2564 T2566 punct (,Inc
R2281 T2565 T2566 compound Amicon,Inc
R2282 T2566 T2562 conj Inc,kDa
R2283 T2567 T2566 punct ",",Inc
R2284 T2568 T2566 conj Beverly,Inc
R2285 T2569 T2568 punct ",",Beverly
R2286 T2570 T2568 conj USA,Beverly
R2287 T2571 T2566 punct ),Inc
R2288 T2572 T2552 punct .,filtered
R2289 T2573 T2584 prep For,hydrolyzed
R2290 T2574 T2578 det the,fraction
R2291 T2575 T2578 compound medium,fraction
R2292 T2576 T2577 amod molecular,weight
R2293 T2577 T2578 compound weight,fraction
R2294 T2578 T2573 pobj fraction,For
R2295 T2579 T2584 punct ",",hydrolyzed
R2296 T2580 T2582 det the,solution
R2297 T2581 T2582 compound carrageenan,solution
R2298 T2582 T2584 nsubjpass solution,hydrolyzed
R2299 T2583 T2584 auxpass was,hydrolyzed
R2300 T2584 T2584 ROOT hydrolyzed,hydrolyzed
R2301 T2585 T2584 prep with,hydrolyzed
R2302 T2586 T2587 nummod 0.3,%
R2303 T2587 T2585 pobj %,with
R2304 T2588 T2589 punct (,v/v
R2305 T2589 T2587 appos v/v,%
R2306 T2590 T2584 punct ),hydrolyzed
R2307 T2591 T2591 ROOT concentrated,concentrated
R2308 T2592 T2593 amod sulphuric,acid
R2309 T2593 T2591 dobj acid,concentrated
R2310 T2594 T2591 prep for,concentrated
R2311 T2595 T2596 nummod 30,min
R2312 T2596 T2594 pobj min,for
R2313 T2597 T2591 prep at,concentrated
R2314 T2598 T2599 nummod 60,°
R2315 T2599 T2597 pobj °,at
R2316 T2600 T2591 npadvmod C,concentrated
R2317 T2601 T2584 punct .,hydrolyzed
R2318 T2602 T2609 prep After,filtered
R2319 T2603 T2602 pobj neutralization,After
R2320 T2604 T2609 punct ",",filtered
R2321 T2605 T2606 det the,supernatant
R2322 T2606 T2609 nsubjpass supernatant,filtered
R2323 T2607 T2609 auxpass was,filtered
R2324 T2608 T2609 advmod ultra,filtered
R2325 T2609 T2609 ROOT filtered,filtered
R2326 T2610 T2614 punct (,kDa
R2327 T2611 T2614 prt MW,kDa
R2328 T2612 T2614 nmod cut-off,kDa
R2329 T2613 T2614 nummod 100,kDa
R2330 T2614 T2609 dobj kDa,filtered
R2331 T2615 T2614 punct ),kDa
R2332 T2616 T2609 punct .,filtered
R2333 T2617 T2618 det The,filtrate
R2334 T2618 T2620 nsubjpass filtrate,submitted
R2335 T2619 T2620 auxpass was,submitted
R2336 T2620 T2620 ROOT submitted,submitted
R2337 T2621 T2620 prep to,submitted
R2338 T2622 T2625 det a,filtration
R2339 T2623 T2625 amod second,filtration
R2340 T2624 T2625 compound ultra,filtration
R2341 T2625 T2621 pobj filtration,to
R2342 T2626 T2630 punct (,kDa
R2343 T2627 T2630 nmod MW,kDa
R2344 T2628 T2630 punct cut-off,kDa
R2345 T2629 T2628 nummod 5,cut-off
R2346 T2630 T2625 appos kDa,filtration
R2347 T2631 T2630 punct ),kDa
R2348 T2632 T2620 punct .,submitted
R2349 T2633 T2634 det Both,preparations
R2350 T2634 T2638 nsubjpass preparations,precipitated
R2351 T2635 T2634 prep of,preparations
R2352 T2636 T2635 pobj dCGN,of
R2353 T2637 T2638 auxpass were,precipitated
R2354 T2638 T2638 ROOT precipitated,precipitated
R2355 T2639 T2638 prep with,precipitated
R2356 T2640 T2641 nummod 4,volumes
R2357 T2641 T2639 pobj volumes,with
R2358 T2642 T2641 prep of,volumes
R2359 T2643 T2644 nummod 95,%
R2360 T2644 T2645 compound %,ethanol
R2361 T2645 T2642 pobj ethanol,of
R2362 T2646 T2638 punct ",",precipitated
R2363 T2647 T2638 conj dried,precipitated
R2364 T2648 T2647 prep at,dried
R2365 T2649 T2650 compound room,temperature
R2366 T2650 T2648 pobj temperature,at
R2367 T2651 T2650 cc and,temperature
R2368 T2652 T2650 conj ground,temperature
R2369 T2653 T2647 prep to,dried
R2370 T2654 T2655 amod small,particles
R2371 T2655 T2653 pobj particles,to
R2372 T2656 T2658 punct (,mm
R2373 T2657 T2658 nummod 1,mm
R2374 T2658 T2655 parataxis mm,particles
R2375 T2659 T2658 prep in,mm
R2376 T2660 T2659 pobj diameter,in
R2377 T2661 T2658 punct ),mm
R2378 T2662 T2638 punct .,precipitated
R2379 T2663 T2663 ROOT Using,Using
R2380 T2664 T2665 compound gel-permeation,chromatography
R2381 T2665 T2663 dobj chromatography,Using
R2382 T2666 T2665 prep in,chromatography
R2383 T2667 T2666 pobj combination,in
R2384 T2668 T2663 prep with,Using
R2385 T2669 T2671 amod light,measurements
R2386 T2670 T2671 compound scattering,measurements
R2387 T2671 T2668 pobj measurements,with
R2388 T2672 T2673 punct (,see
R2389 T2673 T2663 parataxis see,Using
R2390 T2674 T2684 nsubj Viebke,confirmed
R2391 T2675 T2676 compound et,al.
R2392 T2676 T2684 prep al.,confirmed
R2393 T2677 T2676 appos [,al.
R2394 T2678 T2679 nummod 21,]
R2395 T2679 T2677 appos ],[
R2396 T2680 T2677 punct ),[
R2397 T2681 T2684 punct ",",confirmed
R2398 T2682 T2684 nsubjpass it,confirmed
R2399 T2683 T2684 auxpass was,confirmed
R2400 T2684 T2673 ccomp confirmed,see
R2401 T2685 T2689 mark that,had
R2402 T2686 T2688 det the,fraction
R2403 T2687 T2688 amod low,fraction
R2404 T2688 T2689 nsubj fraction,had
R2405 T2689 T2684 ccomp had,confirmed
R2406 T2690 T2693 det an,weight
R2407 T2691 T2693 amod average,weight
R2408 T2692 T2693 amod molecular,weight
R2409 T2693 T2689 dobj weight,had
R2410 T2694 T2693 prep of,weight
R2411 T2695 T2696 nummod 10,kDa
R2412 T2696 T2694 pobj kDa,of
R2413 T2697 T2689 punct ",",had
R2414 T2698 T2689 cc and,had
R2415 T2699 T2701 det the,fraction
R2416 T2700 T2701 compound medium,fraction
R2417 T2701 T2689 conj fraction,had
R2418 T2702 T2701 prep of,fraction
R2419 T2703 T2704 nummod 40,kDa
R2420 T2704 T2702 pobj kDa,of
R2421 T2705 T2673 punct .,see
R2422 T2706 T2708 det The,content
R2423 T2707 T2708 compound sulphate,content
R2424 T2708 T2715 nsubjpass content,measured
R2425 T2709 T2708 prep of,content
R2426 T2710 T2709 pobj polysaccharides,of
R2427 T2711 T2710 prep in,polysaccharides
R2428 T2712 T2713 det both,fractions
R2429 T2713 T2711 pobj fractions,in
R2430 T2714 T2715 auxpass was,measured
R2431 T2715 T2715 ROOT measured,measured
R2432 T2716 T2715 prep following,measured
R2433 T2717 T2718 det the,method
R2434 T2718 T2716 pobj method,following
R2435 T2719 T2718 prep of,method
R2436 T2720 T2723 compound Quemener,[
R2437 T2721 T2722 compound et,al.
R2438 T2722 T2723 compound al.,[
R2439 T2723 T2719 pobj [,of
R2440 T2724 T2725 nummod 22,]
R2441 T2725 T2718 npadvmod ],method
R2442 T2726 T2715 punct .,measured
R2443 T2727 T2740 advmod Finally,confirmed
R2444 T2728 T2740 punct ",",confirmed
R2445 T2729 T2730 det the,absence
R2446 T2730 T2740 nsubjpass absence,confirmed
R2447 T2731 T2730 prep of,absence
R2448 T2732 T2733 compound polysaccharide,structure
R2449 T2733 T2734 compound structure,modifications
R2450 T2734 T2731 pobj modifications,of
R2451 T2735 T2734 prep in,modifications
R2452 T2736 T2738 det the,fractions
R2453 T2737 T2738 nummod two,fractions
R2454 T2738 T2735 pobj fractions,in
R2455 T2739 T2740 auxpass was,confirmed
R2456 T2740 T2740 ROOT confirmed,confirmed
R2457 T2741 T2740 advcl using,confirmed
R2458 T2742 T2743 amod 2H-NMR,spectroscopy
R2459 T2743 T2741 dobj spectroscopy,using
R2460 T2744 T2740 punct .,confirmed
R2461 T2745 T2746 det The,absence
R2462 T2746 T2755 nsubjpass absence,confirmed
R2463 T2747 T2746 prep of,absence
R2464 T2748 T2749 compound LPS,contamination
R2465 T2749 T2747 pobj contamination,of
R2466 T2750 T2749 prep in,contamination
R2467 T2751 T2753 det the,fractions
R2468 T2752 T2753 nummod two,fractions
R2469 T2753 T2750 pobj fractions,in
R2470 T2754 T2755 auxpass was,confirmed
R2471 T2755 T2755 ROOT confirmed,confirmed
R2472 T2756 T2755 advcl using,confirmed
R2473 T2757 T2760 det the,kit
R2474 T2758 T2760 amod e-Toxate,kit
R2475 T2759 T2760 compound ®,kit
R2476 T2760 T2756 dobj kit,using
R2477 T2761 T2760 punct (,kit
R2478 T2762 T2760 appos Sigma,kit
R2479 T2763 T2762 punct ",",Sigma
R2480 T2764 T2766 compound St,Fallavier
R2481 T2765 T2766 compound Quentin,Fallavier
R2482 T2766 T2762 conj Fallavier,Sigma
R2483 T2767 T2766 punct ",",Fallavier
R2484 T2768 T2766 conj France,Fallavier
R2485 T2769 T2766 punct ),Fallavier
R2486 T2770 T2755 punct .,confirmed
R2487 T2771 T2781 prep Before,dissolved
R2488 T2772 T2771 pobj use,Before
R2489 T2773 T2772 prep in,use
R2490 T2774 T2775 compound cell,culture
R2491 T2775 T2773 pobj culture,in
R2492 T2776 T2781 punct ",",dissolved
R2647 T2959 T2960 auxpass were,housed
R2648 T2960 T2960 ROOT housed,housed
R2649 T2961 T2960 prep under,housed
R2650 T2962 T2963 amod standard,conditions
R2651 T2963 T2961 pobj conditions,under
R2652 T2964 T2960 cc and,housed
R2653 T2965 T2960 conj fed,housed
R2654 T2966 T2967 compound ad,libitum
R2655 T2967 T2965 dobj libitum,fed
R2656 T2968 T2967 prep with,libitum
R2657 T2969 T2972 amod standard,chow
R2658 T2970 T2972 compound rodent,chow
R2659 T2971 T2972 compound laboratory,chow
R2660 T2972 T2968 pobj chow,with
R2661 T2973 T2960 punct .,housed
R2662 T2974 T2975 amod Degraded,iota-carrageenans
R2663 T2975 T2977 nsubjpass iota-carrageenans,administered
R2664 T2976 T2977 auxpass were,administered
R2665 T2977 T2977 ROOT administered,administered
R2666 T2978 T2977 prep in,administered
R2667 T2979 T2981 det the,water
R2669 T2981 T2978 pobj water,in
R2730 T3042 T3038 punct ),water
R2731 T3043 T3034 punct .,maintained
R2732 T3044 T3045 aux To,increase
R2733 T3045 T3051 advcl increase,added
R2734 T3046 T3049 amod palatability,sucrose
R2735 T3047 T3048 nummod 0.2,%
R2736 T3048 T3049 compound %,sucrose
R2737 T3049 T3051 nsubjpass sucrose,added
R2738 T3050 T3051 auxpass was,added
R2739 T3051 T3051 ROOT added,added
R2740 T3052 T3051 prep to,added
R2741 T3053 T3055 det the,water
R2742 T3054 T3055 compound drinking,water
R2743 T3055 T3052 pobj water,to
R2744 T3056 T3055 prep of,water
R2745 T3057 T3058 det all,groups
R2746 T3058 T3056 pobj groups,of
R2747 T3059 T3062 punct (,Waaji
R2748 T3060 T3062 compound Van,Waaji
R2749 T3061 T3062 compound der,Waaji
R2750 T3062 T3064 compound Waaji,al.
R2751 T3063 T3064 compound et,al.
