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PMC:2800179 / 11308-12443 JSONTXT

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GO-MF

Id Subject Object Predicate Lexical cue
T5200 0-2 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T5201 181-194 http://purl.obolibrary.org/obo/GO_0003899 denotes transcriptase
T5202 181-194 http://purl.obolibrary.org/obo/GO_0003968 denotes transcriptase
T5203 181-194 http://purl.obolibrary.org/obo/GO_0034062 denotes transcriptase

GO-BP

Id Subject Object Predicate Lexical cue
T5196 0-2 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T5197 181-194 http://purl.obolibrary.org/obo/GO_0003899 denotes transcriptase
T5198 181-194 http://purl.obolibrary.org/obo/GO_0003968 denotes transcriptase
T5199 181-194 http://purl.obolibrary.org/obo/GO_0034062 denotes transcriptase

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T4750 0-6 NN denotes RT-PCR
T4751 7-15 NN denotes Analysis
T4752 16-21 JJ denotes Total
T4753 22-25 NN denotes RNA
T4754 26-30 IN denotes from
T4755 31-40 NN denotes monocytes
T4756 41-44 VB denotes was
T4757 45-53 VB denotes isolated
T4758 54-59 VB denotes using
T4759 60-66 NN denotes TRIzol
T4760 67-75 NN denotes Reagent™
T4761 76-77 -LRB- denotes (
T4762 77-87 NN denotes Invitrogen
T4763 87-88 -RRB- denotes )
T4764 88-92 NN denotes . cD
T4765 92-95 VB denotes NA
T4766 95-104 VB denotes was gener
T4767 104-106 IN denotes at
T4768 106-107 CD denotes e
T4769 107-109 NN denotes d
T4770 109-111 IN denotes on
T4771 112-117 JJ denotes 1 µg
T4772 117-120 NN denotes of
T4773 120-122 IN denotes to
T4774 122-123 DT denotes t
T4775 123-131 NN denotes al RNA i
T4776 131-137 NN denotes n a re
T4777 137-139 IN denotes ac
T4778 139-141 CD denotes ti
T4779 141-143 NN denotes on
T4780 144-145 -COMMA- denotes v
T4781 145-150 VB denotes olume
T4782 151-156 NN denotes of 20
T4783 157-164 JJ denotes µl, usi
T4784 164-177 NN denotes ng M-MLV reve
T4785 177-178 -LRB- denotes r
T4786 178-188 NN denotes se transcr
T4787 188-189 -RRB- denotes i
T4788 209-212 NN denotes PCR
T4789 213-216 VB denotes was
T4790 217-221 VB denotes done
T4791 222-224 IN denotes in
T4792 225-228 DT denotes the
T4793 229-235 JJ denotes linear
T4794 236-241 NN denotes range
T4795 242-244 IN denotes of
T4796 245-258 NN denotes amplification
T4797 259-260 -LRB- denotes (
T4798 260-270 VB denotes determined
T4799 271-274 IN denotes for
T4800 275-279 DT denotes each
T4801 280-286 NN denotes primer
T4802 287-296 NN denotes pair-cDNA
T4803 297-308 NN denotes combination
T4804 308-309 -RRB- denotes )
T4805 311-319 JJ denotes Standard
T4806 320-323 NN denotes PCR
T4807 324-333 NN denotes reactions
T4808 334-338 VB denotes were
T4809 339-348 VB denotes performed
T4810 349-353 IN denotes with
T4811 354-355 CD denotes 1
T4812 356-358 NN denotes µl
T4813 359-361 IN denotes of
T4814 362-365 DT denotes the
T4815 366-370 NN denotes cDNA
T4816 371-379 NN denotes solution
T4817 379-380 -COMMA- denotes ,
T4818 381-383 CD denotes 50
T4819 384-386 NN denotes µM
T4820 387-389 IN denotes of
T4821 390-394 DT denotes each
T4822 395-401 NN denotes primer
T4823 402-410 NN denotes solution
T4824 410-411 -COMMA- denotes ,
T4825 412-414 CD denotes 10
T4826 415-417 NN denotes mM
T4827 418-420 IN denotes of
T4828 421-425 DT denotes each
T4829 426-430 NN denotes dNTP
T4830 430-431 -COMMA- denotes ,
T4831 432-434 CD denotes 25
T4832 435-437 NN denotes mM
T4833 438-443 NN denotes MgCl2
T4834 443-444 -COMMA- denotes ,
T4835 445-448 NNP denotes 10X
T4836 449-457 NNP denotes Goldstar
T4837 458-461 NN denotes DNA
T4838 462-472 NN denotes polymerase
T4839 473-481 NN denotes reaction
T4840 482-488 NN denotes buffer
T4841 488-489 -COMMA- denotes ,
T4842 490-493 CC denotes and
T4843 494-497 CD denotes 0.5
T4844 498-503 NN denotes units
T4845 504-506 IN denotes of
T4846 507-515 NNP denotes Goldstar
T4847 516-519 NN denotes DNA
T4848 520-530 NN denotes polymerase
T4849 531-532 -LRB- denotes (
T4850 532-542 NNP denotes Eurogentec
T4851 542-543 -COMMA- denotes ,
T4852 544-551 NNP denotes Seraing
T4853 551-552 -COMMA- denotes ,
T4854 553-560 NNP denotes Belgium
T4855 560-561 -RRB- denotes )
T4856 563-568 JJ denotes First
T4857 569-572 NN denotes PCR
T4858 573-578 NN denotes cycle
T4859 579-588 VB denotes consisted
T4860 589-591 IN denotes of
T4861 592-593 CD denotes 1
T4862 594-597 NN denotes min
T4863 598-600 IN denotes at
T4864 601-605 NN denotes 92°C
T4865 605-606 -COMMA- denotes ,
T4866 607-608 CD denotes 1
T4867 609-612 NN denotes min
T4868 613-615 IN denotes at
T4869 616-620 NN denotes 58°C
T4870 621-624 CC denotes and
T4871 625-626 CD denotes 1
T4872 627-630 NN denotes min
T4873 631-633 IN denotes at
T4874 634-638 NN denotes 72°C
T4875 638-639 -COLON- denotes ;
T4876 640-644 RB denotes then
T4877 645-649 DT denotes each
T4878 650-653 NN denotes PCR
T4879 654-659 NN denotes cycle
