PMC:2783367 / 13733-14377 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/2783367","sourcedb":"PMC","sourceid":"2783367","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2783367","text":"Total RNA was extracted from cells using Trizol reagent (Invitrogen) following the manufacturer's instruction. Real-time PCR was carried out using SYBR green, as previously described 34. Total RNA was treated with DNase I prior to the synthesis of the first strand of cDNA. First-strand cDNA was synthesized using total RNA as templates and oligo-dT as primers. Samples were run on a Bio-Rad MyiQ™ real-time PCR machine (Bio-Rad). mRNA levels were expressed as fold changes after normalization with endogenous glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as suggested by Schmittgen et. al. 35. The primers for PCR were presented in Table 3.","tracks":[{"project":"2_test","denotations":[{"id":"19736547-12223099-66178347","span":{"begin":183,"end":185},"obj":"12223099"},{"id":"19736547-12223099-66178347","span":{"begin":183,"end":185},"obj":"12223099"},{"id":"19736547-11017702-66178348","span":{"begin":594,"end":596},"obj":"11017702"},{"id":"19736547-11017702-66178348","span":{"begin":594,"end":596},"obj":"11017702"},{"id":"T43408","span":{"begin":183,"end":185},"obj":"12223099"},{"id":"T98568","span":{"begin":183,"end":185},"obj":"12223099"},{"id":"T22501","span":{"begin":594,"end":596},"obj":"11017702"},{"id":"T7271","span":{"begin":594,"end":596},"obj":"11017702"}],"attributes":[{"subj":"19736547-12223099-66178347","pred":"source","obj":"2_test"},{"subj":"19736547-12223099-66178347","pred":"source","obj":"2_test"},{"subj":"19736547-11017702-66178348","pred":"source","obj":"2_test"},{"subj":"19736547-11017702-66178348","pred":"source","obj":"2_test"},{"subj":"T43408","pred":"source","obj":"2_test"},{"subj":"T98568","pred":"source","obj":"2_test"},{"subj":"T22501","pred":"source","obj":"2_test"},{"subj":"T7271","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#d9ec93","default":true}]}]}}