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Vivo Studies\nBALB/c mice were injected intravenously (i.v.) with a red blood cell-depleted single-cell spleen suspension (2.5 × 107 cells) (Castro et al., 2000) from DO11.10 WT or STAT-deficient mice. After 48 hr, they were injected subcutaneously with PBS or with OVA protein (5 mg) plus LPS (5 μg). The inguinal lymph nodes were removed 48 hr later. A single-cell suspension (1 × 106 cells) was restimulated for 24 or 48 hr with 1 μM or 3 μM of OVA and with BrefeldinA for the last 6 hr. Half of the supernatant was removed before the addition of BrefeldinA for use in an ELISA assay. The cells were fixed and stained as before. Those positive for KJ1-26-Bio and for CD4-PerCP were gated, and IFN-γ and IL-10 staining was examined for this population and analyzed as before."}

{"project":"UBERON-AE","denotations":[{"id":"T20230","span":{"begin":74,"end":79},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"T20231","span":{"begin":106,"end":112},"obj":"http://purl.obolibrary.org/obo/UBERON_0002106"},{"id":"T20232","span":{"begin":308,"end":328},"obj":"http://purl.obolibrary.org/obo/UBERON_0001542"},{"id":"T20233","span":{"begin":317,"end":322},"obj":"http://purl.obolibrary.org/obo/UBERON_0002391"},{"id":"T20234","span":{"begin":317,"end":328},"obj":"http://purl.obolibrary.org/obo/UBERON_0000029"}],"text":"In Vivo Studies\nBALB/c mice were injected intravenously (i.v.) with a red blood cell-depleted single-cell spleen suspension (2.5 × 107 cells) (Castro et al., 2000) from DO11.10 WT or STAT-deficient mice. After 48 hr, they were injected subcutaneously with PBS or with OVA protein (5 mg) plus LPS (5 μg). The inguinal lymph nodes were removed 48 hr later. A single-cell suspension (1 × 106 cells) was restimulated for 24 or 48 hr with 1 μM or 3 μM of OVA and with BrefeldinA for the last 6 hr. Half of the supernatant was removed before the addition of BrefeldinA for use in an ELISA assay. The cells were fixed and stained as before. Those positive for KJ1-26-Bio and for CD4-PerCP were gated, and IFN-γ and IL-10 staining was examined for this population and analyzed as before."}

{"project":"GO-MF","denotations":[{"id":"T20575","span":{"begin":708,"end":713},"obj":"http://purl.obolibrary.org/obo/GO_0005141"}],"text":"In Vivo Studies\nBALB/c mice were injected intravenously (i.v.) with a red blood cell-depleted single-cell spleen suspension (2.5 × 107 cells) (Castro et al., 2000) from DO11.10 WT or STAT-deficient mice. After 48 hr, they were injected subcutaneously with PBS or with OVA protein (5 mg) plus LPS (5 μg). The inguinal lymph nodes were removed 48 hr later. A single-cell suspension (1 × 106 cells) was restimulated for 24 or 48 hr with 1 μM or 3 μM of OVA and with BrefeldinA for the last 6 hr. Half of the supernatant was removed before the addition of BrefeldinA for use in an ELISA assay. The cells were fixed and stained as before. Those positive for KJ1-26-Bio and for CD4-PerCP were gated, and IFN-γ and IL-10 staining was examined for this population and analyzed as before."}

{"project":"GO-CC","denotations":[{"id":"T20576","span":{"begin":80,"end":84},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T20577","span":{"begin":101,"end":105},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T20578","span":{"begin":364,"end":368},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T20579","span":{"begin":389,"end":394},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T20580","span":{"begin":594,"end":599},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"In Vivo Studies\nBALB/c mice were injected intravenously (i.v.) with a red blood cell-depleted single-cell spleen suspension (2.5 × 107 cells) (Castro et al., 2000) from DO11.10 WT or STAT-deficient mice. After 48 hr, they were injected subcutaneously with PBS or with OVA protein (5 mg) plus LPS (5 μg). The inguinal lymph nodes were removed 48 hr later. A single-cell suspension (1 × 106 cells) was restimulated for 24 or 48 hr with 1 μM or 3 μM of OVA and with BrefeldinA for the last 6 hr. Half of the supernatant was removed before the addition of BrefeldinA for use in an ELISA assay. The cells were fixed and stained as before. Those positive for KJ1-26-Bio and for CD4-PerCP were gated, and IFN-γ and IL-10 staining was examined for this population and analyzed as before."}

