PMC:2724026 / 34663-35117 JSONTXT

Annnotations TAB JSON ListView MergeView

All Φ-values were calculated at 2 M urea using Eq. (4):(4) Φ=ΔΔGD−‡ΔΔGD−Nwhere ΔΔGD-N is the equilibrium constant for unfolding determined from equilibrium denaturation experiments and ΔΔGD-‡ is the change in the free energy difference between the denatured (D) and transition (‡) states for folding upon mutation, calculated as:(5) ΔΔGD−‡=RTln(kfwt/kfmut)kfwt and kfmut are the folding rate constants at 2 M urea for WT and mutant protein, respectively.