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PMC:2800179 / 13000-15386 JSONTXT

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GO-CC

Id Subject Object Predicate Lexical cue
T6420 28-33 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6421 1045-1050 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6422 585-593 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T6423 1410-1418 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T6424 1458-1467 http://purl.obolibrary.org/obo/GO_0016020 denotes Membranes
T6425 1554-1563 http://purl.obolibrary.org/obo/GO_0016020 denotes Membranes
T6426 1791-1800 http://purl.obolibrary.org/obo/GO_0016020 denotes Membranes
T6427 2324-2333 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T6428 648-656 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6429 749-757 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6430 1612-1620 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6431 1670-1678 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6432 2093-2101 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6433 648-656 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T6434 749-757 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T6435 1612-1620 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T6436 1670-1678 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T6437 2093-2101 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody

GO-MF

Id Subject Object Predicate Lexical cue
T6413 648-656 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T6414 749-757 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T6415 1612-1620 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T6416 1670-1678 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T6417 2093-2101 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T6418 684-686 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T6419 2364-2367 http://purl.obolibrary.org/obo/GO_0004707 denotes ERK

GO-BP

Id Subject Object Predicate Lexical cue
T6409 174-179 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T6410 334-342 http://purl.obolibrary.org/obo/GO_0007349 denotes cellular
T6411 684-686 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T6412 2364-2367 http://purl.obolibrary.org/obo/GO_0004707 denotes ERK

2_test

Id Subject Object Predicate Lexical cue
20072622-19342628-93233369 2382-2384 19342628 denotes 32