{"project":"sentences","denotations":[{"id":"T20243","span":{"begin":0,"end":15},"obj":"Sentence"},{"id":"T20244","span":{"begin":16,"end":203},"obj":"Sentence"},{"id":"T20245","span":{"begin":204,"end":303},"obj":"Sentence"},{"id":"T20246","span":{"begin":304,"end":354},"obj":"Sentence"},{"id":"T20247","span":{"begin":355,"end":492},"obj":"Sentence"},{"id":"T20248","span":{"begin":493,"end":589},"obj":"Sentence"},{"id":"T20249","span":{"begin":590,"end":633},"obj":"Sentence"},{"id":"T20250","span":{"begin":634,"end":779},"obj":"Sentence"},{"id":"T178","span":{"begin":0,"end":15},"obj":"Sentence"},{"id":"T179","span":{"begin":16,"end":203},"obj":"Sentence"},{"id":"T180","span":{"begin":204,"end":303},"obj":"Sentence"},{"id":"T181","span":{"begin":304,"end":354},"obj":"Sentence"},{"id":"T182","span":{"begin":355,"end":492},"obj":"Sentence"},{"id":"T183","span":{"begin":493,"end":589},"obj":"Sentence"},{"id":"T184","span":{"begin":590,"end":633},"obj":"Sentence"},{"id":"T185","span":{"begin":634,"end":779},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"In Vivo Studies\nBALB/c mice were injected intravenously (i.v.) with a red blood cell-depleted single-cell spleen suspension (2.5 × 107 cells) (Castro et al., 2000) from DO11.10 WT or STAT-deficient mice. After 48 hr, they were injected subcutaneously with PBS or with OVA protein (5 mg) plus LPS (5 μg). The inguinal lymph nodes were removed 48 hr later. A single-cell suspension (1 × 106 cells) was restimulated for 24 or 48 hr with 1 μM or 3 μM of OVA and with BrefeldinA for the last 6 hr. Half of the supernatant was removed before the addition of BrefeldinA for use in an ELISA assay. The cells were fixed and stained as before. Those positive for KJ1-26-Bio and for CD4-PerCP were gated, and IFN-γ and IL-10 staining was examined for this population and analyzed as before."}

{"project":"events-check-again","denotations":[{"id":"T20597","span":{"begin":183,"end":187},"obj":"Protein"},{"id":"T20598","span":{"begin":188,"end":197},"obj":"Negative_regulation"},{"id":"T20599","span":{"begin":268,"end":271},"obj":"Protein"},{"id":"T20600","span":{"begin":450,"end":453},"obj":"Protein"},{"id":"T20601","span":{"begin":653,"end":659},"obj":"Protein"},{"id":"T20602","span":{"begin":672,"end":675},"obj":"Protein"},{"id":"T20603","span":{"begin":698,"end":703},"obj":"Protein"},{"id":"T20604","span":{"begin":708,"end":713},"obj":"Protein"}],"relations":[{"id":"R16334","pred":"themeOf","subj":"T20597","obj":"T20598"}],"text":"In Vivo Studies\nBALB/c mice were injected intravenously (i.v.) with a red blood cell-depleted single-cell spleen suspension (2.5 × 107 cells) (Castro et al., 2000) from DO11.10 WT or STAT-deficient mice. After 48 hr, they were injected subcutaneously with PBS or with OVA protein (5 mg) plus LPS (5 μg). The inguinal lymph nodes were removed 48 hr later. A single-cell suspension (1 × 106 cells) was restimulated for 24 or 48 hr with 1 μM or 3 μM of OVA and with BrefeldinA for the last 6 hr. Half of the supernatant was removed before the addition of BrefeldinA for use in an ELISA assay. The cells were fixed and stained as before. Those positive for KJ1-26-Bio and for CD4-PerCP were gated, and IFN-γ and IL-10 staining was examined for this population and analyzed as before."}