R2752 T3064 T3055 appos al.,water
R2753 T3065 T3055 punct ",",water
R2754 T3066 T3055 appos [,water
R2755 T3067 T3068 nummod 23,]
R2756 T3068 T3066 appos ],[
R2757 T3069 T3066 punct ),[
R2758 T3070 T3051 punct .,added
R2759 T3071 T3073 amod Fresh,solutions
R2760 T3072 T3073 compound carrageenan,solutions
R2761 T3073 T3075 nsubjpass solutions,prepared
R2762 T3074 T3075 auxpass were,prepared
R2763 T3075 T3075 ROOT prepared,prepared
R2764 T3076 T3075 advmod daily,prepared
R2765 T3077 T3075 punct .,prepared
R3012 T3368 T3390 nsubjpass Evaluation,recorded
R3013 T3369 T3368 prep of,Evaluation
R3014 T3370 T3371 compound Colitis,Body
R3015 T3371 T3372 compound Body,weight
R3016 T3372 T3369 pobj weight,of
R3017 T3373 T3372 punct ",",weight
R3018 T3374 T3372 conj liquid,weight
R3019 T3375 T3374 cc and,liquid
R3020 T3376 T3377 compound food,consumption
R3021 T3377 T3374 conj consumption,liquid
R3022 T3378 T3377 punct ",",consumption
R3023 T3379 T3377 conj diarrhea,consumption
R3024 T3380 T3379 cc and,diarrhea
R3025 T3381 T3382 amod rectal,bleeding
R3026 T3382 T3379 conj bleeding,diarrhea
R3027 T3383 T3382 punct (,bleeding
R3028 T3384 T3382 acl detected,bleeding
R3029 T3385 T3384 agent by,detected
R3030 T3386 T3387 compound eye,inspection
R3031 T3387 T3385 pobj inspection,by
R3032 T3388 T3368 punct ),Evaluation
R3033 T3389 T3390 auxpass were,recorded
R3034 T3390 T3390 ROOT recorded,recorded
R3035 T3391 T3390 prep throughout,recorded
R3036 T3392 T3394 det the,period
R3037 T3393 T3394 compound feeding,period
R3038 T3394 T3391 pobj period,throughout
R3039 T3395 T3390 punct .,recorded
R3040 T3396 T3402 prep After,sacrificed
R3041 T3397 T3398 nummod 55,days
R3042 T3398 T3396 pobj days,After
R3043 T3399 T3402 punct ",",sacrificed
R3044 T3400 T3402 nsubjpass animals,sacrificed
R3045 T3401 T3402 auxpass were,sacrificed
R3046 T3402 T3402 ROOT sacrificed,sacrificed
R3047 T3403 T3402 agent by,sacrificed
R3048 T3404 T3405 amod cervical,dislocation
R3049 T3405 T3403 pobj dislocation,by
R3050 T3406 T3402 punct .,sacrificed
R3051 T3407 T3408 det The,length
R3052 T3408 T3413 nsubjpass length,measured
R3053 T3409 T3408 prep of,length
R3054 T3410 T3411 det the,colon
R3055 T3411 T3409 pobj colon,of
R3056 T3412 T3413 auxpass was,measured
R3057 T3413 T3413 ROOT measured,measured
R3058 T3414 T3415 mark as,described
R3059 T3415 T3413 advcl described,measured
R3060 T3416 T3415 agent by,described
R3061 T3417 T3416 pobj Okayashu,by
R3062 T3418 T3422 nmod et,]
R3063 T3419 T3422 nmod al.,]
R3064 T3420 T3422 nmod [,]
R3065 T3421 T3422 nummod 24,]
R3066 T3422 T3415 npadvmod ],described
R3067 T3423 T3413 punct .,measured
R3068 T3424 T3429 advmod Then,ligated
R3069 T3425 T3429 punct ",",ligated
R3070 T3426 T3427 det each,colon
R3071 T3427 T3429 nsubjpass colon,ligated
R3072 T3428 T3429 auxpass was,ligated
R3073 T3429 T3429 ROOT ligated,ligated
R3074 T3430 T3429 prep in,ligated
R3075 T3431 T3430 pobj sections,in
R3076 T3432 T3431 prep of,sections
R3077 T3433 T3434 nummod 2,cm
R3078 T3434 T3432 pobj cm,of
R3079 T3435 T3429 cc and,ligated
R3080 T3436 T3438 quantmod 1,2
R3081 T3437 T3438 quantmod to,2
R3082 T3438 T3439 nummod 2,ml
R3083 T3439 T3445 nsubjpass ml,infused
R3084 T3440 T3439 prep of,ml
R3085 T3441 T3442 nummod 10,%
R3086 T3442 T3443 compound %,formalin
R3087 T3443 T3440 pobj formalin,of
R3088 T3444 T3445 auxpass was,infused
R3089 T3445 T3429 conj infused,ligated
R3090 T3446 T3445 prep into,infused
R3091 T3447 T3449 det the,lumen
R3092 T3448 T3449 amod intestinal,lumen
R3093 T3449 T3446 pobj lumen,into
R3094 T3450 T3445 punct .,infused
R3095 T3451 T3454 det The,segment
R3096 T3452 T3453 advmod moderately,distended
R3097 T3453 T3454 amod distended,segment
R3098 T3454 T3455 nsubj segment,was
R3099 T3455 T3455 ROOT was,was
R3100 T3456 T3455 attr sectioned,was
R3101 T3457 T3456 cc and,sectioned
R3102 T3458 T3456 conj fixed,sectioned
R3103 T3459 T3458 prep in,fixed
R3104 T3460 T3461 nummod 10,%
R3105 T3461 T3462 compound %,formalin
R3106 T3462 T3459 pobj formalin,in
R3107 T3463 T3455 punct .,was
R3108 T3464 T3466 det The,day
R3109 T3465 T3466 amod following,day
R3110 T3466 T3472 npadvmod day,removed
R3111 T3467 T3472 punct ",",removed
R3112 T3468 T3470 det the,content
R3113 T3469 T3470 amod intestinal,content
R3114 T3470 T3472 nsubjpass content,removed
R3115 T3471 T3472 auxpass was,removed
R3116 T3472 T3472 ROOT removed,removed
R3117 T3473 T3472 agent by,removed
R3118 T3474 T3473 pobj vortexing,by
R3119 T3475 T3472 punct .,removed
R3120 T3476 T3478 det The,segment
R3121 T3477 T3478 amod fixed,segment
R3122 T3478 T3480 nsubjpass segment,kept
R3123 T3479 T3480 auxpass was,kept
R3124 T3480 T3480 ROOT kept,kept
R3125 T3481 T3480 oprd in,kept
R3126 T3482 T3483 nummod 10,%
R3127 T3483 T3484 compound %,formalin
R3128 T3484 T3481 pobj formalin,in
R3129 T3485 T3480 prep at,kept
R3130 T3486 T3487 nummod 4,°
R3131 T3487 T3488 nummod °,C
R3132 T3488 T3485 pobj C,at
R3133 T3489 T3480 prep until,kept
R3134 T3490 T3493 det the,procedure
R3135 T3491 T3492 compound paraffin,embedding
R3136 T3492 T3493 compound embedding,procedure
R3137 T3493 T3489 pobj procedure,until
R3138 T3494 T3480 punct .,kept
R3139 T3495 T3496 aux To,evaluate
R3140 T3496 T3507 advcl evaluate,opened
R3141 T3497 T3498 det the,degree
R3142 T3498 T3496 dobj degree,evaluate
R3143 T3499 T3498 prep of,degree
R3144 T3500 T3499 pobj inflammation,of
R3145 T3501 T3507 punct ",",opened
R3146 T3502 T3503 det this,segment
R3161 T3517 T3507 conj recorded,opened
R3162 T3518 T3520 mark as,described
R3163 T3519 T3520 advmod previously,described
R3164 T3520 T3517 advcl described,recorded
R3165 T3521 T3523 nmod [,]
R3166 T3522 T3523 nummod 25,]
R3167 T3523 T3520 dobj ],described
R3168 T3524 T3523 punct ",",]
R3169 T3525 T3527 nmod [,]
R3170 T3526 T3527 nummod 26,]
R3171 T3527 T3523 appos ],]
R3172 T3528 T3517 punct .,recorded
R3173 T3529 T3532 det The,staining
R3174 T3530 T3532 nmod toluidine,staining
R3175 T3531 T3532 amod blue,staining
R3176 T3532 T3534 nsubjpass staining,used
R3177 T3533 T3534 auxpass was,used
R3178 T3534 T3534 ROOT used,used
R3179 T3535 T3534 prep for,used
R3180 T3536 T3535 pobj identification,for
R3181 T3537 T3536 prep of,identification
R3182 T3538 T3539 amod sulphated,polysaccharides
R3183 T3539 T3537 pobj polysaccharides,of
R3184 T3540 T3539 prep in,polysaccharides
R3185 T3541 T3543 det the,mucosa
R3186 T3542 T3543 amod intestinal,mucosa
R3187 T3543 T3540 pobj mucosa,in
R3188 T3544 T3534 punct .,used
R3189 T3545 T3562 prep On,collected
R3190 T3546 T3547 det the,day
R3191 T3547 T3545 pobj day,On
R3192 T3548 T3547 prep of,day
R3193 T3549 T3548 pobj sacrifice,of
R3194 T3550 T3562 punct ",",collected
R3195 T3551 T3553 det a,sample
R3196 T3552 T3553 amod fresh,sample
R3197 T3553 T3562 nsubjpass sample,collected
R3198 T3554 T3553 prep of,sample
R3199 T3555 T3556 det each,colon
R3200 T3556 T3554 pobj colon,of
R3201 T3557 T3556 punct (,colon
R3202 T3558 T3559 nummod 50,mg
R3203 T3559 T3556 appos mg,colon
R3204 T3560 T3556 punct ),colon
R3205 T3561 T3562 auxpass was,collected
R3206 T3562 T3562 ROOT collected,collected
R3207 T3563 T3562 prep for,collected
R3208 T3564 T3563 pobj myeloperoxidase,for
R3209 T3565 T3566 punct (,MPO
R3210 T3566 T3564 appos MPO,myeloperoxidase
R3211 T3567 T3566 punct ),MPO
R3212 T3568 T3569 amod assay,according
R3213 T3569 T3562 prep according,collected
R3214 T3570 T3569 prep to,according
R3215 T3571 T3573 compound Krawisz,al.
R3216 T3572 T3573 nmod et,al.
R3217 T3573 T3570 pobj al.,to
R3218 T3574 T3573 punct ",",al.
R3219 T3575 T3577 nmod [,]
R3220 T3576 T3577 nummod 27,]
R3221 T3577 T3573 appos ],al.
R3222 T3578 T3562 punct .,collected
R3223 T3579 T3580 det The,level
R3224 T3580 T3589 nsubj level,indicates
R3234 T3590 T3591 det the,rate
R3235 T3591 T3589 dobj rate,indicates
R3236 T3592 T3591 prep of,rate
R3237 T3593 T3592 pobj recruitment,of
R3238 T3594 T3593 prep of,recruitment
R3239 T3595 T3594 pobj neutrophils,of
R3240 T3596 T3589 prep to,indicates
R3241 T3597 T3599 det the,mucosa
R3242 T3598 T3599 amod intestinal,mucosa
R3243 T3599 T3596 pobj mucosa,to
R3244 T3600 T3589 punct .,indicates
R3245 T3601 T3602 nummod One,unit
R3246 T3602 T3606 nsubj unit,corresponds
R3247 T3603 T3602 prep of,unit
R3248 T3604 T3605 compound MPO,activity
R3249 T3605 T3603 pobj activity,of
R3250 T3606 T3606 ROOT corresponds,corresponds
R3251 T3607 T3606 prep to,corresponds
R3252 T3608 T3609 det the,degradation
R3253 T3609 T3607 pobj degradation,to
R3254 T3610 T3609 prep of,degradation
R3255 T3611 T3612 nummod 1,µmol
R3256 T3612 T3610 pobj µmol,of
R3257 T3613 T3612 prep of,µmol
R3258 T3614 T3613 pobj peroxide,of
R3259 T3615 T3614 prep per,peroxide
R3260 T3616 T3615 pobj minute,per
R3261 T3617 T3609 prep at,degradation
R3262 T3618 T3619 nummod 25,°
R3263 T3619 T3620 nummod °,C.
R3264 T3620 T3617 pobj C.,at
R3413 T3816 T3818 compound Cell,All
R3414 T3817 T3818 compound Culture,All
R3415 T3818 T3821 det All,reagents
R3416 T3819 T3820 compound tissue,culture
R3417 T3820 T3821 compound culture,reagents
R3418 T3821 T3822 nsubj reagents,were
R3419 T3822 T3822 ROOT were,were
R3420 T3823 T3822 prep from,were
R3421 T3824 T3823 pobj Invitrogen,from
R3422 T3825 T3824 punct (,Invitrogen
R3423 T3826 T3827 compound Cergy,Pontoise
R3424 T3827 T3824 appos Pontoise,Invitrogen
R3425 T3828 T3827 punct ",",Pontoise
R3426 T3829 T3827 npadvmod France,Pontoise
R3427 T3830 T3824 punct ),Invitrogen
R3428 T3831 T3822 punct .,were
R3429 T3832 T3835 nummod THP-1,cells
R3430 T3833 T3835 amod human,cells
R3431 T3834 T3835 amod monocytic,cells
R3432 T3835 T3837 nsubjpass cells,maintained
R3433 T3836 T3837 auxpass were,maintained
R3434 T3837 T3837 ROOT maintained,maintained
R3435 T3838 T3837 prep in,maintained
R3436 T3839 T3838 pobj RPMI-1640,in
R3437 T3840 T3837 conj supplemented,maintained
R3438 T3841 T3840 prep with,supplemented
R3439 T3842 T3843 nummod 10,%
R3440 T3843 T3844 compound %,FCS
R3441 T3844 T3841 pobj FCS,with
R3442 T3845 T3844 punct ",",FCS
R3443 T3846 T3850 nummod 2,glutamine
R3444 T3847 T3850 compound mM,glutamine
R3445 T3848 T3850 compound L,glutamine
R3446 T3849 T3850 punct -,glutamine
R3447 T3850 T3844 appos glutamine,FCS
R3448 T3851 T3850 punct ",",glutamine
R3449 T3852 T3854 nummod 50,penicillin
R3450 T3853 T3854 compound U/ml,penicillin
R3451 T3854 T3862 nsubj penicillin,C
R3452 T3855 T3854 cc and,penicillin
R3453 T3856 T3857 nummod 50,mg/ml
R3454 T3857 T3858 compound mg/ml,streptomycin
R3455 T3858 T3854 conj streptomycin,penicillin
R3456 T3859 T3854 prep at,penicillin
R3457 T3860 T3861 nummod 37,°
R3458 T3861 T3859 pobj °,at
R3459 T3862 T3844 conj C,FCS
R3460 T3863 T3837 prep in,maintained
R3461 T3864 T3868 det a,incubator
R3462 T3865 T3866 nummod 5,%
R3463 T3866 T3868 nmod %,incubator
R3464 T3867 T3868 nummod CO2,incubator
R3465 T3868 T3863 pobj incubator,in
R3466 T3869 T3837 punct .,maintained
R3467 T3870 T3874 amod Human,cells
R3468 T3871 T3874 amod peripheral,cells
R3469 T3872 T3874 compound blood,cells
R3470 T3873 T3874 compound mononuclear,cells
R3471 T3874 T3876 nsubjpass cells,obtained
R3472 T3875 T3876 auxpass were,obtained
R3473 T3876 T3876 ROOT obtained,obtained
R3474 T3877 T3876 prep from,obtained
R3475 T3878 T3879 amod heparinized,blood
R3476 T3879 T3877 pobj blood,from
R3477 T3880 T3876 agent by,obtained
R3478 T3881 T3883 amod Ficoll-Hypaque,gradient
R3479 T3882 T3883 compound density,gradient
R3480 T3883 T3880 pobj gradient,by
R3481 T3884 T3876 punct .,obtained
R3482 T3885 T3888 nsubjpass Monocytes,isolated
R3483 T3886 T3888 auxpass were,isolated
R3484 T3887 T3888 advmod then,isolated
R3485 T3888 T3888 ROOT isolated,isolated
R3486 T3889 T3888 agent by,isolated
R3487 T3890 T3889 pobj adherence,by
R3488 T3891 T3890 prep to,adherence
R3489 T3892 T3893 compound culture,flasks
R3490 T3893 T3891 pobj flasks,to
R3491 T3894 T3895 mark as,described
R3492 T3895 T3888 advcl described,isolated
R3493 T3896 T3898 compound [,]
R3494 T3897 T3898 compound 28,]
R3495 T3898 T3895 dobj ],described
R3496 T3899 T3888 punct .,isolated
R3497 T3900 T3906 prep For,cultured
R3498 T3901 T3902 compound cell,aggregation
R3499 T3902 T3900 pobj aggregation,For
R3500 T3903 T3906 punct ",",cultured
R3501 T3904 T3906 nsubjpass monocytes,cultured
R3502 T3905 T3906 auxpass were,cultured
R3503 T3906 T3906 ROOT cultured,cultured
R3504 T3907 T3906 prep in,cultured
R3505 T3908 T3909 det the,presence
R3506 T3909 T3907 pobj presence,in
R3507 T3910 T3909 cc or,presence
R3508 T3911 T3909 conj absence,presence
R3509 T3912 T3911 prep of,absence
R3510 T3913 T3912 pobj C10,of
R3511 T3914 T3913 cc or,C10
R3512 T3915 T3913 conj C40,C10
R3513 T3916 T3906 prep for,cultured
R3514 T3917 T3920 nummod 72,colonies
R3515 T3918 T3920 compound h.,colonies
R3516 T3919 T3920 compound Cell,colonies
R3517 T3920 T3916 pobj colonies,for
R3518 T3921 T3922 auxpass were,monitored
R3519 T3922 T3906 conj monitored,cultured
R3520 T3923 T3922 prep under,monitored
R3521 T3924 T3928 det an,microscope
R3522 T3925 T3926 amod inverted,phase
R3523 T3926 T3928 compound phase,microscope
R3524 T3927 T3928 compound contrast,microscope
R3525 T3928 T3923 pobj microscope,under
R3526 T3929 T3928 acl coupled,microscope
R3527 T3930 T3929 prep through,coupled
R3528 T3931 T3933 det a,camera
R3529 T3932 T3933 compound video,camera
R3530 T3933 T3930 pobj camera,through
R3531 T3934 T3929 prep to,coupled
R3532 T3935 T3936 det a,computer
R3533 T3936 T3934 pobj computer,to
R3534 T3937 T3906 punct .,cultured
R3535 T3938 T3964 prep In,added
R3536 T3939 T3940 det some,wells
R3537 T3940 T3938 pobj wells,In
R3538 T3941 T3964 punct ",",added
R3539 T3942 T3964 csubj neutralizing,added
R3540 T3943 T3944 amod monoclonal,antibody
R3541 T3944 T3942 dobj antibody,neutralizing
R3542 T3945 T3942 prep to,neutralizing
R3543 T3946 T3945 pobj ICAM-1,to
R3544 T3947 T3951 punct (,ml
R3545 T3948 T3949 nummod 2.5,µg
R3546 T3949 T3951 nmod µg,ml
R3547 T3950 T3951 compound /,ml
R3548 T3951 T3946 appos ml,ICAM-1
R3549 T3952 T3946 punct ),ICAM-1
R3550 T3953 T3964 punct (,added
R3551 T3954 T3964 dep Tebu,added
R3552 T3955 T3954 punct ",",Tebu
R3553 T3956 T3957 compound Le,Perray
