PMC:2783367 / 18262-19687 JSONTXT

{"project":"2_test","denotations":[{"id":"19736547-16324688-66178352","span":{"begin":122,"end":123},"obj":"16324688"},{"id":"19736547-16324688-66178352","span":{"begin":122,"end":123},"obj":"16324688"},{"id":"19736547-9052782-66178353","span":{"begin":125,"end":126},"obj":"9052782"},{"id":"19736547-9052782-66178353","span":{"begin":125,"end":126},"obj":"9052782"},{"id":"19736547-9933026-66178354","span":{"begin":297,"end":299},"obj":"9933026"},{"id":"19736547-9933026-66178354","span":{"begin":297,"end":299},"obj":"9933026"},{"id":"19736547-10764682-66178355","span":{"begin":344,"end":345},"obj":"10764682"},{"id":"19736547-10764682-66178355","span":{"begin":344,"end":345},"obj":"10764682"},{"id":"19736547-16690797-66178356","span":{"begin":616,"end":618},"obj":"16690797"},{"id":"19736547-16690797-66178356","span":{"begin":616,"end":618},"obj":"16690797"},{"id":"19736547-14656932-66178357","span":{"begin":620,"end":622},"obj":"14656932"},{"id":"19736547-14656932-66178357","span":{"begin":620,"end":622},"obj":"14656932"},{"id":"T38286","span":{"begin":122,"end":123},"obj":"16324688"},{"id":"T84215","span":{"begin":122,"end":123},"obj":"16324688"},{"id":"T71924","span":{"begin":125,"end":126},"obj":"9052782"},{"id":"T73957","span":{"begin":125,"end":126},"obj":"9052782"},{"id":"T45969","span":{"begin":297,"end":299},"obj":"9933026"},{"id":"T53662","span":{"begin":297,"end":299},"obj":"9933026"},{"id":"T12503","span":{"begin":344,"end":345},"obj":"10764682"},{"id":"T38917","span":{"begin":344,"end":345},"obj":"10764682"},{"id":"T22777","span":{"begin":616,"end":618},"obj":"16690797"},{"id":"T48696","span":{"begin":616,"end":618},"obj":"16690797"},{"id":"T52436","span":{"begin":620,"end":622},"obj":"14656932"},{"id":"T39936","span":{"begin":620,"end":622},"obj":"14656932"}],"text":"LOX-1 has been identified as a specific receptor for ox-LDL, responsible for transporting extracellular ox-LDL into cells 6, 7. LOX-1, as a scavenger receptor, facilitates the accumulation of ox-LDL and stimulates the transformation of smooth muscle cells and monocyte/macrophages into foam cells 39. LOX-1 gene expression is induced by ox-LDL 9. We assumed that LOX-1 mediated the stimulatory effects of ox-LDL on HSC activation and fibrogenesis. To elucidate the link role of LOX-1 in ox-LDL-induced HSC activation, serum-starved HSCs were pre-treated with or without κ-carrageenan (250 μg/ml), a LOX-1 antagonist 40, 41, for 1 hr prior to the stimulation with ox-LDL (10 μg/ml) for additional 24 hr. Whole cell extracts were prepared for Western blotting analyses of genes relevant to HSC activation. As shown in Fig. 2A, compared with the untreated control (the corresponding 1st wells), ox-LDL, as expected, significantly increased the abundance of proteins closely relevant to HSC activation, including pro-mitogenic PDGF-βR and EGFR and pro-fibrogenic CTGF, Tβ-RI and Tβ-RII (the corresponding 2nd wells). It was of interest to observe that the pretreatment with the LOX-1 antagonist κ-carrageenan dramatically eliminated the stimulatory effects of ox-LDL on the abundance of the proteins (the corresponding 4th wells). These results suggested a direct link of LOX-1 between ox-LDL and HSC activation and fibrogenesis."}