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T5492 0-7 NNP denotes Western
T5493 8-12 NNP denotes Blot
T5494 13-21 NN denotes Analysis
T5495 22-27 NN denotes THP-1
T5496 28-33 NN denotes cells
T5497 34-38 VB denotes were
T5498 39-49 VB denotes stimulated
T5499 50-53 IN denotes for
T5500 54-61 JJ denotes various
T5501 62-69 NN denotes lengths
T5502 70-72 IN denotes of
T5503 73-77 NN denotes time
T5504 78-82 IN denotes with
T5505 83-86 CD denotes 0.1
T5506 87-92 NN denotes mg/ml
T5507 93-96 NN denotes C10
T5508 97-99 CC denotes or
T5509 100-103 NN denotes C40
T5510 103-104 -COMMA- denotes ,
T5511 105-107 CC denotes or
T5512 108-110 CD denotes 10
T5513 111-116 NN denotes µg/ml
T5514 117-120 NN denotes LPS
T5515 122-127 NN denotes Cells
T5516 128-132 VB denotes were
T5517 133-137 RB denotes then
T5518 138-146 VB denotes pelleted
T5519 146-147 -COMMA- denotes ,
T5520 148-154 VB denotes washed
T5521 155-158 CC denotes and
T5522 159-170 VB denotes homogenised
T5523 171-173 IN denotes in
T5524 174-179 NN denotes lysis
T5525 180-186 NN denotes buffer
T5526 187-188 -LRB- denotes (
T5527 188-190 CD denotes 10
T5528 191-193 NN denotes mM
T5529 194-199 NNP denotes Hepes
T5530 199-200 -COMMA- denotes ,
T5531 201-203 NN denotes pH
T5532 204-207 CD denotes 7.9
T5533 207-208 -COMMA- denotes ,
T5534 209-212 CD denotes 150
T5535 213-215 NN denotes mM
T5536 216-220 NN denotes NaCl
T5537 220-221 -COMMA- denotes ,
T5538 222-223 CD denotes 1
T5539 224-226 NN denotes mM
T5540 227-231 NN denotes EDTA
T5541 231-232 -COMMA- denotes ,
T5542 233-236 CD denotes 0.6
T5543 236-237 NN denotes %
T5544 238-243 NN denotes NP-40
T5545 243-244 -COMMA- denotes ,
T5546 245-248 CC denotes and
T5547 249-252 CD denotes 0.5
T5548 253-255 NN denotes mM
T5549 256-260 NN denotes PMSF
T5550 260-261 -RRB- denotes )
T5551 262-264 IN denotes on
T5552 265-268 NN denotes ice
T5553 270-281 NN denotes Homogenates
T5554 282-286 VB denotes were
T5555 287-296 VB denotes sonicated
T5556 296-297 -COMMA- denotes ,
T5557 298-309 VB denotes centrifuged
T5558 310-312 IN denotes at
T5559 313-319 CD denotes 10,000
T5560 320-323 NN denotes rpm
T5561 324-326 TO denotes to
T5562 327-333 VB denotes remove
T5563 334-342 JJ denotes cellular
T5564 343-349 NN denotes debris
T5565 349-350 -COMMA- denotes ,
T5566 351-354 CC denotes and
T5567 355-366 NN denotes supernatant
T5568 367-376 VB denotes collected
T5569 378-385 NN denotes Protein
T5570 386-399 NN denotes concentration
T5571 400-403 VB denotes was
T5572 404-414 VB denotes determined
T5573 415-420 VB denotes using
T5574 421-424 DT denotes the
T5575 425-427 NN denotes DC
T5576 428-435 NN denotes Protein
T5577 436-441 NN denotes Assay
T5578 442-443 -LRB- denotes (
T5579 443-450 NN denotes Bio-Rad
T5580 450-451 -RRB- denotes )
T5581 453-461 NN denotes Proteins
T5582 462-464 IN denotes in
T5583 465-472 NN denotes samples
T5584 473-474 -LRB- denotes (
T5585 474-476 CD denotes 15
T5586 477-479 -COLON- denotes µg
T5587 480-485 JJ denotes total
T5588 486-494 NN denotes proteins
T5589 494-495 -RRB- denotes )
T5590 496-500 VB denotes were
T5591 501-509 VB denotes resolved
T5592 510-512 IN denotes in
T5593 513-514 DT denotes a
T5594 515-525 VB denotes denaturing
T5595 526-528 CD denotes 12
T5596 528-529 NN denotes %
T5597 530-544 NN denotes polyacrylamide
T5598 545-548 NN denotes gel
T5599 549-552 CC denotes and
T5600 553-564 VB denotes transferred
T5601 565-567 TO denotes to
T5602 568-569 DT denotes a
T5603 570-584 NN denotes nitrocellulose
T5604 585-593 NN denotes membrane
T5605 595-600 JJ denotes I-κBα
T5606 601-608 NN denotes protein
T5607 609-612 VB denotes was
T5608 613-621 VB denotes detected
T5609 622-627 VB denotes using
T5610 628-629 DT denotes a
T5611 630-636 NN denotes rabbit
T5612 637-647 JJ denotes polyclonal
T5613 648-656 NN denotes antibody
T5614 657-658 -LRB- denotes (
T5615 658-663 NNP denotes Santa
T5616 664-668 NNP denotes Cruz
T5617 669-682 NNP denotes Biotechnology
T5618 682-683 -COMMA- denotes ,
T5619 684-686 NN denotes CA
T5620 686-687 -RRB- denotes )
T5621 688-696 VB denotes followed
T5622 697-699 IN denotes by
T5623 700-701 DT denotes a
T5624 702-713 JJ denotes horseradish
T5625 714-732 JJ denotes peroxidase-coupled
T5626 733-737 NN denotes goat
T5627 738-748 JJ denotes polyclonal
T5628 749-757 NN denotes antibody
T5629 758-765 IN denotes against
T5630 766-772 NN denotes rabbit
T5631 773-775 NN denotes Ig
T5632 776-777 -LRB- denotes (
T5633 777-783 NNP denotes Caltag
T5634 784-796 NNP denotes Laboratories
T5635 796-797 -RRB- denotes )
T5636 799-806 RB denotes Finally
T5637 806-807 -COMMA- denotes ,
T5638 808-811 NN denotes IκB
T5639 812-817 NN denotes bands
T5640 818-822 VB denotes were
T5641 823-831 VB denotes revealed
T5642 832-837 VB denotes using
T5643 838-841 DT denotes the
T5644 842-846 NN denotes ECL™
T5645 847-856 NN denotes detection
T5646 857-863 NN denotes system
T5647 864-865 -LRB- denotes (
T5648 865-873 NNP denotes Amersham
T5649 874-883 NNP denotes Pharmacia
T5650 884-891 NNP denotes Biotech
T5651 891-892 -COMMA- denotes ,
T5652 893-896 NNP denotes Les
T5653 897-902 NNP denotes Ullis
T5654 902-903 -COMMA- denotes ,
T5655 904-910 NNP denotes France
T5656 910-911 -RRB- denotes )
T5657 912-921 VB denotes according
T5658 922-924 TO denotes to
T5659 925-928 DT denotes the
T5660 929-942 NN denotes manufacturers
T5661 942-943 POS denotes '
T5662 944-955 NN denotes instruction
T5663 957-965 NN denotes Antibody
T5664 966-968 TO denotes to
T5665 969-978 NN denotes α-Tubulin
T5666 979-980 -LRB- denotes (
T5667 980-985 NNP denotes Santa
T5668 986-990 NNP denotes Cruz
T5669 990-991 -RRB- denotes )
T5670 992-995 VB denotes was
T5671 996-999 NN denotes use
T5672 1000-1002 IN denotes as
T5673 1003-1010 VB denotes loading
T5674 1011-1018 NN denotes control
T5675 1020-1023 IN denotes For
T5676 1024-1031 JJ denotes nuclear
T5677 1032-1037 NN denotes NF-κB
T5678 1037-1038 -COMMA- denotes ,
T5679 1039-1044 NN denotes THP-1
T5680 1045-1050 NN denotes cells
T5681 1051-1055 VB denotes were
T5682 1056-1066 VB denotes stimulated
T5683 1067-1071 IN denotes with
T5684 1072-1073 CD denotes 1
T5685 1074-1079 NN denotes mg/ml
T5686 1080-1083 NN denotes C10
T5687 1084-1086 CC denotes or
T5688 1087-1090 NN denotes C40
T5689 1091-1094 IN denotes for
T5690 1095-1097 CD denotes 30
T5691 1098-1105 NN denotes minutes
T5692 1106-1108 IN denotes at
T5693 1109-1114 NNP denotes 37°C.
T5694 1115-1120 NNP denotes Cells
T5695 1121-1125 VB denotes were
T5696 1126-1130 RB denotes then
T5697 1131-1139 VB denotes pelleted
T5698 1140-1143 CC denotes and
T5699 1144-1150 NN denotes nuclei
T5700 1151-1160 VB denotes separated
T5701 1161-1163 IN denotes as
T5702 1164-1173 VB denotes described
T5703 1174-1175 -LRB- denotes [
T5704 1175-1177 CD denotes 31
T5705 1177-1178 -RRB- denotes ]
T5706 1180-1186 NN denotes Nuclei
T5707 1187-1191 VB denotes were
T5708 1192-1198 VB denotes washed
T5709 1199-1202 CC denotes and
T5710 1203-1214 VB denotes homogenized
T5711 1215-1223 RB denotes directly
T5712 1224-1226 IN denotes in
T5713 1227-1234 VB denotes loading
T5714 1235-1236 -LRB- denotes (
T5715 1236-1242 NNP denotes Laemli
T5716 1242-1243 -RRB- denotes )
T5717 1244-1250 NN denotes buffer
T5718 1251-1254 CC denotes and
T5719 1255-1261 VB denotes heated
T5720 1262-1265 IN denotes for
T5721 1266-1267 CD denotes 5
T5722 1268-1275 NN denotes minutes
T5723 1276-1278 IN denotes at
T5724 1279-1285 NNP denotes 100°C.
T5725 1286-1294 NNP denotes Proteins
T5726 1295-1297 IN denotes in
T5727 1298-1305 NN denotes samples
T5728 1306-1310 VB denotes were
T5729 1311-1319 VB denotes resolved
T5730 1320-1322 IN denotes in
T5731 1323-1324 DT denotes a
T5732 1325-1335 VB denotes denaturing
T5733 1336-1337 CD denotes 8
T5734 1337-1338 NN denotes %
T5735 1339-1353 NN denotes polyacrylamide
T5736 1354-1357 NN denotes gel
T5737 1358-1361 CC denotes and
T5738 1362-1373 VB denotes transferred
T5739 1374-1376 TO denotes to
T5740 1377-1378 DT denotes a
T5741 1379-1393 NN denotes polyvinylidine
T5742 1394-1402 NN denotes fluoride
T5743 1403-1404 -LRB- denotes (
T5744 1404-1408 NN denotes PVDF
T5745 1408-1409 -RRB- denotes )
T5746 1410-1418 NN denotes membrane
T5747 1419-1420 -LRB- denotes (
T5748 1420-1431 NN denotes Immobilon-P
T5749 1431-1432 -COLON- denotes ;
T5750 1433-1442 NNP denotes Millipore
T5751 1442-1443 -COMMA- denotes ,
T5752 1444-1451 NNP denotes Bedford
T5753 1451-1452 -COMMA- denotes ,
T5754 1453-1455 JJ denotes MA
T5755 1455-1456 -RRB- denotes )
T5756 1458-1467 NN denotes Membranes
T5757 1468-1472 VB denotes were
T5758 1473-1482 VB denotes incubated
T5759 1483-1485 IN denotes in
T5760 1486-1494 VB denotes blocking
T5761 1495-1501 NN denotes buffer
T5762 1502-1503 -LRB- denotes (
T5763 1503-1504 CD denotes 1
T5764 1504-1505 NN denotes %
T5765 1506-1509 NN denotes BSA
T5766 1509-1510 -COMMA- denotes ,
T5767 1511-1513 IN denotes in
T5768 1514-1517 NNP denotes PBS
T5769 1517-1518 -RRB- denotes )
T5770 1519-1522 IN denotes for
T5771 1523-1526 CD denotes two
T5772 1527-1532 NN denotes hours
T5773 1533-1535 IN denotes at
T5774 1536-1540 NN denotes room
T5775 1541-1552 NN denotes temperature
T5776 1554-1563 NN denotes Membranes
T5777 1564-1568 VB denotes were
T5778 1569-1581 RB denotes subsequently
T5779 1582-1588 VB denotes probed
T5780 1589-1593 IN denotes with
T5781 1594-1597 DT denotes the
T5782 1598-1611 JJ denotes corresponding
T5783 1612-1620 NN denotes antibody
T5784 1621-1623 IN denotes in
T5785 1624-1632 VB denotes blocking
T5786 1633-1639 NN denotes buffer
T5787 1639-1640 -COMMA- denotes ,
T5788 1641-1650 RB denotes overnight
T5789 1652-1658 NN denotes Rabbit
T5790 1659-1669 JJ denotes polyclonal
T5791 1670-1678 NN denotes antibody
T5792 1679-1689 JJ denotes anti-NF-κB
T5793 1690-1693 NN denotes p50
T5794 1694-1701 NN denotes subunit
T5795 1702-1703 -LRB- denotes (
T5796 1703-1704 -SHARP- denotes #
T5797 1705-1711 NN denotes sc-114
T5798 1711-1712 -RRB- denotes )
T5799 1713-1715 CC denotes or
T5800 1716-1726 JJ denotes anti-NF-κB
T5801 1727-1730 NN denotes p65
T5802 1731-1738 NN denotes subunit
T5803 1739-1740 -LRB- denotes (
T5804 1740-1741 -SHARP- denotes #
T5805 1742-1748 NN denotes sc-109
T5806 1748-1749 -RRB- denotes )
T5807 1750-1754 IN denotes from
T5808 1755-1760 NNP denotes Santa
