PMC:2728246 / 11659-12461
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2728246","sourcedb":"PMC","sourceid":"2728246","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2728246","text":"Sub-spectra were separated, processed and co-added to yield four spectra with single peak components corresponding to the ααα, ααβ/αβα/βαα, αββ/βαβ/ββα, or βββ coherence of each methyl group. Couplings (1JCH and 1JCH + 1DCH) were evaluated from the separation between the four components; for precise measurement, a dedicated FELIX script was designed to perform the spectral shifts necessary to simultaneously overlay the four components. Uncertainties on the couplings have been estimated as the range of shifts for which 4-peak overlays was acceptable. Assuming fast axial reorientation, each measured methyl CH RDC (1DCH3) was rescaled to the methyl CC RDC (1DCC), corresponding to the CC bond co-linear to the rotation axis, using a universal factor of −0.3155 according to (Ottiger and Bax 1999).","tracks":[]}