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T20605","span":{"begin":169,"end":179},"obj":"Protein"},{"id":"T20606","span":{"begin":183,"end":187},"obj":"Protein"},{"id":"T20607","span":{"begin":188,"end":197},"obj":"Negative_regulation"},{"id":"T20608","span":{"begin":188,"end":197},"obj":"Negative_regulation"},{"id":"T20609","span":{"begin":552,"end":562},"obj":"Protein"},{"id":"T20610","span":{"begin":672,"end":675},"obj":"Protein"},{"id":"T20611","span":{"begin":676,"end":681},"obj":"Protein"},{"id":"T20612","span":{"begin":698,"end":703},"obj":"Protein"},{"id":"T20613","span":{"begin":708,"end":713},"obj":"Protein"}],"relations":[{"id":"R16335","pred":"themeOf","subj":"T20605","obj":"T20607"},{"id":"R16336","pred":"themeOf","subj":"T20606","obj":"T20608"}],"text":"In Vivo Studies\nBALB/c mice were injected intravenously (i.v.) with a red blood cell-depleted single-cell spleen suspension (2.5 × 107 cells) (Castro et al., 2000) from DO11.10 WT or STAT-deficient mice. After 48 hr, they were injected subcutaneously with PBS or with OVA protein (5 mg) plus LPS (5 μg). The inguinal lymph nodes were removed 48 hr later. A single-cell suspension (1 × 106 cells) was restimulated for 24 or 48 hr with 1 μM or 3 μM of OVA and with BrefeldinA for the last 6 hr. Half of the supernatant was removed before the addition of BrefeldinA for use in an ELISA assay. The cells were fixed and stained as before. Those positive for KJ1-26-Bio and for CD4-PerCP were gated, and IFN-γ and IL-10 staining was examined for this population and analyzed as before."}

{"project":"test2","denotations":[{"id":"T20221","span":{"begin":183,"end":187},"obj":"Protein"},{"id":"T20222","span":{"begin":188,"end":197},"obj":"Negative_regulation"},{"id":"T20223","span":{"begin":268,"end":271},"obj":"Protein"},{"id":"T20224","span":{"begin":450,"end":453},"obj":"Protein"},{"id":"T20225","span":{"begin":653,"end":659},"obj":"Protein"},{"id":"T20226","span":{"begin":672,"end":675},"obj":"Protein"},{"id":"T20227","span":{"begin":698,"end":703},"obj":"Protein"},{"id":"T20228","span":{"begin":708,"end":713},"obj":"Protein"},{"id":"T20229","span":{"begin":714,"end":722},"obj":"Gene_expression"}],"relations":[{"id":"R16010","pred":"themeOf","subj":"T20221","obj":"T20222"},{"id":"R16011","pred":"themeOf","subj":"T20228","obj":"T20229"}],"text":"In Vivo Studies\nBALB/c mice were injected intravenously (i.v.) with a red blood cell-depleted single-cell spleen suspension (2.5 × 107 cells) (Castro et al., 2000) from DO11.10 WT or STAT-deficient mice. After 48 hr, they were injected subcutaneously with PBS or with OVA protein (5 mg) plus LPS (5 μg). The inguinal lymph nodes were removed 48 hr later. A single-cell suspension (1 × 106 cells) was restimulated for 24 or 48 hr with 1 μM or 3 μM of OVA and with BrefeldinA for the last 6 hr. Half of the supernatant was removed before the addition of BrefeldinA for use in an ELISA assay. The cells were fixed and stained as before. Those positive for KJ1-26-Bio and for CD4-PerCP were gated, and IFN-γ and IL-10 staining was examined for this population and analyzed as before."}