R3554 T3957 T3964 nsubjpass Perray,added
R3555 T3958 T3957 prep en,Perray
R3556 T3959 T3958 pobj Yvelines,en
R3557 T3960 T3959 punct ",",Yvelines
R3558 T3961 T3959 appos France,Yvelines
R3559 T3962 T3959 punct ),Yvelines
R3560 T3963 T3964 auxpass was,added
R3561 T3964 T3964 ROOT added,added
R3562 T3965 T3964 punct .,added
R3680 T4100 T4102 compound Cell,Analysis
R3681 T4101 T4102 compound Cycle,Analysis
R3682 T4102 T4104 compound Analysis,cells
R3683 T4103 T4104 compound THP-1,cells
R3684 T4104 T4110 nsubjpass cells,exposed
R3685 T4105 T4104 prep in,cells
R3686 T4106 T4108 amod exponential,phase
R3687 T4107 T4108 compound growth,phase
R3688 T4108 T4105 pobj phase,in
R3689 T4109 T4110 auxpass were,exposed
R3690 T4110 T4110 ROOT exposed,exposed
R3691 T4111 T4112 aux to,complete
R3692 T4112 T4110 xcomp complete,exposed
R3693 T4113 T4112 dobj medium,complete
R3694 T4114 T4112 prep in,complete
R3695 T4115 T4116 det the,presence
R3696 T4116 T4114 pobj presence,in
R3697 T4117 T4116 cc or,presence
R3698 T4118 T4116 conj absence,presence
R3699 T4119 T4118 prep of,absence
R3700 T4120 T4119 pobj carrageenans,of
R3701 T4121 T4112 prep for,complete
R3702 T4122 T4123 nummod 24,h
R3703 T4123 T4121 pobj h,for
R3704 T4124 T4112 prep before,complete
R3705 T4125 T4126 auxpass being,stained
R3706 T4126 T4124 pcomp stained,before
R3707 T4127 T4126 prep with,stained
R3708 T4128 T4129 compound propidium,iodide
R3709 T4129 T4127 pobj iodide,with
R3710 T4130 T4124 pcomp using,before
R3711 T4131 T4134 det the,kit
R3712 T4132 T4133 compound DNA-Prep,Coulter
R3713 T4133 T4134 compound Coulter,kit
R3714 T4134 T4130 dobj kit,using
R3715 T4135 T4110 prep according,exposed
R3716 T4136 T4135 prep to,according
R3717 T4137 T4138 det the,manufacturer
R3718 T4138 T4140 poss manufacturer,instruction
R3719 T4139 T4138 case 's,manufacturer
R3720 T4140 T4136 pobj instruction,to
R3721 T4141 T4142 punct (,Beckman-Coulter
R3722 T4142 T4140 appos Beckman-Coulter,instruction
R3723 T4143 T4142 punct ",",Beckman-Coulter
R3724 T4144 T4142 conj Villepinte,Beckman-Coulter
R3725 T4145 T4144 punct ",",Villepinte
R3726 T4146 T4144 conj France,Villepinte
R3727 T4147 T4110 punct ),exposed
R3728 T4148 T4110 punct .,exposed
R3729 T4149 T4150 compound Cell,DNA
R3730 T4150 T4151 compound DNA,content
R3731 T4151 T4154 nsubjpass content,analyzed
R3732 T4152 T4154 auxpass was,analyzed
R3733 T4153 T4154 advmod then,analyzed
R3734 T4154 T4154 ROOT analyzed,analyzed
R3735 T4155 T4154 agent by,analyzed
R3736 T4156 T4157 compound flow,cytometry
R3737 T4157 T4155 pobj cytometry,by
R3738 T4158 T4154 advcl using,analyzed
R3739 T4159 T4161 det an,XL2
R3740 T4160 T4161 compound EPICS,XL2
R3741 T4161 T4158 dobj XL2,using
R3742 T4162 T4163 punct (,Beckman-Coulter
R3743 T4163 T4161 appos Beckman-Coulter,XL2
R3744 T4164 T4161 punct ),XL2
R3745 T4165 T4154 punct .,analyzed
R3746 T4166 T4167 amod Raw,data
R3747 T4167 T4182 nsubjpass data,expressed
R3748 T4168 T4167 prep for,data
R3749 T4169 T4170 det the,distribution
R3750 T4170 T4168 pobj distribution,for
R3751 T4171 T4170 prep of,distribution
R3752 T4172 T4173 compound DNA,content
R3753 T4173 T4171 pobj content,of
R3754 T4174 T4173 prep of,content
R3755 T4175 T4176 nummod "30,000",cells
R3756 T4176 T4174 pobj cells,of
R3757 T4177 T4170 acl retrieved,distribution
R3758 T4178 T4177 prep from,retrieved
R3759 T4179 T4180 det the,cytometer
R3760 T4180 T4178 pobj cytometer,from
R3761 T4181 T4182 auxpass were,expressed
R3762 T4182 T4182 ROOT expressed,expressed
R3763 T4183 T4182 prep as,expressed
R3764 T4184 T4185 det the,percentage
R3765 T4185 T4183 pobj percentage,as
R3766 T4186 T4185 prep of,percentage
R3767 T4187 T4186 pobj G0/G1,of
R3768 T4188 T4182 prep through,expressed
R3769 T4189 T4190 compound G2/M,populations
R3770 T4190 T4188 pobj populations,through
R3771 T4191 T4182 punct .,expressed
R3772 T4192 T4194 amod Multicycle,software
R3773 T4193 T4194 compound AV,software
R3774 T4194 T4206 nsubjpass software,used
R3775 T4195 T4198 punct (,Systems
R3776 T4196 T4198 compound Phoenix,Systems
R3777 T4197 T4198 compound Flow,Systems
R3778 T4198 T4194 appos Systems,software
R3779 T4199 T4198 punct ",",Systems
R3780 T4200 T4201 compound San,Diego
R3781 T4201 T4198 npadvmod Diego,Systems
R3782 T4202 T4201 punct ",",Diego
R3783 T4203 T4201 conj CA,Diego
R3784 T4204 T4198 punct ),Systems
R3785 T4205 T4206 auxpass was,used
R3786 T4206 T4206 ROOT used,used
R3787 T4207 T4208 aux to,generate
R3788 T4208 T4206 xcomp generate,used
R3789 T4209 T4212 compound DNA,histograms
R3790 T4210 T4211 amod content,frequency
R3791 T4211 T4212 compound frequency,histograms
R3792 T4212 T4208 dobj histograms,generate
R3793 T4213 T4208 cc and,generate
R3794 T4214 T4208 conj facilitate,generate
R3795 T4215 T4216 compound data,analysis
R3796 T4216 T4214 dobj analysis,facilitate
R3797 T4217 T4206 punct .,used
R3936 T4382 T4384 compound Cell,Antigen
R3937 T4383 T4384 compound Surface,Antigen
R3938 T4384 T4389 compound Antigen,Monocytes
R3939 T4385 T4386 compound Expression,Analysis
R3953 T4399 T4400 det the,presence
R3954 T4400 T4398 pobj presence,in
R3955 T4401 T4400 cc or,presence
R3956 T4402 T4400 conj absence,presence
R3957 T4403 T4402 prep of,absence
R3958 T4404 T4403 pobj carrageenan,of
R3959 T4405 T4396 prep for,complete
R3960 T4406 T4407 nummod 36,h
R3961 T4407 T4405 pobj h,for
R3962 T4408 T4394 punct .,exposed
R3963 T4409 T4423 prep After,incubated
R3964 T4410 T4411 nummod two,washes
R3965 T4411 T4409 pobj washes,After
R3966 T4412 T4411 prep in,washes
R3967 T4413 T4412 pobj PBS,in
R3968 T4414 T4411 prep without,washes
R3969 T4415 T4416 compound Ca2,+
R3970 T4416 T4414 pobj +,without
R3971 T4417 T4416 cc and,+
R3972 T4418 T4419 compound Mg2,+
R3973 T4419 T4416 conj +,+
R3974 T4420 T4423 punct ",",incubated
R3975 T4421 T4423 nsubjpass cells,incubated
R3976 T4422 T4423 auxpass were,incubated
R3977 T4423 T4423 ROOT incubated,incubated
R3978 T4424 T4423 prep in,incubated
R3979 T4425 T4424 pobj PBS,in
R3980 T4426 T4423 advcl containing,incubated
R3981 T4427 T4428 nummod 0.1,%
R3982 T4428 T4429 compound %,gelatin
R3983 T4429 T4426 dobj gelatin,containing
R3984 T4430 T4429 cc and,gelatin
R3985 T4431 T4432 nummod 8,%
R3986 T4432 T4429 conj %,gelatin
R3987 T4433 T4435 nmod AB,serum
R3988 T4434 T4435 amod human,serum
R3989 T4435 T4429 conj serum,gelatin
R3990 T4436 T4437 aux to,prevent
R3991 T4437 T4435 relcl prevent,serum
R3992 T4438 T4437 advmod binding,prevent
R3993 T4439 T4438 prep to,binding
R3994 T4440 T4441 compound Fc,receptors
R3995 T4441 T4439 pobj receptors,to
R3996 T4442 T4423 punct .,incubated
R3997 T4443 T4450 advmod Then,incubated
R3998 T4444 T4450 punct ",",incubated
R3999 T4445 T4448 nummod 5,cells
R4000 T4446 T4448 nmod ×,cells
R4001 T4447 T4448 nummod 105,cells
R4002 T4448 T4450 nsubjpass cells,incubated
R4003 T4449 T4450 auxpass were,incubated
R4004 T4450 T4450 ROOT incubated,incubated
R4005 T4451 T4450 prep with,incubated
R4006 T4452 T4453 amod primary,antibodies
R4007 T4453 T4451 pobj antibodies,with
R4008 T4454 T4450 prep at,incubated
R4009 T4455 T4456 nummod 4,°
R4010 T4456 T4454 pobj °,at
R4011 T4457 T4450 conj C,incubated
R4012 T4458 T4457 prep for,C
R4013 T4459 T4460 nummod 30,min
R4014 T4460 T4458 pobj min,for
R4015 T4461 T4450 punct .,incubated
R4016 T4462 T4464 nummod Two,washes
R4017 T4463 T4464 amod other,washes
R4018 T4464 T4467 nsubj washes,preceded
R4019 T4465 T4464 prep in,washes
R4020 T4466 T4465 pobj PBS,in
R4021 T4467 T4467 ROOT preceded,preceded
R4022 T4468 T4467 dobj incubation,preceded
R4023 T4469 T4467 prep with,preceded
R4024 T4470 T4475 advmod FITC-conjugated,diluted
R4025 T4471 T4472 compound goat,antibody
R4026 T4472 T4475 nsubj antibody,diluted
R4027 T4473 T4474 compound anti-mouse,IgG
R4028 T4474 T4472 appos IgG,antibody
R4029 T4475 T4469 pcomp diluted,with
R4030 T4476 T4475 dobj 1/1000,diluted
R4031 T4477 T4475 prep at,diluted
R4032 T4478 T4479 nummod 4,°
R4033 T4479 T4477 pobj °,at
R4034 T4480 T4475 npadvmod C,diluted
R4035 T4481 T4475 prep for,diluted
R4036 T4482 T4483 nummod 30,min
R4037 T4483 T4481 pobj min,for
R4038 T4484 T4483 punct (,min
R4039 T4485 T4483 appos Tebu,min
R4040 T4486 T4483 punct ),min
R4041 T4487 T4467 punct .,preceded
R4042 T4488 T4498 mark After,performed
R4043 T4489 T4491 nummod two,washes
R4044 T4490 T4491 amod additional,washes
R4045 T4491 T4488 pobj washes,After
R4046 T4492 T4491 punct ",",washes
R4047 T4493 T4491 conj analysis,washes
R4048 T4494 T4493 prep of,analysis
R4049 T4495 T4496 amod stained,cells
R4050 T4496 T4494 pobj cells,of
R4051 T4497 T4498 auxpass was,performed
R4052 T4498 T4498 ROOT performed,performed
R4053 T4499 T4498 prep on,performed
R4054 T4500 T4502 det an,XL2
R4055 T4501 T4502 compound EPICS,XL2
R4056 T4502 T4499 pobj XL2,on
R4057 T4503 T4502 punct (,XL2
R4058 T4504 T4502 appos Beckman-Coulter,XL2
R4059 T4505 T4502 punct ),XL2
R4060 T4506 T4498 punct .,performed
R4061 T4507 T4509 det The,population
R4062 T4508 T4509 compound cell,population
R4063 T4509 T4511 nsubjpass population,gated
R4064 T4510 T4511 auxpass was,gated
R4065 T4511 T4511 ROOT gated,gated
R4066 T4512 T4511 prep according,gated
R4067 T4513 T4512 prep to,according
R4068 T4514 T4519 poss its,scattering
R4069 T4515 T4519 amod forward,scattering
R4070 T4516 T4515 cc and,forward
R4071 T4517 T4515 conj wide-angle,forward
R4072 T4518 T4519 amod light,scattering
R4073 T4519 T4513 pobj scattering,to
R4074 T4520 T4511 punct .,gated
R4075 T4521 T4523 nsubjpass Data,expressed
R4076 T4522 T4523 auxpass were,expressed
R4077 T4523 T4523 ROOT expressed,expressed
R4078 T4524 T4523 prep as,expressed
R4079 T4525 T4528 amod mean,intensity
R4080 T4526 T4528 amod relative,intensity
R4081 T4527 T4528 compound fluorescence,intensity
R4082 T4528 T4524 pobj intensity,as
R4083 T4529 T4530 punct (,MFI
R4084 T4530 T4528 appos MFI,intensity
R4085 T4531 T4530 punct ),MFI
R4086 T4532 T4528 prep of,intensity
R4087 T4533 T4534 nummod 3000,cells
R4088 T4534 T4532 pobj cells,of
R4089 T4535 T4523 punct .,expressed
R4150 T4648 T4651 compound TNF,Monocytes
R4151 T4649 T4651 compound Activity,Monocytes
R4152 T4650 T4651 compound Bioassay,Monocytes
R4153 T4651 T4656 nsubjpass Monocytes,cultured
R4154 T4652 T4651 cc or,Monocytes
R4155 T4653 T4654 nummod THP-1,cells
R4156 T4654 T4651 conj cells,Monocytes
R4157 T4655 T4656 auxpass were,cultured
R4158 T4656 T4656 ROOT cultured,cultured
R4159 T4657 T4656 prep with,cultured
R4160 T4658 T4657 cc or,with
R4161 T4659 T4657 conj without,with
R4162 T4660 T4661 amod different,concentrations
R4163 T4661 T4659 pobj concentrations,without
R4164 T4662 T4661 prep of,concentrations
R4165 T4663 T4662 pobj CGNs,of
R4166 T4664 T4663 cc or,CGNs
R4167 T4665 T4663 conj LPS,CGNs
R4168 T4666 T4668 punct (,typhosa
R4169 T4667 T4668 compound Salmonella,typhosa
R4170 T4668 T4665 appos typhosa,LPS
R4171 T4669 T4668 punct ",",typhosa
R4172 T4670 T4668 npadvmod Sigma,typhosa
R4173 T4671 T4668 punct ),typhosa
R4174 T4672 T4665 prep for,LPS
R4175 T4673 T4674 nummod 24,h
R4176 T4674 T4672 pobj h,for
R4177 T4675 T4674 cc or,h
R4178 T4676 T4678 det the,time
R4179 T4677 T4678 amod indicated,time
R4180 T4678 T4674 conj time,h
R4181 T4679 T4656 punct .,cultured
R4182 T4680 T4684 advmod Biologically,β
R4183 T4681 T4684 amod active,β
R4184 T4682 T4684 compound TNF-α,β
R4185 T4683 T4684 compound /,β
R4186 T4684 T4690 nsubjpass β,measured
R4187 T4685 T4684 prep in,β
R4188 T4686 T4687 compound tissue,culture
R4189 T4687 T4685 pobj culture,in
R4190 T4688 T4690 nsubjpass supernatant,measured
R4191 T4689 T4690 auxpass was,measured
R4192 T4690 T4690 ROOT measured,measured
R4193 T4691 T4690 advcl using,measured
R4194 T4692 T4698 det the,assay
R4195 T4693 T4696 nmod WEHI,13-cell
R4196 T4694 T4693 nummod 164,WEHI
R4197 T4695 T4696 compound clone,13-cell
R4198 T4696 T4698 compound 13-cell,assay
R4199 T4697 T4698 compound killing,assay
R4200 T4698 T4691 dobj assay,using
R4201 T4699 T4701 nmod [,]
R4202 T4700 T4701 nummod 29,]
R4203 T4701 T4698 appos ],assay
R4204 T4702 T4690 punct .,measured
R4205 T4703 T4704 compound TNF,concentrations
R4206 T4704 T4706 nsubjpass concentrations,expressed
R4207 T4705 T4706 auxpass are,expressed
R4208 T4706 T4706 ROOT expressed,expressed
R4209 T4707 T4706 prep as,expressed
R4210 T4708 T4707 pobj pg/ml,as
R4211 T4709 T4706 punct .,expressed
R4414 T4951 T4952 compound RT-PCR,Analysis
R4415 T4952 T4958 nsubjpass Analysis,isolated
R4416 T4953 T4954 compound Total,RNA
R4417 T4954 T4952 appos RNA,Analysis
R4418 T4955 T4954 prep from,RNA
R4419 T4956 T4955 pobj monocytes,from
R4420 T4957 T4958 auxpass was,isolated
R4421 T4958 T4958 ROOT isolated,isolated
R4422 T4959 T4958 advcl using,isolated
R4423 T4960 T4962 compound TRIzol,™
R4424 T4961 T4962 compound Reagent,™
R4425 T4962 T4959 dobj ™,using
R4426 T4963 T4964 punct (,Invitrogen
R4427 T4964 T4962 appos Invitrogen,™
R4428 T4965 T4962 punct ),™
R4429 T4966 T4958 punct .,isolated
R4430 T4967 T4969 nsubjpass cDNA,generated
R4431 T4968 T4969 auxpass was,generated
R4432 T4969 T4969 ROOT generated,generated
R4433 T4970 T4969 prep on,generated
R4434 T4971 T4972 nummod 1,µg
R4435 T4972 T4970 pobj µg,on
R4436 T4973 T4972 prep of,µg
R4437 T4974 T4975 amod total,RNA
R4438 T4975 T4973 pobj RNA,of
R4439 T4976 T4969 prep in,generated
R4440 T4977 T4979 det a,volume
R4441 T4978 T4979 compound reaction,volume
R4442 T4979 T4976 pobj volume,in
R4443 T4980 T4979 prep of,volume
R4444 T4981 T4982 nummod 20,µl
R4445 T4982 T4980 pobj µl,of
R4446 T4983 T4969 punct ",",generated
R4447 T4984 T4969 advcl using,generated
R4448 T4985 T4986 compound M-MLV,reverse
R4449 T4986 T4984 dobj reverse,using
R4450 T4987 T4986 npadvmod transcriptase,reverse
R4451 T4988 T4987 punct (,transcriptase
R4452 T4989 T4987 appos Invitrogen,transcriptase
R4453 T4990 T4987 punct ),transcriptase
R4454 T4991 T4969 punct .,generated
R4455 T4992 T4994 nsubjpass PCR,done
R4456 T4993 T4994 auxpass was,done
R4457 T4994 T4994 ROOT done,done
R4458 T4995 T4994 prep in,done
R4459 T4996 T4998 det the,range
R4460 T4997 T4998 amod linear,range
R4461 T4998 T4995 pobj range,in
R4462 T4999 T4998 prep of,range
