PMC:2783367 / 63189-64656 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/2783367","sourcedb":"PMC","sourceid":"2783367","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2783367","text":"The activation of PPARγ by curcumin interrupts canonical Wnt signaling in activated HSCs in vitro\n(A) and (B): HSCs were transiently transfected with the plasmid TOPflash or FOPflash. After recovery, cells were incubated in DMEM with FBS (10%) with treatment for 24 hr. *P\u003c 0.05 vs. cells with no treatment (the first column). Luciferase activities were expressed as relative units after β-galactosidase normalization (means ± s.d.) (n=6). The floating schemas denote the plasmid TOPflash or its mutant FOPflash in use and the application of treatments to the system. (A). Luciferase activity assays of cells pretreated with PD68235 (20μM) for 30min prior to the addition of curcumin (20μM) for additional 24 hr. (B). Luciferase activity assays of cells co-transfected with pPPARγ at indicated doses. A total of 4.5μg of plasmid DNA per well was used for co-transfection of HSCs in 6-well culture plates. It included 2μg of TOPflash, or FOPflash, 0.5μg of pSV-β-gal and 2.0μg of pPPARγ plus pcDNA. The latter was used to ensure an equal amount of total DNA in transfection assays. (C). HSCs were transfected with TOPflash and treated with PGJ2 at indicated doses in serum-depleted medium for 24 hr. Luciferase activities were expressed as relative units after β-galactosidase normalization (means ± s.d.) (n=6). *P\u003c 0.05 vs. cells with no treatment (the first column). The floating schema denoted the plasmid TOPflash in use and the application of PGJ2 to the system.","divisions":[{"label":"title","span":{"begin":0,"end":97}}],"tracks":[]}