T5809 1761-1765 NNP denotes Cruz
T5810 1766-1779 NNP denotes Biotechnology
T5811 1780-1784 VB denotes were
T5812 1785-1789 VB denotes used
T5813 1791-1800 NN denotes Membranes
T5814 1801-1805 VB denotes were
T5815 1806-1812 VB denotes washed
T5816 1813-1816 CD denotes six
T5817 1817-1822 NN denotes times
T5818 1823-1825 IN denotes in
T5819 1826-1829 NNP denotes PBS
T5820 1830-1834 IN denotes with
T5821 1835-1839 CD denotes 0.05
T5822 1839-1840 NN denotes %
T5823 1841-1846 CD denotes Tween
T5824 1847-1849 CD denotes 20
T5825 1849-1850 -COMMA- denotes ,
T5826 1851-1852 CD denotes 5
T5827 1853-1860 NN denotes minutes
T5828 1861-1865 DT denotes each
T5829 1866-1870 NN denotes time
T5830 1870-1871 -COMMA- denotes ,
T5831 1872-1875 CC denotes and
T5832 1876-1885 VB denotes incubated
T5833 1886-1890 IN denotes with
T5834 1891-1892 DT denotes a
T5835 1893-1899 CD denotes 1/3000
T5836 1900-1908 NN denotes dilution
T5837 1909-1911 IN denotes of
T5838 1912-1926 JJ denotes HRP-conjugated
T5839 1927-1928 NN denotes F
T5840 1928-1929 -LRB- denotes (
T5841 1929-1931 NN denotes ab
T5842 1931-1932 -DQE- denotes '
T5843 1932-1933 -RRB- denotes )
T5844 1933-1934 CD denotes 2
T5845 1935-1939 NN denotes goat
T5846 1940-1951 JJ denotes anti-rabbit
T5847 1952-1955 NN denotes IgG
T5848 1956-1958 IN denotes in
T5849 1959-1960 CD denotes 5
T5850 1960-1961 NN denotes %
T5851 1962-1968 JJ denotes nonfat
T5852 1969-1972 JJ denotes dry
T5853 1973-1977 NN denotes milk
T5854 1978-1981 CC denotes and
T5855 1982-1986 CD denotes 0.05
T5856 1986-1987 NN denotes %
T5857 1988-1993 CD denotes Tween
T5858 1994-1996 CD denotes 20
T5859 1997-1999 IN denotes in
T5860 2000-2003 NNP denotes PBS
T5861 2004-2007 IN denotes for
T5862 2008-2009 CD denotes 1
T5863 2010-2014 NN denotes hour
T5864 2015-2017 IN denotes at
T5865 2018-2022 NN denotes room
T5866 2023-2034 NN denotes temperature
T5867 2036-2041 IN denotes After
T5868 2042-2049 VB denotes washing
T5869 2050-2053 CD denotes six
T5870 2054-2058 JJ denotes more
T5871 2059-2064 NN denotes times
T5872 2065-2067 IN denotes in
T5873 2068-2071 NNP denotes PBS
T5874 2072-2076 IN denotes with
T5875 2077-2081 CD denotes 0.05
T5876 2081-2082 NN denotes %
T5877 2083-2088 CD denotes Tween
T5878 2089-2091 CD denotes 20
T5879 2091-2092 -COMMA- denotes ,
T5880 2093-2110 JJ denotes antibody-reactive
T5881 2111-2119 NN denotes proteins
T5882 2120-2124 VB denotes were
T5883 2125-2133 VB denotes detected
T5884 2134-2139 VB denotes using
T5885 2140-2141 DT denotes a
T5886 2142-2159 NN denotes chemiluminescence
T5887 2160-2169 NN denotes substrate
T5888 2170-2171 -LRB- denotes (
T5889 2171-2182 NNP denotes SuperSignal
T5890 2182-2183 -COLON- denotes ;
T5891 2184-2190 NNP denotes Pierce
T5892 2190-2191 -COMMA- denotes ,
T5893 2192-2200 NNP denotes Rockford
T5894 2200-2201 -COMMA- denotes ,
T5895 2202-2204 NN denotes IL
T5896 2204-2205 -RRB- denotes )
T5897 2206-2215 VB denotes according
T5898 2216-2218 TO denotes to
T5899 2219-2222 DT denotes the
T5900 2223-2235 NN denotes manufacturer
T5901 2235-2237 POS denotes 's
T5902 2238-2250 NN denotes instructions
T5903 2252-2254 TO denotes To
T5904 2255-2262 VB denotes confirm
T5905 2263-2267 IN denotes that
T5906 2268-2278 JJ denotes equivalent
T5907 2279-2286 NN denotes amounts
T5908 2287-2289 IN denotes of
T5909 2290-2297 NN denotes protein
T5910 2298-2302 VB denotes were
T5911 2303-2309 VB denotes loaded
T5912 2310-2312 IN denotes in
T5913 2313-2317 DT denotes each
T5914 2318-2322 NN denotes line
T5915 2322-2323 -COMMA- denotes ,
T5916 2324-2333 NN denotes membranes
T5917 2334-2338 VB denotes were
T5918 2339-2343 RB denotes also
T5919 2344-2351 JJ denotes Western
T5920 2352-2359 VB denotes blotted
T5921 2360-2363 IN denotes for
T5922 2364-2367 NN denotes ERK
T5923 2368-2370 IN denotes as
T5924 2371-2380 VB denotes described
T5925 2381-2382 -LRB- denotes [
T5926 2382-2384 CD denotes 32
T5927 2384-2385 -RRB- denotes ]
R4845 T5493 T5492 arg1Of Blot,Western
R4846 T5494 T5493 arg1Of Analysis,Blot
R4847 T5496 T5495 arg1Of cells,THP-1
R4848 T5496 T5497 arg1Of cells,were
R4849 T5496 T5498 arg2Of cells,stimulated
R4850 T5498 T5497 arg2Of stimulated,were
R4851 T5498 T5499 arg1Of stimulated,for
R4852 T5501 T5499 arg2Of lengths,for
R4853 T5501 T5500 arg1Of lengths,various
R4854 T5501 T5502 arg1Of lengths,of
R4855 T5503 T5502 arg2Of time,of
R4856 T5503 T5504 arg1Of time,with
R4857 T5505 T5506 arg1Of 0.1,mg/ml
R4858 T5507 T5505 arg1Of C10,0.1
R4859 T5507 T5508 arg1Of C10,or
R4860 T5508 T5511 arg1Of or,or
R4861 T5509 T5508 arg2Of C40,or
R4862 T5511 T5504 arg2Of or,with
R4863 T5511 T5510 arg1Of or,","
R4864 T5514 T5511 arg2Of LPS,or
R4865 T5514 T5512 arg1Of LPS,10
R4866 T5514 T5513 arg1Of LPS,µg/ml
R4867 T5515 T5516 arg1Of Cells,were
R4868 T5515 T5518 arg2Of Cells,pelleted
R4869 T5515 T5520 arg2Of Cells,washed
R4870 T5515 T5522 arg2Of Cells,homogenised
R4871 T5518 T5519 arg1Of pelleted,","
R4872 T5519 T5521 arg1Of ",",and
R4873 T5520 T5519 arg2Of washed,","
R4874 T5521 T5516 arg2Of and,were
R4875 T5521 T5517 arg1Of and,then
R4876 T5522 T5521 arg2Of homogenised,and
R4877 T5522 T5523 arg1Of homogenised,in
R4878 T5525 T5523 arg2Of buffer,in
R4879 T5525 T5524 arg1Of buffer,lysis
R4880 T5525 T5526 arg1Of buffer,(
R4881 T5525 T5551 arg1Of buffer,on
R4882 T5529 T5526 arg2Of Hepes,(
R4883 T5529 T5527 arg1Of Hepes,10
R4884 T5529 T5528 arg1Of Hepes,mM
R4885 T5529 T5530 arg1Of Hepes,","
R4886 T5531 T5532 arg1Of pH,7.9
R4887 T5531 T5533 arg1Of pH,","
R4888 T5533 T5537 arg1Of ",",","
R4889 T5534 T5535 arg1Of 150,mM
R4890 T5536 T5533 arg2Of NaCl,","
R4891 T5536 T5534 arg1Of NaCl,150
R4892 T5537 T5541 arg1Of ",",","
R4893 T5538 T5539 arg1Of 1,mM
R4894 T5540 T5537 arg2Of EDTA,","
R4895 T5540 T5538 arg1Of EDTA,1
R4896 T5541 T5546 arg1Of ",",and
R4897 T5542 T5543 arg1Of 0.6,%
R4898 T5544 T5541 arg2Of NP-40,","
R4899 T5544 T5542 arg1Of NP-40,0.6
R4900 T5546 T5530 arg2Of and,","
R4901 T5546 T5545 arg1Of and,","
R4902 T5547 T5548 arg1Of 0.5,mM
R4903 T5549 T5546 arg2Of PMSF,and
R4904 T5549 T5547 arg1Of PMSF,0.5
R4905 T5550 T5526 arg3Of ),(
R4906 T5552 T5551 arg2Of ice,on
R4907 T5553 T5554 arg1Of Homogenates,were
R4908 T5553 T5555 arg2Of Homogenates,sonicated
R4909 T5553 T5557 arg2Of Homogenates,centrifuged
R4910 T5555 T5556 arg1Of sonicated,","
R4911 T5556 T5554 arg2Of ",",were
R4912 T5556 T5561 modOf ",",to
R4913 T5557 T5556 arg2Of centrifuged,","
R4914 T5557 T5558 arg1Of centrifuged,at
R4915 T5560 T5558 arg2Of rpm,at
R4916 T5560 T5559 arg1Of rpm,"10,000"
R4917 T5562 T5561 arg1Of remove,to
R4918 T5564 T5563 arg1Of debris,cellular
R4919 T5564 T5566 arg1Of debris,and
R4920 T5566 T5562 arg2Of and,remove
R4921 T5566 T5565 arg1Of and,","
R4922 T5566 T5568 arg2Of and,collected
R4923 T5567 T5566 arg2Of supernatant,and
R4924 T5570 T5569 arg1Of concentration,Protein
R4925 T5570 T5571 arg1Of concentration,was
R4926 T5570 T5572 arg2Of concentration,determined
R4927 T5572 T5571 arg2Of determined,was
R4928 T5573 T5572 arg3Of using,determined
R4929 T5577 T5573 arg2Of Assay,using
R4930 T5577 T5574 arg1Of Assay,the
R4931 T5577 T5575 arg1Of Assay,DC
R4932 T5577 T5576 arg1Of Assay,Protein
R4933 T5577 T5578 arg1Of Assay,(
R4934 T5579 T5578 arg2Of Bio-Rad,(
R4935 T5580 T5578 arg3Of ),(
R4936 T5581 T5582 arg1Of Proteins,in
R4937 T5581 T5590 arg1Of Proteins,were
R4938 T5581 T5591 arg2Of Proteins,resolved
R4939 T5581 T5600 arg2Of Proteins,transferred
R4940 T5583 T5582 arg2Of samples,in
R4941 T5583 T5584 arg1Of samples,(
R4942 T5588 T5584 arg2Of proteins,(
R4943 T5588 T5585 arg1Of proteins,15
R4944 T5588 T5586 arg1Of proteins,µg
R4945 T5588 T5587 arg1Of proteins,total
R4946 T5589 T5584 arg3Of ),(
R4947 T5591 T5592 arg1Of resolved,in
R4948 T5591 T5599 arg1Of resolved,and
R4949 T5595 T5596 arg1Of 12,%
R4950 T5598 T5592 arg2Of gel,in
R4951 T5598 T5593 arg1Of gel,a
R4952 T5598 T5594 arg1Of gel,denaturing
R4953 T5598 T5595 arg1Of gel,12
R4954 T5598 T5597 arg1Of gel,polyacrylamide
R4955 T5599 T5590 arg2Of and,were
R4956 T5600 T5599 arg2Of transferred,and
R4957 T5600 T5601 arg1Of transferred,to
R4958 T5604 T5601 arg2Of membrane,to
R4959 T5604 T5602 arg1Of membrane,a
R4960 T5604 T5603 arg1Of membrane,nitrocellulose
R4961 T5606 T5605 arg1Of protein,I-κBα
R4962 T5606 T5607 arg1Of protein,was
R4963 T5606 T5608 arg2Of protein,detected
R4964 T5606 T5609 arg1Of protein,using
R4965 T5608 T5607 arg2Of detected,was
R4966 T5609 T5608 arg3Of using,detected
R4967 T5609 T5621 modOf using,followed
R4968 T5613 T5609 arg2Of antibody,using
R4969 T5613 T5610 arg1Of antibody,a
R4970 T5613 T5611 arg1Of antibody,rabbit
R4971 T5613 T5612 arg1Of antibody,polyclonal
R4972 T5613 T5614 arg1Of antibody,(
R4973 T5617 T5614 arg2Of Biotechnology,(
R4974 T5617 T5615 arg1Of Biotechnology,Santa
R4975 T5617 T5616 arg1Of Biotechnology,Cruz
R4976 T5617 T5618 arg1Of Biotechnology,","
R4977 T5619 T5618 arg2Of CA,","
R4978 T5620 T5614 arg3Of ),(
R4979 T5628 T5621 arg1Of antibody,followed
R4980 T5628 T5622 arg2Of antibody,by
R4981 T5628 T5623 arg1Of antibody,a
R4982 T5628 T5624 arg1Of antibody,horseradish
R4983 T5628 T5625 arg1Of antibody,peroxidase-coupled
R4984 T5628 T5626 arg1Of antibody,goat
R4985 T5628 T5627 arg1Of antibody,polyclonal
R4986 T5628 T5629 arg1Of antibody,against
R4987 T5631 T5629 arg2Of Ig,against
R4988 T5631 T5630 arg1Of Ig,rabbit
R4989 T5631 T5632 arg1Of Ig,(
R4990 T5634 T5632 arg2Of Laboratories,(
R4991 T5634 T5633 arg1Of Laboratories,Caltag
R4992 T5635 T5632 arg3Of ),(
R4993 T5639 T5638 arg1Of bands,IκB
R4994 T5639 T5640 arg1Of bands,were
R4995 T5639 T5641 arg2Of bands,revealed
R4996 T5641 T5636 arg1Of revealed,Finally
R4997 T5641 T5637 arg1Of revealed,","
R4998 T5641 T5640 arg2Of revealed,were
R4999 T5641 T5642 modOf revealed,using
R5000 T5641 T5647 arg1Of revealed,(
R5001 T5641 T5657 arg1Of revealed,according
R5002 T5646 T5642 arg2Of system,using
R5003 T5646 T5643 arg1Of system,the
R5004 T5646 T5644 arg1Of system,ECL™
R5005 T5646 T5645 arg1Of system,detection
R5006 T5650 T5647 arg2Of Biotech,(
R5007 T5650 T5648 arg1Of Biotech,Amersham
R5008 T5650 T5649 arg1Of Biotech,Pharmacia
R5009 T5650 T5651 arg1Of Biotech,","
R5010 T5653 T5651 arg2Of Ullis,","
R5011 T5653 T5652 arg1Of Ullis,Les
R5012 T5653 T5654 arg1Of Ullis,","
R5013 T5655 T5654 arg2Of France,","
R5014 T5656 T5647 arg3Of ),(
