PMC:2724026 / 39364-41119 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2724026","sourcedb":"PMC","sourceid":"2724026","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2724026","text":"Table 2 Folding data for FADD DD core mutants\nFADD DD variant Core probed by mutation ΔGD-N (kcal mol-1) ΔΔGD-N (kcal mol-1) mD-N (kcal mol-1 M-1) kf2M (s-1) Φ2M\nWT 6.7 1.4 40\nHelix1\nPhe101Ala Central 4.2 2.6 1.6 10 0.31\nVal103Ala B1 5.3 1.4 1.2 36 0.04\nIle104Ala Central 4.3 2.4 1.1 12 0.29\nHelix2\nTrp112Ala B2 2.8 3.9 1.4 10 0.18\nLeu115Met Central 5.1 2.4 1.4 22 0.22\nLeu119Met Central 4.3 1.6 1.3 28 0.10\nHelix3\nIle126Ala B2 5.6 1.2 1.2 36 0.05\nIle129Ala B2 4.5 2.2 1.5 43 –0.02\nTyr133Ala B2 4.5 2.2 1.1 39 0.01\nHelix4\nArg140Ala B2 7.3 –0.6 1.1 72 0.60\nVal141Ala Central 5.5 1.3 1.3 14 0.51\nSer144Ala B2 7.4 – 0.7 2.1 114 0.86\nLeu145Met Central 4.5 2.3 1.4 23 0.15\nTrp148Phe Central 3.6 3.1 1.4 55 –0.06\nHelix5\nHis160Ala Central 7.7 –1.0 1.3 80 0.40\nLeu161Ala Central 2.9 3.9 1.6 4 0.37\nVal162Ala B1 4.9 1.9 1.5 16 0.29\nLeu165Ala B1 2.8 3.9 1.6 7 0.26\nCys168Ala Central 6.4 0.4 1.4 42 n.d.\nHelix6\nVal173Ala B1 5.2 1.1 1.4 29 0.17\nLeu176Ala B1 6.0 0.8 1.3 25 0.37\nVal177Ala B1 4.0 2.7 1.4 24 0.11\nVal180Ala B1 6.8 –0.1 1.2 47 n.d.\nFor clarity, errors are not shown in the table. The error in free energy measurements is in the range ± 0.1-0.2 kcal mol- 1, the error in kf2M is ± 10%, the error in Φ2M \u003c 0.1. Note that Φ-values cannot be considered reliable if the ΔΔGD-N is low27, so Φ-values were not determined where ΔΔGD-N \u003c 0.6 kcal mol- 1 (n.d).\nWe note that a condition of Φ-value analysis is that conservative mutations should be made30; where this is not possible, for example, Trp to Phe, these results should be interpreted with caution and in the context of the surrounding Φ-values 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