T4880 660-669 VB denotes consisted
T4881 670-672 IN denotes of
T4882 673-675 CD denotes 40
T4883 676-679 NN denotes sec
T4884 680-682 IN denotes at
T4885 683-687 NN denotes 92°C
T4886 687-688 -COMMA- denotes ,
T4887 689-691 CD denotes 40
T4888 692-695 NN denotes sec
T4889 696-698 IN denotes at
T4890 699-703 NN denotes 58°C
T4891 704-707 CC denotes and
T4892 708-710 CD denotes 50
T4893 711-714 NN denotes sec
T4894 715-717 IN denotes at
T4895 718-723 NNP denotes 72°C.
T4896 724-728 NN denotes cDNA
T4897 729-732 IN denotes for
T4898 733-740 NN denotes β-actin
T4899 741-744 VB denotes was
T4900 745-754 VB denotes amplified
T4901 755-758 IN denotes for
T4902 759-761 CD denotes 28
T4903 762-768 NN denotes cycles
T4904 769-774 VB denotes using
T4905 775-778 DT denotes the
T4906 779-785 NN denotes oligos
T4907 785-786 -COLON- denotes :
T4908 787-792 NN denotes sense
T4909 793-818 NN denotes 5′-GGCATCGTGATGGACTCCG-3′
T4910 819-822 CC denotes and
T4911 823-832 JJ denotes antisense
T4912 833-858 JJ denotes 5′GCTGGAAGGTGGACAGCGA-3′.
T4913 859-863 NN denotes cDNA
T4914 864-867 IN denotes for
T4915 868-873 NN denotes TNF-α
T4916 874-877 VB denotes was
T4917 878-887 VB denotes amplified
T4918 888-891 IN denotes for
T4919 892-894 CD denotes 35
T4920 895-901 NN denotes cycles
T4921 902-907 VB denotes using
T4922 908-911 DT denotes the
T4923 912-918 NN denotes oligos
T4924 918-919 -COLON- denotes :
T4925 920-925 NN denotes sense
T4926 926-952 NN denotes 5′-AAGCCTGTAGCCCATGTTGT-3′
T4927 953-956 CC denotes and
T4928 957-966 JJ denotes antisense
T4929 967-994 JJ denotes 5′-CAGATAGATGGGCTCATACC-3′.
T4930 995-999 NN denotes cDNA
T4931 1000-1003 IN denotes for
T4932 1004-1010 NN denotes ICAM-1
T4933 1011-1014 VB denotes was
T4934 1015-1024 VB denotes amplified
T4935 1025-1028 IN denotes for
T4936 1029-1031 CD denotes 35
T4937 1032-1038 NN denotes cycles
T4938 1039-1044 VB denotes using
T4939 1045-1048 DT denotes the
T4940 1049-1055 NN denotes oligos
T4941 1056-1061 VB denotes sense
T4942 1062-1090 NN denotes 5′-GTAGCAGCCGCAGTCATAATGG-3′
T4943 1091-1094 CC denotes and
T4944 1095-1104 JJ denotes antisense
T4945 1105-1109 NN denotes 5′-A
T4946 1110-1134 NN denotes TGCTGTTGTATCTGACTGAGG-3′
R4213 T4751 T4750 arg1Of Analysis,RT-PCR
R4214 T4753 T4752 arg1Of RNA,Total
R4215 T4753 T4754 arg1Of RNA,from
R4216 T4753 T4756 arg1Of RNA,was
R4217 T4753 T4757 arg2Of RNA,isolated
R4218 T4755 T4754 arg2Of monocytes,from
R4219 T4757 T4756 arg2Of isolated,was
R4220 T4758 T4757 arg3Of using,isolated
R4221 T4760 T4758 arg2Of Reagent™,using
R4222 T4760 T4759 arg1Of Reagent™,TRIzol
R4223 T4760 T4761 arg1Of Reagent™,(
R4224 T4762 T4761 arg2Of Invitrogen,(
R4225 T4763 T4761 arg3Of ),(
R4226 T4764 T4765 arg1Of . cD,NA
R4227 T4764 T4766 arg2Of . cD,was gener
R4228 T4766 T4756 modOf was gener,was
R4229 T4766 T4765 arg2Of was gener,NA
R4230 T4766 T4767 arg1Of was gener,at
R4231 T4766 T4780 arg1Of was gener,v
R4232 T4769 T4767 arg2Of d ,at
R4233 T4769 T4768 arg1Of d ,e
R4234 T4769 T4770 arg1Of d ,on
R4235 T4769 T4773 arg1Of d ,to
R4236 T4772 T4770 arg2Of of ,on
R4237 T4772 T4771 arg1Of of ,1 µg
R4238 T4776 T4773 arg2Of n a re,to
R4239 T4776 T4774 arg1Of n a re,t
R4240 T4776 T4775 arg1Of n a re,al RNA i
R4241 T4776 T4777 arg1Of n a re,ac
R4242 T4779 T4777 arg2Of on,ac
R4243 T4779 T4778 arg1Of on,ti
R4244 T4781 T4766 arg3Of olume,was gener
R4245 T4784 T4781 arg2Of ng M-MLV reve,olume
R4246 T4784 T4782 arg1Of ng M-MLV reve,of 20
R4247 T4784 T4783 arg1Of ng M-MLV reve,"µl, usi"
R4248 T4784 T4785 arg1Of ng M-MLV reve,r
R4249 T4786 T4785 arg2Of se transcr,r
R4250 T4787 T4785 arg3Of i,r
R4251 T4788 T4789 arg1Of PCR,was
R4252 T4788 T4790 arg2Of PCR,done
R4253 T4790 T4789 arg2Of done,was
R4254 T4790 T4791 arg1Of done,in
R4255 T4794 T4791 arg2Of range,in
R4256 T4794 T4792 arg1Of range,the
R4257 T4794 T4793 arg1Of range,linear
R4258 T4794 T4795 arg1Of range,of
R4259 T4796 T4795 arg2Of amplification,of
R4260 T4796 T4797 arg1Of amplification,(
R4261 T4798 T4797 arg2Of determined,(
R4262 T4798 T4799 arg1Of determined,for
R4263 T4803 T4799 arg2Of combination,for
R4264 T4803 T4800 arg1Of combination,each
R4265 T4803 T4801 arg1Of combination,primer
R4266 T4803 T4802 arg1Of combination,pair-cDNA
R4267 T4804 T4797 arg3Of ),(
R4268 T4807 T4805 arg1Of reactions,Standard
R4269 T4807 T4806 arg1Of reactions,PCR
R4270 T4807 T4808 arg1Of reactions,were
R4271 T4807 T4809 arg2Of reactions,performed
R4272 T4809 T4808 arg2Of performed,were
R4273 T4809 T4810 arg1Of performed,with
R4274 T4809 T4849 arg1Of performed,(
R4275 T4812 T4811 arg1Of µl,1
R4276 T4812 T4813 arg1Of µl,of
R4277 T4812 T4817 arg1Of µl,","
R4278 T4816 T4813 arg2Of solution,of
R4279 T4816 T4814 arg1Of solution,the
R4280 T4816 T4815 arg1Of solution,cDNA
R4281 T4817 T4824 arg1Of ",",","
R4282 T4819 T4817 arg2Of µM,","
R4283 T4819 T4818 arg1Of µM,50
R4284 T4819 T4820 arg1Of µM,of
