PMC:2783367 / 33909-34768 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/2783367","sourcedb":"PMC","sourceid":"2783367","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2783367","text":"Additional experiments were conducted to clarify the impact of canonical Wnt signaling on regulating lox-1 expression in activated HSCs. HSCs were transfected with the lox-1 promoter luciferase reporter plasmid pLOX-1-Luc. After recovery, cells were serum-starved for 3 hr prior to the stimulation with Wnt3a at 0-100 ng/ml in serum-free medium for additional 24 hr. Luciferase activity assays demonstrated that the activation of canonical Wnt signaling by exogenous Wnt3a dose-dependently increased luciferase activities in the cells (Fig. 8A), suggesting the stimulatory effect of Wnt signaling on the lox-1 promoter activity. This observation was confirmed by Western blotting analyses in serum-starved HSCs treated with Wnt3a at 0-100 ng/ml in serum-depleted media for 24 hr (Fig. 8B). Wnt3a dose-dependently increased the abundance of LOX-1 in the cells.","tracks":[]}