PMC:2783367 / 61516-63177 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/2783367","sourcedb":"PMC","sourceid":"2783367","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2783367","text":"(A). Luciferase activity assays of HSCs transiently transfected with the plasmid TOPflash or FOPflash, followed by the treatment with curcumin at indicated concentrations for 24 hr. (n=6). *P\u003c 0.05 vs. cells with no treatment (the first column). The floating schema denoted the canonical Wnt signaling luciferase reporter construct TOPflash or its mutant counterpart FOPflash in use and the application of curcumin to the system. (B). Semi-confluent HSCs were treated with curcumin at indicated concentrations for 24 hr. Total nuclear extracts were prepared for Western blotting analyses of β-catenin. Histone H1 was used as an invariant control for equal nuclear protein loading. Representative was from three independent experiments. Italic numbers beneath the blot were fold changes in the densities of the bands compared to the control without treatment in the blot (n=3), after normalization with Histone H1. Because of the limited space, standard deviations were not presented. (C). EMSA of nuclear protein extracts from HSCs treated with various concentrations of curcumin using the biotin-labeled probe P(TCFwt), which contained the consensus TCF/LEF binding site found in the lox-1 promoter. (D). EMSA competition assays of nuclear protein extracts from HSCs treated with or without curcumin (Cur) at 20 μM using the biotin-labeled probe P(TCFwt) and a 10-, 50-, or 100-fold excess of the unlabeled P(TCFwt) (lanes 3-5), or an unlabeled probe P(TCFmut) (lanes 6-8). The latter probe contained the consensus TCF/LEF binding site found in the lox-1 promoter with site-directed mutations. Representatives of EMSA were shown from 3 independent experiments.","tracks":[]}