PMC:2728246 / 9372-9808 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2728246","sourcedb":"PMC","sourceid":"2728246","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2728246","text":"Sample for dataset 13: The equivalent of 5 mg Pf1 phage (ASLA Ltd., Riga, Lativa) was suspended in buffer (50 mM NaPO4, 100 mM NaCl, pH = 6.5, 0.05% NaN3) and pelleted three times at 140,000 g for 1 h. The pellet was then carefully suspended in a 15N,13C-labeled human ubiquitin sample (~2 mg) in the same buffer. NaCl salt was later added to a final concentration of about 300 mM. The water deuterium quadrupolar splitting was 15.8 Hz.","tracks":[]}