R4285 T4823 T4820 arg2Of solution,of
R4286 T4823 T4821 arg1Of solution,each
R4287 T4823 T4822 arg1Of solution,primer
R4288 T4824 T4830 arg1Of ",",","
R4289 T4826 T4824 arg2Of mM,","
R4290 T4826 T4825 arg1Of mM,10
R4291 T4826 T4827 arg1Of mM,of
R4292 T4829 T4827 arg2Of dNTP,of
R4293 T4829 T4828 arg1Of dNTP,each
R4294 T4830 T4834 arg1Of ",",","
R4295 T4830 T4842 arg1Of ",",and
R4296 T4831 T4832 arg1Of 25,mM
R4297 T4833 T4830 arg2Of MgCl2,","
R4298 T4833 T4831 arg1Of MgCl2,25
R4299 T4836 T4835 arg1Of Goldstar,10X
R4300 T4840 T4834 arg2Of buffer,","
R4301 T4840 T4836 arg1Of buffer,Goldstar
R4302 T4840 T4837 arg1Of buffer,DNA
R4303 T4840 T4838 arg1Of buffer,polymerase
R4304 T4840 T4839 arg1Of buffer,reaction
R4305 T4842 T4810 arg2Of and,with
R4306 T4842 T4841 arg1Of and,","
R4307 T4844 T4842 arg2Of units,and
R4308 T4844 T4843 arg1Of units,0.5
R4309 T4844 T4845 arg1Of units,of
R4310 T4848 T4845 arg2Of polymerase,of
R4311 T4848 T4846 arg1Of polymerase,Goldstar
R4312 T4848 T4847 arg1Of polymerase,DNA
R4313 T4850 T4851 arg1Of Eurogentec,","
R4314 T4851 T4849 arg2Of ",",(
R4315 T4851 T4853 arg1Of ",",","
R4316 T4852 T4851 arg2Of Seraing,","
R4317 T4854 T4853 arg2Of Belgium,","
R4318 T4855 T4849 arg3Of ),(
R4319 T4858 T4856 arg1Of cycle,First
R4320 T4858 T4857 arg1Of cycle,PCR
R4321 T4858 T4859 arg1Of cycle,consisted
R4322 T4859 T4860 arg1Of consisted,of
R4323 T4859 T4863 arg1Of consisted,at
R4324 T4859 T4875 arg1Of consisted,;
R4325 T4862 T4860 arg2Of min,of
R4326 T4862 T4861 arg1Of min,1
R4327 T4864 T4865 arg1Of 92°C,","
R4328 T4865 T4870 arg1Of ",",and
R4329 T4867 T4865 arg2Of min,","
R4330 T4867 T4866 arg1Of min,1
R4331 T4867 T4868 arg1Of min,at
R4332 T4869 T4868 arg2Of 58°C,at
R4333 T4870 T4863 arg2Of and,at
R4334 T4870 T4873 arg1Of and,at
R4335 T4872 T4870 arg2Of min,and
R4336 T4872 T4871 arg1Of min,1
R4337 T4874 T4873 arg2Of 72°C,at
R4338 T4879 T4877 arg1Of cycle,each
R4339 T4879 T4878 arg1Of cycle,PCR
R4340 T4879 T4880 arg1Of cycle,consisted
R4341 T4880 T4875 arg2Of consisted,;
R4342 T4880 T4876 arg1Of consisted,then
R4343 T4880 T4881 arg1Of consisted,of
R4344 T4880 T4884 arg1Of consisted,at
R4345 T4883 T4881 arg2Of sec,of
R4346 T4883 T4882 arg1Of sec,40
R4347 T4885 T4884 arg2Of 92°C,at
R4348 T4888 T4887 arg1Of sec,40
R4349 T4888 T4889 arg1Of sec,at
R4350 T4888 T4894 arg1Of sec,at
R4351 T4888 T4899 arg1Of sec,was
R4352 T4888 T4900 arg2Of sec,amplified
R4353 T4888 T4941 arg1Of sec,sense
R4354 T4890 T4891 arg1Of 58°C,and
R4355 T4891 T4889 arg2Of and,at
R4356 T4893 T4891 arg2Of sec,and
R4357 T4893 T4892 arg1Of sec,50
R4358 T4896 T4894 arg2Of cDNA,at
R4359 T4896 T4895 arg1Of cDNA,72°C.
R4360 T4896 T4897 arg1Of cDNA,for
R4361 T4898 T4897 arg2Of β-actin,for
R4362 T4900 T4899 arg2Of amplified,was
R4363 T4900 T4901 arg1Of amplified,for
R4364 T4900 T4907 arg1Of amplified,:
R4365 T4903 T4901 arg2Of cycles,for
R4366 T4903 T4902 arg1Of cycles,28
R4367 T4903 T4904 arg1Of cycles,using
R4368 T4906 T4904 arg2Of oligos,using
R4369 T4906 T4905 arg1Of oligos,the
R4370 T4907 T4875 arg3Of :,;
R4371 T4907 T4886 arg1Of :,","
R4372 T4909 T4908 arg1Of 5′-GGCATCGTGATGGACTCCG-3′,sense
R4373 T4909 T4910 arg1Of 5′-GGCATCGTGATGGACTCCG-3′,and
R4374 T4910 T4914 arg1Of and,for
R4375 T4910 T4916 arg1Of and,was
R4376 T4910 T4917 arg2Of and,amplified
R4377 T4913 T4910 arg2Of cDNA,and
R4378 T4913 T4911 arg1Of cDNA,antisense
R4379 T4913 T4912 arg1Of cDNA,5′GCTGGAAGGTGGACAGCGA-3′.
R4380 T4915 T4914 arg2Of TNF-α,for
R4381 T4917 T4916 arg2Of amplified,was
R4382 T4917 T4918 arg1Of amplified,for
R4383 T4917 T4924 arg1Of amplified,:
R4384 T4920 T4918 arg2Of cycles,for
R4385 T4920 T4919 arg1Of cycles,35
R4386 T4920 T4921 arg1Of cycles,using
R4387 T4923 T4921 arg2Of oligos,using
R4388 T4923 T4922 arg1Of oligos,the
R4389 T4926 T4925 arg1Of 5′-AAGCCTGTAGCCCATGTTGT-3′,sense
R4390 T4926 T4927 arg1Of 5′-AAGCCTGTAGCCCATGTTGT-3′,and
R4391 T4927 T4931 arg1Of and,for
R4392 T4927 T4933 arg1Of and,was
R4393 T4927 T4934 arg2Of and,amplified
R4394 T4930 T4927 arg2Of cDNA,and
R4395 T4930 T4928 arg1Of cDNA,antisense
R4396 T4930 T4929 arg1Of cDNA,5′-CAGATAGATGGGCTCATACC-3′.
R4397 T4932 T4931 arg2Of ICAM-1,for
R4398 T4934 T4924 arg2Of amplified,:
R4399 T4934 T4933 arg2Of amplified,was
R4400 T4934 T4935 arg1Of amplified,for
R4401 T4937 T4935 arg2Of cycles,for
R4402 T4937 T4936 arg1Of cycles,35
R4403 T4937 T4938 arg1Of cycles,using
R4404 T4940 T4938 arg2Of oligos,using
R4405 T4940 T4939 arg1Of oligos,the
R4406 T4941 T4907 arg2Of sense,:
R4407 T4941 T4916 modOf sense,was
R4408 T4942 T4943 arg1Of 5′-GTAGCAGCCGCAGTCATAATGG-3′,and
R4409 T4944 T4943 arg2Of antisense,and
R4410 T4946 T4941 arg2Of TGCTGTTGTATCTGACTGAGG-3′,sense
R4411 T4946 T4942 arg1Of TGCTGTTGTATCTGACTGAGG-3′,5′-GTAGCAGCCGCAGTCATAATGG-3′
R4412 T4946 T4944 arg1Of TGCTGTTGTATCTGACTGAGG-3′,antisense
R4413 T4946 T4945 arg1Of TGCTGTTGTATCTGACTGAGG-3′,5′-A