R5015 T5658 T5657 arg2Of to,according
R5016 T5660 T5659 arg1Of manufacturers,the
R5017 T5660 T5661 arg2Of manufacturers,'
R5018 T5662 T5658 arg2Of instruction,to
R5019 T5662 T5661 arg1Of instruction,'
R5020 T5663 T5664 arg1Of Antibody,to
R5021 T5663 T5670 arg1Of Antibody,was
R5022 T5665 T5664 arg2Of α-Tubulin,to
R5023 T5665 T5666 arg1Of α-Tubulin,(
R5024 T5668 T5666 arg2Of Cruz,(
R5025 T5668 T5667 arg1Of Cruz,Santa
R5026 T5669 T5666 arg3Of ),(
R5027 T5670 T5672 arg1Of was,as
R5028 T5671 T5670 arg2Of use,was
R5029 T5673 T5672 arg2Of loading,as
R5030 T5674 T5673 arg2Of control,loading
R5031 T5677 T5675 arg2Of NF-κB,For
R5032 T5677 T5676 arg1Of NF-κB,nuclear
R5033 T5680 T5679 arg1Of cells,THP-1
R5034 T5680 T5681 arg1Of cells,were
R5035 T5680 T5682 arg2Of cells,stimulated
R5036 T5682 T5681 arg2Of stimulated,were
R5037 T5682 T5683 arg1Of stimulated,with
R5038 T5686 T5687 arg1Of C10,or
R5039 T5687 T5683 arg2Of or,with
R5040 T5687 T5684 arg1Of or,1
R5041 T5687 T5685 arg1Of or,mg/ml
R5042 T5687 T5689 arg1Of or,for
R5043 T5688 T5687 arg2Of C40,or
R5044 T5691 T5690 arg1Of minutes,30
R5045 T5691 T5692 arg1Of minutes,at
R5046 T5691 T5695 arg1Of minutes,were
R5047 T5691 T5697 arg2Of minutes,pelleted
R5048 T5694 T5692 arg2Of Cells,at
R5049 T5694 T5693 arg1Of Cells,37°C.
R5050 T5697 T5675 arg1Of pelleted,For
R5051 T5697 T5678 arg1Of pelleted,","
R5052 T5697 T5681 modOf pelleted,were
R5053 T5697 T5695 arg2Of pelleted,were
R5054 T5697 T5696 arg1Of pelleted,then
R5055 T5697 T5698 arg1Of pelleted,and
R5056 T5699 T5697 arg3Of nuclei,pelleted
R5057 T5699 T5700 arg2Of nuclei,separated
R5058 T5700 T5701 arg1Of separated,as
R5059 T5702 T5701 arg2Of described,as
R5060 T5702 T5703 arg1Of described,[
R5061 T5704 T5703 arg2Of 31,[
R5062 T5705 T5703 arg3Of ],[
R5063 T5706 T5707 arg1Of Nuclei,were
R5064 T5706 T5708 arg2Of Nuclei,washed
R5065 T5706 T5710 arg2Of Nuclei,homogenized
R5066 T5706 T5719 arg2Of Nuclei,heated
R5067 T5708 T5709 arg1Of washed,and
R5068 T5709 T5707 arg2Of and,were
R5069 T5709 T5726 arg1Of and,in
R5070 T5710 T5711 arg1Of homogenized,directly
R5071 T5710 T5712 arg1Of homogenized,in
R5072 T5710 T5718 arg1Of homogenized,and
R5073 T5715 T5714 arg2Of Laemli,(
R5074 T5716 T5714 arg3Of ),(
R5075 T5717 T5712 arg2Of buffer,in
R5076 T5717 T5713 arg1Of buffer,loading
R5077 T5717 T5714 arg1Of buffer,(
R5078 T5718 T5709 arg2Of and,and
R5079 T5719 T5718 arg2Of heated,and
R5080 T5719 T5720 arg1Of heated,for
R5081 T5722 T5720 arg2Of minutes,for
R5082 T5722 T5721 arg1Of minutes,5
R5083 T5722 T5723 arg1Of minutes,at
R5084 T5725 T5723 arg2Of Proteins,at
R5085 T5725 T5724 arg1Of Proteins,100°C.
R5086 T5727 T5728 arg1Of samples,were
R5087 T5727 T5729 arg2Of samples,resolved
R5088 T5727 T5738 arg2Of samples,transferred
R5089 T5729 T5730 arg1Of resolved,in
R5090 T5729 T5737 arg1Of resolved,and
R5091 T5733 T5734 arg1Of 8,%
R5092 T5736 T5730 arg2Of gel,in
R5093 T5736 T5731 arg1Of gel,a
R5094 T5736 T5732 arg1Of gel,denaturing
R5095 T5736 T5733 arg1Of gel,8
R5096 T5736 T5735 arg1Of gel,polyacrylamide
R5097 T5737 T5726 arg2Of and,in
R5098 T5737 T5728 arg2Of and,were
R5099 T5738 T5737 arg2Of transferred,and
R5100 T5738 T5739 arg1Of transferred,to
R5101 T5744 T5743 arg2Of PVDF,(
R5102 T5745 T5743 arg3Of ),(
R5103 T5746 T5739 arg2Of membrane,to
R5104 T5746 T5740 arg1Of membrane,a
R5105 T5746 T5741 arg1Of membrane,polyvinylidine
R5106 T5746 T5742 arg1Of membrane,fluoride
R5107 T5746 T5743 arg1Of membrane,(
R5108 T5746 T5747 arg1Of membrane,(
R5109 T5748 T5747 arg2Of Immobilon-P,(
R5110 T5748 T5749 arg1Of Immobilon-P,;
R5111 T5748 T5753 arg1Of Immobilon-P,","
R5112 T5748 T5754 arg1Of Immobilon-P,MA
R5113 T5750 T5751 arg1Of Millipore,","
R5114 T5751 T5749 arg2Of ",",;
R5115 T5752 T5751 arg2Of Bedford,","
R5116 T5755 T5747 arg3Of ),(
R5117 T5756 T5757 arg1Of Membranes,were
R5118 T5756 T5758 arg2Of Membranes,incubated
R5119 T5758 T5757 arg2Of incubated,were
R5120 T5758 T5759 arg1Of incubated,in
R5121 T5760 T5759 arg2Of blocking,in
R5122 T5761 T5760 arg2Of buffer,blocking
R5123 T5761 T5762 arg1Of buffer,(
R5124 T5761 T5770 arg1Of buffer,for
R5125 T5763 T5764 arg1Of 1,%
R5126 T5765 T5762 arg2Of BSA,(
R5127 T5765 T5763 arg1Of BSA,1
R5128 T5765 T5766 arg1Of BSA,","
R5129 T5765 T5767 arg1Of BSA,in
R5130 T5768 T5767 arg2Of PBS,in
R5131 T5769 T5762 arg3Of ),(
R5132 T5772 T5770 arg2Of hours,for
R5133 T5772 T5771 arg1Of hours,two
R5134 T5772 T5773 arg1Of hours,at
R5135 T5775 T5773 arg2Of temperature,at
R5136 T5775 T5774 arg1Of temperature,room
R5137 T5776 T5777 arg1Of Membranes,were
R5138 T5776 T5779 arg2Of Membranes,probed
R5139 T5779 T5777 arg2Of probed,were
R5140 T5779 T5778 arg1Of probed,subsequently
R5141 T5779 T5780 arg1Of probed,with
R5142 T5779 T5787 arg1Of probed,","
R5143 T5779 T5788 arg1Of probed,overnight
R5144 T5783 T5780 arg2Of antibody,with
R5145 T5783 T5781 arg1Of antibody,the
R5146 T5783 T5782 arg1Of antibody,corresponding
R5147 T5783 T5784 arg1Of antibody,in
R5148 T5785 T5784 arg2Of blocking,in
R5149 T5786 T5785 arg2Of buffer,blocking
R5150 T5794 T5789 arg1Of subunit,Rabbit
R5151 T5794 T5790 arg1Of subunit,polyclonal
R5152 T5794 T5791 arg1Of subunit,antibody
R5153 T5794 T5792 arg1Of subunit,anti-NF-κB
R5154 T5794 T5793 arg1Of subunit,p50
R5155 T5794 T5795 arg1Of subunit,(
R5156 T5794 T5799 arg1Of subunit,or
R5157 T5797 T5795 arg2Of sc-114,(
R5158 T5797 T5796 arg1Of sc-114,#
R5159 T5798 T5795 arg3Of ),(
R5160 T5799 T5807 arg1Of or,from
R5161 T5799 T5811 arg1Of or,were
R5162 T5799 T5812 arg2Of or,used
R5163 T5802 T5799 arg2Of subunit,or
R5164 T5802 T5800 arg1Of subunit,anti-NF-κB
R5165 T5802 T5801 arg1Of subunit,p65
R5166 T5802 T5803 arg1Of subunit,(
R5167 T5805 T5803 arg2Of sc-109,(
R5168 T5805 T5804 arg1Of sc-109,#
R5169 T5806 T5803 arg3Of ),(
R5170 T5810 T5807 arg2Of Biotechnology,from
R5171 T5810 T5808 arg1Of Biotechnology,Santa
R5172 T5810 T5809 arg1Of Biotechnology,Cruz
R5173 T5812 T5811 arg2Of used,were
R5174 T5813 T5814 arg1Of Membranes,were
R5175 T5813 T5815 arg2Of Membranes,washed
R5176 T5813 T5832 arg2Of Membranes,incubated
R5177 T5815 T5817 arg1Of washed,times
R5178 T5815 T5818 arg1Of washed,in
R5179 T5815 T5820 arg1Of washed,with
R5180 T5815 T5824 arg1Of washed,20
R5181 T5815 T5827 arg1Of washed,minutes
R5182 T5815 T5829 arg1Of washed,time
R5183 T5815 T5831 arg1Of washed,and
R5184 T5817 T5816 arg1Of times,six
R5185 T5819 T5818 arg2Of PBS,in
R5186 T5822 T5820 arg2Of %,with
R5187 T5822 T5821 arg1Of %,0.05
R5188 T5824 T5823 arg1Of 20,Tween
R5189 T5824 T5825 arg1Of 20,","
R5190 T5827 T5825 arg2Of minutes,","
R5191 T5827 T5826 arg1Of minutes,5
R5192 T5829 T5828 arg1Of time,each
R5193 T5831 T5814 arg2Of and,were
R5194 T5831 T5830 arg1Of and,","
R5195 T5832 T5831 arg2Of incubated,and
R5196 T5832 T5833 arg1Of incubated,with
R5197 T5832 T5861 arg1Of incubated,for
R5198 T5833 T5859 arg1Of with,in
R5199 T5836 T5834 arg1Of dilution,a
R5200 T5836 T5835 arg1Of dilution,1/3000
R5201 T5836 T5837 arg1Of dilution,of
R5202 T5839 T5837 arg2Of F,of
R5203 T5839 T5838 arg1Of F,HRP-conjugated
R5204 T5841 T5840 arg2Of ab,(
R5205 T5841 T5842 arg1Of ab,'
R5206 T5843 T5840 arg3Of ),(
R5207 T5847 T5833 arg2Of IgG,with
R5208 T5847 T5836 arg1Of IgG,dilution
R5209 T5847 T5840 arg1Of IgG,(
R5210 T5847 T5844 arg1Of IgG,2
R5211 T5847 T5845 arg1Of IgG,goat
R5212 T5847 T5846 arg1Of IgG,anti-rabbit
R5213 T5847 T5848 arg1Of IgG,in
R5214 T5849 T5850 arg1Of 5,%
R5215 T5853 T5849 arg1Of milk,5
R5216 T5853 T5851 arg1Of milk,nonfat
R5217 T5853 T5852 arg1Of milk,dry
R5218 T5853 T5854 arg1Of milk,and
R5219 T5854 T5848 arg2Of and,in
R5220 T5856 T5855 arg1Of %,0.05
R5221 T5858 T5854 arg2Of 20,and
R5222 T5858 T5856 arg1Of 20,%
R5223 T5858 T5857 arg1Of 20,Tween
R5224 T5860 T5859 arg2Of PBS,in
R5225 T5863 T5861 arg2Of hour,for
R5226 T5863 T5862 arg1Of hour,1
R5227 T5863 T5864 arg1Of hour,at
R5228 T5866 T5864 arg2Of temperature,at
R5229 T5866 T5865 arg1Of temperature,room
R5230 T5868 T5867 arg2Of washing,After
R5231 T5868 T5872 arg1Of washing,in
R5232 T5868 T5874 arg1Of washing,with
R5233 T5870 T5869 arg1Of more,six
R5234 T5871 T5868 arg2Of times,washing
R5235 T5871 T5870 arg1Of times,more
R5236 T5873 T5872 arg2Of PBS,in
R5237 T5876 T5875 arg1Of %,0.05
R5238 T5878 T5874 arg2Of 20,with
R5239 T5878 T5876 arg1Of 20,%
R5240 T5878 T5877 arg1Of 20,Tween
R5241 T5881 T5868 arg1Of proteins,washing
R5242 T5881 T5880 arg1Of proteins,antibody-reactive
R5243 T5881 T5882 arg1Of proteins,were
R5244 T5881 T5883 arg2Of proteins,detected
R5245 T5883 T5867 arg1Of detected,After
R5246 T5883 T5879 arg1Of detected,","
R5247 T5883 T5882 arg2Of detected,were
R5248 T5884 T5883 arg3Of using,detected
R5249 T5884 T5888 arg1Of using,(
R5250 T5884 T5897 arg1Of using,according
R5251 T5887 T5884 arg2Of substrate,using
R5252 T5887 T5885 arg1Of substrate,a
R5253 T5887 T5886 arg1Of substrate,chemiluminescence
R5254 T5889 T5890 arg1Of SuperSignal,;
R5255 T5890 T5888 arg2Of ;,(
R5256 T5890 T5894 arg1Of ;,","
R5257 T5891 T5892 arg1Of Pierce,","
R5258 T5892 T5890 arg2Of ",",;
R5259 T5893 T5892 arg2Of Rockford,","
R5260 T5895 T5894 arg2Of IL,","
R5261 T5896 T5888 arg3Of ),(
R5262 T5898 T5897 arg2Of to,according
R5263 T5900 T5899 arg1Of manufacturer,the
R5264 T5900 T5901 arg2Of manufacturer,'s
R5265 T5902 T5898 arg2Of instructions,to
R5266 T5902 T5901 arg1Of instructions,'s
R5267 T5904 T5903 arg1Of confirm,To
R5268 T5907 T5906 arg1Of amounts,equivalent
R5269 T5907 T5908 arg1Of amounts,of
R5270 T5907 T5910 arg1Of amounts,were
R5271 T5907 T5911 arg2Of amounts,loaded
R5272 T5909 T5908 arg2Of protein,of
R5273 T5911 T5904 arg2Of loaded,confirm
R5274 T5911 T5905 arg1Of loaded,that
R5275 T5911 T5910 arg2Of loaded,were
R5276 T5911 T5912 arg1Of loaded,in
R5277 T5914 T5912 arg2Of line,in
R5278 T5914 T5913 arg1Of line,each
R5279 T5916 T5904 arg1Of membranes,confirm
R5280 T5916 T5917 arg1Of membranes,were
R5281 T5916 T5920 arg1Of membranes,blotted
R5282 T5917 T5903 modOf were,To
R5283 T5917 T5915 arg1Of were,","
R5284 T5917 T5918 arg1Of were,also
R5285 T5917 T5923 arg1Of were,as
R5286 T5920 T5917 arg2Of blotted,were
R5287 T5920 T5919 arg1Of blotted,Western
R5288 T5920 T5921 arg1Of blotted,for
R5289 T5922 T5921 arg2Of ERK,for
R5290 T5924 T5923 arg2Of described,as
R5291 T5924 T5925 arg1Of described,[
R5292 T5926 T5925 arg2Of 32,[
R5293 T5927 T5925 arg3Of ],[

