PMC:2783367 / 9693-11203
Annnotations
{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/2783367","sourcedb":"PMC","sourceid":"2783367","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2783367","text":"The lox-1 promoter luciferase reporter plasmid (p-2336/+36-Luc), containing a 2336 bp fragment of the 5′-flanking promoter region of the LOX-1 gene, and some of the reporter plasmids with various lengths of the lox-1 promoter were kindly provided by Dr. Jawahar L. Mehta (Department of Internal Medicine, University of Arkansas for Medical Sciences) 29. Additional lox-1 promoter luciferase reporter constructs with shorter promoter regions were created by PCR and enzyme ligation using p-2336/+36-Luc as a template (see the following for details). The PDGF-βR promoter luciferase reporter plasmid pPDGF-βR-Luc (pβ12) was a gift from Dr. Keiko Funa (Ludwig Institute for Cancer Research, Uppsala, Sweden) 30. The type I TGF-β receptor promoter luciferase reporter plasmid pTβ-RI-Luc (pES1.0) was kindly provided by Dr Michael Centrella (Yale University, New Haven, CT, USA) 31. The PPARγ cDNA expression plasmid pPPARγ, containing a full size of PPARγ cDNA, was a gift from Dr. Reed Graves (Department of Medicine, University of Chicago). The dominant negative PPARγ expression construct pdn-PPARγ was a gift from Dr. Krishna V. Chatterjee 32. The Wnt signaling luciferase reporter plasmids TOPflash and its mutant FOPflash were kindly provided by Dr. Randall T. Moon (Department of Pharmacology, School of Medicine, University of Washington) 33. TOPflash contained 8 copies of TCF/LEF binding sites. 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