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T4951 0-6 NNP denotes RT-PCR
T4952 7-15 NNP denotes Analysis
T4953 16-21 NNP denotes Total
T4954 22-25 NNP denotes RNA
T4955 26-30 IN denotes from
T4956 31-40 NNS denotes monocytes
T4957 41-44 VBD denotes was
T4958 45-53 VBN denotes isolated
T4959 54-59 VBG denotes using
T4960 60-66 NNP denotes TRIzol
T4961 67-74 NNP denotes Reagent
T4962 74-75 NNP denotes ™
T4963 76-77 -LRB- denotes (
T4964 77-87 NNP denotes Invitrogen
T4965 87-88 -RRB- denotes )
T4966 88-89 . denotes .
T4967 90-94 NNP denotes cDNA
T4968 95-98 VBD denotes was
T4969 99-108 VBN denotes generated
T4970 109-111 IN denotes on
T4971 112-113 CD denotes 1
T4972 114-116 NN denotes µg
T4973 117-119 IN denotes of
T4974 120-125 JJ denotes total
T4975 126-129 NNP denotes RNA
T4976 130-132 IN denotes in
T4977 133-134 DT denotes a
T4978 135-143 NN denotes reaction
T4979 144-150 NN denotes volume
T4980 151-153 IN denotes of
T4981 154-156 CD denotes 20
T4982 157-159 NN denotes µl
T4983 159-160 , denotes ,
T4984 161-166 VBG denotes using
T4985 167-172 NNP denotes M-MLV
T4986 173-180 VB denotes reverse
T4987 181-194 NN denotes transcriptase
T4988 195-196 -LRB- denotes (
T4989 196-206 NNP denotes Invitrogen
T4990 206-207 -RRB- denotes )
T4991 207-208 . denotes .
T4992 209-212 NNP denotes PCR
T4993 213-216 VBD denotes was
T4994 217-221 VBN denotes done
T4995 222-224 IN denotes in
T4996 225-228 DT denotes the
T4997 229-235 JJ denotes linear
T4998 236-241 NN denotes range
T4999 242-244 IN denotes of
T5000 245-258 NN denotes amplification
T5001 259-260 -LRB- denotes (
T5002 260-270 VBN denotes determined
T5003 271-274 IN denotes for
T5004 275-279 DT denotes each
T5005 280-286 NN denotes primer
T5006 287-296 NN denotes pair-cDNA
T5007 297-308 NN denotes combination
T5008 308-309 -RRB- denotes )
T5009 309-310 . denotes .
T5010 311-319 NNP denotes Standard
T5011 320-323 NNP denotes PCR
T5012 324-333 NNS denotes reactions
T5013 334-338 VBD denotes were
T5014 339-348 VBN denotes performed
T5015 349-353 IN denotes with
T5016 354-355 CD denotes 1
T5017 356-358 NN denotes µl
T5018 359-361 IN denotes of
T5019 362-365 DT denotes the
T5020 366-370 NNP denotes cDNA
T5021 371-379 NN denotes solution
T5022 379-380 , denotes ,
T5023 381-383 CD denotes 50
T5024 384-386 NN denotes µM
T5025 387-389 IN denotes of
T5026 390-394 DT denotes each
T5027 395-401 NN denotes primer
T5028 402-410 NN denotes solution
T5029 410-411 , denotes ,
T5030 412-414 CD denotes 10
T5031 415-417 NN denotes mM
T5032 418-420 IN denotes of
T5033 421-425 DT denotes each
T5034 426-430 NNP denotes dNTP
T5035 430-431 , denotes ,
T5036 432-434 CD denotes 25
T5037 435-437 NNP denotes mM
T5038 438-443 NNP denotes MgCl2
T5039 443-444 , denotes ,
T5040 445-448 NNP denotes 10X
T5041 449-457 NNP denotes Goldstar
T5042 458-461 NNP denotes DNA
T5043 462-472 NN denotes polymerase
T5044 473-481 NN denotes reaction
T5045 482-488 NN denotes buffer
T5046 488-489 , denotes ,
T5047 490-493 CC denotes and
T5048 494-497 CD denotes 0.5
T5049 498-503 NNS denotes units
T5050 504-506 IN denotes of
T5051 507-515 NNP denotes Goldstar
T5052 516-519 NNP denotes DNA
T5053 520-530 NN denotes polymerase
T5054 531-532 -LRB- denotes (
T5055 532-542 NNP denotes Eurogentec
T5056 542-543 , denotes ,
T5057 544-551 NNP denotes Seraing
T5058 551-552 , denotes ,
T5059 553-560 NNP denotes Belgium
T5060 560-561 -RRB- denotes )
T5061 561-562 . denotes .
T5062 563-568 NNP denotes First
T5063 569-572 NNP denotes PCR
T5064 573-578 NN denotes cycle
T5065 579-588 VBD denotes consisted
T5066 589-591 IN denotes of
T5067 592-593 CD denotes 1
T5068 594-597 NN denotes min
T5069 598-600 IN denotes at
T5070 601-603 CD denotes 92
T5071 603-604 CD denotes °
T5072 604-605 NNP denotes C
T5073 605-606 , denotes ,
T5074 607-608 CD denotes 1
T5075 609-612 NN denotes min
T5076 613-615 IN denotes at
T5077 616-618 CD denotes 58
T5078 618-619 CD denotes °
T5079 619-620 NNP denotes C
T5080 621-624 CC denotes and
T5081 625-626 CD denotes 1