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T5933 0-7 JJ denotes Western
T5934 8-12 NNP denotes Blot
T5935 13-21 NNP denotes Analysis
T5936 22-27 NNP denotes THP-1
T5937 28-33 NNS denotes cells
T5938 34-38 VBD denotes were
T5939 39-49 VBN denotes stimulated
T5940 50-53 IN denotes for
T5941 54-61 JJ denotes various
T5942 62-69 NNS denotes lengths
T5943 70-72 IN denotes of
T5944 73-77 NN denotes time
T5945 78-82 IN denotes with
T5946 83-86 CD denotes 0.1
T5947 87-92 NN denotes mg/ml
T5948 93-96 CD denotes C10
T5949 97-99 CC denotes or
T5950 100-103 CD denotes C40
T5951 103-104 , denotes ,
T5952 105-107 CC denotes or
T5953 108-110 CD denotes 10
T5954 111-113 NN denotes µg
T5955 113-114 NN denotes /
T5956 114-116 NN denotes ml
T5957 117-120 NNP denotes LPS
T5958 120-121 . denotes .
T5959 122-127 NNS denotes Cells
T5960 128-132 VBD denotes were
T5961 133-137 RB denotes then
T5962 138-146 VBN denotes pelleted
T5963 146-147 , denotes ,
T5964 148-154 VBN denotes washed
T5965 155-158 CC denotes and
T5966 159-170 VBN denotes homogenised
T5967 171-173 IN denotes in
T5968 174-179 NN denotes lysis
T5969 180-186 NN denotes buffer
T5970 187-188 -LRB- denotes (
T5971 188-190 CD denotes 10
T5972 191-193 NNP denotes mM
T5973 194-199 NNP denotes Hepes
T5974 199-200 , denotes ,
T5975 201-203 NNP denotes pH
T5976 204-207 CD denotes 7.9
T5977 207-208 , denotes ,
T5978 209-212 CD denotes 150
T5979 213-215 NNP denotes mM
T5980 216-220 NNP denotes NaCl
T5981 220-221 , denotes ,
T5982 222-223 CD denotes 1
T5983 224-226 NNP denotes mM
T5984 227-231 NNP denotes EDTA
T5985 231-232 , denotes ,
T5986 233-236 CD denotes 0.6
T5987 236-237 NN denotes %
T5988 238-243 NN denotes NP-40
T5989 243-244 , denotes ,
T5990 245-248 CC denotes and
T5991 249-252 CD denotes 0.5
T5992 253-255 NNP denotes mM
T5993 256-260 NNP denotes PMSF
T5994 260-261 -RRB- denotes )
T5995 262-264 IN denotes on
T5996 265-268 NN denotes ice
T5997 268-269 . denotes .
T5998 270-281 NNS denotes Homogenates
T5999 282-286 VBD denotes were
T6000 287-296 VBN denotes sonicated
T6001 296-297 , denotes ,
T6002 298-309 VBN denotes centrifuged
T6003 310-312 IN denotes at
T6004 313-319 CD denotes 10,000
T6005 320-323 NN denotes rpm
T6006 324-326 TO denotes to
T6007 327-333 VB denotes remove
T6008 334-342 JJ denotes cellular
T6009 343-349 NN denotes debris
T6010 349-350 , denotes ,
T6011 351-354 CC denotes and
T6012 355-366 JJ denotes supernatant
T6013 367-376 VBN denotes collected
T6014 376-377 . denotes .
T6015 378-385 NN denotes Protein
T6016 386-399 NN denotes concentration
T6017 400-403 VBD denotes was
T6018 404-414 VBN denotes determined
T6019 415-420 VBG denotes using
T6020 421-424 DT denotes the
T6021 425-427 NNP denotes DC
T6022 428-435 NNP denotes Protein
T6023 436-441 NNP denotes Assay
T6024 442-443 -LRB- denotes (
T6025 443-450 NNP denotes Bio-Rad
T6026 450-451 -RRB- denotes )
T6027 451-452 . denotes .
T6028 453-461 NNPS denotes Proteins
T6029 462-464 IN denotes in
T6030 465-472 NNS denotes samples
T6031 473-474 -LRB- denotes (
T6032 474-476 CD denotes 15
T6033 477-479 NN denotes µg
T6034 480-485 JJ denotes total
T6035 486-494 NNS denotes proteins
T6036 494-495 -RRB- denotes )
T6037 496-500 VBD denotes were
T6038 501-509 VBN denotes resolved
T6039 510-512 IN denotes in
T6040 513-514 DT denotes a
T6041 515-525 VBG denotes denaturing
T6042 526-528 CD denotes 12
T6043 528-529 NN denotes %
T6044 530-544 NN denotes polyacrylamide
T6045 545-548 NN denotes gel
T6046 549-552 CC denotes and
T6047 553-564 VBN denotes transferred
T6048 565-567 TO denotes to
T6049 568-569 DT denotes a
T6050 570-584 JJ denotes nitrocellulose
T6051 585-593 NN denotes membrane
T6052 593-594 . denotes .
T6053 595-600 JJ denotes I-κBα
T6054 601-608 NN denotes protein
T6055 609-612 VBD denotes was
T6056 613-621 VBN denotes detected
T6057 622-627 VBG denotes using
T6058 628-629 DT denotes a
T6059 630-636 NN denotes rabbit
T6060 637-647 NN denotes polyclonal
T6061 648-656 NN denotes antibody
T6062 657-658 -LRB- denotes (
T6063 658-663 NNP denotes Santa
T6064 664-668 NNP denotes Cruz
T6065 669-682 NNP denotes Biotechnology
T6066 682-683 , denotes ,
T6067 684-686 NNP denotes CA
T6068 686-687 -RRB- denotes )
T6069 688-696 VBN denotes followed
T6070 697-699 IN denotes by
T6071 700-701 DT denotes a
T6072 702-713 JJ denotes horseradish
T6073 714-732 JJ denotes peroxidase-coupled
T6074 733-737 NN denotes goat
T6075 738-748 NN denotes polyclonal
T6076 749-757 NN denotes antibody
T6077 758-765 IN denotes against
T6078 766-772 NN denotes rabbit
T6079 773-775 NNP denotes Ig
T6080 776-777 -LRB- denotes (
T6081 777-783 NNP denotes Caltag
T6082 784-796 NNPS denotes Laboratories
T6083 796-797 -RRB- denotes )
T6084 797-798 . denotes .
T6085 799-806 RB denotes Finally
T6086 806-807 , denotes ,
T6087 808-811 NNP denotes IκB
T6088 812-817 NNS denotes bands
T6089 818-822 VBD denotes were
T6090 823-831 VBN denotes revealed
T6091 832-837 VBG denotes using
T6092 838-841 DT denotes the
T6093 842-845 NNP denotes ECL
T6094 845-846 NN denotes ™
T6095 847-856 NN denotes detection
T6096 857-863 NN denotes system
T6097 864-865 -LRB- denotes (
T6098 865-873 NNP denotes Amersham
T6099 874-883 NNP denotes Pharmacia
T6100 884-891 NNP denotes Biotech
T6101 891-892 , denotes ,
T6102 893-896 NNP denotes Les
T6103 897-902 NNP denotes Ullis
T6104 902-903 , denotes ,
T6105 904-910 NNP denotes France
T6106 910-911 -RRB- denotes )
T6107 912-921 VBG denotes according
T6108 922-924 TO denotes to
T6109 925-928 DT denotes the
T6110 929-942 NNS denotes manufacturers
T6111 942-943 POS denotes '
T6112 944-955 NN denotes instruction
T6113 955-956 . denotes .
T6114 957-965 NN denotes Antibody
T6115 966-968 TO denotes to
T6116 969-978 NNP denotes α-Tubulin
T6117 979-980 -LRB- denotes (
T6118 980-985 NNP denotes Santa
T6119 986-990 NNP denotes Cruz
T6120 990-991 -RRB- denotes )
T6121 992-995 VBD denotes was
T6122 996-999 NN denotes use
T6123 1000-1002 IN denotes as
T6124 1003-1010 VBG denotes loading
T6125 1011-1018 NN denotes control
T6126 1018-1019 . denotes .
T6127 1020-1023 IN denotes For
T6128 1024-1031 JJ denotes nuclear
T6129 1032-1037 NNP denotes NF-κB
T6130 1037-1038 , denotes ,
T6131 1039-1044 NNP denotes THP-1
T6132 1045-1050 NNS denotes cells
T6133 1051-1055 VBD denotes were
T6134 1056-1066 VBN denotes stimulated
T6135 1067-1071 IN denotes with
T6136 1072-1073 CD denotes 1
T6137 1074-1079 NN denotes mg/ml
T6138 1080-1083 CD denotes C10
T6139 1084-1086 CC denotes or
T6140 1087-1090 CD denotes C40
T6141 1091-1094 IN denotes for
T6142 1095-1097 CD denotes 30
T6143 1098-1105 NNS denotes minutes
T6144 1106-1108 IN denotes at
T6145 1109-1111 CD denotes 37
T6146 1111-1112 NN denotes °
T6147 1112-1114 NNP denotes C.
T6148 1115-1120 NNP denotes Cells
T6149 1121-1125 VBD denotes were
T6150 1126-1130 RB denotes then
T6151 1131-1139 VBN denotes pelleted
T6152 1140-1143 CC denotes and
T6153 1144-1150 JJ denotes nuclei
T6154 1151-1160 VBN denotes separated
T6155 1161-1163 IN denotes as
T6156 1164-1173 VBN denotes described
T6157 1174-1175 NNP denotes [
T6158 1175-1177 CD denotes 31
T6159 1177-1178 NNP denotes ]
T6160 1178-1179 . denotes .
T6161 1180-1186 NNP denotes Nuclei
T6162 1187-1191 VBD denotes were
T6163 1192-1198 VBN denotes washed
T6164 1199-1202 CC denotes and
T6165 1203-1214 VBN denotes homogenized
T6166 1215-1223 RB denotes directly
T6167 1224-1226 IN denotes in
T6168 1227-1234 VBG denotes loading
T6169 1235-1236 -LRB- denotes (
T6170 1236-1242 NNP denotes Laemli
T6171 1242-1243 -RRB- denotes )
T6172 1244-1250 NN denotes buffer
T6173 1251-1254 CC denotes and
T6174 1255-1261 VBN denotes heated
T6175 1262-1265 IN denotes for
T6176 1266-1267 CD denotes 5
T6177 1268-1275 NNS denotes minutes
T6178 1276-1278 IN denotes at
T6179 1279-1282 CD denotes 100
T6180 1282-1283 CD denotes °
T6181 1283-1285 NNP denotes C.
T6182 1286-1294 NNPS denotes Proteins
T6183 1295-1297 IN denotes in
T6184 1298-1305 NNS denotes samples
T6185 1306-1310 VBD denotes were
T6186 1311-1319 VBN denotes resolved
T6187 1320-1322 IN denotes in
T6188 1323-1324 DT denotes a
T6189 1325-1335 NN denotes denaturing
T6190 1336-1337 CD denotes 8
T6191 1337-1338 NN denotes %
T6192 1339-1353 NN denotes polyacrylamide
T6193 1354-1357 NN denotes gel
T6194 1358-1361 CC denotes and
T6195 1362-1373 VBN denotes transferred
T6196 1374-1376 TO denotes to
T6197 1377-1378 DT denotes a
T6198 1379-1393 NN denotes polyvinylidine
T6199 1394-1402 NN denotes fluoride
T6200 1403-1404 -LRB- denotes (
T6201 1404-1408 NNP denotes PVDF
T6202 1408-1409 -RRB- denotes )
T6203 1410-1418 NN denotes membrane
T6204 1419-1420 -LRB- denotes (
T6205 1420-1431 JJ denotes Immobilon-P
T6206 1431-1432 : denotes ;
T6207 1433-1442 NNP denotes Millipore
T6208 1442-1443 , denotes ,
T6209 1444-1451 NNP denotes Bedford
T6210 1451-1452 , denotes ,
T6211 1453-1455 NNP denotes MA
T6212 1455-1456 -RRB- denotes )
T6213 1456-1457 . denotes .
T6214 1458-1467 NNS denotes Membranes
T6215 1468-1472 VBD denotes were
T6216 1473-1482 VBN denotes incubated
T6217 1483-1485 IN denotes in
T6218 1486-1494 VBG denotes blocking
T6219 1495-1501 NN denotes buffer
T6220 1502-1503 -LRB- denotes (
T6221 1503-1504 CD denotes 1
T6222 1504-1505 NN denotes %
T6223 1506-1509 NNP denotes BSA
T6224 1509-1510 , denotes ,
T6225 1511-1513 IN denotes in
T6226 1514-1517 NNP denotes PBS
T6227 1517-1518 -RRB- denotes )
T6228 1519-1522 IN denotes for
T6229 1523-1526 CD denotes two
T6230 1527-1532 NNS denotes hours
T6231 1533-1535 IN denotes at
T6232 1536-1540 NN denotes room
T6233 1541-1552 NN denotes temperature
T6234 1552-1553 . denotes .
T6235 1554-1563 NNS denotes Membranes
T6236 1564-1568 VBD denotes were
T6237 1569-1581 RB denotes subsequently
T6238 1582-1588 VBN denotes probed
T6239 1589-1593 IN denotes with
T6240 1594-1597 DT denotes the
T6241 1598-1611 JJ denotes corresponding
T6242 1612-1620 NN denotes antibody
T6243 1621-1623 IN denotes in
T6244 1624-1632 VBG denotes blocking
T6245 1633-1639 NN denotes buffer
T6246 1639-1640 , denotes ,
T6247 1641-1650 JJ denotes overnight
T6248 1650-1651 . denotes .
T6249 1652-1658 NN denotes Rabbit
T6250 1659-1669 NN denotes polyclonal
T6251 1670-1678 NN denotes antibody
T6252 1679-1689 JJ denotes anti-NF-κB
T6253 1690-1693 JJ denotes p50
T6254 1694-1701 NN denotes subunit
T6255 1702-1703 -LRB- denotes (
T6256 1703-1704 # denotes #
T6257 1705-1711 CD denotes sc-114
T6258 1711-1712 -RRB- denotes )
T6259 1713-1715 CC denotes or
T6260 1716-1726 JJ denotes anti-NF-κB
T6261 1727-1730 NNS denotes p65
T6262 1731-1738 VBN denotes subunit