R4463 T5000 T4999 pobj amplification,of
R4464 T5001 T4998 punct (,range
R4465 T5002 T4994 conj determined,done
R4466 T5003 T5002 prep for,determined
R4467 T5004 T5007 det each,combination
R4468 T5005 T5006 compound primer,pair-cDNA
R4469 T5006 T5007 compound pair-cDNA,combination
R4470 T5007 T5003 pobj combination,for
R4471 T5008 T5002 punct ),determined
R4472 T5009 T4994 punct .,done
R4473 T5010 T5012 amod Standard,reactions
R4474 T5011 T5012 compound PCR,reactions
R4475 T5012 T5014 nsubjpass reactions,performed
R4476 T5013 T5014 auxpass were,performed
R4477 T5014 T5014 ROOT performed,performed
R4478 T5015 T5014 prep with,performed
R4479 T5016 T5017 nummod 1,µl
R4480 T5017 T5015 pobj µl,with
R4481 T5018 T5017 prep of,µl
R4482 T5019 T5021 det the,solution
R4483 T5020 T5021 compound cDNA,solution
R4484 T5021 T5018 pobj solution,of
R4485 T5022 T5021 punct ",",solution
R4486 T5023 T5024 nummod 50,µM
R4487 T5024 T5021 appos µM,solution
R4488 T5025 T5024 prep of,µM
R4489 T5026 T5028 det each,solution
R4490 T5027 T5028 compound primer,solution
R4491 T5028 T5025 pobj solution,of
R4492 T5029 T5024 punct ",",µM
R4493 T5030 T5031 nummod 10,mM
R4494 T5031 T5024 appos mM,µM
R4495 T5032 T5031 prep of,mM
R4496 T5033 T5034 det each,dNTP
R4497 T5034 T5032 pobj dNTP,of
R4498 T5035 T5024 punct ",",µM
R4499 T5036 T5038 nummod 25,MgCl2
R4500 T5037 T5038 compound mM,MgCl2
R4501 T5038 T5024 appos MgCl2,µM
R4502 T5039 T5038 punct ",",MgCl2
R4503 T5040 T5042 compound 10X,DNA
R4504 T5041 T5042 compound Goldstar,DNA
R4505 T5042 T5045 compound DNA,buffer
R4506 T5043 T5045 compound polymerase,buffer
R4507 T5044 T5045 compound reaction,buffer
R4508 T5045 T5038 appos buffer,MgCl2
R4509 T5046 T5038 punct ",",MgCl2
R4510 T5047 T5038 cc and,MgCl2
R4511 T5048 T5049 nummod 0.5,units
R4512 T5049 T5024 appos units,µM
R4513 T5050 T5049 prep of,units
R4514 T5051 T5053 compound Goldstar,polymerase
R4515 T5052 T5053 compound DNA,polymerase
R4516 T5053 T5050 pobj polymerase,of
R4517 T5054 T5055 punct (,Eurogentec
R4518 T5055 T5053 appos Eurogentec,polymerase
R4519 T5056 T5055 punct ",",Eurogentec
R4520 T5057 T5055 conj Seraing,Eurogentec
R4521 T5058 T5057 punct ",",Seraing
R4522 T5059 T5057 conj Belgium,Seraing
R4523 T5060 T5024 punct ),µM
R4524 T5061 T5014 punct .,performed
R4525 T5062 T5064 amod First,cycle
R4526 T5063 T5064 compound PCR,cycle
R4527 T5064 T5065 nsubj cycle,consisted
R4528 T5065 T5092 ccomp consisted,consisted
R4529 T5066 T5065 prep of,consisted
R4530 T5067 T5068 nummod 1,min
R4531 T5068 T5066 pobj min,of
R4532 T5069 T5065 prep at,consisted
R4533 T5070 T5071 nummod 92,°
R4534 T5071 T5069 pobj °,at
R4535 T5072 T5065 npadvmod C,consisted
R4536 T5073 T5072 punct ",",C
R4537 T5074 T5075 nummod 1,min
R4538 T5075 T5072 appos min,C
R4539 T5076 T5075 prep at,min
R4540 T5077 T5078 nummod 58,°
R4541 T5078 T5079 nummod °,C
R4542 T5079 T5076 pobj C,at
R4543 T5080 T5075 cc and,min
R4544 T5081 T5082 nummod 1,min
R4545 T5082 T5075 conj min,min
R4546 T5083 T5082 prep at,min
R4547 T5084 T5085 nummod 72,°
R4548 T5085 T5086 nummod °,C
R4549 T5086 T5083 pobj C,at
R4550 T5087 T5092 punct ;,consisted
R4551 T5088 T5092 advmod then,consisted
R4552 T5089 T5091 det each,cycle
R4553 T5090 T5091 compound PCR,cycle
R4554 T5091 T5092 nsubj cycle,consisted
R4555 T5092 T5092 ROOT consisted,consisted
R4556 T5093 T5092 prep of,consisted
R4557 T5094 T5095 nummod 40,sec
R4558 T5095 T5093 pobj sec,of
R4559 T5096 T5092 prep at,consisted
R4560 T5097 T5098 nummod 92,°
R4561 T5098 T5096 pobj °,at
R4562 T5099 T5092 dobj C,consisted
R4563 T5100 T5099 punct ",",C
R4564 T5101 T5102 nummod 40,sec
R4565 T5102 T5099 appos sec,C
R4566 T5103 T5102 prep at,sec
R4567 T5104 T5105 nummod 58,°
R4568 T5105 T5106 nummod °,C
R4569 T5106 T5103 pobj C,at
R4570 T5107 T5102 cc and,sec
R4571 T5108 T5109 nummod 50,sec
R4572 T5109 T5102 conj sec,sec
R4573 T5110 T5109 prep at,sec
R4574 T5111 T5112 nummod 72,°
R4575 T5112 T5114 nummod °,cDNA
R4576 T5113 T5114 compound C.,cDNA
R4577 T5114 T5110 pobj cDNA,at
R4578 T5115 T5109 prep for,sec
R4579 T5116 T5115 pobj β-actin,for
R4580 T5117 T5118 auxpass was,amplified
R4581 T5118 T5092 conj amplified,consisted
R4582 T5119 T5118 prep for,amplified
R4583 T5120 T5121 nummod 28,cycles
R4584 T5121 T5119 pobj cycles,for
R4585 T5122 T5118 advcl using,amplified
R4586 T5123 T5124 det the,oligos
R4587 T5124 T5122 dobj oligos,using
R4588 T5125 T5124 punct :,oligos
R4589 T5126 T5124 appos sense,oligos
R4590 T5127 T5131 nummod 5,′
R4591 T5128 T5130 punct ′,GGCATCGTGATGGACTCCG-3
R4592 T5129 T5130 punct -,GGCATCGTGATGGACTCCG-3
R4593 T5130 T5131 compound GGCATCGTGATGGACTCCG-3,′
R4594 T5131 T5124 appos ′,oligos
R4595 T5132 T5131 cc and,′
R4596 T5133 T5131 conj antisense,′
R4597 T5134 T5133 nummod 5,antisense
R4598 T5135 T5137 nummod ′,′
R4599 T5136 T5137 compound GCTGGAAGGTGGACAGCGA-3,′
R4600 T5137 T5133 appos ′,antisense
R4601 T5138 T5092 punct .,consisted
R4602 T5139 T5143 nsubjpass cDNA,amplified
R4603 T5140 T5139 prep for,cDNA
R4604 T5141 T5140 pobj TNF-α,for
R4605 T5142 T5143 auxpass was,amplified
R4606 T5143 T5143 ROOT amplified,amplified
R4607 T5144 T5143 prep for,amplified
R4608 T5145 T5146 nummod 35,cycles
R4609 T5146 T5144 pobj cycles,for
R4610 T5147 T5143 advcl using,amplified
R4611 T5148 T5149 det the,oligos
R4612 T5149 T5147 dobj oligos,using
R4613 T5150 T5149 punct :,oligos
R4614 T5151 T5149 appos sense,oligos
R4615 T5152 T5155 nummod 5,AAGCCTGTAGCCCATGTTGT-3
R4616 T5153 T5155 punct ′,AAGCCTGTAGCCCATGTTGT-3
R4617 T5154 T5155 punct -,AAGCCTGTAGCCCATGTTGT-3
R4618 T5155 T5156 compound AAGCCTGTAGCCCATGTTGT-3,′
R4619 T5156 T5149 appos ′,oligos
R4620 T5157 T5156 cc and,′
R4621 T5158 T5163 nmod antisense,′
R4622 T5159 T5158 nummod 5,antisense
R4623 T5160 T5162 punct ′,CAGATAGATGGGCTCATACC-3
R4624 T5161 T5162 punct -,CAGATAGATGGGCTCATACC-3
R4637 T5174 T5176 det the,sense
R4638 T5175 T5176 nsubj oligos,sense
R4639 T5176 T5173 dobj sense,using
R4640 T5177 T5181 nummod 5,′
R4641 T5178 T5180 punct ′,GTAGCAGCCGCAGTCATAATGG-3
R4642 T5179 T5180 punct -,GTAGCAGCCGCAGTCATAATGG-3
R4643 T5180 T5181 compound GTAGCAGCCGCAGTCATAATGG-3,′
R4644 T5181 T5176 appos ′,sense
R4645 T5182 T5181 cc and,′
R4646 T5183 T5181 conj antisense,′
R4647 T5184 T5183 nummod 5,antisense
R4648 T5185 T5189 punct ′,′
R4649 T5186 T5189 punct -,′
R4650 T5187 T5189 det A,′
R4651 T5188 T5189 amod TGCTGTTGTATCTGACTGAGG-3,′
R4652 T5189 T5181 appos ′,′
R4653 T5190 T5169 punct .,amplified
R4748 T5335 T5337 compound NF-kB,Reporter
R4749 T5336 T5337 compound Transcription,Reporter
R4750 T5337 T5363 nsubj Reporter,contains
R4751 T5338 T5339 compound Gene,Assay
R4752 T5339 T5340 compound Assay,The
R4753 T5340 T5342 det The,3XMHC-luc
R4754 T5341 T5342 compound plasmid,3XMHC-luc
R4755 T5342 T5363 nsubj 3XMHC-luc,contains
R4756 T5343 T5346 punct (,gift
R4757 T5344 T5346 det a,gift
R4758 T5345 T5346 amod generous,gift
R4759 T5346 T5342 appos gift,3XMHC-luc
R4760 T5347 T5346 prep from,gift
R4761 T5348 T5350 compound Drs.,Westwick
R4762 T5349 T5350 compound J.,Westwick
R4763 T5350 T5347 pobj Westwick,from
R4764 T5351 T5350 cc and,Westwick
R4765 T5352 T5353 compound D.A.,Brenner
R4766 T5353 T5350 conj Brenner,Westwick
R4767 T5354 T5353 punct ",",Brenner
R4768 T5355 T5353 appos University,Brenner
R4769 T5356 T5355 prep of,University
R4770 T5357 T5358 compound North,Carolina
R4771 T5358 T5356 pobj Carolina,of
R4772 T5359 T5353 punct ",",Brenner
R4773 T5360 T5361 compound Chapel,Hill
R4774 T5361 T5353 appos Hill,Brenner
R4775 T5362 T5353 punct ),Brenner
R4776 T5363 T5363 ROOT contains,contains
R4777 T5364 T5365 nummod three,copies
R4778 T5365 T5363 dobj copies,contains
R4779 T5366 T5365 prep of,copies
R4780 T5367 T5368 amod NF-κB-responsive,element
R4781 T5368 T5366 pobj element,of
R4782 T5369 T5368 prep from,element
R4783 T5370 T5372 det the,class
R4784 T5371 T5372 compound MHC,class
R4785 T5372 T5369 pobj class,from
R4786 T5373 T5374 compound I,locus
R4787 T5374 T5366 pobj locus,of
R4788 T5375 T5365 punct ",",copies
R4789 T5376 T5365 acl placed,copies
R4790 T5377 T5376 advmod upstream,placed
R4791 T5378 T5377 prep of,upstream
R4792 T5379 T5381 det the,gene
R4793 T5380 T5381 compound luciferase,gene
R4794 T5381 T5378 pobj gene,of
R4795 T5382 T5363 punct .,contains
R4796 T5383 T5386 amod Human,cells
R4797 T5384 T5386 amod monocytic,cells
R4798 T5385 T5386 compound THP-1,cells
R4799 T5386 T5389 nsubjpass cells,transfected
R4800 T5387 T5389 auxpass were,transfected
R4801 T5388 T5389 advmod transiently,transfected
R4802 T5389 T5389 ROOT transfected,transfected
R4803 T5390 T5392 mark as,described
R4804 T5391 T5392 advmod previously,described
R4805 T5392 T5389 advcl described,transfected
R4806 T5393 T5395 compound [,]
R4807 T5394 T5395 compound 30,]
R4808 T5395 T5392 dobj ],described
R4809 T5396 T5389 punct ",",transfected
R4810 T5397 T5389 cc and,transfected
R4811 T5398 T5399 advmod then,cultured
R4812 T5399 T5389 conj cultured,transfected
R4813 T5400 T5399 prep for,cultured
R4814 T5401 T5402 nummod 4,h
R4815 T5402 T5400 pobj h,for
R4816 T5403 T5402 advmod alone,h
R4817 T5404 T5400 cc or,for
R4818 T5405 T5400 conj with,for
R4819 T5406 T5407 amod increasing,concentration
R4820 T5407 T5405 pobj concentration,with
R4821 T5408 T5407 prep of,concentration
R4822 T5409 T5410 det either,C10
R4823 T5410 T5408 pobj C10,of
R4824 T5411 T5410 cc or,C10
R4825 T5412 T5410 conj C40,C10
R4826 T5413 T5389 punct .,transfected
R4827 T5414 T5415 amod Luciferase,activity
R4828 T5415 T5417 nsubjpass activity,determined
R4829 T5416 T5417 auxpass was,determined
R4830 T5417 T5417 ROOT determined,determined
R4831 T5418 T5417 advcl using,determined
R4832 T5419 T5420 det a,luminometer
R4833 T5420 T5418 dobj luminometer,using
R4834 T5421 T5420 punct (,luminometer
R4835 T5422 T5420 appos Monolight,luminometer
R4836 T5423 T5424 nummod 2010,Luminometer
R4837 T5424 T5424 ROOT Luminometer,Luminometer
R4838 T5425 T5424 punct ",",Luminometer
R4839 T5426 T5427 compound Ann,Arbor
R4840 T5427 T5424 conj Arbor,Luminometer
R4841 T5428 T5427 punct ",",Arbor
R4842 T5429 T5427 conj MI,Arbor
R4843 T5430 T5422 punct ),Monolight
R4844 T5431 T5417 punct .,determined
R5294 T5933 T5937 amod Western,cells
R5295 T5934 T5936 compound Blot,THP-1
R5296 T5935 T5936 compound Analysis,THP-1
R5297 T5936 T5937 compound THP-1,cells
R5298 T5937 T5939 nsubjpass cells,stimulated
R5299 T5938 T5939 auxpass were,stimulated
R5300 T5939 T5939 ROOT stimulated,stimulated
R5301 T5940 T5939 prep for,stimulated
R5302 T5941 T5942 amod various,lengths
R5303 T5942 T5940 pobj lengths,for
R5304 T5943 T5942 prep of,lengths
R5305 T5944 T5943 pobj time,of
R5306 T5945 T5942 prep with,lengths
R5307 T5946 T5947 nummod 0.1,mg/ml
R5308 T5947 T5945 pobj mg/ml,with
R5309 T5948 T5947 nummod C10,mg/ml
R5310 T5949 T5948 cc or,C10
R5311 T5950 T5948 conj C40,C10
R5312 T5951 T5950 punct ",",C40
R5313 T5952 T5950 cc or,C40
R5314 T5953 T5954 nummod 10,µg
R5315 T5954 T5957 compound µg,LPS
R5316 T5955 T5957 compound /,LPS
R5317 T5956 T5957 compound ml,LPS
R5318 T5957 T5950 conj LPS,C40
R5319 T5958 T5939 punct .,stimulated
R5320 T5959 T5962 nsubjpass Cells,pelleted
R5321 T5960 T5962 auxpass were,pelleted
R5322 T5961 T5962 advmod then,pelleted
R5323 T5962 T5962 ROOT pelleted,pelleted
R5324 T5963 T5962 punct ",",pelleted
R5325 T5964 T5962 conj washed,pelleted
R5326 T5965 T5964 cc and,washed
R5327 T5966 T5964 conj homogenised,washed
R5328 T5967 T5966 prep in,homogenised
R5329 T5968 T5969 compound lysis,buffer
R5330 T5969 T5967 pobj buffer,in
R5331 T5970 T5973 punct (,Hepes
R5332 T5971 T5973 nummod 10,Hepes
R5333 T5972 T5973 compound mM,Hepes
R5334 T5973 T5966 appos Hepes,homogenised
R5335 T5974 T5973 punct ",",Hepes
R5336 T5975 T5973 conj pH,Hepes
R5337 T5976 T5975 nummod 7.9,pH
R5338 T5977 T5975 punct ",",pH
R5339 T5978 T5980 nummod 150,NaCl
R5340 T5979 T5980 compound mM,NaCl
R5341 T5980 T5975 conj NaCl,pH
R5342 T5981 T5980 punct ",",NaCl
R5343 T5982 T5984 nummod 1,EDTA
R5344 T5983 T5984 compound mM,EDTA
R5345 T5984 T5980 conj EDTA,NaCl
R5346 T5985 T5984 punct ",",EDTA
R5347 T5986 T5987 nummod 0.6,%
R5348 T5987 T5988 compound %,NP-40
R5349 T5988 T5984 conj NP-40,EDTA
R5350 T5989 T5988 punct ",",NP-40
R5351 T5990 T5988 cc and,NP-40
R5352 T5991 T5993 nummod 0.5,PMSF
R5353 T5992 T5993 compound mM,PMSF
R5354 T5993 T5988 conj PMSF,NP-40
R5355 T5994 T5993 punct ),PMSF
R5356 T5995 T5988 prep on,NP-40
R5357 T5996 T5995 pobj ice,on
R5358 T5997 T5962 punct .,pelleted
R5359 T5998 T6000 nsubjpass Homogenates,sonicated
R5360 T5999 T6000 auxpass were,sonicated
R5361 T6000 T6000 ROOT sonicated,sonicated
R5362 T6001 T6000 punct ",",sonicated
R5363 T6002 T6000 conj centrifuged,sonicated
R5364 T6003 T6002 prep at,centrifuged
R5365 T6004 T6005 nummod "10,000",rpm
R5366 T6005 T6003 pobj rpm,at
R5367 T6006 T6007 aux to,remove
R5368 T6007 T6002 advcl remove,centrifuged
R5369 T6008 T6009 amod cellular,debris
R5370 T6009 T6007 dobj debris,remove
R5371 T6010 T6007 punct ",",remove
R5372 T6011 T6007 cc and,remove
R5373 T6012 T6013 amod supernatant,collected
R5374 T6013 T6002 conj collected,centrifuged
R5375 T6014 T6000 punct .,sonicated
R5376 T6015 T6016 compound Protein,concentration
R5377 T6016 T6018 nsubjpass concentration,determined
R5378 T6017 T6018 auxpass was,determined
R5379 T6018 T6018 ROOT determined,determined
R5380 T6019 T6018 advcl using,determined
R5381 T6020 T6023 det the,Assay
R5382 T6021 T6023 compound DC,Assay
R5383 T6022 T6023 compound Protein,Assay
R5384 T6023 T6019 dobj Assay,using
R5385 T6024 T6023 punct (,Assay
R5386 T6025 T6023 appos Bio-Rad,Assay
R5387 T6026 T6023 punct ),Assay
R5388 T6027 T6018 punct .,determined
R5389 T6028 T6038 nsubjpass Proteins,resolved
R5390 T6029 T6028 prep in,Proteins
R5391 T6030 T6029 pobj samples,in
R5392 T6031 T6030 punct (,samples
R5393 T6032 T6033 nummod 15,µg
R5394 T6033 T6038 nsubjpass µg,resolved
R5395 T6034 T6035 amod total,proteins
R5396 T6035 T6033 appos proteins,µg
R5397 T6036 T6033 punct ),µg
R5398 T6037 T6038 auxpass were,resolved
R5399 T6038 T6038 ROOT resolved,resolved
R5400 T6039 T6038 prep in,resolved
R5401 T6040 T6045 det a,gel
R5402 T6041 T6045 amod denaturing,gel
R5403 T6042 T6043 nummod 12,%