T5082 627-630 NN denotes min
T5083 631-633 IN denotes at
T5084 634-636 CD denotes 72
T5085 636-637 CD denotes °
T5086 637-638 NNP denotes C
T5087 638-639 : denotes ;
T5088 640-644 RB denotes then
T5089 645-649 DT denotes each
T5090 650-653 NNP denotes PCR
T5091 654-659 NN denotes cycle
T5092 660-669 VBD denotes consisted
T5093 670-672 IN denotes of
T5094 673-675 CD denotes 40
T5095 676-679 NN denotes sec
T5096 680-682 IN denotes at
T5097 683-685 CD denotes 92
T5098 685-686 CD denotes °
T5099 686-687 NNP denotes C
T5100 687-688 , denotes ,
T5101 689-691 CD denotes 40
T5102 692-695 NN denotes sec
T5103 696-698 IN denotes at
T5104 699-701 CD denotes 58
T5105 701-702 CD denotes °
T5106 702-703 NNP denotes C
T5107 704-707 CC denotes and
T5108 708-710 CD denotes 50
T5109 711-714 NN denotes sec
T5110 715-717 IN denotes at
T5111 718-720 CD denotes 72
T5112 720-721 CD denotes °
T5113 721-723 NNP denotes C.
T5114 724-728 NNP denotes cDNA
T5115 729-732 IN denotes for
T5116 733-740 NN denotes β-actin
T5117 741-744 VBD denotes was
T5118 745-754 VBN denotes amplified
T5119 755-758 IN denotes for
T5120 759-761 CD denotes 28
T5121 762-768 NNS denotes cycles
T5122 769-774 VBG denotes using
T5123 775-778 DT denotes the
T5124 779-785 NNS denotes oligos
T5125 785-786 : denotes :
T5126 787-792 NN denotes sense
T5127 793-794 CD denotes 5
T5128 794-795 SYM denotes ′
T5129 795-796 : denotes -
T5130 796-817 NN denotes GGCATCGTGATGGACTCCG-3
T5131 817-818 NN denotes ′
T5132 819-822 CC denotes and
T5133 823-832 JJ denotes antisense
T5134 833-834 CD denotes 5
T5135 834-835 CD denotes ′
T5136 835-856 NNP denotes GCTGGAAGGTGGACAGCGA-3
T5137 856-857 NN denotes ′
T5138 857-858 . denotes .
T5139 859-863 NNP denotes cDNA
T5140 864-867 IN denotes for
T5141 868-873 NNP denotes TNF-α
T5142 874-877 VBD denotes was
T5143 878-887 VBN denotes amplified
T5144 888-891 IN denotes for
T5145 892-894 CD denotes 35
T5146 895-901 NNS denotes cycles
T5147 902-907 VBG denotes using
T5148 908-911 DT denotes the
T5149 912-918 NNS denotes oligos
T5150 918-919 : denotes :
T5151 920-925 NN denotes sense
T5152 926-927 CD denotes 5
T5153 927-928 SYM denotes ′
T5154 928-929 : denotes -
T5155 929-951 NN denotes AAGCCTGTAGCCCATGTTGT-3
T5156 951-952 NN denotes ′
T5157 953-956 CC denotes and
T5158 957-966 JJ denotes antisense
T5159 967-968 CD denotes 5
T5160 968-969 SYM denotes ′
T5161 969-970 : denotes -
T5162 970-992 NN denotes CAGATAGATGGGCTCATACC-3
T5163 992-993 NN denotes ′
T5164 993-994 . denotes .
T5165 995-999 NNP denotes cDNA
T5166 1000-1003 IN denotes for
T5167 1004-1010 NNP denotes ICAM-1
T5168 1011-1014 VBD denotes was
T5169 1015-1024 VBN denotes amplified
T5170 1025-1028 IN denotes for
T5171 1029-1031 CD denotes 35
T5172 1032-1038 NNS denotes cycles
T5173 1039-1044 VBG denotes using
T5174 1045-1048 DT denotes the
T5175 1049-1055 NNS denotes oligos
T5176 1056-1061 VBP denotes sense
T5177 1062-1063 CD denotes 5
T5178 1063-1064 SYM denotes ′
T5179 1064-1065 : denotes -
T5180 1065-1089 NN denotes GTAGCAGCCGCAGTCATAATGG-3
T5181 1089-1090 NN denotes ′
T5182 1091-1094 CC denotes and
T5183 1095-1104 JJ denotes antisense
T5184 1105-1106 CD denotes 5
T5185 1106-1107 SYM denotes ′
T5186 1107-1108 : denotes -
T5187 1108-1109 DT denotes A
T5188 1110-1133 JJ denotes TGCTGTTGTATCTGACTGAGG-3
T5189 1133-1134 NN denotes ′
T5190 1134-1135 . denotes .
R4414 T4951 T4952 compound RT-PCR,Analysis
R4415 T4952 T4958 nsubjpass Analysis,isolated
R4416 T4953 T4954 compound Total,RNA
R4417 T4954 T4952 appos RNA,Analysis
R4418 T4955 T4954 prep from,RNA
R4419 T4956 T4955 pobj monocytes,from
R4420 T4957 T4958 auxpass was,isolated
R4421 T4958 T4958 ROOT isolated,isolated
R4422 T4959 T4958 advcl using,isolated
R4423 T4960 T4962 compound TRIzol,™
R4424 T4961 T4962 compound Reagent,™
R4425 T4962 T4959 dobj ™,using
R4426 T4963 T4964 punct (,Invitrogen
R4427 T4964 T4962 appos Invitrogen,™
R4428 T4965 T4962 punct ),™
R4429 T4966 T4958 punct .,isolated
R4430 T4967 T4969 nsubjpass cDNA,generated
