PMC:2724026 / 33879-34375
Annnotations
The variation of the logarithm of the observed rate constant with concentration of urea was analysed using Eq. (2); those unfolding rate constants judged to be non-linear were omitted from the fit (see Supplementary Data):(2) lnkobs=ln(kfH2Oexp(−mkf.[urea])+kuH2Oexp(mku.[urea]))where kobs is the observed rate constant, kfH2Oand kuH2O are the folding and unfolding rate constants in 0 M denaturant, respectively, and mkf and mku are the gradients of the folding and unfolding arms, respectively.