PMC:2728203 / 15269-15925 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2728203","sourcedb":"PMC","sourceid":"2728203","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2728203","text":"We also obtained P43212 symmetry crystals of the Nab2 N-terminal domain that diffracted past 1.8 Å resolution in-house (Table 2). We used the solution NMR structure of this domain as a model for molecular replacement and, after trying a number of different models containing different fragments of the structure, were able to obtain a unique solution using residues 7–82 (corresponding to helices H1–H4). After solvent flattening, the electron density enabled residues 6–94 to be built to produce a structural model that had an R-factor of 20.7% (Rfree = 26.4%) and excellent geometry (Table 2) after iterative cycles of refinement and rebuilding (Fig. 3).","tracks":[]}