T6263 1739-1740 -LRB- denotes (
T6264 1740-1741 # denotes #
T6265 1742-1748 CD denotes sc-109
T6266 1748-1749 -RRB- denotes )
T6267 1750-1754 IN denotes from
T6268 1755-1760 NNP denotes Santa
T6269 1761-1765 NNP denotes Cruz
T6270 1766-1779 NNP denotes Biotechnology
T6271 1780-1784 VBD denotes were
T6272 1785-1789 VBN denotes used
T6273 1789-1790 . denotes .
T6274 1791-1800 NNS denotes Membranes
T6275 1801-1805 VBD denotes were
T6276 1806-1812 VBN denotes washed
T6277 1813-1816 CD denotes six
T6278 1817-1822 NNS denotes times
T6279 1823-1825 IN denotes in
T6280 1826-1829 NNP denotes PBS
T6281 1830-1834 IN denotes with
T6282 1835-1839 CD denotes 0.05
T6283 1839-1840 NN denotes %
T6284 1841-1846 CD denotes Tween
T6285 1847-1849 CD denotes 20
T6286 1849-1850 , denotes ,
T6287 1851-1852 CD denotes 5
T6288 1853-1860 NNS denotes minutes
T6289 1861-1865 DT denotes each
T6290 1866-1870 NN denotes time
T6291 1870-1871 , denotes ,
T6292 1872-1875 CC denotes and
T6293 1876-1885 VBN denotes incubated
T6294 1886-1890 IN denotes with
T6295 1891-1892 DT denotes a
T6296 1893-1899 CD denotes 1/3000
T6297 1900-1908 NN denotes dilution
T6298 1909-1911 IN denotes of
T6299 1912-1926 JJ denotes HRP-conjugated
T6300 1927-1928 NN denotes F
T6301 1928-1929 -LRB- denotes (
T6302 1929-1931 NN denotes ab
T6303 1931-1932 '' denotes '
T6304 1932-1933 -RRB- denotes )
T6305 1933-1934 CD denotes 2
T6306 1935-1939 NN denotes goat
T6307 1940-1951 JJ denotes anti-rabbit
T6308 1952-1955 NNP denotes IgG
T6309 1956-1958 IN denotes in
T6310 1959-1960 CD denotes 5
T6311 1960-1961 NN denotes %
T6312 1962-1968 JJ denotes nonfat
T6313 1969-1972 JJ denotes dry
T6314 1973-1977 NN denotes milk
T6315 1978-1981 CC denotes and
T6316 1982-1986 CD denotes 0.05
T6317 1986-1987 NN denotes %
T6318 1988-1993 CD denotes Tween
T6319 1994-1996 CD denotes 20
T6320 1997-1999 IN denotes in
T6321 2000-2003 NNP denotes PBS
T6322 2004-2007 IN denotes for
T6323 2008-2009 CD denotes 1
T6324 2010-2014 NN denotes hour
T6325 2015-2017 IN denotes at
T6326 2018-2022 NN denotes room
T6327 2023-2034 NN denotes temperature
T6328 2034-2035 . denotes .
T6329 2036-2041 IN denotes After
T6330 2042-2049 VBG denotes washing
T6331 2050-2053 CD denotes six
T6332 2054-2058 JJR denotes more
T6333 2059-2064 NNS denotes times
T6334 2065-2067 IN denotes in
T6335 2068-2071 NNP denotes PBS
T6336 2072-2076 IN denotes with
T6337 2077-2081 CD denotes 0.05
T6338 2081-2082 NN denotes %
T6339 2083-2088 CD denotes Tween
T6340 2089-2091 CD denotes 20
T6341 2091-2092 , denotes ,
T6342 2093-2110 JJ denotes antibody-reactive
T6343 2111-2119 NNS denotes proteins
T6344 2120-2124 VBD denotes were
T6345 2125-2133 VBN denotes detected
T6346 2134-2139 VBG denotes using
T6347 2140-2141 DT denotes a
T6348 2142-2159 NN denotes chemiluminescence
T6349 2160-2169 NN denotes substrate
T6350 2170-2171 -LRB- denotes (
T6351 2171-2182 NNP denotes SuperSignal
T6352 2182-2183 : denotes ;
T6353 2184-2190 NNP denotes Pierce
T6354 2190-2191 , denotes ,
T6355 2192-2200 NNP denotes Rockford
T6356 2200-2201 , denotes ,
T6357 2202-2204 NNP denotes IL
T6358 2204-2205 -RRB- denotes )
T6359 2206-2215 VBG denotes according
T6360 2216-2218 TO denotes to
T6361 2219-2222 DT denotes the
T6362 2223-2235 NN denotes manufacturer
T6363 2235-2237 POS denotes 's
T6364 2238-2250 NNS denotes instructions
T6365 2250-2251 . denotes .
T6366 2252-2254 TO denotes To
T6367 2255-2262 VB denotes confirm
T6368 2263-2267 DT denotes that
T6369 2268-2278 JJ denotes equivalent
T6370 2279-2286 NNS denotes amounts
T6371 2287-2289 IN denotes of
T6372 2290-2297 NN denotes protein
T6373 2298-2302 VBD denotes were
T6374 2303-2309 VBN denotes loaded
T6375 2310-2312 IN denotes in
T6376 2313-2317 DT denotes each
T6377 2318-2322 NN denotes line
T6378 2322-2323 , denotes ,
T6379 2324-2333 NNS denotes membranes
T6380 2334-2338 VBD denotes were
T6381 2339-2343 RB denotes also
T6382 2344-2351 JJ denotes Western
T6383 2352-2359 VBN denotes blotted
T6384 2360-2363 IN denotes for
T6385 2364-2367 NNP denotes ERK
T6386 2368-2370 IN denotes as
T6387 2371-2380 VBN denotes described
T6388 2381-2382 NNP denotes [
T6389 2382-2384 CD denotes 32
T6390 2384-2385 NNP denotes ]
T6391 2385-2386 . denotes .
R5294 T5933 T5937 amod Western,cells
R5295 T5934 T5936 compound Blot,THP-1
R5296 T5935 T5936 compound Analysis,THP-1
R5297 T5936 T5937 compound THP-1,cells
R5298 T5937 T5939 nsubjpass cells,stimulated
R5299 T5938 T5939 auxpass were,stimulated
R5300 T5939 T5939 ROOT stimulated,stimulated
R5301 T5940 T5939 prep for,stimulated
R5302 T5941 T5942 amod various,lengths
R5303 T5942 T5940 pobj lengths,for
R5304 T5943 T5942 prep of,lengths
R5305 T5944 T5943 pobj time,of
R5306 T5945 T5942 prep with,lengths
R5307 T5946 T5947 nummod 0.1,mg/ml
R5308 T5947 T5945 pobj mg/ml,with
R5309 T5948 T5947 nummod C10,mg/ml
R5310 T5949 T5948 cc or,C10
R5311 T5950 T5948 conj C40,C10
R5312 T5951 T5950 punct ",",C40
R5313 T5952 T5950 cc or,C40
R5314 T5953 T5954 nummod 10,µg
R5315 T5954 T5957 compound µg,LPS
R5316 T5955 T5957 compound /,LPS
R5317 T5956 T5957 compound ml,LPS
R5318 T5957 T5950 conj LPS,C40
R5319 T5958 T5939 punct .,stimulated
R5320 T5959 T5962 nsubjpass Cells,pelleted
R5321 T5960 T5962 auxpass were,pelleted
R5322 T5961 T5962 advmod then,pelleted
R5323 T5962 T5962 ROOT pelleted,pelleted
R5324 T5963 T5962 punct ",",pelleted
R5325 T5964 T5962 conj washed,pelleted
R5326 T5965 T5964 cc and,washed
R5327 T5966 T5964 conj homogenised,washed
R5328 T5967 T5966 prep in,homogenised
R5329 T5968 T5969 compound lysis,buffer
R5330 T5969 T5967 pobj buffer,in
R5331 T5970 T5973 punct (,Hepes
R5332 T5971 T5973 nummod 10,Hepes
R5333 T5972 T5973 compound mM,Hepes
R5334 T5973 T5966 appos Hepes,homogenised
R5335 T5974 T5973 punct ",",Hepes
R5336 T5975 T5973 conj pH,Hepes
R5337 T5976 T5975 nummod 7.9,pH
R5338 T5977 T5975 punct ",",pH
R5339 T5978 T5980 nummod 150,NaCl
R5340 T5979 T5980 compound mM,NaCl
R5341 T5980 T5975 conj NaCl,pH
R5342 T5981 T5980 punct ",",NaCl
R5343 T5982 T5984 nummod 1,EDTA
R5344 T5983 T5984 compound mM,EDTA
R5345 T5984 T5980 conj EDTA,NaCl
R5346 T5985 T5984 punct ",",EDTA
R5347 T5986 T5987 nummod 0.6,%
R5348 T5987 T5988 compound %,NP-40
R5349 T5988 T5984 conj NP-40,EDTA
R5350 T5989 T5988 punct ",",NP-40
R5351 T5990 T5988 cc and,NP-40
R5352 T5991 T5993 nummod 0.5,PMSF
R5353 T5992 T5993 compound mM,PMSF
R5354 T5993 T5988 conj PMSF,NP-40
R5355 T5994 T5993 punct ),PMSF
R5356 T5995 T5988 prep on,NP-40
R5357 T5996 T5995 pobj ice,on
R5358 T5997 T5962 punct .,pelleted
R5359 T5998 T6000 nsubjpass Homogenates,sonicated
R5360 T5999 T6000 auxpass were,sonicated
R5361 T6000 T6000 ROOT sonicated,sonicated
R5362 T6001 T6000 punct ",",sonicated
R5363 T6002 T6000 conj centrifuged,sonicated
R5364 T6003 T6002 prep at,centrifuged
R5365 T6004 T6005 nummod "10,000",rpm
R5366 T6005 T6003 pobj rpm,at
R5367 T6006 T6007 aux to,remove
R5368 T6007 T6002 advcl remove,centrifuged
R5369 T6008 T6009 amod cellular,debris
R5370 T6009 T6007 dobj debris,remove
R5371 T6010 T6007 punct ",",remove
R5372 T6011 T6007 cc and,remove
R5373 T6012 T6013 amod supernatant,collected
R5374 T6013 T6002 conj collected,centrifuged
R5375 T6014 T6000 punct .,sonicated
R5376 T6015 T6016 compound Protein,concentration
R5377 T6016 T6018 nsubjpass concentration,determined
R5378 T6017 T6018 auxpass was,determined
R5379 T6018 T6018 ROOT determined,determined
R5380 T6019 T6018 advcl using,determined
R5381 T6020 T6023 det the,Assay
R5382 T6021 T6023 compound DC,Assay
R5383 T6022 T6023 compound Protein,Assay
R5384 T6023 T6019 dobj Assay,using
R5385 T6024 T6023 punct (,Assay
R5386 T6025 T6023 appos Bio-Rad,Assay
R5387 T6026 T6023 punct ),Assay
R5388 T6027 T6018 punct .,determined
R5389 T6028 T6038 nsubjpass Proteins,resolved
R5390 T6029 T6028 prep in,Proteins
R5391 T6030 T6029 pobj samples,in
R5392 T6031 T6030 punct (,samples
R5393 T6032 T6033 nummod 15,µg
R5394 T6033 T6038 nsubjpass µg,resolved
R5395 T6034 T6035 amod total,proteins
R5396 T6035 T6033 appos proteins,µg
R5397 T6036 T6033 punct ),µg
R5398 T6037 T6038 auxpass were,resolved
R5399 T6038 T6038 ROOT resolved,resolved
R5400 T6039 T6038 prep in,resolved
R5401 T6040 T6045 det a,gel
R5402 T6041 T6045 amod denaturing,gel
R5403 T6042 T6043 nummod 12,%
R5404 T6043 T6045 compound %,gel
R5405 T6044 T6045 compound polyacrylamide,gel
R5406 T6045 T6039 pobj gel,in
R5407 T6046 T6038 cc and,resolved
R5408 T6047 T6038 conj transferred,resolved
R5409 T6048 T6047 prep to,transferred
R5410 T6049 T6051 det a,membrane
R5411 T6050 T6051 amod nitrocellulose,membrane
R5412 T6051 T6048 pobj membrane,to
R5413 T6052 T6038 punct .,resolved
R5414 T6053 T6054 amod I-κBα,protein
R5415 T6054 T6056 nsubjpass protein,detected
R5416 T6055 T6056 auxpass was,detected
R5417 T6056 T6056 ROOT detected,detected
R5418 T6057 T6056 advcl using,detected
R5419 T6058 T6061 det a,antibody
R5420 T6059 T6061 nmod rabbit,antibody
R5421 T6060 T6061 compound polyclonal,antibody
R5422 T6061 T6057 dobj antibody,using
R5423 T6062 T6061 punct (,antibody
R5424 T6063 T6064 compound Santa,Cruz
R5425 T6064 T6065 compound Cruz,Biotechnology
R5426 T6065 T6061 appos Biotechnology,antibody
R5427 T6066 T6065 punct ",",Biotechnology
R5428 T6067 T6065 appos CA,Biotechnology
R5429 T6068 T6061 punct ),antibody
R5430 T6069 T6056 advcl followed,detected
R5431 T6070 T6069 agent by,followed
R5432 T6071 T6076 det a,antibody
R5433 T6072 T6076 amod horseradish,antibody
R5434 T6073 T6076 amod peroxidase-coupled,antibody
R5435 T6074 T6076 compound goat,antibody
R5436 T6075 T6076 compound polyclonal,antibody
R5437 T6076 T6070 pobj antibody,by
R5438 T6077 T6076 prep against,antibody
R5439 T6078 T6079 compound rabbit,Ig
R5440 T6079 T6077 pobj Ig,against
R5441 T6080 T6082 punct (,Laboratories
R5442 T6081 T6082 compound Caltag,Laboratories
R5443 T6082 T6079 appos Laboratories,Ig
R5444 T6083 T6082 punct ),Laboratories
R5445 T6084 T6056 punct .,detected
R5446 T6085 T6090 advmod Finally,revealed
R5447 T6086 T6090 punct ",",revealed
R5448 T6087 T6088 compound IκB,bands
R5449 T6088 T6090 nsubjpass bands,revealed
R5450 T6089 T6090 auxpass were,revealed
R5451 T6090 T6090 ROOT revealed,revealed
R5452 T6091 T6090 advcl using,revealed
R5453 T6092 T6096 det the,system
R5454 T6093 T6096 nmod ECL,system
R5455 T6094 T6096 compound ™,system
R5456 T6095 T6096 compound detection,system
R5457 T6096 T6091 dobj system,using
R5458 T6097 T6096 punct (,system
R5459 T6098 T6100 compound Amersham,Biotech
R5460 T6099 T6100 compound Pharmacia,Biotech
R5461 T6100 T6096 appos Biotech,system
R5462 T6101 T6096 punct ",",system
