PMC:2724173 / 9455-10827 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2724173","sourcedb":"PMC","sourceid":"2724173","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2724173","text":"Pallen et al. used multiple sequence alignments to identify members of the MxiC/YopN–TyeA family from divergent Gram-negative species;7 a structure-based sequence alignment of MxiC and YopN–TyeA (Fig. 3a) differs in the N-terminal region from the alignment obtained in that work, and highlights the need to be cautious when interpreting conservation based on sequence alignments alone. The combination of structural and sequence alignment information identifies that most of the highly conserved residues are involved in maintaining the correct fold of these proteins. In support of this, mutations of YopN that constitutively block secretion replace highly conserved residues that are buried in the interfaces between domains of YopN.23 Ferracci et al. suggested that these mutations are likely to cause part of YopN to become unfolded or less well-ordered.23 Interestingly, mapping of sequence conservation onto the structure across the more closely related Salmonella, Burkholderia and Shigella species reveals a hydrophobic patch on the surface of the central domain consisting of residues Leu222, Met226, Gly239, Leu242 and Leu245 (Fig. 3b, red circle). This region is buried by crystal contacts in all seven independent molecules of MxiC in the P212121, P222 and P43212 crystal forms, suggesting that this region may be a “hot spot” for protein–protein interactions.","tracks":[{"project":"2_test","denotations":[{"id":"18304577-15757514-62526549","span":{"begin":134,"end":135},"obj":"15757514"},{"id":"18304577-16091038-62526550","span":{"begin":735,"end":737},"obj":"16091038"},{"id":"18304577-16091038-62526551","span":{"begin":858,"end":860},"obj":"16091038"}],"attributes":[{"subj":"18304577-15757514-62526549","pred":"source","obj":"2_test"},{"subj":"18304577-16091038-62526550","pred":"source","obj":"2_test"},{"subj":"18304577-16091038-62526551","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#93e6ec","default":true}]}]}}