PMC:2728246 / 23663-24385 JSONTXT

{"project":"2_test","denotations":[{"id":"19652920-11430758-56190973","span":{"begin":250,"end":254},"obj":"11430758"}],"text":"The magnitude of errors is larger for methyl groups than for NH backbone amide groups (errors for DNH were estimated to be 0.3 Hz or less). The first reason is a limited resolution of the RDC measurements using a constant-time HSQC (Kontaxis and Bax 2001) with the limited maximum evolution time corresponding to approximately 15 Hz resolution compared to better than 2 Hz resolution for NH groups measured on a normal HSQC (without zero filling). Secondly, the coverage of the five-dimensional RDC space is less complete than for the NH measurements, as indicated by a lower 5th eigenvalue of the F-matrix. Although the combined effect of both errors propagates, a quantitative analysis of the results was still possible."}