PMC:2800179 / 10154-10961 JSON TXT

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GO-CC

{"project":"GO-CC","denotations":[{"id":"T4547","span":{"begin":0,"end":4},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T4548","span":{"begin":77,"end":82},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T4549","span":{"begin":214,"end":219},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T4550","span":{"begin":336,"end":341},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T4551","span":{"begin":584,"end":589},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T4552","span":{"begin":801,"end":806},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T4553","span":{"begin":0,"end":12},"obj":"http://purl.obolibrary.org/obo/GO_0009986"},{"id":"T4554","span":{"begin":370,"end":380},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T4555","span":{"begin":470,"end":478},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T4556","span":{"begin":370,"end":380},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T4557","span":{"begin":470,"end":478},"obj":"http://purl.obolibrary.org/obo/GO_0042571"}],"text":"Cell Surface Antigen Expression Analysis\nPeripheral Blood Monocytes or THP-1 cells were exposed to complete medium in the presence or absence of carrageenan for 36 h. After two washes in PBS without Ca2+ and Mg2+, cells were incubated in PBS containing 0.1% gelatin and 8% AB human serum to prevent binding to Fc receptors. Then, 5×105 cells were incubated with primary antibodies at 4°C for 30 min. Two other washes in PBS preceded incubation with FITC-conjugated goat antibody anti-mouse IgG diluted 1/1000 at 4°C for 30 min (Tebu). After two additional washes, analysis of stained cells was performed on an EPICS XL2 (Beckman-Coulter). The cell population was gated according to its forward and wide-angle light scattering. Data were expressed as mean relative fluorescence intensity (MFI) of 3000 cells."}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T4544","span":{"begin":299,"end":306},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T4545","span":{"begin":370,"end":380},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T4546","span":{"begin":470,"end":478},"obj":"http://purl.obolibrary.org/obo/GO_0003823"}],"text":"Cell Surface Antigen Expression Analysis\nPeripheral Blood Monocytes or THP-1 cells were exposed to complete medium in the presence or absence of carrageenan for 36 h. After two washes in PBS without Ca2+ and Mg2+, cells were incubated in PBS containing 0.1% gelatin and 8% AB human serum to prevent binding to Fc receptors. Then, 5×105 cells were incubated with primary antibodies at 4°C for 30 min. Two other washes in PBS preceded incubation with FITC-conjugated goat antibody anti-mouse IgG diluted 1/1000 at 4°C for 30 min (Tebu). After two additional washes, analysis of stained cells was performed on an EPICS XL2 (Beckman-Coulter). The cell population was gated according to its forward and wide-angle light scattering. Data were expressed as mean relative fluorescence intensity (MFI) of 3000 cells."}

pmc-enju-pas

bionlp-st-ge-2016-spacy-parsed

UBERON-AE

{"project":"UBERON-AE","denotations":[{"id":"T4232","span":{"begin":52,"end":57},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"T4233","span":{"begin":282,"end":287},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"Cell Surface Antigen Expression Analysis\nPeripheral Blood Monocytes or THP-1 cells were exposed to complete medium in the presence or absence of carrageenan for 36 h. After two washes in PBS without Ca2+ and Mg2+, cells were incubated in PBS containing 0.1% gelatin and 8% AB human serum to prevent binding to Fc receptors. Then, 5×105 cells were incubated with primary antibodies at 4°C for 30 min. Two other washes in PBS preceded incubation with FITC-conjugated goat antibody anti-mouse IgG diluted 1/1000 at 4°C for 30 min (Tebu). After two additional washes, analysis of stained cells was performed on an EPICS XL2 (Beckman-Coulter). The cell population was gated according to its forward and wide-angle light scattering. Data were expressed as mean relative fluorescence intensity (MFI) of 3000 cells."}