R5463 T6102 T6103 compound Les,Ullis
R5464 T6103 T6096 appos Ullis,system
R5465 T6104 T6103 punct ",",Ullis
R5466 T6105 T6103 appos France,Ullis
R5467 T6106 T6096 punct ),system
R5468 T6107 T6090 prep according,revealed
R5469 T6108 T6107 prep to,according
R5470 T6109 T6110 det the,manufacturers
R5471 T6110 T6112 poss manufacturers,instruction
R5472 T6111 T6110 case ',manufacturers
R5473 T6112 T6108 pobj instruction,to
R5474 T6113 T6090 punct .,revealed
R5475 T6114 T6121 nsubj Antibody,was
R5476 T6115 T6114 prep to,Antibody
R5477 T6116 T6115 pobj α-Tubulin,to
R5478 T6117 T6119 punct (,Cruz
R5479 T6118 T6119 compound Santa,Cruz
R5480 T6119 T6116 appos Cruz,α-Tubulin
R5481 T6120 T6116 punct ),α-Tubulin
R5482 T6121 T6121 ROOT was,was
R5483 T6122 T6121 attr use,was
R5484 T6123 T6122 prep as,use
R5485 T6124 T6123 pcomp loading,as
R5486 T6125 T6124 dobj control,loading
R5487 T6126 T6121 punct .,was
R5488 T6127 T6134 prep For,stimulated
R5489 T6128 T6129 amod nuclear,NF-κB
R5490 T6129 T6127 pobj NF-κB,For
R5491 T6130 T6134 punct ",",stimulated
R5492 T6131 T6132 compound THP-1,cells
R5493 T6132 T6134 nsubjpass cells,stimulated
R5494 T6133 T6134 auxpass were,stimulated
R5495 T6134 T6151 ccomp stimulated,pelleted
R5496 T6135 T6134 prep with,stimulated
R5497 T6136 T6137 nummod 1,mg/ml
R5498 T6137 T6135 pobj mg/ml,with
R5499 T6138 T6137 nummod C10,mg/ml
R5500 T6139 T6138 cc or,C10
R5501 T6140 T6138 conj C40,C10
R5502 T6141 T6140 prep for,C40
R5503 T6142 T6143 nummod 30,minutes
R5504 T6143 T6141 pobj minutes,for
R5505 T6144 T6143 prep at,minutes
R5506 T6145 T6146 nummod 37,°
R5507 T6146 T6148 compound °,Cells
R5508 T6147 T6148 compound C.,Cells
R5509 T6148 T6144 pobj Cells,at
R5510 T6149 T6151 auxpass were,pelleted
R5511 T6150 T6151 advmod then,pelleted
R5512 T6151 T6151 ROOT pelleted,pelleted
R5513 T6152 T6151 cc and,pelleted
R5514 T6153 T6151 conj nuclei,pelleted
R5515 T6154 T6151 conj separated,pelleted
R5516 T6155 T6156 mark as,described
R5517 T6156 T6151 advcl described,pelleted
R5518 T6157 T6159 nmod [,]
R5519 T6158 T6159 nummod 31,]
R5520 T6159 T6156 dobj ],described
R5521 T6160 T6151 punct .,pelleted
R5522 T6161 T6163 nsubjpass Nuclei,washed
R5523 T6162 T6163 auxpass were,washed
R5524 T6163 T6163 ROOT washed,washed
R5525 T6164 T6163 cc and,washed
R5526 T6165 T6163 conj homogenized,washed
R5527 T6166 T6165 advmod directly,homogenized
R5528 T6167 T6165 prep in,homogenized
R5529 T6168 T6167 pcomp loading,in
R5530 T6169 T6172 punct (,buffer
R5531 T6170 T6172 nmod Laemli,buffer
R5532 T6171 T6172 punct ),buffer
R5533 T6172 T6168 dobj buffer,loading
R5534 T6173 T6172 cc and,buffer
R5535 T6174 T6163 conj heated,washed
R5536 T6175 T6174 prep for,heated
R5537 T6176 T6177 nummod 5,minutes
R5538 T6177 T6175 pobj minutes,for
R5539 T6178 T6174 prep at,heated
R5540 T6179 T6182 nummod 100,Proteins
R5541 T6180 T6182 nummod °,Proteins
R5542 T6181 T6182 compound C.,Proteins
R5543 T6182 T6178 pobj Proteins,at
R5544 T6183 T6182 prep in,Proteins
R5545 T6184 T6183 pobj samples,in
R5546 T6185 T6186 auxpass were,resolved
R5547 T6186 T6163 conj resolved,washed
R5548 T6187 T6186 prep in,resolved
R5549 T6188 T6193 det a,gel
R5550 T6189 T6193 amod denaturing,gel
R5551 T6190 T6191 nummod 8,%
R5552 T6191 T6193 compound %,gel
R5553 T6192 T6193 compound polyacrylamide,gel
R5554 T6193 T6187 pobj gel,in
R5555 T6194 T6186 cc and,resolved
R5556 T6195 T6186 conj transferred,resolved
R5557 T6196 T6195 prep to,transferred
R5558 T6197 T6199 det a,fluoride
R5559 T6198 T6199 compound polyvinylidine,fluoride
R5560 T6199 T6196 pobj fluoride,to
R5561 T6200 T6199 punct (,fluoride
R5562 T6201 T6199 appos PVDF,fluoride
R5563 T6202 T6199 punct ),fluoride
R5564 T6203 T6195 dobj membrane,transferred
R5565 T6204 T6205 punct (,Immobilon-P
R5566 T6205 T6203 appos Immobilon-P,membrane
R5567 T6206 T6163 punct ;,washed
R5568 T6207 T6207 ROOT Millipore,Millipore
R5569 T6208 T6207 punct ",",Millipore
R5570 T6209 T6207 conj Bedford,Millipore
R5571 T6210 T6209 punct ",",Bedford
R5572 T6211 T6209 conj MA,Bedford
R5573 T6212 T6209 punct ),Bedford
R5574 T6213 T6207 punct .,Millipore
R5575 T6214 T6216 nsubjpass Membranes,incubated
R5576 T6215 T6216 auxpass were,incubated
R5577 T6216 T6216 ROOT incubated,incubated
R5578 T6217 T6216 prep in,incubated
R5579 T6218 T6217 pcomp blocking,in
R5580 T6219 T6218 dobj buffer,blocking
R5581 T6220 T6223 punct (,BSA
R5582 T6221 T6222 nummod 1,%
R5583 T6222 T6223 npadvmod %,BSA
R5584 T6223 T6218 dobj BSA,blocking
R5585 T6224 T6218 punct ",",blocking
R5586 T6225 T6224 oprd in,","
R5587 T6226 T6225 pobj PBS,in
R5588 T6227 T6225 punct ),in
R5589 T6228 T6216 prep for,incubated
R5590 T6229 T6230 nummod two,hours
R5591 T6230 T6228 pobj hours,for
R5592 T6231 T6230 prep at,hours
R5593 T6232 T6233 compound room,temperature
R5594 T6233 T6231 pobj temperature,at
R5595 T6234 T6216 punct .,incubated
R5596 T6235 T6238 nsubjpass Membranes,probed
R5597 T6236 T6238 auxpass were,probed
R5598 T6237 T6238 advmod subsequently,probed
R5599 T6238 T6238 ROOT probed,probed
R5600 T6239 T6238 prep with,probed
R5601 T6240 T6242 det the,antibody
R5602 T6241 T6242 amod corresponding,antibody
R5603 T6242 T6239 pobj antibody,with
R5604 T6243 T6238 prep in,probed
R5605 T6244 T6243 pcomp blocking,in
R5606 T6245 T6244 dobj buffer,blocking
R5607 T6246 T6247 punct ",",overnight
R5608 T6247 T6244 npadvmod overnight,blocking
R5609 T6248 T6238 punct .,probed
R5610 T6249 T6254 nmod Rabbit,subunit
R5611 T6250 T6254 amod polyclonal,subunit
R5612 T6251 T6254 nmod antibody,subunit
R5613 T6252 T6254 nmod anti-NF-κB,subunit
R5614 T6253 T6254 amod p50,subunit
R5615 T6254 T6272 nsubjpass subunit,used
R5616 T6255 T6254 punct (,subunit
R5617 T6256 T6257 nmod #,sc-114
R5618 T6257 T6272 nsubjpass sc-114,used
R5619 T6258 T6257 punct ),sc-114
R5620 T6259 T6257 cc or,sc-114
R5621 T6260 T6261 amod anti-NF-κB,p65
R5622 T6261 T6257 conj p65,sc-114
R5623 T6262 T6261 acl subunit,p65
R5624 T6263 T6261 punct (,p65
R5625 T6264 T6265 nmod #,sc-109
R5626 T6265 T6261 appos sc-109,p65
R5627 T6266 T6261 punct ),p65
R5628 T6267 T6261 prep from,p65
R5629 T6268 T6269 compound Santa,Cruz
R5630 T6269 T6270 compound Cruz,Biotechnology
R5631 T6270 T6267 pobj Biotechnology,from
R5632 T6271 T6272 auxpass were,used
R5633 T6272 T6272 ROOT used,used
R5634 T6273 T6272 punct .,used
R5635 T6274 T6276 nsubjpass Membranes,washed
R5636 T6275 T6276 auxpass were,washed
R5637 T6276 T6276 ROOT washed,washed
R5638 T6277 T6278 nummod six,times
R5639 T6278 T6276 npadvmod times,washed
R5640 T6279 T6276 prep in,washed
R5641 T6280 T6279 pobj PBS,in
R5642 T6281 T6276 prep with,washed
R5643 T6282 T6283 nummod 0.05,%
R5644 T6283 T6288 nummod %,minutes
R5645 T6284 T6288 nummod Tween,minutes
R5646 T6285 T6288 nummod 20,minutes
R5647 T6286 T6288 punct ",",minutes
R5648 T6287 T6288 nummod 5,minutes
R5649 T6288 T6281 pobj minutes,with
R5650 T6289 T6290 det each,time
R5651 T6290 T6276 npadvmod time,washed
R5652 T6291 T6276 punct ",",washed
R5653 T6292 T6276 cc and,washed
R5654 T6293 T6276 conj incubated,washed
R5655 T6294 T6293 prep with,incubated
R5656 T6295 T6297 det a,dilution
R5657 T6296 T6297 nummod 1/3000,dilution
R5658 T6297 T6294 pobj dilution,with
R5659 T6298 T6297 prep of,dilution
R5660 T6299 T6300 amod HRP-conjugated,F
R5661 T6300 T6298 pobj F,of
R5662 T6301 T6308 punct (,IgG
R5663 T6302 T6306 nmod ab,goat
R5664 T6303 T6302 case ',ab
R5665 T6304 T6302 punct ),ab
R5666 T6305 T6302 nummod 2,ab
R5667 T6306 T6308 nmod goat,IgG
R5668 T6307 T6308 compound anti-rabbit,IgG
R5669 T6308 T6314 nmod IgG,milk
R5670 T6309 T6308 prep in,IgG
R5671 T6310 T6311 nummod 5,%
R5672 T6311 T6314 nmod %,milk
R5673 T6312 T6314 amod nonfat,milk
R5674 T6313 T6314 amod dry,milk
R5675 T6314 T6297 appos milk,dilution
R5676 T6315 T6314 cc and,milk
R5677 T6316 T6317 nummod 0.05,%
R5678 T6317 T6314 conj %,milk
R5679 T6318 T6319 nummod Tween,20
R5680 T6319 T6322 dep 20,for
R5681 T6320 T6319 prep in,20
R5682 T6321 T6320 pobj PBS,in
R5683 T6322 T6293 conj for,incubated
R5684 T6323 T6324 nummod 1,hour
R5685 T6324 T6322 pobj hour,for
R5686 T6325 T6322 prep at,for
R5687 T6326 T6327 compound room,temperature
R5688 T6327 T6325 pobj temperature,at
R5689 T6328 T6276 punct .,washed
R5690 T6329 T6345 prep After,detected
R5691 T6330 T6329 pcomp washing,After
R5692 T6331 T6333 nummod six,times
R5693 T6332 T6331 amod more,six
R5694 T6333 T6330 dobj times,washing
R5695 T6334 T6330 prep in,washing
R5696 T6335 T6334 pobj PBS,in
R5697 T6336 T6330 prep with,washing
R5698 T6337 T6338 nummod 0.05,%
R5699 T6338 T6345 nsubj %,detected
R5700 T6339 T6340 nummod Tween,20
R5701 T6340 T6345 dep 20,detected
R5702 T6341 T6345 punct ",",detected
R5703 T6342 T6343 amod antibody-reactive,proteins
R5704 T6343 T6345 nsubjpass proteins,detected
R5705 T6344 T6345 auxpass were,detected
R5706 T6345 T6345 ROOT detected,detected
R5707 T6346 T6345 advcl using,detected
R5708 T6347 T6349 det a,substrate
R5709 T6348 T6349 compound chemiluminescence,substrate
R5710 T6349 T6346 dobj substrate,using
R5711 T6350 T6359 punct (,according
R5712 T6351 T6359 dep SuperSignal,according
R5713 T6352 T6351 punct ;,SuperSignal
R5714 T6353 T6351 conj Pierce,SuperSignal
R5715 T6354 T6353 punct ",",Pierce
R5716 T6355 T6353 conj Rockford,Pierce
R5717 T6356 T6355 punct ",",Rockford
R5718 T6357 T6355 conj IL,Rockford
R5719 T6358 T6359 punct ),according
R5720 T6359 T6345 prep according,detected
R5721 T6360 T6359 prep to,according
R5722 T6361 T6362 det the,manufacturer
R5723 T6362 T6364 poss manufacturer,instructions
R5724 T6363 T6362 case 's,manufacturer
R5725 T6364 T6360 pobj instructions,to
R5726 T6365 T6345 punct .,detected
R5727 T6366 T6367 aux To,confirm
R5728 T6367 T6380 advcl confirm,were
R5729 T6368 T6374 mark that,loaded
R5730 T6369 T6370 amod equivalent,amounts
R5731 T6370 T6374 nsubjpass amounts,loaded
R5732 T6371 T6370 prep of,amounts
R5733 T6372 T6371 pobj protein,of
R5734 T6373 T6374 auxpass were,loaded
R5735 T6374 T6367 ccomp loaded,confirm
R5736 T6375 T6374 prep in,loaded
R5737 T6376 T6377 det each,line
R5738 T6377 T6375 pobj line,in
R5739 T6378 T6380 punct ",",were
R5740 T6379 T6380 nsubj membranes,were
R5741 T6380 T6380 ROOT were,were
R5742 T6381 T6380 advmod also,were
R5743 T6382 T6383 amod Western,blotted
R5744 T6383 T6380 attr blotted,were
R5745 T6384 T6383 prep for,blotted
R5746 T6385 T6384 pobj ERK,for
R5747 T6386 T6387 mark as,described
R5748 T6387 T6380 advcl described,were
R5749 T6388 T6390 nmod [,]
R5750 T6389 T6390 nummod 32,]
R5751 T6390 T6387 dobj ],described
R5752 T6391 T6380 punct .,were