R4431 T4968 T4969 auxpass was,generated
R4432 T4969 T4969 ROOT generated,generated
R4433 T4970 T4969 prep on,generated
R4434 T4971 T4972 nummod 1,µg
R4435 T4972 T4970 pobj µg,on
R4436 T4973 T4972 prep of,µg
R4437 T4974 T4975 amod total,RNA
R4438 T4975 T4973 pobj RNA,of
R4439 T4976 T4969 prep in,generated
R4440 T4977 T4979 det a,volume
R4441 T4978 T4979 compound reaction,volume
R4442 T4979 T4976 pobj volume,in
R4443 T4980 T4979 prep of,volume
R4444 T4981 T4982 nummod 20,µl
R4445 T4982 T4980 pobj µl,of
R4446 T4983 T4969 punct ",",generated
R4447 T4984 T4969 advcl using,generated
R4448 T4985 T4986 compound M-MLV,reverse
R4449 T4986 T4984 dobj reverse,using
R4450 T4987 T4986 npadvmod transcriptase,reverse
R4451 T4988 T4987 punct (,transcriptase
R4452 T4989 T4987 appos Invitrogen,transcriptase
R4453 T4990 T4987 punct ),transcriptase
R4454 T4991 T4969 punct .,generated
R4455 T4992 T4994 nsubjpass PCR,done
R4456 T4993 T4994 auxpass was,done
R4457 T4994 T4994 ROOT done,done
R4458 T4995 T4994 prep in,done
R4459 T4996 T4998 det the,range
R4460 T4997 T4998 amod linear,range
R4461 T4998 T4995 pobj range,in
R4462 T4999 T4998 prep of,range
R4463 T5000 T4999 pobj amplification,of
R4464 T5001 T4998 punct (,range
R4465 T5002 T4994 conj determined,done
R4466 T5003 T5002 prep for,determined
R4467 T5004 T5007 det each,combination
R4468 T5005 T5006 compound primer,pair-cDNA
R4469 T5006 T5007 compound pair-cDNA,combination
R4470 T5007 T5003 pobj combination,for
R4471 T5008 T5002 punct ),determined
R4472 T5009 T4994 punct .,done
R4473 T5010 T5012 amod Standard,reactions
R4474 T5011 T5012 compound PCR,reactions
R4475 T5012 T5014 nsubjpass reactions,performed
R4476 T5013 T5014 auxpass were,performed
R4477 T5014 T5014 ROOT performed,performed
R4478 T5015 T5014 prep with,performed
R4479 T5016 T5017 nummod 1,µl
R4480 T5017 T5015 pobj µl,with
R4481 T5018 T5017 prep of,µl
R4482 T5019 T5021 det the,solution
R4483 T5020 T5021 compound cDNA,solution
R4484 T5021 T5018 pobj solution,of
R4485 T5022 T5021 punct ",",solution
R4486 T5023 T5024 nummod 50,µM
R4487 T5024 T5021 appos µM,solution
R4488 T5025 T5024 prep of,µM
R4489 T5026 T5028 det each,solution
R4490 T5027 T5028 compound primer,solution
R4491 T5028 T5025 pobj solution,of
R4492 T5029 T5024 punct ",",µM
R4493 T5030 T5031 nummod 10,mM
R4494 T5031 T5024 appos mM,µM
R4495 T5032 T5031 prep of,mM
R4496 T5033 T5034 det each,dNTP
R4497 T5034 T5032 pobj dNTP,of
R4498 T5035 T5024 punct ",",µM
R4499 T5036 T5038 nummod 25,MgCl2
R4500 T5037 T5038 compound mM,MgCl2
R4501 T5038 T5024 appos MgCl2,µM
R4502 T5039 T5038 punct ",",MgCl2
R4503 T5040 T5042 compound 10X,DNA
R4504 T5041 T5042 compound Goldstar,DNA
R4505 T5042 T5045 compound DNA,buffer
R4506 T5043 T5045 compound polymerase,buffer
R4507 T5044 T5045 compound reaction,buffer
R4508 T5045 T5038 appos buffer,MgCl2
R4509 T5046 T5038 punct ",",MgCl2
R4510 T5047 T5038 cc and,MgCl2
R4511 T5048 T5049 nummod 0.5,units
R4512 T5049 T5024 appos units,µM
R4513 T5050 T5049 prep of,units
R4514 T5051 T5053 compound Goldstar,polymerase
R4515 T5052 T5053 compound DNA,polymerase
R4516 T5053 T5050 pobj polymerase,of
R4517 T5054 T5055 punct (,Eurogentec
R4518 T5055 T5053 appos Eurogentec,polymerase
R4519 T5056 T5055 punct ",",Eurogentec
R4520 T5057 T5055 conj Seraing,Eurogentec
R4521 T5058 T5057 punct ",",Seraing
R4522 T5059 T5057 conj Belgium,Seraing
R4523 T5060 T5024 punct ),µM
R4524 T5061 T5014 punct .,performed
R4525 T5062 T5064 amod First,cycle
R4526 T5063 T5064 compound PCR,cycle
R4527 T5064 T5065 nsubj cycle,consisted
R4528 T5065 T5092 ccomp consisted,consisted
R4529 T5066 T5065 prep of,consisted
R4530 T5067 T5068 nummod 1,min
R4531 T5068 T5066 pobj min,of
R4532 T5069 T5065 prep at,consisted
R4533 T5070 T5071 nummod 92,°
R4534 T5071 T5069 pobj °,at
R4535 T5072 T5065 npadvmod C,consisted
R4536 T5073 T5072 punct ",",C
R4537 T5074 T5075 nummod 1,min
R4538 T5075 T5072 appos min,C
R4539 T5076 T5075 prep at,min
R4540 T5077 T5078 nummod 58,°
R4541 T5078 T5079 nummod °,C
R4542 T5079 T5076 pobj C,at