R5404 T6043 T6045 compound %,gel
R5405 T6044 T6045 compound polyacrylamide,gel
R5406 T6045 T6039 pobj gel,in
R5407 T6046 T6038 cc and,resolved
R5408 T6047 T6038 conj transferred,resolved
R5409 T6048 T6047 prep to,transferred
R5410 T6049 T6051 det a,membrane
R5411 T6050 T6051 amod nitrocellulose,membrane
R5412 T6051 T6048 pobj membrane,to
R5413 T6052 T6038 punct .,resolved
R5414 T6053 T6054 amod I-κBα,protein
R5415 T6054 T6056 nsubjpass protein,detected
R5416 T6055 T6056 auxpass was,detected
R5417 T6056 T6056 ROOT detected,detected
R5418 T6057 T6056 advcl using,detected
R5419 T6058 T6061 det a,antibody
R5420 T6059 T6061 nmod rabbit,antibody
R5421 T6060 T6061 compound polyclonal,antibody
R5422 T6061 T6057 dobj antibody,using
R5423 T6062 T6061 punct (,antibody
R5424 T6063 T6064 compound Santa,Cruz
R5425 T6064 T6065 compound Cruz,Biotechnology
R5426 T6065 T6061 appos Biotechnology,antibody
R5427 T6066 T6065 punct ",",Biotechnology
R5428 T6067 T6065 appos CA,Biotechnology
R5429 T6068 T6061 punct ),antibody
R5430 T6069 T6056 advcl followed,detected
R5431 T6070 T6069 agent by,followed
R5432 T6071 T6076 det a,antibody
R5433 T6072 T6076 amod horseradish,antibody
R5434 T6073 T6076 amod peroxidase-coupled,antibody
R5435 T6074 T6076 compound goat,antibody
R5436 T6075 T6076 compound polyclonal,antibody
R5437 T6076 T6070 pobj antibody,by
R5438 T6077 T6076 prep against,antibody
R5439 T6078 T6079 compound rabbit,Ig
R5440 T6079 T6077 pobj Ig,against
R5441 T6080 T6082 punct (,Laboratories
R5442 T6081 T6082 compound Caltag,Laboratories
R5443 T6082 T6079 appos Laboratories,Ig
R5444 T6083 T6082 punct ),Laboratories
R5445 T6084 T6056 punct .,detected
R5446 T6085 T6090 advmod Finally,revealed
R5447 T6086 T6090 punct ",",revealed
R5448 T6087 T6088 compound IκB,bands
R5449 T6088 T6090 nsubjpass bands,revealed
R5450 T6089 T6090 auxpass were,revealed
R5451 T6090 T6090 ROOT revealed,revealed
R5452 T6091 T6090 advcl using,revealed
R5453 T6092 T6096 det the,system
R5454 T6093 T6096 nmod ECL,system
R5455 T6094 T6096 compound ™,system
R5456 T6095 T6096 compound detection,system
R5457 T6096 T6091 dobj system,using
R5458 T6097 T6096 punct (,system
R5459 T6098 T6100 compound Amersham,Biotech
R5460 T6099 T6100 compound Pharmacia,Biotech
R5461 T6100 T6096 appos Biotech,system
R5462 T6101 T6096 punct ",",system
R5463 T6102 T6103 compound Les,Ullis
R5464 T6103 T6096 appos Ullis,system
R5465 T6104 T6103 punct ",",Ullis
R5466 T6105 T6103 appos France,Ullis
R5467 T6106 T6096 punct ),system
R5468 T6107 T6090 prep according,revealed
R5469 T6108 T6107 prep to,according
R5470 T6109 T6110 det the,manufacturers
R5471 T6110 T6112 poss manufacturers,instruction
R5472 T6111 T6110 case ',manufacturers
R5473 T6112 T6108 pobj instruction,to
R5474 T6113 T6090 punct .,revealed
R5475 T6114 T6121 nsubj Antibody,was
R5476 T6115 T6114 prep to,Antibody
R5477 T6116 T6115 pobj α-Tubulin,to
R5478 T6117 T6119 punct (,Cruz
R5479 T6118 T6119 compound Santa,Cruz
R5480 T6119 T6116 appos Cruz,α-Tubulin
R5481 T6120 T6116 punct ),α-Tubulin
R5482 T6121 T6121 ROOT was,was
R5483 T6122 T6121 attr use,was
R5484 T6123 T6122 prep as,use
R5485 T6124 T6123 pcomp loading,as
R5486 T6125 T6124 dobj control,loading
R5487 T6126 T6121 punct .,was
R5488 T6127 T6134 prep For,stimulated
R5489 T6128 T6129 amod nuclear,NF-κB
R5490 T6129 T6127 pobj NF-κB,For
R5491 T6130 T6134 punct ",",stimulated
R5492 T6131 T6132 compound THP-1,cells
R5493 T6132 T6134 nsubjpass cells,stimulated
R5494 T6133 T6134 auxpass were,stimulated
R5495 T6134 T6151 ccomp stimulated,pelleted
R5496 T6135 T6134 prep with,stimulated
R5497 T6136 T6137 nummod 1,mg/ml
R5498 T6137 T6135 pobj mg/ml,with
R5499 T6138 T6137 nummod C10,mg/ml
R5500 T6139 T6138 cc or,C10
R5501 T6140 T6138 conj C40,C10
R5502 T6141 T6140 prep for,C40
R5503 T6142 T6143 nummod 30,minutes
R5504 T6143 T6141 pobj minutes,for
R5505 T6144 T6143 prep at,minutes
R5506 T6145 T6146 nummod 37,°
R5507 T6146 T6148 compound °,Cells
R5508 T6147 T6148 compound C.,Cells
R5509 T6148 T6144 pobj Cells,at
R5510 T6149 T6151 auxpass were,pelleted
R5511 T6150 T6151 advmod then,pelleted
R5512 T6151 T6151 ROOT pelleted,pelleted
R5513 T6152 T6151 cc and,pelleted
R5514 T6153 T6151 conj nuclei,pelleted
R5515 T6154 T6151 conj separated,pelleted
R5516 T6155 T6156 mark as,described
R5517 T6156 T6151 advcl described,pelleted
R5518 T6157 T6159 nmod [,]
R5519 T6158 T6159 nummod 31,]
R5520 T6159 T6156 dobj ],described
R5521 T6160 T6151 punct .,pelleted
R5522 T6161 T6163 nsubjpass Nuclei,washed
R5523 T6162 T6163 auxpass were,washed
R5524 T6163 T6163 ROOT washed,washed
R5525 T6164 T6163 cc and,washed
R5526 T6165 T6163 conj homogenized,washed
R5527 T6166 T6165 advmod directly,homogenized
R5528 T6167 T6165 prep in,homogenized
R5529 T6168 T6167 pcomp loading,in
R5530 T6169 T6172 punct (,buffer
R5531 T6170 T6172 nmod Laemli,buffer
R5532 T6171 T6172 punct ),buffer
R5533 T6172 T6168 dobj buffer,loading
R5534 T6173 T6172 cc and,buffer
R5535 T6174 T6163 conj heated,washed
R5536 T6175 T6174 prep for,heated
R5537 T6176 T6177 nummod 5,minutes
R5538 T6177 T6175 pobj minutes,for
R5539 T6178 T6174 prep at,heated
R5540 T6179 T6182 nummod 100,Proteins
R5541 T6180 T6182 nummod °,Proteins
R5542 T6181 T6182 compound C.,Proteins
R5543 T6182 T6178 pobj Proteins,at
R5544 T6183 T6182 prep in,Proteins
R5545 T6184 T6183 pobj samples,in
R5546 T6185 T6186 auxpass were,resolved
R5547 T6186 T6163 conj resolved,washed
R5548 T6187 T6186 prep in,resolved
R5549 T6188 T6193 det a,gel
R5550 T6189 T6193 amod denaturing,gel
R5551 T6190 T6191 nummod 8,%
R5552 T6191 T6193 compound %,gel
R5553 T6192 T6193 compound polyacrylamide,gel
R5554 T6193 T6187 pobj gel,in
R5555 T6194 T6186 cc and,resolved
R5556 T6195 T6186 conj transferred,resolved
R5557 T6196 T6195 prep to,transferred
R5558 T6197 T6199 det a,fluoride
R5559 T6198 T6199 compound polyvinylidine,fluoride
R5560 T6199 T6196 pobj fluoride,to
R5561 T6200 T6199 punct (,fluoride
R5562 T6201 T6199 appos PVDF,fluoride
R5563 T6202 T6199 punct ),fluoride
R5564 T6203 T6195 dobj membrane,transferred
R5565 T6204 T6205 punct (,Immobilon-P
R5566 T6205 T6203 appos Immobilon-P,membrane
R5567 T6206 T6163 punct ;,washed
R5568 T6207 T6207 ROOT Millipore,Millipore
R5569 T6208 T6207 punct ",",Millipore
R5570 T6209 T6207 conj Bedford,Millipore
R5571 T6210 T6209 punct ",",Bedford
R5572 T6211 T6209 conj MA,Bedford
R5573 T6212 T6209 punct ),Bedford
R5574 T6213 T6207 punct .,Millipore
R5575 T6214 T6216 nsubjpass Membranes,incubated
R5576 T6215 T6216 auxpass were,incubated
R5577 T6216 T6216 ROOT incubated,incubated
R5578 T6217 T6216 prep in,incubated
R5579 T6218 T6217 pcomp blocking,in
R5580 T6219 T6218 dobj buffer,blocking
R5581 T6220 T6223 punct (,BSA
R5582 T6221 T6222 nummod 1,%
R5583 T6222 T6223 npadvmod %,BSA
R5584 T6223 T6218 dobj BSA,blocking
R5585 T6224 T6218 punct ",",blocking
R5586 T6225 T6224 oprd in,","
R5587 T6226 T6225 pobj PBS,in
R5588 T6227 T6225 punct ),in
R5589 T6228 T6216 prep for,incubated
R5590 T6229 T6230 nummod two,hours
R5591 T6230 T6228 pobj hours,for
R5592 T6231 T6230 prep at,hours
R5593 T6232 T6233 compound room,temperature
R5594 T6233 T6231 pobj temperature,at
R5595 T6234 T6216 punct .,incubated
R5596 T6235 T6238 nsubjpass Membranes,probed
R5597 T6236 T6238 auxpass were,probed
R5598 T6237 T6238 advmod subsequently,probed
R5599 T6238 T6238 ROOT probed,probed
R5600 T6239 T6238 prep with,probed
R5601 T6240 T6242 det the,antibody
R5602 T6241 T6242 amod corresponding,antibody
R5603 T6242 T6239 pobj antibody,with
R5604 T6243 T6238 prep in,probed
R5605 T6244 T6243 pcomp blocking,in
R5606 T6245 T6244 dobj buffer,blocking
R5607 T6246 T6247 punct ",",overnight
R5608 T6247 T6244 npadvmod overnight,blocking
R5609 T6248 T6238 punct .,probed
R5610 T6249 T6254 nmod Rabbit,subunit
R5611 T6250 T6254 amod polyclonal,subunit
R5612 T6251 T6254 nmod antibody,subunit
R5613 T6252 T6254 nmod anti-NF-κB,subunit
R5614 T6253 T6254 amod p50,subunit
R5615 T6254 T6272 nsubjpass subunit,used
R5616 T6255 T6254 punct (,subunit
R5617 T6256 T6257 nmod #,sc-114
R5618 T6257 T6272 nsubjpass sc-114,used
R5619 T6258 T6257 punct ),sc-114
R5620 T6259 T6257 cc or,sc-114
R5621 T6260 T6261 amod anti-NF-κB,p65
R5622 T6261 T6257 conj p65,sc-114
R5623 T6262 T6261 acl subunit,p65
R5624 T6263 T6261 punct (,p65
R5625 T6264 T6265 nmod #,sc-109
R5626 T6265 T6261 appos sc-109,p65
R5627 T6266 T6261 punct ),p65
R5628 T6267 T6261 prep from,p65
R5629 T6268 T6269 compound Santa,Cruz
R5630 T6269 T6270 compound Cruz,Biotechnology
R5631 T6270 T6267 pobj Biotechnology,from
R5632 T6271 T6272 auxpass were,used
R5633 T6272 T6272 ROOT used,used
R5634 T6273 T6272 punct .,used
R5635 T6274 T6276 nsubjpass Membranes,washed
R5636 T6275 T6276 auxpass were,washed
R5637 T6276 T6276 ROOT washed,washed
R5638 T6277 T6278 nummod six,times
R5639 T6278 T6276 npadvmod times,washed
R5640 T6279 T6276 prep in,washed
R5641 T6280 T6279 pobj PBS,in
R5642 T6281 T6276 prep with,washed
R5643 T6282 T6283 nummod 0.05,%
R5644 T6283 T6288 nummod %,minutes
R5645 T6284 T6288 nummod Tween,minutes
R5646 T6285 T6288 nummod 20,minutes
R5647 T6286 T6288 punct ",",minutes
R5648 T6287 T6288 nummod 5,minutes
R5649 T6288 T6281 pobj minutes,with
R5650 T6289 T6290 det each,time
R5651 T6290 T6276 npadvmod time,washed
R5652 T6291 T6276 punct ",",washed
R5653 T6292 T6276 cc and,washed
R5654 T6293 T6276 conj incubated,washed
R5655 T6294 T6293 prep with,incubated
R5656 T6295 T6297 det a,dilution
R5657 T6296 T6297 nummod 1/3000,dilution
R5658 T6297 T6294 pobj dilution,with
R5659 T6298 T6297 prep of,dilution
R5660 T6299 T6300 amod HRP-conjugated,F
R5661 T6300 T6298 pobj F,of
R5662 T6301 T6308 punct (,IgG
R5663 T6302 T6306 nmod ab,goat
R5664 T6303 T6302 case ',ab
R5665 T6304 T6302 punct ),ab
R5666 T6305 T6302 nummod 2,ab
R5667 T6306 T6308 nmod goat,IgG
R5668 T6307 T6308 compound anti-rabbit,IgG
R5669 T6308 T6314 nmod IgG,milk
R5670 T6309 T6308 prep in,IgG
R5671 T6310 T6311 nummod 5,%
R5672 T6311 T6314 nmod %,milk
R5673 T6312 T6314 amod nonfat,milk
R5674 T6313 T6314 amod dry,milk
R5675 T6314 T6297 appos milk,dilution
R5676 T6315 T6314 cc and,milk
R5677 T6316 T6317 nummod 0.05,%
R5678 T6317 T6314 conj %,milk
R5679 T6318 T6319 nummod Tween,20
R5680 T6319 T6322 dep 20,for
R5681 T6320 T6319 prep in,20
R5682 T6321 T6320 pobj PBS,in
R5683 T6322 T6293 conj for,incubated
R5684 T6323 T6324 nummod 1,hour
R5685 T6324 T6322 pobj hour,for
R5686 T6325 T6322 prep at,for
R5687 T6326 T6327 compound room,temperature
R5688 T6327 T6325 pobj temperature,at
R5689 T6328 T6276 punct .,washed
R5690 T6329 T6345 prep After,detected
R5691 T6330 T6329 pcomp washing,After
R5692 T6331 T6333 nummod six,times
R5693 T6332 T6331 amod more,six
R5694 T6333 T6330 dobj times,washing
R5695 T6334 T6330 prep in,washing
R5696 T6335 T6334 pobj PBS,in
R5697 T6336 T6330 prep with,washing
R5698 T6337 T6338 nummod 0.05,%
R5699 T6338 T6345 nsubj %,detected
R5700 T6339 T6340 nummod Tween,20
R5701 T6340 T6345 dep 20,detected
R5702 T6341 T6345 punct ",",detected
R5703 T6342 T6343 amod antibody-reactive,proteins
R5704 T6343 T6345 nsubjpass proteins,detected
R5705 T6344 T6345 auxpass were,detected
R5706 T6345 T6345 ROOT detected,detected
R5707 T6346 T6345 advcl using,detected
R5708 T6347 T6349 det a,substrate
R5709 T6348 T6349 compound chemiluminescence,substrate
R5710 T6349 T6346 dobj substrate,using
R5711 T6350 T6359 punct (,according
R5712 T6351 T6359 dep SuperSignal,according
R5713 T6352 T6351 punct ;,SuperSignal
R5714 T6353 T6351 conj Pierce,SuperSignal
R5715 T6354 T6353 punct ",",Pierce
R5716 T6355 T6353 conj Rockford,Pierce
R5717 T6356 T6355 punct ",",Rockford
R5718 T6357 T6355 conj IL,Rockford
R5719 T6358 T6359 punct ),according
R5720 T6359 T6345 prep according,detected
R5721 T6360 T6359 prep to,according
R5722 T6361 T6362 det the,manufacturer
R5723 T6362 T6364 poss manufacturer,instructions
R5724 T6363 T6362 case 's,manufacturer
R5725 T6364 T6360 pobj instructions,to
R5726 T6365 T6345 punct .,detected
R5727 T6366 T6367 aux To,confirm
R5728 T6367 T6380 advcl confirm,were
R5729 T6368 T6374 mark that,loaded
R5730 T6369 T6370 amod equivalent,amounts
R5731 T6370 T6374 nsubjpass amounts,loaded
R5732 T6371 T6370 prep of,amounts
R5733 T6372 T6371 pobj protein,of
R5734 T6373 T6374 auxpass were,loaded
R5735 T6374 T6367 ccomp loaded,confirm
R5736 T6375 T6374 prep in,loaded
R5737 T6376 T6377 det each,line
R5738 T6377 T6375 pobj line,in
R5739 T6378 T6380 punct ",",were
R5740 T6379 T6380 nsubj membranes,were
R5741 T6380 T6380 ROOT were,were
R5742 T6381 T6380 advmod also,were
R5743 T6382 T6383 amod Western,blotted
R5744 T6383 T6380 attr blotted,were
R5745 T6384 T6383 prep for,blotted
R5746 T6385 T6384 pobj ERK,for
R5747 T6386 T6387 mark as,described
R5748 T6387 T6380 advcl described,were
R5749 T6388 T6390 nmod [,]
R5750 T6389 T6390 nummod 32,]
R5751 T6390 T6387 dobj ],described
R5752 T6391 T6380 punct .,were
R5773 T6486 T6503 nsubjpass Analysis,performed
R5774 T6487 T6486 prep of,Analysis
R5775 T6488 T6489 compound NF-κB,Activation
R5776 T6489 T6487 pobj Activation,of
R5777 T6490 T6489 prep by,Activation
R5778 T6491 T6494 compound Flow,activation
R5779 T6492 T6494 compound Cytometry,activation
R5780 T6493 T6494 compound Nuclear,activation
R5781 T6494 T6490 pobj activation,by
R5782 T6495 T6494 prep of,activation
R5783 T6496 T6498 compound NF,κΒ
R5784 T6497 T6498 compound −,κΒ
R5785 T6498 T6495 pobj κΒ,of
R5786 T6499 T6486 prep by,Analysis
R5787 T6500 T6501 compound flow,cytometry
R5788 T6501 T6499 pobj cytometry,by
R5789 T6502 T6503 auxpass was,performed
R5790 T6503 T6503 ROOT performed,performed
R5791 T6504 T6505 mark as,described
R5792 T6505 T6503 advcl described,performed
R5793 T6506 T6508 nmod [,]
R5794 T6507 T6508 nummod 31,]
R5795 T6508 T6505 dobj ],described
R5796 T6509 T6503 punct .,performed
R5837 T6558 T6559 compound Statistical,Analysis
R5838 T6559 T6563 nsubjpass Analysis,expressed
R5839 T6560 T6561 det The,results
R5840 T6561 T6563 nsubjpass results,expressed
R5841 T6562 T6563 auxpass were,expressed
R5842 T6563 T6563 ROOT expressed,expressed
R5843 T6564 T6563 prep as,expressed
R5844 T6565 T6569 det the,S.E.M.
