PMC:2724026 / 31459-31865 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2724026","sourcedb":"PMC","sourceid":"2724026","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2724026","text":"A synthetic gene corresponding to residues 93–192 of the DD of human FADD, was obtained from overlapping primers using standard PCR techniques; the synthetic gene was inserted into a modified version of pRSETA (Invitrogen) that encodes an N-terminal histidine tag. Site-directed mutagenesis was performed using a Quik Change kit (Stratagene) and the identity of the mutants was confirmed by DNA sequencing.","tracks":[]}