sentences

{"project":"sentences","denotations":[{"id":"T4234","span":{"begin":0,"end":40},"obj":"Sentence"},{"id":"T4235","span":{"begin":41,"end":166},"obj":"Sentence"},{"id":"T4236","span":{"begin":167,"end":323},"obj":"Sentence"},{"id":"T4237","span":{"begin":324,"end":399},"obj":"Sentence"},{"id":"T4238","span":{"begin":400,"end":534},"obj":"Sentence"},{"id":"T4239","span":{"begin":535,"end":638},"obj":"Sentence"},{"id":"T4240","span":{"begin":639,"end":726},"obj":"Sentence"},{"id":"T4241","span":{"begin":727,"end":807},"obj":"Sentence"},{"id":"T88","span":{"begin":0,"end":40},"obj":"Sentence"},{"id":"T89","span":{"begin":41,"end":166},"obj":"Sentence"},{"id":"T90","span":{"begin":167,"end":323},"obj":"Sentence"},{"id":"T91","span":{"begin":324,"end":399},"obj":"Sentence"},{"id":"T92","span":{"begin":400,"end":534},"obj":"Sentence"},{"id":"T93","span":{"begin":535,"end":638},"obj":"Sentence"},{"id":"T94","span":{"begin":639,"end":726},"obj":"Sentence"},{"id":"T95","span":{"begin":727,"end":807},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Cell Surface Antigen Expression Analysis\nPeripheral Blood Monocytes or THP-1 cells were exposed to complete medium in the presence or absence of carrageenan for 36 h. After two washes in PBS without Ca2+ and Mg2+, cells were incubated in PBS containing 0.1% gelatin and 8% AB human serum to prevent binding to Fc receptors. Then, 5×105 cells were incubated with primary antibodies at 4°C for 30 min. Two other washes in PBS preceded incubation with FITC-conjugated goat antibody anti-mouse IgG diluted 1/1000 at 4°C for 30 min (Tebu). After two additional washes, analysis of stained cells was performed on an EPICS XL2 (Beckman-Coulter). The cell population was gated according to its forward and wide-angle light scattering. Data were expressed as mean relative fluorescence intensity (MFI) of 3000 cells."}

bionlp-st-ge-2016-reference-tees

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T4558","span":{"begin":5,"end":20},"obj":"Protein"},{"id":"T4559","span":{"begin":21,"end":31},"obj":"Gene_expression"},{"id":"T4560","span":{"begin":310,"end":322},"obj":"Protein"},{"id":"T4561","span":{"begin":299,"end":306},"obj":"Binding"},{"id":"T4562","span":{"begin":490,"end":493},"obj":"Protein"},{"id":"T4563","span":{"begin":610,"end":619},"obj":"Protein"}],"relations":[{"id":"R4090","pred":"themeOf","subj":"T4558","obj":"T4559"},{"id":"R4091","pred":"themeOf","subj":"T4560","obj":"T4561"}],"text":"Cell Surface Antigen Expression Analysis\nPeripheral Blood Monocytes or THP-1 cells were exposed to complete medium in the presence or absence of carrageenan for 36 h. After two washes in PBS without Ca2+ and Mg2+, cells were incubated in PBS containing 0.1% gelatin and 8% AB human serum to prevent binding to Fc receptors. Then, 5×105 cells were incubated with primary antibodies at 4°C for 30 min. Two other washes in PBS preceded incubation with FITC-conjugated goat antibody anti-mouse IgG diluted 1/1000 at 4°C for 30 min (Tebu). After two additional washes, analysis of stained cells was performed on an EPICS XL2 (Beckman-Coulter). The cell population was gated according to its forward and wide-angle light scattering. Data were expressed as mean relative fluorescence intensity (MFI) of 3000 cells."}

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T4381","span":{"begin":755,"end":763},"obj":"Q04864"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Cell Surface Antigen Expression Analysis\nPeripheral Blood Monocytes or THP-1 cells were exposed to complete medium in the presence or absence of carrageenan for 36 h. After two washes in PBS without Ca2+ and Mg2+, cells were incubated in PBS containing 0.1% gelatin and 8% AB human serum to prevent binding to Fc receptors. Then, 5×105 cells were incubated with primary antibodies at 4°C for 30 min. Two other washes in PBS preceded incubation with FITC-conjugated goat antibody anti-mouse IgG diluted 1/1000 at 4°C for 30 min (Tebu). After two additional washes, analysis of stained cells was performed on an EPICS XL2 (Beckman-Coulter). The cell population was gated according to its forward and wide-angle light scattering. Data were expressed as mean relative fluorescence intensity (MFI) of 3000 cells."}

test2

{"project":"test2","denotations":[{"id":"T4231","span":{"begin":299,"end":306},"obj":"Binding"}],"text":"Cell Surface Antigen Expression Analysis\nPeripheral Blood Monocytes or THP-1 cells were exposed to complete medium in the presence or absence of carrageenan for 36 h. After two washes in PBS without Ca2+ and Mg2+, cells were incubated in PBS containing 0.1% gelatin and 8% AB human serum to prevent binding to Fc receptors. Then, 5×105 cells were incubated with primary antibodies at 4°C for 30 min. Two other washes in PBS preceded incubation with FITC-conjugated goat antibody anti-mouse IgG diluted 1/1000 at 4°C for 30 min (Tebu). After two additional washes, analysis of stained cells was performed on an EPICS XL2 (Beckman-Coulter). The cell population was gated according to its forward and wide-angle light scattering. Data were expressed as mean relative fluorescence intensity (MFI) of 3000 cells."}

PMC:2800179 / 10154-10961 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:2800179 / 10154-10961 JSON TXT