PMC:2783367 / 35507-36601 JSONTXT

{"project":"2_test","denotations":[{"id":"19736547-15817814-66178371","span":{"begin":523,"end":525},"obj":"15817814"},{"id":"19736547-15817814-66178371","span":{"begin":523,"end":525},"obj":"15817814"},{"id":"19736547-15817814-66178372","span":{"begin":636,"end":638},"obj":"15817814"},{"id":"19736547-15817814-66178372","span":{"begin":636,"end":638},"obj":"15817814"},{"id":"T22352","span":{"begin":523,"end":525},"obj":"15817814"},{"id":"T21050","span":{"begin":523,"end":525},"obj":"15817814"},{"id":"T65912","span":{"begin":636,"end":638},"obj":"15817814"},{"id":"T33215","span":{"begin":636,"end":638},"obj":"15817814"}],"text":"To explore the mechanisms by which curcumin eliminated the stimulatory effect of Wnt signaling on the induction of lox-1 expression, we assumed that the activation of PPARγ by curcumin antagonistically interacted with the Wnt signaling pathway and led to the interruption of canonical Wnt signaling in activated HSCs. To test the assumption, passaged HSCs were transfected with the plasmid TOPflash, or FOPflash. TOPflash was a canonical Wnt signaling luciferase reporter, which contained 8 copies of TCF/LEF binding sites 33. FOPflash was used as a control luciferase reporter, which contained 8 copies of mutant TCF/LEF binding sites 33. After recovery, cells were treated with curcumin at various concentrations (0-30 μM) in DMEM with FBS (10%) for 24 hr. Luciferase activity assays in Fig. 9A demonstrated that curcumin caused a dose-dependent reduction in luciferase activities in cells transfected with TOPflash. However, curcumin had no impact on luciferase activities in cells transfected with FOPflash. These results suggested that curcumin interrupted canonical Wnt signaling in HSCs."}