UBERON-AE

Id Subject Object Predicate Lexical cue
T5473 1973-1977 http://purl.obolibrary.org/obo/UBERON_0001913 denotes milk

sentences

Id Subject Object Predicate Lexical cue
T5475 0-21 Sentence denotes Western Blot Analysis
T5476 22-121 Sentence denotes THP-1 cells were stimulated for various lengths of time with 0.1 mg/ml C10 or C40, or 10 µg/ml LPS.
T5477 122-269 Sentence denotes Cells were then pelleted, washed and homogenised in lysis buffer (10 mM Hepes, pH 7.9, 150 mM NaCl, 1 mM EDTA, 0.6% NP-40, and 0.5 mM PMSF) on ice.
T5478 270-377 Sentence denotes Homogenates were sonicated, centrifuged at 10,000 rpm to remove cellular debris, and supernatant collected.
T5479 378-452 Sentence denotes Protein concentration was determined using the DC Protein Assay (Bio-Rad).
T5480 453-594 Sentence denotes Proteins in samples (15 µg total proteins) were resolved in a denaturing 12% polyacrylamide gel and transferred to a nitrocellulose membrane.
T5481 595-798 Sentence denotes I-κBα protein was detected using a rabbit polyclonal antibody (Santa Cruz Biotechnology, CA) followed by a horseradish peroxidase-coupled goat polyclonal antibody against rabbit Ig (Caltag Laboratories).
T5482 799-956 Sentence denotes Finally, IκB bands were revealed using the ECL™ detection system (Amersham Pharmacia Biotech, Les Ullis, France) according to the manufacturers' instruction.
T5483 957-1019 Sentence denotes Antibody to α-Tubulin (Santa Cruz) was use as loading control.
T5484 1020-1179 Sentence denotes For nuclear NF-κB, THP-1 cells were stimulated with 1 mg/ml C10 or C40 for 30 minutes at 37°C. Cells were then pelleted and nuclei separated as described [31].
T5485 1180-1457 Sentence denotes Nuclei were washed and homogenized directly in loading (Laemli) buffer and heated for 5 minutes at 100°C. Proteins in samples were resolved in a denaturing 8% polyacrylamide gel and transferred to a polyvinylidine fluoride (PVDF) membrane (Immobilon-P; Millipore, Bedford, MA).
T5486 1458-1553 Sentence denotes Membranes were incubated in blocking buffer (1% BSA, in PBS) for two hours at room temperature.
T5487 1554-1651 Sentence denotes Membranes were subsequently probed with the corresponding antibody in blocking buffer, overnight.
T5488 1652-1790 Sentence denotes Rabbit polyclonal antibody anti-NF-κB p50 subunit (# sc-114) or anti-NF-κB p65 subunit (# sc-109) from Santa Cruz Biotechnology were used.
T5489 1791-2035 Sentence denotes Membranes were washed six times in PBS with 0.05% Tween 20, 5 minutes each time, and incubated with a 1/3000 dilution of HRP-conjugated F(ab')2 goat anti-rabbit IgG in 5% nonfat dry milk and 0.05% Tween 20 in PBS for 1 hour at room temperature.
T5490 2036-2251 Sentence denotes After washing six more times in PBS with 0.05% Tween 20, antibody-reactive proteins were detected using a chemiluminescence substrate (SuperSignal; Pierce, Rockford, IL) according to the manufacturer's instructions.
T5491 2252-2386 Sentence denotes To confirm that equivalent amounts of protein were loaded in each line, membranes were also Western blotted for ERK as described [32].
T112 0-21 Sentence denotes Western Blot Analysis
T113 22-121 Sentence denotes THP-1 cells were stimulated for various lengths of time with 0.1 mg/ml C10 or C40, or 10 µg/ml LPS.
T114 122-269 Sentence denotes Cells were then pelleted, washed and homogenised in lysis buffer (10 mM Hepes, pH 7.9, 150 mM NaCl, 1 mM EDTA, 0.6% NP-40, and 0.5 mM PMSF) on ice.
T115 270-377 Sentence denotes Homogenates were sonicated, centrifuged at 10,000 rpm to remove cellular debris, and supernatant collected.
T116 378-452 Sentence denotes Protein concentration was determined using the DC Protein Assay (Bio-Rad).
T117 453-594 Sentence denotes Proteins in samples (15 µg total proteins) were resolved in a denaturing 12% polyacrylamide gel and transferred to a nitrocellulose membrane.
T118 595-798 Sentence denotes I-κBα protein was detected using a rabbit polyclonal antibody (Santa Cruz Biotechnology, CA) followed by a horseradish peroxidase-coupled goat polyclonal antibody against rabbit Ig (Caltag Laboratories).
T119 799-956 Sentence denotes Finally, IκB bands were revealed using the ECL™ detection system (Amersham Pharmacia Biotech, Les Ullis, France) according to the manufacturers' instruction.
T120 957-1019 Sentence denotes Antibody to α-Tubulin (Santa Cruz) was use as loading control.
T121 1020-1114 Sentence denotes For nuclear NF-κB, THP-1 cells were stimulated with 1 mg/ml C10 or C40 for 30 minutes at 37°C.
T122 1115-1179 Sentence denotes Cells were then pelleted and nuclei separated as described [31].
T123 1180-1285 Sentence denotes Nuclei were washed and homogenized directly in loading (Laemli) buffer and heated for 5 minutes at 100°C.
T124 1286-1457 Sentence denotes Proteins in samples were resolved in a denaturing 8% polyacrylamide gel and transferred to a polyvinylidine fluoride (PVDF) membrane (Immobilon-P; Millipore, Bedford, MA).
T125 1458-1553 Sentence denotes Membranes were incubated in blocking buffer (1% BSA, in PBS) for two hours at room temperature.
T126 1554-1651 Sentence denotes Membranes were subsequently probed with the corresponding antibody in blocking buffer, overnight.
T127 1652-1790 Sentence denotes Rabbit polyclonal antibody anti-NF-κB p50 subunit (# sc-114) or anti-NF-κB p65 subunit (# sc-109) from Santa Cruz Biotechnology were used.
T128 1791-2035 Sentence denotes Membranes were washed six times in PBS with 0.05% Tween 20, 5 minutes each time, and incubated with a 1/3000 dilution of HRP-conjugated F(ab')2 goat anti-rabbit IgG in 5% nonfat dry milk and 0.05% Tween 20 in PBS for 1 hour at room temperature.
T129 2036-2251 Sentence denotes After washing six more times in PBS with 0.05% Tween 20, antibody-reactive proteins were detected using a chemiluminescence substrate (SuperSignal; Pierce, Rockford, IL) according to the manufacturer's instructions.
T130 2252-2386 Sentence denotes To confirm that equivalent amounts of protein were loaded in each line, membranes were also Western blotted for ERK as described [32].

events-check-again

Id Subject Object Predicate Lexical cue
T6440 595-600 Protein denotes I-κBα

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T6441 447-450 Protein denotes Rad
T6442 595-608 Protein denotes I-κBα protein
T6443 702-724 Protein denotes horseradish peroxidase
T6444 808-811 Protein denotes IκB
T6445 969-978 Protein denotes α-Tubulin
T6446 1024-1037 Protein denotes nuclear NF-κB
T6447 1652-1683 Protein denotes Rabbit polyclonal antibody anti
T6448 1684-1689 Protein denotes NF-κB
T6449 1690-1701 Protein denotes p50 subunit
T6450 1716-1738 Protein denotes anti-NF-κB p65 subunit
T6451 1912-1955 Protein denotes HRP-conjugated F(ab')2 goat anti-rabbit IgG
T6452 2364-2367 Protein denotes ERK

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T5474 595-600 Protein denotes I-κBα

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T5928 201-203 P0A7Z4 denotes pH
T5929 1690-1693 P19838 denotes p50
T5930 1690-1701 P19838 denotes p50 subunit
T5931 1727-1730 Q04206 denotes p65
T5932 1727-1730 P21579 denotes p65

test2

Id Subject Object Predicate Lexical cue
T5472 595-600 Protein denotes I-κBα