R5845 T6566 T6569 amod mean,S.E.M.
R5846 T6567 T6568 compound value,±
R5847 T6568 T6569 compound ±,S.E.M.
R5848 T6569 T6564 pobj S.E.M.,as
R5849 T6570 T6569 prep of,S.E.M.
R5850 T6571 T6572 amod individual,experiments
R5851 T6572 T6570 pobj experiments,of
R5852 T6573 T6563 punct .,expressed
R5853 T6574 T6576 det The,significance
R5854 T6575 T6576 amod statistical,significance
R5855 T6576 T6584 nsubjpass significance,assessed
R5856 T6577 T6576 prep of,significance
R5857 T6578 T6579 det the,differences
R5858 T6579 T6577 pobj differences,of
R5859 T6580 T6579 prep between,differences
R5860 T6581 T6582 amod mean,values
R5861 T6582 T6580 pobj values,between
R5862 T6583 T6584 auxpass was,assessed
R5863 T6584 T6584 ROOT assessed,assessed
R5864 T6585 T6584 agent by,assessed
R5865 T6586 T6587 det the,Student
R5866 T6587 T6591 poss Student,analysis
R5867 T6588 T6587 case 's,Student
R5868 T6589 T6591 amod t-test,analysis
R5869 T6590 T6589 cc and,t-test
R5870 T6591 T6585 pobj analysis,by
R5871 T6592 T6591 prep of,analysis
R5872 T6593 T6592 pobj variance,of
R5873 T6594 T6595 punct (,ANOVA
R5874 T6595 T6593 appos ANOVA,variance
R5875 T6596 T6584 punct ),assessed
R5876 T6597 T6584 punct .,assessed
R2153 T2437 T2421 conj medium,preparations
R2154 T2438 T2437 punct ",",medium
R2155 T2439 T2437 punct (,medium
R2156 T2440 T2437 appos ∼,medium
R2157 T2441 T2442 nummod 40,kDa
R2158 T2442 T2440 appos kDa,∼
R2159 T2443 T2437 punct ;,medium
R2160 T2444 T2421 appos C40,preparations
R2161 T2445 T2421 punct ),preparations
R2162 T2446 T2447 amod molecular,weight
R2163 T2447 T2416 conj weight,Preparation
R2164 T2448 T2449 auxpass were,prepared
R2165 T2449 T2449 ROOT prepared,prepared
R2166 T2450 T2449 prep from,prepared
R2167 T2451 T2452 amod native,iota-carrageenan
R2168 T2452 T2450 pobj iota-carrageenan,from
R2169 T2453 T2452 acl extracted,iota-carrageenan
R2170 T2454 T2453 prep from,extracted
R2171 T2455 T2456 compound Euchema,spinosum
R2172 T2456 T2454 pobj spinosum,from
R2173 T2457 T2459 punct (,provided
R2174 T2458 T2459 advmod generously,provided
R2175 T2459 T2452 acl provided,iota-carrageenan
R2176 T2460 T2459 agent by,provided
R2177 T2461 T2463 compound Sanofi,Industry
R2178 T2462 T2463 compound Biosystems,Industry
R2179 T2463 T2460 pobj Industry,by
R2205 T2489 T2487 pobj °,to
R2206 T2490 T2491 compound C.,Then
R2207 T2491 T2497 advmod Then,submitted
R2208 T2492 T2497 punct ",",submitted
R2209 T2493 T2495 det the,solution
R2210 T2494 T2495 compound carrageenan,solution
R2211 T2495 T2497 nsubjpass solution,submitted
R2212 T2496 T2497 auxpass was,submitted
R2213 T2497 T2497 ROOT submitted,submitted
R2214 T2498 T2497 prep to,submitted
R2215 T2499 T2501 nummod two,treatments
R2216 T2500 T2501 amod different,treatments
R2217 T2501 T2498 pobj treatments,to
R2218 T2502 T2503 aux to,obtain
R2219 T2503 T2497 xcomp obtain,submitted
R2220 T2504 T2505 advmod both,low
R2221 T2505 T2510 amod low,fractions
R2222 T2506 T2505 cc and,low
R2223 T2507 T2505 conj medium,low
R2224 T2508 T2509 amod molecular,weight
R2225 T2509 T2510 compound weight,fractions
R2226 T2510 T2503 dobj fractions,obtain
R2227 T2511 T2497 punct .,submitted
R2228 T2512 T2524 advmod Briefly,hydrolyzed
R2229 T2513 T2524 punct ",",hydrolyzed
R2230 T2514 T2524 prep for,hydrolyzed
R2231 T2515 T2519 det the,fraction
R2493 T2777 T2779 det the,fractions
R2494 T2778 T2779 nummod two,fractions
R2495 T2779 T2781 nsubjpass fractions,dissolved
R2496 T2780 T2781 auxpass were,dissolved
R2497 T2781 T2781 ROOT dissolved,dissolved
R2498 T2782 T2781 prep in,dissolved
R2499 T2783 T2784 amod complete,medium
R2500 T2784 T2782 pobj medium,in
R2501 T2785 T2781 prep during,dissolved
R2502 T2786 T2787 nummod 30,min
R2503 T2787 T2785 pobj min,during
R2504 T2788 T2787 prep at,min
R2505 T2789 T2790 nummod 56,°
R2506 T2790 T2791 nummod °,C.
R2507 T2791 T2788 pobj C.,at
R2633 T2945 T2960 nsubjpass Animals,housed
R2634 T2946 T2945 punct ",",Animals
R2635 T2947 T2952 nmod Chemicals,rats
R2636 T2948 T2947 cc and,Chemicals
R2637 T2949 T2947 conj Diet,Chemicals
R2638 T2950 T2951 compound Male,Wistar
R2639 T2951 T2947 conj Wistar,Chemicals
R2640 T2952 T2945 appos rats,Animals
R2641 T2953 T2952 punct (,rats
R2642 T2954 T2957 nummod 150,weight
R2643 T2955 T2957 amod g,weight
R2644 T2956 T2957 amod average,weight
R2645 T2957 T2952 appos weight,rats
R2646 T2958 T2952 punct ),rats
R2668 T2980 T2981 compound drinking,water
R2670 T2982 T2985 punct (,w/v
R2671 T2983 T2984 nummod 5,%
R2672 T2984 T2985 compound %,w/v
R2673 T2985 T2977 parataxis w/v,administered
R2674 T2986 T2985 punct ),w/v
R2675 T2987 T2977 prep for,administered
R2676 T2988 T2989 nummod 55,days
R2677 T2989 T2987 pobj days,for
R2678 T2990 T2989 prep to,days
R2679 T2991 T2992 nummod 2,groups
R2680 T2992 T2990 pobj groups,to
R2681 T2993 T2992 prep of,groups
R2682 T2994 T2995 nummod six,animals
R2683 T2995 T2993 pobj animals,of
R2684 T2996 T2992 npadvmod each,groups
R2685 T2997 T2977 punct .,administered
R2686 T2998 T3000 det The,group
R2687 T2999 T3000 amod first,group
R2688 T3000 T3001 nsubj group,received
R2689 T3001 T3001 ROOT received,received
R2690 T3002 T3006 det the,carrageenan
R2691 T3003 T3005 amod low,weight
R2692 T3004 T3005 amod molecular,weight
R2693 T3005 T3006 compound weight,carrageenan
R2694 T3006 T3001 dobj carrageenan,received
R2695 T3007 T3006 punct (,carrageenan
R2696 T3008 T3010 nummod 10,dCGN
R2697 T3009 T3010 compound kDa,dCGN
R2698 T3010 T3006 appos dCGN,carrageenan
R2699 T3011 T3010 punct ),dCGN
R2700 T3012 T3006 cc and,carrageenan
R2701 T3013 T3014 det the,second
R2702 T3014 T3015 nsubj second,received
R2703 T3015 T3001 conj received,received
R2704 T3016 T3020 det the,carrageenan
R2705 T3017 T3019 nmod medium,weight
R2706 T3018 T3019 amod molecular,weight
R2707 T3019 T3020 compound weight,carrageenan
R2708 T3020 T3015 dobj carrageenan,received
R2709 T3021 T3020 punct (,carrageenan
R2710 T3022 T3024 nummod 40,dCGN
R2711 T3023 T3024 compound kDa,dCGN
R2712 T3024 T3020 appos dCGN,carrageenan
R2713 T3025 T3024 punct ),dCGN
R2714 T3026 T3015 punct .,received
R2715 T3027 T3029 det An,group
R2716 T3028 T3029 amod additional,group
R2717 T3029 T3034 nsubjpass group,maintained
R2718 T3030 T3029 prep of,group
R2719 T3031 T3032 nummod four,rats
R2720 T3032 T3030 pobj rats,of
R2721 T3033 T3034 auxpass were,maintained
R2722 T3034 T3034 ROOT maintained,maintained
R2723 T3035 T3034 prep on,maintained
R2724 T3036 T3038 amod regular,water
R2725 T3037 T3038 compound tap,water
R2726 T3038 T3035 pobj water,on
R2727 T3039 T3041 punct (,group
R2728 T3040 T3041 compound control,group
R2729 T3041 T3038 appos group,water
R3147 T3503 T3507 nsubjpass segment,opened
R3148 T3504 T3503 prep of,segment
R3149 T3505 T3504 pobj colon,of
R3150 T3506 T3507 auxpass was,opened
R3151 T3507 T3507 ROOT opened,opened
R3152 T3508 T3507 advmod longitudinally,opened
R3153 T3509 T3508 cc and,longitudinally
R3154 T3510 T3508 conj macroscopic,longitudinally
R3155 T3511 T3510 cc and,macroscopic
R3156 T3512 T3510 conj histological,macroscopic
R3157 T3513 T3517 nsubjpass scores,recorded
R3158 T3514 T3513 prep of,scores
R3159 T3515 T3514 pobj inflammation,of
R3160 T3516 T3517 auxpass were,recorded
R3225 T3581 T3580 prep of,level
R3226 T3582 T3581 pobj MPO,of
R3227 T3583 T3580 punct ",",level
R3228 T3584 T3585 advmod mainly,expressed
R3229 T3585 T3580 acl expressed,level
R3230 T3586 T3585 agent by,expressed
R3231 T3587 T3586 pobj neutrophils,by
R3232 T3588 T3589 punct ",",indicates
R3233 T3589 T3589 ROOT indicates,indicates
R3940 T4386 T4389 compound Analysis,Monocytes
R3941 T4387 T4388 compound Peripheral,Blood
R3942 T4388 T4389 compound Blood,Monocytes
R3943 T4389 T4394 nsubjpass Monocytes,exposed
R3944 T4390 T4389 cc or,Monocytes
R3945 T4391 T4392 nummod THP-1,cells
R3946 T4392 T4389 conj cells,Monocytes
R3947 T4393 T4394 auxpass were,exposed
R3948 T4394 T4394 ROOT exposed,exposed
R3949 T4395 T4396 aux to,complete
R3950 T4396 T4394 xcomp complete,exposed
R3951 T4397 T4396 dobj medium,complete
R3952 T4398 T4396 prep in,complete
R4625 T5162 T5163 compound CAGATAGATGGGCTCATACC-3,′
R4626 T5163 T5156 conj ′,′
R4627 T5164 T5143 punct .,amplified
R4628 T5165 T5169 nsubjpass cDNA,amplified
R4629 T5166 T5169 prep for,amplified
R4630 T5167 T5166 pobj ICAM-1,for
R4631 T5168 T5169 auxpass was,amplified
R4632 T5169 T5169 ROOT amplified,amplified
R4633 T5170 T5169 prep for,amplified
R4634 T5171 T5172 nummod 35,cycles
R4635 T5172 T5170 pobj cycles,for
R4636 T5173 T5169 advcl using,amplified

UBERON-AE

Id Subject Object Predicate Lexical cue
T3100 2817-2820 http://purl.obolibrary.org/obo/UBERON_0000970 denotes eye
T3101 2960-2965 http://purl.obolibrary.org/obo/UBERON_0001155 denotes colon
T3102 3028-3033 http://purl.obolibrary.org/obo/UBERON_0001155 denotes colon
T3103 3423-3428 http://purl.obolibrary.org/obo/UBERON_0001155 denotes colon
T3104 3720-3725 http://purl.obolibrary.org/obo/UBERON_0001155 denotes colon
T3105 3117-3127 http://purl.obolibrary.org/obo/UBERON_0000160 denotes intestinal
T3665 4057-4063 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
T3666 4324-4329 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T3667 4379-4384 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T4232 5584-5589 http://purl.obolibrary.org/obo/UBERON_0000178 denotes Blood
T4233 5814-5819 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T4578 6549-6555 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
T5473 10351-10355 http://purl.obolibrary.org/obo/UBERON_0001913 denotes milk
T3106 3232-3242 http://purl.obolibrary.org/obo/UBERON_0000160 denotes intestinal
T3107 3653-3663 http://purl.obolibrary.org/obo/UBERON_0000160 denotes intestinal
T3108 3922-3932 http://purl.obolibrary.org/obo/UBERON_0000160 denotes intestinal
T3109 3653-3670 http://purl.obolibrary.org/obo/UBERON_0001242 denotes intestinal mucosa
T3110 3922-3939 http://purl.obolibrary.org/obo/UBERON_0001242 denotes intestinal mucosa
T3111 3664-3670 http://purl.obolibrary.org/obo/UBERON_0000344 denotes mucosa
T3112 3933-3939 http://purl.obolibrary.org/obo/UBERON_0000344 denotes mucosa

sentences

Id Subject Object Predicate Lexical cue
T2051 23-58 Sentence denotes Preparation of Degraded Carrageenan
T2052 59-327 Sentence denotes Two preparations of degraded carrageenan with low, (∼10 kDa; C10), and medium, (∼40 kDa; C40) molecular weight were prepared from native iota-carrageenan extracted from Euchema spinosum (generously provided by Sanofi Biosystems Industry, Boulogne-Billancourt, France).
T2053 328-563 Sentence denotes Native carrageenan was dissolved in distilled water (5% w/v) under vigorous stirring and heated to 60°C. Then, the carrageenan solution was submitted to two different treatments to obtain both low and medium molecular weight fractions.
T2054 564-862 Sentence denotes Briefly, for the low molecular weight fraction, carrageenan solution was hydrolyzed with 0.3% (v/v) concentrated sulphuric acid for 15 min at 80°C. After neutralization with NaOH 4N, the solution was ultra filtered through a hollow fibre cartridge with MW cut-off 5 kDa, (Amicon Inc, Beverly, USA).