R4543 T5080 T5075 cc and,min
R4544 T5081 T5082 nummod 1,min
R4545 T5082 T5075 conj min,min
R4546 T5083 T5082 prep at,min
R4547 T5084 T5085 nummod 72,°
R4548 T5085 T5086 nummod °,C
R4549 T5086 T5083 pobj C,at
R4550 T5087 T5092 punct ;,consisted
R4551 T5088 T5092 advmod then,consisted
R4552 T5089 T5091 det each,cycle
R4553 T5090 T5091 compound PCR,cycle
R4554 T5091 T5092 nsubj cycle,consisted
R4555 T5092 T5092 ROOT consisted,consisted
R4556 T5093 T5092 prep of,consisted
R4557 T5094 T5095 nummod 40,sec
R4558 T5095 T5093 pobj sec,of
R4559 T5096 T5092 prep at,consisted
R4560 T5097 T5098 nummod 92,°
R4561 T5098 T5096 pobj °,at
R4562 T5099 T5092 dobj C,consisted
R4563 T5100 T5099 punct ",",C
R4564 T5101 T5102 nummod 40,sec
R4565 T5102 T5099 appos sec,C
R4566 T5103 T5102 prep at,sec
R4567 T5104 T5105 nummod 58,°
R4568 T5105 T5106 nummod °,C
R4569 T5106 T5103 pobj C,at
R4570 T5107 T5102 cc and,sec
R4571 T5108 T5109 nummod 50,sec
R4572 T5109 T5102 conj sec,sec
R4573 T5110 T5109 prep at,sec
R4574 T5111 T5112 nummod 72,°
R4575 T5112 T5114 nummod °,cDNA
R4576 T5113 T5114 compound C.,cDNA
R4577 T5114 T5110 pobj cDNA,at
R4578 T5115 T5109 prep for,sec
R4579 T5116 T5115 pobj β-actin,for
R4580 T5117 T5118 auxpass was,amplified
R4581 T5118 T5092 conj amplified,consisted
R4582 T5119 T5118 prep for,amplified
R4583 T5120 T5121 nummod 28,cycles
R4584 T5121 T5119 pobj cycles,for
R4585 T5122 T5118 advcl using,amplified
R4586 T5123 T5124 det the,oligos
R4587 T5124 T5122 dobj oligos,using
R4588 T5125 T5124 punct :,oligos
R4589 T5126 T5124 appos sense,oligos
R4590 T5127 T5131 nummod 5,′
R4591 T5128 T5130 punct ′,GGCATCGTGATGGACTCCG-3
R4592 T5129 T5130 punct -,GGCATCGTGATGGACTCCG-3
R4593 T5130 T5131 compound GGCATCGTGATGGACTCCG-3,′
R4594 T5131 T5124 appos ′,oligos
R4595 T5132 T5131 cc and,′
R4596 T5133 T5131 conj antisense,′
R4597 T5134 T5133 nummod 5,antisense
R4598 T5135 T5137 nummod ′,′
R4599 T5136 T5137 compound GCTGGAAGGTGGACAGCGA-3,′
R4600 T5137 T5133 appos ′,antisense
R4601 T5138 T5092 punct .,consisted
R4602 T5139 T5143 nsubjpass cDNA,amplified
R4603 T5140 T5139 prep for,cDNA
R4604 T5141 T5140 pobj TNF-α,for
R4605 T5142 T5143 auxpass was,amplified
R4606 T5143 T5143 ROOT amplified,amplified
R4607 T5144 T5143 prep for,amplified
R4608 T5145 T5146 nummod 35,cycles
R4609 T5146 T5144 pobj cycles,for
R4610 T5147 T5143 advcl using,amplified
R4611 T5148 T5149 det the,oligos
R4612 T5149 T5147 dobj oligos,using
R4613 T5150 T5149 punct :,oligos
R4614 T5151 T5149 appos sense,oligos
R4615 T5152 T5155 nummod 5,AAGCCTGTAGCCCATGTTGT-3
R4616 T5153 T5155 punct ′,AAGCCTGTAGCCCATGTTGT-3
R4617 T5154 T5155 punct -,AAGCCTGTAGCCCATGTTGT-3
R4618 T5155 T5156 compound AAGCCTGTAGCCCATGTTGT-3,′
R4619 T5156 T5149 appos ′,oligos
R4620 T5157 T5156 cc and,′
R4621 T5158 T5163 nmod antisense,′
R4622 T5159 T5158 nummod 5,antisense
R4623 T5160 T5162 punct ′,CAGATAGATGGGCTCATACC-3
R4624 T5161 T5162 punct -,CAGATAGATGGGCTCATACC-3
R4637 T5174 T5176 det the,sense
R4638 T5175 T5176 nsubj oligos,sense
R4639 T5176 T5173 dobj sense,using
R4640 T5177 T5181 nummod 5,′
R4641 T5178 T5180 punct ′,GTAGCAGCCGCAGTCATAATGG-3
R4642 T5179 T5180 punct -,GTAGCAGCCGCAGTCATAATGG-3
R4643 T5180 T5181 compound GTAGCAGCCGCAGTCATAATGG-3,′
R4644 T5181 T5176 appos ′,sense
R4645 T5182 T5181 cc and,′
R4646 T5183 T5181 conj antisense,′
R4647 T5184 T5183 nummod 5,antisense
R4648 T5185 T5189 punct ′,′
R4649 T5186 T5189 punct -,′
R4650 T5187 T5189 det A,′
R4651 T5188 T5189 amod TGCTGTTGTATCTGACTGAGG-3,′
R4652 T5189 T5181 appos ′,′
R4653 T5190 T5169 punct .,amplified
R4625 T5162 T5163 compound CAGATAGATGGGCTCATACC-3,′
R4626 T5163 T5156 conj ′,′
R4627 T5164 T5143 punct .,amplified
R4628 T5165 T5169 nsubjpass cDNA,amplified
R4629 T5166 T5169 prep for,amplified
R4630 T5167 T5166 pobj ICAM-1,for
R4631 T5168 T5169 auxpass was,amplified
R4632 T5169 T5169 ROOT amplified,amplified
R4633 T5170 T5169 prep for,amplified
R4634 T5171 T5172 nummod 35,cycles
R4635 T5172 T5170 pobj cycles,for
R4636 T5173 T5169 advcl using,amplified

