PMC:2779105 / 117587-118908 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2779105","sourcedb":"PMC","sourceid":"2779105","source_url":"http://www.ncbi.nlm.nih.gov/pmc/2779105","text":"10.1371/journal.pone.0008058.g012 Figure 12 Energy-dispersive X-ray spectroscopy of protein-mineral nanoparticles shows elemental compositions indistinguishable from those of calcium granules and NB.\nProtein-mineral nanoparticles were obtained as in Fig. 9, from solutions containing BSF (A), HSA (B), or both (C), to which 0.3 mM each of CaCl2 and NaH2PO4 was added, followed by incubation in cell culture conditions for 1 month. EDX spectra were also obtained for calcium granules prepared by adding either CaCl2 (D), NaH2PO4 (E), or a combination of both (F) to FBS, followed by overnight incubation, centrifugation, and washing, as described in the Materials and Methods . NB were cultured from 10% HS (G, “HS-NB”) or from 10% FBS (H and I, corresponding to “Nanons” and “DSM 5820”, respectively). In these specimens, major peaks of carbon, oxygen, calcium, and phosphorus were noted, concordant with the presence of a calcium phosphate mineral containing carbonate. The three controls CaCO3 (J), Ca3(PO4)2 (K), and HAP (L), diluted and washed in double-distilled water, were shown for comparison. The following Ca/P ratios were obtained: (A) 1.37; (B) 1.53; (C) 1.6; (D) 1.32; (E) 1.48; (F) 1.14; (G) 1.48; (H) 1.4; (I) 1.27; (K) 2.54; and (L) 1.48. Phosphorus was not detected in the CaCO3 samples shown in (J). ","divisions":[{"label":"Title","span":{"begin":45,"end":200}}],"tracks":[]}