T2055 863-1087 Sentence denotes For the medium molecular weight fraction, the carrageenan solution was hydrolyzed with 0.3% (v/v) concentrated sulphuric acid for 30 min at 60°C. After neutralization, the supernatant was ultra filtered (MW cut-off 100 kDa).
T2056 1088-1163 Sentence denotes The filtrate was submitted to a second ultra filtration (MW cut-off 5 kDa).
T2057 1164-1314 Sentence denotes Both preparations of dCGN were precipitated with 4 volumes of 95% ethanol, dried at room temperature and ground to small particles (1 mm in diameter).
T2058 1315-1543 Sentence denotes Using gel-permeation chromatography in combination with light scattering measurements (see Viebke et al. [21]), it was confirmed that the low fraction had an average molecular weight of 10 kDa, and the medium fraction of 40 kDa.
T2059 1544-1660 Sentence denotes The sulphate content of polysaccharides in both fractions was measured following the method of Quemener et al. [22].
T2060 1661-1785 Sentence denotes Finally, the absence of polysaccharide structure modifications in the two fractions was confirmed using 2H-NMR spectroscopy.
T2061 1786-1916 Sentence denotes The absence of LPS contamination in the two fractions was confirmed using the e-Toxate® kit (Sigma, St Quentin Fallavier, France).
T2062 1917-2019 Sentence denotes Before use in cell culture, the two fractions were dissolved in complete medium during 30 min at 56°C.
T2810 2021-2048 Sentence denotes Animals, Chemicals and Diet
T2811 2049-2183 Sentence denotes Male Wistar rats (150 g average weight) were housed under standard conditions and fed ad libitum with standard rodent laboratory chow.
T2812 2184-2304 Sentence denotes Degraded iota-carrageenans were administered in the drinking water (5% w/v) for 55 days to 2 groups of six animals each.
T2813 2305-2459 Sentence denotes The first group received the low molecular weight carrageenan (10 kDa dCGN) and the second received the medium molecular weight carrageenan (40 kDa dCGN).
T2814 2460-2546 Sentence denotes An additional group of four rats were maintained on regular tap water (control group).
T2815 2547-2660 Sentence denotes To increase palatability 0.2% sucrose was added to the drinking water of all groups (Van der Waaji et al., [23]).
T2816 2661-2709 Sentence denotes Fresh carrageenan solutions were prepared daily.
T3117 2711-2732 Sentence denotes Evaluation of Colitis
T3118 2733-2877 Sentence denotes Body weight, liquid and food consumption, diarrhea and rectal bleeding (detected by eye inspection) were recorded throughout the feeding period.
T3119 2878-2941 Sentence denotes After 55 days, animals were sacrificed by cervical dislocation.
T3120 2942-3016 Sentence denotes The length of the colon was measured as described by Okayashu et al. [24].
T3121 3017-3134 Sentence denotes Then, each colon was ligated in sections of 2 cm and 1 to 2 ml of 10% formalin was infused into the intestinal lumen.
T3122 3135-3208 Sentence denotes The moderately distended segment was sectioned and fixed in 10% formalin.
T3123 3209-3276 Sentence denotes The following day, the intestinal content was removed by vortexing.
T3124 3277-3366 Sentence denotes The fixed segment was kept in 10% formalin at 4°C until the paraffin embedding procedure.
T3125 3367-3560 Sentence denotes To evaluate the degree of inflammation, this segment of colon was opened longitudinally and macroscopic and histological scores of inflammation were recorded as previously described [25], [26].
T3126 3561-3671 Sentence denotes The toluidine blue staining was used for identification of sulphated polysaccharides in the intestinal mucosa.
T3127 3672-3814 Sentence denotes On the day of sacrifice, a fresh sample of each colon (50 mg) was collected for myeloperoxidase (MPO) assay according to Krawisz et al., [27].
T3128 3815-3940 Sentence denotes The level of MPO, mainly expressed by neutrophils, indicates the rate of recruitment of neutrophils to the intestinal mucosa.
T3129 3941-4038 Sentence denotes One unit of MPO activity corresponds to the degradation of 1 µmol of peroxide per minute at 25°C.
T3668 4040-4052 Sentence denotes Cell Culture
T3669 4053-4127 Sentence denotes All tissue culture reagents were from Invitrogen (Cergy Pontoise, France).
T3670 4128-4306 Sentence denotes THP-1 human monocytic cells were maintained in RPMI-1640 supplemented with 10% FCS, 2 mM L -glutamine, 50 U/ml penicillin and 50 mg/ml streptomycin at 37°C in a 5% CO2 incubator.
T3671 4307-4420 Sentence denotes Human peripheral blood mononuclear cells were obtained from heparinized blood by Ficoll-Hypaque density gradient.
T3672 4421-4499 Sentence denotes Monocytes were then isolated by adherence to culture flasks as described [28].
T3673 4500-4596 Sentence denotes For cell aggregation, monocytes were cultured in the presence or absence of C10 or C40 for 72 h.
T3674 4597-4715 Sentence denotes Cell colonies were monitored under an inverted phase contrast microscope coupled through a video camera to a computer.
T3675 4716-4834 Sentence denotes In some wells, neutralizing monoclonal antibody to ICAM-1 (2.5 µg/ml) (Tebu, Le Perray en Yvelines, France) was added.
T3981 4836-4855 Sentence denotes Cell Cycle Analysis
T3982 4856-5136 Sentence denotes THP-1 cells in exponential growth phase were exposed to complete medium in the presence or absence of carrageenans for 24 h before being stained with propidium iodide using the DNA-Prep Coulter kit according to the manufacturer's instruction (Beckman-Coulter, Villepinte, France).
T3983 5137-5227 Sentence denotes Cell DNA content was then analyzed by flow cytometry using an EPICS XL2 (Beckman-Coulter).
T3984 5228-5385 Sentence denotes Raw data for the distribution of DNA content of 30,000 cells retrieved from the cytometer were expressed as the percentage of G0/G1 through G2/M populations.
T3985 5386-5530 Sentence denotes Multicycle AV software (Phoenix Flow Systems, San Diego, CA) was used to generate DNA content frequency histograms and facilitate data analysis.
T4234 5532-5572 Sentence denotes Cell Surface Antigen Expression Analysis
T4235 5573-5698 Sentence denotes Peripheral Blood Monocytes or THP-1 cells were exposed to complete medium in the presence or absence of carrageenan for 36 h.
T4236 5699-5855 Sentence denotes After two washes in PBS without Ca2+ and Mg2+, cells were incubated in PBS containing 0.1% gelatin and 8% AB human serum to prevent binding to Fc receptors.
T4237 5856-5931 Sentence denotes Then, 5×105 cells were incubated with primary antibodies at 4°C for 30 min.
T4238 5932-6066 Sentence denotes Two other washes in PBS preceded incubation with FITC-conjugated goat antibody anti-mouse IgG diluted 1/1000 at 4°C for 30 min (Tebu).
T4239 6067-6170 Sentence denotes After two additional washes, analysis of stained cells was performed on an EPICS XL2 (Beckman-Coulter).
T4240 6171-6258 Sentence denotes The cell population was gated according to its forward and wide-angle light scattering.
T4241 6259-6339 Sentence denotes Data were expressed as mean relative fluorescence intensity (MFI) of 3000 cells.
T4583 6341-6362 Sentence denotes TNF Activity Bioassay
T4584 6363-6517 Sentence denotes Monocytes or THP-1 cells were cultured with or without different concentrations of CGNs or LPS (Salmonella typhosa, Sigma) for 24 h or the indicated time.
T4585 6518-6641 Sentence denotes Biologically active TNF-α/β in tissue culture supernatant was measured using the WEHI 164 clone 13-cell killing assay [29].
T4586 6642-6684 Sentence denotes TNF concentrations are expressed as pg/ml.
T4745 6686-6701 Sentence denotes RT-PCR Analysis
T4746 6702-6894 Sentence denotes Total RNA from monocytes was isolated using TRIzol Reagent™ (Invitrogen). cDNA was generated on 1 µg of total RNA in a reaction volume of 20 µl, using M-MLV reverse transcriptase (Invitrogen).
T4747 6895-6996 Sentence denotes PCR was done in the linear range of amplification (determined for each primer pair-cDNA combination).
T4748 6997-7248 Sentence denotes Standard PCR reactions were performed with 1 µl of the cDNA solution, 50 µM of each primer solution, 10 mM of each dNTP, 25 mM MgCl2, 10X Goldstar DNA polymerase reaction buffer, and 0.5 units of Goldstar DNA polymerase (Eurogentec, Seraing, Belgium).
T4749 7249-7821 Sentence denotes First PCR cycle consisted of 1 min at 92°C, 1 min at 58°C and 1 min at 72°C; then each PCR cycle consisted of 40 sec at 92°C, 40 sec at 58°C and 50 sec at 72°C. cDNA for β-actin was amplified for 28 cycles using the oligos: sense 5′-GGCATCGTGATGGACTCCG-3′ and antisense 5′GCTGGAAGGTGGACAGCGA-3′. cDNA for TNF-α was amplified for 35 cycles using the oligos: sense 5′-AAGCCTGTAGCCCATGTTGT-3′ and antisense 5′-CAGATAGATGGGCTCATACC-3′. cDNA for ICAM-1 was amplified for 35 cycles using the oligos sense 5′-GTAGCAGCCGCAGTCATAATGG-3′ and antisense 5′-A TGCTGTTGTATCTGACTGAGG-3′.
T5235 7823-7862 Sentence denotes NF-kB Transcription Reporter Gene Assay
T5236 7863-8102 Sentence denotes The plasmid 3XMHC-luc (a generous gift from Drs. J. Westwick and D.A. Brenner, University of North Carolina, Chapel Hill) contains three copies of NF-κB-responsive element from the MHC class I locus, placed upstream of the luciferase gene.
T5237 8103-8276 Sentence denotes Human monocytic THP-1 cells were transiently transfected as previously described [30], and then cultured for 4 h alone or with increasing concentration of either C10 or C40.
T5238 8277-8376 Sentence denotes Luciferase activity was determined using a luminometer (Monolight 2010 Luminometer, Ann Arbor, MI).
T5475 8378-8399 Sentence denotes Western Blot Analysis
T5476 8400-8499 Sentence denotes THP-1 cells were stimulated for various lengths of time with 0.1 mg/ml C10 or C40, or 10 µg/ml LPS.
T5477 8500-8647 Sentence denotes Cells were then pelleted, washed and homogenised in lysis buffer (10 mM Hepes, pH 7.9, 150 mM NaCl, 1 mM EDTA, 0.6% NP-40, and 0.5 mM PMSF) on ice.
T5478 8648-8755 Sentence denotes Homogenates were sonicated, centrifuged at 10,000 rpm to remove cellular debris, and supernatant collected.
T5479 8756-8830 Sentence denotes Protein concentration was determined using the DC Protein Assay (Bio-Rad).
T5480 8831-8972 Sentence denotes Proteins in samples (15 µg total proteins) were resolved in a denaturing 12% polyacrylamide gel and transferred to a nitrocellulose membrane.
T5481 8973-9176 Sentence denotes I-κBα protein was detected using a rabbit polyclonal antibody (Santa Cruz Biotechnology, CA) followed by a horseradish peroxidase-coupled goat polyclonal antibody against rabbit Ig (Caltag Laboratories).
T5482 9177-9334 Sentence denotes Finally, IκB bands were revealed using the ECL™ detection system (Amersham Pharmacia Biotech, Les Ullis, France) according to the manufacturers' instruction.
T5483 9335-9397 Sentence denotes Antibody to α-Tubulin (Santa Cruz) was use as loading control.
T5484 9398-9557 Sentence denotes For nuclear NF-κB, THP-1 cells were stimulated with 1 mg/ml C10 or C40 for 30 minutes at 37°C. Cells were then pelleted and nuclei separated as described [31].
T5485 9558-9835 Sentence denotes Nuclei were washed and homogenized directly in loading (Laemli) buffer and heated for 5 minutes at 100°C. Proteins in samples were resolved in a denaturing 8% polyacrylamide gel and transferred to a polyvinylidine fluoride (PVDF) membrane (Immobilon-P; Millipore, Bedford, MA).
T5486 9836-9931 Sentence denotes Membranes were incubated in blocking buffer (1% BSA, in PBS) for two hours at room temperature.
T5487 9932-10029 Sentence denotes Membranes were subsequently probed with the corresponding antibody in blocking buffer, overnight.
T5488 10030-10168 Sentence denotes Rabbit polyclonal antibody anti-NF-κB p50 subunit (# sc-114) or anti-NF-κB p65 subunit (# sc-109) from Santa Cruz Biotechnology were used.
T5489 10169-10413 Sentence denotes Membranes were washed six times in PBS with 0.05% Tween 20, 5 minutes each time, and incubated with a 1/3000 dilution of HRP-conjugated F(ab')2 goat anti-rabbit IgG in 5% nonfat dry milk and 0.05% Tween 20 in PBS for 1 hour at room temperature.
T5490 10414-10629 Sentence denotes After washing six more times in PBS with 0.05% Tween 20, antibody-reactive proteins were detected using a chemiluminescence substrate (SuperSignal; Pierce, Rockford, IL) according to the manufacturer's instructions.
T5491 10630-10764 Sentence denotes To confirm that equivalent amounts of protein were loaded in each line, membranes were also Western blotted for ERK as described [32].
T6463 10766-10812 Sentence denotes Analysis of NF-κB Activation by Flow Cytometry
T6464 10813-10891 Sentence denotes Nuclear activation of NF−κΒ by flow cytometry was performed as described [31].
T6517 10893-10913 Sentence denotes Statistical Analysis
T6518 10914-10994 Sentence denotes The results were expressed as the mean value ± S.E.M. of individual experiments.
T6519 10995-11133 Sentence denotes The statistical significance of the differences between mean values was assessed by the Student's t-test and analysis of variance (ANOVA).
T39 0-21 Sentence denotes Materials and Methods
T40 23-58 Sentence denotes Preparation of Degraded Carrageenan
T41 59-327 Sentence denotes Two preparations of degraded carrageenan with low, (∼10 kDa; C10), and medium, (∼40 kDa; C40) molecular weight were prepared from native iota-carrageenan extracted from Euchema spinosum (generously provided by Sanofi Biosystems Industry, Boulogne-Billancourt, France).
T42 328-432 Sentence denotes Native carrageenan was dissolved in distilled water (5% w/v) under vigorous stirring and heated to 60°C.
T43 433-563 Sentence denotes Then, the carrageenan solution was submitted to two different treatments to obtain both low and medium molecular weight fractions.
T44 564-711 Sentence denotes Briefly, for the low molecular weight fraction, carrageenan solution was hydrolyzed with 0.3% (v/v) concentrated sulphuric acid for 15 min at 80°C.
T45 712-862 Sentence denotes After neutralization with NaOH 4N, the solution was ultra filtered through a hollow fibre cartridge with MW cut-off 5 kDa, (Amicon Inc, Beverly, USA).
T46 863-1008 Sentence denotes For the medium molecular weight fraction, the carrageenan solution was hydrolyzed with 0.3% (v/v) concentrated sulphuric acid for 30 min at 60°C.
T47 1009-1087 Sentence denotes After neutralization, the supernatant was ultra filtered (MW cut-off 100 kDa).
T48 1088-1163 Sentence denotes The filtrate was submitted to a second ultra filtration (MW cut-off 5 kDa).
T49 1164-1314 Sentence denotes Both preparations of dCGN were precipitated with 4 volumes of 95% ethanol, dried at room temperature and ground to small particles (1 mm in diameter).
T50 1315-1543 Sentence denotes Using gel-permeation chromatography in combination with light scattering measurements (see Viebke et al. [21]), it was confirmed that the low fraction had an average molecular weight of 10 kDa, and the medium fraction of 40 kDa.
T51 1544-1660 Sentence denotes The sulphate content of polysaccharides in both fractions was measured following the method of Quemener et al. [22].
T52 1661-1785 Sentence denotes Finally, the absence of polysaccharide structure modifications in the two fractions was confirmed using 2H-NMR spectroscopy.
T53 1786-1916 Sentence denotes The absence of LPS contamination in the two fractions was confirmed using the e-Toxate® kit (Sigma, St Quentin Fallavier, France).
T54 1917-2019 Sentence denotes Before use in cell culture, the two fractions were dissolved in complete medium during 30 min at 56°C.
T55 2021-2048 Sentence denotes Animals, Chemicals and Diet
T56 2049-2183 Sentence denotes Male Wistar rats (150 g average weight) were housed under standard conditions and fed ad libitum with standard rodent laboratory chow.
T57 2184-2304 Sentence denotes Degraded iota-carrageenans were administered in the drinking water (5% w/v) for 55 days to 2 groups of six animals each.
T58 2305-2459 Sentence denotes The first group received the low molecular weight carrageenan (10 kDa dCGN) and the second received the medium molecular weight carrageenan (40 kDa dCGN).
T59 2460-2546 Sentence denotes An additional group of four rats were maintained on regular tap water (control group).
T60 2547-2660 Sentence denotes To increase palatability 0.2% sucrose was added to the drinking water of all groups (Van der Waaji et al., [23]).
T61 2661-2709 Sentence denotes Fresh carrageenan solutions were prepared daily.
T62 2711-2732 Sentence denotes Evaluation of Colitis
T63 2733-2877 Sentence denotes Body weight, liquid and food consumption, diarrhea and rectal bleeding (detected by eye inspection) were recorded throughout the feeding period.
T64 2878-2941 Sentence denotes After 55 days, animals were sacrificed by cervical dislocation.
T65 2942-3016 Sentence denotes The length of the colon was measured as described by Okayashu et al. [24].
T66 3017-3134 Sentence denotes Then, each colon was ligated in sections of 2 cm and 1 to 2 ml of 10% formalin was infused into the intestinal lumen.
T67 3135-3208 Sentence denotes The moderately distended segment was sectioned and fixed in 10% formalin.
T68 3209-3276 Sentence denotes The following day, the intestinal content was removed by vortexing.
