PMC:2724026 / 34663-35117 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2724026","sourcedb":"PMC","sourceid":"2724026","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2724026","text":"All Φ-values were calculated at 2 M urea using Eq. (4):(4) Φ=ΔΔGD−‡ΔΔGD−Nwhere ΔΔGD-N is the equilibrium constant for unfolding determined from equilibrium denaturation experiments and ΔΔGD-‡ is the change in the free energy difference between the denatured (D) and transition (‡) states for folding upon mutation, calculated as:(5) ΔΔGD−‡=RTln(kfwt/kfmut)kfwt and kfmut are the folding rate constants at 2 M urea for WT and mutant protein, respectively.","divisions":[{"label":"label","span":{"begin":55,"end":58}},{"label":"label","span":{"begin":329,"end":332}}],"tracks":[]}