sentences

Id Subject Object Predicate Lexical cue
T4745 0-15 Sentence denotes RT-PCR Analysis
T4746 16-208 Sentence denotes Total RNA from monocytes was isolated using TRIzol Reagent™ (Invitrogen). cDNA was generated on 1 µg of total RNA in a reaction volume of 20 µl, using M-MLV reverse transcriptase (Invitrogen).
T4747 209-310 Sentence denotes PCR was done in the linear range of amplification (determined for each primer pair-cDNA combination).
T4748 311-562 Sentence denotes Standard PCR reactions were performed with 1 µl of the cDNA solution, 50 µM of each primer solution, 10 mM of each dNTP, 25 mM MgCl2, 10X Goldstar DNA polymerase reaction buffer, and 0.5 units of Goldstar DNA polymerase (Eurogentec, Seraing, Belgium).
T4749 563-1135 Sentence denotes First PCR cycle consisted of 1 min at 92°C, 1 min at 58°C and 1 min at 72°C; then each PCR cycle consisted of 40 sec at 92°C, 40 sec at 58°C and 50 sec at 72°C. cDNA for β-actin was amplified for 28 cycles using the oligos: sense 5′-GGCATCGTGATGGACTCCG-3′ and antisense 5′GCTGGAAGGTGGACAGCGA-3′. cDNA for TNF-α was amplified for 35 cycles using the oligos: sense 5′-AAGCCTGTAGCCCATGTTGT-3′ and antisense 5′-CAGATAGATGGGCTCATACC-3′. cDNA for ICAM-1 was amplified for 35 cycles using the oligos sense 5′-GTAGCAGCCGCAGTCATAATGG-3′ and antisense 5′-A TGCTGTTGTATCTGACTGAGG-3′.
T100 0-15 Sentence denotes RT-PCR Analysis
T101 16-208 Sentence denotes Total RNA from monocytes was isolated using TRIzol Reagent™ (Invitrogen). cDNA was generated on 1 µg of total RNA in a reaction volume of 20 µl, using M-MLV reverse transcriptase (Invitrogen).
T102 209-310 Sentence denotes PCR was done in the linear range of amplification (determined for each primer pair-cDNA combination).
T103 311-562 Sentence denotes Standard PCR reactions were performed with 1 µl of the cDNA solution, 50 µM of each primer solution, 10 mM of each dNTP, 25 mM MgCl2, 10X Goldstar DNA polymerase reaction buffer, and 0.5 units of Goldstar DNA polymerase (Eurogentec, Seraing, Belgium).
T104 563-1135 Sentence denotes First PCR cycle consisted of 1 min at 92°C, 1 min at 58°C and 1 min at 72°C; then each PCR cycle consisted of 40 sec at 92°C, 40 sec at 58°C and 50 sec at 72°C. cDNA for β-actin was amplified for 28 cycles using the oligos: sense 5′-GGCATCGTGATGGACTCCG-3′ and antisense 5′GCTGGAAGGTGGACAGCGA-3′. cDNA for TNF-α was amplified for 35 cycles using the oligos: sense 5′-AAGCCTGTAGCCCATGTTGT-3′ and antisense 5′-CAGATAGATGGGCTCATACC-3′. cDNA for ICAM-1 was amplified for 35 cycles using the oligos sense 5′-GTAGCAGCCGCAGTCATAATGG-3′ and antisense 5′-A TGCTGTTGTATCTGACTGAGG-3′.

events-check-again

Id Subject Object Predicate Lexical cue
T5210 733-740 Protein denotes β-actin
T5211 868-873 Protein denotes TNF-α
T5212 1004-1010 Protein denotes ICAM-1

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T5213 167-194 Protein denotes M-MLV reverse transcriptase
T5214 449-472 Protein denotes Goldstar DNA polymerase
T5215 507-530 Protein denotes Goldstar DNA polymerase
T5216 733-740 Protein denotes β-actin
T5217 868-873 Protein denotes TNF-α
T5218 1004-1010 Protein denotes ICAM-1

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T4742 733-740 Protein denotes β-actin
T4743 868-873 Protein denotes TNF-α
T4744 1004-1010 Protein denotes ICAM-1

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T4947 733-740 P60709 denotes β-actin
T4948 868-871 P01375 denotes TNF
T4949 868-873 P01375 denotes TNF-α
T4950 1004-1010 P05362 denotes ICAM-1

test2

Id Subject Object Predicate Lexical cue
T4739 733-740 Protein denotes β-actin
T4740 868-873 Protein denotes TNF-α
T4741 1004-1010 Protein denotes ICAM-1