T69 3277-3366 Sentence denotes The fixed segment was kept in 10% formalin at 4°C until the paraffin embedding procedure.
T70 3367-3560 Sentence denotes To evaluate the degree of inflammation, this segment of colon was opened longitudinally and macroscopic and histological scores of inflammation were recorded as previously described [25], [26].
T71 3561-3671 Sentence denotes The toluidine blue staining was used for identification of sulphated polysaccharides in the intestinal mucosa.
T72 3672-3814 Sentence denotes On the day of sacrifice, a fresh sample of each colon (50 mg) was collected for myeloperoxidase (MPO) assay according to Krawisz et al., [27].
T73 3815-3940 Sentence denotes The level of MPO, mainly expressed by neutrophils, indicates the rate of recruitment of neutrophils to the intestinal mucosa.
T74 3941-4038 Sentence denotes One unit of MPO activity corresponds to the degradation of 1 µmol of peroxide per minute at 25°C.
T75 4040-4052 Sentence denotes Cell Culture
T76 4053-4127 Sentence denotes All tissue culture reagents were from Invitrogen (Cergy Pontoise, France).
T77 4128-4306 Sentence denotes THP-1 human monocytic cells were maintained in RPMI-1640 supplemented with 10% FCS, 2 mM L -glutamine, 50 U/ml penicillin and 50 mg/ml streptomycin at 37°C in a 5% CO2 incubator.
T78 4307-4420 Sentence denotes Human peripheral blood mononuclear cells were obtained from heparinized blood by Ficoll-Hypaque density gradient.
T79 4421-4499 Sentence denotes Monocytes were then isolated by adherence to culture flasks as described [28].
T80 4500-4596 Sentence denotes For cell aggregation, monocytes were cultured in the presence or absence of C10 or C40 for 72 h.
T81 4597-4715 Sentence denotes Cell colonies were monitored under an inverted phase contrast microscope coupled through a video camera to a computer.
T82 4716-4834 Sentence denotes In some wells, neutralizing monoclonal antibody to ICAM-1 (2.5 µg/ml) (Tebu, Le Perray en Yvelines, France) was added.
T83 4836-4855 Sentence denotes Cell Cycle Analysis
T84 4856-5136 Sentence denotes THP-1 cells in exponential growth phase were exposed to complete medium in the presence or absence of carrageenans for 24 h before being stained with propidium iodide using the DNA-Prep Coulter kit according to the manufacturer's instruction (Beckman-Coulter, Villepinte, France).
T85 5137-5227 Sentence denotes Cell DNA content was then analyzed by flow cytometry using an EPICS XL2 (Beckman-Coulter).
T86 5228-5385 Sentence denotes Raw data for the distribution of DNA content of 30,000 cells retrieved from the cytometer were expressed as the percentage of G0/G1 through G2/M populations.
T87 5386-5530 Sentence denotes Multicycle AV software (Phoenix Flow Systems, San Diego, CA) was used to generate DNA content frequency histograms and facilitate data analysis.
T88 5532-5572 Sentence denotes Cell Surface Antigen Expression Analysis
T89 5573-5698 Sentence denotes Peripheral Blood Monocytes or THP-1 cells were exposed to complete medium in the presence or absence of carrageenan for 36 h.
T90 5699-5855 Sentence denotes After two washes in PBS without Ca2+ and Mg2+, cells were incubated in PBS containing 0.1% gelatin and 8% AB human serum to prevent binding to Fc receptors.
T91 5856-5931 Sentence denotes Then, 5×105 cells were incubated with primary antibodies at 4°C for 30 min.
T92 5932-6066 Sentence denotes Two other washes in PBS preceded incubation with FITC-conjugated goat antibody anti-mouse IgG diluted 1/1000 at 4°C for 30 min (Tebu).
T93 6067-6170 Sentence denotes After two additional washes, analysis of stained cells was performed on an EPICS XL2 (Beckman-Coulter).
T94 6171-6258 Sentence denotes The cell population was gated according to its forward and wide-angle light scattering.
T95 6259-6339 Sentence denotes Data were expressed as mean relative fluorescence intensity (MFI) of 3000 cells.
T96 6341-6362 Sentence denotes TNF Activity Bioassay
T97 6363-6517 Sentence denotes Monocytes or THP-1 cells were cultured with or without different concentrations of CGNs or LPS (Salmonella typhosa, Sigma) for 24 h or the indicated time.
T98 6518-6641 Sentence denotes Biologically active TNF-α/β in tissue culture supernatant was measured using the WEHI 164 clone 13-cell killing assay [29].
T99 6642-6684 Sentence denotes TNF concentrations are expressed as pg/ml.
T100 6686-6701 Sentence denotes RT-PCR Analysis
T101 6702-6894 Sentence denotes Total RNA from monocytes was isolated using TRIzol Reagent™ (Invitrogen). cDNA was generated on 1 µg of total RNA in a reaction volume of 20 µl, using M-MLV reverse transcriptase (Invitrogen).
T102 6895-6996 Sentence denotes PCR was done in the linear range of amplification (determined for each primer pair-cDNA combination).
T103 6997-7248 Sentence denotes Standard PCR reactions were performed with 1 µl of the cDNA solution, 50 µM of each primer solution, 10 mM of each dNTP, 25 mM MgCl2, 10X Goldstar DNA polymerase reaction buffer, and 0.5 units of Goldstar DNA polymerase (Eurogentec, Seraing, Belgium).
T104 7249-7821 Sentence denotes First PCR cycle consisted of 1 min at 92°C, 1 min at 58°C and 1 min at 72°C; then each PCR cycle consisted of 40 sec at 92°C, 40 sec at 58°C and 50 sec at 72°C. cDNA for β-actin was amplified for 28 cycles using the oligos: sense 5′-GGCATCGTGATGGACTCCG-3′ and antisense 5′GCTGGAAGGTGGACAGCGA-3′. cDNA for TNF-α was amplified for 35 cycles using the oligos: sense 5′-AAGCCTGTAGCCCATGTTGT-3′ and antisense 5′-CAGATAGATGGGCTCATACC-3′. cDNA for ICAM-1 was amplified for 35 cycles using the oligos sense 5′-GTAGCAGCCGCAGTCATAATGG-3′ and antisense 5′-A TGCTGTTGTATCTGACTGAGG-3′.
T105 7823-7862 Sentence denotes NF-kB Transcription Reporter Gene Assay
T106 7863-7911 Sentence denotes The plasmid 3XMHC-luc (a generous gift from Drs.
T107 7912-7914 Sentence denotes J.
T108 7915-7932 Sentence denotes Westwick and D.A.
T109 7933-8102 Sentence denotes Brenner, University of North Carolina, Chapel Hill) contains three copies of NF-κB-responsive element from the MHC class I locus, placed upstream of the luciferase gene.
T110 8103-8276 Sentence denotes Human monocytic THP-1 cells were transiently transfected as previously described [30], and then cultured for 4 h alone or with increasing concentration of either C10 or C40.
T111 8277-8376 Sentence denotes Luciferase activity was determined using a luminometer (Monolight 2010 Luminometer, Ann Arbor, MI).
T112 8378-8399 Sentence denotes Western Blot Analysis
T113 8400-8499 Sentence denotes THP-1 cells were stimulated for various lengths of time with 0.1 mg/ml C10 or C40, or 10 µg/ml LPS.
T114 8500-8647 Sentence denotes Cells were then pelleted, washed and homogenised in lysis buffer (10 mM Hepes, pH 7.9, 150 mM NaCl, 1 mM EDTA, 0.6% NP-40, and 0.5 mM PMSF) on ice.
T115 8648-8755 Sentence denotes Homogenates were sonicated, centrifuged at 10,000 rpm to remove cellular debris, and supernatant collected.
T116 8756-8830 Sentence denotes Protein concentration was determined using the DC Protein Assay (Bio-Rad).
T117 8831-8972 Sentence denotes Proteins in samples (15 µg total proteins) were resolved in a denaturing 12% polyacrylamide gel and transferred to a nitrocellulose membrane.
T118 8973-9176 Sentence denotes I-κBα protein was detected using a rabbit polyclonal antibody (Santa Cruz Biotechnology, CA) followed by a horseradish peroxidase-coupled goat polyclonal antibody against rabbit Ig (Caltag Laboratories).
T119 9177-9334 Sentence denotes Finally, IκB bands were revealed using the ECL™ detection system (Amersham Pharmacia Biotech, Les Ullis, France) according to the manufacturers' instruction.
T120 9335-9397 Sentence denotes Antibody to α-Tubulin (Santa Cruz) was use as loading control.
T121 9398-9492 Sentence denotes For nuclear NF-κB, THP-1 cells were stimulated with 1 mg/ml C10 or C40 for 30 minutes at 37°C.
T122 9493-9557 Sentence denotes Cells were then pelleted and nuclei separated as described [31].
T123 9558-9663 Sentence denotes Nuclei were washed and homogenized directly in loading (Laemli) buffer and heated for 5 minutes at 100°C.
T124 9664-9835 Sentence denotes Proteins in samples were resolved in a denaturing 8% polyacrylamide gel and transferred to a polyvinylidine fluoride (PVDF) membrane (Immobilon-P; Millipore, Bedford, MA).
T125 9836-9931 Sentence denotes Membranes were incubated in blocking buffer (1% BSA, in PBS) for two hours at room temperature.
T126 9932-10029 Sentence denotes Membranes were subsequently probed with the corresponding antibody in blocking buffer, overnight.
T127 10030-10168 Sentence denotes Rabbit polyclonal antibody anti-NF-κB p50 subunit (# sc-114) or anti-NF-κB p65 subunit (# sc-109) from Santa Cruz Biotechnology were used.
T128 10169-10413 Sentence denotes Membranes were washed six times in PBS with 0.05% Tween 20, 5 minutes each time, and incubated with a 1/3000 dilution of HRP-conjugated F(ab')2 goat anti-rabbit IgG in 5% nonfat dry milk and 0.05% Tween 20 in PBS for 1 hour at room temperature.
T129 10414-10629 Sentence denotes After washing six more times in PBS with 0.05% Tween 20, antibody-reactive proteins were detected using a chemiluminescence substrate (SuperSignal; Pierce, Rockford, IL) according to the manufacturer's instructions.
T130 10630-10764 Sentence denotes To confirm that equivalent amounts of protein were loaded in each line, membranes were also Western blotted for ERK as described [32].
T131 10766-10812 Sentence denotes Analysis of NF-κB Activation by Flow Cytometry
T132 10813-10891 Sentence denotes Nuclear activation of NF−κΒ by flow cytometry was performed as described [31].
T133 10893-10913 Sentence denotes Statistical Analysis
T134 10914-10994 Sentence denotes The results were expressed as the mean value ± S.E.M. of individual experiments.
T135 10995-11133 Sentence denotes The statistical significance of the differences between mean values was assessed by the Student's t-test and analysis of variance (ANOVA).

events-check-again

Id Subject Object Predicate Lexical cue
T3652 3752-3767 Protein denotes myeloperoxidase
T3653 3769-3772 Protein denotes MPO
T3654 3828-3831 Protein denotes MPO
T3655 3840-3849 Gene_expression denotes expressed
T4725 6341-6344 Protein denotes TNF
T4726 6538-6543 Protein denotes TNF-α
T4727 6544-6545 Protein denotes β
T4728 6642-6645 Protein denotes TNF
T5210 7419-7426 Protein denotes β-actin
T5211 7554-7559 Protein denotes TNF-α
T5212 7690-7696 Protein denotes ICAM-1
T5452 8086-8096 Protein denotes luciferase
T5453 8277-8287 Protein denotes Luciferase
T6440 8973-8978 Protein denotes I-κBα
R3269 T3653 T3652 equivalentTo MPO,myeloperoxidase
R3270 T3654 T3655 themeOf MPO,expressed

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T2808 1879-1884 Protein denotes Sigma
T2809 1886-1896 Protein denotes St Quentin
T3656 3752-3767 Protein denotes myeloperoxidase
T3657 3769-3772 Protein denotes MPO
T3658 3738-3747 Positive_regulation denotes collected
T3659 3738-3747 Positive_regulation denotes collected
T3660 3828-3831 Protein denotes MPO
T3661 3840-3849 Gene_expression denotes expressed
T3662 3953-3956 Protein denotes MPO
T3663 3985-3996 Protein_catabolism denotes degradation
T4230 5199-5208 Protein denotes EPICS XL2
T4558 5537-5552 Protein denotes Surface Antigen
T4559 5553-5563 Gene_expression denotes Expression
T4560 5842-5854 Protein denotes Fc receptors
T4561 5831-5838 Binding denotes binding
T4562 6022-6025 Protein denotes IgG
T4563 6142-6151 Protein denotes EPICS XL2
T4729 6341-6353 Protein denotes TNF Activity
T4730 6538-6545 Protein denotes TNF-α/β
T4731 6642-6645 Protein denotes TNF
T4732 6665-6674 Gene_expression denotes expressed
T5213 6853-6880 Protein denotes M-MLV reverse transcriptase
T5214 7135-7158 Protein denotes Goldstar DNA polymerase
T5215 7193-7216 Protein denotes Goldstar DNA polymerase
T5216 7419-7426 Protein denotes β-actin
T5217 7554-7559 Protein denotes TNF-α
T5218 7690-7696 Protein denotes ICAM-1
T5454 8010-8015 Protein denotes NF-κB
T5455 8044-8061 Protein denotes MHC class I locus
T5456 8086-8101 Protein denotes luciferase gene
T5457 8277-8287 Protein denotes Luciferase
T6515 10813-10820 Entity denotes Nuclear
T6441 8825-8828 Protein denotes Rad
T6442 8973-8986 Protein denotes I-κBα protein
T6443 9080-9102 Protein denotes horseradish peroxidase
T6444 9186-9189 Protein denotes IκB
T6445 9347-9356 Protein denotes α-Tubulin
T6446 9402-9415 Protein denotes nuclear NF-κB
T6447 10030-10061 Protein denotes Rabbit polyclonal antibody anti
T6448 10062-10067 Protein denotes NF-κB
T6449 10068-10079 Protein denotes p50 subunit
T6450 10094-10116 Protein denotes anti-NF-κB p65 subunit
T6451 10290-10333 Protein denotes HRP-conjugated F(ab')2 goat anti-rabbit IgG
T6452 10742-10745 Protein denotes ERK
T6512 10778-10783 Protein denotes NF-κB
T6513 10784-10794 Positive_regulation denotes Activation
T6514 10835-10840 Protein denotes NF−κΒ
T6516 10821-10831 Positive_regulation denotes activation
R3271 T3656 T3658 themeOf myeloperoxidase,collected
R3272 T3657 T3659 themeOf MPO,collected
R3273 T3660 T3661 themeOf MPO,expressed
R3274 T3662 T3663 themeOf MPO,degradation
R4090 T4558 T4559 themeOf Surface Antigen,Expression
R4091 T4560 T4561 themeOf Fc receptors,binding
R4212 T4731 T4732 themeOf TNF,expressed
R5797 T6512 T6513 themeOf NF-κB,Activation
R5798 T6514 T6516 themeOf NF−κΒ,activation
R5799 T6515 T6516 Site Nuclear,activation

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T3114 3769-3772 Protein denotes MPO
T3115 3828-3831 Protein denotes MPO
T3116 3840-3849 Gene_expression denotes expressed
T4581 6544-6545 Protein denotes β
T4582 6642-6645 Protein denotes TNF
T5474 8973-8978 Protein denotes I-κBα
T4579 6341-6344 Protein denotes TNF
T4580 6538-6543 Protein denotes TNF-α
T5233 8086-8096 Protein denotes luciferase
T5234 8277-8287 Protein denotes Luciferase
T4742 7419-7426 Protein denotes β-actin
T4743 7554-7559 Protein denotes TNF-α
T4744 7690-7696 Protein denotes ICAM-1
T3113 3752-3767 Protein denotes myeloperoxidase
R2770 T3114 T3113 equivalentTo MPO,myeloperoxidase
R2771 T3115 T3116 themeOf MPO,expressed

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T2944 2632-2635 Q15025 denotes Van
T3815 4767-4773 P05362 denotes ICAM-1
T4381 6287-6295 Q04864 denotes relative
T4644 6341-6344 P01375 denotes TNF
T4645 6538-6541 P01375 denotes TNF
T4646 6538-6543 P01375 denotes TNF-α
T4647 6642-6645 P01375 denotes TNF
T4947 7419-7426 P60709 denotes β-actin
T4948 7554-7557 P01375 denotes TNF
T4949 7554-7559 P01375 denotes TNF-α
T4950 7690-7696 P05362 denotes ICAM-1
T5333 8086-8096 P08659 denotes luciferase
T5334 8277-8287 P08659 denotes Luciferase
T5928 8579-8581 P0A7Z4 denotes pH
T5929 10068-10071 P19838 denotes p50
T5930 10068-10079 P19838 denotes p50 subunit
T5931 10105-10108 Q04206 denotes p65
T5932 10105-10108 P21579 denotes p65

test2

Id Subject Object Predicate Lexical cue
T3096 3752-3767 Protein denotes myeloperoxidase
T3097 3769-3772 Protein denotes MPO
T3098 3828-3831 Protein denotes MPO
T3099 3840-3849 Gene_expression denotes expressed
T4231 5831-5838 Binding denotes binding
T4574 6341-6344 Protein denotes TNF
T4575 6538-6543 Protein denotes TNF-α
T4576 6544-6545 Protein denotes β
T4577 6642-6645 Protein denotes TNF
T4739 7419-7426 Protein denotes β-actin
T4740 7554-7559 Protein denotes TNF-α
T4741 7690-7696 Protein denotes ICAM-1
T5231 8086-8096 Protein denotes luciferase
T5232 8277-8287 Protein denotes Luciferase
T5472 8973-8978 Protein denotes I-κBα
R2768 T3097 T3096 equivalentTo MPO,myeloperoxidase
R2769 T3098 T3099 themeOf MPO,expressed